FvH4_3g39950

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0011193
Physical Location & Seq
Reverse (-)
33822657 .. 33834798
12142 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g39950.t1

Sequence Viewer

Length: 5709 bp
ATGGGGAAGCAGAAGGGAGACGGAGCTAGGAGCAAGGCCCGGCCTTCCAGTAGCAGTTTGGCGGCGTCGCTGTTGCCTTCGGGTTCTACTGCGGCCGTGGGCTTCGGTGGCTACGTCGGAGGCTCTCGCCTCGACGCTCCTCCTTCCGGCGGCGACGATTCACGGCCCTATTTGGAAGTAGATAGTGATTTGGCTCTACATTTGAAGAGGCTTGCAAGGAAAGATCCCACCACCAAGCTTAAAGCTTTGGCGTCTCTATCCACGCTGCTGAAGGAAAAGTCCACAAAGGACATAATACCAGCCATTCCACAATGGGGATTTGAATACAAAAGGCTTGTGGTGGACTACAATAGGGATGTGCGGCGAGCAACTCATGACACAATGAACAATCTTGTCACCGCCGTTGGGAGAGATTTAGCTCCACAGCTCAAATCTCTGATGGGACCATGGTGGTTTTCTCAATTTGACCCAGTATCTGAAGTTTCACAAGCTGCAAAGCGATCATTTCAGGCAGTGTTTTCAGCTCCAGAAAAGAGACTAGATGCTTTAATTTTATGCACGGCAGAGATTTTTGTTTATTTGGAAGAAAATCTGAGGCTCACGCCAGAAAGTATGTCCGATAAAGGAACTGCTCTGGATGAATTACAAGAGATGCATCAGCAGGTGATATCATCATCATTGCTGGCATTGGCCACACTTCTTGATGTCTTGGTTTGCTTACAAGTAGAAAGACCAGGCACCGTAAACATAGCTGCTCAACCAAAACATGCTTTGAAGGCTAGGGAAACTGCTATTTCTTGTGCCGAAAAGATGTTCACTGCTCATAGATTTTTTTTAGACTTCCTGAAATCCCCAAGTCCAGCTATCCGATCAGCCACATATTATGTATTGAGTAGTTTCATAAAAAATGTCCCTCAAGCCTTTAATGAAGGAAATATGAAAACTCTTGCTGCTGCACTATTAGGTGGATTTCAGGAGAAGGATCCTGCTTGTCATTCATCAATGTGGGATGCAATTTTACTGTTTTCGAGTAAATTTCCTGAAAGTTGGACTTCGGTCAATGTACAGAAAGCTGTACTGAATCGGTTTTGGGATTTCCTTAGAAATAGGTGCTTTGGATCTCAACAGGTTTCTTATCCATCTTTGATTCTATTCTTACAAACCGTGCCATCTAAAGCGGTAGTTGCAGAAACTTTCTTTCTTGAATTTTTCAAGAACCTCTGGGCGGGAAGAAATCCATCACATTCCTTGGATGCAGATAGAGTAGCATATTTTCAAGCGTTTCAAGAATGCTTTCTTTGGGCATTGCATAATGCGTCAAGATATTGCAATGGAGTGGATTCCATCAGTGCTTTTCGAGCAACTCTTGTTAAAAGTGTTCTGGTAAAGCTATTGTGGCAAGACTACATTTCCTCTAGTAGCTCAAGAAAGAAGGAGAAAACATCGCTGGGATTATCAGCAGATTCTTGTGAAAGTGATTTAACTTCGAACAAGAAGACTGTTGAAACTTTGAATATCACGTACCCAATGAGCTACTTTAATGAGTTGGCAAACTGCATTGTTGCGGTTCTTTCAGGCATTCATTTGCTGGAGCATGATTTGCTCTCAGTTTTCGCTGCAGAATTTCAGGAAAACTGTCGTGGTTTCTTTCAGCATGCTAGTAACTTGGAAAAAGAAAGTGAGTTTGCAGAACGAGTTACACAGTTTATATCATTACTTGGGGAATGTTCAATGCAGAATGGTGGAGGTTGGCCTTTGGCTTCTCTAGTTGGACCAATGTTGGCTAATTCCTTTGCAGTGATGAGATCACATGATTCACCAAGTTGTGTGAAGATTCTAGCACAGTCTGTTTCTGTATTTGGGCCACATAAGATAATCCACGAACTTCGTATTCACAATATGTCTCCACATGAGGGTGATACAGCATTAGAGGAAGAAACATTCCTGCAAATGTTCAAGGGAACATTTGTTCCATGGTGTCTCAGTGGGAATAGTTGCTCTTTGAGTGCTCGTCTTGACCTGTTACTTGCATTGCTTGATGATGAATATTTCTTTGAGCAGTGGGATAGTGTTATCAGATATGCTACTAACCTGGAGTATTCTGGATCTGCACCCTGCTCTTTGGATTCTGACCGGATAACTATCTTAGCAATGCTTTTAGAGAAAGCAAGAAATGAAATCACAAAAGCGAAAGTAGGAATTTCTATCTGCACGAACATGGGCAACATAGATCATTGGCATCACGAGCTTCTAGAGTCAACTGTGGTTGCTGTTGCTCGATCCTCACCTCCTTTCGGAGCTTCTAGTTCTCAATTTTTGTGTACTGTTGTTGGTGGTCCAACAAAGAGCAATCAAATTTCTCTGGTGTCAAGAAATACGTTGGTCCTGATATTTGAAGAGGTTTTCAAGAAGTTACTTTCATTTATTCTGGCATCCTCGTTTACTTGGGTCAGGGATGCAGGTTCTCTATTGACGCCCAATCTATTGACTGCTGGGGCAAACACCATTGGGTCTGAATTTGAAAGTTCAGTGAGCATGTTTGAGATGGCTCAATTTGCTCTTGAAGTACTTGATGGCGGCTTATATTCATTGAAGACACTTGGTGAAGAAAGTGGGCTGACTCCAGCTATTTTAGCTGCAATTTTTCTTATTGATTGGGAGTTTCTGGAACTGACAATGATAGATGATGGACCTGATGATAAATCAAAGGAAATATTGAAGGCCAGGCTGGGATTTGGTGAATCTTTCCATGCATTTAGATGTAAGTTAGGTAATCAATTCTGGAAAACACTCAGTTTACACAATCGAAAGGCACTTGGACAAAAATTGATTCAGTGCATGAGATCCGCCATCTTCAATGAAGAGGAAATGGACACAGAGAAGTTCACGTCACTGTGCTGCTTGTGGATGCTTGAAATTCTGGACTGTCTCTCTGAGGATCCCTTTGAGGAACAGGATCTGCTGGACCGACTTCTCTGCCAAGGTGAGAGATGGCCTTTGTGGATAGTTCCGGAATTTAGCAGGCAAGAGGGAACAGTTGCCAAAGACTTCTCTATCCAAGACTTTGGCCATCGGAAGTTTATTTCATTTATTGACAAGATGATATCAGAAATTGGGATTGATAGAGTTGTTGCCAGTTGTGGTAGAAATGCTCTCCCTCTGTCTGAGGAAGCAACAAATGAAAACCTGACTCGATCTTGGCTTGCTGCTGAAATATTATGCTCCTGGAAGTGGCCAGGAGGAAGTGTAGTAGCTTCATTCTTACCTTCATTAAGCGCGTATGCCAAGAGTAAAAATTTCTCTTCCCAGGAGAGCTTGCTAGATTCTATTTTCAACATTTTACTTGATGGTACACTTGTTCAGGGAGGATGTGCTGCACAGAATTTCGTCTATTTATGTGCTGCTTCAAGTGATGAGGTGGAGGATATTGAAGAACCATTCTTAAGAGCTCTTGTAGCTTTTCTTTTAACTTTGTTTAATGATAATATATGGGGTTACAAGAAAGCTATGGAGCTGTTTGCACTGCTTGTAAATAAACTTTATGTTGGAGAAGCAACAAATGCAAACTGTTTGAGGATTCTTCCTGTGATCGTCAATGCTCTTATACTACCATTAAGTCAAAGAAGCATCAGATCTAATGACTCTAGTGGAGATGCTCAACATGATTCTTCTGGGGAAAATCATATACACGATGTTATTGAAGGCTGGCTTAGAAAAGCTCTATCATTTCCACCTTTGATTACGTGGCAAACAGGAGAAGATATGGAAGATTGGATGCAGCTGGTTATTTCTTGTTATCCCTTTAGTGTAGTGGAAGGCATACAAACACCAAAGCTGGAGAGAAGGATTAGCTTGGTAGAGAGAAAGCTCCTACTTGAATTGTTTCGGAAGCAGAGACATGGTGTTGGAACATCAGCTGTAATTAATCAGCTTCCAGTTGTTCAGATGTTGCTATCAAAGCTTATGGTCGTTTCAGTTGGTTATTGTTGGAAAGAATTTGATGAAGAAGACTGGGAGTTTGTCTTATCTCAGATAAGACGCTGGCTGCAGACTGTCGTTGTAATGATGGAGGAAATTGCTGAAAATGTCAACGACACTATCACTAGCAGCTTTACTTCTGATAACTTGGATGCTCTCATTGATAACCTTGGCAAAATTGTTTTCGTTTCAGATCCCTTTCCTATGGACATTGCCAAAAATGCCCTTCTATCATTTTCTCTGTCATGTGGATCTTTTGGTCGCCAACAGGCAGAAGATGCAGATAACTTGAATCCTGTGAGAACAGAAAGATGGGACCCCATCAAAAATAGAATTTTAGAAGGCATCCTTAGACTGTTTTTCTGCACGGGTATTGCTGAGGCCATTGCAAGCTCCTGTTGTCATGAGGCTGCATTTATTGTATCCGCATCCCGATTTGAACATTCCTATTTCTGGGAGTTGGTGGCCTCAAGTGTTGTCAACTCGTCAACAGATGCCATAGATAGGGCAGTGAAGTCAGTAGAATTCTGGGGGCTAAGCAAAGGACCCATAAGCTCTTTGTATGCTATCCTCTTTTCTGCCAAATCAGTTCCTTTGTTGCAGTTTTCTGCTTACTTTATTCTTTCAACTGAACTTGTTTTACCCCTGGCTATTGTTGAAGAAGACAAAAGTTACTTGGATGGTGTCAGTAACAATGAAGAGGTGTTATCCCCTCCTGACATGTCAACAGAAACGGATATTCATTTGAGAGCTGAAATATCTTGCATGATTGAAAAGTTGCCTAGTAATGTTCTCGAGATGGATTTGCTGGCAGATCAAAGGGTACATGTTTTCCTTGCTTGGTCCTTGTTGTTATCACATTTAGGGTCATTACCATCGTCATCTCCTACGAGGGAGAGACTGGTCCAGTATGTACAAGATTCTGCTAGTTCAGTAATATTAGATTGCCTCTTCCAGCATATTCCTCTAGAACAGTGGATTCTTAAGAAGAAAGATGAAGAACTTCCTGCTGGTATAGCAGAAGCTGCAGCTTCAGCAACACGTTCCATTAGAACTGGTTCATTATTGTTTGCTGTACAATCTCTCTGGCCTGTTAAACCACTAAAAATGGCTTCACTTGCTGGAGCAATGTTTGGTCGGATGCTCCACATTCTTCCCGCCTATGTAAGACAGTGGTCCAATGATTTACGTGACCGCTCAACATTATCAGGAATTGAATCATTCACAAGAGCATGGTGCAGCCCACATCTAATCGCTGGCGAATTAAGCCAGATTAAAAAGGATGAAATTGCTGACGAGAATTTCACCATTGCTGTAAGCAAATCAGCAAATGAGGTTGTTGCTACTTATACAAAGGATGAAACTGCAATGAATCTTGTAATTCGTCTTCCTTCATCTTACCCATTACGGCCAGTTGATGTTGACTGTACAAGGAGTCTAGGTATTAGTGAAGCAAAACAGAGGAAATGGTCAATGTCTATGACATCATTCGTGCGGAATCAGAATGGAGCTTTAGCAGAAGCTATTCGGATATGGAAGCGCAACTTTGACAAGGAGTTTGAAGGTGTTGAGGAGTGTCCTATATGTTACAGTGTCATCCATACTGTCAACCACGCCCTTCCTCGTCTTGCATGCAAAACCTGCAAGCACAAGTTCCACTCGGCCTGCCTCTACAAGTGGTTCTCAACTTCTCATAAATCAACTTGCCCATTGTGCCAGTCTCCATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

1903

Amino Acids

211.98

Weight (kDa)

5.5

Isoelectric Point (pI)

50.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ltn1_1st PF22958 61 - 388 1.2e-90 E3 ubiquitin-protein ligase listerin, N-terminal domain
LTN1_E3_ligase_6th PF22999 1509 - 1752 8.3e-28 LTN1 family HEAT repeat region
UBC_like PF23009 1762 - 1845 8.7e-30 Ubiquitin conjugating domain-like
zf-RING_2 PF13639 1851 - 1899 7.6e-07 Ring finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G58410 AT5G58410
fragaria_vesca FvH4_3g39950 FvH4_3g39950 FvH4_3g39950
malus_domestica MD03G1058100.v1.1
prunus_persica Prupe.6G045900_v2.0.a1 Prupe.6G045900_v2.0.a1 Prupe.6G045900_v2.0.a1
pyrus_communis pycom03g04640
rosa_chinensis RchiOBHm_Chr5g0071961
rosa_laevigata RLG00000036266
rosa_multiflora Rmu_sc0012806.1_g000004
rosa_roxburghii Rroxscaffold_1G00009240
rosa_rugosa Rorug05G0415700
rosa_samantha Rh5AG471700 Rh5BG491700 Rh5CG515500 Rh5DG503100
