Rroxscaffold_1G00005650

membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0010952
Physical Location & Seq
Reverse (-)
7643466 .. 7646324
2859 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00005650.1

Sequence Viewer

Length: 1185 bp
ATGATCCCATATATATGCCTCTGCCATTGTCAGATTGGAATGGCTTTGCTTGATTCATTGTTGGGGAATGAAGCCAAGAGATTTGTGAAAAGGAAAGACAGTGATGCAGGAGAAGCAGGTAAAGCACTTGAAGAACTCAGAGGTTCTCTCTACAATGAGCTTAGAACTTCAGAAGGCGCCAAGCGCCAACAGCAACGATTTTGTGGCCCGGTGGTGGCGATGACCTTCAATTTCATGGTGTCTGTGGGGATCATTCTGGCAAATAAACTTGTAATGGGGAGAGTTGGATTTAAGTTTCCAATCTTTCTCACATTGATTCATTATGTGACAGCATGGTTGCTTCTAGCCATTTTTAAGACACTCTCAATTCTTCCGGTTTCTCCTCCGGCTAGAACTACTCCTTTTACTTCTCTCTTCTCTTTAGGTGCTGTCATGGCTTTTGCTTCTGGTCTTGCCAATACAAGCCTAAAACACAACAGTGTTGGTTTCTACCAGATGGCTAAAATTGCTGTCACTCCTACCATTGTTCTCGCGGAGTTCATTCTCTTCAGAAAAACCATTTCTTGTAACAAGGTTTTAGCTCTAGCTGTGGTCTCAGCAGGTGTGGCAGTAGCAACAGTAACAGATCTAGAGTTCAATTTGTTTGGCGCTATGATTGCAATCGCGTGGATAGTCCCAAGTGCTGTAAACAAAATCCTATGGTCTAATCTGCAACAGCAAGGCAATTGGACCGCCCTTGCGCTGATGTGGAAGACTACCCCAGTCACTGTATTCTTCTTATTAGCTCTGATGCCATGGTTGGATCCACCAGGAGTTCTACTATTCCAGTGGGATATCAATAACTCAAGCGCCATTCTTATATCAGCAATCCTTGGTTTTCTCCTCCAATGGTCCGGAGCATTGGCACTTGGGGCAACCTCAGCAACTAGCCATGTTGTTCTTGGACAGTTCAAAACTTGTGTGATATTACTAGGGGGCTATTTTCTTTTCAAATCAGATCCAGGGTTCGTGAGCCTTTGTGGGGCTGTTACAGCCCTCTGTGGAATGTCTGTTTACACATCACTAAACCTGAAAAAGCCACAAGACAATGTGAACAAGCAAACCTTACCGACATTAAAACCCAAAACCATTGACCAAGAAAATGTAGTAGAGTCAGAAGCTAAGGATACCCCAACTGTTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPT

Protein Analysis

394

Amino Acids

42.73

Weight (kDa)

9.22

Isoelectric Point (pI)

