Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr3

Orthogroup ID

OG0010661

Physical Location & Seq

Genomic Coordinates Forward (+)

4115006 .. 4118861

Gene Structure 3856 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom03g05280.11

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1728 bp

ATGTGTGGAATTCTTGCTGTTCTTGGTTGCTCTGATGACTCTCAGGCCAAGAGGGTCCGAGTTCTTGAGCTTTCGCGCAGGTTGAAGCATCGTGGACCTGACTGGAGTGGGCTGTACCAGCATGGAGATTGTTTCTTGGCTCATCAGAGGCTGGCCATCATTGACCCTGCTTCTGGTGATCAACCTCTCTACAACGAGGACAAGTCAATTGTTGTCACGGTGAATGGAGAGATTTACAACCATGAAGAGCTGAGGAGCCGTCTGCCGAATCACAAGTTCCGAACCGGCAGCGATTGTGATGTCATTGCCCATCTGTATGAAGAGTATGGGGAGGATTTCGTTGATATGCTGGACGGCATGTTTTCGTTTGTGCTGCTGGATACCCGCGACAACAGCTTCATCGTTGCTCGCGATGCTATCGGAATCACTTCGCTCTATATCGGCTGGAGTCTTGATGGCTCGGTTTGGATATCATCGGAACTGAAAGGGTTGAATGATGATTGCGAACACTTTGAGAGCTTTCCACCCGGTCATCTGTACTCGAGCAAAGAAGGTGCACTTAAGAGGTGGTACAACCCTACTTGGTTCTCGGAGACCATTCCATCAGCGCCATATGATCCGCTTGTTCTGAGGCGCGCCTTTGAAACTGCTGTGATCAAAAGGCTAATGACTGATGTGCCTTTCGGAGTTCTTCTCTCCGGAGGTCTGGATTCATCTTTGGTCGCCTCTATCACTGCTCGTCACTTGGCAGGCACAAAGGCGGCCAAGCAATGGGGCGCACAGCTCCATTCCTTCTGTGTTGGACTTGAGGGCTCACCTGATCTGAAGGCTGCAAAAGAAGTTGCAGACTATTTGGGAACCGTTCACCACGAGTTTCATTTTACAGTTCAGGATGGAATTGACGCGATCGAGGATGTCATATACCACATAGAAACATACGATGTTACCACCATCCGAGCTAGCACCCCTATGTTTCTCATGTCGCGTAAGATCAAGTCCCTGGGGGTTAAGATGGTGATTTCTGGTGAGGGATCTGATGAAATTTTCGGTGGATACTTGTACTTCCACAAGGCACCCAACAAGGAAGAGTTCCACCGCGAAACCTGCAGAAAGATCAAGGCACTGCACATGTATGATTGCCTCAGGGCAAACAAATCAACATCTGCCTGGGGCTTAGAAGCCCGAGTCCCATTCTTGGACAAGGATTTTATCAAAGCTGCAATGGATATTGATCCCGAGTTTAAAATGATCAAGAAGGACCAAGGAAGAATTGAGAAATGGGTCCTCCGAAAGGCGTTTGATGATGAGGAGCAACCTTATTTGCCGAAGCACATTCTGTACAGGCAGAAGGAGCAGTTCAGCGACGGGGTCGGCTATAGTTGGATTGACGGCCTCAAATCCCATGCTGCTCTCCATGTCACTGATAAGATGATGATGAATGCTGAACGCATCTTCCCCCACAACACTCCATATACAAAGGAGGCCTACTACTACCGAATGATTTTCGAAAGATTCTTCCCACAGAACTCGGCCAGGAACACGGTGCCTGGAGGACCGAGTGTAGCCTGCAGCACAGCCACAGCTGTGGCATGGGATGCCGAATGGTCCAAAAATCTTGACCCATCTGGCAGGGCTATATTTGAAGTGCACCAACAAGCATATGACAAGCAGGGTGCACCGGTGACTAGCGGAATTCCAGATCGATCATTGACAATGTACCTCAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00250 ko01100 ko01110 map00250 map01100 map01110

Pfam Domains

Protein Families

Asn_synthase GATase_7

Protein Analysis

576

Amino Acids

65.1

Weight (kDa)

6.01

Isoelectric Point (pI)

