MD03G1058100.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0011193
Physical Location & Seq
Reverse (-)
4672823 .. 4682962
10140 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1058100.v1.1.491

Sequence Viewer

Length: 5712 bp
ATGGGGAAACAGAAAGGAGATGCAGCTAGGAGCAAGGCACGCCCTTCAAGCAGCAGTCTGGCGGCGTCGCTGGTGCCGTCGGGTTCTACTGCCACGGTGGGCTTCGGTGGCTACGTCGGCGGTTCTCGCCTCGAGGCTTCCCTCCCCAGCGAAGATTCTAAGCCCTATGCGGATGTAGATAGTGAATTGGCTCTACATTTGAAGAGGCTTGCAAGGAAAGATCCGACCACCAAGCTTAAAGCTTTGGCATCTCTATCCGCACTGCTCAAGGAAAAGTCCCCGATGGACATAACACCAATCATTCCGCAATGGGCATTTGAATACAAAAAGCTTGTGGTGGACTACAATAGGGATGTTCGGCGAGCAACTCATGACACGATGAACAATCTTGTGACTGCTGTTGGGAGAGATTTAGCTCCACATCTAAAGTCTCTGATGGGACCATGGTGGTTTTCTCAATTCGACCGACTTTCTGAAGTTGCTCAAGCTGCAAAGCGCTCATTACAGGCAGCTTTTCCAGCTCAGGAAAAGAGACTCGATGCTTTGATTTTATGCACAGCAGAGGTTTTTACCTATTTGGAAGAAAATCTGAGGCTTACGCCGCAAAGTATGTCTGAAAAAGCAACTGCTATTGATGAATTAGAAGAGATGCATCAGCAGGTGATATCTTCTTCAATTCTGGCATTGGCTACACTTCTTGATGTCTTGGTTTGCAAACAAGAAGGAAGAACAGACTCTGAGAGGATATATGCTCCACCAAAACATGCTTTGAAAGCTAGGGAAACAGCTATTTCTTTTGCTGAAAAGTTGTTCACTGCTCATAAATATTTTATAGACTTCTTGAAATCTCCGATTTCTGCTATCCGTTCAGCCACATATTCTGTATTAAGTAGTTTCATTAGAAACATTCCTCATGCCTTTAGTGAAGGAAACATGAAAACTCTTGCTGCTGCAGTATTTGGTGCATTTCAGGAGACGGATCCTGCTTGTCATTCATCAATGTGGGATGCAGTTTTGCTATTTTCTAAAAGGTTTCCTGACAGTTGGACTTCTATTAATGTACAGAAAGTAGTACTAAATCGATTTTGGAATTTTCTTAGAAATGGGTGCTTTGGATCTCAGCAGATTTCTTATCCAGCTTTACTACCTTTTTTAGACACTGTGCCGTCTAAAGCAGTAGTGGGAGAAACTTTTTTGCTTGAATTTTTCCAGAATCTATGGGCAGGAAGGAATCCATCTCATTCCTTGAATGCAGATAGATTAGCATTTCTTGGGGCTTTTAAAGACTGCTTTCTTTGGGGATTGCGTAATGCATCAAGATATTGTGACAAAGTGGATTCCATCTCTCATTTTCAAGTAAATCTTGTTAAAAATGTTCTGGTAAAGCTATTGTGGCAAGAGTACCATTTTGCTAGTAGCTCAAAACATAAGGAGAAAACCTTATCCAGATTATCAGCAGATTCATGTGAAAGTGGTTTGATTTCCAATGAGAAGACCGTGGTAACATTGAATATTATGTACCCAATGAGCTACTTGAGAGAGTTGGGGAACTGCATTGTTGGGATTCTTTCAGGCATTTATTCGCTTGAGCATGATTTGTTCTCAGCTTTCTCTGCAGAATTTGAGGAGAATTGTCTGGGTTTGTTTAATGATGATGGCAAATTGGGAACAGACAGTGAGCATGCAGAACGAATTATTCAACTTATATCATTACTTGGAGAACATGCAATGCAGAAAGGTCAAAGTTGGCCTTTGGGTTGTTTAGTTGGACCAATGTTATCTAAGTCATTCCCATTGATGAGATCACATGATTCACCTAATTGTGTGAAGATTCTATCAGTTGCTGTTTCTGTATTTGGACCACGCAAGATAGTCCAAGAGCTTCTTATCCAAAAGAATCTTTCTTGGGATCATTCTATTGATATGGGGGATAAAGAAACGGAGGCAGATCTATTCATGCAAATATTCAAGGAGAAATTTGTGCCGTGGTGTTTGCATGGGAATAGCTGCTCCTTAAGTGCTCGCCTTGATTTGTTATTTTCGTTGCTTGATGATGAATATTTCTCTGAGCAATGGGATATTGTTATCAGATATGTGACTGCTCTGGAACATTCTGGATGTGCAACTTCTTTAGACTCTGACCACATAACCATATTGTCAATGCTCTTAGAGAAAGCAAGAGATCGAATTGCAAGCACGAAAGAAGGAGAAGTATCCATGGGCAACCCAGAAAACTGGCACCATGAGCTTCTAGAGTCAGCTGCTGTTTCTTTTGCTCATTCCCCACCTTCCGGAACTTGTAATTCTCAGTTTTTGTGCACTGTTGTCGGTGGTTCAACAAAAAGCAATCAAACATCTTTTGTGTCAAGAAATACATTGATCCTGATATTTGAGGAGGTATTCAAGAAGTTGCTTTCTTTTATTCTGGCGTCCTCCTTTACATGGGTTAGGAATGCAGGTCCTCTATTGAGTCCCAATCTATTGACCGCTGTGGAAAACTATAGTGGGCTAGAATGTGAAAGTTCCGTCACCATGTTTGAGATGGCTCAGTTTGCTCTTGGAGTACTTGATGGCGCCTTGTTTTCCTTGAAGAAACTTGGTGAAGAAAGTGGGCTGGTTCCAGGTATCTTATCCGCAATTTTTATAATTGATTGGGAGTTTCTGGTATTATTAACAACAATAGATGATGCCCCTCATGATGAATCAAAGGAAAAATTGAAGGTCAGGCTGGGATTTGGTGAATCTTTTCATGCTTTTCGATGTAAGATAAGTAATCAGTTCTGGAAAGGCCTTAGCTTACACAATCGGCAGGCATTGGGGAATATTTTGATTCAGAGTATGAGATCTGCCATATTTATTGAAGATAAACTAGACACAGAAAAATTCACATCTTTGTGTTGCTTGTGGATGCTTGAAGTTCTTGATTGTGTCTCTCAGGATCAGTATGAGGAACAAAATCTGTTAGACCAACTTCTTTGCAAGGGTGATAGCTGGCCTTTGTGGATTGTTCCCGATTTTAGTAGCCCAGAGGGATTAGTGCTAAAAAATTCATCTGCTGATATCCAGGACTTTGGCCATCGCAAGTTTGTTTCATTTATTTACAAGATCATCTCAGAAGTTGGGATTGATAGAGTTGTTGCTGGTCATGTAAAACATAGTCTCCCTCCCTGCCAGGGAACAACAAATGAAGGGCTTACTCGATCTTGGCTTGCTTGTGAAATATTATGCACATGGAGGTGGCCAGGAGGAAGTGCTGTTTCTTCGTTCTTACCTTCCTTGAGTGCGTATGCGAAGAGTAGAAATTACTCTTCCCAGGAGAGCTTGTTAGATTTTATTTTCAACATTTTACTTGATGGAGCACTTATTCATGGAGGATGTGATGCACAGAGTTTTGTCTATCTATGGCCTGCTTCAAATGATGAAGTGGAGGATATTGAAGAACCCTTCTTGAGAGCTCTTGTAGCTGTACTTTTCACTCTGTTTAATGATAATATATGGGAAAGAGAGAAAGCTGTGATGCTGTTTGAACTGCTTGTGAATAAGCTTTGTGTTGGTGAAGCAATAAATGCAAACTGTTTGAGGATTCTTCCTCTGATTGTTAATGTTCTTATTCGGCCATTAAGTCAAAGAAGCATCAAACCTAATGATGAAGAGACAAAGCCTGATTCTTCTGGGGAAAATCGTGTACAGGATATTATTGAAGGCTGGCTTCAGAAAGCTATATCATTTCCACCTTTGATCACATGGCAAACAGGACAAGATATGGAAGATTGGCTTCAGTTGGTTATAGCTTGTTATCCCCTTAGTACACTGGGGGACATACAAACACCAAAGCTGGAGAGAAATGTCAGCTCTGCAGAGAGAACACTCCTACTTGAATTATTTCGGAAGCAGAGAGGTCCTGGCACATCAACTGTAATTAATCAGCTTCCAGTTGTGCAGACTTTACTATCTCGGCTAATGGTTATTTCAGTTGGTTATTGTTGGAAGGAGTTTAATGAAGAAGACTGGGAGTTTGTGTTATCTCAGTTAAGGCGCTGGATTCAGTCAGCTGTTGTAATGATGGAGGAAATTGCTGAAAATATTAATGACATTATCACAAGCCGCCTTCCTTCTGATAATCTCGATTCTGTCCTTAATAAGCTTGGGAAAATTGTCTATATTTCAGATTCCTTTACTATAGACATTGCGAAGAATGCCCTTTTATCATTTTCTCTCTGTTGTGGACCTTTTGGTCTCCAGCAAGCAGAAGTTGCAGATACCATAAACCCCTTGAGAACAGAAAGATGGGACCCCATCAAAGATCGAATTTTAGAAGGTATACTTAGATTGTTCTTTTGCACCGGCATTGCAGAGGCCATCGCACGCTCTTGTTGTGATGAGGCTGCATCTCTCATTTCATCATCCCGATCTGAACATTCGCATTTTTGGGAGTTGGTGGCCTCAAGTGTTGTCAACTCATCGACAAACGCTAGAGACAGAGCAGTGAAGTCAATAGAATTTTGGGGGCTCAGCAAAGGAGCCATTAGCGCTTTGTATGCAATCCTTTTTTCTTCAAAACCCGTTCCTTTGTTGCAGTTTGCCGCTTATTCTATAATTTCATCAGAACCTGTATTACAGTTCGCTATTGTTGAAGACAAAACTTCTTTAGACAGTGTCACTAATGGCGAAGAGGATTCATCCCCTCTTGATATATCAACAGAAACAAGCATCCATTTAAAAGAGGAAATATCTTGCATGATTGAAAAGCTACCGTATAAGGTTCTTGAAATGGATCTGGTGGCAGAGCAGAGGGTACATGTCTTCCTAGCGTGGTCTTTGTTGCTATCGCATTTATGGTCATTACCATCCTCATCACCTGCAAGGGAGAGGCTCGTCCAGTATATACAAGATTCTGCTAGTTCAGTGATTCTAGATTGCCTTTTCCAGCATATTCCTCTAGAACTGTGCATGGCACCTATTCTTAAAAAGAAAGATGCGGCGATTCCTGCAGGTATAGCAGAAGCTGCAACTGCAGCAACACACGCCATCAAAACTGGTTCAGTATTGTTTTCTGTACAATCTCTGTGGCCTGTTGAACCTGTGAAAATAGCTTCGGTTTCTGGAGCAATGTTTGGTTTGATGCTCCGCATCCTTCCTGCTTATGTAAGACAGTGGTTCAGTGATTTACGTGATCGTTCTACATCCTCTGCAATTGAATCCTTCACAAGATCATGGTGCAGCCCACCTCTCATCACGAACGAATTAAGCCTGATTAAAAAGGATGAAATAGCTGATGAGAATTTTTCGATTATCGTAAGCAAGTCAGCAAATGAGGTTGTTGCTACCTACACAAAGGATGAGACTGGACTGGACCTTGTTATCCGTCTTCCTTCATCTTACCCGTTACGACCAGTCGATGTTGATTGTCGGAGGAGCCTAGGTATTAGCGAAGTGAAGCAGAGAAAATGGTTGATGTCTATGGCGTCTTTCGTTCGTAATCAGAATGGAGCTTTAGCAGAAGCTATCAAGATATGGAAGCGCAACTTTGACAAAGAATTCGAAGGCGTGGAGGAGTGCCCTATTTGTTACAGCGTCATCCATACCGCAAACCACAGCCTTCCTCGCCTTGCCTGCAAGACCTGCAAGCACAAATTCCATTCCGCTTGCCTCTTCAAGTGGTTCTCAACTTCTCACAAATCAACTTGCCCGTTGTGCCAGTCTCCCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

1904

Amino Acids

212.51

Weight (kDa)

5.66

Isoelectric Point (pI)

47.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ltn1_1st PF22958 60 - 387 2.4e-90 E3 ubiquitin-protein ligase listerin, N-terminal domain
LTN1_E3_ligase_6th PF22999 1508 - 1752 1.6e-29 LTN1 family HEAT repeat region
UBC_like PF23009 1763 - 1846 6e-28 Ubiquitin conjugating domain-like
zf-RING_2 PF13639 1852 - 1900 7.6e-07 Ring finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G58410 AT5G58410
fragaria_vesca FvH4_3g39950 FvH4_3g39950 FvH4_3g39950
malus_domestica MD03G1058100.v1.1
prunus_persica Prupe.6G045900_v2.0.a1 Prupe.6G045900_v2.0.a1 Prupe.6G045900_v2.0.a1
pyrus_communis pycom03g04640
rosa_chinensis RchiOBHm_Chr5g0071961
rosa_laevigata RLG00000036266
rosa_multiflora Rmu_sc0012806.1_g000004
rosa_roxburghii Rroxscaffold_1G00009240
rosa_rugosa Rorug05G0415700
rosa_samantha Rh5AG471700 Rh5BG491700 Rh5CG515500 Rh5DG503100
rosa_wichuraiana Rw5G043850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2645
AarI CACCTGC 2 cut(s) 649, 4867
AasI GACNNNNNNGTC 1 cut(s) 4233
AbsI CCTCGAGG 1 cut(s) 131
Acc36I ACCTGC 5 cut(s) 649, 2447, 4867, 4982, 5630
AccB1I GGYRCC 4 cut(s) 73, 2238, 2573, 4954
AccI GTMKAC 1 cut(s) 4321
AccIII TCCGGA 1 cut(s) 2291
AclWI GGATC 8 cut(s) 215, 974, 987, 1123, 1917, 2374, 2946, 4782
AcoI YGGCCR 3 cut(s) 3073, 3239, 3614
AcuI CTGAAG 3 cut(s) 495, 3695, 3761
AcyI GRCGYC 4 cut(s) 65, 2429, 2574, 5465
AfeI AGCGCT 2 cut(s) 497, 4531
AfiI CCNNNNNNNGG 3 cut(s) 2291, 2442, 3173
AflII CTTAAG 1 cut(s) 2014
AflIII ACRYGT 1 cut(s) 4798
