Rh5CG515500

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0011193
Physical Location & Seq
Reverse (-)
71971122 .. 71982334
11213 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG515500.1

Sequence Viewer

Length: 4878 bp
ATGGGGAAGCAGAAGGGAGACGGAGCTAGGAGCAAGGCCCGCCCTTCAAGTAGCAGTTTGGCGGCGTCGCTGTTGCCGTCGGGTTCTACTGCGGCGGTGGGCTTCGGTGGCTACGTCGGAGGCTCTCGCCTGGATGCTCCTCCTTCCGGCGATGATTCAAGGCCCTTTTTGGAAGTAGATAGTGATTTGGCTCTACATTTGAAGAGGCTTGCAAGGAAAGATCCCACCACCAAGCTTAAAGCTTTGGCATCTCTATCCACACTGTTGAAGGAAAAGTCCACGAAGGACATAGTAGCGGCCATTCCACAATGGGGATTTGAATACAAAAGGCTTGTGGTGGACTATAATAGGGATGTGCGGCGAGCAACTCATGACACAATGACCATTCTTGTCACCACCGTTGGGAGAGATTTAGCGCCACAGCTGAAATCTTTGATGGGACCGTGGTGGTTTTCTCAATTTGACCCAGTCTCTGAAGTTTCTCAAGCTGCAAAGCGATCATTTCAGGCAGTCTTTTCAGCTCCAGAAAAGAGACTAGATGCTTTAATTTTATGCACGGCAGAGGTTTTTATTTATTTGGAAGAAAATCTGAGGCTTACACCGCAAAATATGTCCGATAAAGGAACTGCTCTGGATGAATTGCAAGAGATGCATCAGCAGGTGATATCTTCATCATTGCTGGCATTGGCCACACTTCTTGATGTCTTGGTTTGCTTACAACTAGAAAGACCAGGCACCGAAAGCATAGCTGCTCAACCAAAACATGCTTTGAAGGCTAGGGAAACTGCTATTTCTTGTGCCGACAAGTTGTTCACTGCTCATAAATATTTCTTAGACTTCTTGAAATCCCCAAGTCCTGCTATCCGTTCAGCCACATATTCTGTATTGAGTAGTTTTATAAAAAACGTCCCTCAAGCCTTTAATGAAGGAAATATGAAAACTCTTGCTGCTGCACTATTAGGTGGATTTCAGGAGAAGGATCCTGCTTGTCATTCATCAATGTGGGATGCAATTTTACTGTTTTCTAAAAAATTTCCTGAAAGTTGGACTTCTGTCAATGTACAGAAAACTGTACTGAATCGGTTTTGGGATTTCCTTAGAAATAGGTGCTTTGGATCTCAACAGGTTTCTTATCCATCTTTGGTTCTATTCTTACACACCGTGCCATCTAAAGCAGTCGTTGCAGAAACTTTTTTTCTTGAATTCTTCAAGAACCTCTGGGCAGGAAGGAATCCATCGCATTCCTTAAATGCAGATAGAGTGGCATTTTTTCGAGCATTACAAGAATGCTTTCTTTGGGCATTGCATAATGCCTCAAGATATTGCAATGGAGTGGATTCCATCAGTGATTTTCGAGCAACTCTTGTTAAAAATGTTCTGGTAAAGCTTTTGTGGCAAGACTACATTTTCTCTAGTAGCTCAAGAAAGGAGGAGAAAACATCCCCCGGATTATCAGTAGATTCATGTGAAAGTGATTTAGCTTCCAACAAGAAGACAGTTGAAACATTGAATATCACGTACCCAATGAGCTACTTTAGAGAGTTGGCAAATTGCATTGTTGGGGTTCTTTCAGGCATTGATTTGCTGGAGCATGATTTGCTCTCAGTTTTTGCTGCAGAATTTCAGGAGAATTGTCAGAGTTTCTTTCAGCATGCTAGTAACTTGGAAAGAGAAAGTGAGTTTGCGGAGCGAGTTATACAGTTTATATCATTACTTGGAGAACATGCAATGCAGAATGGTCGAGGTTGGCCTTTGGCTTCTCTAGTTGGACCAATGTTGGCTAATTCCTTTACATTGATGAGATCACATGATTCACCAAGTTGTGTGAAGATTCTAGCAGCGGCTGTTTCTGTATTTGGACCTCACAAGATAGTCCACGAACTTCTTATTCACAATACGTCTCCACATAGTTATTCTCCTGATGAGGGTGACAGAGCATTAGAGGAAGATAGATTCCTGCAAATGTTCAATGGAACTTTTGTTCCATGGTGTCTTAGTGGGAATAGTTACTCTTTAAGTGCTCGTGTTGACCTGTTACTTGCATTGCTTGATGATGAATATTTCACTGAGCAGTGGGATAGTGTTATCAGATATGCCACTAACCTGGAGCATTCTGGATCTGCACCCTGCTCTTTAGATTCTGACCGGATAACTATCTTAGCACTGCTTTTACAGAAAGTAAGAAACAGAATCACAAACACAAAGGTAGGAGTTTCCATCAGCACAAAAATGGGCAACCCAGATCATTGGCATCATGAGCTTCTAGAATCAACTGCTGTTGCTGTTGCTCGATGCTCTCCTCCTCTCGGAGCTTCTAATTCTCAATTCTTGTGTACTGTTGTTGGTGGTTCAACAAAGACCAATCAAATTTCTCTGGTGTCAAGAAATACATTGATCCTGATATTTGAAGAGGTTTTCAAGAAGTTACTTTCTTTTATTATGGCATCCTCTTTTACTTGGGTTAGGGATGCAGGTTCTCTATTGACTGCCGGGGCAAACACTATTGGGCCAGAATTTGAAAATTCTGTGAGCATGTTTGAGATGGCTCAATTTGCTCTTGAAGTACTTGATGGCGGCTTATATTCATTAAAGACACTTGGTGAAGAAAGTGGGTTGAGTTCAGTTATTTTAGCTGCAATTTTTCTGATTGACTGGGAGTTTCTGGTATTACTGACAACGATAGATGATGCACCTGATGATAAATCAAGAGAAAAATTGAAGGCCAGGCTGGGATTTGGTGAATCTTTTCATGCATTTAGATGTAAGATAAGTAATCAATTCTGGAAAACACTCAGCTCACACAATCGAAAGGCACTTGGAAAAATTTTGATTCAGTGTATGAGATCCGCCATCTTCAATGAAGAGGAATTAGACACAGAGAAGTTCACATCTCTGTGCTGCTTGTGGATGCTTGAAATTCTGGACTGTCTCTCTCAGGATCCCTATGAGGAACAAAATCTGCTGGACCAACTTCTCTGCCAAGGTGATAGATGGCCTTTGTGGATAGTTCCGGATTTTAGCAGGCCAGAGGGAATAGTTGCCAAGGACTTCTCTATCCAAGACTTTGGCCACCGCAAGTTTATTTCATTTATTGACAAGATGATATCAGAAATTGGGATTGATAGAGTTGTTGGGGGTTATGCTAGATATACTCTCCCTCCCTCTGTGGAAGCAACAAATGAAAAGCCGACTCGATCCTGGCTTGCTGCTGAAATATTATGCTCGTGGAAATGGCCAGGAGGAAGTGCTGTAGCTTCATTCTTACCTTCATTGAGTGCCTATGCGAAGAGTAAAAATTTCTCTTCCCAGGAGAGCTTGGTAGATTTTATTTTCAACATTCTAATTGATGGCACACTTGTTCAGGGAGGATGTGCTGCACAGAATTTTGTCTATCTGTGTCCTACTTCAAGTGATGAAGTGGAGGATATTGAAGAACCATTCTTAAGAGCTCTTGTAGCTTTTCTTTTAACTTTGTTTAATGATAAAATATGGGGTACCGAGAAAGCTATGGAGCTGTTTGCATTGCTTGTAAATAAACTTTATGTTGGAGAAGCAACAAATGCAAACTGTTTGAGGATTCTTCCTCTGATTGTTAATGTTCTTATTCAACCATTAAGTCAAAGAAGCATCAGATGTAATGACTCTAGTGGAGACGCTCAACATGATTCTTCTGGGGAAAATCATGTGCAAGATGTTATTGAAGGCTGGCTAAAAAAAGCTCTATCATTTCCACCTTTGATCACGTGGTGGCAAACAGGAGAAGATATGGAAGATTGGATGCAGCTGGTTATTTCTTGTTATCCCATTAGTGCAGTAGAGGACATACAAACACCGAAGCTGGAGAGAAAGATCAGCTCGGTAGAGAGAAAGCTCCTACTTGAATTATTCCGGAAGCAGAGACATGGTGTTGGCGCACCAGCTGTTATTAATCAGCTTCCAGTTGTGCAGATGTTGCTATCAAAGCTTATGGTCGTTTCAGTTGGTTATTGTTGGAAAGAATTTGATGAAGAAGATTGGGAGTTTGTGTTATCTCAGATAAGACGCTGGCTGCAGTCTATTGTTGTAATGATGGAGGAAATTGCTGAAAATGTCAACGACACTATCACTAGCAGCTTTACTTCTGATAACTTGGATGATGTCGTTGATAACCTTGGCAAAATTGTTTTCGTTTCAGATCCCTTTCCTATAGACATTGCCAAAAATGCCCTTTTATCATTTTCTCTGTCCTGTGGACCTTTTGGTCGCCAACAAGCAGAAGATGCAGATAACTTAAATCCCATGAGAACAGAAAGATGGGACCCCATCAAAAATAGAATTTTAGAAGGCATCCTTCGATTGTTGTTCTGCACGGGTATTGCTGAGGCCATTGCAAGCTCCTGTTGTCACGAGGCTGCATTTATTGTATCAGCGGCGCGATTTGAACATTCCTATTTCTGGGAATTGGTGGCCTCAAATGTGGTCAACTCATCAACAGATGCCATAGATAGAGCAGTGAAGTCAGTTGAATTCTGGGGGCTGAGCAAAGGACCCATTAGCTCTTTGTATGCAATCCTTTTTTCTGCCAAAACAGTTCCTCTGTTGCAGTTCGCTGCTTACTTCATTCTTTCAACTGAACTTGTTTTACGCCTGGCTATTGTTGAAGAAGACAAAAGCTACTTGGATGGCGTTAGTAACAATGAAGAGGTTTCATCCTCTCTTGACATGTCGACAGAAACAGATATTCATTTGAGAGCTGAAATATCTTGCATGATTGAAAAGTTGCCTAGTAATGTTCTCGAGATGGATTTAGTGGCAGATCAAAGGGTAAATATGCTTTCAATAATAGTCAAAAATACTCCTTGCTTGCATTGTATTTGTCTCTTGCTTGGTATTTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

1625

Amino Acids

181.16

Weight (kDa)

5.29

Isoelectric Point (pI)

43.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ltn1_1st PF22958 60 - 386 1.5e-90 E3 ubiquitin-protein ligase listerin, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G58410 AT5G58410
fragaria_vesca FvH4_3g39950 FvH4_3g39950 FvH4_3g39950
malus_domestica MD03G1058100.v1.1
prunus_persica Prupe.6G045900_v2.0.a1 Prupe.6G045900_v2.0.a1 Prupe.6G045900_v2.0.a1
pyrus_communis pycom03g04640
rosa_chinensis RchiOBHm_Chr5g0071961
rosa_laevigata RLG00000036266
rosa_multiflora Rmu_sc0012806.1_g000004
rosa_roxburghii Rroxscaffold_1G00009240
rosa_rugosa Rorug05G0415700
rosa_samantha Rh5AG471700 Rh5BG491700 Rh5CG515500 Rh5DG503100
rosa_wichuraiana Rw5G043850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 899
AarI CACCTGC 1 cut(s) 649
AasI GACNNNNNNGTC 1 cut(s) 4239
Acc36I ACCTGC 2 cut(s) 649, 2462
Acc65I GGTACC 1 cut(s) 3494
AccB1I GGYRCC 2 cut(s) 734, 3494
AccI GTMKAC 1 cut(s) 4706
AccII CGCG 1 cut(s) 4414
AccIII TCCGGA 2 cut(s) 3010, 3888
AcoI YGGCCR 4 cut(s) 297, 687, 3067, 3233
AcuI CTGAAG 1 cut(s) 495
AcvI CACGTG 1 cut(s) 3744
AcyI GRCGYC 1 cut(s) 65
AdeI CACNNNGTG 3 cut(s) 1162, 2600, 3747
AfaI GTAC 6 cut(s) 1062, 1074, 1520, 2335, 2565, 3496
AfiI CCNNNNNNNGG 6 cut(s) 146, 311, 402, 1925, 2306, 4434
AflII CTTAAG 1 cut(s) 3442
AflIII ACRYGT 1 cut(s) 4701
AjnI CCWGG 8 cut(s) 129, 730, 2103, 2723, 3197, 3235, 3306, 4626
AloI GAACNNNNNNTCC 4 cut(s) 1965, 1966, 1997, 1998
Alw21I GWGCWC 2 cut(s) 2023, 3451
Alw26I GTCTC 8 cut(s) 12, 475, 526, 1905, 2933, 3645, 3892, 4862
AlwNI CAGNNNCTG 2 cut(s) 473, 1844
