Rw5G044170

EPIDERMAL PATTERNING FACTOR-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0010795
Physical Location & Seq
Reverse (-)
77741924 .. 77743650
1727 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G044170.1

Sequence Viewer

Length: 396 bp
ATGGAGTGGAAGCGACAGAGAGTATTAAGCAGCTGGTTTATCAACAAGCTCACCTCCCTTTTCTTCTTGCTCTCAATCTCTGCACTGCTATTCACCATCATCACCTCTACATCTACATGTTCTCTAGATTGTAGCTTTCCCTTAAGTACCGCTAAAGCTGACACAAATTTTCAGGAGGAGCAGAGGGGGAAGAAGGCGATGGTGATGCCTTTGAGCTTGGCGAGGAGGTACTTGAGCGGGCCCGGATCGTCGCCGCCTCGCTGCACTTCCAAGTGCGGCAGGTGCACGCCGTGCAAGCCTGTTCACGTGCCCGTGCCGCCCGGAACTCCGGTGACAGCAGAGTACTACCCGGAAGCGTGGAGGTGCAAGTGTGGGAACAAGCTGTACATGCCATGA

Protein Analysis

131

Amino Acids

14.64

Weight (kDa)

9.47

Isoelectric Point (pI)

51.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPF PF17181 81 - 131 1.2e-20 Epidermal patterning factor proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010795)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G30370 AT2G30370 AT2G30370
fragaria_vesca FvH4_3g40280 FvH4_3g40280 FvH4_6g29861
malus_domestica MD11G1055500.v1.1
prunus_persica Prupe.6G043000_v2.0.a1
pyrus_communis pycom03g04280 pycom11g04530
rosa_chinensis RchiOBHm_Chr5g0072621
rosa_laevigata RLG00000036317
rosa_multiflora Rmu_sc0007873.1_g000018
rosa_roxburghii Rroxscaffold_1G00008570 Rroxscaffold_2G00108910
rosa_rugosa Rorug02G0326300 Rorug05G0418900
rosa_samantha Rh5AG475700 Rh5DG508300
rosa_wichuraiana Rw5G044170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 270
Acc36I ACCTGC 1 cut(s) 270
AccBSI CCGCTC 1 cut(s) 237
AciI CCGC 5 cut(s) 150, 237, 254, 276, 317
AclWI GGATC 1 cut(s) 253
AcsI RAATTY 1 cut(s) 166
AcvI CACGTG 1 cut(s) 307
AdeI CACNNNGTG 1 cut(s) 291
AfaI GTAC 4 cut(s) 148, 230, 344, 386
AflII CTTAAG 1 cut(s) 142
AflIII ACRYGT 1 cut(s) 116
AluBI AGCT 6 cut(s) 33, 49, 135, 158, 216, 382
AluI AGCT 6 cut(s) 33, 49, 135, 158, 216, 382
Alw21I GWGCWC 1 cut(s) 287
Alw44I GTGCAC 1 cut(s) 283
AlwI GGATC 1 cut(s) 253
AoxI GGCC 1 cut(s) 239
ApaI GGGCCC 1 cut(s) 243
ApaLI GTGCAC 1 cut(s) 283
ApeKI GCWGC 2 cut(s) 30, 261
ApoI RAATTY 1 cut(s) 166
AspS9I GGNCC 2 cut(s) 239, 240
AsuC2I CCSGG 3 cut(s) 243, 321, 350
AsuHPI GGTGA 5 cut(s) 43, 85, 94, 214, 343
BaeGI GKGCMC 3 cut(s) 243, 287, 312
BanII GRGCYC 1 cut(s) 243
BbrPI CACGTG 1 cut(s) 307
Bbv12I GWGCWC 1 cut(s) 287
BbvI GCAGC 2 cut(s) 42, 248
BccI CCATC 2 cut(s) 104, 193
BceAI ACGGC 1 cut(s) 274
BcgI CGANNNNNNTGC 2 cut(s) 187, 221
BcnI CCSGG 3 cut(s) 243, 321, 350
BfaI CTAG 1 cut(s) 125
BfrI CTTAAG 1 cut(s) 142
BfuAI ACCTGC 1 cut(s) 270