AjnI CCWGG 6 cut(s) 2620, 3063, 3171, 3241, 3312, 3901
Alw21I GWGCWC 4 cut(s) 2023, 2321, 3361, 3457
Alw44I GTGCAC 1 cut(s) 2317
AlwI GGATC 8 cut(s) 215, 974, 987, 1123, 1917, 2374, 2946, 4782
AlwNI CAGNNNCTG 5 cut(s) 737, 1844, 2264, 4610, 5006
Ama87I CYCGRG 1 cut(s) 131
Aor13HI TCCGGA 1 cut(s) 2291
Aor51HI AGCGCT 2 cut(s) 497, 4531
ApaLI GTGCAC 1 cut(s) 2317
ArsI GACNNNNNNTTYG 4 cut(s) 3998, 4030, 5522, 5554
AseI ATTAAT 3 cut(s) 1056, 3921, 4086
Asp700I GAANNNNTTC 2 cut(s) 2745, 3882
AspA2I CCTAGG 1 cut(s) 5419
AspLEI GCGC 5 cut(s) 498, 2576, 4038, 4532, 5523
AspS9I GGNCC 8 cut(s) 440, 1769, 1859, 2459, 3899, 4226, 4291, 5353
AsuHPI GGTGA 8 cut(s) 673, 1806, 2521, 2612, 2750, 2996, 3566, 4848
AsuII TTCGAA 1 cut(s) 5541
AvaI CYCGRG 1 cut(s) 131
AvaII GGWCC 8 cut(s) 440, 1769, 1859, 2459, 3899, 4226, 4291, 5353
AvrII CCTAGG 1 cut(s) 5419
BaeGI GKGCMC 2 cut(s) 2321, 5561
BalI TGGCCA 2 cut(s) 3075, 3241
BamHI GGATCC 1 cut(s) 979
BanI GGYRCC 4 cut(s) 73, 2238, 2573, 4954
BanII GRGCYC 2 cut(s) 3457, 4512
BbsI GAAGAC 5 cut(s) 1499, 4011, 4641, 4795, 5360
Bbv12I GWGCWC 4 cut(s) 2023, 2321, 3361, 3457
BceAI ACGGC 3 cut(s) 61, 1150, 1969
BcgI CGANNNNNNTGC 2 cut(s) 2308, 2342
BciT130I CCWGG 6 cut(s) 2622, 3065, 3173, 3243, 3314, 3903
BciVI GTATCC 1 cut(s) 2224
BclI TGATCA 1 cut(s) 3738
BfmI CTRYAG 7 cut(s) 951, 1614, 2500, 3855, 4179, 4989, 5013
BfoI RGCGCY 4 cut(s) 499, 2577, 4039, 4533
BfrI CTTAAG 1 cut(s) 2014
BfuAI ACCTGC 5 cut(s) 649, 2447, 4867, 4982, 5630
BfuI GTATCC 1 cut(s) 2224
BglII AGATCT 2 cut(s) 1948, 2843
BlnI CCTAGG 1 cut(s) 5419
BlpI GCTNAGC 1 cut(s) 4511
BmcAI AGTACT 2 cut(s) 1074, 2565
Bme1390I CCNGG 6 cut(s) 2622, 3065, 3173, 3243, 3314, 3903
Bme18I GGWCC 8 cut(s) 440, 1769, 1859, 2459, 3899, 4226, 4291, 5353
BmeT110I CYCGRG 1 cut(s) 131
BmgT120I GGNCC 8 cut(s) 440, 1769, 1859, 2459, 3899, 4226, 4291, 5353
BmrFI CCNGG 6 cut(s) 2622, 3065, 3173, 3243, 3314, 3903
BmrI ACTGGG 2 cut(s) 3821, 4018
BmuI ACTGGG 2 cut(s) 3821, 4018
BpiI GAAGAC 5 cut(s) 1499, 4011, 4641, 4795, 5360
BpmI CTGGAG 3 cut(s) 3857, 4223, 5124
Bpu10I CCTNAGC 2 cut(s) 522, 2792
Bpu1102I GCTNAGC 1 cut(s) 4511
Bpu14I TTCGAA 1 cut(s) 5541
BpuEI CTTGAG 8 cut(s) 251, 468, 1555, 1607, 3298, 3469, 4294, 4429
Bsa29I ATCGAT 1 cut(s) 1081
BsaAI YACGTR 1 cut(s) 5171
BsaHI GRCGYC 4 cut(s) 65, 2429, 2574, 5465
BsaI GGTCTC 1 cut(s) 4241
BsaJI CCNNGG 8 cut(s) 93, 443, 1497, 1985, 2217, 3172, 3312, 5419
BsaWI WCCGGW 1 cut(s) 2291
BsaXI ACNNNNNCTCC 8 cut(s) 3144, 3174, 3308, 3338, 4004, 4034, 5407, 5437
