Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0012494

Physical Location & Seq

Genomic Coordinates Reverse (-)

1135156 .. 1138897

Gene Structure 3742 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00000690.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2364 bp

ATGGCTAGTGATTCTCAGTGTTTTTGGGGCCTTTGCCTCTCACTTTCACTATATTTTATTCACTCAAATTCAACACCTCATGTGTACATAGTCTATTTGGGGCTCAGTCATAATATTCATGAACCTCACCTGACTTCAGAACACCATATTCAGCTCCTTTCCAAAGTTTTTGCCAGTGAAGAGGATGTTAAACGGTCAATGCTTTATAGCTACAAGCATAGTTTCTCAGGGTTTTCAGCAAAGCTCAATTCATCACAAGCAAGCGCCTTGGCCAAGATGGAAGAAGTGATATCGGTTTTCAGAAGTAAGACACTGCAGTTGCACACAACAAGGAGTTGGGACTTCTTGGGTCTTCCCTTGATCAGCAGCAGTACTAGTACTGCAAGTGCAGCAACCTCTCCATTGCAACTAGCATATGGGCAAGACGTCATTGTTGGGGTTTTTGATACAGGTATATGGCCTGAATCAGAAAGTTTCCGAGAAGAGCCACATATGAGGCCTATCCCCTCAACTTGGAAGGGAAAGTGTGTGAAAGGTGAGATGTTTGAGCCAGCAAAAGCATGCAACCGGAAGCTAATTGGTGCTCGATACTACCTGGAAGGCTTTGAAGAAGACTATGGGCCACTAAACACGAGTGGCAATCCAGAATTTCGATCAGCTAGAGACTTTCTTGGTCACGGGACACACACAGCCTCAACAGCAGTGGGTTCAGTAGTGAAAAACAATGCAAGTTTCTATGGTTTTGCAGAGGGCACTGCCCGGGGTGGAGCACCTAAGGCTCGTCTAGCAGTGTACAAAGTTTGTTGGGGACAGAATTATAGTAGCAGGTGTACTGGAGCAGACATACTTGCAGCTTTTGATGATGCTTTGCATGATGGAGTTCATGTTATTTCAGCGTCTATTGGATCGAGACCACCACTGATACCATTGTTTGAATCAGAAGGTGCTATCGGTTCGTTTCATGCAATGCAGTTAGGTGTTAGTGTGGTGTTCTCAGCAGGTAACGAAGGGCCAGATCCATCACAGGTCACAAATGTTGCTCCTTGGAGCCTCTGTGTTGCTGCTTCATCAATTGATAGAATGTTCCCAACCAGGATTCTCCTAGATAATAAGCTCTCTATCATGGGAGAGAGCTTAATTACTACACCTATTAAAGCAAAATTGGCAGATGCGAGCGTATTCTTTAATAATGGGGTTTGCACGTCCAGCAATTGGAAAGAACCAAAATCTGCTGAAGGTAGAGTAATCCTATGCTTCTCAGCCATAGGACCGTATGATATTGGTGAGGCTGAAGTGGCTGCCAAGCTAGCCAAAGCATCAGCTTTGATATTTGTAGAACCAATGTCCAGGGAGATTGGTGTTGACATCATCCCCTCAGTCCATGTCAACCTCGAGCAGGGGACACGGATCTTGAACTATCTTACTGAATCTCCCACGAAGCCTGTTGTGCAGATAGAAGCAAGTAAAACTATTATCGGGAAGTCACCAGCCCCTAGAGTCGCATACTTCTCTTCAAGAGGCCCAAGTTCGCTTACACCTGATATTCTCAAGCCAGATATAAGTGCTCCAGGGATGAACATATTAGCAGCATGGCCTAATCAAACTTCTCCAACTTTGAGACGTGATGATAACCGTTCAGTTCACTGGAATTTTCAGTCAGGAACATCAATGTCCTGTCCTCATGTCTCCGGAGTTGTTGCTCTCATCAAATCTGCACACCCAAATTGGTCTCCTGCAGCCATTAAATCTGCTATCATGACCACAGCATACACAAAGGACACGTCATTTGACACCATTCTAGCCGATGGACCCATGAAAGCCTCCGACCCTTTTGACTTAGGTGCTGGTCACATAGACCCCATCAAGGCAATGGATCCGGGGCTTGTCTACGACATGAAAACAAGTGACTATGTTCAATTCCTATGCAACATTGGCTATACTGAAGAACAGATAAACATGATTGTTCTTTGCCCTTCAGGAACTGACACAAGCTGTCCACGAGTGCACAAATCAAATGCCTATGTAAACTACCCATCAATCACAGTTTCAAATCTCCAGTCCGCTGTGACAATCAAAAGAACCATGCGTAATGTGGGCGAGAACAAGAACGCCATTTATTTTGGGACCATTTCTGAGCCTGATGGAGTGGAGGTAGTGATATGGCCTAGAGTTTTGGTATTCTCCTGGTTCAAGGCGGAAAACACATACTATGTGACGCTCAAACCACAAAAGAAGTCACAAGGAAGATATGATTTTGGAGAGATCGTTTGGTCTGATGGCTTCCACAGGGTCAGGAGTCCTTTGGTTGTTTGTGTCAACACTACTCAGGATTCCAATGCTTCCATGAACTTAAGCTACATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0009566 GO:0009567 GO:0016787 GO:0017171 GO:0019538 GO:0019953 GO:0022414 GO:0043170 GO:0044238 GO:0044703 GO:0051704 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 Peptidase_S8

Protein Analysis

787

Amino Acids

85.81

Weight (kDa)

6.63

Isoelectric Point (pI)

41.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	28 - 108	4.5e-17	Peptidase inhibitor I9
Peptidase_S8	PF00082	140 - 594	5.8e-46	Subtilase family
fn3_6	PF17766	675 - 770	7e-25	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012494)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G26330 AT4G26330
fragaria_vesca	FvH4_3g45830
malus_domestica	MD03G1002600.v1.1
prunus_persica	Prupe.6G002300_v2.0.a1 Prupe.6G002300_v2.0.a1
pyrus_communis	pycom03g00250
rosa_chinensis	RchiOBHm_Chr5g0083911 RchiOBHm_Chr5g0083921
rosa_laevigata	RLG00000037064
rosa_multiflora	Rmu_sc0001137.1_g000006
rosa_roxburghii	Rroxscaffold_1G00000690
rosa_rugosa	Rorug05G0487600 Rorug05G0487600 Rorug05G0487600
rosa_samantha	Rh5AG540400 Rh5BG565400 Rh5CG587300
rosa_wichuraiana	Rw5G050240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	816
AatII	GACGTC	1 cut(s)	429
Acc36I	ACCTGC	2 cut(s)	816, 989
AccB7I	CCANNNNNTGG	1 cut(s)	1212
AccI	GTMKAC	1 cut(s)	1887
AccIII	TCCGGA	1 cut(s)	1688
AciI	CCGC	2 cut(s)	2061, 2195
AclWI	GGATC	5 cut(s)	913, 1010, 1415, 1868, 1881
AcoI	YGGCCR	1 cut(s)	270
AcsI	RAATTY	3 cut(s)	67, 647, 1648
AcuI	CTGAAG	5 cut(s)	120, 1254, 1311, 1959, 1962
AcyI	GRCGYC	1 cut(s)	426
AfaI	GTAC	5 cut(s)	86, 373, 379, 794, 832
AfiI	CCNNNNNNNGG	4 cut(s)	513, 1212, 1396, 1864
AflII	CTTAAG	1 cut(s)	2350
