Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0012494

Physical Location & Seq

Genomic Coordinates Reverse (-)

181963 .. 187600

Gene Structure 5638 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1002600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2352 bp

ATGGAGCAAAACTTTATTGAAAGGTATATGTGTGCTATCTACCAATATGTTTATATAGTCTATTTGGGGCACAACTACAACGTTCATGTTCCTCACCTCACGTCAAACCATCATCTTCAACTCCTTTCCAAAGTTTTTGCAAGTGAAGAAGATGTTAAGAAATCCTTGCTTTATAGCTACAAGCACAGTTTCTCGGGGTTTTCAGCTAAGCTCAATTCTTCTCAAGCAACCGCCTTGGCCCACATGGAAGAAGTGATATCAGTGTTTCGAAGTGAGACAATGCAGTTGCACACAACAAGAAGCTGGGACTTCTTAGGCCTTACCTTAAGCGGCAGCAGCACTAGTCCTGAAGGGACTCCATTGCAACTAGCCTATGGTGACGACATCATTGTTGGGGTCTTCGATACAGGTATATGGCCTGAATCCGATAGTTTCCAAGAAGAGCCACGGATGAGGCCTATCCCCTCAACTTGGAAGGGAGGGTGTGCTAGAGGAGAAATGTTTGAGCCAGAAAAAGCATGCAACCGCAAGCTAATTGGTGCTCGCTACTACCTCAAAGGTTTTGAAGAAGAATACGGGCCACTAAACAAGAGTGGGAATCCAGAATATCGATCAGCTAGAGATTTTTTAGGTCATGGTACACACACAGCCTCAACAGCAGTGGGTTCAATAGTGAAAAACAATGCAAGTTTCTTTGGCTTAGCACCAGGAACCGCTAGGGGAGGAGCGCCGAGGGCTCGTCTAGCGGTGTATAAAGTATGTTGGGGAAAGGACTATGATGGGAGGTGCTCTGAAGCAGACATTCTTGCAGCTTTCGATGATGCTTTGCACGATGGAGTTCACATGATATCAGCGTCATTTGGATCAAAGCCGCCATTGTTGCCGCTGTTTATATCTCAAGCTTCAATTGGTTCATTTCATGCAATGCAGTTAGGTGTTAGTGTGGTGTTCTCTGCAGGTAATGAAGGGCCAGATCCCTCACAGGTCTCAAATGTTGCTCCTTGGGGAATTTGTGTAGCTGCTTCCTCTGTTGATCGAATGTTTCCAACCCGAATAATCCTAGACAATAAGCTCTTTGTTATGGGGGAAAGCTTTATTACCACACCTATTAAGGCAAAATTAGCAGATGCAACCACATATTTTGTCAATGGGGTTTGCAGGCCAGAGAATTGGATAAAATCAAAGTTTGCGGTCGGAAGAGTAATTCTATGCTTCTCAAGCATAGGACCATACGAAATTGATGAGGCAGAAGTAGCTGCCAAGAAAGCGAATGCATCGGGTTTGATCTTTGTGGAAGCTGAGACGAGGCAGGTTGATATCGACATCATTCCCTCAGTCCATGTCAACCTTGAGCAGGGGACTAAAATCAAGCACTATCTTGCTCAATCTCCCATGAAACCTGTTGTGCAGATAGAGGCAAGTAAAACTGTCATCGGTAAGTATCCAGCGCCTAGAGTTGCATACTTCTCTTCGAGAGGACCAAGCTCACTTGCACCTGATATTCTCAAGCCAGACATAAGTGCTCCAGGGGTCAATATATTGGCAGCATGGCCTCCTCAAACATCTCCCACTTTGACACTTGACGATAAACGTTCTGTGAGCTGGAATTTTCAGTCAGGAACGTCAATGTCATGCCCTCATGTGTGCGGAGTCGTTGCCCTTATCAAATCTGCCCATCCGAATTGGTCTCCTGCAGCCATTAGATCGGCTCTCATTACAACAGCAAACACGAGGGACAAATCCTTTGACAGCATTCTAGCAGATGGATCAACGAAAGCCTCCGATCCCTTTGACATTGGTGCCGGCCATATTGACCCCATCAAAGCGATGGACCCAGGCCTCGTTTACGACATGAAAACACGCGATTACATTCTATTCCTCTGCAACATTGGCTACACCAGTGATCAAATCAATAAGATTGTTCTTTGCTCTCCCGGAACTGATACAAGCTGTCCTCAAGTGCCAATTTCAAATTCCCACATAAACTACCCCTCCATCACAGTTTCCAATCTCCGATACACCGTGACTATCAAAAGAAGCGTCCGTATCGTGGGGAAAAATAAGAAAGCGATTTATTTTTGTACTGTTTTGGAACCTGATGGAGTAGAGGTACAAATATGGCCTAGAGTTTTGATATTTTCATGGTTCAAACTAGAAAACACATATTATGTGACGCTCAAACCGCATAAGAAGTCTCAAGGGAGATATGATTTTGGAGAAATAGCGTGGTCCGATGGCTTCCACAAGGTCAGGAGTCCCTTGGTTGTATCTGTCAACACCACATGCTGTGACTCTTGTGACTCTTATGCATATTCGCATCACTCCTACAATCGTAATTTAAGCTCATTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0009566 GO:0009567 GO:0016787 GO:0017171 GO:0019538 GO:0019953 GO:0022414 GO:0043170 GO:0044238 GO:0044703 GO:0051704 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 Peptidase_S8