Bsc4I CCNNNNNNNGG 3 cut(s) 2291, 2442, 3173
Bse118I RCCGGY 1 cut(s) 4343
Bse3DI GCAATG 6 cut(s) 314, 1734, 2078, 4185, 4347, 5115
BseAI TCCGGA 1 cut(s) 2291
BseBI CCWGG 6 cut(s) 2622, 3065, 3173, 3243, 3314, 3903
BseCI ATCGAT 1 cut(s) 1081
BseDI CCNNGG 8 cut(s) 93, 443, 1497, 1985, 2217, 3172, 3312, 5419
BseLI CCNNNNNNNGG 3 cut(s) 2291, 2442, 3173
BseMI GCAATG 6 cut(s) 314, 1734, 2078, 4185, 4347, 5115
BseRI GAGGAG 4 cut(s) 1640, 2408, 5428, 5567
BseSI GKGCMC 2 cut(s) 2321, 5561
BseYI CCCAGC 2 cut(s) 146, 2728
BsgI GTGCAG 2 cut(s) 3959, 5239
Bsh1285I CGRYCG 1 cut(s) 466
BshNI GGYRCC 4 cut(s) 73, 2238, 2573, 4954
BshVI ATCGAT 1 cut(s) 1081
BsiEI CGRYCG 1 cut(s) 466
BsiHKAI GWGCWC 4 cut(s) 2023, 2321, 3361, 3457
BsiHKCI CYCGRG 1 cut(s) 131
BsiSI CCGG 2 cut(s) 2292, 4344
BslFI GGGAC 5 cut(s) 262, 453, 2457, 3830, 4304
BslI CCNNNNNNNGG 3 cut(s) 2291, 2442, 3173
BsmBI CGTCTC 1 cut(s) 968
BsmFI GGGAC 5 cut(s) 262, 453, 2457, 3830, 4304
BsmI GAATGC 3 cut(s) 1255, 2458, 4201
Bso31I GGTCTC 1 cut(s) 4241
BsoBI CYCGRG 1 cut(s) 131
Bsp119I TTCGAA 1 cut(s) 5541
Bsp1286I GDGCHC 6 cut(s) 2023, 2321, 3361, 3457, 4512, 5561
Bsp13I TCCGGA 1 cut(s) 2291
Bsp1407I TGTACA 3 cut(s) 1060, 3685, 5056
Bsp1720I GCTNAGC 1 cut(s) 4511
Bsp19I CCATGG 2 cut(s) 443, 2217
BspDI ATCGAT 1 cut(s) 1081
BspEI TCCGGA 1 cut(s) 2291
BspHI TCATGA 2 cut(s) 370, 2695
BspMAI CTGCAG 5 cut(s) 955, 1618, 3859, 4993, 5017
BspMI ACCTGC 5 cut(s) 649, 2447, 4867, 4982, 5630
BspPI GGATC 8 cut(s) 215, 974, 987, 1123, 1917, 2374, 2946, 4782
BspT104I TTCGAA 1 cut(s) 5541
BspT107I GGYRCC 4 cut(s) 73, 2238, 2573, 4954
BspTI CTTAAG 1 cut(s) 2014
BspTNI GGTCTC 1 cut(s) 4241
BsrDI GCAATG 6 cut(s) 314, 1734, 2078, 4185, 4347, 5115
BsrFI RCCGGY 1 cut(s) 4343
BsrGI TGTACA 3 cut(s) 1060, 3685, 5056
BssAI RCCGGY 1 cut(s) 4343
BssECI CCNNGG 8 cut(s) 93, 443, 1497, 1985, 2217, 3172, 3312, 5419
BssNAI GTATAC 1 cut(s) 4322
BssNI GRCGYC 4 cut(s) 65, 2429, 2574, 5465
BssT1I CCWWGG 3 cut(s) 443, 2217, 5419
Bst1107I GTATAC 1 cut(s) 4322
Bst2UI CCWGG 6 cut(s) 2622, 3065, 3173, 3243, 3314, 3903
Bst6I CTCTTC 7 cut(s) 197, 639, 3287, 3313, 3645, 4665, 5657
BstACI GRCGYC 4 cut(s) 65, 2429, 2574, 5465
BstAFI CTTAAG 1 cut(s) 2014
BstAPI GCANNNNNTGC 4 cut(s) 3566, 4253, 5006, 5622
BstAUI TGTACA 3 cut(s) 1060, 3685, 5056
BstBAI YACGTR 1 cut(s) 5171
BstBI TTCGAA 1 cut(s) 5541
BstDSI CCRYGG 5 cut(s) 93, 443, 1497, 1985, 2217
BstH2I RGCGCY 4 cut(s) 499, 2577, 4039, 4533
BstHHI GCGC 5 cut(s) 498, 2576, 4038, 4532, 5523
BstMCI CGRYCG 1 cut(s) 466
