RchiOBHm_Chr5g0083921

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0012494
Physical Location & Seq
Forward (+)
89582552 .. 89584189
1638 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35803

Sequence Viewer

Length: 1188 bp
ATGACTTGCTATAGTATGGTTTGCACGTCCAGCAATTGGAAAGAACCAAAATCTGCTGAAGGTAGAGTAATCCTATGCTTCTCAGCCATAGGACCGTATGATATTGGTGACGCTGAAGTGGCTGCCAAGCTAGCCAATGCATCAGCTTTGATATTTGTAGAACCAATGTCCAGGGAGATTGGTGCTGACATCATCCCCTCAGTCCATGTCAACCTTGAGCAAGGGACTCGGATCTTGAACTATCTTACTGACTCTCCCACGAAGCCTGTTGTGCAGATAGAAGCAAGTAAAACTATTATCGGGAAGTCACTGGCCCCTAGAGTCGCATACTTCTCTTCAAGAGGCCCAAGCTCACTTACACCTGATATTCTCAAGCCAGATATAAGTGCTCCAGGGATGAACATATTAGCAGCATGGCCTAATCAAACTTCTCCAACTTTGAGACGTGATGATAACCGTTCAGTTAACTGGAATTTTCAGTCAGGAACATCAATGTCCTGTCCTCATGTCTCCGGAGTTGTTGCTCTCATCAAATCTGCACACCCAAATTGGTCTCCTGCAGCCATTAAATCTGCTATCATGACCACAGCATACACAAAGGACACGTCATTTGACACCATTCTAGCCGATGGACCCATGAAAGCCTCCGACCCTTTTGACTTAGGTGCCGGTCACATAGACCCCATCAAGGCAATGGATCCGGGGCTTGTCTACGACATGAAAACAAGGGACTATGTTCAATTCCTATGCAACATTGGCTATACTGAAGAACAGATAAACATGATTGTTCTTTGCCCTTCAGGAACTGACACAAGCTGTCCACGAGTGCCCAAATCAAATGCCAATGTAAACTACCCATCAATCACAGTTTCAAATCTCCAGTTCGCTGTGACAATCAAAAGAACCATGCGTAATGTGGGCGAGAACAAGAACGCCATTTATTTTGGGACCATTTCTGAGCCTGATGGAGTGGAGGTAGTGATATGGCCTAGAGTTTTGGTATTCTCCTGGTTCAAGGCGGAAAACACATATTATGTGACGCTCAAACCACAAAAGAAGTCACAAGGAAGATATGATTTTGGAGAGATCGTTTGGTCTGATGGCTTCCACAGGGTCAGGAGTCCTTTGGTTGTTTGTGTCAACACTACTCAGGATTCCAATGCTTCCATGAACTTAAGCTACATGTGA

Protein Analysis

395

Amino Acids

43.31

Weight (kDa)

6.94

Isoelectric Point (pI)

