pycom03g00250

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0012494
Physical Location & Seq
Reverse (-)
229704 .. 233264
3561 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g00250.10

Sequence Viewer

Length: 2379 bp
ATGGCTAATAATAATTCTCAGTGTTTTTGGGGCCTTTTTCTTGCACTTTCACTTTATTTTACTCACTCAACTTCCACACCTCATGTTTATATAGTCTATTTGGGGCACAACTACAATGTTCATGTTCCTCACCTCACCTCAAACCATCATCTTCATCTCCTTTCCAAAGTTTTTGCAAGTGAAGAAGATGTTAAGCAATCCATGCTTTATAGCTACAAGCACAGTTTCTCGGGGTTTTCAGCTAAGCTCAATTCTTCTCAAGCAACCACCTTGGCCCAGATGGAAGAAGTGATATCAGTGTTTCGAAGTGAGACAATGCAGTTGCACACAACTAGAAGCTGGGACTTCTTAGGCCTTACCTTAAGCAGCAGCAGCACTAGTCCTGAAGGGACTCCATTGCAACAAGCCTATGGTGACGACATCATTGTTGGGGTCTTCGATACAGGTATATGGCCTGAATCCGATAGTTTCCAGGAAGAGCCACGGATGAGGCCTATCCCCTCAACTTGGAAGGGAGGGTGTGCTAGAGGAGAAATGTTTGAGCCAGAAAAAGCATGCAACCGCAAGCTAATTGGTGCTCGCTACTACCTCAAAGGTTTTGAAGAAGAATATGGGCCGCTAAACAAGAGTGGGAATCCAGAATATCGATCAGCTAGAGATTTTTTAGGTCATGGTACACACACAGCCTCAACAGCAGTGGGTTCAATAGTGAAAAACAATGCAAGTTTCTTTGGCTTAGCCTCAGGAACCGCTAGGGGAGGAGCACCGAGGGCTCGTCTAGCAGTGTATAAAGTATGTTGGGGAAAGGACTATGATGGGAGGTGCTCTGAAGCAGACATTCTTGCAGCTTTCGATGATGCTTTGCACGATGGAGTTCACGTGATATCAGCGTCGTTTGGATCAAAGCCGCCATTGTTGCCGCTGTTTATATCACAAGCTGCAATTGGTTCATTTCATGCAATGCAGTTAGGTGTTAGTGTGGTGTTCTCTGCAGGTAATGAAGGGCCAGATCCCTCACAGGTCTCAAATGTTGCTCCTTGGGGAATTTGTGTAGCTGCTTCCTCTGTTGATCGAATGTTTCCAACCCGAATAATCCTAGACAATAAGCTCTTTGTTATGGGGGAAAGCTTTATTACCACACCTATTAAGGCAAAATTAGCAGATGCAACCACATATTTTGTCAATGGGGTTTGCAGGCCAGAGAATTGGATAAAATCAAAGTTTGCGGTCGGAAGAGTAATTCTATGCTTCTCAAGCATAGGACCATACGAAATTGATGAGGCAGAAGTAGCTGCCAAGAAAGCAAATGCAACGGGTTTGATCTTTGTGGAAGCTGAGACGAGGCAGGTTGATATCGACATCATTCCCTCAGTCCATGTCAACCTTGAGCAGGGTACTAAAATCAAGCACTATCTTGCTCAATCTCCCACGAAACCTGTCGTGCAGATAGAAGCAAGTAAAACTGTCATCGGTAAGTCTCCAGCGCCTAGAGTTGCATACTTTTCTTCGAGAGGACCAAGCTCACTTGCACCTGATATTCTCAAGCCAGACGTAAGTGCTCCAGGGGTCAATATATTGGCAGCATGGCCTCCTCAAACATCTCCCACTTTGACACTTGATGATAAACGTTCTGTGAGCTGGAATTTTCAGTCAGGAACATCAATGTCATGCCCTCATGTGTGCGGAGTCGTTGCCCTTATCAAATCTGCCCATCCGAATTGGTCTCCTGCAGCCATTAGATCTGCTCTCATTACAACAGCAAACACGAGGGACACATCCCTTGACAGCATTCTAGCAGATGGATCAACGAAAGCCTCCGATCCCTTTGACATTGGTGCCGGCCATATTGACCCCATCAAAGCGATGGATCCAGGCCTCGTTTACGACATGAAAACACGCGATTACATTCTGTTCCTCTGCAACATTGGCTACACCGGTGATCAAATCAATAAGATTGTTCTTTGCTCTCCCGGAACTGATACAAGCTGTCCTCAAGTGCCAACTTCAAATTCCCACATAAACTACCCCTCCATCACAGTTTCCAATCTCCAATACACCGTGACTATCAAAAGAAGCGTCCGTAACGTGGGGAAAAATGAGAATGCGATTTATTTTTGTACCGTTTTGGAACCTGATGGAGTAGAGGTACAAATATGGCCTAGAGTTTTGATATTTTCATGGTTCAAACTAGAAAACACATATTATGTGACGCTCAAACCGCATAAGAAGTCTCAAGGGAGATATGATTTTGGAGAAATAGCGTGGTCCGATGGCTTCCACAAGGTCAGGAGTCCCTTGGTTGTATCTGTTAACACTACATGCTGCGACTCTTATGAATATTTGCATCACTCCTACAATCGCAATTTAAGCTCATTTTAA

Protein Analysis

793

Amino Acids

86.78

Weight (kDa)

6.8

Isoelectric Point (pI)

37.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012494)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26330 AT4G26330
fragaria_vesca FvH4_3g45830
malus_domestica MD03G1002600.v1.1
prunus_persica Prupe.6G002300_v2.0.a1 Prupe.6G002300_v2.0.a1
pyrus_communis pycom03g00250
rosa_chinensis RchiOBHm_Chr5g0083911 RchiOBHm_Chr5g0083921
rosa_laevigata RLG00000037064
rosa_multiflora Rmu_sc0001137.1_g000006
rosa_roxburghii Rroxscaffold_1G00000690
rosa_rugosa Rorug05G0487600 Rorug05G0487600 Rorug05G0487600
rosa_samantha Rh5AG540400 Rh5BG565400 Rh5CG587300
rosa_wichuraiana Rw5G050240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 983, 1336
AccB1I GGYRCC 1 cut(s) 1835
AccII CGCG 1 cut(s) 1899
AciI CCGC 8 cut(s) 562, 617, 750, 908, 920, 1226, 1683, 2219
AclI AACGTT 1 cut(s) 1627
AclWI GGATC 6 cut(s) 907, 1004, 1810, 1814, 1862, 1875
AcoI YGGCCR 1 cut(s) 1840
AcsI RAATTY 3 cut(s) 1044, 1642, 2008
AcuI CTGAAG 2 cut(s) 405, 849
AcvI CACGTG 1 cut(s) 880
AfaI GTAC 4 cut(s) 676, 1396, 2119, 2148
AfiI CCNNNNNNNGG 3 cut(s) 507, 1390, 2086
AflII CTTAAG 1 cut(s) 361
AgeI ACCGGT 1 cut(s) 1934
AgsI TTSAA 4 cut(s) 602, 705, 2007, 2185
AhlI ACTAGT 1 cut(s) 377
AjnI CCWGG 3 cut(s) 471, 1561, 1870
AjuI GAANNNNNNNTTGG 2 cut(s) 1993, 2025
Alw21I GWGCWC 4 cut(s) 580, 766, 827, 1561
Alw26I GTCTC 6 cut(s) 305, 1027, 1331, 1482, 1728, 2235
AlwI GGATC 6 cut(s) 907, 1004, 1810, 1814, 1862, 1875
Ama87I CYCGRG 1 cut(s) 229
ApoI RAATTY 3 cut(s) 1044, 1642, 2008
ArsI GACNNNNNNTTYG 2 cut(s) 2061, 2093
