Rh5DG535200

membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0010952
Physical Location & Seq
Forward (+)
82573195 .. 82575983
2789 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG535200.1

Sequence Viewer

Length: 1146 bp
ATGGCTTTGCTTGATTCATTGTTGGGGAATGAAGCCAAGAGATTTGTGAAAAGGAAAGACAGTGATGCAGGAGAAGCAGGTAAAGCACTTGAAGAACTCAGAGGTTCTCTCTACAATGAGCTTAGAACTTCAGAAGGCGCCAAGCGCCAACAGCAACGATTTTGTGGCCCGGTGGTGGCGATGACCTTCAATTTCATGGTGTCTGTGGGGATCATTCTGACAAATAAACTTGTAATGGGGAGAGTTGGATTTAAGTTTCCAATCTTTCTCACATTGATTCACTATGTGACAGCATGGTTGCTTCTAGCCATTTTTAAGACGCTCTCAATTCTTCCAGTTTCTCCTCCGGCTAGAACTACTCCTTTCACTTCTCTCTTCTCTTTAGGTGCTGTCATGGCTTTTGCTTCTGGTCTTGCCAATACAAGCCTAAAACACAACAGTGTTGGTTTCTACCAGATGGCTAAAATTGCTGTCACTCCTACCATTGTTCTCGCGGAGTTCATTCTCTTCAGAAAAACCATTTCTTGTAACAAGGTTTTAGCTCTAGCTGTGGTCTCAGCAGGTGTGGCAGTAGCAACAGTAACAGATCTCGAGTTCAATTTGTTTGGCGCTATGATTGCAATCGCGTGGATAGTCCCAAGTGCTGTAAACAAAATCCTATGGTCTAATCTGCAACAGCAAGGCAATTGGACCGCCCTTGCGCTGATGTGGAAGACTACCCCAGTCACTATATTCTTCTTATTAGCTCTGATGCCATGGTTGGATCCACCAGGAGTTCTACTATTCCAGTGGGATATCAGTAACTCAAGCGCCATTCTTATATCAGCAATCCTTGGTTTTCTCCTCCAATGGTCCGGAGCATTGGCACTTGGGGCAACCTCAGCAACTAGCCATGTTGTTCTTGGACAGTTCAAAACTTGTGTGATATTACTAGGGGGCTATTTCCTTTTCAAATCAGATCCAGGGTTCGTGAGCCTTTGTGGGGCTGTTACAGCCCTCTGTGGAATGTCTGTTTACACATCACTAAACCTGAAAAAGCCACAAGACAATGTGACCAAGCAAACCTTACCGACATTAAAACCCAAAACCATTAACCAAGAAAATGTAGTAGAGTCAGAAACGGAGGGTACCCCAACTGTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPT

Protein Analysis

381

Amino Acids

41.23

Weight (kDa)

9.42

Isoelectric Point (pI)

