RchiOBHm_Chr5g0083911

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0012494
Physical Location & Seq
Forward (+)
89580854 .. 89582541
1688 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35802

Sequence Viewer

Length: 1047 bp
ATGTTCAGCTCCTTTCCAAAGTTTTTGCCAGAGGATGTTAAACGGTCAATGCTTTATAGCTACAAGCATAGTTTCTCAGGGTTTTCGGCAAAGCTCAATTCATCACAAGCAAGCGCCTTGGCCAGGATGGAAGAAGTGATATCGGTTTTCAGAAGTAAGACACTGCAGTTGCACACAACAAGGAGTTGGGACTTCTTGGGCCTTCCCTTGATCAGCAGCAGTACTAGTACTGCAAGTGCAGCGGCCTCTCCATTGCAACTAGCATATGGCCAAGATGTCATTGTTGGGGTTTTTGATACAGGTATATGGCCTGAATCAGAAAGTTTCCGAGAAGAGCCACATATGAGGCCTATCCCCTCAACTTGGAAGGGAAAGTGTGTGAAAGGTGAGAAGTTTGAGCCAGCAAAAGCATGCAACCGGAAGCTAATTGGTGCTCGCTACTACCTGGAAGGCTTTGAAGAAGACTATGGGCCACTAAACAAGAGTGGCAATCCAGAATTTCGATCAGCTAGAGACTTTCTTGGTCACGGGACACACACAGCCTCAACAGCAGTGGGTTCAGTAGTGAAAAATAATGCAAGTTTCTATGGTTTTGCAGAGGGCACTGCCCGGGGTGGAGCACCTAAGGCTCGTCTAGCAGTGTACAAAGTTTGTTGGGGACAGAATTATAGTAGCAGGTGTACTGGAGCAGACATACTTGCAGCTTTTGATGATGCTTTGCATGATGGAGTTCATGTTATTTCAGCGTCTATTGGATCGAGACCACCACTGATACCATTGTTTGAATCAGAAGGTGCTATCGGTTCGTTTCATGCAATGCAGTTAGGTGTTAGTGTGGTGTTCTCAGCAGGTAACGAAGGGCCAGATCCATCACAGGTCACAAATGTTGCTCCTTGGGGCCTCTGTGTTGCTGCTTCATCAATTGATAGAATGTTCCCAACCAGGATTCTCCTAGATAATAAGCTCTCTATCATGGGAGAGAGCTTAATTACTAGACCTATTAAAGCAAAATTGGCAGATGCGAGCGTATTCTTTAATAATGGGTGA

Protein Analysis

348

Amino Acids

37.61

Weight (kDa)

8.51

Isoelectric Point (pI)

