FvH4_3g42500

membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0010952
Physical Location & Seq
Forward (+)
35430003 .. 35433056
3054 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g42500.t2

Sequence Viewer

Length: 1182 bp
ATGATCCCACATATATGCCTGTGCCATTGTCAGATTGGAATGGCTTTGCTTGATTCATTGTTGGGGAATGAAGCTAAAAGATTTGTGAAAAGGAAAGACAGTGATGCAGGAGAAGCAGGTAAAGCACTTGAAGAACTCAGAGGCTCTCTCTACAATGACCTTCGAACTTCAGAAGGCGCCAAGCGCCAACAGCAACGATTTTGTGGTCCGGTGGTGGCGATGACCTTCAATTTTGTGGTGTCTGTGGGGATCATCCTGGCAAATAAACTTGTAATGGGGAGAGTTGGATTCAAGTTTCCAATCTTTCTCACATTGATTCATTATGTGACAGCTTGGTTGCTTCTAGCCATTTTTAAGACACTCTCAATTCTTCCGGTTTCTCCTCCGGCCAGAACTACCCCTTTCACTTCTCTCTTCTCTTTAGGTGCTGTCATGGCTTTTGCCTCTGGTCTTGCCAATACAAGTCTAAAACACAACAGTGTTGGTTTCTACCAGATGGCTAAAATTGCTGTAACTCCTACCATTGTTCTTGCCGAGTTTATTCTCTTTAGAAAAACCATTTCTTGTAACAAGGTTTTAGCTCTAGCTGTCGTCTCAGCAGGTGTGGCAGTAGCAACAGTAACAGATTTAGAGTTCAATTTGTTTGGTGCCTTGATTGCAATCGCGTGGATAGTCCCAAGTGCTGTAAACAAAATCCTGTGGTCTAATCTTCAACAGCAAGGGAATTGGACCGCCCTCGCGCTGATGTGGAGGACTACCCCAGTCACTATTTTCTTCTTATTAGCTCTTATGCCATGGTTGGATCCACCAGGAGTTCTACTGTTCCAGTGGAATATGAATAACTCAAGTGCTATTCTTATATCAGCAATCCTTGGTTTTCTCCTCCAATGGTCCGGGGCATTGGCACTCGGGGCAACCTCAGCAACTAGCCATGTTGTTCTTGGACAGTTCAAGACTTGTGTGATATTACTAGGGGGATATTTTCTTTTCAAATCAGATCCAGGGTTCGTGAGCCTCTGTGGGGCTGTTACAGCCCTCGGTGGAATGTCTGTTTACACATCACTAAACCTGAAAAAACCACAAGACAATGTGACCAAGCAATCCTTACCTACACTAAAACCCAAAACCATAGACCAAGAAAGTGTAGTAGAGTCAGAAGCAAAGGATACCACAGTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPT

Protein Analysis

394

Amino Acids

42.51

Weight (kDa)

9.38

Isoelectric Point (pI)