34.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPT PF03151 75 - 352 2.1e-13 Triose-phosphate Transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010952)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55950 AT5G55950 AT5G55950 AT5G55950
fragaria_vesca FvH4_3g42500 FvH4_3g42500
malus_domestica MD11G1035900.v1.1
prunus_persica Prupe.6G026800_v2.0.a1 Prupe.6G026800_v2.0.a1
pyrus_communis pycom11g02970
rosa_chinensis RchiOBHm_Chr5g0076161
rosa_laevigata RLG00000036606
rosa_multiflora Rmu_ssc0000123.1_g000002
rosa_roxburghii Rroxscaffold_1G00005650
rosa_rugosa Rorug05G0445200
rosa_samantha Rh5AG501300 Rh5BG522800 Rh5CG546600 Rh5DG535200
rosa_wichuraiana Rw5G046530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 590
Acc36I ACCTGC 2 cut(s) 107, 590
AccB1I GGYRCC 1 cut(s) 176
AccII CGCG 2 cut(s) 533, 665
AccIII TCCGGA 1 cut(s) 893
AciI CCGC 2 cut(s) 533, 732
AclWI GGATC 4 cut(s) 257, 797, 810, 992
AcuI CTGAAG 2 cut(s) 153, 532
AcyI GRCGYC 1 cut(s) 177
AfiI CCNNNNNNNGG 2 cut(s) 214, 1021
AgsI TTSAA 5 cut(s) 131, 229, 637, 952, 991
AjnI CCWGG 2 cut(s) 808, 1000
AleI CACNNNNGTG 1 cut(s) 477
AluBI AGCT 5 cut(s) 160, 581, 587, 785, 1160
AluI AGCT 5 cut(s) 160, 581, 587, 785, 1160
Alw26I GTCTC 1 cut(s) 598
AlwI GGATC 4 cut(s) 257, 797, 810, 992
AlwNI CAGNNNCTG 1 cut(s) 767
Aor13HI TCCGGA 1 cut(s) 893
AoxI GGCC 1 cut(s) 205
AspLEI GCGC 5 cut(s) 179, 186, 650, 742, 851
AspS9I GGNCC 3 cut(s) 206, 729, 891
AsuC2I CCSGG 1 cut(s) 209
AvaII GGWCC 2 cut(s) 729, 891
BamHI GGATCC 1 cut(s) 802
BanI GGYRCC 1 cut(s) 176
BbsI GAAGAC 1 cut(s) 758
BbvCI CCTCAGC 1 cut(s) 919
BccI CCATC 1 cut(s) 490
BciT130I CCWGG 2 cut(s) 810, 1002
BciVI GTATCC 1 cut(s) 1159
BcnI CCSGG 1 cut(s) 209
BcoDI GTCTC 1 cut(s) 598
BfaI CTAG 6 cut(s) 344, 390, 584, 629, 927, 971
BfoI RGCGCY 4 cut(s) 180, 187, 651, 852
BfuAI ACCTGC 2 cut(s) 107, 590
BfuI GTATCC 1 cut(s) 1159
BglII AGATCT 1 cut(s) 625
Bme1390I CCNGG 3 cut(s) 209, 810, 1002
Bme18I GGWCC 2 cut(s) 729, 891
BmgT120I GGNCC 3 cut(s) 206, 729, 891
BmiI GGNNCC 2 cut(s) 178, 804
BmrFI CCNGG 3 cut(s) 209, 810, 1002
BmrI ACTGGG 1 cut(s) 755
BmsI GCATC 2 cut(s) 94, 780
BmuI ACTGGG 1 cut(s) 755
BpiI GAAGAC 1 cut(s) 758
BplI GAGNNNNNCTC 2 cut(s) 132, 164