AsiGI ACCGGT 1 cut(s) 1934
AspLEI GCGC 1 cut(s) 1486
AspS9I GGNCC 7 cut(s) 31, 274, 614, 1004, 1262, 1514, 2265
AsuC2I CCSGG 1 cut(s) 1971
AsuHPI GGTGA 4 cut(s) 122, 127, 425, 1949
AsuII TTCGAA 1 cut(s) 304
AvaI CYCGRG 1 cut(s) 229
AvaII GGWCC 3 cut(s) 1262, 1514, 2265
AxyI CCTNAGG 1 cut(s) 742
BaeGI GKGCMC 1 cut(s) 108
BamHI GGATCC 1 cut(s) 1867
BanI GGYRCC 1 cut(s) 1835
BanII GRGCYC 1 cut(s) 775
BauI CACGAG 1 cut(s) 1765
BbrPI CACGTG 1 cut(s) 880
BbsI GAAGAC 1 cut(s) 427
Bbv12I GWGCWC 4 cut(s) 580, 766, 827, 1561
BciT130I CCWGG 3 cut(s) 473, 1563, 1872
BclI TGATCA 1 cut(s) 1939
BcnI CCSGG 1 cut(s) 1971
BcoDI GTCTC 6 cut(s) 305, 1027, 1331, 1482, 1728, 2235
BcuI ACTAGT 1 cut(s) 377
BfmI CTRYAG 2 cut(s) 990, 1728
BfoI RGCGCY 1 cut(s) 1487
BfrI CTTAAG 1 cut(s) 361
BfuAI ACCTGC 2 cut(s) 983, 1336
BglII AGATCT 1 cut(s) 1739
BlpI GCTNAGC 2 cut(s) 243, 736
Bme1390I CCNGG 4 cut(s) 473, 1563, 1872, 1971
Bme18I GGWCC 3 cut(s) 1262, 1514, 2265
BmeT110I CYCGRG 1 cut(s) 229
BmgT120I GGNCC 7 cut(s) 31, 274, 614, 1004, 1262, 1514, 2265
BmiI GGNNCC 5 cut(s) 32, 748, 1837, 1869, 2130
BmrFI CCNGG 4 cut(s) 473, 1563, 1872, 1971
BmsI GCATC 3 cut(s) 847, 1153, 2353
BpiI GAAGAC 1 cut(s) 427
BpmI CTGGAG 2 cut(s) 1464, 1545
Bpu1102I GCTNAGC 2 cut(s) 243, 736
Bpu14I TTCGAA 1 cut(s) 304
BpuEI CTTGAG 6 cut(s) 243, 1237, 1406, 1526, 1977, 2217
BpuMI CCSGG 1 cut(s) 1971
Bsa29I ATCGAT 1 cut(s) 646
BsaAI YACGTR 1 cut(s) 880
BsaI GGTCTC 2 cut(s) 1027, 1728
BsaJI CCNNGG 6 cut(s) 270, 482, 767, 1037, 1562, 2295
BsaWI WCCGGW 1 cut(s) 1934
BsaXI ACNNNNNCTCC 6 cut(s) 864, 894, 2012, 2042, 2130, 2160
Bsc4I CCNNNNNNNGG 3 cut(s) 507, 1390, 2086
Bse118I RCCGGY 2 cut(s) 1838, 1934
Bse21I CCTNAGG 1 cut(s) 742
Bse3DI GCAATG 2 cut(s) 395, 966
BseBI CCWGG 3 cut(s) 473, 1563, 1872
BseCI ATCGAT 1 cut(s) 646
BseDI CCNNGG 6 cut(s) 270, 482, 767, 1037, 1562, 2295
BseGI GGATG 3 cut(s) 492, 1711, 1775
BseLI CCNNNNNNNGG 3 cut(s) 507, 1390, 2086
BseMI GCAATG 2 cut(s) 395, 966
BseMII CTCAG 4 cut(s) 32, 756, 1326, 1383
BseRI GAGGAG 3 cut(s) 543, 774, 1581
BseSI GKGCMC 1 cut(s) 108
BseYI CCCAGC 1 cut(s) 339
BsgI GTGCAG 1 cut(s) 1463
