MD11G1035900.v1.1

membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0010952
Physical Location & Seq
Reverse (-)
3134128 .. 3137080
2953 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1035900.v1.1.491

Sequence Viewer

Length: 1173 bp
ATGATCCCACCATTTGGTATGGGGCTGTGGGATTCATTGGTGGGGAACGAAGTTAAGAAGTTCATCAAACGAAAAGATAGTGATGCAGGAGAAGCAGGTCGAGCGCTTGAAGAATTCCGAGGCTCTATCTACAATGACCTCCGAACATCAGAAGGCGCCAAGCGCCAACAGCAGCGTTTTTGTGGTCCGGTTGTGGCTATGTCCTTCAATTTCATAGTCTCTGTGGGGATCATCCTTGCAAACAAACTAATAATGGGGAGAGTTGGATTTAAGTTCCCAATCTTTCTCACATTGATCCATTATGTGACAGCATGGCTGCTTCTCGCCATTTTCAAGTCGCTCTCAATTCTTCCTGTTGCTCCTCCTTCGAGAACCACTCCTTTCACTTCTCTCTTCTCCTTAGGCGCGGTCATGGCTTTTGCATCCGGTCTTGCAAACACTAGCCTAAAACATAACAGTGTTGGCTTCTACCAGATGGCTAAAATTGCTGTCACTCCTACCATTGTTTTCACGGAGTTCATTCTCTTCAGAAAAACCATTTCTTTTAACAAGGTTTTGGCTCTAGCTGTAGTCTCGGCAGGTGTGGCAGTAGCAACTGTAACAGATTTAGAGTTCAATTTATTTGGTGCTTTGATTGCAATTGCATGGATAGTTCCAAGTGCTGTAAACAAAATCTTATGGTCTAATCTTCAGCAGCAAGGCAATTGGACCGCGCTCGCGCTGATGTGGAAGACAACCCCGGTCACGATATTCTTCTTGCTGGCTCTGATGCCTTGGTTGGATCCACCAGGCGTCCTACTTTTCAAGTGGGATTTCAGTAACTCAACCGCTATTCTTACATCAGCAGTCCTTGGTTTTCTCCTCCAATGGTCCGGAGCTTTGGCACTCGGGGCAACCTCAGCAACTTCCCACGTTGTTCTAGGGCAGTTCAAGACTTGTGTTATACTACTAGGGGGCTACCTTATATTTAACTCGGATCCGGGCTTTGTGAGCATTTGCGGGGCTGTCGCAGCTCTCTGCGGAATGTCTGTTTACACGTCGCTAAACCTGAAAAAGCCGCAAGACAGCACGAGCAAACAGCTCCCAAAGCAAAGCTTACCGACGTCAAAACCCAAAGCCACTGACGGAGAAACTGTAGAGACAGAAGTAGAAGATACTGCAACTGTTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPT

Protein Analysis

391

Amino Acids

42.16

Weight (kDa)

9.37

Isoelectric Point (pI)

