RchiOBHm_Chr5g0076161

membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0010952
Physical Location & Seq
Forward (+)
81857085 .. 81859634
2550 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35085

Sequence Viewer

Length: 1293 bp
ATGATCCCATATATATGCCTCTGCCATTGTCAGATTGGAATGGCTTTGCTTGATTCATTGTTGGGGAATGAAGCCAAGAGATTTGTGAAAAGGAAAGACAGTGATGCAGGAGAAGCAGGTAAAGCACTTGAAGAACTCAGAGGTTCTCTCTACAATGAGCTTAGAACTTCAGAAGGCGCCAAGCGCCAACAGCAACGATTTTGTGGCCCGGTGGTGGCGATGACCTTCAATTTCATGGTGTCTGTGGGGATCATTCTGGCAAATAAACTTGTAATGGGGAGAGTTGGATTTAAGTTTCCAATCTTTCTCACATTGATTCATTATGTGACAGCATGGTTGCTTCTAGCCATTTTTAAGACACTCTCAATTCTTCCGGTTTCTCCTCCGGCTAGAACTACTCCTTTTACTTCTCTCTTCTCTTTAGGTGCTGTCATGGCTTTTGCTTCTGGTCTTGCCAATACAAGCCTAAAACACAACAGGTTCTACTTAGGTCTCTTGTCTTTTATATATGATGTACAAAATTGTTACAAACTCAATATGTTGTTGCCTACTTTCCTCACCATTCTGCTCATTAATTGCAGTGTTGGTTTCTACCAGATGGCTAAAATTGCTGTCACTCCTACCATTGTTCTCGCGGAGTTCATTCTCTTCAGAAAAACCATTTCTTTCTTGCTTGTGCAGGTTTTAGCTCTAGCTGTGGTCTCAGCAGGTGTGGCAGTAGCAACAGTAACAGATCTAGAGTTCAATTTGTTTGGCGCTATGATTGCAATCGCGTGGATAGTCCCAAGTGCTGTAAACAAAATCCTATGGTCTAATCTGCAACAGCAAGGCAATTGGACCGCCCTTGCGCTGATGTGGAAGACTACCCCAGTCACTATATTCTTCTTATTAGCTCTGATGCCATGGTTGGATCCACCAGGAGTTCTACTATTCCAGTGGGATATCAATAACTCAAGCGCCATTCTTGTATCAGCAATCCTTGGTTTTCTCCTCCAATGGTCCGGAGCATTGGCACTTGGGGCAACCTCAGCAACTAGCCATGTTGTTCTTGGACAGTTCAAAACTTGTGTGATATTACTAGGGGGCTATTTCCTTTTCAAATCAGATCCAGGGTTCGTGAGCCTTTGTGGGGCTGTTGCAGCCCTCTGTGGAATGTCTGTTTACACATCACTAAACCTGAAAAAGCCACAAGACAATGCGACCAAGCAAACCTTACCGACATTAAAACCCAAAACCATTGACCAAGAAAATGTAGTAGAGTCAGAAGCCAAGGATAACCCAACTGTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPT

Protein Analysis

430

Amino Acids

46.97

Weight (kDa)

9.14

Isoelectric Point (pI)

33.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010952)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55950 AT5G55950 AT5G55950 AT5G55950
fragaria_vesca FvH4_3g42500 FvH4_3g42500
malus_domestica MD11G1035900.v1.1
prunus_persica Prupe.6G026800_v2.0.a1 Prupe.6G026800_v2.0.a1
pyrus_communis pycom11g02970
rosa_chinensis RchiOBHm_Chr5g0076161
rosa_laevigata RLG00000036606
rosa_multiflora Rmu_ssc0000123.1_g000002
rosa_roxburghii Rroxscaffold_1G00005650
rosa_rugosa Rorug05G0445200
rosa_samantha Rh5AG501300 Rh5BG522800 Rh5CG546600 Rh5DG535200
rosa_wichuraiana Rw5G046530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 698
Acc36I ACCTGC 3 cut(s) 107, 670, 698
AccB1I GGYRCC 1 cut(s) 176
AccII CGCG 2 cut(s) 635, 773
AccIII TCCGGA 1 cut(s) 1001
AciI CCGC 2 cut(s) 635, 840
AclWI GGATC 4 cut(s) 257, 905, 918, 1100
AcuI CTGAAG 2 cut(s) 153, 634
AcyI GRCGYC 1 cut(s) 177
AfaI GTAC 1 cut(s) 516
AfiI CCNNNNNNNGG 2 cut(s) 214, 1129
AgsI TTSAA 5 cut(s) 131, 229, 745, 1060, 1099
AjnI CCWGG 2 cut(s) 916, 1108
AluBI AGCT 4 cut(s) 160, 689, 695, 893
AluI AGCT 4 cut(s) 160, 689, 695, 893
Alw26I GTCTC 2 cut(s) 497, 706
AlwI GGATC 4 cut(s) 257, 905, 918, 1100
Aor13HI TCCGGA 1 cut(s) 1001
AoxI GGCC 1 cut(s) 205
ApeKI GCWGC 1 cut(s) 1139
AseI ATTAAT 1 cut(s) 573
AspLEI GCGC 5 cut(s) 179, 186, 758, 850, 959
AspS9I GGNCC 3 cut(s) 206, 837, 999
AsuC2I CCSGG 1 cut(s) 209
AsuHPI GGTGA 1 cut(s) 550
AvaII GGWCC 2 cut(s) 837, 999
BamHI GGATCC 1 cut(s) 910
BanI GGYRCC 1 cut(s) 176
BbsI GAAGAC 1 cut(s) 866
BbvCI CCTCAGC 1 cut(s) 1027
BbvI GCAGC 1 cut(s) 1151
BccI CCATC 1 cut(s) 592
BciT130I CCWGG 2 cut(s) 918, 1110
BcnI CCSGG 1 cut(s) 209
BcoDI GTCTC 2 cut(s) 497, 706
BfaI CTAG 6 cut(s) 344, 390, 692, 737, 1035, 1079
BfoI RGCGCY 4 cut(s) 180, 187, 759, 960
BfuAI ACCTGC 3 cut(s) 107, 670, 698
BglII AGATCT 1 cut(s) 733
BisI GCNGC 1 cut(s) 1140
BlsI GCNGC 1 cut(s) 1141
Bme1390I CCNGG 3 cut(s) 209, 918, 1110
Bme18I GGWCC 2 cut(s) 837, 999
BmgT120I GGNCC 3 cut(s) 206, 837, 999
BmiI GGNNCC 2 cut(s) 178, 912
BmrFI CCNGG 3 cut(s) 209, 918, 1110
BmrI ACTGGG 1 cut(s) 863
BmsI GCATC 2 cut(s) 94, 888
BmuI ACTGGG 1 cut(s) 863
BpiI GAAGAC 1 cut(s) 866
BplI GAGNNNNNCTC 2 cut(s) 132, 164
Bpu10I CCTNAGC 1 cut(s) 1027
BpuEI CTTGAG 1 cut(s) 937
BpuMI CCSGG 1 cut(s) 209
BsaBI GATNNNNATC 1 cut(s) 767
BsaHI GRCGYC 1 cut(s) 177
BsaI GGTCTC 2 cut(s) 497, 706
