RLG00000036266

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0011193
Physical Location & Seq
Reverse (-)
78206295 .. 78216646
10352 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036266

Sequence Viewer

Length: 5712 bp
ATGGGGAAGCAGAAGGGCGACGGAGCTAGGAGCAAGGCCCGCCCTTCAAGTAGCAGTTTGGCGGCGTCGCTGTTGCCGTCGGGTTCTACTGCGGCGGCGGGCTTCGGTGGCTACGTCGGAGGCTCTCGCCTCGATGCTCCTCCTTCCGGCGATGATTCAAGGCCCTTTTTGGAAGTAGATAGTGATTTGGCTCTACATTTGAAGAGGCTTGCAAGGAAAGATCCCACCACCAAGCTTAAAGCTTTGGCATCTCTATCCACACTGTTGAAGGAAAAGTCCACAACGGACATAGTAGCGGCCATTCCACAATGGGGATTTGAATACAAAAGGCTTGTGGTGGACTACAATAGGGATGTGCGGCGAGCAACTCATGACACAATGACCATTCTTGTCACCGCCGTTGGGAGAGATTTAGCGCCACAGCTGAAATCTTTGATGGGACCATGGTGGTTTTCTCAATTTGACCCAGTCTCTGAAGTTTCTCAAGCTGCAAAGCGATCATTTCAGGCAGTCTTTTCAGCTCCAGAAAAGAGACTAGATGCTTTAATTTTATGCACGGCAGAGGTTTTTATTTATTTGGAAGAAAATCTGAGGCTTACACCGCAAAATATGTCCGATAAAGGAACTGCTCTGGATGAATTGCAAGAGATGCATCAGCAGGTGATATCTTCATCATTGCTGGCATTGGCCACACTTCTTGATGTCTTGGTTTGCTTACAACTAGAAAGATCAGGCACCGAAAACATAGCTGCTCAACCAAAACATGCTTTGAAGGCTAGGGAAACTGCTATTTCTTGTGCCGACAAGTTGTTCACTGCTCATAAATATTTCTTAGACTTCTTGAAATCCCCAAGTCCTGCTATCCGTTCAGCCACATATTCTGTATTGAGTAGTTTTATAAAAAACGTCCCTCAAGCCTTTAATGAAGGAAATATGAAAACTCTTGCTGCTGCACTATTAGGTGGATTTCAGGAGAAGGATCCTGCTTGTCATTCATCAATGTGGGATGCAATTTTACTGTTTTCTAATAAATTTCCAGAAAGTTGGACTTCTGTCAATGTACAGAAAACTGTACTGAATCGGTTTTGGGATTTCCTTAGAAATAGGTGCTTTGGATCTCAACAGGTTTCTTATCCATCTTTGGTTCTATTCTTACACACTGTGCCATCTAAAGCAGTCGTTGCAGAAACTTTTTTTCTTGAATTCTTCAAGAACCTCTGGGCGGGAAGGAATCCATCGCATTCCTTAAATGCAGATAGAGTGGCATTTTTTCGAGCGTTACAAGAATGCTTTCTTTGGGCATTGCACAATGCCTCAAGATATTGCAATGGAGTGGATTCCATCAGTGATTTTCGAGTAACTCTTGTTAAAAATGTTCTGGTAAAGCTTTTGTGGCAAGACTACATTTTCTCTAGTAGCTCAAGAAAGGAGGAGAAAACATCCCCCAAATTATCAGCAGATTCATGTGAAAGTGATTTAGCTTCCAACAAGAAGACAGTTGAAACATTGAATATCACGTACCCAATGAGCTACTTTAGAGAGTTGGCAAATTGCATTGTTGGGGTTCTTTCAGGCATTGATTTGCTGGAGCATGATTTGCTCTCAGCTTTTGCTGCAGAATTTCAGGAGAATTGTCAGAGTTTCTTTCAGCATGCTAGTAACTTGGAAAGAGAAAGTGAGTTTGCGGAGCGAGTTATACAGTTTATATCATTACTTGGAGAACATGCAATGCAGAATGGTCGAGGTTGGCCTTCGGCTTCTCTAGTTGGACCCATGTTGGCTAATTCCTTTGCATTGATGAGATCACATGATTCACCAAGTTGTGTGAAGATTCTAGCAGTGGCTGTTTCTGTATTTGGACCTCACAAGATAGTTCACGAACTTCTTATTCACAATACGTCTCCACATAGTTATTCTCCTGATGAGGGTGATAAAGCATTAGAGGAAGATAGATTCCTGCAAATGTTCAATGGAACTTTTGTTCCATGGTGTCTTAGTGGGAATAGTTACTCTTTAAGTGCTCGTCTTGACCTGTTACTTGCATTGCTTGATGATGAATATTTCTCTGAGCAGTGGGATAGTGTTATCAGATATGCCACTAACCTGGAGCATTCTGGATCTGCACCCTGCTCTTTAGATTCTGACCGGATAACTATCTTAGCAATGCTTTTACAGAAAGTAAGAAACGGAATCACAAACACAAAGGTAGGAGTTTCCATCAGCACAAAAATGGGCAACCCAGATCATTGGCATCATGAGCTTCTAGAATCAACTGCTGTTGCTGTTGCTCGATCCTCTCCTCCTCTCGGAGCTTCTAATTCTCAATTCTTGTGTACTGTTGTTGGTGGTTCAACAAAGACCAATCAAATTTCTCTGGTGTCAAGAAATACATTGATCCTGATATTTGAAGAGGTTTTCAAGAAGTTACTTTCTTTTATTATGGCATCCTCTTTTACTTGGGTTAGGGATGCAGGTTCTCTATTGACTGCCGGGGCAAACACTTTTGGGCCAGAATTTGAAAATTCTGTGAGCATGTTTGAGATGGCTCAATTTGCTCTTGAAGTACTTGATGGTGGCTTATATTCATTAAAGACACTTGGTGAAGAAAGTGGGTTGAGTTCAGTTATTTTAGGTGCAATTTTTCTGATTGACTGGGAGTTTCTGGTATTACTGACAACGATAGATGATGCACCTGATGATAAATCAAGGGAAAAATTGAAGGCCAGGCTGGGATTTGGTGAATCTTTTCATGCATTTAGATGTAAGATAAGTAATCAATTCTGGAAAACACTCAGCTTACACAATCGACAGGCACTTGGAAAAAATTTGATTCAGTGTATGAGATCCGCCATCTTCAATGAAGAGGAATTAGACACAGAGAAGTTCACATCTCTGTGCTGCTTGTGGATGCTTGAAATTCTGGACTGTCTCTCTCAGGATCCCTATGAGGAACAAAATCTGCTGGACCAACTTCTCTGCCGAGGTGATAGATGGCCTTTGTGGATAGTTCCGGATTTTAGCAGGCCAGAGGGAATAGTTGCCAAGGACTTCTCTATCCAAGACTTTGGCCACCGCAAGTTTATTTCATTTATTGACAAGATGATATCAGAAATTGGGATTGAAAGAGTTGTTGGGGGTTATGCTAGATATACTCTCCCTCCCTCTGTGGAAGCAACAAATGAAAAGCCGACTCGATCCTGGCTTGCTGCTGAAATATTATGCTCGTGGAAATGGCCAGGAGGAAGTGCTGTAGCTTCATTCTTACCTTCATTGAGTGCCTTTGCAAAGAGTAAAAATTTCTCTTCCCAGGAGAGCTTGCTAGATTTTATTTTCAACATTCTACTTGATGGCACACTTGTTCAGGGAGGATGTGCTGCACAGAATTTTGTCTATCTGTGTCCTACTTCAAGTGATGAAGTGGAGGATATTGAAGAACCATTCTTAAGAGCTCTTGTAGCTTTTCTTTTAACTTTGTTTAATGATAAAATATGGGGTACTGAGAAAGCTATGGAGCTGTTTGCATTGCTTGTAAATAAACTTTATGTTGGAGAAGCAACAAATGCAAACTGTTTGAGGATTCTTCCTCTGATTGTTAATGTTCTTATTCAACCATTAAGTCAAAGAAGCATCAGATGTAATGACTCTAGCGGAGACGCTCAACATGATTCTTCTGGGGAAAATCATGTGCAAGATGTTATTGAAGGCTGGCTAAAAAAAGCTCTATCATTTCCGCCTTTGATCACGTGGTGGCAAACAGGAGAAGATATGGAAGATTGGATGCAGCTGGTTATATCTTGTTATCCCATTAGTGCAGTAGAGGACATACAAACACCGAAGCTGGAGAGAAAGATCAGCTCCGTAGAGAGAAAGCTCCTAATTGAATTATTCCGGAAGCAGAGACATGGTGTTGGCACACCAGCTGTAATTAATCAGCTTCCAGTTGTGCAGATGTTGCTATCAAAGCTTATGGTCGTTTCAGTTGGTTATTGTTGGAAAGAATTTGATGAAGAAGATTGGGAGTTTGTGTTATCTCAGATAAGACGCTGGCTGCAGTCTGTTGTTGTAATGATGGAGGAAATTGCTGAAAATGTCAACGACACTATTACTAGCAGCTTGACTTCTGATAACTTGGATGATGTCGTTGATAACCTTGGCAAAATTGTTTTCATTTCAGATCCCTTTCCTATAGACATTGCCAAAAATGCCCTTTTATCATTTTCTCTGTCCTGTGGACATTTCGGTCGCCAACAAGCAGAAGATGCAGATAACTTAAATCCCGTGAGAACAGAAAGATGGGACCCCATCAAAAATAGAATTTTAGAAGGCATCCTTCGATTGTTGTTCTGCACGGGTGTTGCTGAGGCCATTGCAAGCTCTTGTTGTCATGAGGCTGCATTTTTTGTATCAGCGTCGCGATTTGAACATTCCTATTTCTGGGAATTGGTGGCCTCAAATGTGGTCAACTCATCAACAGATGCCATAGATAGAGCAGTGAAGTCAGTTGAATTCTGGGGGCTGAGCAAAGGACCCATTAGCTCTTTGTATGCAATCCTTTTTTCTGCCAATACAGTTCCTCTGTTGCAGTACGCTGCTTACTTTATTCTTTCAACTGAACTTGTTTTACGCCTGGCTATTGTTGAAGAAGACAAAAGTTACTTGGATGGCGTTAGTAACAATGAAGAGGTTTCATCCTCTCTTGACATGTCGACAGAAACAGATATTCATTTGAGAGCTGAAATATCTTGCATGATTGAAAAGTTACCTAGTAATGTTCTTGAGATGGATTTAGTGGCAGATCAAAGGGTGCATGTTTTTCTTGCGTGGTCCTTGTTGTTATCTCATTTAGGGTCATTGCCATCATCATCACCTGCAAGGGAGAGACTAGTCCAGTATGTACAAGATTCTGCTAATCCAGTAATATTAGATTGCCTCTTCCAGCATATTCCTCTAGAACTGTGGATTCTTAAGAAGAAAGACGAAGAGCTTCCTGCTGGCATAGCAGAAGCTGCAGCTGCAGCAACACATTCCATTAGAACTGGTTCCTTATTGTTTGCTGTACAGTCTCTCTGGCCTGTTGAACCACTGAAAATGGCTTCGCTTGCTGGAGCAATGTTTGGTCTGATGCTCCACATTCTTCCCGCCTATGTAAGACAGTGGTCCAATGATTTACGTGACCGCTCTACATTATCAGGAATTGAATCGTTCACAAGAGCATGGTGCAGCCCACATCTCATCGCTGGCGAATTAAGTCAGATTAAAAAGGATGAAATTGCTGACGAGAATTTTACCATTGCTGTAAGCAAATCAGCAAATGAGGTTGTTGCTACTTATACAAAGGATGAGACTGCAATGAATCTAGTAATCCGTCTTCCTTCATCTTACCCATTACGGCCTGTTGATGTTGATTGTACAAGGAGTCTAGGTATTAGTGAAGCGAAGCAGAGGAAATGGTCAATGTCTATGACATCATTCGTACGGAATCAGAATGGAGCTTTAGCAGAAGCTATCCGGATATGGAAGCGCAACTTTGACAAGGAGTTTGAAGGTGTCGAGGAGTGTCCTATTTGTTACAGTGTCATCCATACCGTCAACCACGCCCTTCCACGTCTAGCGTGCAAGACCTGCAAGCACAAGTTCCATTCGGCCTGCCTCTATAAGTGGTTCTCAACTTCTCACAAATCAACTTGCCCATTGTGCCAGTCTCCATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

1904

Amino Acids

212.29

Weight (kDa)

5.5

Isoelectric Point (pI)

47.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ltn1_1st PF22958 60 - 386 5e-90 E3 ubiquitin-protein ligase listerin, N-terminal domain
LTN1_E3_ligase_6th PF22999 1510 - 1753 5.6e-28 LTN1 family HEAT repeat region
UBC_like PF23009 1763 - 1846 8.7e-30 Ubiquitin conjugating domain-like
zf-RING_2 PF13639 1852 - 1900 7.6e-07 Ring finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G58410 AT5G58410
fragaria_vesca FvH4_3g39950 FvH4_3g39950 FvH4_3g39950
malus_domestica MD03G1058100.v1.1
prunus_persica Prupe.6G045900_v2.0.a1 Prupe.6G045900_v2.0.a1 Prupe.6G045900_v2.0.a1
pyrus_communis pycom03g04640
rosa_chinensis RchiOBHm_Chr5g0071961
rosa_laevigata RLG00000036266
rosa_multiflora Rmu_sc0012806.1_g000004
rosa_roxburghii Rroxscaffold_1G00009240
rosa_rugosa Rorug05G0415700
rosa_samantha Rh5AG471700 Rh5BG491700 Rh5CG515500 Rh5DG503100
rosa_wichuraiana Rw5G043850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 899
AarI CACCTGC 2 cut(s) 649, 4876
AasI GACNNNNNNGTC 1 cut(s) 4239
Acc36I ACCTGC 4 cut(s) 649, 2462, 4876, 5630
AccB1I GGYRCC 1 cut(s) 734
AccBSI CCGCTC 1 cut(s) 5178
AccI GTMKAC 1 cut(s) 4706
AccII CGCG 1 cut(s) 4414
AccIII TCCGGA 3 cut(s) 3010, 3888, 5508
AcoI YGGCCR 4 cut(s) 297, 687, 3067, 3233