34.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPT PF03151 70 - 345 1.3e-13 Triose-phosphate Transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010952)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55950 AT5G55950 AT5G55950 AT5G55950
fragaria_vesca FvH4_3g42500 FvH4_3g42500
malus_domestica MD11G1035900.v1.1
prunus_persica Prupe.6G026800_v2.0.a1 Prupe.6G026800_v2.0.a1
pyrus_communis pycom11g02970
rosa_chinensis RchiOBHm_Chr5g0076161
rosa_laevigata RLG00000036606
rosa_multiflora Rmu_ssc0000123.1_g000002
rosa_roxburghii Rroxscaffold_1G00005650
rosa_rugosa Rorug05G0445200
rosa_samantha Rh5AG501300 Rh5BG522800 Rh5CG546600 Rh5DG535200
rosa_wichuraiana Rw5G046530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 569
AatII GACGTC 1 cut(s) 1106
Acc36I ACCTGC 2 cut(s) 86, 569
AccB1I GGYRCC 1 cut(s) 155
AccB7I CCANNNNNTGG 1 cut(s) 14
AccII CGCG 3 cut(s) 407, 713, 719
AccIII TCCGGA 1 cut(s) 872
AciI CCGC 6 cut(s) 407, 711, 828, 999, 1020, 1058
AclWI GGATC 6 cut(s) 236, 289, 776, 789, 971, 984
AcsI RAATTY 1 cut(s) 113
AcuI CTGAAG 2 cut(s) 511, 674
AcyI GRCGYC 3 cut(s) 156, 792, 1103
AfeI AGCGCT 1 cut(s) 105
AfiI CCNNNNNNNGG 1 cut(s) 14
AflIII ACRYGT 1 cut(s) 1035
AgsI TTSAA 6 cut(s) 110, 208, 334, 616, 805, 931
AjiI CACGTC 1 cut(s) 1038
AjnI CCWGG 1 cut(s) 787
AluBI AGCT 5 cut(s) 566, 878, 1013, 1081, 1095
AluI AGCT 5 cut(s) 566, 878, 1013, 1081, 1095
Alw26I GTCTC 3 cut(s) 223, 577, 1133
AlwI GGATC 6 cut(s) 236, 289, 776, 789, 971, 984
Ama87I CYCGRG 1 cut(s) 887
Aor13HI TCCGGA 1 cut(s) 872
Aor51HI AGCGCT 1 cut(s) 105
ApeKI GCWGC 4 cut(s) 172, 316, 694, 1010
ApoI RAATTY 1 cut(s) 113
AspLEI GCGC 6 cut(s) 106, 158, 165, 407, 715, 721
AspS9I GGNCC 3 cut(s) 185, 708, 870
AsuC2I CCSGG 2 cut(s) 740, 981
AvaI CYCGRG 1 cut(s) 887
AvaII GGWCC 3 cut(s) 185, 708, 870
AxyI CCTNAGG 1 cut(s) 400
BamHI GGATCC 2 cut(s) 781, 976
BanI GGYRCC 1 cut(s) 155
BauI CACGAG 1 cut(s) 1069
BbsI GAAGAC 1 cut(s) 737
BbvCI CCTCAGC 1 cut(s) 898
BbvI GCAGC 4 cut(s) 184, 303, 706, 1022
BccI CCATC 1 cut(s) 469
BciT130I CCWGG 1 cut(s) 789
BcnI CCSGG 2 cut(s) 740, 981
BcoDI GTCTC 3 cut(s) 223, 577, 1133
BfaI CTAG 4 cut(s) 441, 563, 920, 950
BfmI CTRYAG 2 cut(s) 567, 1134
BfoI RGCGCY 3 cut(s) 107, 159, 166
BfuAI ACCTGC 2 cut(s) 86, 569
BisI GCNGC 5 cut(s) 173, 317, 695, 1011, 1058
BlsI GCNGC 5 cut(s) 174, 318, 696, 1012, 1059
Bme1390I CCNGG 3 cut(s) 740, 789, 981
Bme18I GGWCC 3 cut(s) 185, 708, 870
BmeT110I CYCGRG 1 cut(s) 887