Ama87I CYCGRG 1 cut(s) 4775
Aor13HI TCCGGA 2 cut(s) 3010, 3888
AseI ATTAAT 1 cut(s) 3927
Asp700I GAANNNNTTC 2 cut(s) 1290, 2745
Asp718I GGTACC 1 cut(s) 3494
AspLEI GCGC 3 cut(s) 418, 3914, 4414
AsuC2I CCSGG 2 cut(s) 1446, 2491
AsuHPI GGTGA 7 cut(s) 385, 673, 1806, 1940, 2612, 2750, 2996
AvaI CYCGRG 1 cut(s) 4775
AvaII GGWCC 7 cut(s) 440, 1769, 1859, 2965, 4232, 4297, 4526
BalI TGGCCA 3 cut(s) 689, 3069, 3235
BamHI GGATCC 2 cut(s) 979, 2938
BanI GGYRCC 2 cut(s) 734, 3494
BanII GRGCYC 1 cut(s) 3451
BauI CACGAG 3 cut(s) 2022, 3223, 4385
BbrPI CACGTG 1 cut(s) 3744
BbsI GAAGAC 2 cut(s) 1499, 4650
Bbv12I GWGCWC 2 cut(s) 2023, 3451
BbvCI CCTCAGC 1 cut(s) 4359
BceAI ACGGC 2 cut(s) 61, 573
BcgI CGANNNNNNTGC 2 cut(s) 1721, 1755
BciT130I CCWGG 8 cut(s) 131, 732, 2105, 2725, 3199, 3237, 3308, 4628
BclI TGATCA 1 cut(s) 3738
BcnI CCSGG 2 cut(s) 1446, 2491
BcoDI GTCTC 8 cut(s) 12, 475, 526, 1905, 2933, 3645, 3892, 4862
BfmI CTRYAG 4 cut(s) 1614, 3249, 4049, 4185
BfoI RGCGCY 1 cut(s) 419
BfrI CTTAAG 1 cut(s) 3442
BfuAI ACCTGC 2 cut(s) 649, 2462
BlpI GCTNAGC 1 cut(s) 4517
BmcAI AGTACT 1 cut(s) 2565
Bme18I GGWCC 7 cut(s) 440, 1769, 1859, 2965, 4232, 4297, 4526
BmeT110I CYCGRG 1 cut(s) 4775
BmiI GGNNCC 8 cut(s) 441, 736, 981, 2940, 3496, 4298, 4299, 4528
BmrI ACTGGG 2 cut(s) 461, 2662
BmuI ACTGGG 2 cut(s) 461, 2662
BoxI GACNNNNGTC 1 cut(s) 1052
BpiI GAAGAC 2 cut(s) 1499, 4650
BpmI CTGGAG 4 cut(s) 507, 1607, 2126, 3860
Bpu10I CCTNAGC 1 cut(s) 4359
Bpu1102I GCTNAGC 1 cut(s) 4517
BpuEI CTTGAG 4 cut(s) 468, 897, 1300, 1405
BpuMI CCSGG 2 cut(s) 1446, 2491
BsaAI YACGTR 2 cut(s) 1518, 3744
BsaBI GATNNNNATC 1 cut(s) 2153
BsaHI GRCGYC 1 cut(s) 65
BsaJI CCNNGG 8 cut(s) 443, 1444, 1985, 2490, 2980, 3042, 3306, 4150
BsaWI WCCGGW 3 cut(s) 2145, 3010, 3888
BsaXI ACNNNNNCTCC 4 cut(s) 3142, 3172, 4010, 4040
Bsc4I CCNNNNNNNGG 6 cut(s) 146, 311, 402, 1925, 2306, 4434
Bse1I ACTGG 3 cut(s) 467, 2657, 3938
Bse3DI GCAATG 8 cut(s) 674, 1301, 1333, 1734, 2042, 3521, 4191, 4365
Bse8I GATNNNNATC 1 cut(s) 2153
BseAI TCCGGA 2 cut(s) 3010, 3888
BseBI CCWGG 8 cut(s) 131, 732, 2105, 2725, 3199, 3237, 3308, 4628
BseDI CCNNGG 8 cut(s) 443, 1444, 1985, 2490, 2980, 3042, 3306, 4150
BseJI GATNNNNATC 1 cut(s) 2153
BseLI CCNNNNNNNGG 6 cut(s) 146, 311, 402, 1925, 2306, 4434
