FvH4_3g45830

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0012494
Physical Location & Seq
Forward (+)
38011327 .. 38014893
3567 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g45830.t1

Sequence Viewer

Length: 2361 bp
ATGGCTACTGATTCTCAGTGTTTTTGGGGTCTTTTCCTCTCACTTTCACTATACTTTATTCACTCAGATTCAACACCTCATGTGTACATAGTGTATTTGGGGCTCAGTCATAACATACATGAACCTCACCTAACTTCAGAACACCATGTTCAGCTCCTATCCAAAGTTTTTGCCAGTGAAGAAGATGTTAAACGGTCAATGCTTTATAGTTACAAGCATAGTTTCTCAGGCTTTTCAGCAAAAATCAATTCCTCACAAGCAGTCACCTTGGCCAAGGTGGAAGGAGTGATATCAGTTTTTAGAAGTAAGACAGTGCAATTGCACACAACTAGGAGTTGGGACTTCTTGGGCCTTCCAATGATCAGCAGCACTAGTACTGCCAGTGATGCGGCCTCTCCATTGCAACTAGCATATGGCCAAGATGTCATTGTTGGGGTTTTTGATACAGGTATATGGCCTGAATCGGAAAGTTTCCGAGAAGAGCCACATATGAGGCCTATCCCCTCAACTTGGAAGGGGGAGTGTGTGAAAGGAGAGATGTTTGAGCCAGCAAAAGCATGCAACCGTAAGCTAATTGGTGCTCGCTACTACCTGGAAGGCTTTGAAGAAGAATATGGGCCACTAAACACGAGTGGCAATCCAGAATTTCGATCAGCTAGAGACTTTCTAGGTCACGGAACACACACAGCCTCAACAGCAGTGGGTTCAGTAGTGAAACAAAATGCAAGTTTCTATGGCTTTGCAGAGGGCACTGCCCGGGGTGGAGCGCCTAAAGCACGTCTAGCAGTGTACAAAGTTTGCTGGGGAAAGAATTATAGTAGCAGGTGTACTGGAGCAGACATTCTTGCGGCTTTTGATGATGCTTTGCATGATGGAGTTCATGTTATTTCAGCGTCTATTGGATCCAGGCCACCACTGATACCGTTGTTTGCATCAGAAGGTGCTATCGGTTCGTTTCATGCAATGCAGTTGGGTGTGAGTGTGGTGTTCTCAGCAGGTAATGAAGGGCCAGATCCATCACAGGTCACAAATGTTGCTCCTTGGAGCCTGTGTGTTGCTGCTTCATCAATTGATAGAATGTTCCCAACCAGAGTTCTCCTAGACAATAAGCTCTCTATCATGGGAGAGAGCTTAATCACCACACCTATCAAAGCAAAATTGGCAGATGCGAGCGTATACTTCTATAATGGGATTTGCACGTCAAGGAATTGGAAAAAATCAAAATATGCTGCAAATATAGTAATCATATGCTTCTCAACCATAGGACCGTATGAAATTGGTGAGGCTGAAGTAGCTGCCAAGCTAGCCAATGCATCAGGTTTGATCTTTGTAGAACCAATGTCCAGGGAGATTGGTCCTGACATCATCCCCTCGGTTCATGTCAACATTGAGCAAGGGACTCGGATTTTGAACTATCTTTCTGAATCTCCGACGAAGCCTGTTGTGCAGATAGAAGCAAGTAAAACTATTATCGGGAAGGCACCGGCCCCTAGAGTCGCATACTTCTCTTCAAGAGGCCCAAACTCACTTACACCTGATATCCTCAAGCCAGATATAAGTGCTCCAGGGATAAACATATTAGCAGCATGGCCTAATCGAACTTCTCCAACTTTGAGACGCGATGATAATCGTTTAGTTAACTGGAATTTTCAGTCAGGAACATCAATGTCCTGTCCTCATGTCTCCGGGATTGTTGCTCTCATCAAATCTTCACACCCGGATTGGTCTCCTGCAGCCATTAAATCTGCTATCATGACCACAGCATACACAAAGGACACATCCTTTGACACCATACTAGCTGATGGACCCATGAAAGCCTCCGACCCTTTTGACTTGGGTGCCGGTCACATAGATCCCATCAAGGCAATGGATCCTGGGCTTGTCTACGACATGAAAACACGTGACTACGTTCAATTTCTATGCAACATTGGCTACACTGAAGAACAGATAAACATGATTGTTCCTTGCCCTTCAGGAACTGACACGAGTTGTCCACGAGTGCCGAAATCGAATTCCAATGTAAACTACCCATCGATCACAGTTTCAAATCTCCAGTCGACTGTGACAATCAAGAGAACCATGCGTAATGTGGGAGAGAATAAGAACGCCGTTTATTTTGGCACCATTTCTGAGCCTGATGGAGTGGAGGTAGTGATATGGCCTAGAGTTTTGGTATTCTCCTGGTTCAAAGCGGAAAACACATATTATGTAACACTGAAACCACAAAAGAAGTCTCAAGGAAGATATGATTTTGGAGAAATTGTTTGGTCTGATGGCTTCCACAAGGTTAGGAGTCCTTTGGTTGTTTGTGTCAACACTGCTCAAGATTCCAATGCATCCATGAACTTGAGCTACATGTGA

Protein Analysis

787

Amino Acids

85.85

Weight (kDa)

6.85

Isoelectric Point (pI)

40.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 28 - 108 2.5e-15 Peptidase inhibitor I9
Peptidase_S8 PF00082 139 - 593 2.5e-44 Subtilase family
fn3_6 PF17766 673 - 769 1.6e-25 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012494)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26330 AT4G26330
fragaria_vesca FvH4_3g45830
malus_domestica MD03G1002600.v1.1
prunus_persica Prupe.6G002300_v2.0.a1 Prupe.6G002300_v2.0.a1
pyrus_communis pycom03g00250
rosa_chinensis RchiOBHm_Chr5g0083911 RchiOBHm_Chr5g0083921
rosa_laevigata RLG00000037064
rosa_multiflora Rmu_sc0001137.1_g000006
rosa_roxburghii Rroxscaffold_1G00000690
rosa_rugosa Rorug05G0487600 Rorug05G0487600 Rorug05G0487600
rosa_samantha Rh5AG540400 Rh5BG565400 Rh5CG587300
rosa_wichuraiana Rw5G050240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 813
Acc36I ACCTGC 2 cut(s) 813, 986
AccB1I GGYRCC 3 cut(s) 1480, 1838, 2120
AccI GTMKAC 3 cut(s) 1176, 1884, 2057
AccII CGCG 1 cut(s) 1620
AciI CCGC 3 cut(s) 389, 848, 2192
AclWI GGATC 6 cut(s) 897, 910, 1007, 1847, 1865, 1878
AcoI YGGCCR 2 cut(s) 270, 415
AcsI RAATTY 3 cut(s) 644, 1645, 2011
AcuI CTGAAG 4 cut(s) 120, 1308, 1956, 1959
AcvI CACGTG 1 cut(s) 1901
AfaI GTAC 4 cut(s) 86, 376, 791, 829
AfiI CCNNNNNNNGG 1 cut(s) 510
AflIII ACRYGT 2 cut(s) 1898, 2355
AgsI TTSAA 7 cut(s) 72, 605, 1411, 1512, 1913, 2046, 2188
AhlI ACTAGT 1 cut(s) 371
AjiI CACGTC 2 cut(s) 779, 1200
AjnI CCWGG 6 cut(s) 591, 905, 1343, 1564, 1873, 2180
AluBI AGCT 9 cut(s) 154, 571, 656, 1111, 1131, 1295, 1303, 1799, 2352