BmgBI CACGTC 1 cut(s) 1038
BmgT120I GGNCC 3 cut(s) 185, 708, 870
BmiI GGNNCC 3 cut(s) 157, 783, 978
BmrFI CCNGG 3 cut(s) 740, 789, 981
BmsI GCATC 3 cut(s) 73, 431, 759
BpiI GAAGAC 1 cut(s) 737
BplI GAGNNNNNCTC 2 cut(s) 361, 393
Bpu10I CCTNAGC 1 cut(s) 898
BpuMI CCSGG 2 cut(s) 740, 981
BsaHI GRCGYC 3 cut(s) 156, 792, 1103
BsaJI CCNNGG 4 cut(s) 118, 738, 773, 850
BsaWI WCCGGW 3 cut(s) 187, 425, 872
BsaXI ACNNNNNCTCC 2 cut(s) 1119, 1149
Bsc4I CCNNNNNNNGG 1 cut(s) 14
Bse21I CCTNAGG 1 cut(s) 400
BseAI TCCGGA 1 cut(s) 872
BseBI CCWGG 1 cut(s) 789
BseDI CCNNGG 4 cut(s) 118, 738, 773, 850
BseGI GGATG 2 cut(s) 231, 422
BseLI CCNNNNNNNGG 1 cut(s) 14
BseMII CTCAG 1 cut(s) 912
BseRI GAGGAG 2 cut(s) 351, 851
BseXI GCAGC 4 cut(s) 184, 303, 706, 1022
Bsh1236I CGCG 3 cut(s) 407, 713, 719
BshNI GGYRCC 1 cut(s) 155
BsiHKCI CYCGRG 1 cut(s) 887
BsiSI CCGG 5 cut(s) 188, 426, 740, 873, 980
BslI CCNNNNNNNGG 1 cut(s) 14
BsmAI GTCTC 3 cut(s) 223, 577, 1133
BsoBI CYCGRG 1 cut(s) 887
Bsp13I TCCGGA 1 cut(s) 872
Bsp143I GATC 5 cut(s) 3, 228, 294, 781, 976
BspACI CCGC 6 cut(s) 407, 711, 828, 999, 1020, 1058
BspCNI CTCAG 1 cut(s) 911
BspEI TCCGGA 1 cut(s) 872
BspFNI CGCG 3 cut(s) 407, 713, 719
BspLI GGNNCC 3 cut(s) 157, 783, 978
BspMI ACCTGC 2 cut(s) 86, 569
BspPI GGATC 6 cut(s) 236, 289, 776, 789, 971, 984
BspT107I GGYRCC 1 cut(s) 155
BssECI CCNNGG 4 cut(s) 118, 738, 773, 850
BssMI GATC 5 cut(s) 3, 228, 294, 781, 976
BssNI GRCGYC 3 cut(s) 156, 792, 1103
BssSI CACGAG 1 cut(s) 1069
BssT1I CCWWGG 2 cut(s) 773, 850
Bst2BI CACGAG 1 cut(s) 1069
Bst2UI CCWGG 1 cut(s) 789
Bst4CI ACNGT 4 cut(s) 458, 598, 1135, 1165
Bst6I CTCTTC 2 cut(s) 398, 530
BstACI GRCGYC 3 cut(s) 156, 792, 1103
BstC8I GCNNGC 2 cut(s) 717, 762
BstDEI CTNAG 2 cut(s) 400, 898
BstF5I GGATG 2 cut(s) 231, 422
BstFNI CGCG 3 cut(s) 407, 713, 719
BstH2I RGCGCY 3 cut(s) 107, 159, 166
BstHHI GCGC 6 cut(s) 106, 158, 165, 407, 715, 721
BstKTI GATC 5 cut(s) 6, 231, 297, 784, 979
BstMAI GTCTC 3 cut(s) 223, 577, 1133
BstMBI GATC 5 cut(s) 3, 228, 294, 781, 976
BstNI CCWGG 1 cut(s) 789
BstSCI CCNGG 3 cut(s) 738, 787, 979
BstSFI CTRYAG 2 cut(s) 567, 1134
BstUI CGCG 3 cut(s) 407, 713, 719
BstV1I GCAGC 4 cut(s) 184, 303, 706, 1022
BstV2I GAAGAC 1 cut(s) 737
BstX2I RGATCY 2 cut(s) 781, 976
BstYI RGATCY 2 cut(s) 781, 976
Bsu36I CCTNAGG 1 cut(s) 400
BtrI CACGTC 1 cut(s) 1038
BtsCI GGATG 2 cut(s) 231, 422
BtsIMutI CAGTG 2 cut(s) 463, 1119
BveI ACCTGC 2 cut(s) 86, 569
Cac8I GCNNGC 2 cut(s) 717, 762