BsaJI CCNNGG 4 cut(s) 902, 979, 1109, 1269
BsaWI WCCGGW 2 cut(s) 373, 1001
Bsc4I CCNNNNNNNGG 2 cut(s) 214, 1129
Bse1I ACTGG 2 cut(s) 869, 934
Bse8I GATNNNNATC 1 cut(s) 767
BseAI TCCGGA 1 cut(s) 1001
BseBI CCWGG 2 cut(s) 918, 1110
BseDI CCNNGG 4 cut(s) 902, 979, 1109, 1269
BseJI GATNNNNATC 1 cut(s) 767
BseLI CCNNNNNNNGG 2 cut(s) 214, 1129
BseMII CTCAG 3 cut(s) 151, 717, 1041
BseNI ACTGG 2 cut(s) 869, 934
BseRI GAGGAG 2 cut(s) 372, 980
BseXI GCAGC 1 cut(s) 1151
BsgI GTGCAG 1 cut(s) 698
Bsh1236I CGCG 2 cut(s) 635, 773
BshFI GGCC 1 cut(s) 207
BshNI GGYRCC 1 cut(s) 176
BsiSI CCGG 4 cut(s) 209, 374, 386, 1002
BslFI GGGAC 1 cut(s) 767
BslI CCNNNNNNNGG 2 cut(s) 214, 1129
BsmAI GTCTC 2 cut(s) 497, 706
BsmFI GGGAC 1 cut(s) 767
BsnI GGCC 1 cut(s) 207
Bso31I GGTCTC 2 cut(s) 497, 706
Bsp13I TCCGGA 1 cut(s) 1001
Bsp1407I TGTACA 1 cut(s) 514
Bsp143I GATC 5 cut(s) 3, 249, 733, 910, 1105
Bsp19I CCATGG 1 cut(s) 902
BspACI CCGC 2 cut(s) 635, 840
BspANI GGCC 1 cut(s) 207
BspCNI CTCAG 3 cut(s) 150, 716, 1040
BspEI TCCGGA 1 cut(s) 1001
BspFNI CGCG 2 cut(s) 635, 773
BspLI GGNNCC 2 cut(s) 178, 912
BspMI ACCTGC 3 cut(s) 107, 670, 698
BspPI GGATC 4 cut(s) 257, 905, 918, 1100
BspT107I GGYRCC 1 cut(s) 176
BspTNI GGTCTC 2 cut(s) 497, 706
BsrGI TGTACA 1 cut(s) 514
BsrI ACTGG 2 cut(s) 869, 934
BssECI CCNNGG 4 cut(s) 902, 979, 1109, 1269
BssMI GATC 5 cut(s) 3, 249, 733, 910, 1105
BssNI GRCGYC 1 cut(s) 177
BssT1I CCWWGG 3 cut(s) 902, 979, 1269
Bst2UI CCWGG 2 cut(s) 918, 1110
Bst4CI ACNGT 4 cut(s) 101, 727, 1056, 1285
Bst6I CTCTTC 2 cut(s) 419, 653
BstACI GRCGYC 1 cut(s) 177
BstAUI TGTACA 1 cut(s) 514
BstDEI CTNAG 5 cut(s) 137, 161, 487, 703, 1027
BstDSI CCRYGG 1 cut(s) 902
BstFNI CGCG 2 cut(s) 635, 773
BstH2I RGCGCY 4 cut(s) 180, 187, 759, 960
BstHHI GCGC 5 cut(s) 179, 186, 758, 850, 959
BstKTI GATC 5 cut(s) 6, 252, 736, 913, 1108
BstMAI GTCTC 2 cut(s) 497, 706
BstMBI GATC 5 cut(s) 3, 249, 733, 910, 1105
BstNI CCWGG 2 cut(s) 918, 1110
BstSCI CCNGG 3 cut(s) 207, 916, 1108
BstUI CGCG 2 cut(s) 635, 773
BstV1I GCAGC 1 cut(s) 1151
BstV2I GAAGAC 1 cut(s) 866
BstX2I RGATCY 3 cut(s) 733, 910, 1105
BstYI RGATCY 3 cut(s) 733, 910, 1105
BsuRI GGCC 1 cut(s) 207
BtgI CCRYGG 1 cut(s) 902
BtgZI GCGATG 1 cut(s) 233
BtsI GCAGTG 1 cut(s) 586
BtsIMutI CAGTG 3 cut(s) 106, 586, 941
BveI ACCTGC 3 cut(s) 107, 670, 698
CfoI GCGC 5 cut(s) 179, 186, 758, 850, 959