AcuI CTGAAG 1 cut(s) 495
AcvI CACGTG 1 cut(s) 3744
AcyI GRCGYC 1 cut(s) 65
AdeI CACNNNGTG 3 cut(s) 1162, 2600, 3747
AfiI CCNNNNNNNGG 7 cut(s) 146, 311, 402, 1222, 1925, 2306, 4434
AflII CTTAAG 2 cut(s) 3442, 4964
AflIII ACRYGT 1 cut(s) 4701
AhlI ACTAGT 1 cut(s) 4882
AjiI CACGTC 1 cut(s) 5606
AjnI CCWGG 6 cut(s) 2103, 2723, 3197, 3235, 3306, 4626
AjuI GAANNNNNNNTTGG 2 cut(s) 3114, 3146
AloI GAACNNNNNNTCC 4 cut(s) 1965, 1966, 1997, 1998
Alw21I GWGCWC 2 cut(s) 2023, 3451
AlwNI CAGNNNCTG 3 cut(s) 473, 1844, 5006
Aor13HI TCCGGA 3 cut(s) 3010, 3888, 5508
ArsI GACNNNNNNTTYG 2 cut(s) 5522, 5554
AseI ATTAAT 1 cut(s) 3927
Asp700I GAANNNNTTC 4 cut(s) 1290, 2745, 4983, 5038
AspLEI GCGC 2 cut(s) 418, 5523
AsuC2I CCSGG 1 cut(s) 2491
AsuHPI GGTGA 8 cut(s) 385, 673, 1806, 1940, 2612, 2750, 2996, 4857
AvaII GGWCC 8 cut(s) 440, 1769, 1859, 2965, 4297, 4526, 4824, 5157
BalI TGGCCA 3 cut(s) 689, 3069, 3235
BamHI GGATCC 2 cut(s) 979, 2938
BanI GGYRCC 1 cut(s) 734
BanII GRGCYC 1 cut(s) 3451
BarI GAAGNNNNNNTAC 2 cut(s) 5352, 5384
BauI CACGAG 1 cut(s) 3223
BbrPI CACGTG 1 cut(s) 3744
BbsI GAAGAC 3 cut(s) 1499, 4650, 5360
Bbv12I GWGCWC 2 cut(s) 2023, 3451
BbvCI CCTCAGC 1 cut(s) 4359
BceAI ACGGC 4 cut(s) 61, 383, 573, 5405
BcgI CGANNNNNNTGC 2 cut(s) 1721, 1755
BciT130I CCWGG 6 cut(s) 2105, 2725, 3199, 3237, 3308, 4628
BclI TGATCA 1 cut(s) 3738
BcnI CCSGG 1 cut(s) 2491
BcuI ACTAGT 1 cut(s) 4882
BfmI CTRYAG 6 cut(s) 1614, 3249, 4049, 4185, 5007, 5013
BfoI RGCGCY 1 cut(s) 419
BfrI CTTAAG 2 cut(s) 3442, 4964
BfuAI ACCTGC 4 cut(s) 649, 2462, 4876, 5630
BlpI GCTNAGC 1 cut(s) 4517
BmcAI AGTACT 1 cut(s) 2565
Bme1390I CCNGG 7 cut(s) 2105, 2491, 2725, 3199, 3237, 3308, 4628
Bme18I GGWCC 8 cut(s) 440, 1769, 1859, 2965, 4297, 4526, 4824, 5157
BmgBI CACGTC 1 cut(s) 5606
BmiI GGNNCC 9 cut(s) 441, 736, 981, 1771, 2940, 4298, 4299, 4528, 5041
BmrFI CCNGG 7 cut(s) 2105, 2491, 2725, 3199, 3237, 3308, 4628
BmrI ACTGGG 2 cut(s) 461, 2662
BmuI ACTGGG 2 cut(s) 461, 2662
BoxI GACNNNNGTC 2 cut(s) 1052, 5155
BpiI GAAGAC 3 cut(s) 1499, 4650, 5360
BpmI CTGGAG 5 cut(s) 507, 1607, 2126, 3860, 5124
Bpu10I CCTNAGC 1 cut(s) 4359
Bpu1102I GCTNAGC 1 cut(s) 4517
BpuEI CTTGAG 5 cut(s) 468, 897, 1300, 1405, 4796
BpuMI CCSGG 1 cut(s) 2491
BsaAI YACGTR 3 cut(s) 1518, 3744, 5171
BsaBI GATNNNNATC 1 cut(s) 2153
BsaHI GRCGYC 1 cut(s) 65
BsaJI CCNNGG 7 cut(s) 443, 1985, 2490, 2980, 3042, 3306, 4150
BsaWI WCCGGW 4 cut(s) 2145, 3010, 3888, 5508