rosa_wichuraiana Rw5G043850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 652
Acc36I ACCTGC 3 cut(s) 652, 2450, 5627
AccB1I GGYRCC 1 cut(s) 737
AccBSI CCGCTC 1 cut(s) 5175
AccII CGCG 1 cut(s) 3278
AccIII TCCGGA 1 cut(s) 3010
AcoI YGGCCR 5 cut(s) 93, 690, 3067, 3233, 5387
AcuI CTGAAG 3 cut(s) 290, 498, 4995
AcyI GRCGYC 3 cut(s) 65, 251, 2474
AdeI CACNNNGTG 1 cut(s) 2603
AflII CTTAAG 2 cut(s) 3442, 4961
AflIII ACRYGT 3 cut(s) 4698, 4804, 5018
AjiI CACGTC 1 cut(s) 2889
AjnI CCWGG 7 cut(s) 733, 2091, 2723, 3224, 3235, 3306, 4623
AloI GAACNNNNNNTCC 4 cut(s) 1953, 1954, 1985, 1986
Alw21I GWGCWC 2 cut(s) 2011, 3451
Alw26I GTCTC 9 cut(s) 12, 258, 529, 1908, 1985, 2933, 3889, 4870, 5703
AlwNI CAGNNNCTG 3 cut(s) 476, 2959, 5003
Ama87I CYCGRG 1 cut(s) 4772
Aor13HI TCCGGA 1 cut(s) 3010
ArsI GACNNNNNNTTYG 4 cut(s) 4001, 4033, 5519, 5551
AseI ATTAAT 1 cut(s) 3924
Asp700I GAANNNNTTC 5 cut(s) 1293, 3882, 4980, 5035, 5502
AspLEI GCGC 2 cut(s) 3278, 5520
AsuC2I CCSGG 1 cut(s) 40
AsuHPI GGTGA 9 cut(s) 388, 676, 1809, 1928, 2277, 2615, 2750, 2996, 5275
AsuII TTCGAA 1 cut(s) 1487
AvaI CYCGRG 1 cut(s) 4772
BaeI ACNNNNGTAYC 2 cut(s) 3342, 3375
BalI TGGCCA 3 cut(s) 692, 3069, 3235
BamHI GGATCC 2 cut(s) 982, 2938
BanI GGYRCC 1 cut(s) 737
BanII GRGCYC 1 cut(s) 3451
BarI GAAGNNNNNNTAC 2 cut(s) 5349, 5381
BauI CACGAG 1 cut(s) 2243
BbsI GAAGAC 5 cut(s) 1502, 2600, 4014, 4647, 5357
Bbv12I GWGCWC 2 cut(s) 2011, 3451
BbvCI CCTCAGC 1 cut(s) 4356
BceAI ACGGC 5 cut(s) 80, 179, 386, 576, 5402
BcgI CGANNNNNNTGC 2 cut(s) 2958, 2992
BciT130I CCWGG 7 cut(s) 735, 2093, 2725, 3226, 3237, 3308, 4625
BciVI GTATCC 1 cut(s) 4411
BcnI CCSGG 1 cut(s) 40
BcoDI GTCTC 9 cut(s) 12, 258, 529, 1908, 1985, 2933, 3889, 4870, 5703
BfmI CTRYAG 3 cut(s) 1617, 4046, 5004
BfrI CTTAAG 2 cut(s) 3442, 4961
BfuAI ACCTGC 3 cut(s) 652, 2450, 5627
BfuI GTATCC 1 cut(s) 4411
BglII AGATCT 1 cut(s) 3632
BlpI GCTNAGC 1 cut(s) 4514
BmcAI AGTACT 1 cut(s) 2568
Bme1390I CCNGG 8 cut(s) 40, 735, 2093, 2725, 3226, 3237, 3308, 4625
BmeT110I CYCGRG 1 cut(s) 4772
BmgBI CACGTC 1 cut(s) 2889
BmiI GGNNCC 7 cut(s) 444, 739, 984, 2940, 4295, 4296, 4525
BmrFI CCNGG 8 cut(s) 40, 735, 2093, 2725, 3226, 3237, 3308, 4625
BmrI ACTGGG 2 cut(s) 464, 4021
BmuI ACTGGG 2 cut(s) 464, 4021
BoxI GACNNNNGTC 2 cut(s) 1055, 5152
BpiI GAAGAC 5 cut(s) 1502, 2600, 4014, 4647, 5357
