FvH4_3g44100

Amino acid permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0018825
Physical Location & Seq
Forward (+)
36784780 .. 36788028
3249 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g44100.t1

Sequence Viewer

Length: 1392 bp
ATGACGACGAGCATTTTGAGTACCGGCCGGATGTTAGATGATGATGGCAGACCACAAAGAACTGGGACAGTGTGGAGTGCATGTGCACACATAATCACAACAGCAATTGGGGTCGGAGTTCTGAGTCTTGCTTGGGCAATGGCACAACTTGGCTGGGTTGCTGGCAGTCTTCTTTTCTTGTTTATCTCCGCAACTTCGCTCTTCACAGCTACTCTGTTAGAAGATTGCTACAGGAGTCCAGACCCTGTAACTGGAAGGAGGAACTACACTTACATGGAGGTTGTCAAATCCTATTTAGGTGGAACAATGTACAAGTTCTGTGGATTCATCCTATATACGGATCTCGTTGCTGTTTCAGTCGGCTATTCAATCACTGCGGCAAAAAGCATGTTGGCTATCCAGAAATCCATCTGCCGTCAGAGAAGTGGCGGTGCTTCCTCGTGCAGGTTTTCCAACATACCGCACGGACTTGGATTTGGGATTGTGCAGGTTGTTCTATCTCAAATCCCAAACTTTCACAAGCTGGCTTGGCTCTCAAAGCTTGCAGCACTCATGTCTTTTGGCTATGCTTCTATAGCAATTGGCCTCTCTATTGAAAAAATAATCTCAGGGCCGGAAGGGAAAACATCTCTCTCTGGATTGCACCAGGCTCCATCGGAAAAGGTGTGGAGTATATTCACAGCAGCTGGAGACATCGCGCTTTCTTACGTATTTACAGTGACGGTCATTGAGATCCAAGACACTCTAAAGTCATCACCACCAGAAAACAAAGTGATGAAGAAGGCCACCAAAATAGGGGTTATGGTGATGACTATATTGTTCATCTTGTGTGGTACCTTGGGTTATGCTGCATTTGGAGACGAAAAAGTTCCAGAAAATCTCATGGCTGGTTTTCTATCGCAGAAGGCCTTCTGGATAGTGGACTTGGCCAATGTCTTCGTTGTTGTGCACTTGGTGGGTGCATTTCAGGTTTTTTCCCAACCAGTGTACCGCTTGATTGAAGTGTGGGCTGCTAGGAGATGGCCTAAGTCTGGCTTTGTAACCAAGGAAACGGCTGTCAGCTTTGGAAGGGTGAAGTTTGACGTAAACATGCTTCGCTTGTCCTGCAGAACAGGCTTTGTGCTTTTGACTACTGTGATTGCCACAGCACTGCCTTTTTTCTCTGATATGTTGGCACTACTTGGCGCTATCGGCTTTTGGCCGCTTACCATCTACTTCCCGTTGCAGCTGCACATTGCACAGAAGAAGATTCAACGAGCATCCCTCCGTTGGTTTGGCCTGCAGCTGCTGAGTGTTGTGTGCTTCCTAATTTCAGTTGCTGCAGCCTGTGGTGCAGTCCACGGTGTGCACAAGGGTCTGAAGGTCAACCGACCCTTTCAGTTCAAGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

464

Amino Acids

50.53

Weight (kDa)

9.57

Isoelectric Point (pI)

38.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 20 - 447 7e-100 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018825)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g44100
rosa_chinensis RchiOBHm_Chr6g0298541
rosa_laevigata RLG00000011505
rosa_multiflora Rmu_sc0004500.1_g000006