41.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 105 - 202 4.4e-22 Subtilase family
fn3_6 PF17766 282 - 378 2.1e-25 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012494)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26330 AT4G26330
fragaria_vesca FvH4_3g45830
malus_domestica MD03G1002600.v1.1
prunus_persica Prupe.6G002300_v2.0.a1 Prupe.6G002300_v2.0.a1
pyrus_communis pycom03g00250
rosa_chinensis RchiOBHm_Chr5g0083911 RchiOBHm_Chr5g0083921
rosa_laevigata RLG00000037064
rosa_multiflora Rmu_sc0001137.1_g000006
rosa_roxburghii Rroxscaffold_1G00000690
rosa_rugosa Rorug05G0487600 Rorug05G0487600 Rorug05G0487600
rosa_samantha Rh5AG540400 Rh5BG565400 Rh5CG587300
rosa_wichuraiana Rw5G050240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 665
AccB7I CCANNNNNTGG 1 cut(s) 36
AccI GTMKAC 1 cut(s) 711
AccIII TCCGGA 1 cut(s) 512
AciI CCGC 1 cut(s) 1019
AclWI GGATC 3 cut(s) 239, 692, 705
AcsI RAATTY 1 cut(s) 472
AcuI CTGAAG 4 cut(s) 78, 135, 783, 786
AfiI CCNNNNNNNGG 2 cut(s) 36, 688
AflII CTTAAG 1 cut(s) 1174
AflIII ACRYGT 2 cut(s) 603, 1182
AgsI TTSAA 5 cut(s) 238, 339, 740, 873, 1015
AjiI CACGTC 3 cut(s) 27, 446, 606
AjnI CCWGG 3 cut(s) 170, 391, 1007
AluBI AGCT 5 cut(s) 130, 146, 351, 816, 1179
AluI AGCT 5 cut(s) 130, 146, 351, 816, 1179
Alw21I GWGCWC 1 cut(s) 391
Alw26I GTCTC 3 cut(s) 436, 514, 558
AlwI GGATC 3 cut(s) 239, 692, 705
AlwNI CAGNNNCTG 1 cut(s) 806
Aor13HI TCCGGA 1 cut(s) 512
AoxI GGCC 4 cut(s) 312, 343, 416, 986
ApeKI GCWGC 3 cut(s) 122, 410, 560
ApoI RAATTY 1 cut(s) 472
ArsI GACNNNNNNTTYG 4 cut(s) 590, 593, 622, 625
AspS9I GGNCC 5 cut(s) 92, 313, 344, 632, 948
AsuC2I CCSGG 1 cut(s) 702
AsuHPI GGTGA 1 cut(s) 119
AsuNHI GCTAGC 1 cut(s) 130
AvaII GGWCC 3 cut(s) 92, 632, 948
BaeGI GKGCMC 1 cut(s) 831
BamHI GGATCC 1 cut(s) 697
BanI GGYRCC 1 cut(s) 665
BauI CACGAG 1 cut(s) 822
Bbv12I GWGCWC 1 cut(s) 391
BbvI GCAGC 3 cut(s) 109, 422, 572
BccI CCATC 5 cut(s) 623, 692, 865, 959, 1094
BcgI CGANNNNNNTGC 2 cut(s) 209, 243
BciT130I CCWGG 3 cut(s) 172, 393, 1009
BcnI CCSGG 1 cut(s) 702
BcoDI GTCTC 3 cut(s) 436, 514, 558
BfaI CTAG 4 cut(s) 131, 318, 623, 990
BfmI CTRYAG 2 cut(s) 10, 558
BfrI CTTAAG 1 cut(s) 1174
BisI GCNGC 3 cut(s) 123, 411, 561
BlsI GCNGC 3 cut(s) 124, 412, 562
Bme1390I CCNGG 4 cut(s) 172, 393, 702, 1009
Bme18I GGWCC 3 cut(s) 92, 632, 948
BmgBI CACGTC 3 cut(s) 27, 446, 606
BmgT120I GGNCC 5 cut(s) 92, 313, 344, 632, 948
BmiI GGNNCC 5 cut(s) 315, 634, 667, 699, 949