Protein Analysis

784

Amino Acids

86.13

Weight (kDa)

7.8

Isoelectric Point (pI)

38.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	17 - 97	7e-17	Peptidase inhibitor I9
Peptidase_S8	PF00082	126 - 580	8.1e-45	Subtilase family
fn3_6	PF17766	661 - 756	5.3e-23	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012494)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G26330 AT4G26330
fragaria_vesca	FvH4_3g45830
malus_domestica	MD03G1002600.v1.1
prunus_persica	Prupe.6G002300_v2.0.a1 Prupe.6G002300_v2.0.a1
pyrus_communis	pycom03g00250
rosa_chinensis	RchiOBHm_Chr5g0083911 RchiOBHm_Chr5g0083921
rosa_laevigata	RLG00000037064
rosa_multiflora	Rmu_sc0001137.1_g000006
rosa_roxburghii	Rroxscaffold_1G00000690
rosa_rugosa	Rorug05G0487600 Rorug05G0487600 Rorug05G0487600
rosa_samantha	Rh5AG540400 Rh5BG565400 Rh5CG587300
rosa_wichuraiana	Rw5G050240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	947, 1300
AccB1I	GGYRCC	1 cut(s)	1799
AccII	CGCG	1 cut(s)	1863
AclI	AACGTT	2 cut(s)	81, 1591
AclWI	GGATC	4 cut(s)	871, 968, 1774, 1778
AcoI	YGGCCR	1 cut(s)	1804
AcsI	RAATTY	3 cut(s)	1008, 1606, 1972
AcuI	CTGAAG	2 cut(s)	369, 813
AfaI	GTAC	3 cut(s)	640, 2083, 2112
AfiI	CCNNNNNNNGG	3 cut(s)	471, 1354, 2050
AflII	CTTAAG	1 cut(s)	325
AgsI	TTSAA	7 cut(s)	20, 119, 566, 669, 906, 1971, 2149
AhlI	ACTAGT	1 cut(s)	341
AjiI	CACGTC	1 cut(s)	102
AjnI	CCWGG	3 cut(s)	706, 1525, 1834
AjuI	GAANNNNNNNTTGG	2 cut(s)	1957, 1989
Alw21I	GWGCWC	3 cut(s)	544, 791, 1525
Alw26I	GTCTC	5 cut(s)	269, 991, 1295, 1692, 2199
AlwI	GGATC	4 cut(s)	871, 968, 1774, 1778
Ama87I	CYCGRG	1 cut(s)	193
ApeKI	GCWGC	7 cut(s)	333, 336, 809, 1019, 1256, 1544, 1694
ApoI	RAATTY	3 cut(s)	1008, 1606, 1972
ArsI	GACNNNNNNTTYG	4 cut(s)	1727, 1759, 2025, 2057
AspLEI	GCGC	2 cut(s)	730, 1450
AspS9I	GGNCC	7 cut(s)	238, 578, 968, 1226, 1478, 1831, 2229
AsuC2I	CCSGG	1 cut(s)	1935
AsuHPI	GGTGA	2 cut(s)	86, 389
AsuII	TTCGAA	1 cut(s)	268
AvaI	CYCGRG	1 cut(s)	193
AvaII	GGWCC	4 cut(s)	1226, 1478, 1831, 2229
BaeGI	GKGCMC	1 cut(s)	72
BanI	GGYRCC	1 cut(s)	1799
BanII	GRGCYC	1 cut(s)	739
BauI	CACGAG	1 cut(s)	1729
BbsI	GAAGAC	1 cut(s)	391
Bbv12I	GWGCWC	3 cut(s)	544, 791, 1525