rosa_samantha Rh6BG400200 Rh6CG406100 Rh6DG392600 Rh6DG392700
rosa_wichuraiana Rw6G034300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 435, 478
Acc65I GGTACC 1 cut(s) 833
AccB1I GGYRCC 1 cut(s) 833
AccII CGCG 1 cut(s) 698
AciI CCGC 6 cut(s) 189, 377, 429, 461, 991, 1202
AclWI GGATC 2 cut(s) 348, 727
AcoI YGGCCR 3 cut(s) 25, 927, 1199
AcuI CTGAAG 1 cut(s) 1379
AdeI CACNNNGTG 2 cut(s) 955, 1345
AfaI GTAC 4 cut(s) 22, 311, 835, 989
AfiI CCNNNNNNNGG 6 cut(s) 251, 337, 444, 795, 1031, 1269
AgsI TTSAA 5 cut(s) 369, 596, 1001, 1253, 1384
AjnI CCWGG 1 cut(s) 645
AluBI AGCT 7 cut(s) 209, 523, 541, 686, 1062, 1228, 1285
AluI AGCT 7 cut(s) 209, 523, 541, 686, 1062, 1228, 1285
Alw21I GWGCWC 3 cut(s) 88, 951, 1350
Alw26I GTCTC 2 cut(s) 684, 852
Alw44I GTGCAC 3 cut(s) 84, 947, 1346
AlwI GGATC 2 cut(s) 348, 727
AlwNI CAGNNNCTG 4 cut(s) 245, 686, 1288, 1319
AoxI GGCC 9 cut(s) 25, 583, 611, 783, 906, 927, 1022, 1199, 1276
ApaLI GTGCAC 3 cut(s) 84, 947, 1346
ArsI GACNNNNNNTTYG 1 cut(s) 30
Asp700I GAANNNNTTC 2 cut(s) 867, 908
Asp718I GGTACC 1 cut(s) 833
AspLEI GCGC 2 cut(s) 700, 1187
AspS9I GGNCC 1 cut(s) 611
AsuHPI GGTGA 3 cut(s) 747, 817, 1084
BaeGI GKGCMC 3 cut(s) 88, 951, 1350
BaeI ACNNNNGTAYC 2 cut(s) 971, 1004
BalI TGGCCA 1 cut(s) 929
BanI GGYRCC 1 cut(s) 833
BarI GAAGNNNNNNTAC 2 cut(s) 1077, 1109
BauI CACGAG 1 cut(s) 439
BbsI GAAGAC 2 cut(s) 161, 928
Bbv12I GWGCWC 3 cut(s) 88, 951, 1350
BccI CCATC 5 cut(s) 38, 416, 661, 1014, 1217
BceAI ACGGC 2 cut(s) 399, 1068
BciT130I CCWGG 1 cut(s) 647
BcoDI GTCTC 2 cut(s) 684, 852
BfaI CTAG 1 cut(s) 1014
BfmI CTRYAG 5 cut(s) 229, 573, 1105, 1280, 1320
BfoI RGCGCY 1 cut(s) 1188
BfuAI ACCTGC 2 cut(s) 435, 478
Bme1390I CCNGG 1 cut(s) 647
BmgT120I GGNCC 1 cut(s) 611
BmiI GGNNCC 2 cut(s) 651, 835
BmrFI CCNGG 1 cut(s) 647
BmrI ACTGGG 1 cut(s) 72
BmsI GCATC 1 cut(s) 1268
BmuI ACTGGG 1 cut(s) 72
BpiI GAAGAC 2 cut(s) 161, 928
BplI GAGNNNNNCTC 2 cut(s) 1248, 1280
BpmI CTGGAG 1 cut(s) 708
BsaAI YACGTR 1 cut(s) 709
BsaJI CCNNGG 3 cut(s) 837, 1044, 1339
BsaXI ACNNNNNCTCC 2 cut(s) 67, 97
