Rmu_ssc0000123.1_g000002

membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000123.1
OG0010952
Physical Location & Seq
Forward (+)
4023 .. 6267
2245 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000123.1_g000002.1.cds

Sequence Viewer

Length: 1179 bp
atggctttgcttgattcattgttggggaatgaagccaagagatttgtgaaaaggaaagatagtgatgcaggagaagcaggtaaagcacttgaagaactcagaggttctctctacaatgagcttagaacttcagaaggcgccaagcgccaacagcaacgattttgtggcccggtggtggcgatgaccttcaatttcatggtgtctgtggggatcattctggcaaataaacttgtacatgccttatcctttccttcttgcctttctgtaatggggagagttggatttaagttcccgatctttctcacattgattcattatgtgacagcatggttgcttctagccatttttaagacactcacaattcttccggtttctcctccggctagaaccactcctttcacttctctcttctctttaggtgctgtcatggcttttgcttctggtcttgccaatacaagcctaaaacacaacagtgtcggtttctaccagatggctaaaattgctgtcactcctaccattgttctcgcggagttcattctcttcagaaaaaccatttcttgtaacaaggttttagctctagctgtggtctcagcaggtgtggcagtagcaacagtaacagatctagagttcaatttgtttggcgctatgattgcaatcgcgtggatagtcccaagtgctgtaaacaaaatcctatggtctaatctgcaacagcaaggcaattggaccgcccttgcgctgatgtggaagactaccccagtcactatattcttcttattagctctgatgccatggttggatccaccaggagttctactattccagtgggatatcaataactcaagcgccattcttgtatcagcaatccttggttttctcctccaatggtccggagcattggcacttggggcaacctcagcaactagccatgttgttcttggacagttcaaaacttgtgtgatattactagggggctatttccttttcaaatcagatccagggttcgtgagcctttgtggggctgttacagccctctgtggaatgtctgtttacacatcactcaacctgaaaaagccacaagacaatgtgaccaagcaaaccttaccgacattaaaacccaaaaccattgaccaagaaaatgtagtagagtcagaagctaaggataccccaactgttgtttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPT

Protein Analysis

392

Amino Acids

42.4

Weight (kDa)

9.34

Isoelectric Point (pI)