AflIII	ACRYGT	2 cut(s)	1779, 2358
AgsI	TTSAA	8 cut(s)	72, 608, 935, 1414, 1515, 1916, 2049, 2191
AhlI	ACTAGT	1 cut(s)	374
AjiI	CACGTC	3 cut(s)	1203, 1622, 1782
AjnI	CCWGG	5 cut(s)	594, 1091, 1346, 1567, 2183
Alw21I	GWGCWC	4 cut(s)	586, 772, 1567, 2007
Alw26I	GTCTC	5 cut(s)	657, 904, 1612, 1690, 1734
Alw44I	GTGCAC	1 cut(s)	2003
AlwI	GGATC	5 cut(s)	913, 1010, 1415, 1868, 1881
AlwNI	CAGNNNCTG	1 cut(s)	1982
Ama87I	CYCGRG	2 cut(s)	759, 1391
Aor13HI	TCCGGA	1 cut(s)	1688
AoxI	GGCC	9 cut(s)	28, 270, 458, 497, 620, 1010, 1519, 1592, 2162
ApaLI	GTGCAC	1 cut(s)	2003
ApeKI	GCWGC	7 cut(s)	366, 389, 851, 1061, 1298, 1586, 1736
ApoI	RAATTY	3 cut(s)	67, 647, 1648
ArsI	GACNNNNNNTTYG	6 cut(s)	1766, 1769, 1798, 1801, 2042, 2074
AspLEI	GCGC	1 cut(s)	266
AspS9I	GGNCC	7 cut(s)	28, 620, 1010, 1268, 1520, 1808, 2124
AsuC2I	CCSGG	3 cut(s)	760, 761, 1878
AsuHPI	GGTGA	4 cut(s)	119, 548, 1295, 1476
AsuNHI	GCTAGC	1 cut(s)	1306
AvaI	CYCGRG	2 cut(s)	759, 1391
AvaII	GGWCC	3 cut(s)	1268, 1808, 2124
AxyI	CCTNAGG	1 cut(s)	774
BaeGI	GKGCMC	2 cut(s)	755, 2007
BaeI	ACNNNNGTAYC	2 cut(s)	914, 947
BalI	TGGCCA	1 cut(s)	272
BamHI	GGATCC	1 cut(s)	1873
BanII	GRGCYC	1 cut(s)	105
BauI	CACGAG	2 cut(s)	631, 1998
BbsI	GAAGAC	2 cut(s)	344, 618
Bbv12I	GWGCWC	4 cut(s)	586, 772, 1567, 2007
BbvI	GCAGC	7 cut(s)	378, 401, 863, 1048, 1285, 1598, 1748
BccI	CCATC	8 cut(s)	271, 869, 1027, 1799, 1868, 2041, 2135, 2270
BciT130I	CCWGG	5 cut(s)	596, 1093, 1348, 1569, 2185
BclI	TGATCA	1 cut(s)	360
BcnI	CCSGG	3 cut(s)	760, 761, 1878
BcoDI	GTCTC	5 cut(s)	657, 904, 1612, 1690, 1734
BcuI	ACTAGT	1 cut(s)	374
BfmI	CTRYAG	2 cut(s)	314, 1734
BfoI	RGCGCY	1 cut(s)	267
BfrI	CTTAAG	1 cut(s)	2350
BfuAI	ACCTGC	2 cut(s)	816, 989
BisI	GCNGC	7 cut(s)	367, 390, 852, 1062, 1299, 1587, 1737
BlsI	GCNGC	7 cut(s)	368, 391, 853, 1063, 1300, 1588, 1738
BmcAI	AGTACT	2 cut(s)	373, 379
Bme1390I	CCNGG	8 cut(s)	596, 760, 761, 1093, 1348, 1569, 1878, 2185
Bme18I	GGWCC	3 cut(s)	1268, 1808, 2124
BmeT110I	CYCGRG	2 cut(s)	759, 1391
BmgBI	CACGTC	3 cut(s)	1203, 1622, 1782
BmgT120I	GGNCC	7 cut(s)	28, 620, 1010, 1268, 1520, 1808, 2124
BmiI	GGNNCC	5 cut(s)	29, 1049, 1810, 1875, 2125
BmrFI	CCNGG	8 cut(s)	596, 760, 761, 1093, 1348, 1569, 1878, 2185