Bpu10I CCTNAGC 2 cut(s) 919, 1161
BpuEI CTTGAG 1 cut(s) 829
BpuMI CCSGG 1 cut(s) 209
BsaBI GATNNNNATC 1 cut(s) 659
BsaHI GRCGYC 1 cut(s) 177
BsaI GGTCTC 1 cut(s) 598
BsaJI CCNNGG 3 cut(s) 794, 871, 1001
BsaWI WCCGGW 2 cut(s) 373, 893
Bsc4I CCNNNNNNNGG 2 cut(s) 214, 1021
Bse1I ACTGG 2 cut(s) 761, 826
Bse8I GATNNNNATC 1 cut(s) 659
BseAI TCCGGA 1 cut(s) 893
BseBI CCWGG 2 cut(s) 810, 1002
BseDI CCNNGG 3 cut(s) 794, 871, 1001
BseJI GATNNNNATC 1 cut(s) 659
BseLI CCNNNNNNNGG 2 cut(s) 214, 1021
BseMII CTCAG 3 cut(s) 151, 609, 933
BseNI ACTGG 2 cut(s) 761, 826
BseRI GAGGAG 2 cut(s) 372, 872
Bsh1236I CGCG 2 cut(s) 533, 665
BshFI GGCC 1 cut(s) 207
BshNI GGYRCC 1 cut(s) 176
BsiSI CCGG 4 cut(s) 209, 374, 386, 894
BslFI GGGAC 1 cut(s) 659
BslI CCNNNNNNNGG 2 cut(s) 214, 1021
BsmAI GTCTC 1 cut(s) 598
BsmFI GGGAC 1 cut(s) 659
BsnI GGCC 1 cut(s) 207
Bso31I GGTCTC 1 cut(s) 598
Bsp13I TCCGGA 1 cut(s) 893
Bsp143I GATC 5 cut(s) 3, 249, 625, 802, 997
Bsp19I CCATGG 1 cut(s) 794
BspACI CCGC 2 cut(s) 533, 732
BspANI GGCC 1 cut(s) 207
BspCNI CTCAG 3 cut(s) 150, 608, 932
BspEI TCCGGA 1 cut(s) 893
BspFNI CGCG 2 cut(s) 533, 665
BspLI GGNNCC 2 cut(s) 178, 804
BspMI ACCTGC 2 cut(s) 107, 590
BspPI GGATC 4 cut(s) 257, 797, 810, 992
BspT107I GGYRCC 1 cut(s) 176
BspTNI GGTCTC 1 cut(s) 598
BsrI ACTGG 2 cut(s) 761, 826
BssECI CCNNGG 3 cut(s) 794, 871, 1001
BssMI GATC 5 cut(s) 3, 249, 625, 802, 997
BssNI GRCGYC 1 cut(s) 177
BssT1I CCWWGG 2 cut(s) 794, 871
Bst2UI CCWGG 2 cut(s) 810, 1002
Bst4CI ACNGT 6 cut(s) 101, 479, 619, 769, 948, 1177
Bst6I CTCTTC 2 cut(s) 419, 551
BstACI GRCGYC 1 cut(s) 177
BstDEI CTNAG 5 cut(s) 137, 161, 595, 919, 1161
BstDSI CCRYGG 1 cut(s) 794
BstFNI CGCG 2 cut(s) 533, 665
BstH2I RGCGCY 4 cut(s) 180, 187, 651, 852
BstHHI GCGC 5 cut(s) 179, 186, 650, 742, 851
BstKTI GATC 5 cut(s) 6, 252, 628, 805, 1000
BstMAI GTCTC 1 cut(s) 598
BstMBI GATC 5 cut(s) 3, 249, 625, 802, 997
BstNI CCWGG 2 cut(s) 810, 1002
BstSCI CCNGG 3 cut(s) 207, 808, 1000
BstUI CGCG 2 cut(s) 533, 665
BstV2I GAAGAC 1 cut(s) 758
BstX2I RGATCY 3 cut(s) 625, 802, 997
BstYI RGATCY 3 cut(s) 625, 802, 997
BsuI GTATCC 1 cut(s) 1159
BsuRI GGCC 1 cut(s) 207
BtgI CCRYGG 1 cut(s) 794
BtgZI GCGATG 1 cut(s) 233
BtsIMutI CAGTG 4 cut(s) 106, 484, 765, 833