BmrFI CCNGG 4 cut(s) 172, 393, 702, 1009
BmsI GCATC 1 cut(s) 149
BmtI GCTAGC 1 cut(s) 134
BpmI CTGGAG 2 cut(s) 375, 863
BpuEI CTTGAG 2 cut(s) 236, 356
BpuMI CCSGG 1 cut(s) 702
BsaI GGTCTC 1 cut(s) 558
BsaJI CCNNGG 3 cut(s) 171, 392, 701
BsaWI WCCGGW 1 cut(s) 512
BsaXI ACNNNNNCTCC 6 cut(s) 238, 268, 960, 990, 1074, 1104
Bsc4I CCNNNNNNNGG 2 cut(s) 36, 688
Bse118I RCCGGY 1 cut(s) 668
Bse1I ACTGG 3 cut(s) 315, 473, 880
Bse3DI GCAATG 1 cut(s) 699
BseAI TCCGGA 1 cut(s) 512
BseBI CCWGG 3 cut(s) 172, 393, 1009
BseDI CCNNGG 3 cut(s) 171, 392, 701
BseGI GGATG 2 cut(s) 192, 402
BseLI CCNNNNNNNGG 2 cut(s) 36, 688
BseMI GCAATG 1 cut(s) 699
BseMII CTCAG 4 cut(s) 96, 213, 948, 1163
BseNI ACTGG 3 cut(s) 315, 473, 880
BseSI GKGCMC 1 cut(s) 831
BseXI GCAGC 3 cut(s) 109, 422, 572
BsgI GTGCAG 2 cut(s) 293, 522
BshFI GGCC 4 cut(s) 314, 345, 418, 988
BshNI GGYRCC 1 cut(s) 665
BsiHKAI GWGCWC 1 cut(s) 391
BsiSI CCGG 3 cut(s) 513, 669, 701
BslFI GGGAC 3 cut(s) 238, 743, 961
BslI CCNNNNNNNGG 2 cut(s) 36, 688
BsmAI GTCTC 3 cut(s) 436, 514, 558
BsmBI CGTCTC 1 cut(s) 436
BsmFI GGGAC 3 cut(s) 238, 743, 961
BsnI GGCC 4 cut(s) 314, 345, 418, 988
Bso31I GGTCTC 1 cut(s) 558
Bsp1286I GDGCHC 2 cut(s) 391, 831
Bsp13I TCCGGA 1 cut(s) 512
Bsp143I GATC 3 cut(s) 231, 697, 1086
BspACI CCGC 1 cut(s) 1019
BspANI GGCC 4 cut(s) 314, 345, 418, 988
BspCNI CTCAG 4 cut(s) 95, 212, 949, 1162
BspEI TCCGGA 1 cut(s) 512
BspHI TCATGA 1 cut(s) 579
BspLI GGNNCC 5 cut(s) 315, 634, 667, 699, 949
BspMAI CTGCAG 1 cut(s) 562
BspOI GCTAGC 1 cut(s) 134
BspPI GGATC 3 cut(s) 239, 692, 705
BspT107I GGYRCC 1 cut(s) 665
BspTI CTTAAG 1 cut(s) 1174
BspTNI GGTCTC 1 cut(s) 558
BsrDI GCAATG 1 cut(s) 699
BsrFI RCCGGY 1 cut(s) 668
BsrI ACTGG 3 cut(s) 315, 473, 880
BssAI RCCGGY 1 cut(s) 668
BssECI CCNNGG 3 cut(s) 171, 392, 701
BssMI GATC 3 cut(s) 231, 697, 1086
BssSI CACGAG 1 cut(s) 822
Bst2BI CACGAG 1 cut(s) 822
Bst2UI CCWGG 3 cut(s) 172, 393, 1009
Bst4CI ACNGT 3 cut(s) 96, 458, 868
Bst6I CTCTTC 1 cut(s) 340
BstAFI CTTAAG 1 cut(s) 1174
BstC8I GCNNGC 1 cut(s) 132
BstDEI CTNAG 5 cut(s) 82, 199, 661, 957, 1149
BstF5I GGATG 2 cut(s) 192, 402
BstKTI GATC 3 cut(s) 234, 700, 1089
BstMAI GTCTC 3 cut(s) 436, 514, 558
BstMBI GATC 3 cut(s) 231, 697, 1086
BstMWI GCNNNNNNNGC 5 cut(s) 30, 119, 131, 271, 756
BstNI CCWGG 3 cut(s) 172, 393, 1009
BstNSI RCATGY 1 cut(s) 1186
BstSCI CCNGG 4 cut(s) 170, 391, 700, 1007
BstSFI CTRYAG 2 cut(s) 10, 558
BstSLI GKGCMC 1 cut(s) 831