39.92

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010661)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G47340 AT3G47340 AT3G47340 AT3G47340
fragaria_vesca	FvH4_3g38970 FvH4_3g38970
malus_domestica	MD03G1066000.v1.1 MD11G1070800.v1.1
prunus_persica	Prupe.6G054800_v2.0.a1 Prupe.6G054800_v2.0.a1
pyrus_communis	pycom03g05280 pycom11g05920
rosa_chinensis	RchiOBHm_Chr5g0070221
rosa_multiflora	Rmu_sc0002156.1_g000011 Rmu_sc0024476.1_g000001
rosa_roxburghii	Rroxscaffold_1G00010910
rosa_rugosa	Rorug05G0404300
rosa_samantha	Rh5CG502300 Rh5DG490200
rosa_wichuraiana	Rw5G042890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	69, 1112
AccB1I	GGYRCC	2 cut(s)	1072, 1543
AccB7I	CCANNNNNTGG	1 cut(s)	771
AccII	CGCG	7 cut(s)	76, 387, 411, 636, 905, 985, 1098
AccIII	TCCGGA	1 cut(s)	698
AciI	CCGC	5 cut(s)	385, 620, 761, 1096, 1689
AclWI	GGATC	3 cut(s)	611, 1039, 1226
AcoI	YGGCCR	3 cut(s)	153, 762, 1530
AcsI	RAATTY	3 cut(s)	9, 1041, 1692
AcuI	CTGAAG	1 cut(s)	845
AfaI	GTAC	6 cut(s)	116, 539, 572, 1061, 1340, 1718
AfiI	CCNNNNNNNGG	4 cut(s)	173, 771, 1195, 1291
AflII	CTTAAG	1 cut(s)	560
AflIII	ACRYGT	1 cut(s)	1128
AgeI	ACCGGT	1 cut(s)	1678
AgsI	TTSAA	4 cut(s)	85, 493, 644, 1643
AjnI	CCWGG	4 cut(s)	999, 1166, 1532, 1546
AleI	CACNNNNGTG	1 cut(s)	1583
AloI	GAACNNNNNNTCC	2 cut(s)	1437, 1469
AluBI	AGCT	8 cut(s)	70, 250, 396, 519, 784, 959, 1217, 1583
AluI	AGCT	8 cut(s)	70, 250, 396, 519, 784, 959, 1217, 1583
Alw21I	GWGCWC	3 cut(s)	559, 1650, 1678
Alw26I	GTCTC	1 cut(s)	587
Alw44I	GTGCAC	3 cut(s)	555, 1646, 1674
AlwI	GGATC	3 cut(s)	611, 1039, 1226
AlwNI	CAGNNNCTG	1 cut(s)	151
Ama87I	CYCGRG	3 cut(s)	541, 1182, 1235
Aor13HI	TCCGGA	1 cut(s)	698
AoxI	GGCC	6 cut(s)	45, 153, 762, 1390, 1482, 1530
ApaLI	GTGCAC	3 cut(s)	555, 1646, 1674
ApeKI	GCWGC	6 cut(s)	288, 373, 830, 1217, 1406, 1569
ApoI	RAATTY	3 cut(s)	9, 1041, 1692
AscI	GGCGCGCC	1 cut(s)	634
AsiGI	ACCGGT	1 cut(s)	1678
Asp700I	GAANNNNTTC	1 cut(s)	427
AspLEI	GCGC	5 cut(s)	78, 610, 636, 638, 779
AspS9I	GGNCC	6 cut(s)	55, 95, 1258, 1282, 1553, 1605
AsuC2I	CCSGG	1 cut(s)	528
AsuHPI	GGTGA	7 cut(s)	188, 232, 807, 857, 1027, 1037, 1693
AsuII	TTCGAA	1 cut(s)	1506
AsuNHI	GCTAGC	1 cut(s)	959
AvaI	CYCGRG	3 cut(s)	541, 1182, 1235
