Rh5AG475700

EPIDERMAL PATTERNING FACTOR-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0010795
Physical Location & Seq
Reverse (-)
81743251 .. 81744125
875 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG475700.1

Sequence Viewer

Length: 234 bp
ATGCATGTACAGGAGGAGCGGAGGGGGGAGAAGGCGATGGTGCTGCCTTCGAGCTTGGCGAGGAGGTACTTGAGCGGGCCCGGATCGTCCCCGCCTCGCTGCACGTCCAAGTGCGGCAGGTGCACGCCGTGCAAGCCGGTTCACGTGCCCGTGCCGCCCGGAACTCCGGTGACAGCCGAGTACTACCCGGAAGCGTGGAGGTGCAAGTGTGGGAACAAGTTGTACATGCCATGA

Protein Analysis

77

Amino Acids

8.53

Weight (kDa)

9.38

Isoelectric Point (pI)

90.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPF PF17181 27 - 77 3.1e-21 Epidermal patterning factor proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010795)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G30370 AT2G30370 AT2G30370
fragaria_vesca FvH4_3g40280 FvH4_3g40280 FvH4_6g29861
malus_domestica MD11G1055500.v1.1
prunus_persica Prupe.6G043000_v2.0.a1
pyrus_communis pycom03g04280 pycom11g04530
rosa_chinensis RchiOBHm_Chr5g0072621
rosa_laevigata RLG00000036317
rosa_multiflora Rmu_sc0007873.1_g000018
rosa_roxburghii Rroxscaffold_1G00008570 Rroxscaffold_2G00108910
rosa_rugosa Rorug02G0326300 Rorug05G0418900
rosa_samantha Rh5AG475700 Rh5DG508300
rosa_wichuraiana Rw5G044170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 108
Acc36I ACCTGC 1 cut(s) 108
AccBSI CCGCTC 2 cut(s) 19, 75
AciI CCGC 5 cut(s) 19, 75, 92, 114, 155
AclWI GGATC 1 cut(s) 91
AcvI CACGTG 1 cut(s) 145
AdeI CACNNNGTG 1 cut(s) 129
AfaI GTAC 4 cut(s) 9, 68, 182, 224
AjiI CACGTC 1 cut(s) 105
AluBI AGCT 1 cut(s) 54
AluI AGCT 1 cut(s) 54
Alw21I GWGCWC 1 cut(s) 125
Alw44I GTGCAC 1 cut(s) 121
AlwI GGATC 1 cut(s) 91
AoxI GGCC 1 cut(s) 77
ApaI GGGCCC 1 cut(s) 81
ApaLI GTGCAC 1 cut(s) 121
ApeKI GCWGC 2 cut(s) 43, 99
AspS9I GGNCC 2 cut(s) 77, 78
AsuC2I CCSGG 3 cut(s) 81, 159, 188
AsuHPI GGTGA 1 cut(s) 181
BaeGI GKGCMC 3 cut(s) 81, 125, 150
BanII GRGCYC 1 cut(s) 81
BbrPI CACGTG 1 cut(s) 145
Bbv12I GWGCWC 1 cut(s) 125
BbvI GCAGC 2 cut(s) 30, 86
BccI CCATC 1 cut(s) 31
BceAI ACGGC 1 cut(s) 112
BcgI CGANNNNNNTGC 2 cut(s) 25, 59
BcnI CCSGG 3 cut(s) 81, 159, 188
BfuAI ACCTGC 1 cut(s) 108
BisI GCNGC 4 cut(s) 44, 100, 115, 155