BveI ACCTGC 2 cut(s) 107, 590
CaiI CAGNNNCTG 1 cut(s) 767
CfoI GCGC 5 cut(s) 179, 186, 650, 742, 851
Cfr13I GGNCC 3 cut(s) 206, 729, 891
CviAII CATG 5 cut(s) 235, 333, 433, 795, 932
DdeI CTNAG 5 cut(s) 137, 161, 595, 919, 1161
DinI GGCGCC 1 cut(s) 178
DpnI GATC 5 cut(s) 5, 251, 627, 804, 999
DpnII GATC 5 cut(s) 3, 249, 625, 802, 997
Eam1104I CTCTTC 2 cut(s) 419, 551
EarI CTCTTC 2 cut(s) 419, 551
Eco130I CCWWGG 2 cut(s) 794, 871
Eco31I GGTCTC 1 cut(s) 598
Eco32I GATATC 1 cut(s) 835
Eco47I GGWCC 2 cut(s) 729, 891
Eco57I CTGAAG 2 cut(s) 153, 532
EcoRII CCWGG 2 cut(s) 808, 1000
EcoRV GATATC 1 cut(s) 835
EcoT14I CCWWGG 2 cut(s) 794, 871
EgeI GGCGCC 1 cut(s) 178
EheI GGCGCC 1 cut(s) 178
ErhI CCWWGG 2 cut(s) 794, 871
FaeI CATG 5 cut(s) 238, 336, 436, 798, 935
FalI AAGNNNNNCTT 2 cut(s) 1088, 1120
FaqI GGGAC 1 cut(s) 659
FatI CATG 5 cut(s) 234, 332, 432, 794, 931
FspBI CTAG 6 cut(s) 344, 390, 584, 629, 927, 971
GlaI GCGC 5 cut(s) 178, 185, 649, 741, 850
HaeII RGCGCY 4 cut(s) 180, 187, 651, 852
HaeIII GGCC 1 cut(s) 207
HapII CCGG 4 cut(s) 209, 374, 386, 894
HhaI GCGC 5 cut(s) 179, 186, 650, 742, 851
Hin1I GRCGYC 1 cut(s) 177
Hin1II CATG 5 cut(s) 238, 336, 436, 798, 935
Hin6I GCGC 5 cut(s) 177, 184, 648, 740, 849
HinP1I GCGC 5 cut(s) 177, 184, 648, 740, 849
HinfI GANTC 3 cut(s) 53, 316, 1151
HpaII CCGG 4 cut(s) 209, 374, 386, 894
Hpy166II GTNNAC 3 cut(s) 688, 1054, 1093
Hpy188I TCNGA 7 cut(s) 33, 140, 172, 551, 789, 997, 1156
Hpy188III TCNNGA 3 cut(s) 629, 894, 1009
Hpy8I GTNNAC 3 cut(s) 688, 1054, 1093
HpyAV CCTTC 2 cut(s) 167, 235
HpyCH4III ACNGT 6 cut(s) 101, 479, 619, 769, 948, 1177
HpyCH4V TGCA 3 cut(s) 107, 659, 712
HpyF3I CTNAG 5 cut(s) 137, 161, 595, 919, 1161
Hsp92I GRCGYC 1 cut(s) 177
Hsp92II CATG 5 cut(s) 238, 336, 436, 798, 935
HspAI GCGC 5 cut(s) 177, 184, 648, 740, 849
KasI GGCGCC 1 cut(s) 176
Kpn2I TCCGGA 1 cut(s) 893
Kzo9I GATC 5 cut(s) 3, 249, 625, 802, 997
LmnI GCTCC 1 cut(s) 896
LweI GCATC 2 cut(s) 94, 780
MaeI CTAG 6 cut(s) 344, 390, 584, 629, 927, 971
MaeIII GTNAC 6 cut(s) 325, 511, 566, 619, 763, 1027
MalI GATC 5 cut(s) 5, 251, 627, 804, 999
MboI GATC 5 cut(s) 3, 249, 625, 802, 997
MboII GAAGA 6 cut(s) 143, 362, 406, 538, 763, 766
MfeI CAATTG 1 cut(s) 724
