FvH4_3g40280

EPIDERMAL PATTERNING FACTOR-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0010795
Physical Location & Seq
Reverse (-)
34029807 .. 34031860
2054 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g40280.t2

Sequence Viewer

Length: 408 bp
ATGGAGTGGAAGAGCAAGAGAGTACTAAGCAGCAGCTGGTTTATGATCAACAAGCTCACCTCCCTTTTCTTCTTGCTCTCAATCTCTGCACTACTATTCACCATCATCAACTCTACTTCTACATGTACTAAAGATTGTATCTTTCCCTCAAGTAGTGCTCAAGCTGATACAAATTTTCAGGAGGAGCAGAGGGAGGAGAGTGTGATGATGATGATGACGCCTTTGAGCTTGGCGAGGAGATACTTGAGCGGGCCAGGGTCGTCGCCGCCTCGCTGCAACTCCAAGTGCGGCAGGTGCACGCCGTGCAAGCCGGTTCATGTGCCGGTGCCGCCTGGAACTCCGGTGACGGCGGAGTACTACCCGGAAGCGTGGAGGTGCAAGTGTGGCAACAGGTTGTACATGCCATGA

Protein Analysis

136

Amino Acids

15.19

Weight (kDa)

9.0

Isoelectric Point (pI)

54.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPF PF17181 71 - 121 1.3e-20 Epidermal patterning factor proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010795)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G30370 AT2G30370 AT2G30370
fragaria_vesca FvH4_3g40280 FvH4_3g40280 FvH4_6g29861
malus_domestica MD11G1055500.v1.1
prunus_persica Prupe.6G043000_v2.0.a1
pyrus_communis pycom03g04280 pycom11g04530
rosa_chinensis RchiOBHm_Chr5g0072621
rosa_laevigata RLG00000036317
rosa_multiflora Rmu_sc0007873.1_g000018
rosa_roxburghii Rroxscaffold_1G00008570 Rroxscaffold_2G00108910
rosa_rugosa Rorug02G0326300 Rorug05G0418900
rosa_samantha Rh5AG475700 Rh5DG508300
rosa_wichuraiana Rw5G044170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 282
Acc36I ACCTGC 1 cut(s) 282
AccB1I GGYRCC 1 cut(s) 325
AccBSI CCGCTC 1 cut(s) 249
AciI CCGC 5 cut(s) 249, 266, 288, 329, 350
AcsI RAATTY 1 cut(s) 172
AcyI GRCGYC 1 cut(s) 218
AdeI CACNNNGTG 1 cut(s) 303
AfaI GTAC 4 cut(s) 24, 127, 356, 398
AflIII ACRYGT 1 cut(s) 122
AjnI CCWGG 2 cut(s) 253, 331
AluBI AGCT 4 cut(s) 36, 55, 164, 228
AluI AGCT 4 cut(s) 36, 55, 164, 228
Alw21I GWGCWC 2 cut(s) 160, 299
Alw44I GTGCAC 1 cut(s) 295
AlwNI CAGNNNCTG 1 cut(s) 36
AoxI GGCC 1 cut(s) 251
ApaLI GTGCAC 1 cut(s) 295
ApeKI GCWGC 3 cut(s) 30, 33, 273
ApoI RAATTY 1 cut(s) 172
AspS9I GGNCC 1 cut(s) 251
AsuC2I CCSGG 1 cut(s) 362
AsuHPI GGTGA 3 cut(s) 49, 91, 355
BaeGI GKGCMC 1 cut(s) 299
BanI GGYRCC 1 cut(s) 325
Bbv12I GWGCWC 2 cut(s) 160, 299
BbvI GCAGC 3 cut(s) 42, 45, 260
BccI CCATC 1 cut(s) 110
BceAI ACGGC 2 cut(s) 286, 363
BciT130I CCWGG 2 cut(s) 255, 333
BclI TGATCA 1 cut(s) 45
BcnI CCSGG 1 cut(s) 362
BfuAI ACCTGC 1 cut(s) 282
BisI GCNGC 6 cut(s) 31, 34, 266, 274, 289, 329