BlsI GCNGC 4 cut(s) 45, 101, 116, 156
BmcAI AGTACT 1 cut(s) 182
Bme1390I CCNGG 3 cut(s) 81, 159, 188
BmgBI CACGTC 1 cut(s) 105
BmgT120I GGNCC 2 cut(s) 77, 78
BmiI GGNNCC 1 cut(s) 79
BmrFI CCNGG 3 cut(s) 81, 159, 188
BpuEI CTTGAG 1 cut(s) 91
BpuMI CCSGG 3 cut(s) 81, 159, 188
BsaAI YACGTR 1 cut(s) 145
BsaWI WCCGGW 1 cut(s) 166
Bse118I RCCGGY 1 cut(s) 136
BseRI GAGGAG 2 cut(s) 29, 76
BseSI GKGCMC 3 cut(s) 81, 125, 150
BseXI GCAGC 2 cut(s) 30, 86
BsgI GTGCAG 1 cut(s) 85
BshFI GGCC 1 cut(s) 79
BsiHKAI GWGCWC 1 cut(s) 125
BsiSI CCGG 5 cut(s) 81, 137, 159, 167, 188
BslFI GGGAC 1 cut(s) 73
BsmFI GGGAC 1 cut(s) 73
BsnI GGCC 1 cut(s) 79
Bsp120I GGGCCC 1 cut(s) 77
Bsp1286I GDGCHC 3 cut(s) 81, 125, 150
Bsp1407I TGTACA 2 cut(s) 7, 222
Bsp143I GATC 1 cut(s) 83
BspACI CCGC 5 cut(s) 19, 75, 92, 114, 155
BspANI GGCC 1 cut(s) 79
BspLI GGNNCC 1 cut(s) 79
BspMI ACCTGC 1 cut(s) 108
BspPI GGATC 1 cut(s) 91
BsrBI CCGCTC 2 cut(s) 19, 75
BsrFI RCCGGY 1 cut(s) 136
BsrGI TGTACA 2 cut(s) 7, 222
BssAI RCCGGY 1 cut(s) 136
BssMI GATC 1 cut(s) 83
BstAPI GCANNNNNTGC 1 cut(s) 129
BstAUI TGTACA 2 cut(s) 7, 222
BstBAI YACGTR 1 cut(s) 145
BstC8I GCNNGC 3 cut(s) 77, 125, 134
BstKTI GATC 1 cut(s) 86
BstMBI GATC 1 cut(s) 83
BstMWI GCNNNNNNNGC 4 cut(s) 120, 129, 133, 154
BstNSI RCATGY 2 cut(s) 8, 229
BstSCI CCNGG 3 cut(s) 79, 157, 186
BstSLI GKGCMC 3 cut(s) 81, 125, 150
BstV1I GCAGC 2 cut(s) 30, 86
BsuRI GGCC 1 cut(s) 79
BtgZI GCGATG 1 cut(s) 50
BtrI CACGTC 1 cut(s) 105
BveI ACCTGC 1 cut(s) 108
Cac8I GCNNGC 3 cut(s) 77, 125, 134
Cfr10I RCCGGY 1 cut(s) 136
Cfr13I GGNCC 2 cut(s) 77, 78
Csp6I GTAC 4 cut(s) 8, 67, 181, 223
CviAII CATG 3 cut(s) 5, 226, 231
CviJI RGCY 4 cut(s) 54, 79, 136, 176
CviKI_1 RGCY 4 cut(s) 54, 79, 136, 176
CviQI GTAC 4 cut(s) 8, 67, 181, 223
DpnI GATC 1 cut(s) 85
DpnII GATC 1 cut(s) 83
DraIII CACNNNGTG 1 cut(s) 129
Eco24I GRGCYC 1 cut(s) 81
Eco72I CACGTG 1 cut(s) 145
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 81
FaeI CATG 3 cut(s) 8, 229, 234
FaiI YATR 3 cut(s) 6, 227, 232
FaqI GGGAC 1 cut(s) 73
FatI CATG 3 cut(s) 4, 225, 230
FauI CCCGC 2 cut(s) 68, 99
Fnu4HI GCNGC 4 cut(s) 44, 100, 115, 155
FriOI GRGCYC 1 cut(s) 81
Fsp4HI GCNGC 4 cut(s) 44, 100, 115, 155
GluI GCNGC 4 cut(s) 44, 100, 115, 155