Bsh1236I CGCG 1 cut(s) 1899
Bsh1285I CGRYCG 1 cut(s) 1230
BshNI GGYRCC 1 cut(s) 1835
BshTI ACCGGT 1 cut(s) 1934
BshVI ATCGAT 1 cut(s) 646
BsiEI CGRYCG 1 cut(s) 1230
BsiHKAI GWGCWC 4 cut(s) 580, 766, 827, 1561
BsiHKCI CYCGRG 1 cut(s) 229
BsiSI CCGG 3 cut(s) 1839, 1935, 1971
BslFI GGGAC 4 cut(s) 356, 403, 1784, 2277
BslI CCNNNNNNNGG 3 cut(s) 507, 1390, 2086
BsmAI GTCTC 6 cut(s) 305, 1027, 1331, 1482, 1728, 2235
BsmBI CGTCTC 1 cut(s) 1331
BsmFI GGGAC 4 cut(s) 356, 403, 1784, 2277
BsmI GAATGC 2 cut(s) 1788, 2107
Bso31I GGTCTC 2 cut(s) 1027, 1728
BsoBI CYCGRG 1 cut(s) 229
Bsp119I TTCGAA 1 cut(s) 304
Bsp1286I GDGCHC 6 cut(s) 108, 580, 766, 775, 827, 1561
Bsp1720I GCTNAGC 2 cut(s) 243, 736
BspACI CCGC 8 cut(s) 562, 617, 750, 908, 920, 1226, 1683, 2219
BspCNI CTCAG 4 cut(s) 31, 755, 1327, 1382
BspDI ATCGAT 1 cut(s) 646
BspFNI CGCG 1 cut(s) 1899
BspLI GGNNCC 5 cut(s) 32, 748, 1837, 1869, 2130
BspMAI CTGCAG 2 cut(s) 994, 1732
BspMI ACCTGC 2 cut(s) 983, 1336
BspPI GGATC 6 cut(s) 907, 1004, 1810, 1814, 1862, 1875
BspQI GCTCTTC 1 cut(s) 471
BspT104I TTCGAA 1 cut(s) 304
BspT107I GGYRCC 1 cut(s) 1835
BspTI CTTAAG 1 cut(s) 361
BspTNI GGTCTC 2 cut(s) 1027, 1728
BsrDI GCAATG 2 cut(s) 395, 966
BsrFI RCCGGY 2 cut(s) 1838, 1934
BssAI RCCGGY 2 cut(s) 1838, 1934
BssECI CCNNGG 6 cut(s) 270, 482, 767, 1037, 1562, 2295
BssSI CACGAG 1 cut(s) 1765
BssT1I CCWWGG 3 cut(s) 270, 1037, 2295
Bst2BI CACGAG 1 cut(s) 1765
Bst2UI CCWGG 3 cut(s) 473, 1563, 1872
Bst4CI ACNGT 5 cut(s) 224, 1465, 2038, 2059, 2122
Bst6I CTCTTC 2 cut(s) 471, 1228
BstAFI CTTAAG 1 cut(s) 361
BstAPI GCANNNNNTGC 1 cut(s) 202
BstBAI YACGTR 1 cut(s) 880
BstBI TTCGAA 1 cut(s) 304
BstC8I GCNNGC 5 cut(s) 556, 566, 580, 1196, 1840
BstDEI CTNAG 7 cut(s) 18, 243, 349, 736, 742, 1335, 1369
BstDSI CCRYGG 1 cut(s) 482
BstENI CCTNNNNNAGG 1 cut(s) 1388
BstF5I GGATG 3 cut(s) 492, 1711, 1775
BstFNI CGCG 1 cut(s) 1899
BstH2I RGCGCY 1 cut(s) 1487
BstHHI GCGC 1 cut(s) 1486
BstMAI GTCTC 6 cut(s) 305, 1027, 1331, 1482, 1728, 2235
BstMCI CGRYCG 1 cut(s) 1230
BstNI CCWGG 3 cut(s) 473, 1563, 1872
BstNSI RCATGY 2 cut(s) 558, 2322