36.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 11 - 58 3.3e-13 Peptidase inhibitor I9
Peptidase_S8 PF00082 90 - 310 7.4e-21 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012494)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26330 AT4G26330
fragaria_vesca FvH4_3g45830
malus_domestica MD03G1002600.v1.1
prunus_persica Prupe.6G002300_v2.0.a1 Prupe.6G002300_v2.0.a1
pyrus_communis pycom03g00250
rosa_chinensis RchiOBHm_Chr5g0083911 RchiOBHm_Chr5g0083921
rosa_laevigata RLG00000037064
rosa_multiflora Rmu_sc0001137.1_g000006
rosa_roxburghii Rroxscaffold_1G00000690
rosa_rugosa Rorug05G0487600 Rorug05G0487600 Rorug05G0487600
rosa_samantha Rh5AG540400 Rh5BG565400 Rh5CG587300
rosa_wichuraiana Rw5G050240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 666
Acc36I ACCTGC 2 cut(s) 666, 839
AciI CCGC 1 cut(s) 242
AclWI GGATC 2 cut(s) 763, 860
AcoI YGGCCR 2 cut(s) 120, 268
AcsI RAATTY 1 cut(s) 497
AfaI GTAC 4 cut(s) 223, 229, 644, 682
AfiI CCNNNNNNNGG 2 cut(s) 123, 363
AgsI TTSAA 2 cut(s) 458, 785
AhlI ACTAGT 1 cut(s) 224
AjnI CCWGG 3 cut(s) 122, 444, 941
AluBI AGCT 8 cut(s) 9, 60, 94, 424, 509, 704, 964, 984
AluI AGCT 8 cut(s) 9, 60, 94, 424, 509, 704, 964, 984
Alw21I GWGCWC 2 cut(s) 436, 622
Alw26I GTCTC 2 cut(s) 507, 754
AlwI GGATC 2 cut(s) 763, 860
Ama87I CYCGRG 1 cut(s) 609
AoxI GGCC 9 cut(s) 120, 199, 243, 268, 308, 347, 470, 860, 898
ApeKI GCWGC 4 cut(s) 216, 239, 701, 911
ApoI RAATTY 1 cut(s) 497
AspLEI GCGC 1 cut(s) 116
AspS9I GGNCC 4 cut(s) 199, 470, 860, 898
AsuC2I CCSGG 2 cut(s) 610, 611
AsuHPI GGTGA 1 cut(s) 398
AvaI CYCGRG 1 cut(s) 609
AxyI CCTNAGG 1 cut(s) 624
BaeGI GKGCMC 1 cut(s) 605
BaeI ACNNNNGTAYC 2 cut(s) 764, 797
BalI TGGCCA 2 cut(s) 122, 270
BbsI GAAGAC 1 cut(s) 468
Bbv12I GWGCWC 2 cut(s) 436, 622
BbvI GCAGC 4 cut(s) 228, 251, 713, 898
BccI CCATC 3 cut(s) 121, 719, 877
BciT130I CCWGG 3 cut(s) 124, 446, 943
BclI TGATCA 1 cut(s) 210
BcnI CCSGG 2 cut(s) 610, 611
BcoDI GTCTC 2 cut(s) 507, 754
BcuI ACTAGT 1 cut(s) 224
BfaI CTAG 6 cut(s) 225, 260, 510, 635, 953, 993
BfmI CTRYAG 1 cut(s) 164
BfoI RGCGCY 1 cut(s) 117
BfuAI ACCTGC 2 cut(s) 666, 839
BisI GCNGC 5 cut(s) 217, 240, 243, 702, 912
BlsI GCNGC 5 cut(s) 218, 241, 244, 703, 913
BmcAI AGTACT 2 cut(s) 223, 229
Bme1390I CCNGG 5 cut(s) 124, 446, 610, 611, 943
BmeT110I CYCGRG 1 cut(s) 609
BmgT120I GGNCC 4 cut(s) 199, 470, 860, 898
BmiI GGNNCC 1 cut(s) 899
BmrFI CCNGG 5 cut(s) 124, 446, 610, 611, 943
BmsI GCATC 2 cut(s) 703, 1009
BpiI GAAGAC 1 cut(s) 468
BpmI CTGGAG 1 cut(s) 705
BpuMI CCSGG 2 cut(s) 610, 611
BsaI GGTCTC 1 cut(s) 754