BsaXI ACNNNNNCTCC 6 cut(s) 3142, 3172, 4010, 4040, 5407, 5437
Bsc4I CCNNNNNNNGG 7 cut(s) 146, 311, 402, 1222, 1925, 2306, 4434
Bse1I ACTGG 7 cut(s) 467, 2657, 3938, 4888, 4913, 5041, 5698
Bse8I GATNNNNATC 1 cut(s) 2153
BseAI TCCGGA 3 cut(s) 3010, 3888, 5508
BseBI CCWGG 6 cut(s) 2105, 2725, 3199, 3237, 3308, 4628
BseDI CCNNGG 7 cut(s) 443, 1985, 2490, 2980, 3042, 3306, 4150
BseJI GATNNNNATC 1 cut(s) 2153
BseLI CCNNNNNNNGG 7 cut(s) 146, 311, 402, 1222, 1925, 2306, 4434
BseMII CTCAG 9 cut(s) 581, 1617, 2058, 2806, 2948, 3489, 4046, 4350, 4508
BseNI ACTGG 7 cut(s) 467, 2657, 3938, 4888, 4913, 5041, 5698
BseRI GAGGAG 5 cut(s) 129, 1445, 2289, 2292, 5567
BseYI CCCAGC 1 cut(s) 2728
BsgI GTGCAG 7 cut(s) 936, 2106, 3360, 3831, 3965, 4330, 5239
Bsh1236I CGCG 1 cut(s) 4414
Bsh1285I CGRYCG 1 cut(s) 4243
BshNI GGYRCC 1 cut(s) 734
BsiEI CGRYCG 1 cut(s) 4243
BsiHKAI GWGCWC 2 cut(s) 2023, 3451
BsiSI CCGG 6 cut(s) 147, 2146, 2490, 3011, 3889, 5509
BsiWI CGTACG 1 cut(s) 5473
BslFI GGGAC 3 cut(s) 453, 893, 4310
BslI CCNNNNNNNGG 7 cut(s) 146, 311, 402, 1222, 1925, 2306, 4434
BsmBI CGTCTC 2 cut(s) 1905, 3645
BsmFI GGGAC 3 cut(s) 453, 893, 4310
BsmI GAATGC 3 cut(s) 1240, 1292, 2110
Bsp1286I GDGCHC 2 cut(s) 2023, 3451
Bsp13I TCCGGA 3 cut(s) 3010, 3888, 5508
Bsp1407I TGTACA 4 cut(s) 1060, 4894, 5056, 5408
Bsp1720I GCTNAGC 1 cut(s) 4517
Bsp19I CCATGG 2 cut(s) 443, 1985
Bsp68I TCGCGA 1 cut(s) 4414
BspCNI CTCAG 9 cut(s) 582, 1616, 2059, 2805, 2947, 3490, 4045, 4351, 4509
BspEI TCCGGA 3 cut(s) 3010, 3888, 5508
BspFNI CGCG 1 cut(s) 4414
BspHI TCATGA 3 cut(s) 370, 2254, 4384
BspLI GGNNCC 9 cut(s) 441, 736, 981, 1771, 2940, 4298, 4299, 4528, 5041
BspMAI CTGCAG 4 cut(s) 1618, 4053, 5011, 5017
BspMI ACCTGC 4 cut(s) 649, 2462, 4876, 5630
BspQI GCTCTTC 1 cut(s) 4974
BspT107I GGYRCC 1 cut(s) 734
BspTI CTTAAG 2 cut(s) 3442, 4964
BsrBI CCGCTC 1 cut(s) 5178
BsrGI TGTACA 4 cut(s) 1060, 4894, 5056, 5408
BsrI ACTGG 7 cut(s) 467, 2657, 3938, 4888, 4913, 5041, 5698
BssECI CCNNGG 7 cut(s) 443, 1985, 2490, 2980, 3042, 3306, 4150
BssNI GRCGYC 1 cut(s) 65
BssSI CACGAG 1 cut(s) 3223
BssT1I CCWWGG 4 cut(s) 443, 1985, 3042, 4150
Bst2BI CACGAG 1 cut(s) 3223
Bst2UI CCWGG 6 cut(s) 2105, 2725, 3199, 3237, 3308, 4628
Bst6I CTCTTC 7 cut(s) 197, 2403, 2856, 3307, 4674, 4937, 4974
BstACI GRCGYC 1 cut(s) 65
BstAFI CTTAAG 2 cut(s) 3442, 4964
BstAPI GCANNNNNTGC 8 cut(s) 649, 1181, 1597, 3560, 3952, 4259, 5006, 5622
BstAUI TGTACA 4 cut(s) 1060, 4894, 5056, 5408