33.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPT PF03151 62 - 339 1.9e-13 Triose-phosphate Transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010952)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55950 AT5G55950 AT5G55950 AT5G55950
fragaria_vesca FvH4_3g42500 FvH4_3g42500
malus_domestica MD11G1035900.v1.1
prunus_persica Prupe.6G026800_v2.0.a1 Prupe.6G026800_v2.0.a1
pyrus_communis pycom11g02970
rosa_chinensis RchiOBHm_Chr5g0076161
rosa_laevigata RLG00000036606
rosa_multiflora Rmu_ssc0000123.1_g000002
rosa_roxburghii Rroxscaffold_1G00005650
rosa_rugosa Rorug05G0445200
rosa_samantha Rh5AG501300 Rh5BG522800 Rh5CG546600 Rh5DG535200
rosa_wichuraiana Rw5G046530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 551
Acc36I ACCTGC 2 cut(s) 68, 551
Acc65I GGTACC 1 cut(s) 1127
AccB1I GGYRCC 2 cut(s) 137, 1127
AccII CGCG 2 cut(s) 494, 626
AccIII TCCGGA 1 cut(s) 854
AciI CCGC 2 cut(s) 494, 693
AclWI GGATC 4 cut(s) 218, 758, 771, 953
AcuI CTGAAG 2 cut(s) 114, 493
AcyI GRCGYC 1 cut(s) 138
AdeI CACNNNGTG 1 cut(s) 286
AfaI GTAC 1 cut(s) 1129
AfiI CCNNNNNNNGG 2 cut(s) 175, 982
AgsI TTSAA 5 cut(s) 92, 190, 598, 913, 952
AjnI CCWGG 2 cut(s) 769, 961
AleI CACNNNNGTG 1 cut(s) 438
AluBI AGCT 4 cut(s) 121, 542, 548, 746
AluI AGCT 4 cut(s) 121, 542, 548, 746
Alw26I GTCTC 1 cut(s) 559
AlwI GGATC 4 cut(s) 218, 758, 771, 953
Ama87I CYCGRG 1 cut(s) 590
Aor13HI TCCGGA 1 cut(s) 854
AoxI GGCC 1 cut(s) 166
Asp718I GGTACC 1 cut(s) 1127
AspLEI GCGC 5 cut(s) 140, 147, 611, 703, 812
AspS9I GGNCC 3 cut(s) 167, 690, 852
AsuC2I CCSGG 1 cut(s) 170
AvaI CYCGRG 1 cut(s) 590
AvaII GGWCC 2 cut(s) 690, 852
BamHI GGATCC 1 cut(s) 763
BanI GGYRCC 2 cut(s) 137, 1127
BbsI GAAGAC 1 cut(s) 719
BbvCI CCTCAGC 1 cut(s) 880
BccI CCATC 1 cut(s) 451
BciT130I CCWGG 2 cut(s) 771, 963
BcnI CCSGG 1 cut(s) 170
BcoDI GTCTC 1 cut(s) 559
BfaI CTAG 5 cut(s) 305, 351, 545, 888, 932
BfoI RGCGCY 4 cut(s) 141, 148, 612, 813
BfuAI ACCTGC 2 cut(s) 68, 551
BglII AGATCT 1 cut(s) 586
Bme1390I CCNGG 3 cut(s) 170, 771, 963
Bme18I GGWCC 2 cut(s) 690, 852
BmeT110I CYCGRG 1 cut(s) 590
BmgT120I GGNCC 3 cut(s) 167, 690, 852
BmiI GGNNCC 3 cut(s) 139, 765, 1129
BmrFI CCNGG 3 cut(s) 170, 771, 963
BmrI ACTGGG 1 cut(s) 716
BmsI GCATC 2 cut(s) 55, 741
BmuI ACTGGG 1 cut(s) 716
BpiI GAAGAC 1 cut(s) 719
BplI GAGNNNNNCTC 2 cut(s) 93, 125
