Rorug05G0487600

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0012494
Physical Location & Seq
Forward (+)
67094393 .. 67098524
4132 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0487600.1

Sequence Viewer

Length: 1320 bp
ATGGCTGATGAATCTAGAATTAGCTTGAAGGCCTTTGTGAACAAGGGGAACAACAGAATCATGTTCGTAGAGTCTGGTTGTGATTTTGTTGAGACACTCTTCAGTTTCTTGTCAATCCCCATAGGAACTATTATCAGGCTTGCCCGTAGTCACTCAGTGCCGGTGGAAATAGGTTGCATGAACAATTTGTATGCAAGTGTTGAGAATCTTGATGTGCAGCATTTCCGGACTGAAGCTTGTAAGGACATGCTGCTATGCCCCCGCAGTCGAGCAGGAGCGACTTTGAAGAAGCTCAAATTCAACATTGAGGACCATGAGACCACGCGGTACTTTAGGTGCCCTAATGTGTTTTGCCAAAGCTCCCCAACCAAGATTAGCTATTACAGTAGTGTTCGTTGTCATTGTGGATATCTAATGGACAGAGAGATCAACCTTTCAGTTATAGTTGGTGATGAGGTGTTTGTAAAAGGACTATCCAGACTTATTATGAGTGATCATTTACAAGTCATGGCCCCAGGAACTCCAGCAATTTCTGTTTTCTTTGAGAAGCTTGGAAGCATGGATTCGAATTCTATTGAGGAAGTGACTATGAACATTGGAGTTGATGAGGTTTTGAATCTGCTTTTGTGCTCATTTGTATCAAAGACACCCCTGACTGATATACTTCTGAAGAATACTGGTAATCAACCGATATACACAATCTCTTATTCACAAATGCTAGGAGACACAATGGGTGATGGAACTAACATGTCTGTGAAGCTTATAGTTAGCAAGTCTAAGAAGGTGGTTTGTTATGCAGAAGCAGGGGAAGATTTTGTTAATTTTCTTTTCAGCTTCCTAATTTTTCCACTTGGTTTTATTTTAAAACAAATTCGGTTTGATTTAAAAGGATGTGTTGATCACTTGTACAGAAGTGTCCAGGATCTTGATGAGAAATTCTTGAAATCTCATTTCTGCAATGAACTGCTGCTTAGTCCCAAGCTTCCTCCTCGATTTTGTTATGAAAATCACCCTTTAGGAATTGGAATGGCATCATATCGTTTTGAGTCAGCTGAAATTGTGGACCCCAAATCTCACGCTAATGAAAAGGTTTGTGATCAAGGATTCTTAAAAGGGCCAGCCATGTTTATGGTAACTGACAGTCTTGCTGTAACTTCGATATCTCCCATCCTTGGGCTGTCTATTCTGAATGAAATGAATGTACCTATCACTGATATTGAGGTGAAAACAGTCTATGTGGGTAAAGAGGAGGCTTTCGGTCTTTTGGTGGCGTCTTTTGTTTCTGATCGCTCTGCCCTTACCAGTGTTGTCCTTGGTTAG

Protein Analysis

439

Amino Acids

48.87

Weight (kDa)

5.78

Isoelectric Point (pI)