AvaII	GGWCC	6 cut(s)	55, 95, 1258, 1282, 1553, 1605
AxyI	CCTNAGG	1 cut(s)	1142
BaeGI	GKGCMC	3 cut(s)	559, 1650, 1678
BalI	TGGCCA	1 cut(s)	155
BanI	GGYRCC	2 cut(s)	1072, 1543
BanII	GRGCYC	1 cut(s)	815
BauI	CACGAG	1 cut(s)	869
Bbv12I	GWGCWC	3 cut(s)	559, 1650, 1678
BbvCI	CCTCAGC	1 cut(s)	251
BbvI	GCAGC	6 cut(s)	300, 360, 817, 1204, 1393, 1581
BccI	CCATC	8 cut(s)	164, 318, 449, 610, 887, 959, 1006, 1630
BceAI	ACGGC	3 cut(s)	243, 370, 1405
BciT130I	CCWGG	4 cut(s)	1001, 1168, 1534, 1548
BciVI	GTATCC	2 cut(s)	373, 1046
BclI	TGATCA	3 cut(s)	178, 654, 1248
BcnI	CCSGG	1 cut(s)	528
BcoDI	GTCTC	1 cut(s)	587
BfaI	CTAG	2 cut(s)	960, 1686
BfmI	CTRYAG	3 cut(s)	1105, 1375, 1567
BfoI	RGCGCY	1 cut(s)	611
BfrI	CTTAAG	1 cut(s)	560
BfuAI	ACCTGC	2 cut(s)	69, 1112
BfuI	GTATCC	2 cut(s)	373, 1046
BisI	GCNGC	7 cut(s)	289, 374, 762, 831, 1218, 1407, 1570
BlsI	GCNGC	7 cut(s)	290, 375, 763, 832, 1219, 1408, 1571
Bme1390I	CCNGG	5 cut(s)	528, 1001, 1168, 1534, 1548
Bme18I	GGWCC	6 cut(s)	55, 95, 1258, 1282, 1553, 1605
BmeT110I	CYCGRG	3 cut(s)	541, 1182, 1235
BmgT120I	GGNCC	6 cut(s)	55, 95, 1258, 1282, 1553, 1605
BmiI	GGNNCC	6 cut(s)	56, 257, 859, 1074, 1283, 1545
BmrFI	CCNGG	5 cut(s)	528, 1001, 1168, 1534, 1548
BmsI	GCATC	4 cut(s)	97, 403, 1458, 1585
BmtI	GCTAGC	1 cut(s)	963
BplI	GAGNNNNNCTC	2 cut(s)	678, 710
BpmI	CTGGAG	3 cut(s)	124, 466, 1569
Bpu10I	CCTNAGC	1 cut(s)	251
Bpu14I	TTCGAA	1 cut(s)	1506
BpuEI	CTTGAG	2 cut(s)	86, 827
BpuMI	CCSGG	1 cut(s)	528
Bsa29I	ATCGAT	1 cut(s)	1702
BsaBI	GATNNNNATC	1 cut(s)	1230
BsaI	GGTCTC	1 cut(s)	587
BsaJI	CCNNGG	4 cut(s)	999, 1000, 1167, 1261
BsaWI	WCCGGW	2 cut(s)	698, 1678
Bsc4I	CCNNNNNNNGG	4 cut(s)	173, 771, 1195, 1291
Bse118I	RCCGGY	2 cut(s)	284, 1678
Bse1I	ACTGG	1 cut(s)	107
Bse21I	CCTNAGG	1 cut(s)	1142
Bse3DI	GCAATG	3 cut(s)	303, 776, 1227
Bse8I	GATNNNNATC	1 cut(s)	1230
BseAI	TCCGGA	1 cut(s)	698
BseBI	CCWGG	4 cut(s)	1001, 1168, 1534, 1548
BseCI	ATCGAT	1 cut(s)	1702
BseDI	CCNNGG	4 cut(s)	999, 1000, 1167, 1261
BseGI	GGATG	4 cut(s)	898, 919, 951, 1600
BseJI	GATNNNNATC	1 cut(s)	1230
BseLI	CCNNNNNNNGG	4 cut(s)	173, 771, 1195, 1291
BseMI	GCAATG	3 cut(s)	303, 776, 1227
BseMII	CTCAG	4 cut(s)	56, 242, 620, 1156