37.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPT PF03151 75 - 265 2.6e-11 Triose-phosphate Transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010952)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55950 AT5G55950 AT5G55950 AT5G55950
fragaria_vesca FvH4_3g42500 FvH4_3g42500
malus_domestica MD11G1035900.v1.1
prunus_persica Prupe.6G026800_v2.0.a1 Prupe.6G026800_v2.0.a1
pyrus_communis pycom11g02970
rosa_chinensis RchiOBHm_Chr5g0076161
rosa_laevigata RLG00000036606
rosa_multiflora Rmu_ssc0000123.1_g000002
rosa_roxburghii Rroxscaffold_1G00005650
rosa_rugosa Rorug05G0445200
rosa_samantha Rh5AG501300 Rh5BG522800 Rh5CG546600 Rh5DG535200
rosa_wichuraiana Rw5G046530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 590
Acc36I ACCTGC 2 cut(s) 107, 590
AccB1I GGYRCC 2 cut(s) 176, 647
AccII CGCG 2 cut(s) 665, 740
AciI CCGC 1 cut(s) 732
AclWI GGATC 4 cut(s) 257, 797, 810, 992
AcoI YGGCCR 1 cut(s) 387
AcuI CTGAAG 1 cut(s) 153
AcyI GRCGYC 1 cut(s) 177
AfiI CCNNNNNNNGG 1 cut(s) 1021
AgsI TTSAA 7 cut(s) 131, 229, 292, 637, 713, 952, 991
AjnI CCWGG 3 cut(s) 255, 808, 1000
AleI CACNNNNGTG 1 cut(s) 477
AluBI AGCT 5 cut(s) 74, 332, 581, 587, 785
AluI AGCT 5 cut(s) 74, 332, 581, 587, 785
Alw26I GTCTC 1 cut(s) 598
AlwI GGATC 4 cut(s) 257, 797, 810, 992
Ama87I CYCGRG 1 cut(s) 908
AoxI GGCC 1 cut(s) 387
AspLEI GCGC 3 cut(s) 179, 186, 742
AspS9I GGNCC 3 cut(s) 206, 729, 891
AsuC2I CCSGG 1 cut(s) 895
AsuII TTCGAA 1 cut(s) 163
AvaI CYCGRG 1 cut(s) 908
AvaII GGWCC 3 cut(s) 206, 729, 891
BaeI ACNNNNGTAYC 1 cut(s) 1158
BamHI GGATCC 1 cut(s) 802
BanI GGYRCC 2 cut(s) 176, 647
BbvCI CCTCAGC 1 cut(s) 919
BccI CCATC 1 cut(s) 490
BciT130I CCWGG 3 cut(s) 257, 810, 1002
BciVI GTATCC 1 cut(s) 1159
BcnI CCSGG 1 cut(s) 895
BcoDI GTCTC 1 cut(s) 598
BfaI CTAG 4 cut(s) 344, 584, 927, 971
BfoI RGCGCY 2 cut(s) 180, 187
BfuAI ACCTGC 2 cut(s) 107, 590
BfuI GTATCC 1 cut(s) 1159
Bme1390I CCNGG 4 cut(s) 257, 810, 895, 1002
Bme18I GGWCC 3 cut(s) 206, 729, 891
BmeT110I CYCGRG 1 cut(s) 908
BmgT120I GGNCC 3 cut(s) 206, 729, 891
BmiI GGNNCC 3 cut(s) 178, 649, 804
BmrFI CCNGG 4 cut(s) 257, 810, 895, 1002
BmrI ACTGGG 1 cut(s) 755
BmsI GCATC 1 cut(s) 94
BmuI ACTGGG 1 cut(s) 755
BplI GAGNNNNNCTC 2 cut(s) 132, 164
Bpu10I CCTNAGC 1 cut(s) 919
Bpu14I TTCGAA 1 cut(s) 163
BpuEI CTTGAG 1 cut(s) 829
BpuMI CCSGG 1 cut(s) 895
BsaBI GATNNNNATC 1 cut(s) 659
BsaHI GRCGYC 1 cut(s) 177
BsaJI CCNNGG 5 cut(s) 794, 871, 894, 1001, 1036
BsaWI WCCGGW 2 cut(s) 208, 373
Bsc4I CCNNNNNNNGG 1 cut(s) 1021
Bse1I ACTGG 2 cut(s) 761, 826
Bse8I GATNNNNATC 1 cut(s) 659
BseBI CCWGG 3 cut(s) 257, 810, 1002
BseDI CCNNGG 5 cut(s) 794, 871, 894, 1001, 1036
BseGI GGATG 1 cut(s) 252
BseJI GATNNNNATC 1 cut(s) 659
BseLI CCNNNNNNNGG 1 cut(s) 1021
BseMII CTCAG 3 cut(s) 151, 609, 933
BseNI ACTGG 2 cut(s) 761, 826
BseRI GAGGAG 2 cut(s) 372, 872
Bsh1236I CGCG 2 cut(s) 665, 740