BlsI GCNGC 6 cut(s) 32, 35, 267, 275, 290, 330
BmcAI AGTACT 2 cut(s) 24, 356
Bme1390I CCNGG 3 cut(s) 255, 333, 362
BmgT120I GGNCC 1 cut(s) 251
BmiI GGNNCC 1 cut(s) 327
BmrFI CCNGG 3 cut(s) 255, 333, 362
BpuEI CTTGAG 3 cut(s) 133, 144, 265
BpuMI CCSGG 1 cut(s) 362
BsaHI GRCGYC 1 cut(s) 218
BsaJI CCNNGG 1 cut(s) 254
BsaWI WCCGGW 1 cut(s) 340
Bse118I RCCGGY 2 cut(s) 310, 322
BseBI CCWGG 2 cut(s) 255, 333
BseDI CCNNGG 1 cut(s) 254
BseRI GAGGAG 3 cut(s) 197, 209, 250
BseSI GKGCMC 1 cut(s) 299
BseXI GCAGC 3 cut(s) 42, 45, 260
BsgI GTGCAG 1 cut(s) 72
BshFI GGCC 1 cut(s) 253
BshNI GGYRCC 1 cut(s) 325
BsiHKAI GWGCWC 2 cut(s) 160, 299
BsiSI CCGG 4 cut(s) 311, 323, 341, 362
BsnI GGCC 1 cut(s) 253
Bsp1286I GDGCHC 2 cut(s) 160, 299
Bsp1407I TGTACA 1 cut(s) 396
Bsp143I GATC 1 cut(s) 45
BspACI CCGC 5 cut(s) 249, 266, 288, 329, 350
BspANI GGCC 1 cut(s) 253
BspLI GGNNCC 1 cut(s) 327
BspMI ACCTGC 1 cut(s) 282
BspQI GCTCTTC 1 cut(s) 5
BspT107I GGYRCC 1 cut(s) 325
BsrBI CCGCTC 1 cut(s) 249
BsrFI RCCGGY 2 cut(s) 310, 322
BsrGI TGTACA 1 cut(s) 396
BssAI RCCGGY 2 cut(s) 310, 322
BssECI CCNNGG 1 cut(s) 254
BssMI GATC 1 cut(s) 45
BssNI GRCGYC 1 cut(s) 218
Bst2UI CCWGG 2 cut(s) 255, 333
Bst6I CTCTTC 1 cut(s) 5
BstACI GRCGYC 1 cut(s) 218
BstAPI GCANNNNNTGC 1 cut(s) 303
BstAUI TGTACA 1 cut(s) 396
BstC8I GCNNGC 3 cut(s) 251, 299, 308
BstDEI CTNAG 1 cut(s) 26
BstKTI GATC 1 cut(s) 48
BstMBI GATC 1 cut(s) 45
BstMWI GCNNNNNNNGC 5 cut(s) 294, 303, 307, 328, 384
BstNI CCWGG 2 cut(s) 255, 333
BstNSI RCATGY 2 cut(s) 126, 403
BstSCI CCNGG 3 cut(s) 253, 331, 360
BstSLI GKGCMC 1 cut(s) 299
BstV1I GCAGC 3 cut(s) 42, 45, 260
BsuRI GGCC 1 cut(s) 253
BveI ACCTGC 1 cut(s) 282
Cac8I GCNNGC 3 cut(s) 251, 299, 308
CaiI CAGNNNCTG 1 cut(s) 36
Cfr10I RCCGGY 2 cut(s) 310, 322
Cfr13I GGNCC 1 cut(s) 251
CseI GACGC 1 cut(s) 226
Csp6I GTAC 4 cut(s) 23, 126, 355, 397
CviAII CATG 4 cut(s) 123, 317, 400, 405
CviJI RGCY 6 cut(s) 36, 55, 164, 228, 253, 310
CviKI_1 RGCY 6 cut(s) 36, 55, 164, 228, 253, 310
CviQI GTAC 4 cut(s) 23, 126, 355, 397
DdeI CTNAG 1 cut(s) 26
DpnI GATC 1 cut(s) 47
DpnII GATC 1 cut(s) 45
DraIII CACNNNGTG 1 cut(s) 303
Eam1104I CTCTTC 1 cut(s) 5
EarI CTCTTC 1 cut(s) 5
EciI GGCGGA 1 cut(s) 365
EcoRII CCWGG 2 cut(s) 253, 331
FaeI CATG 4 cut(s) 126, 320, 403, 408
FaiI YATR 5 cut(s) 44, 124, 318, 401, 406
FatI CATG 4 cut(s) 122, 316, 399, 404
FauI CCCGC 1 cut(s) 242
FbaI TGATCA 1 cut(s) 45
Fnu4HI GCNGC 6 cut(s) 31, 34, 266, 274, 289, 329
Fsp4HI GCNGC 6 cut(s) 31, 34, 266, 274, 289, 329