35.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010952)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55950 AT5G55950 AT5G55950 AT5G55950
fragaria_vesca FvH4_3g42500 FvH4_3g42500
malus_domestica MD11G1035900.v1.1
prunus_persica Prupe.6G026800_v2.0.a1 Prupe.6G026800_v2.0.a1
pyrus_communis pycom11g02970
rosa_chinensis RchiOBHm_Chr5g0076161
rosa_laevigata RLG00000036606
rosa_multiflora Rmu_ssc0000123.1_g000002
rosa_roxburghii Rroxscaffold_1G00005650
rosa_rugosa Rorug05G0445200
rosa_samantha Rh5AG501300 Rh5BG522800 Rh5CG546600 Rh5DG535200
rosa_wichuraiana Rw5G046530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 584
Acc36I ACCTGC 2 cut(s) 68, 584
AccB1I GGYRCC 1 cut(s) 137
AccII CGCG 2 cut(s) 527, 659
AccIII TCCGGA 1 cut(s) 887
AciI CCGC 2 cut(s) 527, 726
AclWI GGATC 4 cut(s) 218, 791, 804, 986
AcuI CTGAAG 2 cut(s) 114, 526
AcyI GRCGYC 1 cut(s) 138
AfaI GTAC 1 cut(s) 234
AfiI CCNNNNNNNGG 2 cut(s) 175, 1015
AgsI TTSAA 5 cut(s) 92, 190, 631, 946, 985
AjnI CCWGG 2 cut(s) 802, 994
AleI CACNNNNGTG 1 cut(s) 471
AluBI AGCT 5 cut(s) 121, 575, 581, 779, 1154
AluI AGCT 5 cut(s) 121, 575, 581, 779, 1154
Alw26I GTCTC 1 cut(s) 592
AlwI GGATC 4 cut(s) 218, 791, 804, 986
Aor13HI TCCGGA 1 cut(s) 887
AoxI GGCC 1 cut(s) 166
AspLEI GCGC 5 cut(s) 140, 147, 644, 736, 845
AspS9I GGNCC 3 cut(s) 167, 723, 885
AsuC2I CCSGG 1 cut(s) 170
AvaII GGWCC 2 cut(s) 723, 885
BamHI GGATCC 1 cut(s) 796
BanI GGYRCC 1 cut(s) 137
BbsI GAAGAC 1 cut(s) 752
BbvCI CCTCAGC 1 cut(s) 913
BccI CCATC 1 cut(s) 484
BciT130I CCWGG 2 cut(s) 804, 996
BciVI GTATCC 1 cut(s) 1153
BcnI CCSGG 1 cut(s) 170
BcoDI GTCTC 1 cut(s) 592
BfaI CTAG 6 cut(s) 338, 384, 578, 623, 921, 965
BfoI RGCGCY 4 cut(s) 141, 148, 645, 846
BfuAI ACCTGC 2 cut(s) 68, 584
BfuI GTATCC 1 cut(s) 1153
BglII AGATCT 1 cut(s) 619
Bme1390I CCNGG 3 cut(s) 170, 804, 996
Bme18I GGWCC 2 cut(s) 723, 885
BmgT120I GGNCC 3 cut(s) 167, 723, 885
BmiI GGNNCC 2 cut(s) 139, 798
BmrFI CCNGG 3 cut(s) 170, 804, 996
BmrI ACTGGG 1 cut(s) 749
BmsI GCATC 2 cut(s) 55, 774
BmuI ACTGGG 1 cut(s) 749
BpiI GAAGAC 1 cut(s) 752
BplI GAGNNNNNCTC 2 cut(s) 93, 125
Bpu10I CCTNAGC 2 cut(s) 913, 1155
BpuEI CTTGAG 1 cut(s) 823
BpuMI CCSGG 1 cut(s) 170
BsaBI GATNNNNATC 1 cut(s) 653
BsaHI GRCGYC 1 cut(s) 138
BsaI GGTCTC 1 cut(s) 592
BsaJI CCNNGG 3 cut(s) 788, 865, 995
BsaWI WCCGGW 2 cut(s) 367, 887
Bsc4I CCNNNNNNNGG 2 cut(s) 175, 1015
Bse1I ACTGG 2 cut(s) 755, 820
Bse8I GATNNNNATC 1 cut(s) 653
BseAI TCCGGA 1 cut(s) 887
BseBI CCWGG 2 cut(s) 804, 996
BseDI CCNNGG 3 cut(s) 788, 865, 995
BseJI GATNNNNATC 1 cut(s) 653
BseLI CCNNNNNNNGG 2 cut(s) 175, 1015
BseMII CTCAG 3 cut(s) 112, 603, 927
BseNI ACTGG 2 cut(s) 755, 820
BseRI GAGGAG 2 cut(s) 366, 866
Bsh1236I CGCG 2 cut(s) 527, 659
BshFI GGCC 1 cut(s) 168
BshNI GGYRCC 1 cut(s) 137
BsiSI CCGG 4 cut(s) 170, 368, 380, 888
BslFI GGGAC 1 cut(s) 653
BslI CCNNNNNNNGG 2 cut(s) 175, 1015
BsmAI GTCTC 1 cut(s) 592
BsmFI GGGAC 1 cut(s) 653
BsnI GGCC 1 cut(s) 168
Bso31I GGTCTC 1 cut(s) 592
Bsp13I TCCGGA 1 cut(s) 887