BmsI	GCATC	3 cut(s)	853, 1159, 1325
BmtI	GCTAGC	1 cut(s)	1310
BpiI	GAAGAC	2 cut(s)	344, 618
BpmI	CTGGAG	3 cut(s)	855, 1551, 2039
BpuEI	CTTGAG	1 cut(s)	1532
BpuMI	CCSGG	3 cut(s)	760, 761, 1878
BsaHI	GRCGYC	1 cut(s)	426
BsaI	GGTCTC	2 cut(s)	904, 1734
BsaJI	CCNNGG	7 cut(s)	267, 759, 760, 1043, 1347, 1568, 1877
BsaWI	WCCGGW	2 cut(s)	567, 1688
Bsc4I	CCNNNNNNNGG	4 cut(s)	513, 1212, 1396, 1864
Bse1I	ACTGG	4 cut(s)	174, 838, 1649, 2056
Bse21I	CCTNAGG	1 cut(s)	774
Bse3DI	GCAATG	3 cut(s)	401, 972, 1875
BseAI	TCCGGA	1 cut(s)	1688
BseBI	CCWGG	5 cut(s)	596, 1093, 1348, 1569, 2185
BseDI	CCNNGG	7 cut(s)	267, 759, 760, 1043, 1347, 1568, 1877
BseGI	GGATG	3 cut(s)	190, 1368, 1578
BseLI	CCNNNNNNNGG	4 cut(s)	513, 1212, 1396, 1864
BseMI	GCAATG	3 cut(s)	401, 972, 1875
BseMII	CTCAG	8 cut(s)	29, 118, 240, 1008, 1272, 1389, 2124, 2339
BseNI	ACTGG	4 cut(s)	174, 838, 1649, 2056
BseSI	GKGCMC	2 cut(s)	755, 2007
BseXI	GCAGC	7 cut(s)	378, 401, 863, 1048, 1285, 1598, 1748
BsgI	GTGCAG	3 cut(s)	408, 1469, 1698
BshFI	GGCC	9 cut(s)	30, 272, 460, 499, 622, 1012, 1521, 1594, 2164
BsiHKAI	GWGCWC	4 cut(s)	586, 772, 1567, 2007
BsiHKCI	CYCGRG	2 cut(s)	759, 1391
BsiSI	CCGG	4 cut(s)	568, 760, 1689, 1877
BslFI	GGGAC	5 cut(s)	353, 694, 822, 1414, 2137
BslI	CCNNNNNNNGG	4 cut(s)	513, 1212, 1396, 1864
BsmAI	GTCTC	5 cut(s)	657, 904, 1612, 1690, 1734
BsmBI	CGTCTC	1 cut(s)	1612
BsmFI	GGGAC	5 cut(s)	353, 694, 822, 1414, 2137
BsnI	GGCC	9 cut(s)	30, 272, 460, 499, 622, 1012, 1521, 1594, 2164
Bso31I	GGTCTC	2 cut(s)	904, 1734
BsoBI	CYCGRG	2 cut(s)	759, 1391
Bsp1286I	GDGCHC	6 cut(s)	105, 586, 755, 772, 1567, 2007
Bsp13I	TCCGGA	1 cut(s)	1688
Bsp1407I	TGTACA	2 cut(s)	84, 792
Bsp143I	GATC	7 cut(s)	360, 653, 905, 1015, 1407, 1873, 2262
BspACI	CCGC	2 cut(s)	2061, 2195
BspANI	GGCC	9 cut(s)	30, 272, 460, 499, 622, 1012, 1521, 1594, 2164
BspCNI	CTCAG	8 cut(s)	28, 117, 239, 1007, 1271, 1388, 2125, 2338
BspEI	TCCGGA	1 cut(s)	1688
BspHI	TCATGA	2 cut(s)	118, 1755
BspLI	GGNNCC	5 cut(s)	29, 1049, 1810, 1875, 2125
BspMAI	CTGCAG	2 cut(s)	318, 1738
BspMI	ACCTGC	2 cut(s)	816, 989
BspOI	GCTAGC	1 cut(s)	1310
BspPI	GGATC	5 cut(s)	913, 1010, 1415, 1868, 1881
