Rh5BG565400

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0012494
Physical Location & Seq
Forward (+)
89524458 .. 89531905
7448 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG565400.1

Sequence Viewer

Length: 2364 bp
ATGGCTAGTGATTCTCAGTGTTTTTGGGGCCTTTGCCTCTCACTTTCACTATATTTTATTCACTCAAATTCGACACCTCATGTGTACATAGTCTATTTGGGGCTCACTCATAATATTCATGAACCTCAGCTGACTTCAGAACACCATGTTCAGCTCCTTTCCAAAGTTTTTGCCAGTGAAGAGGATGTTAAACGGTCAATGCTTTATAGCTACAAGCATACTTTCTCAGGGTTTTCGGCAAAGCTCAATTCATCACAAGCAAGCGCCTTGGCCAAGATGGAAGAAGTGATATCGGTTTTCAGAAGTAAGACACTGCAGTTGCACACAACAAGGAGTTGGGACTTCTTGGGCCTTCCCTTGATCAGCAGCAGTACTAGTACTGCAAGTGCAGCGGCCTCTCCATTGCAATTAGCATATGGCCAAGACGTCATTGTTGGGGTTTTTGATACAGGTATATGGCCTGAATCAGAAAGTTTCCGAGAAGAGCCACATATGAGGCCTATCCCCTCAACTTGGAAGGGAAAGTGTGTGAAAGGTGAGAAGTTTGAGCCAGCAAAAGCATGCAACCGGAAGCTAATTGGTGCTCGCTACTACCTGGAAGGCTTTGAAGAAGACTATGGGCCACTAAACAAGAGTGGCAATCCAGAATTTCGATCAGCTAGAGACTTTCTTGGTCACGGGACACACACAGCCTCAACAGCAGTGGGTTCAGTAGTGAAAAACAATGCAAGTTTCTATGGTTTTGCGGAGGGCACTGCCCGGGGTGGAGCACCTAAGGCTCGTCTAGCAGTGTACAAAGTTTGTTGGGGACAGAATTATAGTAGCAGGTGTACTGGAGCAGACATACTTGCAGCTTTTGATGATGCTTTGCATGATGGAGTTCATGTTATTTCAGCGTCTATTGGATCGAGACCACCACTGATACCATTGTTTGAATCAGAAGGTGCTATCGGTTCGTTTCATGCAATGCAGTTAGGTGTTAGTGTGGTGTTCTCAGCAGGTAACGAAGGGCCAGATCCATCACAGGTCACAAATGTTGCTCCTTGGAGCCTCTGTGTTGCTGCTTCATCAATTGATAGAATGTTCCCAACCAGGATTCTCCTAGATAATAAGCTCTCTATCATGGGAGAGAGCTTAATTACTACACCTATTAAAGCAAAATTGGCAGATGCGAGCGTATTCTTTAATAATGGGGTTTGCACGTCCAGCAATTGGAAAGAACCAAAATCTGCTCAAGGTAGAGTAATCCTATGCTTCTCAGCCATAGGACCGTATGATATTGGTGAGGCTGAAGTGGCTGCCAAGCTAGCCAATGCATCAGCTTTGATATTTGTAGAACCCATGTCCAGGGAGATTGGTGCTGACATCATCCCCTCAGTCCATGTCAACCTTGAGCAAGGGACTCGGATCTTGAACTATCTTACTGAATCTCCCACGAAGCCTGTTGTGCAGATAGAAGCAAGTAAAACTATTATCGGGAAGTCACTGGCCCCTAGAGTCGCATACTTCTCTTCAAGAGGCCCAAGCTCACTTACACCTGATATTCTCAAGCCAGATATAAGTGCTCCAGGGATGAACATATTAGCGGCATGGCCTAATCAAACTTCTCCAACTTTGAGACGTGATGATACCCGTTCAGTTAACTGGAATTTTCAGTCAGGAACATCAATGTCCTGTCCTCATGTCTCCGGAGTTGTTGCTCTCATCAAATCTGCACACCCAAATTGGTCTCCTGCAGCCATTAAATCTGCTATCATGACCACAGCATACACAAAGGACACGTCATTTGACACCATTCTAGCCGATGGACCCATGAAAGCCTCCGACCCTTTTGACTTAGGTGCCGGTCACATAGACCCCATCAAGGCAATGGATCCGGGGCTTGTCTACGACATGAAAACAAGTGACTATGTTCAATTCCTATGCAACATTGGCTATACTGAAGACCAGATAAACATGATTGTTCTTTGCCCTTCAGGAACTGACACAAGCTGTCCACGAGTGCCCAAATCAAATGCCAATGTAAACTACCCATCAATCACAGTTTCAAATCTCCAGTCCGCTGTGACAATCAAAAGAACCATGCGTAATGTGGGCGAGAACAAGAACGCCATTTATTTTGGGACCATTTCTGAGCCTGATGGAGTGGAGGTAGTGATATGGCCTAGAGTTTTGGTATTCTCCTGGTTCAAGGCGGAAAACACATATTATGTGACGCTCAAACCACAAAAGAAGTCACAAGGAAGATATGATTTTGGAGAGATCGTTTGGTATGATGGCTTCCACAGGGTCAGGAGTCCTTTGGTTGTTTGTGTCAACACTACTCAGGATTCCAATGCTTCCATGAACTTAAGCTACATGTGA