BseNI	ACTGG	1 cut(s)	107
BsePI	GCGCGC	1 cut(s)	634
BseRI	GAGGAG	2 cut(s)	268, 1322
BseSI	GKGCMC	3 cut(s)	559, 1650, 1678
BseXI	GCAGC	6 cut(s)	300, 360, 817, 1204, 1393, 1581
BsgI	GTGCAG	1 cut(s)	1109
Bsh1236I	CGCG	7 cut(s)	76, 387, 411, 636, 905, 985, 1098
Bsh1285I	CGRYCG	1 cut(s)	909
BshFI	GGCC	6 cut(s)	47, 155, 764, 1392, 1484, 1532
BshNI	GGYRCC	2 cut(s)	1072, 1543
BshTI	ACCGGT	1 cut(s)	1678
BshVI	ATCGAT	1 cut(s)	1702
BsiEI	CGRYCG	1 cut(s)	909
BsiHKAI	GWGCWC	3 cut(s)	559, 1650, 1678
BsiHKCI	CYCGRG	3 cut(s)	541, 1182, 1235
BsiSI	CCGG	4 cut(s)	285, 528, 699, 1679
BslFI	GGGAC	2 cut(s)	982, 1172
BslI	CCNNNNNNNGG	4 cut(s)	173, 771, 1195, 1291
BsmAI	GTCTC	1 cut(s)	587
BsmFI	GGGAC	2 cut(s)	982, 1172
BsmI	GAATGC	1 cut(s)	1444
BsnI	GGCC	6 cut(s)	47, 155, 764, 1392, 1484, 1532
Bso31I	GGTCTC	1 cut(s)	587
BsoBI	CYCGRG	3 cut(s)	541, 1182, 1235
Bsp119I	TTCGAA	1 cut(s)	1506
Bsp1286I	GDGCHC	4 cut(s)	559, 815, 1650, 1678
Bsp13I	TCCGGA	1 cut(s)	698
Bsp1407I	TGTACA	1 cut(s)	1338
Bsp68I	TCGCGA	1 cut(s)	411
BspACI	CCGC	5 cut(s)	385, 620, 761, 1096, 1689
BspANI	GGCC	6 cut(s)	47, 155, 764, 1392, 1484, 1532
BspCNI	CTCAG	4 cut(s)	55, 243, 621, 1155
BspDI	ATCGAT	1 cut(s)	1702
BspEI	TCCGGA	1 cut(s)	698
BspFNI	CGCG	7 cut(s)	76, 387, 411, 636, 905, 985, 1098
BspLI	GGNNCC	6 cut(s)	56, 257, 859, 1074, 1283, 1545
BspMAI	CTGCAG	2 cut(s)	1109, 1571
BspMI	ACCTGC	2 cut(s)	69, 1112
BspOI	GCTAGC	1 cut(s)	963
BspPI	GGATC	3 cut(s)	611, 1039, 1226
BspQI	GCTCTTC	1 cut(s)	240
BspT104I	TTCGAA	1 cut(s)	1506
BspT107I	GGYRCC	2 cut(s)	1072, 1543
BspTI	CTTAAG	1 cut(s)	560
BspTNI	GGTCTC	1 cut(s)	587
BsrDI	GCAATG	3 cut(s)	303, 776, 1227
BsrFI	RCCGGY	2 cut(s)	284, 1678
BsrGI	TGTACA	1 cut(s)	1338
BsrI	ACTGG	1 cut(s)	107
BssAI	RCCGGY	2 cut(s)	284, 1678
BssECI	CCNNGG	4 cut(s)	999, 1000, 1167, 1261
BssHII	GCGCGC	1 cut(s)	634
BssSI	CACGAG	1 cut(s)	869
BssT1I	CCWWGG	1 cut(s)	1261
Bst2BI	CACGAG	1 cut(s)	869
Bst2UI	CCWGG	4 cut(s)	1001, 1168, 1534, 1548
Bst4CI	ACNGT	4 cut(s)	220, 862, 886, 1543
Bst6I	CTCTTC	3 cut(s)	240, 315, 1080
BstAFI	CTTAAG	1 cut(s)	560
BstAPI	GCANNNNNTGC	1 cut(s)	1595
BstAUI	TGTACA	1 cut(s)	1338
BstBI	TTCGAA	1 cut(s)	1506
BstC8I	GCNNGC	6 cut(s)	153, 409, 636, 751, 961, 1567
