Rroxscaffold_1G00009240

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0011193
Physical Location & Seq
Forward (+)
11608699 .. 11620514
11816 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00009240.1

Sequence Viewer

Length: 6309 bp
ATGGGGAAGCAGAAGGGAGACGGAGCTAGGAGCAAGGCCCGCCCTTCAAGTAGCAGTTTGGCGGCGTCGCTGTTGCCGTCGGGTTCTACTGCGGCGGTGGGCTTCGGTGGCTACGTCGGAGGTTCTCGCCTCGATGCTCCTCCTTCCGGCGATGATTCAAGGCCCTTTTTGGACCTTAAGTTTGATTTTAAATCAATTGCTAGGGCAGAAGTAGATAGTGATTTGGCTCTACATTTGAAGAGGCTTGCAAGGAAAGATCCCACCACCAAGCTTAAAGCTTTGGCATCTCTATCCACACTGTTGAAGGAAAAGTCCACGAAGGACATAGTAGCGGCCATTCCACAATGGGGATTTGAATACAAAAGGCTTGTGGTGGACTACAATAGGGATGTGCGGCGAGCAACTCATGACACAATGACCATTCTTGTCACTGCCGTTGGGAGAGATTTAGCGCCACAGCTGAAATCTTTGATGGGACCATGGTGGTTTTCTCAATTTGACCCAGTCTCTGAAGTTTCTCAAGCTGCAAAGCGATCATTTCAGAGTCAAATCAACTGGGGGGCTAGTGCGGACGTCGACACAGCAGGCGGCGCGGGGCTTGCAAGAGGGAGGCCGGAGGCGTCCCGGACCGTTCGGGCACCGTTCGAGGTCGGAGGAGGTCGGGCTTGCCGGTTTCGGGTAGCCACCTCACCTGCAACTGCAGGTTCTGTGCAGCACCGCAAAACCCGTCGTGGCGACTCCACCCGATTGCGGACCCCTCCGCACGACCCCCGGCCTCTCTCCCGGCAAGCTGCGCCGCGCCGTCGCCGCTCGATCGAGGCCGAGCAAAGACGTCCACACTTTTTCCGGGTTGCGGATATAGGGGCGGGTTCTGAAGCGGACGTCCACAACCCGGAAAAAGTGCGGACGTCGCTCCTCCGGCCTCAATCGGGCGTTGACGGCGGCATTTCGTTGCCGGACGACCAGTGCAACGACGTGGAGCAGTTCGAAGCGGCTGGACTCGTCGGCGAAGGGTTTGAAGTCGAGCTCGATGGGCTTCCATGGTTTCCGGCGAGCTCACCGCACATCACCGAGTTTCCGATCATCTTTGCCTTCGCCGCTGGATCGAGGCCGTTCGGGATGTTCGGAGGGTGCGTCCGGCACCCGCAGCACAGCGATTGCCGCCTTCTCGACGCCGGAGGAGGGACGTCTGCACTTTTTCTTGGTTGCGGACGTCCGCTCTCGAACGGTTGTGCAGTCTTTTCAGCTCCAGAAAAGAGACTAGATGCTTTAATTTTATGCACGGCCGAGGTCTTTATTTATTTGGAAGAAAATCTGAGGCTTACACCGCAAAATATGTCCGATAAAGGAACTGCTCTGGATGAATTGCAAGAGATGCATCAGCAGGTGATATCTTCATCATTGCTGGCATTGGCCACAGTTCTTGATGTCTTGGTTTGCTTACAACTAGAAAGACCAGGCACCGAAAACATAGCTGCTCAACCAAAACATGCTTTGAAGGCTAGGGAAACTGCTATTTCTTGTGCCGACAAGTTGTTCACTGCTCATAAATATTTCTTGGACTTCTTGAAATCCCCAAGTCCTGCTATCCGTTCAGCCACATATTCTGTATTGAGTAGTTTTATAAAAAACGTCCCTCAAGCCTTTAATGAAGGAAATATGAAAACTCTTGCTGCTGCACTATTAGGTGGATTTCAGGAGAAGGATCCTGCTTGTCATTCATCAATGTGGGATGCAATTTTACTGTTTTCTAATAAATTTCCTGAAAGTTGGACTTCTGTCAATGTACAGAAAACTGTACTGAATCGGTTTTGGGATTTCCTTAGAAATAGATATTGCAATGGAGTGGATTCCATCAGTAATTTTCGAGCAACTCTTGTTAAAAATGTTCTGGTAAAGCTTTTGTGGCAAGACTACATTTTCTCTAGTAGCTCAAGAAAGGAGGAGAAAACATCCCCCGGATTATCAGTAGATTCATGTGAAAGTGATTTAGCTTCCAACAAGAAGACAGTTGAAACATTGAATATCACGTACCCAATGAGCTACTTTAGAGAGTTGGCAAATTGCATTGTTGGGGTTCTTTCAGGCATTGATTTGCTGGAGCATGATTTGCTCTCAGCTTTTGCTGCAGAATTTCAGGAGAATTGTCAGAGTTTCTTTCAGCATGCTAGTAACTTGGAAAGAGAAAGTGAGTTTGCGGAGCGAGTTATACAGTTTATATCATTACTTGGAGAACATGCAATGCAGAATGGTCGAGGTTGGCCTTTGGCTTCTCTAGTTGGACCAATGTTGGCTAATTCCTTTGCATTGATGAGATCACATGATTCACCAAGTTGTGTGAAGATTCTAGCAGTGGCTGTTTCTGTATTTGGACCTCACAAGATAGTCCACGAACTTCTTATTCACAATACGTCTCCACATAGTTATTCTCTTGATGAGGGTGATAAAGCATTAGAGGAAGATAGATTCCTTCAAATGTTCAATGGAACTTTTGTTCCATGGTGTCTTAGTGGGAATAGTTACTCTTTAAGTGCTCGTCTTGACCTGTTACTTGCATTCCTTGATGATGAATATTTCTCTGAGCAGTGGGATAGTGTTATCAGATATGCCACTAACCTGGAGCATTCTGGATCAGCACCCTGCTCTTTAGATTCTGACCGGATAACTATCTTAGCAATGCTTTTACAGAAAGTAAGAAACAGAATCACTGACACAAAGGTAGGAGTTTCCATCAGCACAAAAATGGGCAACCCAGATCATTGGCATCATGAGCTTCTAGAATCAACTGCTGTTGCTGTTGCTCAATCCTCTCCTCCTCTCGGAGCTTCTAATTCTCAATTCTTGTGTACTGTTGTTGCTGGTTCAACAAAGACCAATCAAATTTCTCTGGTGTCAAGAAATACATTGATCCTGATATTTGAAGAGGTTTTCAAGAAGTTACTTTCTTTTATTATGGCGTCCTCTTTTACTTGGGTTAGGGATGCAGGTTCTCTATTGACTGCTGGGGCAAACACTATTGGGCCAGAATTTGAAAATTCTGTGGACATGTATGAGATGGCTCAATTTGCTCTTGAAGTACTTGATGGCGGCTTATATTCATTAAAGACACTTGGTGAAGAAAGTGGGTTGAGTTCAGTTATTTTAGCTGCAATTTTTCTGATTGACTGGGAGTTTCTGGTATTACTGACAACGATAGATGATGCACCTGATGATAAATCAAGGGAAAAATTGAAGGCCAGGCTGGGATTTGGTGAATCCTTTCATGCATTTAGATGTAAGATAAGTAATCAATTCTGGAAAACACTCAGCTTACACAATCGAAAGGCACTTGGAAAAAATTTGATTCAGTGTATGAGATCTGCGATCTTCAATGAAGAGGAATTAGACACAGAGAAGTTCACATCTCTGTGCTGCTTGTGGATGCTTGAAATTCTGGACTGTCTCTCTCAGGATCCCTATGAGGAACAAAATCTGCTGGACCAACTTCTCTGCCAAGGTGATAGATGGCCTTTGTGGATAGTTCCGGATTTTAGCAGGCCAGAGGGAATAGTTGCCAAGGACTTCTCTATCCAAGACTTTGGTCACCGCAAGTTTATTTCATTTATTGACAAGATGATATCAGAAATTGGGATTGATAGAGTTGTTGGGGGTTATGCTAGATATACTCTCCCTCCCTCTGTGGAAGCAACAAATGAAAAGCCGACTCGATCCTGGCTTGCTGCTGAAATTTTATGCTCGTGGAAATGGCCAGGAGGAAGTGCTGTAGCTTCATTCTTACCTTCATTGAGTGCCTATGCGAAGAGTAAAAATTTCTCTTCCCAGGAGAGCTTGCTAGATTTTATTTTCAACTTTCTACTTGATGGCACACTTGTTCAGGGAGGATGTGCTGTACAGAATTTTGTCTATCTGTGTCCTACTTCAAGTGATGAAGTGGAGGATATTGAAGAACCATTCTTAAGAGCTCTTGTAGCTTTTCTTTTAACTTTGTTTAATGATAAAATATGGGGTACTGAGAAAGCTATGGAGCTGTTTGCATTGCTTGTAAATAAACTTTCTGTTGGAGAAGCAACAAATGCAAACTGTTTGAGGATTCTTCCTCTGATTGTTAATGTTCTTATTCAACCATTAAGTCAAAGAAGCATCAGATGTAATGACTCTAGTGGAGACGCTCAACATGATTCTTCTGGGGAAAATCATGTGCAAGATGTTATTGAAGGCTGGCTACAAAAAGCTCTATCATTTCCGCCTTTGTTCACGTGGTGGCAAACAGGAGAAGATATGGAAGATTGGATGCAGCTGGTTATTTCTTGTTATCCCATTAGTGCAGTAGAGGACATACAAACACCGAAGCTGGAGAGAAAGATCAGCTCGGTAGAGAGAAAGCTCCTACTTGAATTATTCCGGAAGCAGAGACATGGTGATGGCACACCAGCTGTAATTAATCAGCTTCCAGTTGTGCAGATGTTGCTATCAAAGCTTATGGTCGTTTCAGTTGGTTATTGTTGGAAAGAATTTGATGAAGAAGATTGGGAGTTTGTGTTGTCTCAGATAAGAGGCTGGCTGCAGTCTGTTGTTGTAATGATGGAGGAAATTGCTGAAAATGTCAACGACACTATCACTAGCAGCTTTACTTCTGATAACTTGGATGATGTCGTTGATAACCTTGGCAAAATTGTTTTCGTTTCAGATCCCTTTCCTATAGACATTGCCAAAAATGCCCTTTTATCATTTTCTCTGTCCTGTGGACCTTTTGGTCGCCAACAAGCAGAAGATGCAGATAACTTAAATCCTGTGAGAACAGAAAGATGGGACCCCATCAAAAATAGAATTTTAGAAGGCATCCTTCGATTGTTGTTCTGCACGGGTATTGCTGAGGCCATTGCAAGCTCCTGTTGTCACGAGGCTGCATTTATTGTATCAGCGTCGCGATTTGAACATTCCTATTTCTGGGAATTGGTGGCCTCAAATGTGGTCAACTCATCAACAGATGCCATAGATAGAGCAGTGAAGTCAGTTGAATTCTGGGGGCTGAGCAAAGGACCCATTAGCTCTTTGTATGCAATCCTTTTTTCTGCCAAAACAGTTCCTCTGTTGCAGTTCGCTGCTTACTTTATTCTTTCAACTGAACTTGTTTTACGCCTGGCTATTGTTGAAGAAGACAAAAGTTACTTGGATGGCGTTAGTAACAATGAAGAGGTTTCATCCTCTCTTGACATGTCGACAGAAACAGATATTCATTTGAGAGCGGAAATATCTTGCATGATTGAAAAGTTACCTAGTAATGTTCTCGAGATGGATTTAGTGGCAGATCAAAGGGTACATGTTTTTCTTGCGTGGTCCTTGTTGTTATCTCATTTAGGGTCATTGCCATCATCATCGCCTGCAAGGGAGAGACTGGTCCAGTATGTACAAGATTCTGCTAATCCAGTAATATTAGGTTGCCTCTTCCAGCATATTCCTCTAGAACTGTGGATTCTTAAGAAGAAAGACGAAGAGCTTCCTGCTGGCATAGCAGAAGCTGCAGCTGCAGCAACACGTTCCATTAGAACTGGTTCCTTATTGTTTTCTGTACAGTCTCTCTGGCCTGTTGAACCACTGAAAATGGCTTCGCTTGCTGGAGCAATGTTTGGTCTGATGCTCCACATTCTTCCCGCCTATGTAAGACAGTGGTCCAATGATTTACGTGACCGCTCTACATTATCAGGAATTGAATCGTTCACAAGAGCATGGTGCAGTCCACATCTCATCGCTGGTGAATTAAGTCACATTAAAAAGGAAGAAATTGCTGACGAGAATTTTACCATTGCTGTAAGCAAATCGGCAAATGAGGTTGTTGCTACTTATACAAAGGATGAGACTGCAATGAATCTAGTAATCCGTCTTCCTTCATCTTACCCATTACGGCCTGTTGATGTTGATTGTACAAGGAGTCTAGGTATTAGTGAAGCGAAGCAGAGGAAATGGTCAATGTCTATGACATCATTTGTACGGAATCAGAATGGAGCTTTAGCAGAAGCTATCCGGATATGGAAGCGCAACTTTGACAAGGAGTTTGAAGGTGTCGAGGAGTGTCCTATTTGTTACAGTGTCATCCATACCGTCAACCACGCCCTTCCACGTCTAGCATGCAAGACCTGCAAGCACAAGTTCCATTCGGCCTGCCTCTATAAGTGTTGCACCCTTAAAGATTATACAGTGAGCAAAAGGATCTTGATCCAGTCAAATAGTAAGACATACAACCAGAATTCAGACCTCTATTGTCGCACAGAAGAATTAAATGCTGATTTCGCCGCAAGAGATGGAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

2102

Amino Acids

233.29

Weight (kDa)

5.58

Isoelectric Point (pI)

46.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ltn1_1st PF22958 72 - 182 1.4e-37 E3 ubiquitin-protein ligase listerin, N-terminal domain
Ltn1_1st PF22958 412 - 615 6.5e-37 E3 ubiquitin-protein ligase listerin, N-terminal domain
LTN1_E3_ligase_6th PF22999 1681 - 1924 3.6e-27 LTN1 family HEAT repeat region
UBC_like PF23009 1934 - 2017 9.8e-30 Ubiquitin conjugating domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G58410 AT5G58410
fragaria_vesca FvH4_3g39950 FvH4_3g39950 FvH4_3g39950
malus_domestica MD03G1058100.v1.1
prunus_persica Prupe.6G045900_v2.0.a1 Prupe.6G045900_v2.0.a1 Prupe.6G045900_v2.0.a1
pyrus_communis pycom03g04640
rosa_chinensis RchiOBHm_Chr5g0071961
rosa_laevigata RLG00000036266
rosa_multiflora Rmu_sc0012806.1_g000004
rosa_roxburghii Rroxscaffold_1G00009240