Bsp1407I TGTACA 1 cut(s) 232
Bsp143I GATC 5 cut(s) 210, 294, 619, 796, 991
Bsp19I CCATGG 1 cut(s) 788
BspACI CCGC 2 cut(s) 527, 726
BspANI GGCC 1 cut(s) 168
BspCNI CTCAG 3 cut(s) 111, 602, 926
BspEI TCCGGA 1 cut(s) 887
BspFNI CGCG 2 cut(s) 527, 659
BspLI GGNNCC 2 cut(s) 139, 798
BspMI ACCTGC 2 cut(s) 68, 584
BspPI GGATC 4 cut(s) 218, 791, 804, 986
BspT107I GGYRCC 1 cut(s) 137
BspTNI GGTCTC 1 cut(s) 592
BsrGI TGTACA 1 cut(s) 232
BsrI ACTGG 2 cut(s) 755, 820
BssECI CCNNGG 3 cut(s) 788, 865, 995
BssMI GATC 5 cut(s) 210, 294, 619, 796, 991
BssNI GRCGYC 1 cut(s) 138
BssT1I CCWWGG 2 cut(s) 788, 865
Bst2UI CCWGG 2 cut(s) 804, 996
Bst4CI ACNGT 4 cut(s) 473, 613, 942, 1171
Bst6I CTCTTC 2 cut(s) 413, 545
BstACI GRCGYC 1 cut(s) 138
BstAUI TGTACA 1 cut(s) 232
BstDEI CTNAG 5 cut(s) 98, 122, 589, 913, 1155
BstDSI CCRYGG 1 cut(s) 788
BstFNI CGCG 2 cut(s) 527, 659
BstH2I RGCGCY 4 cut(s) 141, 148, 645, 846
BstHHI GCGC 5 cut(s) 140, 147, 644, 736, 845
BstKTI GATC 5 cut(s) 213, 297, 622, 799, 994
BstMAI GTCTC 1 cut(s) 592
BstMBI GATC 5 cut(s) 210, 294, 619, 796, 991
BstNI CCWGG 2 cut(s) 804, 996
BstNSI RCATGY 1 cut(s) 239
BstSCI CCNGG 3 cut(s) 168, 802, 994
BstUI CGCG 2 cut(s) 527, 659
BstV2I GAAGAC 1 cut(s) 752
BstX2I RGATCY 3 cut(s) 619, 796, 991
BstYI RGATCY 3 cut(s) 619, 796, 991
BsuI GTATCC 1 cut(s) 1153
BsuRI GGCC 1 cut(s) 168
BtgI CCRYGG 1 cut(s) 788
BtgZI GCGATG 1 cut(s) 194
BtsIMutI CAGTG 2 cut(s) 478, 827
BveI ACCTGC 2 cut(s) 68, 584
CfoI GCGC 5 cut(s) 140, 147, 644, 736, 845
Cfr13I GGNCC 3 cut(s) 167, 723, 885
Csp6I GTAC 1 cut(s) 233
CviAII CATG 6 cut(s) 196, 236, 327, 427, 789, 926
CviQI GTAC 1 cut(s) 233
DdeI CTNAG 5 cut(s) 98, 122, 589, 913, 1155
DinI GGCGCC 1 cut(s) 139
DpnI GATC 5 cut(s) 212, 296, 621, 798, 993
DpnII GATC 5 cut(s) 210, 294, 619, 796, 991
Eam1104I CTCTTC 2 cut(s) 413, 545
EarI CTCTTC 2 cut(s) 413, 545
Eco130I CCWWGG 2 cut(s) 788, 865
Eco31I GGTCTC 1 cut(s) 592
Eco32I GATATC 1 cut(s) 829
Eco47I GGWCC 2 cut(s) 723, 885
Eco57I CTGAAG 2 cut(s) 114, 526
EcoRII CCWGG 2 cut(s) 802, 994
EcoRV GATATC 1 cut(s) 829
EcoT14I CCWWGG 2 cut(s) 788, 865
EgeI GGCGCC 1 cut(s) 139
EheI GGCGCC 1 cut(s) 139
ErhI CCWWGG 2 cut(s) 788, 865
FaeI CATG 6 cut(s) 199, 239, 330, 430, 792, 929
FalI AAGNNNNNCTT 2 cut(s) 1082, 1114
FaqI GGGAC 1 cut(s) 653
FatI CATG 6 cut(s) 195, 235, 326, 426, 788, 925
FspBI CTAG 6 cut(s) 338, 384, 578, 623, 921, 965
GlaI GCGC 5 cut(s) 139, 146, 643, 735, 844
HaeII RGCGCY 4 cut(s) 141, 148, 645, 846
HaeIII GGCC 1 cut(s) 168
HapII CCGG 4 cut(s) 170, 368, 380, 888
HhaI GCGC 5 cut(s) 140, 147, 644, 736, 845
Hin1I GRCGYC 1 cut(s) 138
Hin1II CATG 6 cut(s) 199, 239, 330, 430, 792, 929
Hin6I GCGC 5 cut(s) 138, 145, 642, 734, 843
HinP1I GCGC 5 cut(s) 138, 145, 642, 734, 843
HinfI GANTC 3 cut(s) 14, 310, 1145
HpaII CCGG 4 cut(s) 170, 368, 380, 888
Hpy166II GTNNAC 2 cut(s) 682, 1048
Hpy188I TCNGA 6 cut(s) 101, 133, 545, 783, 991, 1150
Hpy188III TCNNGA 4 cut(s) 292, 623, 888, 1003
Hpy8I GTNNAC 2 cut(s) 682, 1048