BseMI GCAATG 8 cut(s) 674, 1301, 1333, 1734, 2042, 3521, 4191, 4365
BseMII CTCAG 8 cut(s) 581, 1617, 2058, 2806, 2948, 4046, 4350, 4508
BseNI ACTGG 3 cut(s) 467, 2657, 3938
BseRI GAGGAG 4 cut(s) 129, 1445, 2289, 2292
BseYI CCCAGC 1 cut(s) 2728
BsgI GTGCAG 6 cut(s) 936, 2106, 3360, 3831, 3965, 4330
Bsh1236I CGCG 1 cut(s) 4414
BshNI GGYRCC 2 cut(s) 734, 3494
BsiHKAI GWGCWC 2 cut(s) 2023, 3451
BsiHKCI CYCGRG 1 cut(s) 4775
BsiSI CCGG 6 cut(s) 147, 1446, 2146, 2490, 3011, 3889
BslFI GGGAC 3 cut(s) 453, 893, 4310
BslI CCNNNNNNNGG 6 cut(s) 146, 311, 402, 1925, 2306, 4434
BsmAI GTCTC 8 cut(s) 12, 475, 526, 1905, 2933, 3645, 3892, 4862
BsmBI CGTCTC 3 cut(s) 12, 1905, 3645
BsmFI GGGAC 3 cut(s) 453, 893, 4310
BsmI GAATGC 3 cut(s) 1240, 1292, 2110
BsoBI CYCGRG 1 cut(s) 4775
Bsp1286I GDGCHC 2 cut(s) 2023, 3451
Bsp13I TCCGGA 2 cut(s) 3010, 3888
Bsp1407I TGTACA 1 cut(s) 1060
Bsp1720I GCTNAGC 1 cut(s) 4517
Bsp19I CCATGG 1 cut(s) 1985
BspCNI CTCAG 8 cut(s) 582, 1616, 2059, 2805, 2947, 4045, 4351, 4509
BspEI TCCGGA 2 cut(s) 3010, 3888
BspFNI CGCG 1 cut(s) 4414
BspHI TCATGA 2 cut(s) 370, 2254
BspLI GGNNCC 8 cut(s) 441, 736, 981, 2940, 3496, 4298, 4299, 4528
BspMAI CTGCAG 2 cut(s) 1618, 4053
BspMI ACCTGC 2 cut(s) 649, 2462
BspT107I GGYRCC 2 cut(s) 734, 3494
BspTI CTTAAG 1 cut(s) 3442
BsrDI GCAATG 8 cut(s) 674, 1301, 1333, 1734, 2042, 3521, 4191, 4365
BsrGI TGTACA 1 cut(s) 1060
BsrI ACTGG 3 cut(s) 467, 2657, 3938
BssECI CCNNGG 8 cut(s) 443, 1444, 1985, 2490, 2980, 3042, 3306, 4150
BssNI GRCGYC 1 cut(s) 65
BssSI CACGAG 3 cut(s) 2022, 3223, 4385
BssT1I CCWWGG 4 cut(s) 1985, 2980, 3042, 4150
Bst2BI CACGAG 3 cut(s) 2022, 3223, 4385
Bst2UI CCWGG 8 cut(s) 131, 732, 2105, 2725, 3199, 3237, 3308, 4628
Bst6I CTCTTC 6 cut(s) 197, 2403, 2856, 3281, 3307, 4674
BstACI GRCGYC 1 cut(s) 65
BstAFI CTTAAG 1 cut(s) 3442
BstAPI GCANNNNNTGC 6 cut(s) 649, 1181, 1597, 3560, 3952, 4259
BstAUI TGTACA 1 cut(s) 1060
BstBAI YACGTR 2 cut(s) 1518, 3744
BstDSI CCRYGG 2 cut(s) 443, 1985
BstFNI CGCG 1 cut(s) 4414
BstH2I RGCGCY 1 cut(s) 419
BstHHI GCGC 3 cut(s) 418, 3914, 4414
BstMAI GTCTC 8 cut(s) 12, 475, 526, 1905, 2933, 3645, 3892, 4862
BstNI CCWGG 8 cut(s) 131, 732, 2105, 2725, 3199, 3237, 3308, 4628
BstNSI RCATGY 5 cut(s) 767, 1655, 1727, 2536, 4705
BstPAI GACNNNNGTC 1 cut(s) 1052