BstDEI	CTNAG	6 cut(s)	42, 251, 629, 1142, 1174, 1721
BstENI	CCTNNNNNAGG	1 cut(s)	1289
BstF5I	GGATG	4 cut(s)	898, 919, 951, 1600
BstFNI	CGCG	7 cut(s)	76, 387, 411, 636, 905, 985, 1098
BstH2I	RGCGCY	1 cut(s)	611
BstHHI	GCGC	5 cut(s)	78, 610, 636, 638, 779
BstMAI	GTCTC	1 cut(s)	587
BstMCI	CGRYCG	1 cut(s)	909
BstMWI	GCNNNNNNNGC	6 cut(s)	118, 393, 413, 1104, 1351, 1595
BstNI	CCWGG	4 cut(s)	1001, 1168, 1534, 1548
BstNSI	RCATGY	2 cut(s)	361, 1132
BstSCI	CCNGG	5 cut(s)	526, 999, 1166, 1532, 1546
BstSFI	CTRYAG	3 cut(s)	1105, 1375, 1567
BstSLI	GKGCMC	3 cut(s)	559, 1650, 1678
BstUI	CGCG	7 cut(s)	76, 387, 411, 636, 905, 985, 1098
BstV1I	GCAGC	6 cut(s)	300, 360, 817, 1204, 1393, 1581
BstX2I	RGATCY	1 cut(s)	1031
BstXI	CCANNNNNNTGG	1 cut(s)	1585
BstYI	RGATCY	1 cut(s)	1031
Bsu15I	ATCGAT	1 cut(s)	1702
Bsu36I	CCTNAGG	1 cut(s)	1142
BsuI	GTATCC	2 cut(s)	373, 1046
BsuRI	GGCC	6 cut(s)	47, 155, 764, 1392, 1484, 1532
BsuTUI	ATCGAT	1 cut(s)	1702
BtgZI	GCGATG	1 cut(s)	426
BtsCI	GGATG	4 cut(s)	898, 919, 951, 1600
BtsI	GCAGTG	2 cut(s)	732, 1121
BtsIMutI	CAGTG	3 cut(s)	732, 1121, 1419
BtuMI	TCGCGA	1 cut(s)	411
BveI	ACCTGC	2 cut(s)	69, 1112
Cac8I	GCNNGC	6 cut(s)	153, 409, 636, 751, 961, 1567
CaiI	CAGNNNCTG	1 cut(s)	151
CfoI	GCGC	5 cut(s)	78, 610, 636, 638, 779
Cfr10I	RCCGGY	2 cut(s)	284, 1678
Cfr13I	GGNCC	6 cut(s)	55, 95, 1258, 1282, 1553, 1605
ClaI	ATCGAT	1 cut(s)	1702
CseI	GACGC	1 cut(s)	911
Csp6I	GTAC	6 cut(s)	115, 538, 571, 1060, 1339, 1717
CspAI	ACCGGT	1 cut(s)	1678
CviAII	CATG	8 cut(s)	122, 242, 358, 979, 1129, 1403, 1415, 1590
CviQI	GTAC	6 cut(s)	115, 538, 571, 1060, 1339, 1717
DdeI	CTNAG	6 cut(s)	42, 251, 629, 1142, 1174, 1721
DraI	TTTAAA	1 cut(s)	1243
EaeI	YGGCCR	3 cut(s)	153, 762, 1530
Eam1104I	CTCTTC	3 cut(s)	240, 315, 1080
EarI	CTCTTC	3 cut(s)	240, 315, 1080
Eco130I	CCWWGG	1 cut(s)	1261
Eco147I	AGGCCT	1 cut(s)	1484
Eco24I	GRGCYC	1 cut(s)	815
Eco31I	GGTCTC	1 cut(s)	587
Eco32I	GATATC	1 cut(s)	471
Eco47I	GGWCC	6 cut(s)	55, 95, 1258, 1282, 1553, 1605
Eco57I	CTGAAG	1 cut(s)	845
Eco81I	CCTNAGG	1 cut(s)	1142
Eco88I	CYCGRG	3 cut(s)	541, 1182, 1235
EcoNI	CCTNNNNNAGG	1 cut(s)	1289
EcoO109I	RGGNCCY	1 cut(s)	1282
EcoRI	GAATTC	2 cut(s)	9, 1692
EcoRII	CCWGG	4 cut(s)	999, 1166, 1532, 1546