BbvI	GCAGC	7 cut(s)	345, 348, 821, 1006, 1243, 1556, 1706
BciT130I	CCWGG	3 cut(s)	708, 1527, 1836
BciVI	GTATCC	1 cut(s)	1452
BclI	TGATCA	1 cut(s)	1903
BcnI	CCSGG	1 cut(s)	1935
BcoDI	GTCTC	5 cut(s)	269, 991, 1295, 1692, 2199
BcuI	ACTAGT	1 cut(s)	341
BfmI	CTRYAG	2 cut(s)	954, 1692
BfoI	RGCGCY	2 cut(s)	731, 1451
BfrI	CTTAAG	1 cut(s)	325
BfuAI	ACCTGC	2 cut(s)	947, 1300
BfuI	GTATCC	1 cut(s)	1452
BlpI	GCTNAGC	2 cut(s)	207, 700
Bme1390I	CCNGG	4 cut(s)	708, 1527, 1836, 1935
Bme18I	GGWCC	4 cut(s)	1226, 1478, 1831, 2229
BmeT110I	CYCGRG	1 cut(s)	193
BmgBI	CACGTC	1 cut(s)	102
BmgT120I	GGNCC	7 cut(s)	238, 578, 968, 1226, 1478, 1831, 2229
BmiI	GGNNCC	4 cut(s)	712, 1801, 1833, 2094
BmrFI	CCNGG	4 cut(s)	708, 1527, 1836, 1935
BmsI	GCATC	4 cut(s)	811, 1117, 1283, 2326
BpiI	GAAGAC	1 cut(s)	391
BpmI	CTGGAG	1 cut(s)	1509
Bpu1102I	GCTNAGC	2 cut(s)	207, 700
Bpu14I	TTCGAA	1 cut(s)	268
BpuEI	CTTGAG	7 cut(s)	207, 882, 1201, 1370, 1490, 1941, 2181
BpuMI	CCSGG	1 cut(s)	1935
Bsa29I	ATCGAT	1 cut(s)	610
BsaI	GGTCTC	2 cut(s)	991, 1692
BsaJI	CCNNGG	7 cut(s)	234, 446, 731, 1001, 1526, 1834, 2259
BsaXI	ACNNNNNCTCC	4 cut(s)	1976, 2006, 2094, 2124
Bsc4I	CCNNNNNNNGG	3 cut(s)	471, 1354, 2050
Bse118I	RCCGGY	1 cut(s)	1802
Bse1I	ACTGG	1 cut(s)	1899
Bse3DI	GCAATG	2 cut(s)	359, 930
BseBI	CCWGG	3 cut(s)	708, 1527, 1836
BseCI	ATCGAT	1 cut(s)	610
BseDI	CCNNGG	7 cut(s)	234, 446, 731, 1001, 1526, 1834, 2259
BseGI	GGATG	2 cut(s)	456, 1675
BseLI	CCNNNNNNNGG	3 cut(s)	471, 1354, 2050
BseMI	GCAATG	2 cut(s)	359, 930
BseMII	CTCAG	2 cut(s)	1290, 1347
BseNI	ACTGG	1 cut(s)	1899
BseRI	GAGGAG	3 cut(s)	507, 738, 1545
BseSI	GKGCMC	1 cut(s)	72
BseXI	GCAGC	7 cut(s)	345, 348, 821, 1006, 1243, 1556, 1706
BseYI	CCCAGC	1 cut(s)	303
BsgI	GTGCAG	1 cut(s)	1427
Bsh1236I	CGCG	1 cut(s)	1863
Bsh1285I	CGRYCG	1 cut(s)	1194
BshNI	GGYRCC	1 cut(s)	1799
BshVI	ATCGAT	1 cut(s)	610
BsiEI	CGRYCG	1 cut(s)	1194
BsiHKAI	GWGCWC	3 cut(s)	544, 791, 1525
BsiHKCI	CYCGRG	1 cut(s)	193
BsiSI	CCGG	2 cut(s)	1803, 1935
BslFI	GGGAC	5 cut(s)	320, 367, 1372, 1748, 2241
BslI	CCNNNNNNNGG	3 cut(s)	471, 1354, 2050
BsmAI	GTCTC	5 cut(s)	269, 991, 1295, 1692, 2199
BsmBI	CGTCTC	1 cut(s)	1295
BsmFI	GGGAC	5 cut(s)	320, 367, 1372, 1748, 2241
BsmI	GAATGC	2 cut(s)	1276, 1752
Bso31I	GGTCTC	2 cut(s)	991, 1692
BsoBI	CYCGRG	1 cut(s)	193
Bsp119I	TTCGAA	1 cut(s)	268
Bsp1286I	GDGCHC	5 cut(s)	72, 544, 739, 791, 1525
Bsp143I	GATC	9 cut(s)	611, 863, 973, 1033, 1284, 1703, 1766, 1783, 1903
Bsp1720I	GCTNAGC	2 cut(s)	207, 700
BspCNI	CTCAG	2 cut(s)	1291, 1346
BspDI	ATCGAT	1 cut(s)	610
BspFNI	CGCG	1 cut(s)	1863
BspLI	GGNNCC	4 cut(s)	712, 1801, 1833, 2094
BspMAI	CTGCAG	2 cut(s)	958, 1696
BspMI	ACCTGC	2 cut(s)	947, 1300
BspPI	GGATC	4 cut(s)	871, 968, 1774, 1778
BspQI	GCTCTTC	1 cut(s)	435
BspT104I	TTCGAA	1 cut(s)	268
BspT107I	GGYRCC	1 cut(s)	1799
BspTI	CTTAAG	1 cut(s)	325
BspTNI	GGTCTC	2 cut(s)	991, 1692
BsrDI	GCAATG	2 cut(s)	359, 930
BsrFI	RCCGGY	1 cut(s)	1802
BsrI	ACTGG	1 cut(s)	1899
BssAI	RCCGGY	1 cut(s)	1802
BssECI	CCNNGG	7 cut(s)	234, 446, 731, 1001, 1526, 1834, 2259
BssMI	GATC	9 cut(s)	611, 863, 973, 1033, 1284, 1703, 1766, 1783, 1903
BssSI	CACGAG	1 cut(s)	1729
BssT1I	CCWWGG	3 cut(s)	234, 1001, 2259
Bst2BI	CACGAG	1 cut(s)	1729
Bst2UI	CCWGG	3 cut(s)	708, 1527, 1836
Bst4CI	ACNGT	5 cut(s)	188, 1429, 2002, 2023, 2086
Bst6I	CTCTTC	3 cut(s)	435, 1192, 1474
BstAFI	CTTAAG	1 cut(s)	325
BstBI	TTCGAA	1 cut(s)	268
BstC8I	GCNNGC	5 cut(s)	520, 530, 544, 1160, 1804
BstDEI	CTNAG	5 cut(s)	207, 313, 700, 1299, 1333
BstDSI	CCRYGG	1 cut(s)	446
BstENI	CCTNNNNNAGG	1 cut(s)	1352
BstF5I	GGATG	2 cut(s)	456, 1675
BstFNI	CGCG	1 cut(s)	1863
BstH2I	RGCGCY	2 cut(s)	731, 1451
BstHHI	GCGC	2 cut(s)	730, 1450
BstKTI	GATC	9 cut(s)	614, 866, 976, 1036, 1287, 1706, 1769, 1786, 1906
BstMAI	GTCTC	5 cut(s)	269, 991, 1295, 1692, 2199
BstMBI	GATC	9 cut(s)	611, 863, 973, 1033, 1284, 1703, 1766, 1783, 1903
BstMCI	CGRYCG	1 cut(s)	1194
BstNI	CCWGG	3 cut(s)	708, 1527, 1836
BstNSI	RCATGY	2 cut(s)	522, 2286
BstSCI	CCNGG	4 cut(s)	706, 1525, 1834, 1933
BstSFI	CTRYAG	2 cut(s)	954, 1692
BstSLI	GKGCMC	1 cut(s)	72
BstUI	CGCG	1 cut(s)	1863
BstV1I	GCAGC	7 cut(s)	345, 348, 821, 1006, 1243, 1556, 1706
BstV2I	GAAGAC	1 cut(s)	391
BstX2I	RGATCY	1 cut(s)	973
BstXI	CCANNNNNNTGG	1 cut(s)	1170
BstYI	RGATCY	1 cut(s)	973
Bsu15I	ATCGAT	1 cut(s)	610
BsuI	GTATCC	1 cut(s)	1452
BsuTUI	ATCGAT	1 cut(s)	610