BstNI CCWGG 6 cut(s) 2622, 3065, 3173, 3243, 3314, 3903
BstNSI RCATGY 4 cut(s) 767, 1685, 1727, 4802
BstSCI CCNGG 6 cut(s) 2620, 3063, 3171, 3241, 3312, 3901
BstSFI CTRYAG 7 cut(s) 951, 1614, 2500, 3855, 4179, 4989, 5013
BstSLI GKGCMC 2 cut(s) 2321, 5561
BstV2I GAAGAC 5 cut(s) 1499, 4011, 4641, 4795, 5360
BstX2I RGATCY 6 cut(s) 220, 979, 1115, 1948, 2843, 4774
BstXI CCANNNNNNTGG 2 cut(s) 2235, 3071
BstYI RGATCY 6 cut(s) 220, 979, 1115, 1948, 2843, 4774
BstZ17I GTATAC 1 cut(s) 4322
Bsu15I ATCGAT 1 cut(s) 1081
BsuI GTATCC 1 cut(s) 2224
BsuTUI ATCGAT 1 cut(s) 1081
BtgI CCRYGG 5 cut(s) 93, 443, 1497, 1985, 2217
BtgZI GCGATG 2 cut(s) 3062, 4345
BtsI GCAGTG 3 cut(s) 260, 813, 4491
BveI ACCTGC 5 cut(s) 649, 2447, 4867, 4982, 5630
CaiI CAGNNNCTG 5 cut(s) 737, 1844, 2264, 4610, 5006
CciI TCATGA 2 cut(s) 370, 2695
CfoI GCGC 5 cut(s) 498, 2576, 4038, 4532, 5523
Cfr10I RCCGGY 1 cut(s) 4343
Cfr13I GGNCC 8 cut(s) 440, 1769, 1859, 2459, 3899, 4226, 4291, 5353
ClaI ATCGAT 1 cut(s) 1081
CseI GACGC 4 cut(s) 54, 2418, 5454, 5563
CspCI CAANNNNNGTGG 2 cut(s) 5048, 5083
DinI GGCGCC 1 cut(s) 2575
DraI TTTAAA 2 cut(s) 1282, 4719
DrdI GACNNNNNNGTC 1 cut(s) 4233
DseDI GACNNNNNNGTC 1 cut(s) 4233
EaeI YGGCCR 3 cut(s) 3073, 3239, 3614
Eam1104I CTCTTC 7 cut(s) 197, 639, 3287, 3313, 3645, 4665, 5657
EarI CTCTTC 7 cut(s) 197, 639, 3287, 3313, 3645, 4665, 5657
Ecl136II GAGCTC 1 cut(s) 3455
Eco130I CCWWGG 3 cut(s) 443, 2217, 5419
Eco147I AGGCCT 1 cut(s) 2790
Eco24I GRGCYC 2 cut(s) 3457, 4512
Eco31I GGTCTC 1 cut(s) 4241
Eco32I GATATC 2 cut(s) 666, 3061
Eco47I GGWCC 8 cut(s) 440, 1769, 1859, 2459, 3899, 4226, 4291, 5353
Eco47III AGCGCT 2 cut(s) 497, 4531
Eco53kI GAGCTC 1 cut(s) 3455
Eco57I CTGAAG 3 cut(s) 495, 3695, 3761
Eco88I CYCGRG 1 cut(s) 131
EcoICRI GAGCTC 1 cut(s) 3455
EcoO109I RGGNCCY 3 cut(s) 2459, 3899, 4291
EcoRI GAATTC 1 cut(s) 5537
EcoRII CCWGG 6 cut(s) 2620, 3063, 3171, 3241, 3312, 3901
EcoRV GATATC 2 cut(s) 666, 3061
EcoT14I CCWWGG 3 cut(s) 443, 2217, 5419
EcoT22I ATGCAT 2 cut(s) 654, 1315
EcoT38I GRGCYC 2 cut(s) 3457, 4512
EgeI GGCGCC 1 cut(s) 2575
EheI GGCGCC 1 cut(s) 2575
ErhI CCWWGG 3 cut(s) 443, 2217, 5419
Esp3I CGTCTC 1 cut(s) 968
FaqI GGGAC 5 cut(s) 262, 453, 2457, 3830, 4304
FbaI TGATCA 1 cut(s) 3738
FblI GTMKAC 1 cut(s) 4321
FriOI GRGCYC 2 cut(s) 3457, 4512
GlaI GCGC 5 cut(s) 497, 2575, 4037, 4531, 5522
GsaI CCCAGC 2 cut(s) 150, 2732
GsuI CTGGAG 3 cut(s) 3857, 4223, 5124
HaeII RGCGCY 4 cut(s) 499, 2577, 4039, 4533
HapII CCGG 2 cut(s) 2292, 4344
HgaI GACGC 4 cut(s) 54, 2418, 5454, 5563