Protein Analysis

787

Amino Acids

85.72

Weight (kDa)

6.83

Isoelectric Point (pI)

40.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 28 - 108 4.9e-17 Peptidase inhibitor I9
Peptidase_S8 PF00082 140 - 594 7.9e-46 Subtilase family
fn3_6 PF17766 674 - 770 9.2e-25 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012494)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26330 AT4G26330
fragaria_vesca FvH4_3g45830
malus_domestica MD03G1002600.v1.1
prunus_persica Prupe.6G002300_v2.0.a1 Prupe.6G002300_v2.0.a1
pyrus_communis pycom03g00250
rosa_chinensis RchiOBHm_Chr5g0083911 RchiOBHm_Chr5g0083921
rosa_laevigata RLG00000037064
rosa_multiflora Rmu_sc0001137.1_g000006
rosa_roxburghii Rroxscaffold_1G00000690
rosa_rugosa Rorug05G0487600 Rorug05G0487600 Rorug05G0487600
rosa_samantha Rh5AG540400 Rh5BG565400 Rh5CG587300
rosa_wichuraiana Rw5G050240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 816
AatII GACGTC 1 cut(s) 429
Acc36I ACCTGC 2 cut(s) 816, 989
AccB1I GGYRCC 1 cut(s) 1841
AccB7I CCANNNNNTGG 1 cut(s) 1212
AccI GTMKAC 1 cut(s) 1887
AccIII TCCGGA 1 cut(s) 1688
AciI CCGC 5 cut(s) 392, 746, 1586, 2061, 2195
AclWI GGATC 5 cut(s) 913, 1010, 1415, 1868, 1881
AcoI YGGCCR 2 cut(s) 270, 418
AcsI RAATTY 3 cut(s) 67, 647, 1648
AcuI CTGAAG 4 cut(s) 120, 1311, 1959, 1962
AcyI GRCGYC 1 cut(s) 426
AfaI GTAC 5 cut(s) 86, 373, 379, 794, 832
AfiI CCNNNNNNNGG 4 cut(s) 513, 1212, 1347, 1864
AflII CTTAAG 1 cut(s) 2350
AflIII ACRYGT 2 cut(s) 1779, 2358
AgsI TTSAA 7 cut(s) 608, 935, 1414, 1515, 1916, 2049, 2191
AhlI ACTAGT 1 cut(s) 374
AjiI CACGTC 3 cut(s) 1203, 1622, 1782
AjnI CCWGG 5 cut(s) 594, 1091, 1346, 1567, 2183
Alw21I GWGCWC 3 cut(s) 586, 772, 1567
Alw26I GTCTC 5 cut(s) 657, 904, 1612, 1690, 1734
AlwI GGATC 5 cut(s) 913, 1010, 1415, 1868, 1881
AlwNI CAGNNNCTG 1 cut(s) 1982
Ama87I CYCGRG 1 cut(s) 759
Aor13HI TCCGGA 1 cut(s) 1688
ApeKI GCWGC 6 cut(s) 366, 389, 851, 1061, 1298, 1736
ApoI RAATTY 3 cut(s) 67, 647, 1648
ArsI GACNNNNNNTTYG 6 cut(s) 1766, 1769, 1798, 1801, 2042, 2074
AspLEI GCGC 1 cut(s) 266
AspS9I GGNCC 9 cut(s) 28, 349, 620, 1010, 1268, 1489, 1520, 1808, 2124
AsuC2I CCSGG 3 cut(s) 760, 761, 1878