BsaJI CCNNGG 4 cut(s) 117, 609, 610, 893
BsaWI WCCGGW 1 cut(s) 417
BsaXI ACNNNNNCTCC 2 cut(s) 720, 750
Bsc4I CCNNNNNNNGG 2 cut(s) 123, 363
Bse1I ACTGG 1 cut(s) 688
Bse21I CCTNAGG 1 cut(s) 624
Bse3DI GCAATG 2 cut(s) 251, 822
BseBI CCWGG 3 cut(s) 124, 446, 943
BseDI CCNNGG 4 cut(s) 117, 609, 610, 893
BseGI GGATG 2 cut(s) 40, 132
BseLI CCNNNNNNNGG 2 cut(s) 123, 363
BseMI GCAATG 2 cut(s) 251, 822
BseMII CTCAG 2 cut(s) 90, 858
BseNI ACTGG 1 cut(s) 688
BseSI GKGCMC 1 cut(s) 605
BseXI GCAGC 4 cut(s) 228, 251, 713, 898
BsgI GTGCAG 1 cut(s) 258
BshFI GGCC 9 cut(s) 122, 201, 245, 270, 310, 349, 472, 862, 900
BsiHKAI GWGCWC 2 cut(s) 436, 622
BsiHKCI CYCGRG 1 cut(s) 609
BsiSI CCGG 2 cut(s) 418, 610
BslFI GGGAC 3 cut(s) 203, 544, 672
BslI CCNNNNNNNGG 2 cut(s) 123, 363
BsmAI GTCTC 2 cut(s) 507, 754
BsmFI GGGAC 3 cut(s) 203, 544, 672
BsnI GGCC 9 cut(s) 122, 201, 245, 270, 310, 349, 472, 862, 900
Bso31I GGTCTC 1 cut(s) 754
BsoBI CYCGRG 1 cut(s) 609
Bsp1286I GDGCHC 3 cut(s) 436, 605, 622
Bsp1407I TGTACA 1 cut(s) 642
Bsp143I GATC 4 cut(s) 210, 503, 755, 865
BspACI CCGC 1 cut(s) 242
BspANI GGCC 9 cut(s) 122, 201, 245, 270, 310, 349, 472, 862, 900
BspCNI CTCAG 2 cut(s) 89, 857
BspLI GGNNCC 1 cut(s) 899
BspMAI CTGCAG 1 cut(s) 168
BspMI ACCTGC 2 cut(s) 666, 839
BspPI GGATC 2 cut(s) 763, 860
BspQI GCTCTTC 1 cut(s) 327
BspTNI GGTCTC 1 cut(s) 754
BsrDI GCAATG 2 cut(s) 251, 822
BsrGI TGTACA 1 cut(s) 642
BsrI ACTGG 1 cut(s) 688
BssECI CCNNGG 4 cut(s) 117, 609, 610, 893
BssMI GATC 4 cut(s) 210, 503, 755, 865
BssT1I CCWWGG 2 cut(s) 117, 893
Bst2UI CCWGG 3 cut(s) 124, 446, 943
Bst4CI ACNGT 1 cut(s) 45
Bst6I CTCTTC 1 cut(s) 327
BstAUI TGTACA 1 cut(s) 642
BstC8I GCNNGC 5 cut(s) 112, 402, 412, 436, 1024
BstDEI CTNAG 3 cut(s) 76, 624, 844
BstENI CCTNNNNNAGG 1 cut(s) 121
BstF5I GGATG 2 cut(s) 40, 132
BstH2I RGCGCY 1 cut(s) 117
BstHHI GCGC 1 cut(s) 116
BstKTI GATC 4 cut(s) 213, 506, 758, 868
BstMAI GTCTC 2 cut(s) 507, 754
BstMBI GATC 4 cut(s) 210, 503, 755, 865
BstMWI GCNNNNNNNGC 5 cut(s) 239, 548, 626, 635, 1013
BstNI CCWGG 3 cut(s) 124, 446, 943
BstNSI RCATGY 1 cut(s) 414
BstSCI CCNGG 5 cut(s) 122, 444, 608, 609, 941
BstSFI CTRYAG 1 cut(s) 164
BstSLI GKGCMC 1 cut(s) 605
BstV1I GCAGC 4 cut(s) 228, 251, 713, 898
BstV2I GAAGAC 1 cut(s) 468
BstX2I RGATCY 1 cut(s) 865
BstYI RGATCY 1 cut(s) 865
Bsu36I CCTNAGG 1 cut(s) 624
BsuRI GGCC 9 cut(s) 122, 201, 245, 270, 310, 349, 472, 862, 900
BtsCI GGATG 2 cut(s) 40, 132
BtsI GCAGTG 4 cut(s) 161, 558, 603, 645
BtsIMutI CAGTG 5 cut(s) 161, 558, 603, 645, 767
BveI ACCTGC 2 cut(s) 666, 839
Cac8I GCNNGC 5 cut(s) 112, 402, 412, 436, 1024