EcoRV	GATATC	1 cut(s)	471
EcoT14I	CCWWGG	1 cut(s)	1261
EcoT38I	GRGCYC	1 cut(s)	815
ErhI	CCWWGG	1 cut(s)	1261
FaeI	CATG	8 cut(s)	125, 245, 361, 982, 1132, 1406, 1418, 1593
FalI	AAGNNNNNCTT	2 cut(s)	543, 575
FaqI	GGGAC	2 cut(s)	982, 1172
FatI	CATG	8 cut(s)	121, 241, 357, 978, 1128, 1402, 1414, 1589
FauI	CCCGC	1 cut(s)	392
FauNDI	CATATG	2 cut(s)	613, 1660
FbaI	TGATCA	3 cut(s)	178, 654, 1248
Fnu4HI	GCNGC	7 cut(s)	289, 374, 762, 831, 1218, 1407, 1570
FokI	GGATG	4 cut(s)	905, 926, 938, 1607
FriOI	GRGCYC	1 cut(s)	815
Fsp4HI	GCNGC	7 cut(s)	289, 374, 762, 831, 1218, 1407, 1570
FspBI	CTAG	2 cut(s)	960, 1686
GlaI	GCGC	5 cut(s)	77, 609, 635, 637, 778
GluI	GCNGC	7 cut(s)	289, 374, 762, 831, 1218, 1407, 1570
GsuI	CTGGAG	3 cut(s)	124, 466, 1569
HaeII	RGCGCY	1 cut(s)	611
HaeIII	GGCC	6 cut(s)	47, 155, 764, 1392, 1484, 1532
HapII	CCGG	4 cut(s)	285, 528, 699, 1679
HgaI	GACGC	1 cut(s)	911
HhaI	GCGC	5 cut(s)	78, 610, 636, 638, 779
Hin1II	CATG	8 cut(s)	125, 245, 361, 982, 1132, 1406, 1418, 1593
Hin6I	GCGC	5 cut(s)	76, 608, 634, 636, 777
HinP1I	GCGC	5 cut(s)	76, 608, 634, 636, 777
HinfI	GANTC	7 cut(s)	38, 268, 423, 448, 710, 1185, 1512
HpaII	CCGG	4 cut(s)	285, 528, 699, 1679
HphI	GGTGA	7 cut(s)	188, 232, 807, 857, 1027, 1037, 1693
Hpy166II	GTNNAC	5 cut(s)	95, 557, 865, 1648, 1676
Hpy8I	GTNNAC	5 cut(s)	95, 557, 865, 1648, 1676
Hpy99I	CGWCG	1 cut(s)	1367
HpyAV	CCTTC	5 cut(s)	545, 802, 820, 1249, 1342
HpyCH4III	ACNGT	4 cut(s)	220, 862, 886, 1543
HpyCH4V	TGCA	9 cut(s)	557, 833, 845, 1107, 1126, 1220, 1569, 1648, 1676
HpyF10VI	GCNNNNNNNGC	6 cut(s)	118, 393, 413, 1104, 1351, 1595
HpyF3I	CTNAG	6 cut(s)	42, 251, 629, 1142, 1174, 1721
Hsp92II	CATG	8 cut(s)	125, 245, 361, 982, 1132, 1406, 1418, 1593
HspAI	GCGC	5 cut(s)	76, 608, 634, 636, 777
Kpn2I	TCCGGA	1 cut(s)	698
Ksp22I	TGATCA	3 cut(s)	178, 654, 1248
LguI	GCTCTTC	1 cut(s)	240
LmnI	GCTCC	4 cut(s)	255, 789, 1309, 1351
Lsp1109I	GCAGC	6 cut(s)	300, 360, 817, 1204, 1393, 1581
LweI	GCATC	4 cut(s)	97, 403, 1458, 1585
MaeI	CTAG	2 cut(s)	960, 1686
MaeIII	GTNAC	5 cut(s)	214, 740, 943, 1417, 1681
MboII	GAAGA	7 cut(s)	257, 332, 683, 1097, 1278, 1444, 1507
MfeI	CAATTG	1 cut(s)	207
MflI	RGATCY	1 cut(s)	1031