CfoI GCGC 6 cut(s) 106, 158, 165, 407, 715, 721
Cfr13I GGNCC 3 cut(s) 185, 708, 870
CseI GACGC 1 cut(s) 781
CviAII CATG 3 cut(s) 312, 412, 645
DdeI CTNAG 2 cut(s) 400, 898
DinI GGCGCC 1 cut(s) 157
DpnI GATC 5 cut(s) 5, 230, 296, 783, 978
DpnII GATC 5 cut(s) 3, 228, 294, 781, 976
Eam1104I CTCTTC 2 cut(s) 398, 530
EarI CTCTTC 2 cut(s) 398, 530
Eco130I CCWWGG 2 cut(s) 773, 850
Eco47I GGWCC 3 cut(s) 185, 708, 870
Eco47III AGCGCT 1 cut(s) 105
Eco57I CTGAAG 2 cut(s) 511, 674
Eco81I CCTNAGG 1 cut(s) 400
Eco88I CYCGRG 1 cut(s) 887
EcoRI GAATTC 1 cut(s) 113
EcoRII CCWGG 1 cut(s) 787
EcoT14I CCWWGG 2 cut(s) 773, 850
EgeI GGCGCC 1 cut(s) 157
EheI GGCGCC 1 cut(s) 157
ErhI CCWWGG 2 cut(s) 773, 850
FaeI CATG 3 cut(s) 315, 415, 648
FalI AAGNNNNNCTT 2 cut(s) 1079, 1111
FatI CATG 3 cut(s) 311, 411, 644
FauI CCCGC 1 cut(s) 992
Fnu4HI GCNGC 5 cut(s) 173, 317, 695, 1011, 1058
FokI GGATG 2 cut(s) 218, 409
Fsp4HI GCNGC 5 cut(s) 173, 317, 695, 1011, 1058
FspBI CTAG 4 cut(s) 441, 563, 920, 950
GlaI GCGC 6 cut(s) 105, 157, 164, 406, 714, 720
GluI GCNGC 5 cut(s) 173, 317, 695, 1011, 1058
HaeII RGCGCY 3 cut(s) 107, 159, 166
HapII CCGG 5 cut(s) 188, 426, 740, 873, 980
HgaI GACGC 1 cut(s) 781
HhaI GCGC 6 cut(s) 106, 158, 165, 407, 715, 721
Hin1I GRCGYC 3 cut(s) 156, 792, 1103
Hin1II CATG 3 cut(s) 315, 415, 648
Hin6I GCGC 6 cut(s) 104, 156, 163, 405, 713, 719
HinP1I GCGC 6 cut(s) 104, 156, 163, 405, 713, 719
HindIII AAGCTT 1 cut(s) 1093
HinfI GANTC 1 cut(s) 32
HpaII CCGG 5 cut(s) 188, 426, 740, 873, 980
Hpy166II GTNNAC 2 cut(s) 667, 1033
Hpy188I TCNGA 7 cut(s) 119, 143, 151, 530, 768, 976, 1172
Hpy188III TCNNGA 4 cut(s) 369, 745, 873, 931
Hpy8I GTNNAC 2 cut(s) 667, 1033
Hpy99I CGWCG 2 cut(s) 1042, 1105
HpyAV CCTTC 3 cut(s) 146, 214, 375
HpyCH4III ACNGT 4 cut(s) 458, 598, 1135, 1165
HpyCH4IV ACGT 3 cut(s) 912, 1037, 1103
HpyCH4V TGCA 7 cut(s) 86, 239, 422, 434, 638, 644, 1160
HpyF3I CTNAG 2 cut(s) 400, 898
HpySE526I ACGT 3 cut(s) 912, 1037, 1103
Hsp92I GRCGYC 3 cut(s) 156, 792, 1103
Hsp92II CATG 3 cut(s) 315, 415, 648
HspAI GCGC 6 cut(s) 104, 156, 163, 405, 713, 719
KasI GGCGCC 1 cut(s) 155
Kpn2I TCCGGA 1 cut(s) 872
Kzo9I GATC 5 cut(s) 3, 228, 294, 781, 976
LmnI GCTCC 3 cut(s) 364, 875, 1086
Lsp1109I GCAGC 4 cut(s) 184, 303, 706, 1022
LweI GCATC 3 cut(s) 73, 431, 759
MaeI CTAG 4 cut(s) 441, 563, 920, 950
MaeII ACGT 3 cut(s) 912, 1037, 1103
MaeIII GTNAC 5 cut(s) 304, 490, 598, 742, 818
MalI GATC 5 cut(s) 5, 230, 296, 783, 978