AsuHPI GGTGA 2 cut(s) 548, 1295
AsuNHI GCTAGC 1 cut(s) 1306
AvaI CYCGRG 1 cut(s) 759
AvaII GGWCC 3 cut(s) 1268, 1808, 2124
AxyI CCTNAGG 1 cut(s) 774
BaeGI GKGCMC 2 cut(s) 755, 2007
BaeI ACNNNNGTAYC 2 cut(s) 914, 947
BalI TGGCCA 2 cut(s) 272, 420
BamHI GGATCC 1 cut(s) 1873
BanI GGYRCC 1 cut(s) 1841
BanII GRGCYC 1 cut(s) 105
BarI GAAGNNNNNNTAC 2 cut(s) 2264, 2296
BauI CACGAG 1 cut(s) 1998
BbsI GAAGAC 2 cut(s) 618, 1950
Bbv12I GWGCWC 3 cut(s) 586, 772, 1567
BbvCI CCTCAGC 1 cut(s) 126
BbvI GCAGC 6 cut(s) 378, 401, 863, 1048, 1285, 1748
BccI CCATC 8 cut(s) 271, 869, 1027, 1799, 1868, 2041, 2135, 2270
BcgI CGANNNNNNTGC 2 cut(s) 1385, 1419
BciT130I CCWGG 5 cut(s) 596, 1093, 1348, 1569, 2185
BclI TGATCA 1 cut(s) 360
BcnI CCSGG 3 cut(s) 760, 761, 1878
BcoDI GTCTC 5 cut(s) 657, 904, 1612, 1690, 1734
BcuI ACTAGT 1 cut(s) 374
BfaI CTAG 9 cut(s) 6, 375, 660, 785, 1103, 1307, 1494, 1799, 2166
BfmI CTRYAG 2 cut(s) 314, 1734
BfoI RGCGCY 1 cut(s) 267
BfrI CTTAAG 1 cut(s) 2350
BfuAI ACCTGC 2 cut(s) 816, 989
BisI GCNGC 8 cut(s) 367, 390, 393, 852, 1062, 1299, 1587, 1737
BlsI GCNGC 8 cut(s) 368, 391, 394, 853, 1063, 1300, 1588, 1738
BmcAI AGTACT 2 cut(s) 373, 379
Bme1390I CCNGG 8 cut(s) 596, 760, 761, 1093, 1348, 1569, 1878, 2185
Bme18I GGWCC 3 cut(s) 1268, 1808, 2124
BmeT110I CYCGRG 1 cut(s) 759
BmgBI CACGTC 3 cut(s) 1203, 1622, 1782
BmgT120I GGNCC 9 cut(s) 28, 349, 620, 1010, 1268, 1489, 1520, 1808, 2124
BmiI GGNNCC 7 cut(s) 29, 1049, 1491, 1810, 1843, 1875, 2125
BmrFI CCNGG 8 cut(s) 596, 760, 761, 1093, 1348, 1569, 1878, 2185
BmsI GCATC 3 cut(s) 853, 1159, 1325
BmtI GCTAGC 1 cut(s) 1310
BpiI GAAGAC 2 cut(s) 618, 1950
BpmI CTGGAG 3 cut(s) 855, 1551, 2039
Bpu10I CCTNAGC 1 cut(s) 126
BpuEI CTTGAG 3 cut(s) 1218, 1412, 1532
BpuMI CCSGG 3 cut(s) 760, 761, 1878
BsaHI GRCGYC 1 cut(s) 426
BsaI GGTCTC 2 cut(s) 904, 1734
BsaJI CCNNGG 7 cut(s) 267, 759, 760, 1043, 1347, 1568, 1877
BsaWI WCCGGW 2 cut(s) 567, 1688
Bsc4I CCNNNNNNNGG 4 cut(s) 513, 1212, 1347, 1864
Bse118I RCCGGY 1 cut(s) 1844
Bse1I ACTGG 5 cut(s) 174, 838, 1491, 1649, 2056
Bse21I CCTNAGG 1 cut(s) 774
Bse3DI GCAATG 3 cut(s) 401, 972, 1875
BseAI TCCGGA 1 cut(s) 1688