CfoI GCGC 1 cut(s) 116
Cfr13I GGNCC 4 cut(s) 199, 470, 860, 898
Cfr9I CCCGGG 1 cut(s) 609
CseI GACGC 1 cut(s) 735
Csp6I GTAC 4 cut(s) 222, 228, 643, 681
CspCI CAANNNNNGTGG 2 cut(s) 534, 569
CviAII CATG 5 cut(s) 411, 722, 734, 812, 973
CviQI GTAC 4 cut(s) 222, 228, 643, 681
DdeI CTNAG 3 cut(s) 76, 624, 844
DpnI GATC 4 cut(s) 212, 505, 757, 867
DpnII GATC 4 cut(s) 210, 503, 755, 865
EaeI YGGCCR 2 cut(s) 120, 268
Eam1104I CTCTTC 1 cut(s) 327
EarI CTCTTC 1 cut(s) 327
Eco130I CCWWGG 2 cut(s) 117, 893
Eco147I AGGCCT 1 cut(s) 349
Eco31I GGTCTC 1 cut(s) 754
Eco32I GATATC 1 cut(s) 141
Eco81I CCTNAGG 1 cut(s) 624
Eco88I CYCGRG 1 cut(s) 609
EcoNI CCTNNNNNAGG 1 cut(s) 121
EcoO109I RGGNCCY 1 cut(s) 898
EcoRII CCWGG 3 cut(s) 122, 444, 941
EcoRV GATATC 1 cut(s) 141
EcoT14I CCWWGG 2 cut(s) 117, 893
ErhI CCWWGG 2 cut(s) 117, 893
FaeI CATG 5 cut(s) 414, 725, 737, 815, 976
FaqI GGGAC 3 cut(s) 203, 544, 672
FatI CATG 5 cut(s) 410, 721, 733, 811, 972
FauNDI CATATG 2 cut(s) 265, 342
FbaI TGATCA 1 cut(s) 210
Fnu4HI GCNGC 5 cut(s) 217, 240, 243, 702, 912
FokI GGATG 2 cut(s) 47, 139
Fsp4HI GCNGC 5 cut(s) 217, 240, 243, 702, 912
FspBI CTAG 6 cut(s) 225, 260, 510, 635, 953, 993
GlaI GCGC 1 cut(s) 115
GluI GCNGC 5 cut(s) 217, 240, 243, 702, 912
GsuI CTGGAG 1 cut(s) 705
HaeII RGCGCY 1 cut(s) 117
HaeIII GGCC 9 cut(s) 122, 201, 245, 270, 310, 349, 472, 862, 900
HapII CCGG 2 cut(s) 418, 610
HgaI GACGC 1 cut(s) 735
HhaI GCGC 1 cut(s) 116
Hin1II CATG 5 cut(s) 414, 725, 737, 815, 976
Hin6I GCGC 1 cut(s) 114
HinP1I GCGC 1 cut(s) 114
HinfI GANTC 3 cut(s) 314, 785, 946
HpaII CCGG 2 cut(s) 418, 610
HphI GGTGA 1 cut(s) 398
Hpy166II GTNNAC 2 cut(s) 643, 681
Hpy188I TCNGA 4 cut(s) 152, 319, 329, 790
Hpy188III TCNNGA 2 cut(s) 494, 759
Hpy8I GTNNAC 2 cut(s) 643, 681
HpyAV CCTTC 5 cut(s) 212, 361, 443, 785, 851
HpyCH4III ACNGT 1 cut(s) 45
HpyF10VI GCNNNNNNNGC 5 cut(s) 239, 548, 626, 635, 1013
HpyF3I CTNAG 3 cut(s) 76, 624, 844
Hsp92II CATG 5 cut(s) 414, 725, 737, 815, 976
HspAI GCGC 1 cut(s) 114
Ksp22I TGATCA 1 cut(s) 210
Kzo9I GATC 4 cut(s) 210, 503, 755, 865
LguI GCTCTTC 1 cut(s) 327
LmnI GCTCC 4 cut(s) 14, 617, 686, 895
Lsp1109I GCAGC 4 cut(s) 228, 251, 713, 898
LweI GCATC 2 cut(s) 703, 1009
MaeI CTAG 6 cut(s) 225, 260, 510, 635, 953, 993
MaeIII GTNAC 3 cut(s) 524, 851, 877
MalI GATC 4 cut(s) 212, 505, 757, 867
MboI GATC 4 cut(s) 210, 503, 755, 865
MboII GAAGA 4 cut(s) 143, 344, 470, 473
MfeI CAATTG 1 cut(s) 921
MflI RGATCY 1 cut(s) 865
MhlI GDGCHC 3 cut(s) 436, 605, 622
MlsI TGGCCA 2 cut(s) 122, 270
MluCI AATT 7 cut(s) 97, 426, 497, 664, 921, 987, 1010