Cfr13I GGNCC 3 cut(s) 206, 837, 999
Csp6I GTAC 1 cut(s) 515
CviAII CATG 5 cut(s) 235, 333, 433, 903, 1040
CviQI GTAC 1 cut(s) 515
DdeI CTNAG 5 cut(s) 137, 161, 487, 703, 1027
DinI GGCGCC 1 cut(s) 178
DpnI GATC 5 cut(s) 5, 251, 735, 912, 1107
DpnII GATC 5 cut(s) 3, 249, 733, 910, 1105
Eam1104I CTCTTC 2 cut(s) 419, 653
EarI CTCTTC 2 cut(s) 419, 653
Eco130I CCWWGG 3 cut(s) 902, 979, 1269
Eco31I GGTCTC 2 cut(s) 497, 706
Eco32I GATATC 1 cut(s) 943
Eco47I GGWCC 2 cut(s) 837, 999
Eco57I CTGAAG 2 cut(s) 153, 634
EcoRII CCWGG 2 cut(s) 916, 1108
EcoRV GATATC 1 cut(s) 943
EcoT14I CCWWGG 3 cut(s) 902, 979, 1269
EgeI GGCGCC 1 cut(s) 178
EheI GGCGCC 1 cut(s) 178
ErhI CCWWGG 3 cut(s) 902, 979, 1269
FaeI CATG 5 cut(s) 238, 336, 436, 906, 1043
FalI AAGNNNNNCTT 2 cut(s) 1196, 1228
FaqI GGGAC 1 cut(s) 767
FatI CATG 5 cut(s) 234, 332, 432, 902, 1039
Fnu4HI GCNGC 1 cut(s) 1140
Fsp4HI GCNGC 1 cut(s) 1140
FspBI CTAG 6 cut(s) 344, 390, 692, 737, 1035, 1079
GlaI GCGC 5 cut(s) 178, 185, 757, 849, 958
GluI GCNGC 1 cut(s) 1140
HaeII RGCGCY 4 cut(s) 180, 187, 759, 960
HaeIII GGCC 1 cut(s) 207
HapII CCGG 4 cut(s) 209, 374, 386, 1002
HhaI GCGC 5 cut(s) 179, 186, 758, 850, 959
Hin1I GRCGYC 1 cut(s) 177
Hin1II CATG 5 cut(s) 238, 336, 436, 906, 1043
Hin6I GCGC 5 cut(s) 177, 184, 756, 848, 957
HinP1I GCGC 5 cut(s) 177, 184, 756, 848, 957
HinfI GANTC 3 cut(s) 53, 316, 1259
HpaII CCGG 4 cut(s) 209, 374, 386, 1002
HphI GGTGA 1 cut(s) 550
Hpy166II GTNNAC 2 cut(s) 796, 1162
Hpy188I TCNGA 7 cut(s) 33, 140, 172, 653, 897, 1105, 1264
Hpy188III TCNNGA 3 cut(s) 737, 1002, 1117
Hpy8I GTNNAC 2 cut(s) 796, 1162
HpyAV CCTTC 2 cut(s) 167, 235
HpyCH4III ACNGT 4 cut(s) 101, 727, 1056, 1285
HpyCH4V TGCA 6 cut(s) 107, 579, 679, 767, 820, 1139
HpyF3I CTNAG 5 cut(s) 137, 161, 487, 703, 1027
Hsp92I GRCGYC 1 cut(s) 177
Hsp92II CATG 5 cut(s) 238, 336, 436, 906, 1043
HspAI GCGC 5 cut(s) 177, 184, 756, 848, 957
KasI GGCGCC 1 cut(s) 176
Kpn2I TCCGGA 1 cut(s) 1001
Kzo9I GATC 5 cut(s) 3, 249, 733, 910, 1105
LmnI GCTCC 1 cut(s) 1004
Lsp1109I GCAGC 1 cut(s) 1151
LweI GCATC 2 cut(s) 94, 888
MaeI CTAG 6 cut(s) 344, 390, 692, 737, 1035, 1079
MaeIII GTNAC 5 cut(s) 325, 524, 613, 727, 871
MalI GATC 5 cut(s) 5, 251, 735, 912, 1107
MboI GATC 5 cut(s) 3, 249, 733, 910, 1105
MboII GAAGA 6 cut(s) 143, 362, 406, 640, 871, 874
MfeI CAATTG 1 cut(s) 832
MflI RGATCY 3 cut(s) 733, 910, 1105
