MD11G1055500.v1.1

EPIDERMAL PATTERNING FACTOR-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0010795
Physical Location & Seq
Forward (+)
4716237 .. 4718200
1964 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1055500.v1.1.491

Sequence Viewer

Length: 384 bp
ATGGAGTTGAAAAGACTTCACAGACTAATAAGGTTCACCACCATCGGCATTTTCTCAATTTATGCACTTTTCACCACCATCAGCTCCACTTCCACTACATGTCCAGATAACAACTGTGCCTTCACGGGCAAACTGGATTCATATTTTCAGGAATTTGGGAAGCACAGCGACAAAATGAGGGAGCAGAGGATGATGACTTTGAGCTTGGCTAGGAGGTACTTGAGTGGACCTGGATCGTCGCCCCCTCGGTGCACATCCAAGTGCGGCAGGTGCACCCCTTGCAAGCCGGTTCACGTGTTTGTGCCACCTGGGACGCCGGTGATGGCGGAGTACTACCCGGAGGCTTGGAGGTGCAAATGTGGCAACAAGTTGTACATGCCGTGA

Protein Analysis

128

Amino Acids

14.45

Weight (kDa)

9.45

Isoelectric Point (pI)

48.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPF PF17181 77 - 127 8.8e-21 Epidermal patterning factor proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010795)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G30370 AT2G30370 AT2G30370
fragaria_vesca FvH4_3g40280 FvH4_3g40280 FvH4_6g29861
malus_domestica MD11G1055500.v1.1
prunus_persica Prupe.6G043000_v2.0.a1
pyrus_communis pycom03g04280 pycom11g04530
rosa_chinensis RchiOBHm_Chr5g0072621
rosa_laevigata RLG00000036317
rosa_multiflora Rmu_sc0007873.1_g000018
rosa_roxburghii Rroxscaffold_1G00008570 Rroxscaffold_2G00108910
rosa_rugosa Rorug02G0326300 Rorug05G0418900
rosa_samantha Rh5AG475700 Rh5DG508300
rosa_wichuraiana Rw5G044170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 258
Acc36I ACCTGC 1 cut(s) 258
AciI CCGC 2 cut(s) 264, 326
AclWI GGATC 1 cut(s) 241
AcsI RAATTY 1 cut(s) 152
AcvI CACGTG 1 cut(s) 295
AcyI GRCGYC 1 cut(s) 314
AfaI GTAC 3 cut(s) 218, 332, 374
AflIII ACRYGT 2 cut(s) 98, 294
AgsI TTSAA 1 cut(s) 10
AjnI CCWGG 2 cut(s) 229, 307
AluBI AGCT 2 cut(s) 84, 204
AluI AGCT 2 cut(s) 84, 204
Alw21I GWGCWC 2 cut(s) 254, 275
Alw44I GTGCAC 2 cut(s) 250, 271
AlwI GGATC 1 cut(s) 241
ApaLI GTGCAC 2 cut(s) 250, 271
ApoI RAATTY 1 cut(s) 152
AspS9I GGNCC 1 cut(s) 227
AsuC2I CCSGG 1 cut(s) 338
AsuHPI GGTGA 3 cut(s) 28, 64, 331
AvaII GGWCC 1 cut(s) 227
BaeGI GKGCMC 2 cut(s) 254, 275
BaeI ACNNNNGTAYC 2 cut(s) 208, 241
BbrPI CACGTG 1 cut(s) 295
Bbv12I GWGCWC 2 cut(s) 254, 275
BccI CCATC 3 cut(s) 50, 86, 316
BceAI ACGGC 1 cut(s) 364
BciT130I CCWGG 2 cut(s) 231, 309
BcnI CCSGG 1 cut(s) 338