rosa_rugosa Rorug05G0415700
rosa_samantha Rh5AG471700 Rh5BG491700 Rh5CG515500 Rh5DG503100
rosa_wichuraiana Rw5G043850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1625
AarI CACCTGC 2 cut(s) 700, 1375
AasI GACNNNNNNGTC 1 cut(s) 4752
AatII GACGTC 6 cut(s) 576, 835, 885, 911, 1190, 1216
Acc36I ACCTGC 5 cut(s) 692, 700, 1375, 2975, 6143
AccB1I GGYRCC 3 cut(s) 637, 1140, 1460
AccBSI CCGCTC 4 cut(s) 810, 1219, 5246, 5691
AccI GTMKAC 2 cut(s) 576, 5219
AccII CGCG 3 cut(s) 593, 799, 4927
AccIII TCCGGA 3 cut(s) 3523, 4401, 6021
AcoI YGGCCR 4 cut(s) 333, 1284, 1413, 3746
AcuI CTGAAG 2 cut(s) 531, 894
AcvI CACGTG 1 cut(s) 4257
AdeI CACNNNGTG 2 cut(s) 3113, 4260
AflII CTTAAG 3 cut(s) 176, 3955, 5477
AflIII ACRYGT 4 cut(s) 3045, 5214, 5320, 5534
AjiI CACGTC 2 cut(s) 976, 6119
AjnI CCWGG 7 cut(s) 1456, 2616, 3236, 3710, 3748, 3819, 5139
AloI GAACNNNNNNTCC 4 cut(s) 2478, 2479, 2510, 2511
Alw21I GWGCWC 4 cut(s) 1029, 1058, 2536, 3964
AlwNI CAGNNNCTG 4 cut(s) 509, 707, 2357, 5519
Ama87I CYCGRG 1 cut(s) 5288
Aor13HI TCCGGA 3 cut(s) 3523, 4401, 6021
ArsI GACNNNNNNTTYG 4 cut(s) 164, 196, 6035, 6067
AseI ATTAAT 1 cut(s) 4440
Asp700I GAANNNNTTC 3 cut(s) 3258, 5496, 5551
AspLEI GCGC 5 cut(s) 454, 593, 796, 801, 6036
AsuC2I CCSGG 6 cut(s) 625, 772, 784, 848, 893, 1959
AsuII TTCGAA 1 cut(s) 987
AvaI CYCGRG 1 cut(s) 5288
BaeGI GKGCMC 1 cut(s) 640
BalI TGGCCA 2 cut(s) 1415, 3748
BamHI GGATCC 2 cut(s) 1705, 3451
BanI GGYRCC 3 cut(s) 637, 1140, 1460
BanII GRGCYC 3 cut(s) 1029, 1058, 3964
BarI GAAGNNNNNNTAC 4 cut(s) 4206, 4238, 5865, 5897
BauI CACGAG 2 cut(s) 3736, 4898
BbrPI CACGTG 1 cut(s) 4257
BbsI GAAGAC 3 cut(s) 2012, 5163, 5873
Bbv12I GWGCWC 4 cut(s) 1029, 1058, 2536, 3964
BbvCI CCTCAGC 1 cut(s) 4872
BceAI ACGGC 7 cut(s) 61, 419, 786, 955, 1096, 1299, 5918
BcgI CGANNNNNNTGC 2 cut(s) 2234, 2268
BciT130I CCWGG 7 cut(s) 1458, 2618, 3238, 3712, 3750, 3821, 5141
BcnI CCSGG 6 cut(s) 625, 772, 784, 848, 893, 1959
BfmI CTRYAG 7 cut(s) 699, 2127, 3762, 4562, 4698, 5520, 5526
BfoI RGCGCY 1 cut(s) 455
BfrI CTTAAG 3 cut(s) 176, 3955, 5477
BfuAI ACCTGC 5 cut(s) 692, 700, 1375, 2975, 6143
BglII AGATCT 1 cut(s) 3356
BlpI GCTNAGC 1 cut(s) 5030
BmcAI AGTACT 1 cut(s) 3078
BmeT110I CYCGRG 1 cut(s) 5288
BmgBI CACGTC 2 cut(s) 976, 6119
BmrI ACTGGG 3 cut(s) 497, 565, 3175
BmuI ACTGGG 3 cut(s) 497, 565, 3175
BoxI GACNNNNGTC 3 cut(s) 1778, 3579, 5668
BpiI GAAGAC 3 cut(s) 2012, 5163, 5873
BpmI CTGGAG 5 cut(s) 1233, 2120, 2639, 4373, 5637
Bpu10I CCTNAGC 1 cut(s) 4872
Bpu1102I GCTNAGC 1 cut(s) 5030