AluI AGCT 9 cut(s) 154, 571, 656, 1111, 1131, 1295, 1303, 1799, 2352
Alw21I GWGCWC 2 cut(s) 583, 1564
Alw26I GTCTC 5 cut(s) 654, 1609, 1687, 1731, 2238
AlwI GGATC 6 cut(s) 897, 910, 1007, 1847, 1865, 1878
AlwNI CAGNNNCTG 1 cut(s) 1979
Ama87I CYCGRG 1 cut(s) 756
ApeKI GCWGC 6 cut(s) 366, 1058, 1229, 1295, 1583, 1733
ApoI RAATTY 3 cut(s) 644, 1645, 2011
ArsI GACNNNNNNTTYG 6 cut(s) 1390, 1422, 1766, 1798, 2039, 2071
AspLEI GCGC 1 cut(s) 769
AspS9I GGNCC 8 cut(s) 349, 617, 1007, 1265, 1355, 1486, 1517, 1805
AsuC2I CCSGG 4 cut(s) 757, 758, 1687, 1718
AsuHPI GGTGA 4 cut(s) 119, 256, 1129, 1292
AsuNHI GCTAGC 1 cut(s) 1303
AvaI CYCGRG 1 cut(s) 756
AvaII GGWCC 3 cut(s) 1265, 1355, 1805
BaeGI GKGCMC 1 cut(s) 752
BaeI ACNNNNGTAYC 2 cut(s) 911, 944
BalI TGGCCA 2 cut(s) 272, 417
BamHI GGATCC 2 cut(s) 902, 1870
BanI GGYRCC 3 cut(s) 1480, 1838, 2120
BanII GRGCYC 1 cut(s) 105
BauI CACGAG 3 cut(s) 628, 1984, 1995
BbrPI CACGTG 1 cut(s) 1901
Bbv12I GWGCWC 2 cut(s) 583, 1564
BbvI GCAGC 6 cut(s) 378, 1045, 1216, 1282, 1595, 1745
BccI CCATC 7 cut(s) 866, 1024, 1796, 1865, 2038, 2132, 2267
BceAI ACGGC 1 cut(s) 2093
BcgI CGANNNNNNTGC 2 cut(s) 1382, 1416
BciT130I CCWGG 6 cut(s) 593, 907, 1345, 1566, 1875, 2182
BclI TGATCA 1 cut(s) 360
BcnI CCSGG 4 cut(s) 757, 758, 1687, 1718
BcoDI GTCTC 5 cut(s) 654, 1609, 1687, 1731, 2238
BcuI ACTAGT 1 cut(s) 371
BfmI CTRYAG 1 cut(s) 1731
BfoI RGCGCY 1 cut(s) 770
BfuAI ACCTGC 2 cut(s) 813, 986
BisI GCNGC 8 cut(s) 367, 390, 849, 1059, 1230, 1296, 1584, 1734
BlsI GCNGC 8 cut(s) 368, 391, 850, 1060, 1231, 1297, 1585, 1735
BmcAI AGTACT 1 cut(s) 376
Bme18I GGWCC 3 cut(s) 1265, 1355, 1805
BmeT110I CYCGRG 1 cut(s) 756
BmgBI CACGTC 2 cut(s) 779, 1200
BmgT120I GGNCC 8 cut(s) 349, 617, 1007, 1265, 1355, 1486, 1517, 1805
BmiI GGNNCC 8 cut(s) 904, 1046, 1482, 1488, 1807, 1840, 1872, 2122
BmsI GCATC 6 cut(s) 376, 850, 941, 1156, 1322, 2345
BmtI GCTAGC 1 cut(s) 1307
BpmI CTGGAG 3 cut(s) 852, 1548, 2036
BpuEI CTTGAG 3 cut(s) 1529, 2220, 2307
BpuMI CCSGG 4 cut(s) 757, 758, 1687, 1718
Bsa29I ATCGAT 1 cut(s) 2033
BsaAI YACGTR 1 cut(s) 1901
BsaBI GATNNNNATC 1 cut(s) 1626
BsaI GGTCTC 1 cut(s) 1731
BsaJI CCNNGG 9 cut(s) 267, 273, 756, 757, 1040, 1344, 1371, 1565, 1874
BsaXI ACNNNNNCTCC 8 cut(s) 867, 897, 1036, 1066, 2133, 2163, 2247, 2277
Bsc4I CCNNNNNNNGG 1 cut(s) 510
Bse118I RCCGGY 2 cut(s) 1483, 1841
Bse1I ACTGG 5 cut(s) 174, 381, 835, 1646, 2053
Bse3DI GCAATG 3 cut(s) 398, 969, 1872
Bse8I GATNNNNATC 1 cut(s) 1626
BseBI CCWGG 6 cut(s) 593, 907, 1345, 1566, 1875, 2182
BseCI ATCGAT 1 cut(s) 2033
BseDI CCNNGG 9 cut(s) 267, 273, 756, 757, 1040, 1344, 1371, 1565, 1874
BseGI GGATG 3 cut(s) 1365, 1778, 2336
BseJI GATNNNNATC 1 cut(s) 1626
BseLI CCNNNNNNNGG 1 cut(s) 510
BseMI GCAATG 3 cut(s) 398, 969, 1872
BseMII CTCAG 6 cut(s) 29, 78, 118, 240, 1005, 2121
BseNI ACTGG 5 cut(s) 174, 381, 835, 1646, 2053
BseSI GKGCMC 1 cut(s) 752
BseXI GCAGC 6 cut(s) 378, 1045, 1216, 1282, 1595, 1745
BseYI CCCAGC 1 cut(s) 801
BsgI GTGCAG 1 cut(s) 1466
Bsh1236I CGCG 1 cut(s) 1620
BshNI GGYRCC 3 cut(s) 1480, 1838, 2120
BshVI ATCGAT 1 cut(s) 2033
BsiHKAI GWGCWC 2 cut(s) 583, 1564
BsiHKCI CYCGRG 1 cut(s) 756
BsiSI CCGG 5 cut(s) 757, 1484, 1686, 1718, 1842
BslFI GGGAC 2 cut(s) 353, 1411
BslI CCNNNNNNNGG 1 cut(s) 510
BsmAI GTCTC 5 cut(s) 654, 1609, 1687, 1731, 2238
BsmBI CGTCTC 1 cut(s) 1609
BsmFI GGGAC 2 cut(s) 353, 1411
Bso31I GGTCTC 1 cut(s) 1731
BsoBI CYCGRG 1 cut(s) 756
Bsp1286I GDGCHC 4 cut(s) 105, 583, 752, 1564
Bsp1407I TGTACA 2 cut(s) 84, 789
Bsp143I GATC 8 cut(s) 360, 650, 902, 1012, 1323, 1852, 1870, 2034
BspACI CCGC 3 cut(s) 389, 848, 2192
BspCNI CTCAG 6 cut(s) 28, 77, 117, 239, 1004, 2122
BspDI ATCGAT 1 cut(s) 2033
BspFNI CGCG 1 cut(s) 1620
BspHI TCATGA 1 cut(s) 1752
BspLI GGNNCC 8 cut(s) 904, 1046, 1482, 1488, 1807, 1840, 1872, 2122
BspMAI CTGCAG 1 cut(s) 1735
BspMI ACCTGC 2 cut(s) 813, 986
BspOI GCTAGC 1 cut(s) 1307
BspPI GGATC 6 cut(s) 897, 910, 1007, 1847, 1865, 1878
BspQI GCTCTTC 1 cut(s) 474
BspT107I GGYRCC 3 cut(s) 1480, 1838, 2120
BspTNI GGTCTC 1 cut(s) 1731
BsrDI GCAATG 3 cut(s) 398, 969, 1872
BsrFI RCCGGY 2 cut(s) 1483, 1841
BsrGI TGTACA 2 cut(s) 84, 789
BsrI ACTGG 5 cut(s) 174, 381, 835, 1646, 2053
BssAI RCCGGY 2 cut(s) 1483, 1841