BseBI CCWGG 5 cut(s) 596, 1093, 1348, 1569, 2185
BseDI CCNNGG 7 cut(s) 267, 759, 760, 1043, 1347, 1568, 1877
BseGI GGATG 3 cut(s) 190, 1368, 1578
BseLI CCNNNNNNNGG 4 cut(s) 513, 1212, 1347, 1864
BseMI GCAATG 3 cut(s) 401, 972, 1875
BseMII CTCAG 8 cut(s) 29, 140, 240, 1008, 1272, 1389, 2124, 2339
BseNI ACTGG 5 cut(s) 174, 838, 1491, 1649, 2056
BseSI GKGCMC 2 cut(s) 755, 2007
BseXI GCAGC 6 cut(s) 378, 401, 863, 1048, 1285, 1748
BsgI GTGCAG 3 cut(s) 408, 1469, 1698
BshNI GGYRCC 1 cut(s) 1841
BsiHKAI GWGCWC 3 cut(s) 586, 772, 1567
BsiHKCI CYCGRG 1 cut(s) 759
BsiSI CCGG 5 cut(s) 568, 760, 1689, 1845, 1877
BslFI GGGAC 5 cut(s) 353, 694, 822, 1414, 2137
BslI CCNNNNNNNGG 4 cut(s) 513, 1212, 1347, 1864
BsmAI GTCTC 5 cut(s) 657, 904, 1612, 1690, 1734
BsmBI CGTCTC 1 cut(s) 1612
BsmFI GGGAC 5 cut(s) 353, 694, 822, 1414, 2137
Bso31I GGTCTC 2 cut(s) 904, 1734
BsoBI CYCGRG 1 cut(s) 759
Bsp1286I GDGCHC 6 cut(s) 105, 586, 755, 772, 1567, 2007
Bsp13I TCCGGA 1 cut(s) 1688
Bsp1407I TGTACA 2 cut(s) 84, 792
Bsp143I GATC 7 cut(s) 360, 653, 905, 1015, 1407, 1873, 2262
BspACI CCGC 5 cut(s) 392, 746, 1586, 2061, 2195
BspCNI CTCAG 8 cut(s) 28, 139, 239, 1007, 1271, 1388, 2125, 2338
BspEI TCCGGA 1 cut(s) 1688
BspHI TCATGA 2 cut(s) 118, 1755
BspLI GGNNCC 7 cut(s) 29, 1049, 1491, 1810, 1843, 1875, 2125
BspMAI CTGCAG 2 cut(s) 318, 1738
BspMI ACCTGC 2 cut(s) 816, 989
BspOI GCTAGC 1 cut(s) 1310
BspPI GGATC 5 cut(s) 913, 1010, 1415, 1868, 1881
BspQI GCTCTTC 1 cut(s) 477
BspT107I GGYRCC 1 cut(s) 1841
BspTI CTTAAG 1 cut(s) 2350
BspTNI GGTCTC 2 cut(s) 904, 1734
BsrDI GCAATG 3 cut(s) 401, 972, 1875
BsrFI RCCGGY 1 cut(s) 1844
BsrGI TGTACA 2 cut(s) 84, 792
BsrI ACTGG 5 cut(s) 174, 838, 1491, 1649, 2056
BssAI RCCGGY 1 cut(s) 1844
BssECI CCNNGG 7 cut(s) 267, 759, 760, 1043, 1347, 1568, 1877
BssMI GATC 7 cut(s) 360, 653, 905, 1015, 1407, 1873, 2262
BssNI GRCGYC 1 cut(s) 426
BssSI CACGAG 1 cut(s) 1998
BssT1I CCWWGG 2 cut(s) 267, 1043
Bst2BI CACGAG 1 cut(s) 1998
Bst2UI CCWGG 5 cut(s) 596, 1093, 1348, 1569, 2185
Bst4CI ACNGT 3 cut(s) 195, 1272, 2044
Bst6I CTCTTC 3 cut(s) 174, 477, 1516
BstACI GRCGYC 1 cut(s) 426
BstAFI CTTAAG 1 cut(s) 2350
BstAUI TGTACA 2 cut(s) 84, 792