Bpu10I CCTNAGC 1 cut(s) 880
BpuEI CTTGAG 1 cut(s) 790
BpuMI CCSGG 1 cut(s) 170
BsaBI GATNNNNATC 1 cut(s) 620
BsaHI GRCGYC 1 cut(s) 138
BsaI GGTCTC 1 cut(s) 559
BsaJI CCNNGG 3 cut(s) 755, 832, 962
BsaWI WCCGGW 1 cut(s) 854
Bsc4I CCNNNNNNNGG 2 cut(s) 175, 982
Bse1I ACTGG 3 cut(s) 335, 722, 787
Bse8I GATNNNNATC 1 cut(s) 620
BseAI TCCGGA 1 cut(s) 854
BseBI CCWGG 2 cut(s) 771, 963
BseDI CCNNGG 3 cut(s) 755, 832, 962
BseJI GATNNNNATC 1 cut(s) 620
BseLI CCNNNNNNNGG 2 cut(s) 175, 982
BseMII CTCAG 3 cut(s) 112, 570, 894
BseNI ACTGG 3 cut(s) 335, 722, 787
BseRI GAGGAG 2 cut(s) 333, 833
Bsh1236I CGCG 2 cut(s) 494, 626
BshFI GGCC 1 cut(s) 168
BshNI GGYRCC 2 cut(s) 137, 1127
BsiHKCI CYCGRG 1 cut(s) 590
BsiSI CCGG 3 cut(s) 170, 347, 855
BslFI GGGAC 1 cut(s) 620
BslI CCNNNNNNNGG 2 cut(s) 175, 982
BsmAI GTCTC 1 cut(s) 559
BsmFI GGGAC 1 cut(s) 620
BsnI GGCC 1 cut(s) 168
Bso31I GGTCTC 1 cut(s) 559
BsoBI CYCGRG 1 cut(s) 590
Bsp13I TCCGGA 1 cut(s) 854
Bsp143I GATC 4 cut(s) 210, 586, 763, 958
Bsp19I CCATGG 1 cut(s) 755
BspACI CCGC 2 cut(s) 494, 693
BspANI GGCC 1 cut(s) 168
BspCNI CTCAG 3 cut(s) 111, 569, 893
BspEI TCCGGA 1 cut(s) 854
BspFNI CGCG 2 cut(s) 494, 626
BspLI GGNNCC 3 cut(s) 139, 765, 1129
BspMI ACCTGC 2 cut(s) 68, 551
BspPI GGATC 4 cut(s) 218, 758, 771, 953
BspT107I GGYRCC 2 cut(s) 137, 1127
BspTNI GGTCTC 1 cut(s) 559
BsrI ACTGG 3 cut(s) 335, 722, 787
BssECI CCNNGG 3 cut(s) 755, 832, 962
BssMI GATC 4 cut(s) 210, 586, 763, 958
BssNI GRCGYC 1 cut(s) 138
BssT1I CCWWGG 2 cut(s) 755, 832
Bst2UI CCWGG 2 cut(s) 771, 963
Bst4CI ACNGT 5 cut(s) 62, 440, 580, 909, 1138
Bst6I CTCTTC 2 cut(s) 380, 512
BstACI GRCGYC 1 cut(s) 138
BstDEI CTNAG 4 cut(s) 98, 122, 556, 880
BstDSI CCRYGG 1 cut(s) 755
BstFNI CGCG 2 cut(s) 494, 626
BstH2I RGCGCY 4 cut(s) 141, 148, 612, 813
BstHHI GCGC 5 cut(s) 140, 147, 611, 703, 812
BstKTI GATC 4 cut(s) 213, 589, 766, 961
BstMAI GTCTC 1 cut(s) 559
BstMBI GATC 4 cut(s) 210, 586, 763, 958
BstNI CCWGG 2 cut(s) 771, 963
BstSCI CCNGG 3 cut(s) 168, 769, 961
BstUI CGCG 2 cut(s) 494, 626
BstV2I GAAGAC 1 cut(s) 719
BstX2I RGATCY 3 cut(s) 586, 763, 958
BstYI RGATCY 3 cut(s) 586, 763, 958
BsuRI GGCC 1 cut(s) 168
BtgI CCRYGG 1 cut(s) 755
BtgZI GCGATG 1 cut(s) 194
BtsIMutI CAGTG 3 cut(s) 67, 445, 794
BveI ACCTGC 2 cut(s) 68, 551
CfoI GCGC 5 cut(s) 140, 147, 611, 703, 812