BpmI CTGGAG 6 cut(s) 510, 1610, 2114, 2607, 3857, 5121
Bpu10I CCTNAGC 1 cut(s) 4356
Bpu1102I GCTNAGC 1 cut(s) 4514
Bpu14I TTCGAA 1 cut(s) 1487
BpuEI CTTGAG 3 cut(s) 900, 1408, 4432
BpuMI CCSGG 1 cut(s) 40
BsaAI YACGTR 3 cut(s) 1521, 3744, 5168
BsaBI GATNNNNATC 1 cut(s) 2141
BsaHI GRCGYC 3 cut(s) 65, 251, 2474
BsaJI CCNNGG 8 cut(s) 96, 446, 1248, 1973, 2980, 3306, 4147, 4623
BsaWI WCCGGW 2 cut(s) 2133, 3010
BsaXI ACNNNNNCTCC 4 cut(s) 3231, 3261, 5404, 5434
Bse8I GATNNNNATC 1 cut(s) 2141
BseAI TCCGGA 1 cut(s) 3010
BseBI CCWGG 7 cut(s) 735, 2093, 2725, 3226, 3237, 3308, 4625
BseDI CCNNGG 8 cut(s) 96, 446, 1248, 1973, 2980, 3306, 4147, 4623
BseJI GATNNNNATC 1 cut(s) 2141
BseMII CTCAG 8 cut(s) 584, 1620, 1996, 2806, 2925, 3156, 4043, 4347
BseRI GAGGAG 2 cut(s) 129, 5564
BseX3I CGGCCG 1 cut(s) 93
BseYI CCCAGC 3 cut(s) 1447, 2492, 2728
BsgI GTGCAG 6 cut(s) 939, 2094, 2194, 3360, 4327, 5236
Bsh1236I CGCG 1 cut(s) 3278
Bsh1285I CGRYCG 1 cut(s) 96
BshNI GGYRCC 1 cut(s) 737
BsiEI CGRYCG 1 cut(s) 96
BsiHKAI GWGCWC 2 cut(s) 2011, 3451
BsiHKCI CYCGRG 1 cut(s) 4772
BsiSI CCGG 4 cut(s) 40, 147, 2134, 3011
BslFI GGGAC 3 cut(s) 456, 896, 4307
BsmAI GTCTC 9 cut(s) 12, 258, 529, 1908, 1985, 2933, 3889, 4870, 5703
BsmBI CGTCTC 2 cut(s) 12, 258
BsmFI GGGAC 3 cut(s) 456, 896, 4307
BsmI GAATGC 2 cut(s) 1295, 1578
BsoBI CYCGRG 1 cut(s) 4772
Bsp119I TTCGAA 1 cut(s) 1487
Bsp1286I GDGCHC 2 cut(s) 2011, 3451
Bsp13I TCCGGA 1 cut(s) 3010
Bsp1407I TGTACA 4 cut(s) 1063, 4891, 5053, 5405
Bsp1720I GCTNAGC 1 cut(s) 4514
Bsp19I CCATGG 2 cut(s) 446, 1973
BspCNI CTCAG 8 cut(s) 585, 1619, 1995, 2805, 2926, 3157, 4042, 4348
BspEI TCCGGA 1 cut(s) 3010
BspFNI CGCG 1 cut(s) 3278
BspHI TCATGA 2 cut(s) 373, 4381
BspLI GGNNCC 7 cut(s) 444, 739, 984, 2940, 4295, 4296, 4525
BspMAI CTGCAG 3 cut(s) 1621, 4050, 5008
BspMI ACCTGC 3 cut(s) 652, 2450, 5627
BspT104I TTCGAA 1 cut(s) 1487
BspT107I GGYRCC 1 cut(s) 737
BspTI CTTAAG 2 cut(s) 3442, 4961
BsrBI CCGCTC 1 cut(s) 5175
BsrGI TGTACA 4 cut(s) 1063, 4891, 5053, 5405
BssECI CCNNGG 8 cut(s) 96, 446, 1248, 1973, 2980, 3306, 4147, 4623
BssNI GRCGYC 3 cut(s) 65, 251, 2474
BssSI CACGAG 1 cut(s) 2243
BssT1I CCWWGG 5 cut(s) 446, 1248, 1973, 2980, 4147
Bst2BI CACGAG 1 cut(s) 2243
Bst2UI CCWGG 7 cut(s) 735, 2093, 2725, 3226, 3237, 3308, 4625
Bst6I CTCTTC 6 cut(s) 200, 2391, 2856, 3307, 4671, 4934