Bpu14I TTCGAA 1 cut(s) 987
BpuEI CTTGAG 3 cut(s) 504, 1623, 1918
BpuMI CCSGG 6 cut(s) 625, 772, 784, 848, 893, 1959
BsaAI YACGTR 3 cut(s) 2031, 4257, 5684
BsaBI GATNNNNATC 2 cut(s) 2666, 6212
BsaWI WCCGGW 4 cut(s) 2658, 3523, 4401, 6021
BsaXI ACNNNNNCTCC 8 cut(s) 1119, 1149, 3655, 3685, 4523, 4553, 5920, 5950
Bse118I RCCGGY 1 cut(s) 669
Bse8I GATNNNNATC 2 cut(s) 2666, 6212
BseAI TCCGGA 3 cut(s) 3523, 4401, 6021
BseBI CCWGG 7 cut(s) 1458, 2618, 3238, 3712, 3750, 3821, 5141
BseJI GATNNNNATC 2 cut(s) 2666, 6212
BseMII CTCAG 9 cut(s) 1307, 2130, 2571, 3319, 3461, 4002, 4559, 4863, 5021
BseRI GAGGAG 8 cut(s) 129, 669, 905, 1194, 1958, 2802, 2805, 6080
BseSI GKGCMC 1 cut(s) 640
BseX3I CGGCCG 1 cut(s) 1284
BseYI CCCAGC 2 cut(s) 3002, 3241
BsgI GTGCAG 8 cut(s) 731, 1176, 1254, 1662, 4344, 4478, 4843, 5752
Bsh1236I CGCG 3 cut(s) 593, 799, 4927
Bsh1285I CGRYCG 2 cut(s) 816, 1287
BshNI GGYRCC 3 cut(s) 637, 1140, 1460
BsiEI CGRYCG 2 cut(s) 816, 1287
BsiHKAI GWGCWC 4 cut(s) 1029, 1058, 2536, 3964
BsiHKCI CYCGRG 1 cut(s) 5288
BslFI GGGAC 5 cut(s) 489, 607, 1198, 1619, 4823
BsmBI CGTCTC 3 cut(s) 12, 2418, 4158
BsmFI GGGAC 5 cut(s) 489, 607, 1198, 1619, 4823
BsmI GAATGC 2 cut(s) 2555, 2623
BsoBI CYCGRG 1 cut(s) 5288
Bsp119I TTCGAA 1 cut(s) 987
Bsp1286I GDGCHC 5 cut(s) 640, 1029, 1058, 2536, 3964
Bsp13I TCCGGA 3 cut(s) 3523, 4401, 6021
Bsp1407I TGTACA 5 cut(s) 1786, 3889, 5407, 5569, 5921
Bsp1720I GCTNAGC 1 cut(s) 5030
Bsp19I CCATGG 3 cut(s) 479, 1040, 2498
Bsp68I TCGCGA 1 cut(s) 4927
BspCNI CTCAG 9 cut(s) 1308, 2129, 2572, 3318, 3460, 4003, 4558, 4864, 5022
BspEI TCCGGA 3 cut(s) 3523, 4401, 6021
BspFNI CGCG 3 cut(s) 593, 799, 4927
BspHI TCATGA 2 cut(s) 406, 2767
BspMAI CTGCAG 5 cut(s) 703, 2131, 4566, 5524, 5530
BspMI ACCTGC 5 cut(s) 692, 700, 1375, 2975, 6143
BspQI GCTCTTC 1 cut(s) 5487
BspT104I TTCGAA 1 cut(s) 987
BspT107I GGYRCC 3 cut(s) 637, 1140, 1460
BspTI CTTAAG 3 cut(s) 176, 3955, 5477
BsrBI CCGCTC 4 cut(s) 810, 1219, 5246, 5691
BsrFI RCCGGY 1 cut(s) 669
BsrGI TGTACA 5 cut(s) 1786, 3889, 5407, 5569, 5921
BssAI RCCGGY 1 cut(s) 669
BssSI CACGAG 2 cut(s) 3736, 4898
BssT1I CCWWGG 6 cut(s) 479, 1040, 2498, 3493, 3555, 4663
Bst2BI CACGAG 2 cut(s) 3736, 4898
Bst2UI CCWGG 7 cut(s) 1458, 2618, 3238, 3712, 3750, 3821, 5141
Bst6I CTCTTC 8 cut(s) 233, 2916, 3369, 3794, 3820, 5187, 5450, 5487
BstAFI CTTAAG 3 cut(s) 176, 3955, 5477
BstAPI GCANNNNNTGC 7 cut(s) 1375, 2110, 4073, 4465, 4772, 5519, 6135
BstAUI TGTACA 5 cut(s) 1786, 3889, 5407, 5569, 5921