BssECI CCNNGG 9 cut(s) 267, 273, 756, 757, 1040, 1344, 1371, 1565, 1874
BssMI GATC 8 cut(s) 360, 650, 902, 1012, 1323, 1852, 1870, 2034
BssNAI GTATAC 1 cut(s) 1177
BssSI CACGAG 3 cut(s) 628, 1984, 1995
BssT1I CCWWGG 3 cut(s) 267, 273, 1040
Bst1107I GTATAC 1 cut(s) 1177
Bst2BI CACGAG 3 cut(s) 628, 1984, 1995
Bst2UI CCWGG 6 cut(s) 593, 907, 1345, 1566, 1875, 2182
Bst4CI ACNGT 7 cut(s) 195, 313, 566, 924, 1269, 2041, 2062
Bst6I CTCTTC 2 cut(s) 474, 1513
BstAUI TGTACA 2 cut(s) 84, 789
BstBAI YACGTR 1 cut(s) 1901
BstC8I GCNNGC 5 cut(s) 549, 559, 583, 1171, 1305
BstDEI CTNAG 6 cut(s) 15, 64, 104, 226, 991, 2130
BstF5I GGATG 3 cut(s) 1365, 1778, 2336
BstFNI CGCG 1 cut(s) 1620
BstH2I RGCGCY 1 cut(s) 770
BstHHI GCGC 1 cut(s) 769
BstKTI GATC 8 cut(s) 363, 653, 905, 1015, 1326, 1855, 1873, 2037
BstMAI GTCTC 5 cut(s) 654, 1609, 1687, 1731, 2238
BstMBI GATC 8 cut(s) 360, 650, 902, 1012, 1323, 1852, 1870, 2034
BstMWI GCNNNNNNNGC 9 cut(s) 386, 695, 773, 782, 1160, 1292, 1304, 1444, 1929
BstNI CCWGG 6 cut(s) 593, 907, 1345, 1566, 1875, 2182
BstNSI RCATGY 2 cut(s) 561, 2359
BstSFI CTRYAG 1 cut(s) 1731
BstSLI GKGCMC 1 cut(s) 752
BstUI CGCG 1 cut(s) 1620
BstV1I GCAGC 6 cut(s) 378, 1045, 1216, 1282, 1595, 1745
BstX2I RGATCY 4 cut(s) 902, 1012, 1852, 1870
BstYI RGATCY 4 cut(s) 902, 1012, 1852, 1870
BstZ17I GTATAC 1 cut(s) 1177
Bsu15I ATCGAT 1 cut(s) 2033
BsuTUI ATCGAT 1 cut(s) 2033
BtgZI GCGATG 1 cut(s) 1635
BtrI CACGTC 2 cut(s) 779, 1200
BtsCI GGATG 3 cut(s) 1365, 1778, 2336
BtsI GCAGTG 4 cut(s) 705, 750, 792, 2316
BveI ACCTGC 2 cut(s) 813, 986
Cac8I GCNNGC 5 cut(s) 549, 559, 583, 1171, 1305
CaiI CAGNNNCTG 1 cut(s) 1979
CciI TCATGA 1 cut(s) 1752
CfoI GCGC 1 cut(s) 769
Cfr10I RCCGGY 2 cut(s) 1483, 1841
Cfr13I GGNCC 8 cut(s) 349, 617, 1007, 1265, 1355, 1486, 1517, 1805
Cfr9I CCCGGG 1 cut(s) 756
ClaI ATCGAT 1 cut(s) 2033
CseI GACGC 2 cut(s) 882, 1626
Csp6I GTAC 4 cut(s) 85, 375, 790, 828
CspCI CAANNNNNGTGG 2 cut(s) 681, 716
CviQI GTAC 4 cut(s) 85, 375, 790, 828
DdeI CTNAG 6 cut(s) 15, 64, 104, 226, 991, 2130
DpnI GATC 8 cut(s) 362, 652, 904, 1014, 1325, 1854, 1872, 2036
DpnII GATC 8 cut(s) 360, 650, 902, 1012, 1323, 1852, 1870, 2034