BstSFI CTRYAG 4 cut(s) 1614, 3249, 4049, 4185
BstUI CGCG 1 cut(s) 4414
BstV2I GAAGAC 2 cut(s) 1499, 4650
BstX2I RGATCY 7 cut(s) 220, 979, 1115, 2117, 2843, 2938, 4174
BstXI CCANNNNNNTGG 3 cut(s) 2104, 2247, 3065
BstYI RGATCY 7 cut(s) 220, 979, 1115, 2117, 2843, 2938, 4174
BtgI CCRYGG 2 cut(s) 443, 1985
BtgZI GCGATG 2 cut(s) 165, 1221
BtsI GCAGTG 4 cut(s) 813, 2078, 2162, 4497
BtsIMutI CAGTG 8 cut(s) 260, 813, 1351, 2064, 2078, 2162, 2840, 4497
BveI ACCTGC 2 cut(s) 649, 2462
CaiI CAGNNNCTG 2 cut(s) 473, 1844
CciI TCATGA 2 cut(s) 370, 2254
CfoI GCGC 3 cut(s) 418, 3914, 4414
CseI GACGC 3 cut(s) 54, 3662, 4050
Csp6I GTAC 6 cut(s) 1061, 1073, 1519, 2334, 2564, 3495
CspCI CAANNNNNGTGG 2 cut(s) 679, 714
CviQI GTAC 6 cut(s) 1061, 1073, 1519, 2334, 2564, 3495
DraIII CACNNNGTG 3 cut(s) 1162, 2600, 3747
DrdI GACNNNNNNGTC 1 cut(s) 4239
DseDI GACNNNNNNGTC 1 cut(s) 4239
EaeI YGGCCR 4 cut(s) 297, 687, 3067, 3233
Eam1104I CTCTTC 6 cut(s) 197, 2403, 2856, 3281, 3307, 4674
EarI CTCTTC 6 cut(s) 197, 2403, 2856, 3281, 3307, 4674
EciI GGCGGA 1 cut(s) 2836
Ecl136II GAGCTC 1 cut(s) 3449
Eco130I CCWWGG 4 cut(s) 1985, 2980, 3042, 4150
Eco24I GRGCYC 1 cut(s) 3451
Eco32I GATATC 2 cut(s) 666, 3105
Eco47I GGWCC 7 cut(s) 440, 1769, 1859, 2965, 4232, 4297, 4526
Eco53kI GAGCTC 1 cut(s) 3449
Eco57I CTGAAG 1 cut(s) 495
Eco72I CACGTG 1 cut(s) 3744
Eco88I CYCGRG 1 cut(s) 4775
EcoICRI GAGCTC 1 cut(s) 3449
EcoO109I RGGNCCY 3 cut(s) 162, 4297, 4526
EcoRI GAATTC 2 cut(s) 1202, 4505
EcoRII CCWGG 8 cut(s) 129, 730, 2103, 2723, 3197, 3235, 3306, 4626
EcoRV GATATC 2 cut(s) 666, 3105
EcoT14I CCWWGG 4 cut(s) 1985, 2980, 3042, 4150
EcoT22I ATGCAT 2 cut(s) 654, 2755
EcoT38I GRGCYC 1 cut(s) 3451
ErhI CCWWGG 4 cut(s) 1985, 2980, 3042, 4150
Esp3I CGTCTC 3 cut(s) 12, 1905, 3645
FalI AAGNNNNNCTT 4 cut(s) 1033, 1065, 4314, 4346
FaqI GGGAC 3 cut(s) 453, 893, 4310
FauI CCCGC 1 cut(s) 47
FbaI TGATCA 1 cut(s) 3738
FblI GTMKAC 1 cut(s) 4706
FriOI GRGCYC 1 cut(s) 3451
GlaI GCGC 3 cut(s) 417, 3913, 4413
GsaI CCCAGC 1 cut(s) 2732
GsuI CTGGAG 4 cut(s) 507, 1607, 2126, 3860
HaeII RGCGCY 1 cut(s) 419
HapII CCGG 6 cut(s) 147, 1446, 2146, 2490, 3011, 3889
HgaI GACGC 3 cut(s) 54, 3662, 4050
HhaI GCGC 3 cut(s) 418, 3914, 4414
Hin1I GRCGYC 1 cut(s) 65
Hin6I GCGC 3 cut(s) 416, 3912, 4412