BstBAI YACGTR 3 cut(s) 2031, 4257, 5684
BstBI TTCGAA 1 cut(s) 987
BstDSI CCRYGG 3 cut(s) 479, 1040, 2498
BstEII GGTNACC 1 cut(s) 3581
BstFNI CGCG 3 cut(s) 593, 799, 4927
BstH2I RGCGCY 1 cut(s) 455
BstHHI GCGC 5 cut(s) 454, 593, 796, 801, 6036
BstMCI CGRYCG 2 cut(s) 816, 1287
BstNI CCWGG 7 cut(s) 1458, 2618, 3238, 3712, 3750, 3821, 5141
BstNSI RCATGY 7 cut(s) 1493, 2168, 2240, 3049, 5218, 5324, 6129
BstPAI GACNNNNGTC 3 cut(s) 1778, 3579, 5668
BstPI GGTNACC 1 cut(s) 3581
BstSFI CTRYAG 7 cut(s) 699, 2127, 3762, 4562, 4698, 5520, 5526
BstSLI GKGCMC 1 cut(s) 640
BstUI CGCG 3 cut(s) 593, 799, 4927
BstV2I GAAGAC 3 cut(s) 2012, 5163, 5873
BstX2I RGATCY 6 cut(s) 256, 1705, 3356, 3451, 4687, 6207
BstXI CCANNNNNNTGG 3 cut(s) 2617, 2760, 3578
BstYI RGATCY 6 cut(s) 256, 1705, 3356, 3451, 4687, 6207
BstZI CGGCCG 1 cut(s) 1284
BtgI CCRYGG 3 cut(s) 479, 1040, 2498
BtgZI GCGATG 3 cut(s) 165, 5361, 5731
BtrI CACGTC 2 cut(s) 976, 6119
BtsI GCAGTG 5 cut(s) 429, 1539, 2358, 2591, 5010
BtuMI TCGCGA 1 cut(s) 4927
BveI ACCTGC 5 cut(s) 692, 700, 1375, 2975, 6143
CaiI CAGNNNCTG 4 cut(s) 509, 707, 2357, 5519
CciI TCATGA 2 cut(s) 406, 2767
CfoI GCGC 5 cut(s) 454, 593, 796, 801, 6036
Cfr10I RCCGGY 1 cut(s) 669
CpoI CGGWCCG 1 cut(s) 627
CseI GACGC 7 cut(s) 54, 609, 1123, 1181, 2946, 4175, 4911
CspCI CAANNNNNGTGG 2 cut(s) 1405, 1440
CspI CGGWCCG 1 cut(s) 627
DraI TTTAAA 1 cut(s) 190
DraIII CACNNNGTG 2 cut(s) 3113, 4260
DrdI GACNNNNNNGTC 1 cut(s) 4752
DseDI GACNNNNNNGTC 1 cut(s) 4752
EaeI YGGCCR 4 cut(s) 333, 1284, 1413, 3746
EagI CGGCCG 1 cut(s) 1284
Eam1104I CTCTTC 8 cut(s) 233, 2916, 3369, 3794, 3820, 5187, 5450, 5487
EarI CTCTTC 8 cut(s) 233, 2916, 3369, 3794, 3820, 5187, 5450, 5487
EciI GGCGGA 1 cut(s) 4233
Ecl136II GAGCTC 3 cut(s) 1027, 1056, 3962
EclXI CGGCCG 1 cut(s) 1284
Eco130I CCWWGG 6 cut(s) 479, 1040, 2498, 3493, 3555, 4663
Eco24I GRGCYC 3 cut(s) 1029, 1058, 3964
Eco32I GATATC 2 cut(s) 1392, 3618
Eco52I CGGCCG 1 cut(s) 1284
Eco53kI GAGCTC 3 cut(s) 1027, 1056, 3962
Eco57I CTGAAG 2 cut(s) 531, 894
Eco72I CACGTG 1 cut(s) 4257
Eco88I CYCGRG 1 cut(s) 5288
Eco91I GGTNACC 1 cut(s) 3581
EcoICRI GAGCTC 3 cut(s) 1027, 1056, 3962
EcoO109I RGGNCCY 3 cut(s) 162, 4810, 5039
EcoO65I GGTNACC 1 cut(s) 3581
EcoRI GAATTC 2 cut(s) 5018, 6244
EcoRII CCWGG 7 cut(s) 1456, 2616, 3236, 3710, 3748, 3819, 5139
EcoRV GATATC 2 cut(s) 1392, 3618
EcoT14I CCWWGG 6 cut(s) 479, 1040, 2498, 3493, 3555, 4663
EcoT22I ATGCAT 2 cut(s) 1380, 3268
EcoT38I GRGCYC 3 cut(s) 1029, 1058, 3964
ErhI CCWWGG 6 cut(s) 479, 1040, 2498, 3493, 3555, 4663