EaeI YGGCCR 2 cut(s) 270, 415
Eam1104I CTCTTC 2 cut(s) 474, 1513
EarI CTCTTC 2 cut(s) 474, 1513
Eco130I CCWWGG 3 cut(s) 267, 273, 1040
Eco147I AGGCCT 1 cut(s) 496
Eco24I GRGCYC 1 cut(s) 105
Eco31I GGTCTC 1 cut(s) 1731
Eco32I GATATC 2 cut(s) 291, 1540
Eco47I GGWCC 3 cut(s) 1265, 1355, 1805
Eco57I CTGAAG 4 cut(s) 120, 1308, 1956, 1959
Eco72I CACGTG 1 cut(s) 1901
Eco88I CYCGRG 1 cut(s) 756
EcoRI GAATTC 1 cut(s) 2011
EcoRII CCWGG 6 cut(s) 591, 905, 1343, 1564, 1873, 2180
EcoRV GATATC 2 cut(s) 291, 1540
EcoT14I CCWWGG 3 cut(s) 267, 273, 1040
EcoT22I ATGCAT 2 cut(s) 1315, 2338
EcoT38I GRGCYC 1 cut(s) 105
ErhI CCWWGG 3 cut(s) 267, 273, 1040
Esp3I CGTCTC 1 cut(s) 1609
FaqI GGGAC 2 cut(s) 353, 1411
FauNDI CATATG 3 cut(s) 412, 489, 1247
FbaI TGATCA 1 cut(s) 360
FblI GTMKAC 3 cut(s) 1176, 1884, 2057
Fnu4HI GCNGC 8 cut(s) 367, 390, 849, 1059, 1230, 1296, 1584, 1734
FokI GGATG 3 cut(s) 1352, 1765, 2323
FriOI GRGCYC 1 cut(s) 105
Fsp4HI GCNGC 8 cut(s) 367, 390, 849, 1059, 1230, 1296, 1584, 1734
GlaI GCGC 1 cut(s) 768
GluI GCNGC 8 cut(s) 367, 390, 849, 1059, 1230, 1296, 1584, 1734
GsaI CCCAGC 1 cut(s) 805
GsuI CTGGAG 3 cut(s) 852, 1548, 2036
HaeII RGCGCY 1 cut(s) 770
HapII CCGG 5 cut(s) 757, 1484, 1686, 1718, 1842
HgaI GACGC 2 cut(s) 882, 1626
HhaI GCGC 1 cut(s) 769
Hin6I GCGC 1 cut(s) 767
HinP1I GCGC 1 cut(s) 767
HincII GTYRAC 4 cut(s) 1384, 1639, 2058, 2314
HindII GTYRAC 4 cut(s) 1384, 1639, 2058, 2314
HinfI GANTC 8 cut(s) 11, 68, 461, 1399, 1424, 1494, 2293, 2327
HpaI GTTAAC 1 cut(s) 1639
HpaII CCGG 5 cut(s) 757, 1484, 1686, 1718, 1842
HphI GGTGA 4 cut(s) 119, 256, 1129, 1292
Hpy188III TCNNGA 9 cut(s) 641, 1358, 1474, 1512, 1656, 1753, 1974, 2071, 2324
Hpy99I CGWCG 1 cut(s) 1435
HpyAV CCTTC 8 cut(s) 275, 362, 508, 590, 932, 998, 1471, 1980
HpyCH4III ACNGT 7 cut(s) 195, 313, 566, 924, 1269, 2041, 2062
HpyCH4IV ACGT 4 cut(s) 778, 1199, 1900, 1908
HpyF10VI GCNNNNNNNGC 9 cut(s) 386, 695, 773, 782, 1160, 1292, 1304, 1444, 1929
HpyF3I CTNAG 6 cut(s) 15, 64, 104, 226, 991, 2130
HpySE526I ACGT 4 cut(s) 778, 1199, 1900, 1908
HspAI GCGC 1 cut(s) 767
Ksp22I TGATCA 1 cut(s) 360
KspAI GTTAAC 1 cut(s) 1639