HaeIII GGCC 1 cut(s) 79
HapII CCGG 5 cut(s) 81, 137, 159, 167, 188
Hin1II CATG 3 cut(s) 8, 229, 234
HpaII CCGG 5 cut(s) 81, 137, 159, 167, 188
HphI GGTGA 1 cut(s) 181
Hpy166II GTNNAC 2 cut(s) 123, 142
Hpy8I GTNNAC 2 cut(s) 123, 142
HpyAV CCTTC 2 cut(s) 25, 57
HpyCH4IV ACGT 2 cut(s) 104, 144
HpyCH4V TGCA 5 cut(s) 4, 102, 123, 132, 204
HpyF10VI GCNNNNNNNGC 4 cut(s) 120, 129, 133, 154
HpySE526I ACGT 2 cut(s) 104, 144
Hsp92II CATG 3 cut(s) 8, 229, 234
Kzo9I GATC 1 cut(s) 83
LmnI GCTCC 1 cut(s) 16
LpnPI CCDG 6 cut(s) 94, 103, 150, 172, 180, 201
Lsp1109I GCAGC 2 cut(s) 30, 86
MaeII ACGT 2 cut(s) 104, 144
MaeIII GTNAC 1 cut(s) 169
MalI GATC 1 cut(s) 85
MbiI CCGCTC 2 cut(s) 19, 75
MboI GATC 1 cut(s) 83
MhlI GDGCHC 3 cut(s) 81, 125, 150
MnlI CCTC 6 cut(s) 7, 15, 54, 57, 105, 192
Mph1103I ATGCAT 1 cut(s) 6
MspI CCGG 5 cut(s) 81, 137, 159, 167, 188
MspR9I CCNGG 3 cut(s) 81, 159, 188
MwoI GCNNNNNNNGC 4 cut(s) 120, 129, 133, 154
NciI CCSGG 3 cut(s) 81, 159, 188
NdeII GATC 1 cut(s) 83
NlaIII CATG 3 cut(s) 8, 229, 234
NlaIV GGNNCC 1 cut(s) 79
NmeAIII GCCGAG 1 cut(s) 202
NmuCI GTSAC 1 cut(s) 169
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 2 cut(s) 8, 229
PaqCI CACCTGC 1 cut(s) 108
PcsI WCGNNNNNNNCGW 1 cut(s) 56
PkrI GCNGC 4 cut(s) 45, 101, 116, 156
PmaCI CACGTG 1 cut(s) 145
PmlI CACGTG 1 cut(s) 145
Ppu21I YACGTR 1 cut(s) 145
PspCI CACGTG 1 cut(s) 145
PspN4I GGNNCC 1 cut(s) 79
PspOMI GGGCCC 1 cut(s) 77
PspPI GGNCC 2 cut(s) 77, 78
PsrI GAACNNNNNNTAC 1 cut(s) 206
RsaI GTAC 4 cut(s) 9, 68, 182, 224
RsaNI GTAC 4 cut(s) 8, 67, 181, 223
SatI GCNGC 4 cut(s) 44, 100, 115, 155
Sau3AI GATC 1 cut(s) 83
Sau96I GGNCC 2 cut(s) 77, 78
ScaI AGTACT 1 cut(s) 182
ScrFI CCNGG 3 cut(s) 81, 159, 188
SduI GDGCHC 3 cut(s) 81, 125, 150
SetI ASST 6 cut(s) 56, 68, 107, 122, 147, 203
SmlI CTYRAG 1 cut(s) 70
SmoI CTYRAG 1 cut(s) 70
SsiI CCGC 5 cut(s) 19, 75, 92, 114, 155
StyD4I CCNGG 3 cut(s) 79, 157, 186
TaiI ACGT 2 cut(s) 107, 147
TaqI TCGA 1 cut(s) 50
TatI WGTACW 3 cut(s) 7, 180, 222
TauI GCSGC 2 cut(s) 117, 157
TseFI GTSAC 1 cut(s) 169
TseI GCWGC 2 cut(s) 43, 99
Tsp45I GTSAC 1 cut(s) 169
VneI GTGCAC 1 cut(s) 121
XceI RCATGY 2 cut(s) 8, 229
ZrmI AGTACT 1 cut(s) 182
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.