HpyAV CCTTC 3 cut(s) 128, 196, 261
HpyCH4III ACNGT 4 cut(s) 473, 613, 942, 1171
HpyCH4V TGCA 3 cut(s) 68, 653, 706
HpyF3I CTNAG 5 cut(s) 98, 122, 589, 913, 1155
Hsp92I GRCGYC 1 cut(s) 138
Hsp92II CATG 6 cut(s) 199, 239, 330, 430, 792, 929
HspAI GCGC 5 cut(s) 138, 145, 642, 734, 843
KasI GGCGCC 1 cut(s) 137
Kpn2I TCCGGA 1 cut(s) 887
Kzo9I GATC 5 cut(s) 210, 294, 619, 796, 991
LmnI GCTCC 1 cut(s) 890
LweI GCATC 2 cut(s) 55, 774
MaeI CTAG 6 cut(s) 338, 384, 578, 623, 921, 965
MaeIII GTNAC 7 cut(s) 319, 505, 560, 613, 757, 1021, 1084
MalI GATC 5 cut(s) 212, 296, 621, 798, 993
MboI GATC 5 cut(s) 210, 294, 619, 796, 991
MboII GAAGA 6 cut(s) 104, 356, 400, 532, 757, 760
MfeI CAATTG 1 cut(s) 718
MflI RGATCY 3 cut(s) 619, 796, 991
MluCI AATT 5 cut(s) 190, 360, 498, 631, 718
Mly113I GGCGCC 1 cut(s) 138
MlyI GAGTC 1 cut(s) 1154
MmeI TCCRAC 2 cut(s) 259, 774
MnlI CCTC 5 cut(s) 95, 387, 887, 922, 1040
MroI TCCGGA 1 cut(s) 887
MseI TTAA 3 cut(s) 285, 348, 1109
MslI CAYNNNNRTG 1 cut(s) 471
MspI CCGG 4 cut(s) 170, 368, 380, 888
MspR9I CCNGG 3 cut(s) 170, 804, 996
MunI CAATTG 1 cut(s) 718
MvaI CCWGG 2 cut(s) 804, 996
MvnI CGCG 2 cut(s) 527, 659
NarI GGCGCC 1 cut(s) 138
NciI CCSGG 1 cut(s) 170
NcoI CCATGG 1 cut(s) 788
NdeII GATC 5 cut(s) 210, 294, 619, 796, 991
NlaIII CATG 6 cut(s) 199, 239, 330, 430, 792, 929
NlaIV GGNNCC 2 cut(s) 139, 798
NmuCI GTSAC 4 cut(s) 319, 505, 757, 1084
NspI RCATGY 1 cut(s) 239
OliI CACNNNNGTG 1 cut(s) 471
PaqCI CACCTGC 1 cut(s) 584
PfeI GAWTC 2 cut(s) 14, 310
PleI GAGTC 1 cut(s) 1153
PluTI GGCGCC 1 cut(s) 141
PpsI GAGTC 1 cut(s) 1153
Psp6I CCWGG 2 cut(s) 802, 994
PspGI CCWGG 2 cut(s) 802, 994
PspN4I GGNNCC 2 cut(s) 139, 798
PspPI GGNCC 3 cut(s) 167, 723, 885
PsuI RGATCY 3 cut(s) 619, 796, 991
RsaI GTAC 1 cut(s) 234
RsaNI GTAC 1 cut(s) 233
RseI CAYNNNNRTG 1 cut(s) 471
SaqAI TTAA 3 cut(s) 285, 348, 1109
Sau3AI GATC 5 cut(s) 210, 294, 619, 796, 991
Sau96I GGNCC 3 cut(s) 167, 723, 885
SchI GAGTC 1 cut(s) 1154
ScrFI CCNGG 3 cut(s) 170, 804, 996
SfaNI GCATC 2 cut(s) 55, 774
SfoI GGCGCC 1 cut(s) 139
SinI GGWCC 2 cut(s) 723, 885
SmiMI CAYNNNNRTG 1 cut(s) 471
SmlI CTYRAG 1 cut(s) 838
SmoI CTYRAG 1 cut(s) 838
Sse9I AATT 5 cut(s) 190, 360, 498, 631, 718
SsiI CCGC 2 cut(s) 527, 726
SspDI GGCGCC 1 cut(s) 137
SspMI CTAG 6 cut(s) 338, 384, 578, 623, 921, 965
StyD4I CCNGG 3 cut(s) 168, 802, 994
StyI CCWWGG 2 cut(s) 788, 865
TaaI ACNGT 4 cut(s) 473, 613, 942, 1171
TasI AATT 5 cut(s) 190, 360, 498, 631, 718
TatI WGTACW 1 cut(s) 232
TfiI GAWTC 2 cut(s) 14, 310
Tru1I TTAA 3 cut(s) 285, 348, 1109
Tru9I TTAA 3 cut(s) 285, 348, 1109
TscAI CASTG 2 cut(s) 478, 827
TseFI GTSAC 4 cut(s) 319, 505, 757, 1084
Tsp45I GTSAC 4 cut(s) 319, 505, 757, 1084
TspDTI ATGAA 5 cut(s) 6, 45, 184, 302, 523
TspRI CASTG 2 cut(s) 478, 827
VpaK11BI GGWCC 2 cut(s) 723, 885
XbaI TCTAGA 1 cut(s) 622
XceI RCATGY 1 cut(s) 239
XcmI CCANNNNNNNNNTGG 1 cut(s) 932
XspI CTAG 6 cut(s) 338, 384, 578, 623, 921, 965
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.