BspQI	GCTCTTC	1 cut(s)	477
BspTI	CTTAAG	1 cut(s)	2350
BspTNI	GGTCTC	2 cut(s)	904, 1734
BsrDI	GCAATG	3 cut(s)	401, 972, 1875
BsrGI	TGTACA	2 cut(s)	84, 792
BsrI	ACTGG	4 cut(s)	174, 838, 1649, 2056
BssECI	CCNNGG	7 cut(s)	267, 759, 760, 1043, 1347, 1568, 1877
BssMI	GATC	7 cut(s)	360, 653, 905, 1015, 1407, 1873, 2262
BssNI	GRCGYC	1 cut(s)	426
BssSI	CACGAG	2 cut(s)	631, 1998
BssT1I	CCWWGG	2 cut(s)	267, 1043
Bst2BI	CACGAG	2 cut(s)	631, 1998
Bst2UI	CCWGG	5 cut(s)	596, 1093, 1348, 1569, 2185
Bst4CI	ACNGT	4 cut(s)	195, 1272, 1634, 2044
Bst6I	CTCTTC	3 cut(s)	174, 477, 1516
BstACI	GRCGYC	1 cut(s)	426
BstAFI	CTTAAG	1 cut(s)	2350
BstAUI	TGTACA	2 cut(s)	84, 792
BstC8I	GCNNGC	5 cut(s)	262, 552, 562, 1174, 1308
BstENI	CCTNNNNNAGG	1 cut(s)	1394
BstF5I	GGATG	3 cut(s)	190, 1368, 1578
BstH2I	RGCGCY	1 cut(s)	267
BstHHI	GCGC	1 cut(s)	266
BstKTI	GATC	7 cut(s)	363, 656, 908, 1018, 1410, 1876, 2265
BstMAI	GTCTC	5 cut(s)	657, 904, 1612, 1690, 1734
BstMBI	GATC	7 cut(s)	360, 653, 905, 1015, 1407, 1873, 2262
BstNI	CCWGG	5 cut(s)	596, 1093, 1348, 1569, 2185
BstNSI	RCATGY	2 cut(s)	564, 2362
BstSCI	CCNGG	8 cut(s)	594, 758, 759, 1091, 1346, 1567, 1876, 2183
BstSFI	CTRYAG	2 cut(s)	314, 1734
BstSLI	GKGCMC	2 cut(s)	755, 2007
BstV1I	GCAGC	7 cut(s)	378, 401, 863, 1048, 1285, 1598, 1748
BstV2I	GAAGAC	2 cut(s)	344, 618
BstX2I	RGATCY	3 cut(s)	1015, 1407, 1873
BstYI	RGATCY	3 cut(s)	1015, 1407, 1873
Bsu36I	CCTNAGG	1 cut(s)	774
BsuRI	GGCC	9 cut(s)	30, 272, 460, 499, 622, 1012, 1521, 1594, 2164
BtrI	CACGTC	3 cut(s)	1203, 1622, 1782
BtsCI	GGATG	3 cut(s)	190, 1368, 1578
BtsI	GCAGTG	4 cut(s)	311, 708, 753, 795
BtsIMutI	CAGTG	8 cut(s)	23, 181, 311, 708, 753, 795, 917, 1642
BveI	ACCTGC	2 cut(s)	816, 989
Cac8I	GCNNGC	5 cut(s)	262, 552, 562, 1174, 1308
CaiI	CAGNNNCTG	1 cut(s)	1982
CciI	TCATGA	2 cut(s)	118, 1755
CfoI	GCGC	1 cut(s)	266
Cfr13I	GGNCC	7 cut(s)	28, 620, 1010, 1268, 1520, 1808, 2124
Cfr9I	CCCGGG	1 cut(s)	759
CseI	GACGC	2 cut(s)	885, 2224
Csp6I	GTAC	5 cut(s)	85, 372, 378, 793, 831
CspCI	CAANNNNNGTGG	2 cut(s)	684, 719
CviQI	GTAC	5 cut(s)	85, 372, 378, 793, 831
DpnI	GATC	7 cut(s)	362, 655, 907, 1017, 1409, 1875, 2264
DpnII	GATC	7 cut(s)	360, 653, 905, 1015, 1407, 1873, 2262