HhaI GCGC 5 cut(s) 498, 2576, 4038, 4532, 5523
Hin1I GRCGYC 4 cut(s) 65, 2429, 2574, 5465
Hin6I GCGC 5 cut(s) 496, 2574, 4036, 4530, 5521
HinP1I GCGC 5 cut(s) 496, 2574, 4036, 4530, 5521
HincII GTYRAC 1 cut(s) 4456
HindII GTYRAC 1 cut(s) 4456
HindIII AAGCTT 5 cut(s) 233, 240, 329, 3542, 4142
HpaII CCGG 2 cut(s) 2292, 4344
HphI GGTGA 8 cut(s) 673, 1806, 2521, 2612, 2750, 2996, 3566, 4848
Hpy166II GTNNAC 8 cut(s) 340, 813, 2319, 3686, 3809, 4226, 4322, 4456
Hpy8I GTNNAC 8 cut(s) 340, 813, 2319, 3686, 3809, 4226, 4322, 4456
Hpy99I CGWCG 3 cut(s) 70, 82, 119
HpyCH4IV ACGT 2 cut(s) 114, 5170
HpySE526I ACGT 2 cut(s) 114, 5170
Hsp92I GRCGYC 4 cut(s) 65, 2429, 2574, 5465
HspAI GCGC 5 cut(s) 496, 2574, 4036, 4530, 5521
KasI GGCGCC 1 cut(s) 2573
KflI GGGWCCC 1 cut(s) 4291
Kpn2I TCCGGA 1 cut(s) 2291
Ksp22I TGATCA 1 cut(s) 3738
MaeII ACGT 2 cut(s) 114, 5170
MaeIII GTNAC 8 cut(s) 391, 1325, 1501, 2095, 2527, 4657, 5385, 5567
MfeI CAATTG 1 cut(s) 5193
MflI RGATCY 6 cut(s) 220, 979, 1115, 1948, 2843, 4774
MhlI GDGCHC 6 cut(s) 2023, 2321, 3361, 3457, 4512, 5561
MlsI TGGCCA 2 cut(s) 3075, 3241
MluNI TGGCCA 2 cut(s) 3075, 3241
Mly113I GGCGCC 1 cut(s) 2574
MlyI GAGTC 5 cut(s) 528, 728, 2129, 2264, 2479
MmeI TCCRAC 5 cut(s) 248, 1025, 1747, 3965, 5390
Mox20I TGGCCA 2 cut(s) 3075, 3241
Mph1103I ATGCAT 2 cut(s) 654, 1315
MroI TCCGGA 1 cut(s) 2291
MroXI GAANNNNTTC 2 cut(s) 2745, 3882
MscI TGGCCA 2 cut(s) 3075, 3241
MslI CAYNNNNRTG 3 cut(s) 1000, 2893, 3235
Msp20I TGGCCA 2 cut(s) 3075, 3241
MspA1I CMGCKG 3 cut(s) 2261, 2489, 4052
MspCI CTTAAG 1 cut(s) 2014
MspI CCGG 2 cut(s) 2292, 4344
MspR9I CCNGG 6 cut(s) 2622, 3065, 3173, 3243, 3314, 3903
MunI CAATTG 1 cut(s) 5193
Mva1269I GAATGC 3 cut(s) 1255, 2458, 4201
MvaI CCWGG 6 cut(s) 2622, 3065, 3173, 3243, 3314, 3903
NarI GGCGCC 1 cut(s) 2574
NcoI CCATGG 2 cut(s) 443, 2217
NmeAIII GCCGAG 1 cut(s) 3934
NmuCI GTSAC 5 cut(s) 391, 1325, 2095, 2527, 4657
NsiI ATGCAT 2 cut(s) 654, 1315
NspI RCATGY 4 cut(s) 767, 1685, 1727, 4802
NspV TTCGAA 1 cut(s) 5541
PaeI GCATGC 1 cut(s) 1685
PaeR7I CTCGAG 1 cut(s) 131
PagI TCATGA 2 cut(s) 370, 2695
PaqCI CACCTGC 2 cut(s) 649, 4867
PceI AGGCCT 1 cut(s) 2790
PciI ACATGT 1 cut(s) 4798
PcsI WCGNNNNNNNCGW 2 cut(s) 74, 111
PctI GAATGC 3 cut(s) 1255, 2458, 4201
PdmI GAANNNNTTC 2 cut(s) 2745, 3882
PflFI GACNNNGTC 1 cut(s) 4655
PfoI TCCNGGA 1 cut(s) 3063
PleI GAGTC 5 cut(s) 528, 728, 2129, 2263, 2478
PluTI GGCGCC 1 cut(s) 2577
PpsI GAGTC 5 cut(s) 528, 728, 2129, 2263, 2478
Ppu21I YACGTR 1 cut(s) 5171
PpuMI RGGWCCY 3 cut(s) 2459, 3899, 4291