MhlI	GDGCHC	4 cut(s)	559, 815, 1650, 1678
MlsI	TGGCCA	1 cut(s)	155
MluCI	AATT	6 cut(s)	9, 207, 897, 1041, 1269, 1692
MluNI	TGGCCA	1 cut(s)	155
MlyI	GAGTC	3 cut(s)	32, 457, 1194
MmeI	TCCRAC	2 cut(s)	781, 1361
Mox20I	TGGCCA	1 cut(s)	155
MroI	TCCGGA	1 cut(s)	698
MroXI	GAANNNNTTC	1 cut(s)	427
MscI	TGGCCA	1 cut(s)	155
MseI	TTAA	3 cut(s)	561, 1008, 1242
MslI	CAYNNNNRTG	4 cut(s)	315, 968, 1131, 1583
Msp20I	TGGCCA	1 cut(s)	155
MspA1I	CMGCKG	1 cut(s)	1583
MspCI	CTTAAG	1 cut(s)	560
MspI	CCGG	4 cut(s)	285, 528, 699, 1679
MspR9I	CCNGG	5 cut(s)	528, 1001, 1168, 1534, 1548
MunI	CAATTG	1 cut(s)	207
Mva1269I	GAATGC	1 cut(s)	1444
MvaI	CCWGG	4 cut(s)	1001, 1168, 1534, 1548
MvnI	CGCG	7 cut(s)	76, 387, 411, 636, 905, 985, 1098
MwoI	GCNNNNNNNGC	6 cut(s)	118, 393, 413, 1104, 1351, 1595
NciI	CCSGG	1 cut(s)	528
NdeI	CATATG	2 cut(s)	613, 1660
NheI	GCTAGC	1 cut(s)	959
NlaIII	CATG	8 cut(s)	125, 245, 361, 982, 1132, 1406, 1418, 1593
NlaIV	GGNNCC	6 cut(s)	56, 257, 859, 1074, 1283, 1545
NmeAIII	GCCGAG	1 cut(s)	1508
NmuCI	GTSAC	4 cut(s)	214, 740, 1417, 1681
NruI	TCGCGA	1 cut(s)	411
NspI	RCATGY	2 cut(s)	361, 1132
NspV	TTCGAA	1 cut(s)	1506
OliI	CACNNNNGTG	1 cut(s)	1583
PaeR7I	CTCGAG	1 cut(s)	541
PalAI	GGCGCGCC	1 cut(s)	634
PasI	CCCWGGG	1 cut(s)	1000
PauI	GCGCGC	1 cut(s)	634
PceI	AGGCCT	1 cut(s)	1484
PciI	ACATGT	1 cut(s)	1128
PciSI	GCTCTTC	1 cut(s)	240
PcsI	WCGNNNNNNNCGW	1 cut(s)	408
PctI	GAATGC	1 cut(s)	1444
PdmI	GAANNNNTTC	1 cut(s)	427
PfeI	GAWTC	4 cut(s)	268, 423, 710, 1512
PflFI	GACNNNGTC	1 cut(s)	1367
PflMI	CCANNNNNTGG	1 cut(s)	771
PinAI	ACCGGT	1 cut(s)	1678
PkrI	GCNGC	7 cut(s)	290, 375, 763, 832, 1219, 1408, 1571
Ple19I	CGATCG	1 cut(s)	909
PleI	GAGTC	3 cut(s)	32, 456, 1193
PpsI	GAGTC	3 cut(s)	32, 456, 1193
PpuMI	RGGWCCY	1 cut(s)	1282
PscI	ACATGT	1 cut(s)	1128
Psp5II	RGGWCCY	1 cut(s)	1282
Psp6I	CCWGG	4 cut(s)	999, 1166, 1532, 1546
PspGI	CCWGG	4 cut(s)	999, 1166, 1532, 1546
PspN4I	GGNNCC	6 cut(s)	56, 257, 859, 1074, 1283, 1545
PspPI	GGNCC	6 cut(s)	55, 95, 1258, 1282, 1553, 1605
PspPPI	RGGWCCY	1 cut(s)	1282
PspXI	VCTCGAGB	1 cut(s)	541
PstI	CTGCAG	2 cut(s)	1109, 1571
PstNI	CAGNNNCTG	1 cut(s)	151
PsuI	RGATCY	1 cut(s)	1031
PsyI	GACNNNGTC	1 cut(s)	1367
PteI	GCGCGC	1 cut(s)	634
