pycom03g04280

EPIDERMAL PATTERNING FACTOR-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0010795
Physical Location & Seq
Forward (+)
3369172 .. 3370547
1376 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g04280.1

Sequence Viewer

Length: 384 bp
ATGGAGTTGAAGGCACTTCACAGACTAATAAGGTTCACCGCCATCAGCTTTTTCTCAATTTATGCACTTTTCACCACCATCAGCTCCACTACCAGTACATGCCCAGATATTGCGTGTACCTTCACGGCTAAACTCGATTCATATTTTCAGGAATTTGGGAAGCACGGTGACAAAATGAGGGAGCAGAGGATGATGCCCTTGAGCTTGGCTAGGAGGTACCTGAGTGGACCTGGATCGTCGCCGCCTCGGTGCACATCCAAGTGCGGCAGGTGCACGCCGTGCAAGCCAGTTCACGTGTCTGTGCCACCCGGGACGCCGGTGATGGCGGAGTACTACCCGGAGGCTTGGAGGTGTAAATGTGGCAACAAATTGTACATGCCATGA

Protein Analysis

128

Amino Acids

14.3

Weight (kDa)

9.34

Isoelectric Point (pI)

58.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPF PF17181 77 - 127 7.1e-21 Epidermal patterning factor proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010795)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G30370 AT2G30370 AT2G30370
fragaria_vesca FvH4_3g40280 FvH4_3g40280 FvH4_6g29861
malus_domestica MD11G1055500.v1.1
prunus_persica Prupe.6G043000_v2.0.a1
pyrus_communis pycom03g04280 pycom11g04530
rosa_chinensis RchiOBHm_Chr5g0072621
rosa_laevigata RLG00000036317
rosa_multiflora Rmu_sc0007873.1_g000018
rosa_roxburghii Rroxscaffold_1G00008570 Rroxscaffold_2G00108910
rosa_rugosa Rorug02G0326300 Rorug05G0418900
rosa_samantha Rh5AG475700 Rh5DG508300
rosa_wichuraiana Rw5G044170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 258
Acc36I ACCTGC 1 cut(s) 258
Acc65I GGTACC 1 cut(s) 216
AccB1I GGYRCC 1 cut(s) 216
AciI CCGC 4 cut(s) 39, 242, 264, 326
AclWI GGATC 1 cut(s) 241
AcsI RAATTY 1 cut(s) 152
AcvI CACGTG 1 cut(s) 295
AcyI GRCGYC 1 cut(s) 314
AdeI CACNNNGTG 1 cut(s) 279
AfaI GTAC 5 cut(s) 97, 118, 218, 332, 374
AflIII ACRYGT 1 cut(s) 294
AgsI TTSAA 1 cut(s) 10
AjnI CCWGG 1 cut(s) 229
AluBI AGCT 3 cut(s) 48, 84, 204
AluI AGCT 3 cut(s) 48, 84, 204
Alw21I GWGCWC 2 cut(s) 254, 275
Alw44I GTGCAC 2 cut(s) 250, 271
AlwI GGATC 1 cut(s) 241
Ama87I CYCGRG 1 cut(s) 308
ApaLI GTGCAC 2 cut(s) 250, 271
ApoI RAATTY 1 cut(s) 152
Asp718I GGTACC 1 cut(s) 216
AspS9I GGNCC 1 cut(s) 227
AsuC2I CCSGG 3 cut(s) 309, 310, 338
AsuHPI GGTGA 4 cut(s) 28, 64, 179, 331
AvaI CYCGRG 1 cut(s) 308
AvaII GGWCC 1 cut(s) 227
BaeGI GKGCMC 2 cut(s) 254, 275
BaeI ACNNNNGTAYC 2 cut(s) 208, 241
BanI GGYRCC 1 cut(s) 216
BbrPI CACGTG 1 cut(s) 295
Bbv12I GWGCWC 2 cut(s) 254, 275
BccI CCATC 3 cut(s) 50, 86, 316
BceAI ACGGC 2 cut(s) 141, 262
BciT130I CCWGG 1 cut(s) 231
BcnI CCSGG 3 cut(s) 309, 310, 338
BfaI CTAG 1 cut(s) 210
BfuAI ACCTGC 1 cut(s) 258
BisI GCNGC 2 cut(s) 242, 265
BlsI GCNGC 2 cut(s) 243, 266
BmcAI AGTACT 1 cut(s) 332