Bsc4I CCNNNNNNNGG 6 cut(s) 251, 337, 444, 795, 1031, 1269
Bse118I RCCGGY 1 cut(s) 23
Bse1I ACTGG 3 cut(s) 67, 256, 983
Bse3DI GCAATG 2 cut(s) 144, 1233
BseBI CCWGG 1 cut(s) 647
BseDI CCNNGG 3 cut(s) 837, 1044, 1339
BseGI GGATG 3 cut(s) 36, 327, 1259
BseLI CCNNNNNNNGG 6 cut(s) 251, 337, 444, 795, 1031, 1269
BseMI GCAATG 2 cut(s) 144, 1233
BseMII CTCAG 3 cut(s) 113, 621, 1280
BseNI ACTGG 3 cut(s) 67, 256, 983
BseSI GKGCMC 3 cut(s) 88, 951, 1350
BseX3I CGGCCG 1 cut(s) 25
BseYI CCCAGC 1 cut(s) 153
BsgI GTGCAG 4 cut(s) 463, 506, 1214, 1353
Bsh1236I CGCG 1 cut(s) 698
Bsh1285I CGRYCG 1 cut(s) 28
BshFI GGCC 9 cut(s) 27, 585, 613, 785, 908, 929, 1024, 1201, 1278
BshNI GGYRCC 1 cut(s) 833
BsiEI CGRYCG 1 cut(s) 28
BsiHKAI GWGCWC 3 cut(s) 88, 951, 1350
BsiSI CCGG 3 cut(s) 24, 28, 614
BslFI GGGAC 1 cut(s) 79
BslI CCNNNNNNNGG 6 cut(s) 251, 337, 444, 795, 1031, 1269
BsmAI GTCTC 2 cut(s) 684, 852
BsmBI CGTCTC 1 cut(s) 852
BsmFI GGGAC 1 cut(s) 79
BsnI GGCC 9 cut(s) 27, 585, 613, 785, 908, 929, 1024, 1201, 1278
Bsp1286I GDGCHC 3 cut(s) 88, 951, 1350
Bsp1407I TGTACA 1 cut(s) 309
Bsp143I GATC 2 cut(s) 340, 732
BspACI CCGC 6 cut(s) 189, 377, 429, 461, 991, 1202
BspANI GGCC 9 cut(s) 27, 585, 613, 785, 908, 929, 1024, 1201, 1278
BspCNI CTCAG 3 cut(s) 114, 620, 1281
BspFNI CGCG 1 cut(s) 698
BspLI GGNNCC 2 cut(s) 651, 835
BspMAI CTGCAG 3 cut(s) 1109, 1284, 1324
BspMI ACCTGC 2 cut(s) 435, 478
BspPI GGATC 2 cut(s) 348, 727
BspQI GCTCTTC 1 cut(s) 206
BspT107I GGYRCC 1 cut(s) 833
BsrDI GCAATG 2 cut(s) 144, 1233
BsrFI RCCGGY 1 cut(s) 23
BsrGI TGTACA 1 cut(s) 309
BsrI ACTGG 3 cut(s) 67, 256, 983
BssAI RCCGGY 1 cut(s) 23
BssECI CCNNGG 3 cut(s) 837, 1044, 1339
BssMI GATC 2 cut(s) 340, 732
BssSI CACGAG 1 cut(s) 439
BssT1I CCWWGG 2 cut(s) 837, 1044
Bst2BI CACGAG 1 cut(s) 439
Bst2UI CCWGG 1 cut(s) 647
Bst4CI ACNGT 5 cut(s) 70, 718, 724, 1135, 1343
Bst6I CTCTTC 1 cut(s) 206
BstAUI TGTACA 1 cut(s) 309
BstBAI YACGTR 1 cut(s) 709
BstC8I GCNNGC 4 cut(s) 163, 525, 543, 1280
BstDEI CTNAG 4 cut(s) 122, 607, 1026, 1289
BstDSI CCRYGG 1 cut(s) 1339