35.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 4 - 416 7e-117 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012494)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26330 AT4G26330
fragaria_vesca FvH4_3g45830
malus_domestica MD03G1002600.v1.1
prunus_persica Prupe.6G002300_v2.0.a1 Prupe.6G002300_v2.0.a1
pyrus_communis pycom03g00250
rosa_chinensis RchiOBHm_Chr5g0083911 RchiOBHm_Chr5g0083921
rosa_laevigata RLG00000037064
rosa_multiflora Rmu_sc0001137.1_g000006
rosa_roxburghii Rroxscaffold_1G00000690
rosa_rugosa Rorug05G0487600 Rorug05G0487600 Rorug05G0487600
rosa_samantha Rh5AG540400 Rh5BG565400 Rh5CG587300
rosa_wichuraiana Rw5G050240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 336
AccII CGCG 1 cut(s) 325
AccIII TCCGGA 1 cut(s) 225
AciI CCGC 2 cut(s) 262, 325
AclWI GGATC 1 cut(s) 930
AcsI RAATTY 4 cut(s) 296, 568, 870, 935
AcuI CTGAAG 3 cut(s) 85, 252, 689
AcyI GRCGYC 1 cut(s) 1271
AdeI CACNNNGTG 1 cut(s) 157
AfaI GTAC 3 cut(s) 329, 908, 1203
AfiI CCNNNNNNNGG 2 cut(s) 1172, 1173
AflIII ACRYGT 1 cut(s) 747
AgsI TTSAA 5 cut(s) 28, 286, 301, 616, 943
AjnI CCWGG 2 cut(s) 514, 918
Alw21I GWGCWC 1 cut(s) 632
Alw26I GTCTC 3 cut(s) 86, 311, 717
AlwI GGATC 1 cut(s) 930
Aor13HI TCCGGA 1 cut(s) 225
AoxI GGCC 3 cut(s) 30, 510, 1115
ApeKI GCWGC 3 cut(s) 217, 250, 967
ApoI RAATTY 4 cut(s) 296, 568, 870, 935
AspS9I GGNCC 4 cut(s) 310, 511, 1063, 1115
AsuHPI GGTGA 4 cut(s) 461, 746, 1001, 1234
AsuII TTCGAA 1 cut(s) 566
AvaII GGWCC 2 cut(s) 310, 1063
BaeGI GKGCMC 1 cut(s) 341
BanI GGYRCC 1 cut(s) 336
Bbv12I GWGCWC 1 cut(s) 632
BbvI GCAGC 3 cut(s) 229, 237, 954
BccI CCATC 2 cut(s) 731, 1175
BciT130I CCWGG 2 cut(s) 516, 920
BclI TGATCA 3 cut(s) 493, 898, 1096
BcoDI GTCTC 3 cut(s) 86, 311, 717
BfaI CTAG 2 cut(s) 15, 719
BisI GCNGC 3 cut(s) 218, 251, 968
BlsI GCNGC 3 cut(s) 219, 252, 969
Bme1390I CCNGG 2 cut(s) 516, 920
Bme18I GGWCC 2 cut(s) 310, 1063
BmgT120I GGNCC 4 cut(s) 310, 511, 1063, 1115
BmiI GGNNCC 3 cut(s) 338, 513, 1065
BmrFI CCNGG 2 cut(s) 516, 920
BmsI GCATC 1 cut(s) 1040
BpmI CTGGAG 1 cut(s) 507
Bpu14I TTCGAA 1 cut(s) 566
BsaHI GRCGYC 1 cut(s) 1271
BsaI GGTCTC 1 cut(s) 311
BsaJI CCNNGG 3 cut(s) 514, 1171, 1312
BsaWI WCCGGW 1 cut(s) 225
Bsc4I CCNNNNNNNGG 2 cut(s) 1172, 1173
Bse118I RCCGGY 1 cut(s) 160
Bse1I ACTGG 2 cut(s) 682, 1302
Bse3DI GCAATG 1 cut(s) 964
BseAI TCCGGA 1 cut(s) 225