BstBAI YACGTR 3 cut(s) 1518, 3744, 5171
BstDSI CCRYGG 2 cut(s) 443, 1985
BstFNI CGCG 1 cut(s) 4414
BstH2I RGCGCY 1 cut(s) 419
BstHHI GCGC 2 cut(s) 418, 5523
BstMCI CGRYCG 1 cut(s) 4243
BstNI CCWGG 6 cut(s) 2105, 2725, 3199, 3237, 3308, 4628
BstNSI RCATGY 6 cut(s) 767, 1655, 1727, 2536, 4705, 4811
BstPAI GACNNNNGTC 2 cut(s) 1052, 5155
BstSCI CCNGG 7 cut(s) 2103, 2489, 2723, 3197, 3235, 3306, 4626
BstSFI CTRYAG 6 cut(s) 1614, 3249, 4049, 4185, 5007, 5013
BstUI CGCG 1 cut(s) 4414
BstV2I GAAGAC 3 cut(s) 1499, 4650, 5360
BstX2I RGATCY 7 cut(s) 220, 979, 1115, 2117, 2843, 2938, 4174
BstXI CCANNNNNNTGG 4 cut(s) 1044, 2104, 2247, 3065
BstYI RGATCY 7 cut(s) 220, 979, 1115, 2117, 2843, 2938, 4174
BtgI CCRYGG 2 cut(s) 443, 1985
BtgZI GCGATG 3 cut(s) 165, 1221, 5218
BtrI CACGTC 1 cut(s) 5606
BtsI GCAGTG 4 cut(s) 813, 1845, 2078, 4497
BtuMI TCGCGA 1 cut(s) 4414
BveI ACCTGC 4 cut(s) 649, 2462, 4876, 5630
CaiI CAGNNNCTG 3 cut(s) 473, 1844, 5006
CciI TCATGA 3 cut(s) 370, 2254, 4384
CfoI GCGC 2 cut(s) 418, 5523
CseI GACGC 4 cut(s) 54, 3662, 4050, 4398
CspCI CAANNNNNGTGG 2 cut(s) 679, 714
DraIII CACNNNGTG 3 cut(s) 1162, 2600, 3747
DrdI GACNNNNNNGTC 1 cut(s) 4239
DseDI GACNNNNNNGTC 1 cut(s) 4239
EaeI YGGCCR 4 cut(s) 297, 687, 3067, 3233
Eam1104I CTCTTC 7 cut(s) 197, 2403, 2856, 3307, 4674, 4937, 4974
EarI CTCTTC 7 cut(s) 197, 2403, 2856, 3307, 4674, 4937, 4974
EciI GGCGGA 2 cut(s) 2836, 3720
Ecl136II GAGCTC 1 cut(s) 3449
Eco130I CCWWGG 4 cut(s) 443, 1985, 3042, 4150
Eco24I GRGCYC 1 cut(s) 3451
Eco32I GATATC 2 cut(s) 666, 3105
Eco47I GGWCC 8 cut(s) 440, 1769, 1859, 2965, 4297, 4526, 4824, 5157
Eco53kI GAGCTC 1 cut(s) 3449
Eco57I CTGAAG 1 cut(s) 495
Eco72I CACGTG 1 cut(s) 3744
EcoICRI GAGCTC 1 cut(s) 3449
EcoO109I RGGNCCY 3 cut(s) 162, 4297, 4526
EcoRI GAATTC 2 cut(s) 1202, 4505
EcoRII CCWGG 6 cut(s) 2103, 2723, 3197, 3235, 3306, 4626
EcoRV GATATC 2 cut(s) 666, 3105
EcoT14I CCWWGG 4 cut(s) 443, 1985, 3042, 4150
EcoT22I ATGCAT 2 cut(s) 654, 2755
EcoT38I GRGCYC 1 cut(s) 3451
ErhI CCWWGG 4 cut(s) 443, 1985, 3042, 4150
Esp3I CGTCTC 2 cut(s) 1905, 3645
FalI AAGNNNNNCTT 6 cut(s) 1033, 1065, 4314, 4346, 5510, 5542
FaqI GGGAC 3 cut(s) 453, 893, 4310
FauI CCCGC 4 cut(s) 47, 91, 1216, 5146
FbaI TGATCA 1 cut(s) 3738
FblI GTMKAC 1 cut(s) 4706
FriOI GRGCYC 1 cut(s) 3451
GlaI GCGC 2 cut(s) 417, 5522
GsaI CCCAGC 1 cut(s) 2732
GsuI CTGGAG 5 cut(s) 507, 1607, 2126, 3860, 5124
HaeII RGCGCY 1 cut(s) 419
HapII CCGG 6 cut(s) 147, 2146, 2490, 3011, 3889, 5509