BtgI	CCRYGG	1 cut(s)	446
BtgZI	GCGATG	1 cut(s)	1841
BtrI	CACGTC	1 cut(s)	102
BtsCI	GGATG	2 cut(s)	456, 1675
BtsI	GCAGTG	1 cut(s)	666
BtsIMutI	CAGTG	3 cut(s)	267, 666, 1906
BveI	ACCTGC	2 cut(s)	947, 1300
Cac8I	GCNNGC	5 cut(s)	520, 530, 544, 1160, 1804
CfoI	GCGC	2 cut(s)	730, 1450
Cfr10I	RCCGGY	1 cut(s)	1802
Cfr13I	GGNCC	7 cut(s)	238, 578, 968, 1226, 1478, 1831, 2229
ClaI	ATCGAT	1 cut(s)	610
CseI	GACGC	3 cut(s)	843, 2029, 2182
Csp6I	GTAC	3 cut(s)	639, 2082, 2111
CspCI	CAANNNNNGTGG	4 cut(s)	642, 677, 1123, 1158
CviQI	GTAC	3 cut(s)	639, 2082, 2111
DdeI	CTNAG	5 cut(s)	207, 313, 700, 1299, 1333
DpnI	GATC	9 cut(s)	613, 865, 975, 1035, 1286, 1705, 1768, 1785, 1905
DpnII	GATC	9 cut(s)	611, 863, 973, 1033, 1284, 1703, 1766, 1783, 1903
EaeI	YGGCCR	1 cut(s)	1804
Eam1104I	CTCTTC	3 cut(s)	435, 1192, 1474
EarI	CTCTTC	3 cut(s)	435, 1192, 1474
Eco130I	CCWWGG	3 cut(s)	234, 1001, 2259
Eco147I	AGGCCT	3 cut(s)	318, 457, 1839
Eco24I	GRGCYC	1 cut(s)	739
Eco31I	GGTCTC	2 cut(s)	991, 1692
Eco32I	GATATC	3 cut(s)	258, 849, 1318
Eco47I	GGWCC	4 cut(s)	1226, 1478, 1831, 2229
Eco57I	CTGAAG	2 cut(s)	369, 813
Eco88I	CYCGRG	1 cut(s)	193
EcoNI	CCTNNNNNAGG	1 cut(s)	1352
EcoRII	CCWGG	3 cut(s)	706, 1525, 1834
EcoRV	GATATC	3 cut(s)	258, 849, 1318
EcoT14I	CCWWGG	3 cut(s)	234, 1001, 2259
EcoT22I	ATGCAT	2 cut(s)	1276, 2311
EcoT38I	GRGCYC	1 cut(s)	739
ErhI	CCWWGG	3 cut(s)	234, 1001, 2259
Esp3I	CGTCTC	1 cut(s)	1295
FalI	AAGNNNNNCTT	2 cut(s)	149, 181
FaqI	GGGAC	5 cut(s)	320, 367, 1372, 1748, 2241
FbaI	TGATCA	1 cut(s)	1903
FokI	GGATG	2 cut(s)	463, 1662
FriOI	GRGCYC	1 cut(s)	739
GlaI	GCGC	2 cut(s)	729, 1449
GsaI	CCCAGC	1 cut(s)	307
GsuI	CTGGAG	1 cut(s)	1509
HaeII	RGCGCY	2 cut(s)	731, 1451
HapII	CCGG	2 cut(s)	1803, 1935
HgaI	GACGC	3 cut(s)	843, 2029, 2182
HhaI	GCGC	2 cut(s)	730, 1450
Hin6I	GCGC	2 cut(s)	728, 1448
HinP1I	GCGC	2 cut(s)	728, 1448
HincII	GTYRAC	2 cut(s)	1345, 2275
HindII	GTYRAC	2 cut(s)	1345, 2275
HindIII	AAGCTT	2 cut(s)	900, 1090
HinfI	GANTC	7 cut(s)	355, 422, 598, 1650, 2254, 2291, 2300
HpaII	CCGG	2 cut(s)	1803, 1935
HphI	GGTGA	2 cut(s)	86, 389
Hpy166II	GTNNAC	5 cut(s)	641, 841, 1345, 1846, 2275