BseBI CCWGG 2 cut(s) 516, 920
BseDI CCNNGG 3 cut(s) 514, 1171, 1312
BseGI GGATG 2 cut(s) 896, 1167
BseLI CCNNNNNNNGG 2 cut(s) 1172, 1173
BseMI GCAATG 1 cut(s) 964
BseMII CTCAG 1 cut(s) 168
BseNI ACTGG 2 cut(s) 682, 1302
BseRI GAGGAG 2 cut(s) 978, 1262
BseSI GKGCMC 1 cut(s) 341
BseXI GCAGC 3 cut(s) 229, 237, 954
BsgI GTGCAG 1 cut(s) 236
Bsh1236I CGCG 1 cut(s) 325
BshFI GGCC 3 cut(s) 32, 512, 1117
BshNI GGYRCC 1 cut(s) 336
BsiHKAI GWGCWC 1 cut(s) 632
BsiSI CCGG 2 cut(s) 161, 226
BslFI GGGAC 1 cut(s) 960
BslI CCNNNNNNNGG 2 cut(s) 1172, 1173
BsmAI GTCTC 3 cut(s) 86, 311, 717
BsmFI GGGAC 1 cut(s) 960
BsnI GGCC 3 cut(s) 32, 512, 1117
Bso31I GGTCTC 1 cut(s) 311
Bsp119I TTCGAA 1 cut(s) 566
Bsp1286I GDGCHC 2 cut(s) 341, 632
Bsp13I TCCGGA 1 cut(s) 225
Bsp1407I TGTACA 1 cut(s) 906
Bsp143I GATC 6 cut(s) 426, 493, 898, 922, 1096, 1285
BspACI CCGC 2 cut(s) 262, 325
BspANI GGCC 3 cut(s) 32, 512, 1117
BspCNI CTCAG 1 cut(s) 167
BspEI TCCGGA 1 cut(s) 225
BspFNI CGCG 1 cut(s) 325
BspLI GGNNCC 3 cut(s) 338, 513, 1065
BspPI GGATC 1 cut(s) 930
BspT104I TTCGAA 1 cut(s) 566
BspT107I GGYRCC 1 cut(s) 336
BspTNI GGTCTC 1 cut(s) 311
BsrDI GCAATG 1 cut(s) 964
BsrFI RCCGGY 1 cut(s) 160
BsrGI TGTACA 1 cut(s) 906
BsrI ACTGG 2 cut(s) 682, 1302
BssAI RCCGGY 1 cut(s) 160
BssECI CCNNGG 3 cut(s) 514, 1171, 1312
BssMI GATC 6 cut(s) 426, 493, 898, 922, 1096, 1285
BssNI GRCGYC 1 cut(s) 1271
BssT1I CCWWGG 2 cut(s) 1171, 1312
Bst2UI CCWGG 2 cut(s) 516, 920
Bst4CI ACNGT 3 cut(s) 386, 1142, 1231
Bst6I CTCTTC 1 cut(s) 104
BstACI GRCGYC 1 cut(s) 1271
BstAUI TGTACA 1 cut(s) 906
BstBI TTCGAA 1 cut(s) 566
BstC8I GCNNGC 2 cut(s) 141, 1119
BstDEI CTNAG 3 cut(s) 154, 777, 971
BstF5I GGATG 2 cut(s) 896, 1167
BstFNI CGCG 1 cut(s) 325
BstKTI GATC 6 cut(s) 429, 496, 901, 925, 1099, 1288
BstMAI GTCTC 3 cut(s) 86, 311, 717
BstMBI GATC 6 cut(s) 426, 493, 898, 922, 1096, 1285
BstNI CCWGG 2 cut(s) 516, 920
BstNSI RCATGY 2 cut(s) 250, 751
BstSCI CCNGG 2 cut(s) 514, 918
BstSLI GKGCMC 1 cut(s) 341
BstUI CGCG 1 cut(s) 325
BstV1I GCAGC 3 cut(s) 229, 237, 954
BstX2I RGATCY 1 cut(s) 922
BstXI CCANNNNNNTGG 1 cut(s) 1129
BstYI RGATCY 1 cut(s) 922
BsuRI GGCC 3 cut(s) 32, 512, 1117
BtsCI GGATG 2 cut(s) 896, 1167
BtsIMutI CAGTG 3 cut(s) 162, 1209, 1309
Cac8I GCNNGC 2 cut(s) 141, 1119
Cfr10I RCCGGY 1 cut(s) 160
Cfr13I GGNCC 4 cut(s) 310, 511, 1063, 1115
CseI GACGC 1 cut(s) 1260