HgaI GACGC 4 cut(s) 54, 3662, 4050, 4398
HhaI GCGC 2 cut(s) 418, 5523
Hin1I GRCGYC 1 cut(s) 65
Hin6I GCGC 2 cut(s) 416, 5521
HinP1I GCGC 2 cut(s) 416, 5521
HincII GTYRAC 4 cut(s) 4093, 4462, 4707, 5590
HindII GTYRAC 4 cut(s) 4093, 4462, 4707, 5590
HindIII AAGCTT 4 cut(s) 233, 240, 1385, 3962
HpaII CCGG 6 cut(s) 147, 2146, 2490, 3011, 3889, 5509
HphI GGTGA 8 cut(s) 385, 673, 1806, 1940, 2612, 2750, 2996, 4857
Hpy99I CGWCG 5 cut(s) 23, 70, 82, 119, 4414
HpyCH4IV ACGT 7 cut(s) 114, 906, 1517, 1898, 3743, 5170, 5605
HpySE526I ACGT 7 cut(s) 114, 906, 1517, 1898, 3743, 5170, 5605
Hsp92I GRCGYC 1 cut(s) 65
HspAI GCGC 2 cut(s) 416, 5521
KflI GGGWCCC 1 cut(s) 4297
Kpn2I TCCGGA 3 cut(s) 3010, 3888, 5508
Ksp22I TGATCA 1 cut(s) 3738
LguI GCTCTTC 1 cut(s) 4974
MaeII ACGT 7 cut(s) 114, 906, 1517, 1898, 3743, 5170, 5605
MbiI CCGCTC 1 cut(s) 5178
MflI RGATCY 7 cut(s) 220, 979, 1115, 2117, 2843, 2938, 4174
MhlI GDGCHC 2 cut(s) 2023, 3451
MlsI TGGCCA 3 cut(s) 689, 3069, 3235
MluNI TGGCCA 3 cut(s) 689, 3069, 3235
MlyI GAGTC 3 cut(s) 3184, 3635, 5425
MmeI TCCRAC 6 cut(s) 97, 1025, 1509, 1747, 3526, 3971
Mox20I TGGCCA 3 cut(s) 689, 3069, 3235
Mph1103I ATGCAT 2 cut(s) 654, 2755
MroI TCCGGA 3 cut(s) 3010, 3888, 5508
MroXI GAANNNNTTC 4 cut(s) 1290, 2745, 4983, 5038
MscI TGGCCA 3 cut(s) 689, 3069, 3235
MslI CAYNNNNRTG 4 cut(s) 1000, 2228, 2758, 2893
Msp20I TGGCCA 3 cut(s) 689, 3069, 3235
MspA1I CMGCKG 4 cut(s) 424, 3784, 3920, 5012
MspCI CTTAAG 2 cut(s) 3442, 4964
MspI CCGG 6 cut(s) 147, 2146, 2490, 3011, 3889, 5509
MspR9I CCNGG 7 cut(s) 2105, 2491, 2725, 3199, 3237, 3308, 4628
Mva1269I GAATGC 3 cut(s) 1240, 1292, 2110
MvaI CCWGG 6 cut(s) 2105, 2725, 3199, 3237, 3308, 4628
MvnI CGCG 1 cut(s) 4414
NciI CCSGG 1 cut(s) 2491
NcoI CCATGG 2 cut(s) 443, 1985
NlaIV GGNNCC 9 cut(s) 441, 736, 981, 1771, 2940, 4298, 4299, 4528, 5041
NmeAIII GCCGAG 1 cut(s) 3005
NmuCI GTSAC 2 cut(s) 391, 5171
NruI TCGCGA 1 cut(s) 4414
NsiI ATGCAT 2 cut(s) 654, 2755
NspI RCATGY 6 cut(s) 767, 1655, 1727, 2536, 4705, 4811
PaeI GCATGC 1 cut(s) 1655
PagI TCATGA 3 cut(s) 370, 2254, 4384
PaqCI CACCTGC 2 cut(s) 649, 4876
PciI ACATGT 1 cut(s) 4701
PciSI GCTCTTC 1 cut(s) 4974
PcsI WCGNNNNNNNCGW 2 cut(s) 74, 111
PctI GAATGC 3 cut(s) 1240, 1292, 2110
PdmI GAANNNNTTC 4 cut(s) 1290, 2745, 4983, 5038
Pfl23II CGTACG 1 cut(s) 5473
PflFI GACNNNGTC 1 cut(s) 467
PleI GAGTC 3 cut(s) 3184, 3635, 5424
PmaCI CACGTG 1 cut(s) 3744
PmlI CACGTG 1 cut(s) 3744