BstACI GRCGYC 3 cut(s) 65, 251, 2474
BstAFI CTTAAG 2 cut(s) 3442, 4961
BstAPI GCANNNNNTGC 5 cut(s) 1600, 3560, 4256, 5003, 5619
BstAUI TGTACA 4 cut(s) 1063, 4891, 5053, 5405
BstBAI YACGTR 3 cut(s) 1521, 3744, 5168
BstBI TTCGAA 1 cut(s) 1487
BstDSI CCRYGG 3 cut(s) 96, 446, 1973
BstFNI CGCG 1 cut(s) 3278
BstHHI GCGC 2 cut(s) 3278, 5520
BstMAI GTCTC 9 cut(s) 12, 258, 529, 1908, 1985, 2933, 3889, 4870, 5703
BstMCI CGRYCG 1 cut(s) 96
BstNI CCWGG 7 cut(s) 735, 2093, 2725, 3226, 3237, 3308, 4625
BstNSI RCATGY 6 cut(s) 770, 1658, 2539, 4702, 4808, 5613
BstPAI GACNNNNGTC 2 cut(s) 1055, 5152
BstSCI CCNGG 8 cut(s) 38, 733, 2091, 2723, 3224, 3235, 3306, 4623
BstSFI CTRYAG 3 cut(s) 1617, 4046, 5004
BstUI CGCG 1 cut(s) 3278
BstV2I GAAGAC 5 cut(s) 1502, 2600, 4014, 4647, 5357
BstXI CCANNNNNNTGG 1 cut(s) 3065
BstZI CGGCCG 1 cut(s) 93
BsuI GTATCC 1 cut(s) 4411
BtgI CCRYGG 3 cut(s) 96, 446, 1973
BtgZI GCGATG 1 cut(s) 1428
BtrI CACGTC 1 cut(s) 2889
BtsI GCAGTG 6 cut(s) 519, 816, 1803, 2066, 3521, 4494
BveI ACCTGC 3 cut(s) 652, 2450, 5627
CaiI CAGNNNCTG 3 cut(s) 476, 2959, 5003
CciI TCATGA 2 cut(s) 373, 4381
CfoI GCGC 2 cut(s) 3278, 5520
CseI GACGC 6 cut(s) 54, 143, 240, 1305, 2482, 4047
CspCI CAANNNNNGTGG 2 cut(s) 682, 717
DraIII CACNNNGTG 1 cut(s) 2603
EaeI YGGCCR 5 cut(s) 93, 690, 3067, 3233, 5387
EagI CGGCCG 1 cut(s) 93
Eam1104I CTCTTC 6 cut(s) 200, 2391, 2856, 3307, 4671, 4934
EarI CTCTTC 6 cut(s) 200, 2391, 2856, 3307, 4671, 4934
EciI GGCGGA 1 cut(s) 2836
Ecl136II GAGCTC 1 cut(s) 3449
EclXI CGGCCG 1 cut(s) 93
Eco130I CCWWGG 5 cut(s) 446, 1248, 1973, 2980, 4147
Eco24I GRGCYC 1 cut(s) 3451
Eco32I GATATC 2 cut(s) 669, 3105
Eco52I CGGCCG 1 cut(s) 93
Eco53kI GAGCTC 1 cut(s) 3449
Eco57I CTGAAG 3 cut(s) 290, 498, 4995
Eco88I CYCGRG 1 cut(s) 4772
EcoICRI GAGCTC 1 cut(s) 3449
EcoO109I RGGNCCY 2 cut(s) 4294, 4523
EcoRI GAATTC 1 cut(s) 4502
EcoRII CCWGG 7 cut(s) 733, 2091, 2723, 3224, 3235, 3306, 4623
EcoRV GATATC 2 cut(s) 669, 3105
EcoT14I CCWWGG 5 cut(s) 446, 1248, 1973, 2980, 4147
EcoT22I ATGCAT 2 cut(s) 657, 2755
EcoT38I GRGCYC 1 cut(s) 3451
ErhI CCWWGG 5 cut(s) 446, 1248, 1973, 2980, 4147
Esp3I CGTCTC 2 cut(s) 12, 258
FalI AAGNNNNNCTT 8 cut(s) 1036, 1068, 3693, 3725, 4311, 4343, 5507, 5539
FaqI GGGAC 3 cut(s) 456, 896, 4307
FauI CCCGC 2 cut(s) 1219, 5143