BstENI CCTNNNNNAGG 1 cut(s) 442
BstF5I GGATG 3 cut(s) 36, 327, 1259
BstFNI CGCG 1 cut(s) 698
BstH2I RGCGCY 1 cut(s) 1188
BstHHI GCGC 2 cut(s) 700, 1187
BstKTI GATC 2 cut(s) 343, 735
BstMAI GTCTC 2 cut(s) 684, 852
BstMBI GATC 2 cut(s) 340, 732
BstMCI CGRYCG 1 cut(s) 28
BstMWI GCNNNNNNNGC 7 cut(s) 529, 538, 575, 1104, 1113, 1191, 1331
BstNI CCWGG 1 cut(s) 647
BstNSI RCATGY 3 cut(s) 84, 391, 1093
BstSCI CCNGG 1 cut(s) 645
BstSFI CTRYAG 5 cut(s) 229, 573, 1105, 1280, 1320
BstSLI GKGCMC 3 cut(s) 88, 951, 1350
BstSNI TACGTA 1 cut(s) 709
BstUI CGCG 1 cut(s) 698
BstV2I GAAGAC 2 cut(s) 161, 928
BstX2I RGATCY 2 cut(s) 340, 732
BstYI RGATCY 2 cut(s) 340, 732
BstZI CGGCCG 1 cut(s) 25
BsuRI GGCC 9 cut(s) 27, 585, 613, 785, 908, 929, 1024, 1201, 1278
BtgI CCRYGG 1 cut(s) 1339
BtgZI GCGATG 1 cut(s) 679
BtsCI GGATG 3 cut(s) 36, 327, 1259
BtsI GCAGTG 2 cut(s) 372, 1148
BtsIMutI CAGTG 5 cut(s) 75, 372, 723, 990, 1148
BveI ACCTGC 2 cut(s) 435, 478
Cac8I GCNNGC 4 cut(s) 163, 525, 543, 1280
CaiI CAGNNNCTG 4 cut(s) 245, 686, 1288, 1319
CfoI GCGC 2 cut(s) 700, 1187
Cfr10I RCCGGY 1 cut(s) 23
Cfr13I GGNCC 1 cut(s) 611
Csp6I GTAC 4 cut(s) 21, 310, 834, 988
CspCI CAANNNNNGTGG 2 cut(s) 301, 336
CviAII CATG 6 cut(s) 81, 274, 388, 553, 883, 1090
CviQI GTAC 4 cut(s) 21, 310, 834, 988
DdeI CTNAG 4 cut(s) 122, 607, 1026, 1289
DpnI GATC 2 cut(s) 342, 734
DpnII GATC 2 cut(s) 340, 732
DraIII CACNNNGTG 2 cut(s) 955, 1345
EaeI YGGCCR 3 cut(s) 25, 927, 1199
EagI CGGCCG 1 cut(s) 25
Eam1104I CTCTTC 1 cut(s) 206
EarI CTCTTC 1 cut(s) 206
EclXI CGGCCG 1 cut(s) 25
Eco105I TACGTA 1 cut(s) 709
Eco130I CCWWGG 2 cut(s) 837, 1044
Eco147I AGGCCT 1 cut(s) 908
Eco52I CGGCCG 1 cut(s) 25
Eco57I CTGAAG 1 cut(s) 1379
EcoNI CCTNNNNNAGG 1 cut(s) 442
EcoRII CCWGG 1 cut(s) 645
EcoT14I CCWWGG 2 cut(s) 837, 1044
ErhI CCWWGG 2 cut(s) 837, 1044
Esp3I CGTCTC 1 cut(s) 852
FaeI CATG 6 cut(s) 84, 277, 391, 556, 886, 1093
FalI AAGNNNNNCTT 2 cut(s) 1019, 1051
FaqI GGGAC 1 cut(s) 79
FatI CATG 6 cut(s) 80, 273, 387, 552, 882, 1089
FokI GGATG 3 cut(s) 43, 314, 1246
FspBI CTAG 1 cut(s) 1014