GluI GCNGC 6 cut(s) 31, 34, 266, 274, 289, 329
HaeIII GGCC 1 cut(s) 253
HapII CCGG 4 cut(s) 311, 323, 341, 362
HgaI GACGC 1 cut(s) 226
Hin1I GRCGYC 1 cut(s) 218
Hin1II CATG 4 cut(s) 126, 320, 403, 408
HpaII CCGG 4 cut(s) 311, 323, 341, 362
HphI GGTGA 3 cut(s) 49, 91, 355
Hpy166II GTNNAC 1 cut(s) 297
Hpy188III TCNNGA 1 cut(s) 179
Hpy8I GTNNAC 1 cut(s) 297
Hpy99I CGWCG 1 cut(s) 265
HpyCH4V TGCA 5 cut(s) 89, 276, 297, 306, 378
HpyF10VI GCNNNNNNNGC 5 cut(s) 294, 303, 307, 328, 384
HpyF3I CTNAG 1 cut(s) 26
Hsp92I GRCGYC 1 cut(s) 218
Hsp92II CATG 4 cut(s) 126, 320, 403, 408
Ksp22I TGATCA 1 cut(s) 45
Kzo9I GATC 1 cut(s) 45
LguI GCTCTTC 1 cut(s) 5
LmnI GCTCC 1 cut(s) 184
Lsp1109I GCAGC 3 cut(s) 42, 45, 260
MaeIII GTNAC 1 cut(s) 343
MalI GATC 1 cut(s) 47
MbiI CCGCTC 1 cut(s) 249
MboI GATC 1 cut(s) 45
MboII GAAGA 2 cut(s) 22, 61
MhlI GDGCHC 2 cut(s) 160, 299
MluCI AATT 1 cut(s) 172
MnlI CCTC 8 cut(s) 70, 157, 175, 183, 187, 228, 279, 366
MspA1I CMGCKG 1 cut(s) 36
MspI CCGG 4 cut(s) 311, 323, 341, 362
MspR9I CCNGG 3 cut(s) 255, 333, 362
MvaI CCWGG 2 cut(s) 255, 333
MwoI GCNNNNNNNGC 5 cut(s) 294, 303, 307, 328, 384
NciI CCSGG 1 cut(s) 362
NdeII GATC 1 cut(s) 45
NlaIII CATG 4 cut(s) 126, 320, 403, 408
NlaIV GGNNCC 1 cut(s) 327
NmuCI GTSAC 1 cut(s) 343
NspI RCATGY 2 cut(s) 126, 403
PaqCI CACCTGC 1 cut(s) 282
PciI ACATGT 1 cut(s) 122
PciSI GCTCTTC 1 cut(s) 5
PkrI GCNGC 6 cut(s) 32, 35, 267, 275, 290, 330
PscI ACATGT 1 cut(s) 122
Psp6I CCWGG 2 cut(s) 253, 331
PspGI CCWGG 2 cut(s) 253, 331
PspN4I GGNNCC 1 cut(s) 327
PspPI GGNCC 1 cut(s) 251
PstNI CAGNNNCTG 1 cut(s) 36
PvuII CAGCTG 1 cut(s) 36
RsaI GTAC 4 cut(s) 24, 127, 356, 398
RsaNI GTAC 4 cut(s) 23, 126, 355, 397
SapI GCTCTTC 1 cut(s) 5
SatI GCNGC 6 cut(s) 31, 34, 266, 274, 289, 329
Sau3AI GATC 1 cut(s) 45
Sau96I GGNCC 1 cut(s) 251
ScaI AGTACT 2 cut(s) 24, 356
ScrFI CCNGG 3 cut(s) 255, 333, 362
SduI GDGCHC 2 cut(s) 160, 299
SetI ASST 8 cut(s) 38, 57, 62, 166, 230, 296, 377, 395
SmlI CTYRAG 3 cut(s) 148, 159, 244
SmoI CTYRAG 3 cut(s) 148, 159, 244
Sse9I AATT 1 cut(s) 172
SsiI CCGC 5 cut(s) 249, 266, 288, 329, 350
StyD4I CCNGG 3 cut(s) 253, 331, 360
TasI AATT 1 cut(s) 172
TatI WGTACW 4 cut(s) 22, 125, 354, 396
TauI GCSGC 3 cut(s) 268, 291, 331
TseFI GTSAC 1 cut(s) 343
TseI GCWGC 3 cut(s) 30, 33, 273
Tsp45I GTSAC 1 cut(s) 343
TspDTI ATGAA 1 cut(s) 305
VneI GTGCAC 1 cut(s) 295
XapI RAATTY 1 cut(s) 172
XceI RCATGY 2 cut(s) 126, 403
ZrmI AGTACT 2 cut(s) 24, 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.