Cfr13I GGNCC 3 cut(s) 167, 690, 852
CseI GACGC 1 cut(s) 328
Csp6I GTAC 1 cut(s) 1128
CviAII CATG 5 cut(s) 196, 294, 394, 756, 893
CviQI GTAC 1 cut(s) 1128
DdeI CTNAG 4 cut(s) 98, 122, 556, 880
DinI GGCGCC 1 cut(s) 139
DpnI GATC 4 cut(s) 212, 588, 765, 960
DpnII GATC 4 cut(s) 210, 586, 763, 958
DraIII CACNNNGTG 1 cut(s) 286
Eam1104I CTCTTC 2 cut(s) 380, 512
EarI CTCTTC 2 cut(s) 380, 512
Eco130I CCWWGG 2 cut(s) 755, 832
Eco31I GGTCTC 1 cut(s) 559
Eco32I GATATC 1 cut(s) 796
Eco47I GGWCC 2 cut(s) 690, 852
Eco57I CTGAAG 2 cut(s) 114, 493
Eco88I CYCGRG 1 cut(s) 590
EcoRII CCWGG 2 cut(s) 769, 961
EcoRV GATATC 1 cut(s) 796
EcoT14I CCWWGG 2 cut(s) 755, 832
EgeI GGCGCC 1 cut(s) 139
EheI GGCGCC 1 cut(s) 139
ErhI CCWWGG 2 cut(s) 755, 832
FaeI CATG 5 cut(s) 199, 297, 397, 759, 896
FalI AAGNNNNNCTT 2 cut(s) 1049, 1081
FaqI GGGAC 1 cut(s) 620
FatI CATG 5 cut(s) 195, 293, 393, 755, 892
FspBI CTAG 5 cut(s) 305, 351, 545, 888, 932
GlaI GCGC 5 cut(s) 139, 146, 610, 702, 811
HaeII RGCGCY 4 cut(s) 141, 148, 612, 813
HaeIII GGCC 1 cut(s) 168
HapII CCGG 3 cut(s) 170, 347, 855
HgaI GACGC 1 cut(s) 328
HhaI GCGC 5 cut(s) 140, 147, 611, 703, 812
Hin1I GRCGYC 1 cut(s) 138
Hin1II CATG 5 cut(s) 199, 297, 397, 759, 896
Hin6I GCGC 5 cut(s) 138, 145, 609, 701, 810
HinP1I GCGC 5 cut(s) 138, 145, 609, 701, 810
HinfI GANTC 3 cut(s) 14, 277, 1112
HpaII CCGG 3 cut(s) 170, 347, 855
Hpy166II GTNNAC 2 cut(s) 649, 1015
Hpy188I TCNGA 7 cut(s) 101, 133, 219, 512, 750, 958, 1117
Hpy188III TCNNGA 3 cut(s) 590, 855, 970
Hpy8I GTNNAC 2 cut(s) 649, 1015
HpyAV CCTTC 2 cut(s) 128, 196
HpyCH4III ACNGT 5 cut(s) 62, 440, 580, 909, 1138
HpyCH4V TGCA 3 cut(s) 68, 620, 673
HpyF3I CTNAG 4 cut(s) 98, 122, 556, 880
Hsp92I GRCGYC 1 cut(s) 138
Hsp92II CATG 5 cut(s) 199, 297, 397, 759, 896
HspAI GCGC 5 cut(s) 138, 145, 609, 701, 810
KasI GGCGCC 1 cut(s) 137
Kpn2I TCCGGA 1 cut(s) 854
KpnI GGTACC 1 cut(s) 1131
Kzo9I GATC 4 cut(s) 210, 586, 763, 958
LmnI GCTCC 1 cut(s) 857
LweI GCATC 2 cut(s) 55, 741
MaeI CTAG 5 cut(s) 305, 351, 545, 888, 932
MaeIII GTNAC 8 cut(s) 286, 472, 527, 580, 724, 800, 988, 1051
MalI GATC 4 cut(s) 212, 588, 765, 960
MboI GATC 4 cut(s) 210, 586, 763, 958
MboII GAAGA 6 cut(s) 104, 323, 367, 499, 724, 727
MfeI CAATTG 1 cut(s) 685
MflI RGATCY 3 cut(s) 586, 763, 958
MluCI AATT 5 cut(s) 190, 327, 465, 598, 685