PscI ACATGT 1 cut(s) 4798
PshBI ATTAAT 3 cut(s) 1056, 3921, 4086
PsiI TTATAA 1 cut(s) 2645
Psp124BI GAGCTC 1 cut(s) 3457
Psp5II RGGWCCY 3 cut(s) 2459, 3899, 4291
Psp6I CCWGG 6 cut(s) 2620, 3063, 3171, 3241, 3312, 3901
PspFI CCCAGC 2 cut(s) 146, 2728
PspGI CCWGG 6 cut(s) 2620, 3063, 3171, 3241, 3312, 3901
PspPI GGNCC 8 cut(s) 440, 1769, 1859, 2459, 3899, 4226, 4291, 5353
PspPPI RGGWCCY 3 cut(s) 2459, 3899, 4291
PspXI VCTCGAGB 1 cut(s) 131
PsrI GAACNNNNNNTAC 2 cut(s) 4599, 4631
PstI CTGCAG 5 cut(s) 955, 1618, 3859, 4993, 5017
PstNI CAGNNNCTG 5 cut(s) 737, 1844, 2264, 4610, 5006
PsuI RGATCY 6 cut(s) 220, 979, 1115, 1948, 2843, 4774
PsyI GACNNNGTC 1 cut(s) 4655
PvuII CAGCTG 2 cut(s) 2261, 4052
RseI CAYNNNNRTG 3 cut(s) 1000, 2893, 3235
SacI GAGCTC 1 cut(s) 3457
Sau96I GGNCC 8 cut(s) 440, 1769, 1859, 2459, 3899, 4226, 4291, 5353
SbfI CCTGCAGG 1 cut(s) 4993
ScaI AGTACT 2 cut(s) 1074, 2565
SchI GAGTC 5 cut(s) 528, 728, 2129, 2264, 2479
ScrFI CCNGG 6 cut(s) 2622, 3065, 3173, 3243, 3314, 3903
SdaI CCTGCAGG 1 cut(s) 4993
SduI GDGCHC 6 cut(s) 2023, 2321, 3361, 3457, 4512, 5561
SfcI CTRYAG 7 cut(s) 951, 1614, 2500, 3855, 4179, 4989, 5013
SfoI GGCGCC 1 cut(s) 2575
Sfr274I CTCGAG 1 cut(s) 131
SfuI TTCGAA 1 cut(s) 5541
SinI GGWCC 8 cut(s) 440, 1769, 1859, 2459, 3899, 4226, 4291, 5353
SlaI CTCGAG 1 cut(s) 131
SmiMI CAYNNNNRTG 3 cut(s) 1000, 2893, 3235
SphI GCATGC 1 cut(s) 1685
Sse8387I CCTGCAGG 1 cut(s) 4993
SseBI AGGCCT 1 cut(s) 2790
SspDI GGCGCC 1 cut(s) 2573
SspI AATATT 7 cut(s) 827, 1513, 1965, 2060, 2824, 3222, 4084
SstI GAGCTC 1 cut(s) 3457
StuI AGGCCT 1 cut(s) 2790
StyD4I CCNGG 6 cut(s) 2620, 3063, 3171, 3241, 3312, 3901
StyI CCWWGG 3 cut(s) 443, 2217, 5419
TaiI ACGT 2 cut(s) 117, 5173
TaqII GACCGA 1 cut(s) 480
TatI WGTACW 7 cut(s) 1060, 1072, 2563, 3466, 3685, 3806, 5056
TauI GCSGC 5 cut(s) 65, 604, 4107, 4586, 4982
TseFI GTSAC 5 cut(s) 391, 1325, 2095, 2527, 4657
Tsp45I GTSAC 5 cut(s) 391, 1325, 2095, 2527, 4657
TspGWI ACGGA 5 cut(s) 854, 992, 1955, 2515, 5354
Tth111I GACNNNGTC 1 cut(s) 4655
Vha464I CTTAAG 1 cut(s) 2014
VneI GTGCAC 1 cut(s) 2317
VpaK11BI GGWCC 8 cut(s) 440, 1769, 1859, 2459, 3899, 4226, 4291, 5353
VspI ATTAAT 3 cut(s) 1056, 3921, 4086
XbaI TCTAGA 3 cut(s) 2251, 4912, 4939
XceI RCATGY 4 cut(s) 767, 1685, 1727, 4802
XcmI CCANNNNNNNNNTGG 1 cut(s) 2539
XhoI CTCGAG 1 cut(s) 131
XmaJI CCTAGG 1 cut(s) 5419
XmiI GTMKAC 1 cut(s) 4321
XmnI GAANNNNTTC 2 cut(s) 2745, 3882
ZrmI AGTACT 2 cut(s) 1074, 2565
Zsp2I ATGCAT 2 cut(s) 654, 1315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.