HinP1I GCGC 3 cut(s) 416, 3912, 4412
HincII GTYRAC 4 cut(s) 2029, 4093, 4462, 4707
HindII GTYRAC 4 cut(s) 2029, 4093, 4462, 4707
HindIII AAGCTT 4 cut(s) 233, 240, 1385, 3962
HpaII CCGG 6 cut(s) 147, 1446, 2146, 2490, 3011, 3889
HphI GGTGA 7 cut(s) 385, 673, 1806, 1940, 2612, 2750, 2996
Hpy99I CGWCG 3 cut(s) 70, 82, 119
HpyCH4IV ACGT 5 cut(s) 114, 906, 1517, 1898, 3743
HpySE526I ACGT 5 cut(s) 114, 906, 1517, 1898, 3743
Hsp92I GRCGYC 1 cut(s) 65
HspAI GCGC 3 cut(s) 416, 3912, 4412
KflI GGGWCCC 1 cut(s) 4297
Kpn2I TCCGGA 2 cut(s) 3010, 3888
KpnI GGTACC 1 cut(s) 3498
Ksp22I TGATCA 1 cut(s) 3738
MaeII ACGT 5 cut(s) 114, 906, 1517, 1898, 3743
MaeIII GTNAC 8 cut(s) 391, 1658, 1928, 2006, 2034, 2424, 4382, 4670
MflI RGATCY 7 cut(s) 220, 979, 1115, 2117, 2843, 2938, 4174
MhlI GDGCHC 2 cut(s) 2023, 3451
MlsI TGGCCA 3 cut(s) 689, 3069, 3235
MluNI TGGCCA 3 cut(s) 689, 3069, 3235
MlyI GAGTC 2 cut(s) 3184, 3635
MmeI TCCRAC 6 cut(s) 97, 1025, 1509, 1747, 3526, 3971
Mox20I TGGCCA 3 cut(s) 689, 3069, 3235
Mph1103I ATGCAT 2 cut(s) 654, 2755
MroI TCCGGA 2 cut(s) 3010, 3888
MroXI GAANNNNTTC 2 cut(s) 1290, 2745
MscI TGGCCA 3 cut(s) 689, 3069, 3235
MslI CAYNNNNRTG 4 cut(s) 1000, 2228, 2758, 2893
Msp20I TGGCCA 3 cut(s) 689, 3069, 3235
MspA1I CMGCKG 5 cut(s) 424, 1841, 3784, 3920, 4409
MspCI CTTAAG 1 cut(s) 3442
MspI CCGG 6 cut(s) 147, 1446, 2146, 2490, 3011, 3889
Mva1269I GAATGC 3 cut(s) 1240, 1292, 2110
MvaI CCWGG 8 cut(s) 131, 732, 2105, 2725, 3199, 3237, 3308, 4628
MvnI CGCG 1 cut(s) 4414
NciI CCSGG 2 cut(s) 1446, 2491
NcoI CCATGG 1 cut(s) 1985
NlaIV GGNNCC 8 cut(s) 441, 736, 981, 2940, 3496, 4298, 4299, 4528
NmuCI GTSAC 3 cut(s) 391, 1928, 4382
NsiI ATGCAT 2 cut(s) 654, 2755
NspI RCATGY 5 cut(s) 767, 1655, 1727, 2536, 4705
PaeI GCATGC 1 cut(s) 1655
PaeR7I CTCGAG 1 cut(s) 4775
PagI TCATGA 2 cut(s) 370, 2254
PaqCI CACCTGC 1 cut(s) 649
PciI ACATGT 1 cut(s) 4701
PcsI WCGNNNNNNNCGW 2 cut(s) 74, 111
PctI GAATGC 3 cut(s) 1240, 1292, 2110
PdmI GAANNNNTTC 2 cut(s) 1290, 2745
PflFI GACNNNGTC 1 cut(s) 467
PleI GAGTC 2 cut(s) 3184, 3635
PmaCI CACGTG 1 cut(s) 3744
PmlI CACGTG 1 cut(s) 3744
PpsI GAGTC 2 cut(s) 3184, 3635
Ppu21I YACGTR 2 cut(s) 1518, 3744
PpuMI RGGWCCY 2 cut(s) 4297, 4526
PscI ACATGT 1 cut(s) 4701
PshAI GACNNNNGTC 1 cut(s) 1052