FriOI GRGCYC 1 cut(s) 3451
GlaI GCGC 2 cut(s) 3277, 5519
GsaI CCCAGC 3 cut(s) 1451, 2496, 2732
GsuI CTGGAG 6 cut(s) 510, 1610, 2114, 2607, 3857, 5121
HapII CCGG 4 cut(s) 40, 147, 2134, 3011
HgaI GACGC 6 cut(s) 54, 143, 240, 1305, 2482, 4047
HhaI GCGC 2 cut(s) 3278, 5520
Hin1I GRCGYC 3 cut(s) 65, 251, 2474
Hin6I GCGC 2 cut(s) 3276, 5518
HinP1I GCGC 2 cut(s) 3276, 5518
HincII GTYRAC 7 cut(s) 2259, 4090, 4459, 4467, 4704, 5401, 5587
HindII GTYRAC 7 cut(s) 2259, 4090, 4459, 4467, 4704, 5401, 5587
HindIII AAGCTT 3 cut(s) 236, 243, 3959
HpaII CCGG 4 cut(s) 40, 147, 2134, 3011
HphI GGTGA 9 cut(s) 388, 676, 1809, 1928, 2277, 2615, 2750, 2996, 5275
Hpy99I CGWCG 4 cut(s) 70, 119, 137, 158
HpyCH4IV ACGT 7 cut(s) 114, 1520, 2378, 2888, 3743, 5020, 5167
HpySE526I ACGT 7 cut(s) 114, 1520, 2378, 2888, 3743, 5020, 5167
Hsp92I GRCGYC 3 cut(s) 65, 251, 2474
HspAI GCGC 2 cut(s) 3276, 5518
KflI GGGWCCC 1 cut(s) 4294
Kpn2I TCCGGA 1 cut(s) 3010
MaeII ACGT 7 cut(s) 114, 1520, 2378, 2888, 3743, 5020, 5167
MbiI CCGCTC 1 cut(s) 5175
MhlI GDGCHC 2 cut(s) 2011, 3451
MlsI TGGCCA 3 cut(s) 692, 3069, 3235
MluNI TGGCCA 3 cut(s) 692, 3069, 3235
MlyI GAGTC 5 cut(s) 2264, 2614, 3184, 3635, 5422
MmeI TCCRAC 8 cut(s) 97, 1028, 1750, 2363, 3526, 3886, 3968, 5096
Mox20I TGGCCA 3 cut(s) 692, 3069, 3235
Mph1103I ATGCAT 2 cut(s) 657, 2755
MroI TCCGGA 1 cut(s) 3010
MroXI GAANNNNTTC 5 cut(s) 1293, 3882, 4980, 5035, 5502
MscI TGGCCA 3 cut(s) 692, 3069, 3235
MslI CAYNNNNRTG 4 cut(s) 1003, 1914, 2216, 2758
Msp20I TGGCCA 3 cut(s) 692, 3069, 3235
MspA1I CMGCKG 2 cut(s) 3781, 3917
MspCI CTTAAG 2 cut(s) 3442, 4961
MspI CCGG 4 cut(s) 40, 147, 2134, 3011
MspR9I CCNGG 8 cut(s) 40, 735, 2093, 2725, 3226, 3237, 3308, 4625
Mva1269I GAATGC 2 cut(s) 1295, 1578
MvaI CCWGG 7 cut(s) 735, 2093, 2725, 3226, 3237, 3308, 4625
MvnI CGCG 1 cut(s) 3278
NciI CCSGG 1 cut(s) 40
NcoI CCATGG 2 cut(s) 446, 1973
NlaIV GGNNCC 7 cut(s) 444, 739, 984, 2940, 4295, 4296, 4525
NmeAIII GCCGAG 1 cut(s) 5618
NmuCI GTSAC 3 cut(s) 394, 2889, 5168
NsiI ATGCAT 2 cut(s) 657, 2755
NspI RCATGY 6 cut(s) 770, 1658, 2539, 4702, 4808, 5613
NspV TTCGAA 1 cut(s) 1487
PaeI GCATGC 2 cut(s) 1658, 5613
PaeR7I CTCGAG 1 cut(s) 4772
PagI TCATGA 2 cut(s) 373, 4381
PaqCI CACCTGC 1 cut(s) 652
PciI ACATGT 2 cut(s) 4698, 4804
PcsI WCGNNNNNNNCGW 1 cut(s) 111
PctI GAATGC 2 cut(s) 1295, 1578