BshFI GGCC 1 cut(s) 389
BshNI GGYRCC 2 cut(s) 176, 647
BsiHKCI CYCGRG 1 cut(s) 908
BsiSI CCGG 4 cut(s) 209, 374, 386, 894
BslFI GGGAC 1 cut(s) 659
BslI CCNNNNNNNGG 1 cut(s) 1021
BsmAI GTCTC 1 cut(s) 598
BsmBI CGTCTC 1 cut(s) 598
BsmFI GGGAC 1 cut(s) 659
BsnI GGCC 1 cut(s) 389
BsoBI CYCGRG 1 cut(s) 908
Bsp119I TTCGAA 1 cut(s) 163
Bsp143I GATC 4 cut(s) 3, 249, 802, 997
Bsp19I CCATGG 1 cut(s) 794
BspACI CCGC 1 cut(s) 732
BspANI GGCC 1 cut(s) 389
BspCNI CTCAG 3 cut(s) 150, 608, 932
BspFNI CGCG 2 cut(s) 665, 740
BspLI GGNNCC 3 cut(s) 178, 649, 804
BspMI ACCTGC 2 cut(s) 107, 590
BspPI GGATC 4 cut(s) 257, 797, 810, 992
BspT104I TTCGAA 1 cut(s) 163
BspT107I GGYRCC 2 cut(s) 176, 647
BsrI ACTGG 2 cut(s) 761, 826
BssECI CCNNGG 5 cut(s) 794, 871, 894, 1001, 1036
BssMI GATC 4 cut(s) 3, 249, 802, 997
BssNI GRCGYC 1 cut(s) 177
BssT1I CCWWGG 2 cut(s) 794, 871
Bst2UI CCWGG 3 cut(s) 257, 810, 1002
Bst4CI ACNGT 6 cut(s) 101, 479, 619, 822, 948, 1174
Bst6I CTCTTC 1 cut(s) 419
BstACI GRCGYC 1 cut(s) 177
BstBI TTCGAA 1 cut(s) 163
BstDEI CTNAG 3 cut(s) 137, 595, 919
BstDSI CCRYGG 1 cut(s) 794
BstF5I GGATG 1 cut(s) 252
BstFNI CGCG 2 cut(s) 665, 740
BstH2I RGCGCY 2 cut(s) 180, 187
BstHHI GCGC 3 cut(s) 179, 186, 742
BstKTI GATC 4 cut(s) 6, 252, 805, 1000
BstMAI GTCTC 1 cut(s) 598
BstMBI GATC 4 cut(s) 3, 249, 802, 997
BstNI CCWGG 3 cut(s) 257, 810, 1002
BstSCI CCNGG 4 cut(s) 255, 808, 893, 1000
BstUI CGCG 2 cut(s) 665, 740
BstX2I RGATCY 2 cut(s) 802, 997
BstYI RGATCY 2 cut(s) 802, 997
BsuI GTATCC 1 cut(s) 1159
BsuRI GGCC 1 cut(s) 389
BtgI CCRYGG 1 cut(s) 794
BtgZI GCGATG 1 cut(s) 233
BtsCI GGATG 1 cut(s) 252
BtsIMutI CAGTG 3 cut(s) 106, 484, 833
BveI ACCTGC 2 cut(s) 107, 590
CfoI GCGC 3 cut(s) 179, 186, 742
Cfr13I GGNCC 3 cut(s) 206, 729, 891
CviAII CATG 3 cut(s) 433, 795, 932
DdeI CTNAG 3 cut(s) 137, 595, 919
DinI GGCGCC 1 cut(s) 178
DpnI GATC 4 cut(s) 5, 251, 804, 999
DpnII GATC 4 cut(s) 3, 249, 802, 997
EaeI YGGCCR 1 cut(s) 387
Eam1104I CTCTTC 1 cut(s) 419
EarI CTCTTC 1 cut(s) 419
Eco130I CCWWGG 2 cut(s) 794, 871
Eco47I GGWCC 3 cut(s) 206, 729, 891
Eco57I CTGAAG 1 cut(s) 153
Eco88I CYCGRG 1 cut(s) 908
EcoRII CCWGG 3 cut(s) 255, 808, 1000
EcoT14I CCWWGG 2 cut(s) 794, 871
EgeI GGCGCC 1 cut(s) 178
EheI GGCGCC 1 cut(s) 178
ErhI CCWWGG 2 cut(s) 794, 871
Esp3I CGTCTC 1 cut(s) 598
FaeI CATG 3 cut(s) 436, 798, 935
FalI AAGNNNNNCTT 2 cut(s) 1088, 1120
FaqI GGGAC 1 cut(s) 659
FatI CATG 3 cut(s) 432, 794, 931
FokI GGATG 1 cut(s) 239
FspBI CTAG 4 cut(s) 344, 584, 927, 971
GlaI GCGC 3 cut(s) 178, 185, 741
HaeII RGCGCY 2 cut(s) 180, 187
HaeIII GGCC 1 cut(s) 389
HapII CCGG 4 cut(s) 209, 374, 386, 894
HhaI GCGC 3 cut(s) 179, 186, 742
Hin1I GRCGYC 1 cut(s) 177
Hin1II CATG 3 cut(s) 436, 798, 935
Hin6I GCGC 3 cut(s) 177, 184, 740
HinP1I GCGC 3 cut(s) 177, 184, 740
HinfI GANTC 4 cut(s) 53, 288, 316, 1151
HpaII CCGG 4 cut(s) 209, 374, 386, 894