EaeI	YGGCCR	1 cut(s)	270
Eam1104I	CTCTTC	3 cut(s)	174, 477, 1516
EarI	CTCTTC	3 cut(s)	174, 477, 1516
EciI	GGCGGA	1 cut(s)	2210
Eco130I	CCWWGG	2 cut(s)	267, 1043
Eco147I	AGGCCT	1 cut(s)	499
Eco24I	GRGCYC	1 cut(s)	105
Eco31I	GGTCTC	2 cut(s)	904, 1734
Eco32I	GATATC	1 cut(s)	291
Eco47I	GGWCC	3 cut(s)	1268, 1808, 2124
Eco57I	CTGAAG	5 cut(s)	120, 1254, 1311, 1959, 1962
Eco81I	CCTNAGG	1 cut(s)	774
Eco88I	CYCGRG	2 cut(s)	759, 1391
EcoNI	CCTNNNNNAGG	1 cut(s)	1394
EcoO109I	RGGNCCY	1 cut(s)	28
EcoRII	CCWGG	5 cut(s)	594, 1091, 1346, 1567, 2183
EcoRV	GATATC	1 cut(s)	291
EcoT14I	CCWWGG	2 cut(s)	267, 1043
EcoT38I	GRGCYC	1 cut(s)	105
ErhI	CCWWGG	2 cut(s)	267, 1043
Esp3I	CGTCTC	1 cut(s)	1612
FaqI	GGGAC	5 cut(s)	353, 694, 822, 1414, 2137
FauNDI	CATATG	2 cut(s)	415, 492
FbaI	TGATCA	1 cut(s)	360
FblI	GTMKAC	1 cut(s)	1887
Fnu4HI	GCNGC	7 cut(s)	367, 390, 852, 1062, 1299, 1587, 1737
FokI	GGATG	3 cut(s)	197, 1355, 1585
FriOI	GRGCYC	1 cut(s)	105
Fsp4HI	GCNGC	7 cut(s)	367, 390, 852, 1062, 1299, 1587, 1737
GlaI	GCGC	1 cut(s)	265
GluI	GCNGC	7 cut(s)	367, 390, 852, 1062, 1299, 1587, 1737
GsuI	CTGGAG	3 cut(s)	855, 1551, 2039
HaeII	RGCGCY	1 cut(s)	267
HaeIII	GGCC	9 cut(s)	30, 272, 460, 499, 622, 1012, 1521, 1594, 2164
HapII	CCGG	4 cut(s)	568, 760, 1689, 1877
HgaI	GACGC	2 cut(s)	885, 2224
HhaI	GCGC	1 cut(s)	266
Hin1I	GRCGYC	1 cut(s)	426
Hin6I	GCGC	1 cut(s)	264
HinP1I	GCGC	1 cut(s)	264
HincII	GTYRAC	3 cut(s)	1363, 1387, 2317
HindII	GTYRAC	3 cut(s)	1363, 1387, 2317
HinfI	GANTC	8 cut(s)	11, 464, 935, 1096, 1427, 1497, 2296, 2330
HpaII	CCGG	4 cut(s)	568, 760, 1689, 1877
HphI	GGTGA	4 cut(s)	119, 548, 1295, 1476
Hpy188I	TCNGA	8 cut(s)	139, 302, 469, 479, 940, 1825, 2134, 2275
HpyAV	CCTTC	6 cut(s)	511, 593, 935, 1001, 1229, 1983
HpyCH4III	ACNGT	4 cut(s)	195, 1272, 1634, 2044
HpyCH4IV	ACGT	4 cut(s)	426, 1202, 1621, 1781
HpySE526I	ACGT	4 cut(s)	426, 1202, 1621, 1781
Hsp92I	GRCGYC	1 cut(s)	426
HspAI	GCGC	1 cut(s)	264
Kpn2I	TCCGGA	1 cut(s)	1688
Ksp22I	TGATCA	1 cut(s)	360
Kzo9I	GATC	7 cut(s)	360, 653, 905, 1015, 1407, 1873, 2262
LguI	GCTCTTC	1 cut(s)	477
LmnI	GCTCC	6 cut(s)	159, 767, 836, 1045, 1047, 1570