MluCI AATT 7 cut(s) 229, 366, 520, 574, 606, 745, 832
Mly113I GGCGCC 1 cut(s) 177
MlyI GAGTC 1 cut(s) 1268
MmeI TCCRAC 2 cut(s) 265, 888
MnlI CCTC 7 cut(s) 29, 134, 393, 566, 1001, 1036, 1154
MroI TCCGGA 1 cut(s) 1001
MseI TTAA 4 cut(s) 291, 354, 573, 1223
MslI CAYNNNNRTG 1 cut(s) 13
MspI CCGG 4 cut(s) 209, 374, 386, 1002
MspR9I CCNGG 3 cut(s) 209, 918, 1110
MunI CAATTG 1 cut(s) 832
MvaI CCWGG 2 cut(s) 918, 1110
MvnI CGCG 2 cut(s) 635, 773
NarI GGCGCC 1 cut(s) 177
NciI CCSGG 1 cut(s) 209
NcoI CCATGG 1 cut(s) 902
NdeII GATC 5 cut(s) 3, 249, 733, 910, 1105
NlaIII CATG 5 cut(s) 238, 336, 436, 906, 1043
NlaIV GGNNCC 2 cut(s) 178, 912
NmuCI GTSAC 3 cut(s) 325, 613, 871
PaqCI CACCTGC 1 cut(s) 698
PfeI GAWTC 2 cut(s) 53, 316
PkrI GCNGC 1 cut(s) 1141
PleI GAGTC 1 cut(s) 1267
PluTI GGCGCC 1 cut(s) 180
PpsI GAGTC 1 cut(s) 1267
PshBI ATTAAT 1 cut(s) 573
Psp6I CCWGG 2 cut(s) 916, 1108
PspGI CCWGG 2 cut(s) 916, 1108
PspN4I GGNNCC 2 cut(s) 178, 912
PspPI GGNCC 3 cut(s) 206, 837, 999
PsuI RGATCY 3 cut(s) 733, 910, 1105
RsaI GTAC 1 cut(s) 516
RsaNI GTAC 1 cut(s) 515
RseI CAYNNNNRTG 1 cut(s) 13
SaqAI TTAA 4 cut(s) 291, 354, 573, 1223
SatI GCNGC 1 cut(s) 1140
Sau3AI GATC 5 cut(s) 3, 249, 733, 910, 1105
Sau96I GGNCC 3 cut(s) 206, 837, 999
SchI GAGTC 1 cut(s) 1268
ScrFI CCNGG 3 cut(s) 209, 918, 1110
SfaNI GCATC 2 cut(s) 94, 888
SfoI GGCGCC 1 cut(s) 178
SinI GGWCC 2 cut(s) 837, 999
SmiMI CAYNNNNRTG 1 cut(s) 13
SmlI CTYRAG 1 cut(s) 952
SmoI CTYRAG 1 cut(s) 952
Sse9I AATT 7 cut(s) 229, 366, 520, 574, 606, 745, 832
SsiI CCGC 2 cut(s) 635, 840
SspDI GGCGCC 1 cut(s) 176
SspMI CTAG 6 cut(s) 344, 390, 692, 737, 1035, 1079
StyD4I CCNGG 3 cut(s) 207, 916, 1108
StyI CCWWGG 3 cut(s) 902, 979, 1269
TaaI ACNGT 4 cut(s) 101, 727, 1056, 1285
TasI AATT 7 cut(s) 229, 366, 520, 574, 606, 745, 832
TatI WGTACW 1 cut(s) 514
TfiI GAWTC 2 cut(s) 53, 316
Tru1I TTAA 4 cut(s) 291, 354, 573, 1223
Tru9I TTAA 4 cut(s) 291, 354, 573, 1223
TscAI CASTG 3 cut(s) 106, 586, 941
TseFI GTSAC 3 cut(s) 325, 613, 871
TseI GCWGC 1 cut(s) 1139
Tsp45I GTSAC 3 cut(s) 325, 613, 871
TspDTI ATGAA 5 cut(s) 45, 84, 223, 308, 631
TspRI CASTG 3 cut(s) 106, 586, 941
VpaK11BI GGWCC 2 cut(s) 837, 999
VspI ATTAAT 1 cut(s) 573
XbaI TCTAGA 1 cut(s) 736
XcmI CCANNNNNNNNNTGG 2 cut(s) 32, 1046
XspI CTAG 6 cut(s) 344, 390, 692, 737, 1035, 1079
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.