BstSCI CCNGG 4 cut(s) 471, 1561, 1870, 1969
BstSFI CTRYAG 2 cut(s) 990, 1728
BstSLI GKGCMC 1 cut(s) 108
BstUI CGCG 1 cut(s) 1899
BstV2I GAAGAC 1 cut(s) 427
BstX2I RGATCY 3 cut(s) 1009, 1739, 1867
BstXI CCANNNNNNTGG 1 cut(s) 1206
BstYI RGATCY 3 cut(s) 1009, 1739, 1867
Bsu15I ATCGAT 1 cut(s) 646
Bsu36I CCTNAGG 1 cut(s) 742
BsuTUI ATCGAT 1 cut(s) 646
BtgI CCRYGG 1 cut(s) 482
BtgZI GCGATG 1 cut(s) 1877
BtsCI GGATG 3 cut(s) 492, 1711, 1775
BtsI GCAGTG 2 cut(s) 702, 789
BtsIMutI CAGTG 4 cut(s) 26, 303, 702, 789
BveI ACCTGC 2 cut(s) 983, 1336
Cac8I GCNNGC 5 cut(s) 556, 566, 580, 1196, 1840
CfoI GCGC 1 cut(s) 1486
Cfr10I RCCGGY 2 cut(s) 1838, 1934
Cfr13I GGNCC 7 cut(s) 31, 274, 614, 1004, 1262, 1514, 2265
ClaI ATCGAT 1 cut(s) 646
CseI GACGC 3 cut(s) 879, 2065, 2218
Csp6I GTAC 4 cut(s) 675, 1395, 2118, 2147
CspAI ACCGGT 1 cut(s) 1934
CspCI CAANNNNNGTGG 4 cut(s) 678, 713, 1159, 1194
CviQI GTAC 4 cut(s) 675, 1395, 2118, 2147
DdeI CTNAG 7 cut(s) 18, 243, 349, 736, 742, 1335, 1369
EaeI YGGCCR 1 cut(s) 1840
Eam1104I CTCTTC 2 cut(s) 471, 1228
EarI CTCTTC 2 cut(s) 471, 1228
Eco130I CCWWGG 3 cut(s) 270, 1037, 2295
Eco147I AGGCCT 3 cut(s) 354, 493, 1875
Eco24I GRGCYC 1 cut(s) 775
Eco31I GGTCTC 2 cut(s) 1027, 1728
Eco32I GATATC 3 cut(s) 294, 885, 1354
Eco47I GGWCC 3 cut(s) 1262, 1514, 2265
Eco57I CTGAAG 2 cut(s) 405, 849
Eco72I CACGTG 1 cut(s) 880
Eco81I CCTNAGG 1 cut(s) 742
Eco88I CYCGRG 1 cut(s) 229
EcoNI CCTNNNNNAGG 1 cut(s) 1388
EcoO109I RGGNCCY 1 cut(s) 31
EcoRII CCWGG 3 cut(s) 471, 1561, 1870
EcoRV GATATC 3 cut(s) 294, 885, 1354
EcoT14I CCWWGG 3 cut(s) 270, 1037, 2295
EcoT38I GRGCYC 1 cut(s) 775
ErhI CCWWGG 3 cut(s) 270, 1037, 2295
Esp3I CGTCTC 1 cut(s) 1331
FaqI GGGAC 4 cut(s) 356, 403, 1784, 2277
FbaI TGATCA 1 cut(s) 1939
FokI GGATG 3 cut(s) 499, 1698, 1762
FriOI GRGCYC 1 cut(s) 775
GlaI GCGC 1 cut(s) 1485
GsaI CCCAGC 1 cut(s) 343
GsuI CTGGAG 2 cut(s) 1464, 1545
HaeII RGCGCY 1 cut(s) 1487
HapII CCGG 3 cut(s) 1839, 1935, 1971
HgaI GACGC 3 cut(s) 879, 2065, 2218
HhaI GCGC 1 cut(s) 1486
Hin6I GCGC 1 cut(s) 1484
HinP1I GCGC 1 cut(s) 1484
HincII GTYRAC 2 cut(s) 1381, 2311
HindII GTYRAC 2 cut(s) 1381, 2311