BisI GCNGC 5 cut(s) 31, 254, 262, 277, 317
BlsI GCNGC 5 cut(s) 32, 255, 263, 278, 318
BmcAI AGTACT 1 cut(s) 344
Bme1390I CCNGG 3 cut(s) 243, 321, 350
BmgT120I GGNCC 2 cut(s) 239, 240
BmiI GGNNCC 1 cut(s) 241
BmrFI CCNGG 3 cut(s) 243, 321, 350
BmsI GCATC 1 cut(s) 195
BpuEI CTTGAG 1 cut(s) 253
BpuMI CCSGG 3 cut(s) 243, 321, 350
BsaAI YACGTR 1 cut(s) 307
BsaWI WCCGGW 1 cut(s) 328
BseRI GAGGAG 2 cut(s) 191, 238
BseSI GKGCMC 3 cut(s) 243, 287, 312
BseXI GCAGC 2 cut(s) 42, 248
BsgI GTGCAG 2 cut(s) 66, 247
BshFI GGCC 1 cut(s) 241
BsiHKAI GWGCWC 1 cut(s) 287
BsiSI CCGG 4 cut(s) 243, 321, 329, 350
BsnI GGCC 1 cut(s) 241
Bsp120I GGGCCC 1 cut(s) 239
Bsp1286I GDGCHC 3 cut(s) 243, 287, 312
Bsp1407I TGTACA 1 cut(s) 384
Bsp143I GATC 1 cut(s) 245
BspACI CCGC 5 cut(s) 150, 237, 254, 276, 317
BspANI GGCC 1 cut(s) 241
BspLI GGNNCC 1 cut(s) 241
BspMI ACCTGC 1 cut(s) 270
BspPI GGATC 1 cut(s) 253
BspTI CTTAAG 1 cut(s) 142
BsrBI CCGCTC 1 cut(s) 237
BsrGI TGTACA 1 cut(s) 384
BssMI GATC 1 cut(s) 245
BstAFI CTTAAG 1 cut(s) 142
BstAPI GCANNNNNTGC 1 cut(s) 291
BstAUI TGTACA 1 cut(s) 384
BstBAI YACGTR 1 cut(s) 307
BstC8I GCNNGC 3 cut(s) 239, 287, 296
BstKTI GATC 1 cut(s) 248
BstMBI GATC 1 cut(s) 245
BstMWI GCNNNNNNNGC 5 cut(s) 282, 291, 295, 316, 388
BstNSI RCATGY 2 cut(s) 120, 391
BstSCI CCNGG 3 cut(s) 241, 319, 348
BstSLI GKGCMC 3 cut(s) 243, 287, 312
BstV1I GCAGC 2 cut(s) 42, 248
BsuRI GGCC 1 cut(s) 241
BtgZI GCGATG 1 cut(s) 212
BtsI GCAGTG 1 cut(s) 83
BtsIMutI CAGTG 1 cut(s) 83
BveI ACCTGC 1 cut(s) 270
Cac8I GCNNGC 3 cut(s) 239, 287, 296
Cfr13I GGNCC 2 cut(s) 239, 240
Csp6I GTAC 4 cut(s) 147, 229, 343, 385
CviAII CATG 3 cut(s) 117, 388, 393
CviJI RGCY 8 cut(s) 33, 49, 135, 158, 216, 241, 298, 382
CviKI_1 RGCY 8 cut(s) 33, 49, 135, 158, 216, 241, 298, 382
CviQI GTAC 4 cut(s) 147, 229, 343, 385
DpnI GATC 1 cut(s) 247
DpnII GATC 1 cut(s) 245
DraIII CACNNNGTG 1 cut(s) 291
Eco24I GRGCYC 1 cut(s) 243
Eco72I CACGTG 1 cut(s) 307
EcoT38I GRGCYC 1 cut(s) 243
FaeI CATG 3 cut(s) 120, 391, 396
FaiI YATR 3 cut(s) 118, 389, 394
FatI CATG 3 cut(s) 116, 387, 392
FauI CCCGC 1 cut(s) 230
Fnu4HI GCNGC 5 cut(s) 31, 254, 262, 277, 317
FriOI GRGCYC 1 cut(s) 243
Fsp4HI GCNGC 5 cut(s) 31, 254, 262, 277, 317
FspBI CTAG 1 cut(s) 125
GluI GCNGC 5 cut(s) 31, 254, 262, 277, 317
HaeIII GGCC 1 cut(s) 241
HapII CCGG 4 cut(s) 243, 321, 329, 350
Hin1II CATG 3 cut(s) 120, 391, 396
HpaII CCGG 4 cut(s) 243, 321, 329, 350
HphI GGTGA 5 cut(s) 43, 85, 94, 214, 343
Hpy166II GTNNAC 2 cut(s) 285, 304
Hpy188III TCNNGA 2 cut(s) 125, 173
Hpy8I GTNNAC 2 cut(s) 285, 304