Lsp1109I	GCAGC	7 cut(s)	378, 401, 863, 1048, 1285, 1598, 1748
LweI	GCATC	3 cut(s)	853, 1159, 1325
MaeII	ACGT	4 cut(s)	426, 1202, 1621, 1781
MaeIII	GTNAC	9 cut(s)	674, 1001, 1027, 1482, 1847, 1904, 2065, 2212, 2235
MalI	GATC	7 cut(s)	362, 655, 907, 1017, 1409, 1875, 2264
MboI	GATC	7 cut(s)	360, 653, 905, 1015, 1407, 1873, 2262
MboII	GAAGA	9 cut(s)	191, 293, 344, 494, 620, 623, 1503, 1955, 2256
MfeI	CAATTG	2 cut(s)	1071, 1210
MflI	RGATCY	3 cut(s)	1015, 1407, 1873
MhlI	GDGCHC	6 cut(s)	105, 586, 755, 772, 1567, 2007
MlsI	TGGCCA	1 cut(s)	272
MluNI	TGGCCA	1 cut(s)	272
MlyI	GAGTC	2 cut(s)	1506, 2305
MmeI	TCCRAC	2 cut(s)	1634, 1848
Mox20I	TGGCCA	1 cut(s)	272
MroI	TCCGGA	1 cut(s)	1688
MscI	TGGCCA	1 cut(s)	272
MseI	TTAA	6 cut(s)	189, 1136, 1152, 1185, 1743, 2351
Msp20I	TGGCCA	1 cut(s)	272
MspA1I	CMGCKG	1 cut(s)	2063
MspCI	CTTAAG	1 cut(s)	2350
MspI	CCGG	4 cut(s)	568, 760, 1689, 1877
MspR9I	CCNGG	8 cut(s)	596, 760, 761, 1093, 1348, 1569, 1878, 2185
MunI	CAATTG	2 cut(s)	1071, 1210
MvaI	CCWGG	5 cut(s)	596, 1093, 1348, 1569, 2185
NciI	CCSGG	3 cut(s)	760, 761, 1878
NdeI	CATATG	2 cut(s)	415, 492
NdeII	GATC	7 cut(s)	360, 653, 905, 1015, 1407, 1873, 2262
NheI	GCTAGC	1 cut(s)	1306
NlaIV	GGNNCC	5 cut(s)	29, 1049, 1810, 1875, 2125
NmuCI	GTSAC	8 cut(s)	674, 1027, 1482, 1847, 1904, 2065, 2212, 2235
NspI	RCATGY	2 cut(s)	564, 2362
PaeI	GCATGC	1 cut(s)	564
PaeR7I	CTCGAG	1 cut(s)	1391
PagI	TCATGA	2 cut(s)	118, 1755
PaqCI	CACCTGC	1 cut(s)	816
PceI	AGGCCT	1 cut(s)	499
PciI	ACATGT	1 cut(s)	2358
PciSI	GCTCTTC	1 cut(s)	477
PfeI	GAWTC	6 cut(s)	11, 464, 935, 1096, 1427, 2330
PflMI	CCANNNNNTGG	1 cut(s)	1212
PkrI	GCNGC	7 cut(s)	368, 391, 853, 1063, 1300, 1588, 1738
PleI	GAGTC	2 cut(s)	1505, 2304
PpsI	GAGTC	2 cut(s)	1505, 2304
PscI	ACATGT	1 cut(s)	2358
Psp6I	CCWGG	5 cut(s)	594, 1091, 1346, 1567, 2183
PspGI	CCWGG	5 cut(s)	594, 1091, 1346, 1567, 2183
PspN4I	GGNNCC	5 cut(s)	29, 1049, 1810, 1875, 2125
PspPI	GGNCC	7 cut(s)	28, 620, 1010, 1268, 1520, 1808, 2124
PspXI	VCTCGAGB	1 cut(s)	1391
PsrI	GAACNNNNNNTAC	1 cut(s)	2339
PstI	CTGCAG	2 cut(s)	318, 1738
PstNI	CAGNNNCTG	1 cut(s)	1982
PsuI	RGATCY	3 cut(s)	1015, 1407, 1873
RsaI	GTAC	5 cut(s)	86, 373, 379, 794, 832