HindIII AAGCTT 1 cut(s) 1126
HinfI GANTC 6 cut(s) 391, 458, 634, 1686, 2290, 2327
HpaI GTTAAC 1 cut(s) 2311
HpaII CCGG 3 cut(s) 1839, 1935, 1971
HphI GGTGA 4 cut(s) 122, 127, 425, 1949
Hpy166II GTNNAC 5 cut(s) 677, 877, 1381, 1882, 2311
Hpy188I TCNGA 6 cut(s) 463, 829, 1232, 1716, 1819, 2269
Hpy188III TCNNGA 6 cut(s) 383, 638, 744, 1509, 1653, 2287
Hpy8I GTNNAC 5 cut(s) 677, 877, 1381, 1882, 2311
Hpy99I CGWCG 1 cut(s) 895
HpyAV CCTTC 3 cut(s) 380, 505, 995
HpyCH4III ACNGT 5 cut(s) 224, 1465, 2038, 2059, 2122
HpyCH4IV ACGT 4 cut(s) 879, 1551, 1627, 2085
HpyF3I CTNAG 7 cut(s) 18, 243, 349, 736, 742, 1335, 1369
HpySE526I ACGT 4 cut(s) 879, 1551, 1627, 2085
HspAI GCGC 1 cut(s) 1484
KroI GCCGGC 1 cut(s) 1838
KroNI GCCGGC 1 cut(s) 1840
Ksp22I TGATCA 1 cut(s) 1939
KspAI GTTAAC 1 cut(s) 2311
LguI GCTCTTC 1 cut(s) 471
LmnI GCTCC 3 cut(s) 761, 1039, 1564
LweI GCATC 3 cut(s) 847, 1153, 2353
MaeII ACGT 4 cut(s) 879, 1551, 1627, 2085
MaeIII GTNAC 4 cut(s) 413, 2059, 2081, 2206
MfeI CAATTG 1 cut(s) 942
MflI RGATCY 3 cut(s) 1009, 1739, 1867
MhlI GDGCHC 6 cut(s) 108, 580, 766, 775, 827, 1561
MlyI GAGTC 4 cut(s) 385, 1695, 2299, 2321
MmeI TCCRAC 2 cut(s) 1106, 1210
MroNI GCCGGC 1 cut(s) 1838
MseI TTAA 6 cut(s) 192, 362, 1146, 2310, 2366, 2377
MspA1I CMGCKG 1 cut(s) 922
MspCI CTTAAG 1 cut(s) 361
MspI CCGG 3 cut(s) 1839, 1935, 1971
MspR9I CCNGG 4 cut(s) 473, 1563, 1872, 1971
MunI CAATTG 1 cut(s) 942
Mva1269I GAATGC 2 cut(s) 1788, 2107
MvaI CCWGG 3 cut(s) 473, 1563, 1872
MvnI CGCG 1 cut(s) 1899
NaeI GCCGGC 1 cut(s) 1840
NciI CCSGG 1 cut(s) 1971
NgoMIV GCCGGC 1 cut(s) 1838
NlaIV GGNNCC 5 cut(s) 32, 748, 1837, 1869, 2130
NmuCI GTSAC 3 cut(s) 413, 2059, 2206
NspI RCATGY 2 cut(s) 558, 2322
NspV TTCGAA 1 cut(s) 304
PaeI GCATGC 1 cut(s) 558
PceI AGGCCT 3 cut(s) 354, 493, 1875
PciSI GCTCTTC 1 cut(s) 471
PctI GAATGC 2 cut(s) 1788, 2107
PdiI GCCGGC 1 cut(s) 1840
PfeI GAWTC 2 cut(s) 458, 634
PfoI TCCNGGA 2 cut(s) 471, 1969
PinAI ACCGGT 1 cut(s) 1934
PleI GAGTC 4 cut(s) 385, 1694, 2298, 2321
PmaCI CACGTG 1 cut(s) 880
PmlI CACGTG 1 cut(s) 880
PpsI GAGTC 4 cut(s) 385, 1694, 2298, 2321