Esp3I CGTCTC 3 cut(s) 12, 2418, 4158
FalI AAGNNNNNCTT 6 cut(s) 1759, 1791, 4827, 4859, 6023, 6055
FaqI GGGAC 5 cut(s) 489, 607, 1198, 1619, 4823
FauI CCCGC 5 cut(s) 47, 586, 859, 1152, 5659
FblI GTMKAC 2 cut(s) 576, 5219
FriOI GRGCYC 3 cut(s) 1029, 1058, 3964
GlaI GCGC 5 cut(s) 453, 592, 795, 800, 6035
GsaI CCCAGC 2 cut(s) 3006, 3245
GsuI CTGGAG 5 cut(s) 1233, 2120, 2639, 4373, 5637
HaeII RGCGCY 1 cut(s) 455
HgaI GACGC 7 cut(s) 54, 609, 1123, 1181, 2946, 4175, 4911
HhaI GCGC 5 cut(s) 454, 593, 796, 801, 6036
Hin6I GCGC 5 cut(s) 452, 591, 794, 799, 6034
HinP1I GCGC 5 cut(s) 452, 591, 794, 799, 6034
HincII GTYRAC 6 cut(s) 577, 937, 4606, 4975, 5220, 6103
HindII GTYRAC 6 cut(s) 577, 937, 4606, 4975, 5220, 6103
HindIII AAGCTT 4 cut(s) 269, 276, 1898, 4475
HspAI GCGC 5 cut(s) 452, 591, 794, 799, 6034
KflI GGGWCCC 1 cut(s) 4810
Kpn2I TCCGGA 3 cut(s) 3523, 4401, 6021
LguI GCTCTTC 1 cut(s) 5487
MbiI CCGCTC 4 cut(s) 810, 1219, 5246, 5691
MfeI CAATTG 1 cut(s) 195
MflI RGATCY 6 cut(s) 256, 1705, 3356, 3451, 4687, 6207
MhlI GDGCHC 5 cut(s) 640, 1029, 1058, 2536, 3964
MlsI TGGCCA 2 cut(s) 1415, 3748
MluNI TGGCCA 2 cut(s) 1415, 3748
MlyI GAGTC 6 cut(s) 553, 731, 993, 3697, 4148, 5938
MmeI TCCRAC 7 cut(s) 97, 631, 1751, 2022, 2260, 4039, 4484
Mox20I TGGCCA 2 cut(s) 1415, 3748
Mph1103I ATGCAT 2 cut(s) 1380, 3268
MroI TCCGGA 3 cut(s) 3523, 4401, 6021
MroXI GAANNNNTTC 3 cut(s) 3258, 5496, 5551
MscI TGGCCA 2 cut(s) 1415, 3748
MslI CAYNNNNRTG 5 cut(s) 1726, 2741, 3271, 3406, 4419
Msp20I TGGCCA 2 cut(s) 1415, 3748
MspA1I CMGCKG 5 cut(s) 460, 1100, 4297, 4433, 5525
MspCI CTTAAG 3 cut(s) 176, 3955, 5477
MteI GCGCNGCGC 1 cut(s) 797
MunI CAATTG 1 cut(s) 195
Mva1269I GAATGC 2 cut(s) 2555, 2623
MvaI CCWGG 7 cut(s) 1458, 2618, 3238, 3712, 3750, 3821, 5141
MvnI CGCG 3 cut(s) 593, 799, 4927
NciI CCSGG 6 cut(s) 625, 772, 784, 848, 893, 1959
NcoI CCATGG 3 cut(s) 479, 1040, 2498
NmeAIII GCCGAG 2 cut(s) 847, 1312
NmuCI GTSAC 5 cut(s) 427, 3581, 4895, 5684, 5762
NruI TCGCGA 1 cut(s) 4927
NsiI ATGCAT 2 cut(s) 1380, 3268
NspI RCATGY 7 cut(s) 1493, 2168, 2240, 3049, 5218, 5324, 6129
NspV TTCGAA 1 cut(s) 987
PaeI GCATGC 2 cut(s) 2168, 6129
PaeR7I CTCGAG 1 cut(s) 5288
PagI TCATGA 2 cut(s) 406, 2767
PaqCI CACCTGC 2 cut(s) 700, 1375
PciI ACATGT 3 cut(s) 3045, 5214, 5320
PciSI GCTCTTC 1 cut(s) 5487
PcsI WCGNNNNNNNCGW 3 cut(s) 74, 111, 1131
PctI GAATGC 2 cut(s) 2555, 2623
PdmI GAANNNNTTC 3 cut(s) 3258, 5496, 5551
PflFI GACNNNGTC 1 cut(s) 503
PfoI TCCNGGA 1 cut(s) 623