GlaI GCGC 2 cut(s) 699, 1186
GsaI CCCAGC 1 cut(s) 157
GsuI CTGGAG 1 cut(s) 708
HaeII RGCGCY 1 cut(s) 1188
HaeIII GGCC 9 cut(s) 27, 585, 613, 785, 908, 929, 1024, 1201, 1278
HapII CCGG 3 cut(s) 24, 28, 614
HhaI GCGC 2 cut(s) 700, 1187
Hin1II CATG 6 cut(s) 84, 277, 391, 556, 886, 1093
Hin6I GCGC 2 cut(s) 698, 1185
HinP1I GCGC 2 cut(s) 698, 1185
HincII GTYRAC 1 cut(s) 1366
HindII GTYRAC 1 cut(s) 1366
HindIII AAGCTT 1 cut(s) 539
HinfI GANTC 4 cut(s) 124, 235, 324, 1249
HpaII CCGG 3 cut(s) 24, 28, 614
HphI GGTGA 3 cut(s) 747, 817, 1084
Hpy166II GTNNAC 8 cut(s) 86, 922, 949, 988, 1087, 1339, 1348, 1366
Hpy188I TCNGA 6 cut(s) 116, 123, 420, 658, 1165, 1359
Hpy188III TCNNGA 5 cut(s) 239, 400, 636, 872, 913
Hpy8I GTNNAC 8 cut(s) 86, 922, 949, 988, 1087, 1339, 1348, 1366
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 7 cut(s) 249, 611, 775, 898, 919, 1062, 1354
HpyCH4III ACNGT 5 cut(s) 70, 718, 724, 1135, 1343
HpyCH4IV ACGT 2 cut(s) 708, 1083
HpyF10VI GCNNNNNNNGC 7 cut(s) 529, 538, 575, 1104, 1113, 1191, 1331
HpyF3I CTNAG 4 cut(s) 122, 607, 1026, 1289
HpySE526I ACGT 2 cut(s) 708, 1083
Hsp92II CATG 6 cut(s) 84, 277, 391, 556, 886, 1093
HspAI GCGC 2 cut(s) 698, 1185
KpnI GGTACC 1 cut(s) 837
Kzo9I GATC 2 cut(s) 340, 732
LguI GCTCTTC 1 cut(s) 206
LmnI GCTCC 1 cut(s) 655
LweI GCATC 1 cut(s) 1268
MaeI CTAG 1 cut(s) 1014
MaeII ACGT 2 cut(s) 708, 1083
MaeIII GTNAC 3 cut(s) 247, 718, 1039
MalI GATC 2 cut(s) 342, 734
MboI GATC 2 cut(s) 340, 732
MboII GAAGA 7 cut(s) 161, 193, 233, 790, 928, 1255, 1258
MfeI CAATTG 2 cut(s) 105, 579
MflI RGATCY 2 cut(s) 340, 732
MhlI GDGCHC 3 cut(s) 88, 951, 1350
MlsI TGGCCA 1 cut(s) 929
MluCI AATT 3 cut(s) 105, 579, 1308
MluNI TGGCCA 1 cut(s) 929
MlyI GAGTC 2 cut(s) 133, 244
MmeI TCCRAC 2 cut(s) 94, 477
MnlI CCTC 5 cut(s) 252, 271, 448, 596, 1274
Mox20I TGGCCA 1 cut(s) 929
MroXI GAANNNNTTC 2 cut(s) 867, 908
MscI TGGCCA 1 cut(s) 929
MslI CAYNNNNRTG 1 cut(s) 272
Msp20I TGGCCA 1 cut(s) 929
MspA1I CMGCKG 3 cut(s) 686, 1228, 1285
MspI CCGG 3 cut(s) 24, 28, 614
MspR9I CCNGG 1 cut(s) 647
MunI CAATTG 2 cut(s) 105, 579