MluNI TGGCCA 2 cut(s) 122, 270
MnlI CCTC 7 cut(s) 25, 256, 339, 367, 553, 592, 911
Mox20I TGGCCA 2 cut(s) 122, 270
MscI TGGCCA 2 cut(s) 122, 270
MseI TTAA 4 cut(s) 39, 986, 1002, 1035
Msp20I TGGCCA 2 cut(s) 122, 270
MspA1I CMGCKG 1 cut(s) 242
MspI CCGG 2 cut(s) 418, 610
MspR9I CCNGG 5 cut(s) 124, 446, 610, 611, 943
MunI CAATTG 1 cut(s) 921
MvaI CCWGG 3 cut(s) 124, 446, 943
MwoI GCNNNNNNNGC 5 cut(s) 239, 548, 626, 635, 1013
NciI CCSGG 2 cut(s) 610, 611
NdeI CATATG 2 cut(s) 265, 342
NdeII GATC 4 cut(s) 210, 503, 755, 865
NlaIII CATG 5 cut(s) 414, 725, 737, 815, 976
NlaIV GGNNCC 1 cut(s) 899
NmuCI GTSAC 2 cut(s) 524, 877
NspI RCATGY 1 cut(s) 414
PaeI GCATGC 1 cut(s) 414
PaqCI CACCTGC 1 cut(s) 666
PceI AGGCCT 1 cut(s) 349
PciSI GCTCTTC 1 cut(s) 327
PfeI GAWTC 3 cut(s) 314, 785, 946
PkrI GCNGC 5 cut(s) 218, 241, 244, 703, 913
Psp6I CCWGG 3 cut(s) 122, 444, 941
PspGI CCWGG 3 cut(s) 122, 444, 941
PspN4I GGNNCC 1 cut(s) 899
PspPI GGNCC 4 cut(s) 199, 470, 860, 898
PstI CTGCAG 1 cut(s) 168
PsuI RGATCY 1 cut(s) 865
RsaI GTAC 4 cut(s) 223, 229, 644, 682
RsaNI GTAC 4 cut(s) 222, 228, 643, 681
SapI GCTCTTC 1 cut(s) 327
SaqAI TTAA 4 cut(s) 39, 986, 1002, 1035
SatI GCNGC 5 cut(s) 217, 240, 243, 702, 912
Sau3AI GATC 4 cut(s) 210, 503, 755, 865
Sau96I GGNCC 4 cut(s) 199, 470, 860, 898
ScaI AGTACT 2 cut(s) 223, 229
ScrFI CCNGG 5 cut(s) 124, 446, 610, 611, 943
SduI GDGCHC 3 cut(s) 436, 605, 622
SfaNI GCATC 2 cut(s) 703, 1009
SfcI CTRYAG 1 cut(s) 164
SmaI CCCGGG 1 cut(s) 611
SpeI ACTAGT 1 cut(s) 224
SphI GCATGC 1 cut(s) 414
Sse9I AATT 7 cut(s) 97, 426, 497, 664, 921, 987, 1010
SseBI AGGCCT 1 cut(s) 349
SsiI CCGC 1 cut(s) 242
SspMI CTAG 6 cut(s) 225, 260, 510, 635, 953, 993
StuI AGGCCT 1 cut(s) 349
StyD4I CCNGG 5 cut(s) 122, 444, 608, 609, 941
StyI CCWWGG 2 cut(s) 117, 893
TaaI ACNGT 1 cut(s) 45
TaqI TCGA 2 cut(s) 502, 758
TasI AATT 7 cut(s) 97, 426, 497, 664, 921, 987, 1010
TatI WGTACW 4 cut(s) 221, 227, 642, 680
TauI GCSGC 1 cut(s) 245
TfiI GAWTC 3 cut(s) 314, 785, 946
Tru1I TTAA 4 cut(s) 39, 986, 1002, 1035
Tru9I TTAA 4 cut(s) 39, 986, 1002, 1035
TscAI CASTG 5 cut(s) 168, 558, 610, 645, 774
TseFI GTSAC 2 cut(s) 524, 877
TseI GCWGC 4 cut(s) 216, 239, 701, 911
Tsp45I GTSAC 2 cut(s) 524, 877
TspDTI ATGAA 4 cut(s) 90, 722, 800, 906
TspMI CCCGGG 1 cut(s) 609
TspRI CASTG 5 cut(s) 168, 558, 610, 645, 774
XagI CCTNNNNNAGG 1 cut(s) 121
XapI RAATTY 1 cut(s) 497
XceI RCATGY 1 cut(s) 414
XmaI CCCGGG 1 cut(s) 609
XspI CTAG 6 cut(s) 225, 260, 510, 635, 953, 993
ZrmI AGTACT 2 cut(s) 223, 229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.