PvuI	CGATCG	1 cut(s)	909
PvuII	CAGCTG	1 cut(s)	1583
RruI	TCGCGA	1 cut(s)	411
RsaI	GTAC	6 cut(s)	116, 539, 572, 1061, 1340, 1718
RsaNI	GTAC	6 cut(s)	115, 538, 571, 1060, 1339, 1717
RseI	CAYNNNNRTG	4 cut(s)	315, 968, 1131, 1583
SapI	GCTCTTC	1 cut(s)	240
SaqAI	TTAA	3 cut(s)	561, 1008, 1242
SatI	GCNGC	7 cut(s)	289, 374, 762, 831, 1218, 1407, 1570
Sau96I	GGNCC	6 cut(s)	55, 95, 1258, 1282, 1553, 1605
SchI	GAGTC	3 cut(s)	32, 457, 1194
ScrFI	CCNGG	5 cut(s)	528, 1001, 1168, 1534, 1548
SduI	GDGCHC	4 cut(s)	559, 815, 1650, 1678
SfaNI	GCATC	4 cut(s)	97, 403, 1458, 1585
SfcI	CTRYAG	3 cut(s)	1105, 1375, 1567
Sfr274I	CTCGAG	1 cut(s)	541
SfuI	TTCGAA	1 cut(s)	1506
SgrAI	CRCCGGYG	1 cut(s)	1678
SgsI	GGCGCGCC	1 cut(s)	634
SinI	GGWCC	6 cut(s)	55, 95, 1258, 1282, 1553, 1605
SlaI	CTCGAG	1 cut(s)	541
SmiMI	CAYNNNNRTG	4 cut(s)	315, 968, 1131, 1583
SmlI	CTYRAG	4 cut(s)	65, 541, 560, 806
SmoI	CTYRAG	4 cut(s)	65, 541, 560, 806
Sse9I	AATT	6 cut(s)	9, 207, 897, 1041, 1269, 1692
SseBI	AGGCCT	1 cut(s)	1484
SsiI	CCGC	5 cut(s)	385, 620, 761, 1096, 1689
SspMI	CTAG	2 cut(s)	960, 1686
StuI	AGGCCT	1 cut(s)	1484
StyD4I	CCNGG	5 cut(s)	526, 999, 1166, 1532, 1546
StyI	CCWWGG	1 cut(s)	1261
TaaI	ACNGT	4 cut(s)	220, 862, 886, 1543
TaqI	TCGA	4 cut(s)	542, 909, 1506, 1702
TaqII	GACCGA	1 cut(s)	1570
TasI	AATT	6 cut(s)	9, 207, 897, 1041, 1269, 1692
TatI	WGTACW	3 cut(s)	537, 1059, 1338
TauI	GCSGC	1 cut(s)	764
TfiI	GAWTC	4 cut(s)	268, 423, 710, 1512
Tru1I	TTAA	3 cut(s)	561, 1008, 1242
Tru9I	TTAA	3 cut(s)	561, 1008, 1242
TscAI	CASTG	3 cut(s)	739, 1128, 1426
TseFI	GTSAC	4 cut(s)	214, 740, 1417, 1681
TseI	GCWGC	6 cut(s)	288, 373, 830, 1217, 1406, 1569
Tsp45I	GTSAC	4 cut(s)	214, 740, 1417, 1681
TspDTI	ATGAA	7 cut(s)	258, 333, 388, 702, 866, 1053, 1451
TspRI	CASTG	3 cut(s)	739, 1128, 1426
Tth111I	GACNNNGTC	1 cut(s)	1367
Van91I	CCANNNNNTGG	1 cut(s)	771
Vha464I	CTTAAG	1 cut(s)	560
VneI	GTGCAC	3 cut(s)	555, 1646, 1674
VpaK11BI	GGWCC	6 cut(s)	55, 95, 1258, 1282, 1553, 1605
XagI	CCTNNNNNAGG	1 cut(s)	1289
XapI	RAATTY	3 cut(s)	9, 1041, 1692
XceI	RCATGY	2 cut(s)	361, 1132
XhoI	CTCGAG	1 cut(s)	541
XmnI	GAANNNNTTC	1 cut(s)	427
XspI	CTAG	2 cut(s)	960, 1686

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.