Csp6I GTAC 3 cut(s) 328, 907, 1202
CviAII CATG 8 cut(s) 61, 178, 247, 314, 508, 559, 748, 1123
CviQI GTAC 3 cut(s) 328, 907, 1202
DdeI CTNAG 3 cut(s) 154, 777, 971
DpnI GATC 6 cut(s) 428, 495, 900, 924, 1098, 1287
DpnII GATC 6 cut(s) 426, 493, 898, 922, 1096, 1285
DraI TTTAAA 2 cut(s) 864, 885
DraIII CACNNNGTG 1 cut(s) 157
Eam1104I CTCTTC 1 cut(s) 104
EarI CTCTTC 1 cut(s) 104
Eco130I CCWWGG 2 cut(s) 1171, 1312
Eco147I AGGCCT 1 cut(s) 32
Eco31I GGTCTC 1 cut(s) 311
Eco32I GATATC 2 cut(s) 410, 1161
Eco47I GGWCC 2 cut(s) 310, 1063
Eco57I CTGAAG 3 cut(s) 85, 252, 689
EcoRI GAATTC 1 cut(s) 568
EcoRII CCWGG 2 cut(s) 514, 918
EcoRV GATATC 2 cut(s) 410, 1161
EcoT14I CCWWGG 2 cut(s) 1171, 1312
ErhI CCWWGG 2 cut(s) 1171, 1312
FaeI CATG 8 cut(s) 64, 181, 250, 317, 511, 562, 751, 1126
FaqI GGGAC 1 cut(s) 960
FatI CATG 8 cut(s) 60, 177, 246, 313, 507, 558, 747, 1122
FauI CCCGC 1 cut(s) 269
FbaI TGATCA 3 cut(s) 493, 898, 1096
Fnu4HI GCNGC 3 cut(s) 218, 251, 968
FokI GGATG 2 cut(s) 903, 1154
Fsp4HI GCNGC 3 cut(s) 218, 251, 968
FspBI CTAG 2 cut(s) 15, 719
GluI GCNGC 3 cut(s) 218, 251, 968
GsuI CTGGAG 1 cut(s) 507
HaeIII GGCC 3 cut(s) 32, 512, 1117
HapII CCGG 2 cut(s) 161, 226
HgaI GACGC 1 cut(s) 1260
Hin1I GRCGYC 1 cut(s) 1271
Hin1II CATG 8 cut(s) 64, 181, 250, 317, 511, 562, 751, 1126
HindIII AAGCTT 4 cut(s) 234, 548, 758, 980
HinfI GANTC 8 cut(s) 11, 57, 71, 205, 563, 616, 1046, 1104
HpaII CCGG 2 cut(s) 161, 226
HphI GGTGA 4 cut(s) 461, 746, 1001, 1234
Hpy166II GTNNAC 2 cut(s) 40, 1063
Hpy188I TCNGA 3 cut(s) 669, 1188, 1285
Hpy188III TCNNGA 6 cut(s) 15, 209, 226, 477, 926, 940
Hpy8I GTNNAC 2 cut(s) 40, 1063
HpyAV CCTTC 2 cut(s) 22, 775
HpyCH4III ACNGT 3 cut(s) 386, 1142, 1231
HpyCH4V TGCA 5 cut(s) 177, 194, 217, 797, 957
HpyF3I CTNAG 3 cut(s) 154, 777, 971
Hsp92I GRCGYC 1 cut(s) 1271
Hsp92II CATG 8 cut(s) 64, 181, 250, 317, 511, 562, 751, 1126
Kpn2I TCCGGA 1 cut(s) 225
Ksp22I TGATCA 3 cut(s) 493, 898, 1096
Kzo9I GATC 6 cut(s) 426, 493, 898, 922, 1096, 1285
LmnI GCTCC 2 cut(s) 275, 365
Lsp1109I GCAGC 3 cut(s) 229, 237, 954
LweI GCATC 1 cut(s) 1040
MaeI CTAG 2 cut(s) 15, 719
MaeIII GTNAC 4 cut(s) 149, 583, 1132, 1150
MalI GATC 6 cut(s) 428, 495, 900, 924, 1098, 1287
MboI GATC 6 cut(s) 426, 493, 898, 922, 1096, 1285
MboII GAAGA 4 cut(s) 91, 298, 682, 821
MflI RGATCY 1 cut(s) 922
MhlI GDGCHC 2 cut(s) 341, 632