Hpy99I CGWCG 1 cut(s) 253
HpyAV CCTTC 1 cut(s) 187
HpyCH4IV ACGT 1 cut(s) 306
HpyCH4V TGCA 5 cut(s) 83, 264, 285, 294, 366
HpyF10VI GCNNNNNNNGC 5 cut(s) 282, 291, 295, 316, 388
HpySE526I ACGT 1 cut(s) 306
Hsp92II CATG 3 cut(s) 120, 391, 396
Kzo9I GATC 1 cut(s) 245
LmnI GCTCC 1 cut(s) 178
LpnPI CCDG 8 cut(s) 19, 158, 256, 265, 312, 334, 342, 363
Lsp1109I GCAGC 2 cut(s) 42, 248
LweI GCATC 1 cut(s) 195
MaeI CTAG 1 cut(s) 125
MaeII ACGT 1 cut(s) 306
MaeIII GTNAC 1 cut(s) 331
MalI GATC 1 cut(s) 247
MbiI CCGCTC 1 cut(s) 237
MboI GATC 1 cut(s) 245
MboII GAAGA 2 cut(s) 55, 202
MhlI GDGCHC 3 cut(s) 243, 287, 312
MluCI AATT 1 cut(s) 166
MnlI CCTC 8 cut(s) 64, 115, 169, 177, 216, 219, 267, 354
MseI TTAA 2 cut(s) 26, 143
MslI CAYNNNNRTG 1 cut(s) 115
MspA1I CMGCKG 1 cut(s) 33
MspCI CTTAAG 1 cut(s) 142
MspI CCGG 4 cut(s) 243, 321, 329, 350
MspR9I CCNGG 3 cut(s) 243, 321, 350
MwoI GCNNNNNNNGC 5 cut(s) 282, 291, 295, 316, 388
NciI CCSGG 3 cut(s) 243, 321, 350
NdeII GATC 1 cut(s) 245
NlaIII CATG 3 cut(s) 120, 391, 396
NlaIV GGNNCC 1 cut(s) 241
NmuCI GTSAC 1 cut(s) 331
NspI RCATGY 2 cut(s) 120, 391
PaqCI CACCTGC 1 cut(s) 270
PciI ACATGT 1 cut(s) 116
PkrI GCNGC 5 cut(s) 32, 255, 263, 278, 318
PmaCI CACGTG 1 cut(s) 307
PmlI CACGTG 1 cut(s) 307
Ppu21I YACGTR 1 cut(s) 307
PscI ACATGT 1 cut(s) 116
PspCI CACGTG 1 cut(s) 307
PspN4I GGNNCC 1 cut(s) 241
PspOMI GGGCCC 1 cut(s) 239
PspPI GGNCC 2 cut(s) 239, 240
PsrI GAACNNNNNNTAC 1 cut(s) 368
PvuII CAGCTG 1 cut(s) 33
RsaI GTAC 4 cut(s) 148, 230, 344, 386
RsaNI GTAC 4 cut(s) 147, 229, 343, 385
RseI CAYNNNNRTG 1 cut(s) 115
SaqAI TTAA 2 cut(s) 26, 143
SatI GCNGC 5 cut(s) 31, 254, 262, 277, 317
Sau3AI GATC 1 cut(s) 245
Sau96I GGNCC 2 cut(s) 239, 240
ScaI AGTACT 1 cut(s) 344
ScrFI CCNGG 3 cut(s) 243, 321, 350
SduI GDGCHC 3 cut(s) 243, 287, 312
SfaNI GCATC 1 cut(s) 195
SmiMI CAYNNNNRTG 1 cut(s) 115
SmlI CTYRAG 2 cut(s) 142, 232
SmoI CTYRAG 2 cut(s) 142, 232
Sse9I AATT 1 cut(s) 166
SsiI CCGC 5 cut(s) 150, 237, 254, 276, 317
SspMI CTAG 1 cut(s) 125
StyD4I CCNGG 3 cut(s) 241, 319, 348
TaiI ACGT 1 cut(s) 309
TasI AATT 1 cut(s) 166
TatI WGTACW 2 cut(s) 342, 384
TauI GCSGC 3 cut(s) 256, 279, 319
Tru1I TTAA 2 cut(s) 26, 143
Tru9I TTAA 2 cut(s) 26, 143
TscAI CASTG 1 cut(s) 90
TseFI GTSAC 1 cut(s) 331
TseI GCWGC 2 cut(s) 30, 261
Tsp45I GTSAC 1 cut(s) 331
TspRI CASTG 1 cut(s) 90
Vha464I CTTAAG 1 cut(s) 142
VneI GTGCAC 1 cut(s) 283
XapI RAATTY 1 cut(s) 166
XbaI TCTAGA 1 cut(s) 124
XceI RCATGY 2 cut(s) 120, 391
XspI CTAG 1 cut(s) 125
ZrmI AGTACT 1 cut(s) 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.