BfaI CTAG 1 cut(s) 210
BfuAI ACCTGC 1 cut(s) 258
BisI GCNGC 1 cut(s) 265
BlsI GCNGC 1 cut(s) 266
BmcAI AGTACT 1 cut(s) 332
Bme1390I CCNGG 3 cut(s) 231, 309, 338
Bme18I GGWCC 1 cut(s) 227
BmgT120I GGNCC 1 cut(s) 227
BmrFI CCNGG 3 cut(s) 231, 309, 338
BpuEI CTTGAG 1 cut(s) 241
BpuMI CCSGG 1 cut(s) 338
BsaAI YACGTR 1 cut(s) 295
BsaHI GRCGYC 1 cut(s) 314
BsaJI CCNNGG 2 cut(s) 245, 308
Bse118I RCCGGY 2 cut(s) 286, 316
Bse1I ACTGG 1 cut(s) 138
BseBI CCWGG 2 cut(s) 231, 309
BseDI CCNNGG 2 cut(s) 245, 308
BseGI GGATG 2 cut(s) 195, 254
BseNI ACTGG 1 cut(s) 138
BseSI GKGCMC 2 cut(s) 254, 275
BsiHKAI GWGCWC 2 cut(s) 254, 275
BsiSI CCGG 3 cut(s) 287, 317, 338
BslFI GGGAC 1 cut(s) 325
BsmFI GGGAC 1 cut(s) 325
Bsp1286I GDGCHC 2 cut(s) 254, 275
Bsp1407I TGTACA 1 cut(s) 372
Bsp143I GATC 1 cut(s) 233
BspACI CCGC 2 cut(s) 264, 326
BspMI ACCTGC 1 cut(s) 258
BspPI GGATC 1 cut(s) 241
BsrFI RCCGGY 2 cut(s) 286, 316
BsrGI TGTACA 1 cut(s) 372
BsrI ACTGG 1 cut(s) 138
BssAI RCCGGY 2 cut(s) 286, 316
BssECI CCNNGG 2 cut(s) 245, 308
BssMI GATC 1 cut(s) 233
BssNI GRCGYC 1 cut(s) 314
Bst2UI CCWGG 2 cut(s) 231, 309
Bst4CI ACNGT 1 cut(s) 116
BstACI GRCGYC 1 cut(s) 314
BstAPI GCANNNNNTGC 1 cut(s) 279
BstAUI TGTACA 1 cut(s) 372
BstBAI YACGTR 1 cut(s) 295
BstC8I GCNNGC 1 cut(s) 284
BstF5I GGATG 2 cut(s) 195, 254
BstKTI GATC 1 cut(s) 236
BstMBI GATC 1 cut(s) 233
BstMWI GCNNNNNNNGC 3 cut(s) 270, 279, 360
BstNI CCWGG 2 cut(s) 231, 309
BstNSI RCATGY 2 cut(s) 102, 379
BstSCI CCNGG 3 cut(s) 229, 307, 336
BstSLI GKGCMC 2 cut(s) 254, 275
BtsCI GGATG 2 cut(s) 195, 254
BveI ACCTGC 1 cut(s) 258
Cac8I GCNNGC 1 cut(s) 284
Cfr10I RCCGGY 2 cut(s) 286, 316
Cfr13I GGNCC 1 cut(s) 227
CseI GACGC 1 cut(s) 322
Csp6I GTAC 3 cut(s) 217, 331, 373
CviAII CATG 2 cut(s) 99, 376
CviJI RGCY 5 cut(s) 84, 204, 209, 286, 344
CviKI_1 RGCY 5 cut(s) 84, 204, 209, 286, 344
CviQI GTAC 3 cut(s) 217, 331, 373
DpnI GATC 1 cut(s) 235
DpnII GATC 1 cut(s) 233
EciI GGCGGA 1 cut(s) 341
Eco47I GGWCC 1 cut(s) 227
Eco72I CACGTG 1 cut(s) 295
EcoRII CCWGG 2 cut(s) 229, 307
FaeI CATG 2 cut(s) 102, 379
FaiI YATR 4 cut(s) 63, 100, 142, 377
FaqI GGGAC 1 cut(s) 325
FatI CATG 2 cut(s) 98, 375
Fnu4HI GCNGC 1 cut(s) 265
FokI GGATG 2 cut(s) 202, 241
Fsp4HI GCNGC 1 cut(s) 265
FspBI CTAG 1 cut(s) 210
GluI GCNGC 1 cut(s) 265
HapII CCGG 3 cut(s) 287, 317, 338
HgaI GACGC 1 cut(s) 322
Hin1I GRCGYC 1 cut(s) 314