MlyI GAGTC 2 cut(s) 80, 1055
MnlI CCTC 9 cut(s) 301, 448, 571, 601, 996, 999, 1213, 1240, 1243
MroI TCCGGA 1 cut(s) 225
MseI TTAA 4 cut(s) 819, 863, 884, 1109
MslI CAYNNNNRTG 3 cut(s) 752, 1080, 1127
MspA1I CMGCKG 1 cut(s) 1052
MspI CCGG 2 cut(s) 161, 226
MspR9I CCNGG 2 cut(s) 516, 920
MvaI CCWGG 2 cut(s) 516, 920
MvnI CGCG 1 cut(s) 325
NdeII GATC 6 cut(s) 426, 493, 898, 922, 1096, 1285
NlaIII CATG 8 cut(s) 64, 181, 250, 317, 511, 562, 751, 1126
NlaIV GGNNCC 3 cut(s) 338, 513, 1065
NmuCI GTSAC 2 cut(s) 149, 583
NspI RCATGY 2 cut(s) 250, 751
NspV TTCGAA 1 cut(s) 566
PceI AGGCCT 1 cut(s) 32
PciI ACATGT 1 cut(s) 747
PfeI GAWTC 6 cut(s) 11, 57, 205, 563, 616, 1104
PfoI TCCNGGA 1 cut(s) 918
PkrI GCNGC 3 cut(s) 219, 252, 969
PleI GAGTC 2 cut(s) 79, 1054
PpsI GAGTC 2 cut(s) 79, 1054
PscI ACATGT 1 cut(s) 747
Psp6I CCWGG 2 cut(s) 514, 918
PspGI CCWGG 2 cut(s) 514, 918
PspN4I GGNNCC 3 cut(s) 338, 513, 1065
PspPI GGNCC 4 cut(s) 310, 511, 1063, 1115
PsuI RGATCY 1 cut(s) 922
PvuII CAGCTG 1 cut(s) 1052
RsaI GTAC 3 cut(s) 329, 908, 1203
RsaNI GTAC 3 cut(s) 328, 907, 1202
RseI CAYNNNNRTG 3 cut(s) 752, 1080, 1127
SaqAI TTAA 4 cut(s) 819, 863, 884, 1109
SatI GCNGC 3 cut(s) 218, 251, 968
Sau3AI GATC 6 cut(s) 426, 493, 898, 922, 1096, 1285
Sau96I GGNCC 4 cut(s) 310, 511, 1063, 1115
SchI GAGTC 2 cut(s) 80, 1055
ScrFI CCNGG 2 cut(s) 516, 920
SduI GDGCHC 2 cut(s) 341, 632
SfaNI GCATC 1 cut(s) 1040
SfuI TTCGAA 1 cut(s) 566
SinI GGWCC 2 cut(s) 310, 1063
SmiMI CAYNNNNRTG 3 cut(s) 752, 1080, 1127
SseBI AGGCCT 1 cut(s) 32
SsiI CCGC 2 cut(s) 262, 325
SspMI CTAG 2 cut(s) 15, 719
StuI AGGCCT 1 cut(s) 32
StyD4I CCNGG 2 cut(s) 514, 918
StyI CCWWGG 2 cut(s) 1171, 1312
TaaI ACNGT 3 cut(s) 386, 1142, 1231
TaqI TCGA 4 cut(s) 268, 566, 991, 1157
TaqII GACCGA 1 cut(s) 1247
TatI WGTACW 1 cut(s) 906
TfiI GAWTC 6 cut(s) 11, 57, 205, 563, 616, 1104
Tru1I TTAA 4 cut(s) 819, 863, 884, 1109
Tru9I TTAA 4 cut(s) 819, 863, 884, 1109
TscAI CASTG 3 cut(s) 162, 1216, 1309
TseFI GTSAC 2 cut(s) 149, 583
TseI GCWGC 3 cut(s) 217, 250, 967
Tsp45I GTSAC 2 cut(s) 149, 583
TspDTI ATGAA 8 cut(s) 24, 194, 605, 975, 1017, 1098, 1206, 1211
TspRI CASTG 3 cut(s) 162, 1216, 1309
VpaK11BI GGWCC 2 cut(s) 310, 1063
XapI RAATTY 4 cut(s) 296, 568, 870, 935
XbaI TCTAGA 1 cut(s) 14
XceI RCATGY 2 cut(s) 250, 751
XspI CTAG 2 cut(s) 15, 719
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.