MflI RGATCY 3 cut(s) 625, 802, 997
MluCI AATT 5 cut(s) 229, 366, 504, 637, 724
Mly113I GGCGCC 1 cut(s) 177
MlyI GAGTC 1 cut(s) 1160
MmeI TCCRAC 2 cut(s) 265, 780
MnlI CCTC 6 cut(s) 29, 134, 393, 893, 928, 1046
MroI TCCGGA 1 cut(s) 893
MseI TTAA 3 cut(s) 291, 354, 1115
MslI CAYNNNNRTG 2 cut(s) 13, 477
MspI CCGG 4 cut(s) 209, 374, 386, 894
MspR9I CCNGG 3 cut(s) 209, 810, 1002
MunI CAATTG 1 cut(s) 724
MvaI CCWGG 2 cut(s) 810, 1002
MvnI CGCG 2 cut(s) 533, 665
NarI GGCGCC 1 cut(s) 177
NciI CCSGG 1 cut(s) 209
NcoI CCATGG 1 cut(s) 794
NdeII GATC 5 cut(s) 3, 249, 625, 802, 997
NlaIII CATG 5 cut(s) 238, 336, 436, 798, 935
NlaIV GGNNCC 2 cut(s) 178, 804
NmuCI GTSAC 3 cut(s) 325, 511, 763
OliI CACNNNNGTG 1 cut(s) 477
PaqCI CACCTGC 1 cut(s) 590
PfeI GAWTC 2 cut(s) 53, 316
PleI GAGTC 1 cut(s) 1159
PluTI GGCGCC 1 cut(s) 180
PpsI GAGTC 1 cut(s) 1159
Psp6I CCWGG 2 cut(s) 808, 1000
PspGI CCWGG 2 cut(s) 808, 1000
PspN4I GGNNCC 2 cut(s) 178, 804
PspPI GGNCC 3 cut(s) 206, 729, 891
PstNI CAGNNNCTG 1 cut(s) 767
PsuI RGATCY 3 cut(s) 625, 802, 997
RseI CAYNNNNRTG 2 cut(s) 13, 477
SaqAI TTAA 3 cut(s) 291, 354, 1115
Sau3AI GATC 5 cut(s) 3, 249, 625, 802, 997
Sau96I GGNCC 3 cut(s) 206, 729, 891
SchI GAGTC 1 cut(s) 1160
ScrFI CCNGG 3 cut(s) 209, 810, 1002
SfaNI GCATC 2 cut(s) 94, 780
SfoI GGCGCC 1 cut(s) 178
SinI GGWCC 2 cut(s) 729, 891
SmiMI CAYNNNNRTG 2 cut(s) 13, 477
SmlI CTYRAG 1 cut(s) 844
SmoI CTYRAG 1 cut(s) 844
Sse9I AATT 5 cut(s) 229, 366, 504, 637, 724
SsiI CCGC 2 cut(s) 533, 732
SspDI GGCGCC 1 cut(s) 176
SspMI CTAG 6 cut(s) 344, 390, 584, 629, 927, 971
StyD4I CCNGG 3 cut(s) 207, 808, 1000
StyI CCWWGG 2 cut(s) 794, 871
TaaI ACNGT 6 cut(s) 101, 479, 619, 769, 948, 1177
TasI AATT 5 cut(s) 229, 366, 504, 637, 724
TfiI GAWTC 2 cut(s) 53, 316
Tru1I TTAA 3 cut(s) 291, 354, 1115
Tru9I TTAA 3 cut(s) 291, 354, 1115
TscAI CASTG 4 cut(s) 106, 484, 772, 833
TseFI GTSAC 3 cut(s) 325, 511, 763
Tsp45I GTSAC 3 cut(s) 325, 511, 763
TspDTI ATGAA 5 cut(s) 45, 84, 223, 308, 529
TspRI CASTG 4 cut(s) 106, 484, 772, 833
VpaK11BI GGWCC 2 cut(s) 729, 891
XbaI TCTAGA 1 cut(s) 628
XcmI CCANNNNNNNNNTGG 2 cut(s) 32, 938
XspI CTAG 6 cut(s) 344, 390, 584, 629, 927, 971
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.