BstV1I GCAGC 3 cut(s) 109, 422, 572
BstX2I RGATCY 2 cut(s) 231, 697
BstYI RGATCY 2 cut(s) 231, 697
BsuRI GGCC 4 cut(s) 314, 345, 418, 988
BtrI CACGTC 3 cut(s) 27, 446, 606
BtsCI GGATG 2 cut(s) 192, 402
BtsIMutI CAGTG 1 cut(s) 308
Cac8I GCNNGC 1 cut(s) 132
CaiI CAGNNNCTG 1 cut(s) 806
CciI TCATGA 1 cut(s) 579
Cfr10I RCCGGY 1 cut(s) 668
Cfr13I GGNCC 5 cut(s) 92, 313, 344, 632, 948
CseI GACGC 2 cut(s) 119, 1048
DdeI CTNAG 5 cut(s) 82, 199, 661, 957, 1149
DpnI GATC 3 cut(s) 233, 699, 1088
DpnII GATC 3 cut(s) 231, 697, 1086
Eam1104I CTCTTC 1 cut(s) 340
EarI CTCTTC 1 cut(s) 340
EciI GGCGGA 1 cut(s) 1034
Eco31I GGTCTC 1 cut(s) 558
Eco47I GGWCC 3 cut(s) 92, 632, 948
Eco57I CTGAAG 4 cut(s) 78, 135, 783, 786
EcoRII CCWGG 3 cut(s) 170, 391, 1007
EcoT22I ATGCAT 1 cut(s) 142
Esp3I CGTCTC 1 cut(s) 436
FaqI GGGAC 3 cut(s) 238, 743, 961
FblI GTMKAC 1 cut(s) 711
Fnu4HI GCNGC 3 cut(s) 123, 411, 561
FokI GGATG 2 cut(s) 179, 409
Fsp4HI GCNGC 3 cut(s) 123, 411, 561
FspBI CTAG 4 cut(s) 131, 318, 623, 990
GluI GCNGC 3 cut(s) 123, 411, 561
GsuI CTGGAG 2 cut(s) 375, 863
HaeIII GGCC 4 cut(s) 314, 345, 418, 988
HapII CCGG 3 cut(s) 513, 669, 701
HgaI GACGC 2 cut(s) 119, 1048
HincII GTYRAC 3 cut(s) 211, 466, 1141
HindII GTYRAC 3 cut(s) 211, 466, 1141
HinfI GANTC 5 cut(s) 226, 251, 321, 1120, 1154
HpaI GTTAAC 1 cut(s) 466
HpaII CCGG 3 cut(s) 513, 669, 701
HphI GGTGA 1 cut(s) 119
Hpy166II GTNNAC 6 cut(s) 211, 466, 712, 821, 850, 1141
Hpy188I TCNGA 4 cut(s) 231, 649, 958, 1099
Hpy188III TCNNGA 9 cut(s) 235, 301, 339, 483, 513, 580, 801, 1117, 1151
Hpy8I GTNNAC 6 cut(s) 211, 466, 712, 821, 850, 1141
HpyAV CCTTC 2 cut(s) 53, 807
HpyCH4III ACNGT 3 cut(s) 96, 458, 868
HpyCH4IV ACGT 3 cut(s) 26, 445, 605
HpyCH4V TGCA 6 cut(s) 24, 140, 274, 539, 560, 750
HpyF10VI GCNNNNNNNGC 5 cut(s) 30, 119, 131, 271, 756
HpyF3I CTNAG 5 cut(s) 82, 199, 661, 957, 1149
HpySE526I ACGT 3 cut(s) 26, 445, 605
Kpn2I TCCGGA 1 cut(s) 512
KspAI GTTAAC 1 cut(s) 466
Kzo9I GATC 3 cut(s) 231, 697, 1086
LmnI GCTCC 1 cut(s) 394
Lsp1109I GCAGC 3 cut(s) 109, 422, 572
LweI GCATC 1 cut(s) 149
MaeI CTAG 4 cut(s) 131, 318, 623, 990
MaeII ACGT 3 cut(s) 26, 445, 605
MaeIII GTNAC 6 cut(s) 107, 306, 671, 889, 1036, 1059
MalI GATC 3 cut(s) 233, 699, 1088
MboI GATC 3 cut(s) 231, 697, 1086
MboII GAAGA 3 cut(s) 327, 779, 1080
MfeI CAATTG 1 cut(s) 34
MflI RGATCY 2 cut(s) 231, 697
MhlI GDGCHC 2 cut(s) 391, 831
MluCI AATT 4 cut(s) 34, 472, 547, 740
MlyI GAGTC 4 cut(s) 220, 245, 330, 1129