Kzo9I GATC 8 cut(s) 360, 650, 902, 1012, 1323, 1852, 1870, 2034
LguI GCTCTTC 1 cut(s) 474
LmnI GCTCC 6 cut(s) 159, 764, 833, 1042, 1044, 1567
Lsp1109I GCAGC 6 cut(s) 378, 1045, 1216, 1282, 1595, 1745
LweI GCATC 6 cut(s) 376, 850, 941, 1156, 1322, 2345
MaeII ACGT 4 cut(s) 778, 1199, 1900, 1908
MaeIII GTNAC 8 cut(s) 209, 262, 671, 1024, 1844, 1901, 2062, 2209
MalI GATC 8 cut(s) 362, 652, 904, 1014, 1325, 1854, 1872, 2036
MboI GATC 8 cut(s) 360, 650, 902, 1012, 1323, 1852, 1870, 2034
MboII GAAGA 9 cut(s) 191, 194, 491, 617, 620, 1500, 1701, 1952, 2253
MfeI CAATTG 2 cut(s) 317, 1068
MflI RGATCY 4 cut(s) 902, 1012, 1852, 1870
MhlI GDGCHC 4 cut(s) 105, 583, 752, 1564
MlsI TGGCCA 2 cut(s) 272, 417
MluNI TGGCCA 2 cut(s) 272, 417
MlyI GAGTC 3 cut(s) 1393, 1503, 2302
MmeI TCCRAC 3 cut(s) 1454, 1631, 1845
Mox20I TGGCCA 2 cut(s) 272, 417
Mph1103I ATGCAT 2 cut(s) 1315, 2338
MscI TGGCCA 2 cut(s) 272, 417
MseI TTAA 4 cut(s) 189, 1133, 1638, 1740
Msp20I TGGCCA 2 cut(s) 272, 417
MspI CCGG 5 cut(s) 757, 1484, 1686, 1718, 1842
MunI CAATTG 2 cut(s) 317, 1068
MvaI CCWGG 6 cut(s) 593, 907, 1345, 1566, 1875, 2182
MvnI CGCG 1 cut(s) 1620
MwoI GCNNNNNNNGC 9 cut(s) 386, 695, 773, 782, 1160, 1292, 1304, 1444, 1929
NciI CCSGG 4 cut(s) 757, 758, 1687, 1718
NdeI CATATG 3 cut(s) 412, 489, 1247
NdeII GATC 8 cut(s) 360, 650, 902, 1012, 1323, 1852, 1870, 2034
NheI GCTAGC 1 cut(s) 1303
NlaIV GGNNCC 8 cut(s) 904, 1046, 1482, 1488, 1807, 1840, 1872, 2122
NmuCI GTSAC 6 cut(s) 262, 671, 1024, 1844, 1901, 2062
NsiI ATGCAT 2 cut(s) 1315, 2338
NspI RCATGY 2 cut(s) 561, 2359
PaeI GCATGC 1 cut(s) 561
PagI TCATGA 1 cut(s) 1752
PaqCI CACCTGC 1 cut(s) 813
PceI AGGCCT 1 cut(s) 496
PciI ACATGT 1 cut(s) 2355
PciSI GCTCTTC 1 cut(s) 474
PfeI GAWTC 5 cut(s) 11, 68, 461, 1424, 2327
PfoI TCCNGGA 1 cut(s) 1685
PkrI GCNGC 8 cut(s) 368, 391, 850, 1060, 1231, 1297, 1585, 1735
PleI GAGTC 3 cut(s) 1393, 1502, 2301
PmaCI CACGTG 1 cut(s) 1901
PmlI CACGTG 1 cut(s) 1901
PpsI GAGTC 3 cut(s) 1393, 1502, 2301
Ppu21I YACGTR 1 cut(s) 1901
PscI ACATGT 1 cut(s) 2355
Psp6I CCWGG 6 cut(s) 591, 905, 1343, 1564, 1873, 2180