MboI GATC 5 cut(s) 3, 228, 294, 781, 976
MboII GAAGA 8 cut(s) 122, 341, 385, 517, 680, 742, 745, 1163
MfeI CAATTG 2 cut(s) 639, 703
MflI RGATCY 2 cut(s) 781, 976
MluCI AATT 7 cut(s) 113, 208, 345, 483, 616, 639, 703
Mly113I GGCGCC 1 cut(s) 156
MmeI TCCRAC 2 cut(s) 244, 759
MnlI CCTC 5 cut(s) 113, 149, 372, 872, 907
MroI TCCGGA 1 cut(s) 872
MseI TTAA 4 cut(s) 54, 270, 546, 969
MslI CAYNNNNRTG 1 cut(s) 456
MspI CCGG 5 cut(s) 188, 426, 740, 873, 980
MspR9I CCNGG 3 cut(s) 740, 789, 981
MunI CAATTG 2 cut(s) 639, 703
MvaI CCWGG 1 cut(s) 789
MvnI CGCG 3 cut(s) 407, 713, 719
NarI GGCGCC 1 cut(s) 156
NciI CCSGG 2 cut(s) 740, 981
NdeII GATC 5 cut(s) 3, 228, 294, 781, 976
NlaIII CATG 3 cut(s) 315, 415, 648
NlaIV GGNNCC 3 cut(s) 157, 783, 978
NmeAIII GCCGAG 1 cut(s) 554
NmuCI GTSAC 3 cut(s) 304, 490, 742
PaqCI CACCTGC 1 cut(s) 569
PfeI GAWTC 1 cut(s) 32
PflMI CCANNNNNTGG 1 cut(s) 14
PkrI GCNGC 5 cut(s) 174, 318, 696, 1012, 1059
PluTI GGCGCC 1 cut(s) 159
Psp6I CCWGG 1 cut(s) 787
PspGI CCWGG 1 cut(s) 787
PspN4I GGNNCC 3 cut(s) 157, 783, 978
PspPI GGNCC 3 cut(s) 185, 708, 870
PsuI RGATCY 2 cut(s) 781, 976
RseI CAYNNNNRTG 1 cut(s) 456
SaqAI TTAA 4 cut(s) 54, 270, 546, 969
SatI GCNGC 5 cut(s) 173, 317, 695, 1011, 1058
Sau3AI GATC 5 cut(s) 3, 228, 294, 781, 976
Sau96I GGNCC 3 cut(s) 185, 708, 870
ScrFI CCNGG 3 cut(s) 740, 789, 981
SfaNI GCATC 3 cut(s) 73, 431, 759
SfcI CTRYAG 2 cut(s) 567, 1134
SfoI GGCGCC 1 cut(s) 157
SinI GGWCC 3 cut(s) 185, 708, 870
SmiMI CAYNNNNRTG 1 cut(s) 456
Sse9I AATT 7 cut(s) 113, 208, 345, 483, 616, 639, 703
SsiI CCGC 6 cut(s) 407, 711, 828, 999, 1020, 1058
SspDI GGCGCC 1 cut(s) 155
SspMI CTAG 4 cut(s) 441, 563, 920, 950
StyD4I CCNGG 3 cut(s) 738, 787, 979
StyI CCWWGG 2 cut(s) 773, 850
TaaI ACNGT 4 cut(s) 458, 598, 1135, 1165
TaiI ACGT 3 cut(s) 915, 1040, 1106
TaqI TCGA 2 cut(s) 100, 368
TasI AATT 7 cut(s) 113, 208, 345, 483, 616, 639, 703
TauI GCSGC 1 cut(s) 1060
TfiI GAWTC 1 cut(s) 32
Tru1I TTAA 4 cut(s) 54, 270, 546, 969
Tru9I TTAA 4 cut(s) 54, 270, 546, 969
TscAI CASTG 2 cut(s) 463, 1126
TseFI GTSAC 3 cut(s) 304, 490, 742
TseI GCWGC 4 cut(s) 172, 316, 694, 1010
Tsp45I GTSAC 3 cut(s) 304, 490, 742
TspDTI ATGAA 4 cut(s) 24, 52, 202, 508
TspGWI ACGGA 2 cut(s) 527, 1140
TspRI CASTG 2 cut(s) 463, 1126
Van91I CCANNNNNTGG 1 cut(s) 14
VpaK11BI GGWCC 3 cut(s) 185, 708, 870
XapI RAATTY 1 cut(s) 113
XspI CTAG 4 cut(s) 441, 563, 920, 950
ZraI GACGTC 1 cut(s) 1104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.