PdmI GAANNNNTTC 5 cut(s) 1293, 3882, 4980, 5035, 5502
PfoI TCCNGGA 1 cut(s) 3224
PleI GAGTC 5 cut(s) 2263, 2614, 3184, 3635, 5421
PpsI GAGTC 5 cut(s) 2263, 2614, 3184, 3635, 5421
Ppu21I YACGTR 3 cut(s) 1521, 3744, 5168
PpuMI RGGWCCY 2 cut(s) 4294, 4523
PscI ACATGT 2 cut(s) 4698, 4804
PshAI GACNNNNGTC 2 cut(s) 1055, 5152
PshBI ATTAAT 1 cut(s) 3924
Psp124BI GAGCTC 1 cut(s) 3451
Psp5II RGGWCCY 2 cut(s) 4294, 4523
Psp6I CCWGG 7 cut(s) 733, 2091, 2723, 3224, 3235, 3306, 4623
PspFI CCCAGC 3 cut(s) 1447, 2492, 2728
PspGI CCWGG 7 cut(s) 733, 2091, 2723, 3224, 3235, 3306, 4623
PspN4I GGNNCC 7 cut(s) 444, 739, 984, 2940, 4295, 4296, 4525
PspPPI RGGWCCY 2 cut(s) 4294, 4523
PsrI GAACNNNNNNTAC 2 cut(s) 4602, 4634
PstI CTGCAG 3 cut(s) 1621, 4050, 5008
PstNI CAGNNNCTG 3 cut(s) 476, 2959, 5003
PvuII CAGCTG 2 cut(s) 3781, 3917
RseI CAYNNNNRTG 4 cut(s) 1003, 1914, 2216, 2758
SacI GAGCTC 1 cut(s) 3451
ScaI AGTACT 1 cut(s) 2568
SchI GAGTC 5 cut(s) 2264, 2614, 3184, 3635, 5422
ScrFI CCNGG 8 cut(s) 40, 735, 2093, 2725, 3226, 3237, 3308, 4625
SduI GDGCHC 2 cut(s) 2011, 3451
SfcI CTRYAG 3 cut(s) 1617, 4046, 5004
Sfr274I CTCGAG 1 cut(s) 4772
SfuI TTCGAA 1 cut(s) 1487
SlaI CTCGAG 1 cut(s) 4772
SmiMI CAYNNNNRTG 4 cut(s) 1003, 1914, 2216, 2758
SmlI CTYRAG 6 cut(s) 915, 1423, 3442, 4447, 4772, 4961
SmoI CTYRAG 6 cut(s) 915, 1423, 3442, 4447, 4772, 4961
SphI GCATGC 2 cut(s) 1658, 5613
SspI AATATT 4 cut(s) 2048, 2715, 3216, 4917
SstI GAGCTC 1 cut(s) 3451
StyD4I CCNGG 8 cut(s) 38, 733, 2091, 2723, 3224, 3235, 3306, 4623
StyI CCWWGG 5 cut(s) 446, 1248, 1973, 2980, 4147
TaiI ACGT 7 cut(s) 117, 1523, 2381, 2891, 3746, 5023, 5170
TaqI TCGA 8 cut(s) 132, 1028, 1357, 1487, 2278, 2806, 3193, 4773
TaqII GACCGA 2 cut(s) 1045, 2982
TatI WGTACW 7 cut(s) 1063, 1075, 2321, 2566, 4891, 5053, 5405
TauI GCSGC 5 cut(s) 65, 95, 153, 364, 2580
TseFI GTSAC 3 cut(s) 394, 2889, 5168
Tsp45I GTSAC 3 cut(s) 394, 2889, 5168
TspGWI ACGGA 2 cut(s) 36, 4727
Vha464I CTTAAG 2 cut(s) 3442, 4961
VspI ATTAAT 1 cut(s) 3924
XbaI TCTAGA 2 cut(s) 2251, 4945
XceI RCATGY 6 cut(s) 770, 1658, 2539, 4702, 4808, 5613
XcmI CCANNNNNNNNNTGG 1 cut(s) 55
XhoI CTCGAG 1 cut(s) 4772
XmnI GAANNNNTTC 5 cut(s) 1293, 3882, 4980, 5035, 5502
ZrmI AGTACT 1 cut(s) 2568
Zsp2I ATGCAT 2 cut(s) 657, 2755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.