RsaNI	GTAC	5 cut(s)	85, 372, 378, 793, 831
SapI	GCTCTTC	1 cut(s)	477
SaqAI	TTAA	6 cut(s)	189, 1136, 1152, 1185, 1743, 2351
SatI	GCNGC	7 cut(s)	367, 390, 852, 1062, 1299, 1587, 1737
Sau3AI	GATC	7 cut(s)	360, 653, 905, 1015, 1407, 1873, 2262
Sau96I	GGNCC	7 cut(s)	28, 620, 1010, 1268, 1520, 1808, 2124
ScaI	AGTACT	2 cut(s)	373, 379
SchI	GAGTC	2 cut(s)	1506, 2305
ScrFI	CCNGG	8 cut(s)	596, 760, 761, 1093, 1348, 1569, 1878, 2185
SduI	GDGCHC	6 cut(s)	105, 586, 755, 772, 1567, 2007
SfaNI	GCATC	3 cut(s)	853, 1159, 1325
SfcI	CTRYAG	2 cut(s)	314, 1734
Sfr274I	CTCGAG	1 cut(s)	1391
SinI	GGWCC	3 cut(s)	1268, 1808, 2124
SlaI	CTCGAG	1 cut(s)	1391
SmaI	CCCGGG	1 cut(s)	761
SmlI	CTYRAG	3 cut(s)	1391, 1547, 2350
SmoI	CTYRAG	3 cut(s)	1391, 1547, 2350
SpeI	ACTAGT	1 cut(s)	374
SphI	GCATGC	1 cut(s)	564
SseBI	AGGCCT	1 cut(s)	499
SsiI	CCGC	2 cut(s)	2061, 2195
SspI	AATATT	1 cut(s)	115
StuI	AGGCCT	1 cut(s)	499
StyD4I	CCNGG	8 cut(s)	594, 758, 759, 1091, 1346, 1567, 1876, 2183
StyI	CCWWGG	2 cut(s)	267, 1043
TaaI	ACNGT	4 cut(s)	195, 1272, 1634, 2044
TaiI	ACGT	4 cut(s)	429, 1205, 1624, 1784
TaqI	TCGA	4 cut(s)	586, 652, 908, 1392
TatI	WGTACW	5 cut(s)	84, 371, 377, 792, 830
TfiI	GAWTC	6 cut(s)	11, 464, 935, 1096, 1427, 2330
Tru1I	TTAA	6 cut(s)	189, 1136, 1152, 1185, 1743, 2351
Tru9I	TTAA	6 cut(s)	189, 1136, 1152, 1185, 1743, 2351
TscAI	CASTG	8 cut(s)	23, 181, 318, 708, 760, 795, 924, 1649
TseFI	GTSAC	8 cut(s)	674, 1027, 1482, 1847, 1904, 2065, 2212, 2235
TseI	GCWGC	7 cut(s)	366, 389, 851, 1061, 1298, 1586, 1736
Tsp45I	GTSAC	8 cut(s)	674, 1027, 1482, 1847, 1904, 2065, 2212, 2235
TspGWI	ACGGA	1 cut(s)	1420
TspMI	CCCGGG	1 cut(s)	759
TspRI	CASTG	8 cut(s)	23, 181, 318, 708, 760, 795, 924, 1649
Van91I	CCANNNNNTGG	1 cut(s)	1212
Vha464I	CTTAAG	1 cut(s)	2350
VneI	GTGCAC	1 cut(s)	2003
VpaK11BI	GGWCC	3 cut(s)	1268, 1808, 2124
XagI	CCTNNNNNAGG	1 cut(s)	1394
XapI	RAATTY	3 cut(s)	67, 647, 1648
XceI	RCATGY	2 cut(s)	564, 2362
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1867, 2089
XhoI	CTCGAG	1 cut(s)	1391
XmaI	CCCGGG	1 cut(s)	759
XmiI	GTMKAC	1 cut(s)	1887
ZraI	GACGTC	1 cut(s)	427
ZrmI	AGTACT	2 cut(s)	373, 379

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.