Hpy166II GTNNAC 2 cut(s) 688, 1054
Hpy188I TCNGA 5 cut(s) 33, 140, 172, 997, 1156
Hpy188III TCNNGA 2 cut(s) 952, 1009
Hpy8I GTNNAC 2 cut(s) 688, 1054
HpyAV CCTTC 3 cut(s) 167, 170, 235
HpyCH4III ACNGT 6 cut(s) 101, 479, 619, 822, 948, 1174
HpyCH4V TGCA 2 cut(s) 107, 659
HpyF3I CTNAG 3 cut(s) 137, 595, 919
Hsp92I GRCGYC 1 cut(s) 177
Hsp92II CATG 3 cut(s) 436, 798, 935
HspAI GCGC 3 cut(s) 177, 184, 740
KasI GGCGCC 1 cut(s) 176
Kzo9I GATC 4 cut(s) 3, 249, 802, 997
LweI GCATC 1 cut(s) 94
MaeI CTAG 4 cut(s) 344, 584, 927, 971
MaeIII GTNAC 7 cut(s) 325, 511, 566, 619, 763, 1027, 1090
MalI GATC 4 cut(s) 5, 251, 804, 999
MboI GATC 4 cut(s) 3, 249, 802, 997
MboII GAAGA 5 cut(s) 143, 362, 406, 701, 766
MflI RGATCY 2 cut(s) 802, 997
MluCI AATT 5 cut(s) 229, 366, 504, 637, 724
Mly113I GGCGCC 1 cut(s) 177
MlyI GAGTC 1 cut(s) 1160
MmeI TCCRAC 2 cut(s) 265, 780
MnlI CCTC 9 cut(s) 134, 393, 454, 744, 746, 893, 928, 1025, 1046
MseI TTAA 1 cut(s) 354
MslI CAYNNNNRTG 2 cut(s) 13, 477
MspI CCGG 4 cut(s) 209, 374, 386, 894
MspR9I CCNGG 4 cut(s) 257, 810, 895, 1002
MvaI CCWGG 3 cut(s) 257, 810, 1002
MvnI CGCG 2 cut(s) 665, 740
NarI GGCGCC 1 cut(s) 177
NciI CCSGG 1 cut(s) 895
NcoI CCATGG 1 cut(s) 794
NdeII GATC 4 cut(s) 3, 249, 802, 997
NlaIII CATG 3 cut(s) 436, 798, 935
NlaIV GGNNCC 3 cut(s) 178, 649, 804
NmeAIII GCCGAG 1 cut(s) 559
NmuCI GTSAC 3 cut(s) 325, 763, 1090
NspV TTCGAA 1 cut(s) 163
OliI CACNNNNGTG 1 cut(s) 477
PaqCI CACCTGC 1 cut(s) 590
PfeI GAWTC 3 cut(s) 53, 288, 316
PleI GAGTC 1 cut(s) 1159
PluTI GGCGCC 1 cut(s) 180
PpsI GAGTC 1 cut(s) 1159
Psp6I CCWGG 3 cut(s) 255, 808, 1000
PspGI CCWGG 3 cut(s) 255, 808, 1000
PspN4I GGNNCC 3 cut(s) 178, 649, 804
PspPI GGNCC 3 cut(s) 206, 729, 891
PsuI RGATCY 2 cut(s) 802, 997
RseI CAYNNNNRTG 2 cut(s) 13, 477
SaqAI TTAA 1 cut(s) 354
Sau3AI GATC 4 cut(s) 3, 249, 802, 997
Sau96I GGNCC 3 cut(s) 206, 729, 891
SchI GAGTC 1 cut(s) 1160
ScrFI CCNGG 4 cut(s) 257, 810, 895, 1002
SfaNI GCATC 1 cut(s) 94
SfoI GGCGCC 1 cut(s) 178
SfuI TTCGAA 1 cut(s) 163
SinI GGWCC 3 cut(s) 206, 729, 891
SmiMI CAYNNNNRTG 2 cut(s) 13, 477
SmlI CTYRAG 1 cut(s) 844
SmoI CTYRAG 1 cut(s) 844
Sse9I AATT 5 cut(s) 229, 366, 504, 637, 724
SsiI CCGC 1 cut(s) 732
SspDI GGCGCC 1 cut(s) 176
SspMI CTAG 4 cut(s) 344, 584, 927, 971
StyD4I CCNGG 4 cut(s) 255, 808, 893, 1000
StyI CCWWGG 2 cut(s) 794, 871
TaaI ACNGT 6 cut(s) 101, 479, 619, 822, 948, 1174
TaqI TCGA 1 cut(s) 163
TasI AATT 5 cut(s) 229, 366, 504, 637, 724
TfiI GAWTC 3 cut(s) 53, 288, 316
Tru1I TTAA 1 cut(s) 354
Tru9I TTAA 1 cut(s) 354
TscAI CASTG 3 cut(s) 106, 484, 833
TseFI GTSAC 3 cut(s) 325, 763, 1090
Tsp45I GTSAC 3 cut(s) 325, 763, 1090
TspDTI ATGAA 4 cut(s) 45, 84, 308, 851
TspRI CASTG 3 cut(s) 106, 484, 833
VpaK11BI GGWCC 3 cut(s) 206, 729, 891
XcmI CCANNNNNNNNNTGG 2 cut(s) 32, 938
XspI CTAG 4 cut(s) 344, 584, 927, 971
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.