Ppu21I YACGTR 1 cut(s) 880
Psp1406I AACGTT 1 cut(s) 1627
Psp6I CCWGG 3 cut(s) 471, 1561, 1870
PspCI CACGTG 1 cut(s) 880
PspFI CCCAGC 1 cut(s) 339
PspGI CCWGG 3 cut(s) 471, 1561, 1870
PspN4I GGNNCC 5 cut(s) 32, 748, 1837, 1869, 2130
PspPI GGNCC 7 cut(s) 31, 274, 614, 1004, 1262, 1514, 2265
PstI CTGCAG 2 cut(s) 994, 1732
PsuI RGATCY 3 cut(s) 1009, 1739, 1867
RsaI GTAC 4 cut(s) 676, 1396, 2119, 2148
RsaNI GTAC 4 cut(s) 675, 1395, 2118, 2147
SapI GCTCTTC 1 cut(s) 471
SaqAI TTAA 6 cut(s) 192, 362, 1146, 2310, 2366, 2377
Sau96I GGNCC 7 cut(s) 31, 274, 614, 1004, 1262, 1514, 2265
SchI GAGTC 4 cut(s) 385, 1695, 2299, 2321
ScrFI CCNGG 4 cut(s) 473, 1563, 1872, 1971
SduI GDGCHC 6 cut(s) 108, 580, 766, 775, 827, 1561
SfaNI GCATC 3 cut(s) 847, 1153, 2353
SfcI CTRYAG 2 cut(s) 990, 1728
SfuI TTCGAA 1 cut(s) 304
SgrAI CRCCGGYG 1 cut(s) 1934
SinI GGWCC 3 cut(s) 1262, 1514, 2265
SmlI CTYRAG 7 cut(s) 258, 361, 1252, 1385, 1541, 1992, 2232
SmoI CTYRAG 7 cut(s) 258, 361, 1252, 1385, 1541, 1992, 2232
SpeI ACTAGT 1 cut(s) 377
SphI GCATGC 1 cut(s) 558
SseBI AGGCCT 3 cut(s) 354, 493, 1875
SsiI CCGC 8 cut(s) 562, 617, 750, 908, 920, 1226, 1683, 2219
SspI AATATT 1 cut(s) 2339
StuI AGGCCT 3 cut(s) 354, 493, 1875
StyD4I CCNGG 4 cut(s) 471, 1561, 1870, 1969
StyI CCWWGG 3 cut(s) 270, 1037, 2295
TaaI ACNGT 5 cut(s) 224, 1465, 2038, 2059, 2122
TaiI ACGT 4 cut(s) 882, 1554, 1630, 2088
TaqI TCGA 7 cut(s) 304, 438, 646, 852, 1072, 1356, 1508
TauI GCSGC 3 cut(s) 619, 910, 922
TfiI GAWTC 2 cut(s) 458, 634
Tru1I TTAA 6 cut(s) 192, 362, 1146, 2310, 2366, 2377
Tru9I TTAA 6 cut(s) 192, 362, 1146, 2310, 2366, 2377
TscAI CASTG 4 cut(s) 26, 303, 702, 789
TseFI GTSAC 3 cut(s) 413, 2059, 2206
Tsp45I GTSAC 3 cut(s) 413, 2059, 2206
TspDTI ATGAA 8 cut(s) 110, 143, 939, 944, 1014, 1904, 2166, 2349
TspGWI ACGGA 2 cut(s) 499, 2069
TspRI CASTG 4 cut(s) 26, 303, 702, 789
Vha464I CTTAAG 1 cut(s) 361
VpaK11BI GGWCC 3 cut(s) 1262, 1514, 2265
XagI CCTNNNNNAGG 1 cut(s) 1388
XapI RAATTY 3 cut(s) 1044, 1642, 2008
XceI RCATGY 2 cut(s) 558, 2322
XcmI CCANNNNNNNNNTGG 1 cut(s) 1861
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.