Bme1390I CCNGG 4 cut(s) 231, 309, 310, 338
Bme18I GGWCC 1 cut(s) 227
BmeT110I CYCGRG 1 cut(s) 308
BmgT120I GGNCC 1 cut(s) 227
BmiI GGNNCC 1 cut(s) 218
BmrFI CCNGG 4 cut(s) 231, 309, 310, 338
BmsI GCATC 1 cut(s) 183
BpuEI CTTGAG 1 cut(s) 220
BpuMI CCSGG 3 cut(s) 309, 310, 338
BsaAI YACGTR 1 cut(s) 295
BsaHI GRCGYC 1 cut(s) 314
BsaJI CCNNGG 2 cut(s) 245, 308
Bse118I RCCGGY 1 cut(s) 316
Bse1I ACTGG 2 cut(s) 93, 287
BseBI CCWGG 1 cut(s) 231
BseDI CCNNGG 2 cut(s) 245, 308
BseGI GGATG 2 cut(s) 195, 254
BseMII CTCAG 1 cut(s) 212
BseNI ACTGG 2 cut(s) 93, 287
BseSI GKGCMC 2 cut(s) 254, 275
BshNI GGYRCC 1 cut(s) 216
BsiHKAI GWGCWC 2 cut(s) 254, 275
BsiHKCI CYCGRG 1 cut(s) 308
BsiSI CCGG 3 cut(s) 309, 317, 338
BslFI GGGAC 1 cut(s) 325
BsmFI GGGAC 1 cut(s) 325
BsoBI CYCGRG 1 cut(s) 308
Bsp1286I GDGCHC 2 cut(s) 254, 275
Bsp1407I TGTACA 1 cut(s) 372
Bsp143I GATC 1 cut(s) 233
BspACI CCGC 4 cut(s) 39, 242, 264, 326
BspCNI CTCAG 1 cut(s) 213
BspLI GGNNCC 1 cut(s) 218
BspMI ACCTGC 1 cut(s) 258
BspPI GGATC 1 cut(s) 241
BspT107I GGYRCC 1 cut(s) 216
BsrFI RCCGGY 1 cut(s) 316
BsrGI TGTACA 1 cut(s) 372
BsrI ACTGG 2 cut(s) 93, 287
BssAI RCCGGY 1 cut(s) 316
BssECI CCNNGG 2 cut(s) 245, 308
BssMI GATC 1 cut(s) 233
BssNI GRCGYC 1 cut(s) 314
Bst2UI CCWGG 1 cut(s) 231
Bst4CI ACNGT 1 cut(s) 167
BstACI GRCGYC 1 cut(s) 314
BstAPI GCANNNNNTGC 1 cut(s) 279
BstAUI TGTACA 1 cut(s) 372
BstBAI YACGTR 1 cut(s) 295
BstC8I GCNNGC 2 cut(s) 275, 284
BstDEI CTNAG 1 cut(s) 221
BstF5I GGATG 2 cut(s) 195, 254
BstKTI GATC 1 cut(s) 236
BstMBI GATC 1 cut(s) 233
BstMWI GCNNNNNNNGC 3 cut(s) 270, 279, 283
BstNI CCWGG 1 cut(s) 231
BstNSI RCATGY 2 cut(s) 102, 379
BstSCI CCNGG 4 cut(s) 229, 307, 308, 336
BstSLI GKGCMC 2 cut(s) 254, 275
BtsCI GGATG 2 cut(s) 195, 254
BveI ACCTGC 1 cut(s) 258
Cac8I GCNNGC 2 cut(s) 275, 284
Cfr10I RCCGGY 1 cut(s) 316
Cfr13I GGNCC 1 cut(s) 227
Cfr9I CCCGGG 1 cut(s) 308
CseI GACGC 1 cut(s) 322
Csp6I GTAC 5 cut(s) 96, 117, 217, 331, 373
CviAII CATG 3 cut(s) 99, 376, 381
CviJI RGCY 7 cut(s) 48, 84, 128, 204, 209, 286, 344
CviKI_1 RGCY 7 cut(s) 48, 84, 128, 204, 209, 286, 344
CviQI GTAC 5 cut(s) 96, 117, 217, 331, 373
DdeI CTNAG 1 cut(s) 221
DpnI GATC 1 cut(s) 235
DpnII GATC 1 cut(s) 233
DraIII CACNNNGTG 1 cut(s) 279
EciI GGCGGA 1 cut(s) 341
Eco47I GGWCC 1 cut(s) 227
Eco72I CACGTG 1 cut(s) 295
Eco88I CYCGRG 1 cut(s) 308
EcoRII CCWGG 1 cut(s) 229
FaeI CATG 3 cut(s) 102, 379, 384
FaiI YATR 5 cut(s) 63, 100, 142, 377, 382
FaqI GGGAC 1 cut(s) 325
FatI CATG 3 cut(s) 98, 375, 380
Fnu4HI GCNGC 2 cut(s) 242, 265
FokI GGATG 2 cut(s) 202, 241
Fsp4HI GCNGC 2 cut(s) 242, 265
FspBI CTAG 1 cut(s) 210
GluI GCNGC 2 cut(s) 242, 265
HapII CCGG 3 cut(s) 309, 317, 338
HgaI GACGC 1 cut(s) 322