Hpy188I	TCNGA	7 cut(s)	427, 793, 1196, 1680, 1783, 2015, 2233
Hpy188III	TCNNGA	5 cut(s)	347, 602, 1473, 1617, 2251
Hpy8I	GTNNAC	5 cut(s)	641, 841, 1345, 1846, 2275
HpyAV	CCTTC	3 cut(s)	344, 469, 959
HpyCH4III	ACNGT	5 cut(s)	188, 1429, 2002, 2023, 2086
HpyCH4IV	ACGT	4 cut(s)	81, 101, 1591, 1622
HpyF3I	CTNAG	5 cut(s)	207, 313, 700, 1299, 1333
HpySE526I	ACGT	4 cut(s)	81, 101, 1591, 1622
HspAI	GCGC	2 cut(s)	728, 1448
KroI	GCCGGC	1 cut(s)	1802
KroNI	GCCGGC	1 cut(s)	1804
Ksp22I	TGATCA	1 cut(s)	1903
Kzo9I	GATC	9 cut(s)	611, 863, 973, 1033, 1284, 1703, 1766, 1783, 1903
LguI	GCTCTTC	1 cut(s)	435
LmnI	GCTCC	4 cut(s)	4, 725, 1003, 1528
Lsp1109I	GCAGC	7 cut(s)	345, 348, 821, 1006, 1243, 1556, 1706
LweI	GCATC	4 cut(s)	811, 1117, 1283, 2326
MaeII	ACGT	4 cut(s)	81, 101, 1591, 1622
MaeIII	GTNAC	5 cut(s)	377, 2023, 2170, 2288, 2297
MalI	GATC	9 cut(s)	613, 865, 975, 1035, 1286, 1705, 1768, 1785, 1905
MboI	GATC	9 cut(s)	611, 863, 973, 1033, 1284, 1703, 1766, 1783, 1903
MfeI	CAATTG	1 cut(s)	906
MflI	RGATCY	1 cut(s)	973
MhlI	GDGCHC	5 cut(s)	72, 544, 739, 791, 1525
MlyI	GAGTC	5 cut(s)	349, 1659, 2263, 2285, 2294
MmeI	TCCRAC	2 cut(s)	1070, 1174
Mph1103I	ATGCAT	2 cut(s)	1276, 2311
MroNI	GCCGGC	1 cut(s)	1802
MseI	TTAA	5 cut(s)	156, 326, 1110, 2339, 2350
MspA1I	CMGCKG	1 cut(s)	886
MspCI	CTTAAG	1 cut(s)	325
MspI	CCGG	2 cut(s)	1803, 1935
MspR9I	CCNGG	4 cut(s)	708, 1527, 1836, 1935
MunI	CAATTG	1 cut(s)	906
Mva1269I	GAATGC	2 cut(s)	1276, 1752
MvaI	CCWGG	3 cut(s)	708, 1527, 1836
MvnI	CGCG	1 cut(s)	1863
NaeI	GCCGGC	1 cut(s)	1804
NciI	CCSGG	1 cut(s)	1935
NdeII	GATC	9 cut(s)	611, 863, 973, 1033, 1284, 1703, 1766, 1783, 1903
NgoMIV	GCCGGC	1 cut(s)	1802
NlaIV	GGNNCC	4 cut(s)	712, 1801, 1833, 2094
NmeAIII	GCCGAG	1 cut(s)	756
NmuCI	GTSAC	5 cut(s)	377, 2023, 2170, 2288, 2297
NsiI	ATGCAT	2 cut(s)	1276, 2311
NspI	RCATGY	2 cut(s)	522, 2286
NspV	TTCGAA	1 cut(s)	268
PaeI	GCATGC	1 cut(s)	522
PceI	AGGCCT	3 cut(s)	318, 457, 1839
PciSI	GCTCTTC	1 cut(s)	435
PctI	GAATGC	2 cut(s)	1276, 1752
PdiI	GCCGGC	1 cut(s)	1804
PfeI	GAWTC	2 cut(s)	422, 598
PfoI	TCCNGGA	1 cut(s)	1933
PleI	GAGTC	5 cut(s)	349, 1658, 2262, 2285, 2294
PpsI	GAGTC	5 cut(s)	349, 1658, 2262, 2285, 2294