MmeI TCCRAC 2 cut(s) 458, 672
MnlI CCTC 5 cut(s) 208, 335, 513, 655, 967
Mph1103I ATGCAT 1 cut(s) 142
MroI TCCGGA 1 cut(s) 512
MseI TTAA 3 cut(s) 465, 567, 1175
MspCI CTTAAG 1 cut(s) 1174
MspI CCGG 3 cut(s) 513, 669, 701
MspR9I CCNGG 4 cut(s) 172, 393, 702, 1009
MunI CAATTG 1 cut(s) 34
MvaI CCWGG 3 cut(s) 172, 393, 1009
MwoI GCNNNNNNNGC 5 cut(s) 30, 119, 131, 271, 756
NciI CCSGG 1 cut(s) 702
NdeII GATC 3 cut(s) 231, 697, 1086
NheI GCTAGC 1 cut(s) 130
NlaIV GGNNCC 5 cut(s) 315, 634, 667, 699, 949
NmuCI GTSAC 6 cut(s) 107, 306, 671, 889, 1036, 1059
NsiI ATGCAT 1 cut(s) 142
NspI RCATGY 1 cut(s) 1186
PagI TCATGA 1 cut(s) 579
PciI ACATGT 1 cut(s) 1182
PfeI GAWTC 1 cut(s) 1154
PflMI CCANNNNNTGG 1 cut(s) 36
PkrI GCNGC 3 cut(s) 124, 412, 562
PleI GAGTC 4 cut(s) 220, 245, 329, 1128
PpsI GAGTC 4 cut(s) 220, 245, 329, 1128
PscI ACATGT 1 cut(s) 1182
Psp6I CCWGG 3 cut(s) 170, 391, 1007
PspGI CCWGG 3 cut(s) 170, 391, 1007
PspN4I GGNNCC 5 cut(s) 315, 634, 667, 699, 949
PspPI GGNCC 5 cut(s) 92, 313, 344, 632, 948
PsrI GAACNNNNNNTAC 1 cut(s) 1163
PstI CTGCAG 1 cut(s) 562
PstNI CAGNNNCTG 1 cut(s) 806
PsuI RGATCY 2 cut(s) 231, 697
SaqAI TTAA 3 cut(s) 465, 567, 1175
SatI GCNGC 3 cut(s) 123, 411, 561
Sau3AI GATC 3 cut(s) 231, 697, 1086
Sau96I GGNCC 5 cut(s) 92, 313, 344, 632, 948
SchI GAGTC 4 cut(s) 220, 245, 330, 1129
ScrFI CCNGG 4 cut(s) 172, 393, 702, 1009
SduI GDGCHC 2 cut(s) 391, 831
SfaNI GCATC 1 cut(s) 149
SfcI CTRYAG 2 cut(s) 10, 558
SinI GGWCC 3 cut(s) 92, 632, 948
SmlI CTYRAG 3 cut(s) 215, 371, 1174
SmoI CTYRAG 3 cut(s) 215, 371, 1174
Sse9I AATT 4 cut(s) 34, 472, 547, 740
SsiI CCGC 1 cut(s) 1019
SspMI CTAG 4 cut(s) 131, 318, 623, 990
StyD4I CCNGG 4 cut(s) 170, 391, 700, 1007
TaaI ACNGT 3 cut(s) 96, 458, 868
TaiI ACGT 3 cut(s) 29, 448, 608
TasI AATT 4 cut(s) 34, 472, 547, 740
TfiI GAWTC 1 cut(s) 1154
Tru1I TTAA 3 cut(s) 465, 567, 1175
Tru9I TTAA 3 cut(s) 465, 567, 1175
TscAI CASTG 1 cut(s) 315
TseFI GTSAC 6 cut(s) 107, 306, 671, 889, 1036, 1059
TseI GCWGC 3 cut(s) 122, 410, 560
Tsp45I GTSAC 6 cut(s) 107, 306, 671, 889, 1036, 1059
TspDTI ATGAA 4 cut(s) 413, 653, 734, 1184
TspRI CASTG 1 cut(s) 315
Van91I CCANNNNNTGG 1 cut(s) 36
Vha464I CTTAAG 1 cut(s) 1174
VpaK11BI GGWCC 3 cut(s) 92, 632, 948
XapI RAATTY 1 cut(s) 472
XceI RCATGY 1 cut(s) 1186
XcmI CCANNNNNNNNNTGG 2 cut(s) 691, 913
XmiI GTMKAC 1 cut(s) 711
XspI CTAG 4 cut(s) 131, 318, 623, 990
Zsp2I ATGCAT 1 cut(s) 142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.