Mly113I GGCGCC 1 cut(s) 138
MlyI GAGTC 1 cut(s) 1121
MmeI TCCRAC 2 cut(s) 226, 741
MnlI CCTC 6 cut(s) 95, 354, 854, 889, 1007, 1117
MroI TCCGGA 1 cut(s) 854
MseI TTAA 4 cut(s) 252, 315, 1076, 1092
MslI CAYNNNNRTG 1 cut(s) 438
MspI CCGG 3 cut(s) 170, 347, 855
MspR9I CCNGG 3 cut(s) 170, 771, 963
MunI CAATTG 1 cut(s) 685
MvaI CCWGG 2 cut(s) 771, 963
MvnI CGCG 2 cut(s) 494, 626
NarI GGCGCC 1 cut(s) 138
NciI CCSGG 1 cut(s) 170
NcoI CCATGG 1 cut(s) 755
NdeII GATC 4 cut(s) 210, 586, 763, 958
NlaIII CATG 5 cut(s) 199, 297, 397, 759, 896
NlaIV GGNNCC 3 cut(s) 139, 765, 1129
NmuCI GTSAC 4 cut(s) 286, 472, 724, 1051
OliI CACNNNNGTG 1 cut(s) 438
PaeR7I CTCGAG 1 cut(s) 590
PaqCI CACCTGC 1 cut(s) 551
PfeI GAWTC 2 cut(s) 14, 277
PleI GAGTC 1 cut(s) 1120
PluTI GGCGCC 1 cut(s) 141
PpsI GAGTC 1 cut(s) 1120
Psp6I CCWGG 2 cut(s) 769, 961
PspGI CCWGG 2 cut(s) 769, 961
PspN4I GGNNCC 3 cut(s) 139, 765, 1129
PspPI GGNCC 3 cut(s) 167, 690, 852
PsuI RGATCY 3 cut(s) 586, 763, 958
RsaI GTAC 1 cut(s) 1129
RsaNI GTAC 1 cut(s) 1128
RseI CAYNNNNRTG 1 cut(s) 438
SaqAI TTAA 4 cut(s) 252, 315, 1076, 1092
Sau3AI GATC 4 cut(s) 210, 586, 763, 958
Sau96I GGNCC 3 cut(s) 167, 690, 852
SchI GAGTC 1 cut(s) 1121
ScrFI CCNGG 3 cut(s) 170, 771, 963
SfaNI GCATC 2 cut(s) 55, 741
SfoI GGCGCC 1 cut(s) 139
Sfr274I CTCGAG 1 cut(s) 590
SinI GGWCC 2 cut(s) 690, 852
SlaI CTCGAG 1 cut(s) 590
SmiMI CAYNNNNRTG 1 cut(s) 438
SmlI CTYRAG 2 cut(s) 590, 805
SmoI CTYRAG 2 cut(s) 590, 805
Sse9I AATT 5 cut(s) 190, 327, 465, 598, 685
SsiI CCGC 2 cut(s) 494, 693
SspDI GGCGCC 1 cut(s) 137
SspMI CTAG 5 cut(s) 305, 351, 545, 888, 932
StyD4I CCNGG 3 cut(s) 168, 769, 961
StyI CCWWGG 2 cut(s) 755, 832
TaaI ACNGT 5 cut(s) 62, 440, 580, 909, 1138
TaqI TCGA 1 cut(s) 591
TasI AATT 5 cut(s) 190, 327, 465, 598, 685
TfiI GAWTC 2 cut(s) 14, 277
Tru1I TTAA 4 cut(s) 252, 315, 1076, 1092
Tru9I TTAA 4 cut(s) 252, 315, 1076, 1092
TscAI CASTG 3 cut(s) 67, 445, 794
TseFI GTSAC 4 cut(s) 286, 472, 724, 1051
Tsp45I GTSAC 4 cut(s) 286, 472, 724, 1051
TspDTI ATGAA 4 cut(s) 6, 45, 184, 490
TspGWI ACGGA 1 cut(s) 1136
TspRI CASTG 3 cut(s) 67, 445, 794
VpaK11BI GGWCC 2 cut(s) 690, 852
XcmI CCANNNNNNNNNTGG 1 cut(s) 899
XhoI CTCGAG 1 cut(s) 590
XspI CTAG 5 cut(s) 305, 351, 545, 888, 932
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.