Hin1I GRCGYC 1 cut(s) 314
Hin1II CATG 3 cut(s) 102, 379, 384
HinfI GANTC 1 cut(s) 137
HpaII CCGG 3 cut(s) 309, 317, 338
HphI GGTGA 4 cut(s) 28, 64, 179, 331
Hpy166II GTNNAC 6 cut(s) 36, 117, 227, 252, 273, 292
Hpy188III TCNNGA 1 cut(s) 149
Hpy8I GTNNAC 6 cut(s) 36, 117, 227, 252, 273, 292
Hpy99I CGWCG 1 cut(s) 241
HpyAV CCTTC 2 cut(s) 4, 130
HpyCH4III ACNGT 1 cut(s) 167
HpyCH4IV ACGT 1 cut(s) 294
HpyCH4V TGCA 4 cut(s) 65, 252, 273, 282
HpyF10VI GCNNNNNNNGC 3 cut(s) 270, 279, 283
HpyF3I CTNAG 1 cut(s) 221
HpySE526I ACGT 1 cut(s) 294
Hsp92I GRCGYC 1 cut(s) 314
Hsp92II CATG 3 cut(s) 102, 379, 384
KpnI GGTACC 1 cut(s) 220
Kzo9I GATC 1 cut(s) 233
LmnI GCTCC 2 cut(s) 89, 181
LweI GCATC 1 cut(s) 183
MaeI CTAG 1 cut(s) 210
MaeII ACGT 1 cut(s) 294
MaeIII GTNAC 1 cut(s) 167
MalI GATC 1 cut(s) 235
MboI GATC 1 cut(s) 233
MhlI GDGCHC 2 cut(s) 254, 275
MluCI AATT 3 cut(s) 57, 152, 368
MnlI CCTC 6 cut(s) 171, 180, 207, 255, 334, 342
MslI CAYNNNNRTG 1 cut(s) 259
MspI CCGG 3 cut(s) 309, 317, 338
MspR9I CCNGG 4 cut(s) 231, 309, 310, 338
MvaI CCWGG 1 cut(s) 231
MwoI GCNNNNNNNGC 3 cut(s) 270, 279, 283
NciI CCSGG 3 cut(s) 309, 310, 338
NdeII GATC 1 cut(s) 233
NlaIII CATG 3 cut(s) 102, 379, 384
NlaIV GGNNCC 1 cut(s) 218
NmuCI GTSAC 1 cut(s) 167
NspI RCATGY 2 cut(s) 102, 379
PaqCI CACCTGC 1 cut(s) 258
PfeI GAWTC 1 cut(s) 137
PkrI GCNGC 2 cut(s) 243, 266
PmaCI CACGTG 1 cut(s) 295
PmlI CACGTG 1 cut(s) 295
Ppu21I YACGTR 1 cut(s) 295
Psp6I CCWGG 1 cut(s) 229
PspCI CACGTG 1 cut(s) 295
PspGI CCWGG 1 cut(s) 229
PspN4I GGNNCC 1 cut(s) 218
PspPI GGNCC 1 cut(s) 227
RsaI GTAC 5 cut(s) 97, 118, 218, 332, 374
RsaNI GTAC 5 cut(s) 96, 117, 217, 331, 373
RseI CAYNNNNRTG 1 cut(s) 259
SatI GCNGC 2 cut(s) 242, 265
Sau3AI GATC 1 cut(s) 233
Sau96I GGNCC 1 cut(s) 227
ScaI AGTACT 1 cut(s) 332
ScrFI CCNGG 4 cut(s) 231, 309, 310, 338
SduI GDGCHC 2 cut(s) 254, 275
SfaNI GCATC 1 cut(s) 183
SgrAI CRCCGGYG 1 cut(s) 316
SinI GGWCC 1 cut(s) 227
SmaI CCCGGG 1 cut(s) 310
SmiMI CAYNNNNRTG 1 cut(s) 259
SmlI CTYRAG 1 cut(s) 199
SmoI CTYRAG 1 cut(s) 199
Sse9I AATT 3 cut(s) 57, 152, 368
SsiI CCGC 4 cut(s) 39, 242, 264, 326
SspMI CTAG 1 cut(s) 210
StyD4I CCNGG 4 cut(s) 229, 307, 308, 336
TaaI ACNGT 1 cut(s) 167
TaiI ACGT 1 cut(s) 297
TaqI TCGA 1 cut(s) 135
TasI AATT 3 cut(s) 57, 152, 368
TatI WGTACW 3 cut(s) 95, 330, 372
TauI GCSGC 2 cut(s) 244, 267
TfiI GAWTC 1 cut(s) 137
TseFI GTSAC 1 cut(s) 167
Tsp45I GTSAC 1 cut(s) 167
TspDTI ATGAA 1 cut(s) 129
TspMI CCCGGG 1 cut(s) 308
VneI GTGCAC 2 cut(s) 250, 271
VpaK11BI GGWCC 1 cut(s) 227
XapI RAATTY 1 cut(s) 152
XceI RCATGY 2 cut(s) 102, 379
XmaI CCCGGG 1 cut(s) 308
XspI CTAG 1 cut(s) 210
ZrmI AGTACT 1 cut(s) 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.