Psp1406I	AACGTT	2 cut(s)	81, 1591
Psp6I	CCWGG	3 cut(s)	706, 1525, 1834
PspFI	CCCAGC	1 cut(s)	303
PspGI	CCWGG	3 cut(s)	706, 1525, 1834
PspN4I	GGNNCC	4 cut(s)	712, 1801, 1833, 2094
PspPI	GGNCC	7 cut(s)	238, 578, 968, 1226, 1478, 1831, 2229
PstI	CTGCAG	2 cut(s)	958, 1696
PsuI	RGATCY	1 cut(s)	973
RsaI	GTAC	3 cut(s)	640, 2083, 2112
RsaNI	GTAC	3 cut(s)	639, 2082, 2111
SapI	GCTCTTC	1 cut(s)	435
SaqAI	TTAA	5 cut(s)	156, 326, 1110, 2339, 2350
Sau3AI	GATC	9 cut(s)	611, 863, 973, 1033, 1284, 1703, 1766, 1783, 1903
Sau96I	GGNCC	7 cut(s)	238, 578, 968, 1226, 1478, 1831, 2229
SchI	GAGTC	5 cut(s)	349, 1659, 2263, 2285, 2294
ScrFI	CCNGG	4 cut(s)	708, 1527, 1836, 1935
SduI	GDGCHC	5 cut(s)	72, 544, 739, 791, 1525
SfaNI	GCATC	4 cut(s)	811, 1117, 1283, 2326
SfcI	CTRYAG	2 cut(s)	954, 1692
SfuI	TTCGAA	1 cut(s)	268
SinI	GGWCC	4 cut(s)	1226, 1478, 1831, 2229
SmlI	CTYRAG	8 cut(s)	222, 325, 897, 1216, 1349, 1505, 1956, 2196
SmoI	CTYRAG	8 cut(s)	222, 325, 897, 1216, 1349, 1505, 1956, 2196
SpeI	ACTAGT	1 cut(s)	341
SphI	GCATGC	1 cut(s)	522
SseBI	AGGCCT	3 cut(s)	318, 457, 1839
StuI	AGGCCT	3 cut(s)	318, 457, 1839
StyD4I	CCNGG	4 cut(s)	706, 1525, 1834, 1933
StyI	CCWWGG	3 cut(s)	234, 1001, 2259
TaaI	ACNGT	5 cut(s)	188, 1429, 2002, 2023, 2086
TaiI	ACGT	4 cut(s)	84, 104, 1594, 1625
TaqI	TCGA	7 cut(s)	268, 402, 610, 816, 1036, 1320, 1472
TatI	WGTACW	1 cut(s)	2081
TauI	GCSGC	3 cut(s)	333, 874, 886
TfiI	GAWTC	2 cut(s)	422, 598
Tru1I	TTAA	5 cut(s)	156, 326, 1110, 2339, 2350
Tru9I	TTAA	5 cut(s)	156, 326, 1110, 2339, 2350
TscAI	CASTG	3 cut(s)	267, 666, 1906
TseFI	GTSAC	5 cut(s)	377, 2023, 2170, 2288, 2297
TseI	GCWGC	7 cut(s)	333, 336, 809, 1019, 1256, 1544, 1694
Tsp45I	GTSAC	5 cut(s)	377, 2023, 2170, 2288, 2297
TspDTI	ATGAA	7 cut(s)	74, 903, 908, 978, 1409, 1868, 2130
TspGWI	ACGGA	2 cut(s)	463, 2033
TspRI	CASTG	3 cut(s)	267, 666, 1906
Vha464I	CTTAAG	1 cut(s)	325
VpaK11BI	GGWCC	4 cut(s)	1226, 1478, 1831, 2229
XagI	CCTNNNNNAGG	1 cut(s)	1352
XapI	RAATTY	3 cut(s)	1008, 1606, 1972
XceI	RCATGY	2 cut(s)	522, 2286
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1825
Zsp2I	ATGCAT	2 cut(s)	1276, 2311

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.