MvaI CCWGG 1 cut(s) 647
MvnI CGCG 1 cut(s) 698
MwoI GCNNNNNNNGC 7 cut(s) 529, 538, 575, 1104, 1113, 1191, 1331
NdeII GATC 2 cut(s) 340, 732
NlaIII CATG 6 cut(s) 84, 277, 391, 556, 886, 1093
NlaIV GGNNCC 2 cut(s) 651, 835
NmuCI GTSAC 1 cut(s) 718
NspI RCATGY 3 cut(s) 84, 391, 1093
PceI AGGCCT 1 cut(s) 908
PciSI GCTCTTC 1 cut(s) 206
PdmI GAANNNNTTC 2 cut(s) 867, 908
PfeI GAWTC 2 cut(s) 324, 1249
PleI GAGTC 2 cut(s) 132, 243
PpsI GAGTC 2 cut(s) 132, 243
Ppu21I YACGTR 1 cut(s) 709
Psp6I CCWGG 1 cut(s) 645
PspFI CCCAGC 1 cut(s) 153
PspGI CCWGG 1 cut(s) 645
PspN4I GGNNCC 2 cut(s) 651, 835
PspPI GGNCC 1 cut(s) 611
PsrI GAACNNNNNNTAC 2 cut(s) 254, 286
PstI CTGCAG 3 cut(s) 1109, 1284, 1324
PstNI CAGNNNCTG 4 cut(s) 245, 686, 1288, 1319
PsuI RGATCY 2 cut(s) 340, 732
PvuII CAGCTG 3 cut(s) 686, 1228, 1285
RsaI GTAC 4 cut(s) 22, 311, 835, 989
RsaNI GTAC 4 cut(s) 21, 310, 834, 988
RseI CAYNNNNRTG 1 cut(s) 272
SapI GCTCTTC 1 cut(s) 206
Sau3AI GATC 2 cut(s) 340, 732
Sau96I GGNCC 1 cut(s) 611
SchI GAGTC 2 cut(s) 133, 244
ScrFI CCNGG 1 cut(s) 647
SduI GDGCHC 3 cut(s) 88, 951, 1350
SfaNI GCATC 1 cut(s) 1268
SfcI CTRYAG 5 cut(s) 229, 573, 1105, 1280, 1320
SmiMI CAYNNNNRTG 1 cut(s) 272
SnaBI TACGTA 1 cut(s) 709
Sse9I AATT 3 cut(s) 105, 579, 1308
SseBI AGGCCT 1 cut(s) 908
SsiI CCGC 6 cut(s) 189, 377, 429, 461, 991, 1202
SspMI CTAG 1 cut(s) 1014
StuI AGGCCT 1 cut(s) 908
StyD4I CCNGG 1 cut(s) 645
StyI CCWWGG 2 cut(s) 837, 1044
TaaI ACNGT 5 cut(s) 70, 718, 724, 1135, 1343
TaiI ACGT 2 cut(s) 711, 1086
TasI AATT 3 cut(s) 105, 579, 1308
TatI WGTACW 1 cut(s) 309
TauI GCSGC 2 cut(s) 380, 1204
TfiI GAWTC 2 cut(s) 324, 1249
TscAI CASTG 5 cut(s) 75, 379, 723, 990, 1155
TseFI GTSAC 1 cut(s) 718
Tsp45I GTSAC 1 cut(s) 718
TspDTI ATGAA 3 cut(s) 316, 791, 811
TspGWI ACGGA 3 cut(s) 353, 480, 1256
TspRI CASTG 5 cut(s) 75, 379, 723, 990, 1155
VneI GTGCAC 3 cut(s) 84, 947, 1346
XagI CCTNNNNNAGG 1 cut(s) 442
XceI RCATGY 3 cut(s) 84, 391, 1093
XmnI GAANNNNTTC 2 cut(s) 867, 908
XspI CTAG 1 cut(s) 1014
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.