Hin1II CATG 2 cut(s) 102, 379
HinfI GANTC 1 cut(s) 137
HpaII CCGG 3 cut(s) 287, 317, 338
HphI GGTGA 3 cut(s) 28, 64, 331
Hpy166II GTNNAC 5 cut(s) 36, 227, 252, 273, 292
Hpy188III TCNNGA 2 cut(s) 104, 149
Hpy8I GTNNAC 5 cut(s) 36, 227, 252, 273, 292
Hpy99I CGWCG 1 cut(s) 241
HpyAV CCTTC 1 cut(s) 130
HpyCH4III ACNGT 1 cut(s) 116
HpyCH4IV ACGT 1 cut(s) 294
HpyCH4V TGCA 5 cut(s) 65, 252, 273, 282, 354
HpyF10VI GCNNNNNNNGC 3 cut(s) 270, 279, 360
HpySE526I ACGT 1 cut(s) 294
Hsp92I GRCGYC 1 cut(s) 314
Hsp92II CATG 2 cut(s) 102, 379
Kzo9I GATC 1 cut(s) 233
LmnI GCTCC 2 cut(s) 89, 181
MaeI CTAG 1 cut(s) 210
MaeII ACGT 1 cut(s) 294
MalI GATC 1 cut(s) 235
MboI GATC 1 cut(s) 233
MhlI GDGCHC 2 cut(s) 254, 275
MluCI AATT 2 cut(s) 57, 152
MnlI CCTC 6 cut(s) 171, 180, 207, 255, 334, 342
MslI CAYNNNNRTG 1 cut(s) 259
MspI CCGG 3 cut(s) 287, 317, 338
MspR9I CCNGG 3 cut(s) 231, 309, 338
MvaI CCWGG 2 cut(s) 231, 309
MwoI GCNNNNNNNGC 3 cut(s) 270, 279, 360
NciI CCSGG 1 cut(s) 338
NdeII GATC 1 cut(s) 233
NlaIII CATG 2 cut(s) 102, 379
NspI RCATGY 2 cut(s) 102, 379
PaqCI CACCTGC 1 cut(s) 258
PciI ACATGT 1 cut(s) 98
PfeI GAWTC 1 cut(s) 137
PkrI GCNGC 1 cut(s) 266
PmaCI CACGTG 1 cut(s) 295
PmlI CACGTG 1 cut(s) 295
Ppu21I YACGTR 1 cut(s) 295
PscI ACATGT 1 cut(s) 98
Psp6I CCWGG 2 cut(s) 229, 307
PspCI CACGTG 1 cut(s) 295
PspGI CCWGG 2 cut(s) 229, 307
PspPI GGNCC 1 cut(s) 227
RsaI GTAC 3 cut(s) 218, 332, 374
RsaNI GTAC 3 cut(s) 217, 331, 373
RseI CAYNNNNRTG 1 cut(s) 259
SatI GCNGC 1 cut(s) 265
Sau3AI GATC 1 cut(s) 233
Sau96I GGNCC 1 cut(s) 227
ScaI AGTACT 1 cut(s) 332
ScrFI CCNGG 3 cut(s) 231, 309, 338
SduI GDGCHC 2 cut(s) 254, 275
SetI ASST 9 cut(s) 35, 86, 206, 218, 232, 272, 297, 310, 353
SgrAI CRCCGGYG 1 cut(s) 316
SinI GGWCC 1 cut(s) 227
SmiMI CAYNNNNRTG 1 cut(s) 259
SmlI CTYRAG 1 cut(s) 220
SmoI CTYRAG 1 cut(s) 220
Sse9I AATT 2 cut(s) 57, 152
SsiI CCGC 2 cut(s) 264, 326
SspMI CTAG 1 cut(s) 210
StyD4I CCNGG 3 cut(s) 229, 307, 336
TaaI ACNGT 1 cut(s) 116
TaiI ACGT 1 cut(s) 297
TasI AATT 2 cut(s) 57, 152
TatI WGTACW 2 cut(s) 330, 372
TauI GCSGC 1 cut(s) 267
TfiI GAWTC 1 cut(s) 137
TspDTI ATGAA 1 cut(s) 129
VneI GTGCAC 2 cut(s) 250, 271
VpaK11BI GGWCC 1 cut(s) 227
XapI RAATTY 1 cut(s) 152
XceI RCATGY 2 cut(s) 102, 379
XspI CTAG 1 cut(s) 210
ZrmI AGTACT 1 cut(s) 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.