PspCI CACGTG 1 cut(s) 1901
PspFI CCCAGC 1 cut(s) 801
PspGI CCWGG 6 cut(s) 591, 905, 1343, 1564, 1873, 2180
PspN4I GGNNCC 8 cut(s) 904, 1046, 1482, 1488, 1807, 1840, 1872, 2122
PspPI GGNCC 8 cut(s) 349, 617, 1007, 1265, 1355, 1486, 1517, 1805
PsrI GAACNNNNNNTAC 1 cut(s) 2336
PstI CTGCAG 1 cut(s) 1735
PstNI CAGNNNCTG 1 cut(s) 1979
PsuI RGATCY 4 cut(s) 902, 1012, 1852, 1870
RsaI GTAC 4 cut(s) 86, 376, 791, 829
RsaNI GTAC 4 cut(s) 85, 375, 790, 828
SalI GTCGAC 1 cut(s) 2056
SapI GCTCTTC 1 cut(s) 474
SaqAI TTAA 4 cut(s) 189, 1133, 1638, 1740
SatI GCNGC 8 cut(s) 367, 390, 849, 1059, 1230, 1296, 1584, 1734
Sau3AI GATC 8 cut(s) 360, 650, 902, 1012, 1323, 1852, 1870, 2034
Sau96I GGNCC 8 cut(s) 349, 617, 1007, 1265, 1355, 1486, 1517, 1805
ScaI AGTACT 1 cut(s) 376
SchI GAGTC 3 cut(s) 1393, 1503, 2302
SduI GDGCHC 4 cut(s) 105, 583, 752, 1564
SfaNI GCATC 6 cut(s) 376, 850, 941, 1156, 1322, 2345
SfcI CTRYAG 1 cut(s) 1731
SinI GGWCC 3 cut(s) 1265, 1355, 1805
SmaI CCCGGG 1 cut(s) 758
SmlI CTYRAG 4 cut(s) 1544, 2235, 2322, 2347
SmoI CTYRAG 4 cut(s) 1544, 2235, 2322, 2347
SpeI ACTAGT 1 cut(s) 371
SphI GCATGC 1 cut(s) 561
SseBI AGGCCT 1 cut(s) 496
SsiI CCGC 3 cut(s) 389, 848, 2192
StuI AGGCCT 1 cut(s) 496
StyI CCWWGG 3 cut(s) 267, 273, 1040
TaaI ACNGT 7 cut(s) 195, 313, 566, 924, 1269, 2041, 2062
TaiI ACGT 4 cut(s) 781, 1202, 1903, 1911
TaqI TCGA 5 cut(s) 649, 1597, 2009, 2033, 2057
TatI WGTACW 4 cut(s) 84, 374, 789, 827
TauI GCSGC 2 cut(s) 392, 851
TfiI GAWTC 5 cut(s) 11, 68, 461, 1424, 2327
Tru1I TTAA 4 cut(s) 189, 1133, 1638, 1740
Tru9I TTAA 4 cut(s) 189, 1133, 1638, 1740
TseFI GTSAC 6 cut(s) 262, 671, 1024, 1844, 1901, 2062
TseI GCWGC 6 cut(s) 366, 1058, 1229, 1295, 1583, 1733
Tsp45I GTSAC 6 cut(s) 262, 671, 1024, 1844, 1901, 2062
TspGWI ACGGA 1 cut(s) 690
TspMI CCCGGG 1 cut(s) 756
VpaK11BI GGWCC 3 cut(s) 1265, 1355, 1805
XapI RAATTY 3 cut(s) 644, 1645, 2011
XceI RCATGY 2 cut(s) 561, 2359
XcmI CCANNNNNNNNNTGG 2 cut(s) 1864, 2086
XmaI CCCGGG 1 cut(s) 756
XmiI GTMKAC 3 cut(s) 1176, 1884, 2057
ZrmI AGTACT 1 cut(s) 376
Zsp2I ATGCAT 2 cut(s) 1315, 2338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.