PpsI GAGTC 3 cut(s) 3184, 3635, 5424
Ppu21I YACGTR 3 cut(s) 1518, 3744, 5171
PpuMI RGGWCCY 2 cut(s) 4297, 4526
PscI ACATGT 1 cut(s) 4701
PshAI GACNNNNGTC 2 cut(s) 1052, 5155
PshBI ATTAAT 1 cut(s) 3927
PsiI TTATAA 1 cut(s) 899
Psp124BI GAGCTC 1 cut(s) 3451
Psp5II RGGWCCY 2 cut(s) 4297, 4526
Psp6I CCWGG 6 cut(s) 2103, 2723, 3197, 3235, 3306, 4626
PspCI CACGTG 1 cut(s) 3744
PspFI CCCAGC 1 cut(s) 2728
PspGI CCWGG 6 cut(s) 2103, 2723, 3197, 3235, 3306, 4626
PspLI CGTACG 1 cut(s) 5473
PspN4I GGNNCC 9 cut(s) 441, 736, 981, 1771, 2940, 4298, 4299, 4528, 5041
PspPPI RGGWCCY 2 cut(s) 4297, 4526
PsrI GAACNNNNNNTAC 2 cut(s) 4605, 4637
PstI CTGCAG 4 cut(s) 1618, 4053, 5011, 5017
PstNI CAGNNNCTG 3 cut(s) 473, 1844, 5006
PsuI RGATCY 7 cut(s) 220, 979, 1115, 2117, 2843, 2938, 4174
PsyI GACNNNGTC 1 cut(s) 467
PvuII CAGCTG 4 cut(s) 424, 3784, 3920, 5012
RruI TCGCGA 1 cut(s) 4414
RseI CAYNNNNRTG 4 cut(s) 1000, 2228, 2758, 2893
SacI GAGCTC 1 cut(s) 3451
SalI GTCGAC 1 cut(s) 4705
SapI GCTCTTC 1 cut(s) 4974
ScaI AGTACT 1 cut(s) 2565
SchI GAGTC 3 cut(s) 3184, 3635, 5425
ScrFI CCNGG 7 cut(s) 2105, 2491, 2725, 3199, 3237, 3308, 4628
SduI GDGCHC 2 cut(s) 2023, 3451
SfcI CTRYAG 6 cut(s) 1614, 3249, 4049, 4185, 5007, 5013
SinI GGWCC 8 cut(s) 440, 1769, 1859, 2965, 4297, 4526, 4824, 5157
SmiMI CAYNNNNRTG 4 cut(s) 1000, 2228, 2758, 2893
SmlI CTYRAG 7 cut(s) 483, 912, 1315, 1420, 3442, 4775, 4964
SmoI CTYRAG 7 cut(s) 483, 912, 1315, 1420, 3442, 4775, 4964
SpeI ACTAGT 1 cut(s) 4882
SphI GCATGC 1 cut(s) 1655
SspI AATATT 4 cut(s) 827, 2060, 3216, 4920
SstI GAGCTC 1 cut(s) 3451
StyD4I CCNGG 7 cut(s) 2103, 2489, 2723, 3197, 3235, 3306, 4626
StyI CCWWGG 4 cut(s) 443, 1985, 3042, 4150
TaiI ACGT 7 cut(s) 117, 909, 1520, 1901, 3746, 5173, 5608
TaqII GACCGA 1 cut(s) 4229
TatI WGTACW 7 cut(s) 1060, 1072, 2333, 2563, 4894, 5056, 5408
TauI GCSGC 5 cut(s) 65, 95, 98, 299, 361
TseFI GTSAC 2 cut(s) 391, 5171
Tsp45I GTSAC 2 cut(s) 391, 5171
TspGWI ACGGA 7 cut(s) 36, 299, 854, 2202, 3847, 5354, 5491
Tth111I GACNNNGTC 1 cut(s) 467
Vha464I CTTAAG 2 cut(s) 3442, 4964
VpaK11BI GGWCC 8 cut(s) 440, 1769, 1859, 2965, 4297, 4526, 4824, 5157
VspI ATTAAT 1 cut(s) 3927
XbaI TCTAGA 2 cut(s) 2263, 4948
XceI RCATGY 6 cut(s) 767, 1655, 1727, 2536, 4705, 4811
XmiI GTMKAC 1 cut(s) 4706
XmnI GAANNNNTTC 4 cut(s) 1290, 2745, 4983, 5038
ZrmI AGTACT 1 cut(s) 2565
Zsp2I ATGCAT 2 cut(s) 654, 2755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.