Ple19I CGATCG 1 cut(s) 816
PleI GAGTC 6 cut(s) 552, 731, 993, 3697, 4148, 5937
PmaCI CACGTG 1 cut(s) 4257
PmlI CACGTG 1 cut(s) 4257
PpsI GAGTC 6 cut(s) 552, 731, 993, 3697, 4148, 5937
Ppu21I YACGTR 3 cut(s) 2031, 4257, 5684
PpuMI RGGWCCY 2 cut(s) 4810, 5039
PscI ACATGT 3 cut(s) 3045, 5214, 5320
PshAI GACNNNNGTC 3 cut(s) 1778, 3579, 5668
PshBI ATTAAT 1 cut(s) 4440
PsiI TTATAA 1 cut(s) 1625
Psp124BI GAGCTC 3 cut(s) 1029, 1058, 3964
Psp5II RGGWCCY 2 cut(s) 4810, 5039
Psp6I CCWGG 7 cut(s) 1456, 2616, 3236, 3710, 3748, 3819, 5139
PspCI CACGTG 1 cut(s) 4257
PspEI GGTNACC 1 cut(s) 3581
PspFI CCCAGC 2 cut(s) 3002, 3241
PspGI CCWGG 7 cut(s) 1456, 2616, 3236, 3710, 3748, 3819, 5139
PspPPI RGGWCCY 2 cut(s) 4810, 5039
PsrI GAACNNNNNNTAC 2 cut(s) 5118, 5150
PstI CTGCAG 5 cut(s) 703, 2131, 4566, 5524, 5530
PstNI CAGNNNCTG 4 cut(s) 509, 707, 2357, 5519
PsuI RGATCY 6 cut(s) 256, 1705, 3356, 3451, 4687, 6207
PsyI GACNNNGTC 1 cut(s) 503
PvuI CGATCG 1 cut(s) 816
PvuII CAGCTG 4 cut(s) 460, 4297, 4433, 5525
RruI TCGCGA 1 cut(s) 4927
RseI CAYNNNNRTG 5 cut(s) 1726, 2741, 3271, 3406, 4419
Rsr2I CGGWCCG 1 cut(s) 627
RsrII CGGWCCG 1 cut(s) 627
SacI GAGCTC 3 cut(s) 1029, 1058, 3964
SalI GTCGAC 2 cut(s) 575, 5218
SapI GCTCTTC 1 cut(s) 5487
ScaI AGTACT 1 cut(s) 3078
SchI GAGTC 6 cut(s) 553, 731, 993, 3697, 4148, 5938
SduI GDGCHC 5 cut(s) 640, 1029, 1058, 2536, 3964
SfcI CTRYAG 7 cut(s) 699, 2127, 3762, 4562, 4698, 5520, 5526
Sfr274I CTCGAG 1 cut(s) 5288
SfuI TTCGAA 1 cut(s) 987
SlaI CTCGAG 1 cut(s) 5288
SmiMI CAYNNNNRTG 5 cut(s) 1726, 2741, 3271, 3406, 4419
SmlI CTYRAG 7 cut(s) 176, 519, 1638, 1933, 3955, 5288, 5477
SmoI CTYRAG 7 cut(s) 176, 519, 1638, 1933, 3955, 5288, 5477
SphI GCATGC 2 cut(s) 2168, 6129
SspI AATATT 3 cut(s) 1553, 2573, 5433
SstI GAGCTC 3 cut(s) 1029, 1058, 3964
StyI CCWWGG 6 cut(s) 479, 1040, 2498, 3493, 3555, 4663
TatI WGTACW 8 cut(s) 1786, 1798, 2846, 3076, 3889, 5407, 5569, 5921
TseFI GTSAC 5 cut(s) 427, 3581, 4895, 5684, 5762
Tsp45I GTSAC 5 cut(s) 427, 3581, 4895, 5684, 5762
TspGWI ACGGA 4 cut(s) 36, 1580, 5867, 6004
Tth111I GACNNNGTC 1 cut(s) 503
Vha464I CTTAAG 3 cut(s) 176, 3955, 5477
VspI ATTAAT 1 cut(s) 4440
XbaI TCTAGA 2 cut(s) 2776, 5461
XceI RCATGY 7 cut(s) 1493, 2168, 2240, 3049, 5218, 5324, 6129
XhoI CTCGAG 1 cut(s) 5288
XmiI GTMKAC 2 cut(s) 576, 5219
XmnI GAANNNNTTC 3 cut(s) 3258, 5496, 5551
ZraI GACGTC 6 cut(s) 574, 833, 883, 909, 1188, 1214
ZrmI AGTACT 1 cut(s) 3078
Zsp2I ATGCAT 2 cut(s) 1380, 3268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.