PshBI ATTAAT 1 cut(s) 3927
PsiI TTATAA 1 cut(s) 899
Psp124BI GAGCTC 1 cut(s) 3451
Psp5II RGGWCCY 2 cut(s) 4297, 4526
Psp6I CCWGG 8 cut(s) 129, 730, 2103, 2723, 3197, 3235, 3306, 4626
PspCI CACGTG 1 cut(s) 3744
PspFI CCCAGC 1 cut(s) 2728
PspGI CCWGG 8 cut(s) 129, 730, 2103, 2723, 3197, 3235, 3306, 4626
PspN4I GGNNCC 8 cut(s) 441, 736, 981, 2940, 3496, 4298, 4299, 4528
PspPPI RGGWCCY 2 cut(s) 4297, 4526
PsrI GAACNNNNNNTAC 2 cut(s) 4605, 4637
PstI CTGCAG 2 cut(s) 1618, 4053
PstNI CAGNNNCTG 2 cut(s) 473, 1844
PsuI RGATCY 7 cut(s) 220, 979, 1115, 2117, 2843, 2938, 4174
PsyI GACNNNGTC 1 cut(s) 467
PvuII CAGCTG 3 cut(s) 424, 3784, 3920
RsaI GTAC 6 cut(s) 1062, 1074, 1520, 2335, 2565, 3496
RsaNI GTAC 6 cut(s) 1061, 1073, 1519, 2334, 2564, 3495
RseI CAYNNNNRTG 4 cut(s) 1000, 2228, 2758, 2893
SacI GAGCTC 1 cut(s) 3451
SalI GTCGAC 1 cut(s) 4705
ScaI AGTACT 1 cut(s) 2565
SchI GAGTC 2 cut(s) 3184, 3635
SduI GDGCHC 2 cut(s) 2023, 3451
SfcI CTRYAG 4 cut(s) 1614, 3249, 4049, 4185
Sfr274I CTCGAG 1 cut(s) 4775
SinI GGWCC 7 cut(s) 440, 1769, 1859, 2965, 4232, 4297, 4526
SlaI CTCGAG 1 cut(s) 4775
SmiMI CAYNNNNRTG 4 cut(s) 1000, 2228, 2758, 2893
SmlI CTYRAG 6 cut(s) 483, 912, 1315, 1420, 3442, 4775
SmoI CTYRAG 6 cut(s) 483, 912, 1315, 1420, 3442, 4775
SphI GCATGC 1 cut(s) 1655
SspI AATATT 3 cut(s) 827, 2060, 3216
SstI GAGCTC 1 cut(s) 3451
StyI CCWWGG 4 cut(s) 1985, 2980, 3042, 4150
TaiI ACGT 5 cut(s) 117, 909, 1520, 1901, 3746
TaqI TCGA 9 cut(s) 1273, 1354, 1741, 2290, 2806, 3193, 4333, 4706, 4776
TatI WGTACW 4 cut(s) 1060, 1072, 2333, 2563
TauI GCSGC 7 cut(s) 65, 95, 299, 361, 1844, 2577, 4412
TscAI CASTG 8 cut(s) 267, 820, 1351, 2071, 2078, 2169, 2840, 4497
TseFI GTSAC 3 cut(s) 391, 1928, 4382
Tsp45I GTSAC 3 cut(s) 391, 1928, 4382
TspGWI ACGGA 2 cut(s) 36, 854
TspRI CASTG 8 cut(s) 267, 820, 1351, 2071, 2078, 2169, 2840, 4497
Tth111I GACNNNGTC 1 cut(s) 467
Vha464I CTTAAG 1 cut(s) 3442
VpaK11BI GGWCC 7 cut(s) 440, 1769, 1859, 2965, 4232, 4297, 4526
VspI ATTAAT 1 cut(s) 3927
XbaI TCTAGA 1 cut(s) 2263
XceI RCATGY 5 cut(s) 767, 1655, 1727, 2536, 4705
XhoI CTCGAG 1 cut(s) 4775
XmiI GTMKAC 1 cut(s) 4706
XmnI GAANNNNTTC 2 cut(s) 1290, 2745
ZrmI AGTACT 1 cut(s) 2565
Zsp2I ATGCAT 2 cut(s) 654, 2755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.