BstC8I GCNNGC 6 cut(s) 262, 552, 562, 586, 1174, 1308
BstF5I GGATG 3 cut(s) 190, 1368, 1578
BstH2I RGCGCY 1 cut(s) 267
BstHHI GCGC 1 cut(s) 266
BstKTI GATC 7 cut(s) 363, 656, 908, 1018, 1410, 1876, 2265
BstMAI GTCTC 5 cut(s) 657, 904, 1612, 1690, 1734
BstMBI GATC 7 cut(s) 360, 653, 905, 1015, 1407, 1873, 2262
BstNI CCWGG 5 cut(s) 596, 1093, 1348, 1569, 2185
BstNSI RCATGY 2 cut(s) 564, 2362
BstSCI CCNGG 8 cut(s) 594, 758, 759, 1091, 1346, 1567, 1876, 2183
BstSFI CTRYAG 2 cut(s) 314, 1734
BstSLI GKGCMC 2 cut(s) 755, 2007
BstV1I GCAGC 6 cut(s) 378, 401, 863, 1048, 1285, 1748
BstV2I GAAGAC 2 cut(s) 618, 1950
BstX2I RGATCY 3 cut(s) 1015, 1407, 1873
BstYI RGATCY 3 cut(s) 1015, 1407, 1873
Bsu36I CCTNAGG 1 cut(s) 774
BtrI CACGTC 3 cut(s) 1203, 1622, 1782
BtsCI GGATG 3 cut(s) 190, 1368, 1578
BtsI GCAGTG 4 cut(s) 311, 708, 753, 795
BtsIMutI CAGTG 8 cut(s) 23, 181, 311, 708, 753, 795, 917, 1484
BveI ACCTGC 2 cut(s) 816, 989
Cac8I GCNNGC 6 cut(s) 262, 552, 562, 586, 1174, 1308
CaiI CAGNNNCTG 1 cut(s) 1982
CciI TCATGA 2 cut(s) 118, 1755
CfoI GCGC 1 cut(s) 266
Cfr10I RCCGGY 1 cut(s) 1844
Cfr13I GGNCC 9 cut(s) 28, 349, 620, 1010, 1268, 1489, 1520, 1808, 2124
Cfr9I CCCGGG 1 cut(s) 759
CseI GACGC 2 cut(s) 885, 2224
Csp6I GTAC 5 cut(s) 85, 372, 378, 793, 831
CspCI CAANNNNNGTGG 2 cut(s) 684, 719
CviQI GTAC 5 cut(s) 85, 372, 378, 793, 831
DpnI GATC 7 cut(s) 362, 655, 907, 1017, 1409, 1875, 2264
DpnII GATC 7 cut(s) 360, 653, 905, 1015, 1407, 1873, 2262
EaeI YGGCCR 2 cut(s) 270, 418
Eam1104I CTCTTC 3 cut(s) 174, 477, 1516
EarI CTCTTC 3 cut(s) 174, 477, 1516
EciI GGCGGA 1 cut(s) 2210
Eco130I CCWWGG 2 cut(s) 267, 1043
Eco147I AGGCCT 1 cut(s) 499
Eco24I GRGCYC 1 cut(s) 105
Eco31I GGTCTC 2 cut(s) 904, 1734
Eco32I GATATC 1 cut(s) 291
Eco47I GGWCC 3 cut(s) 1268, 1808, 2124
Eco57I CTGAAG 4 cut(s) 120, 1311, 1959, 1962
Eco81I CCTNAGG 1 cut(s) 774
Eco88I CYCGRG 1 cut(s) 759
EcoO109I RGGNCCY 1 cut(s) 28
EcoRII CCWGG 5 cut(s) 594, 1091, 1346, 1567, 2183
EcoRV GATATC 1 cut(s) 291
EcoT14I CCWWGG 2 cut(s) 267, 1043
EcoT22I ATGCAT 1 cut(s) 1318
EcoT38I GRGCYC 1 cut(s) 105
ErhI CCWWGG 2 cut(s) 267, 1043
Esp3I CGTCTC 1 cut(s) 1612
FaqI GGGAC 5 cut(s) 353, 694, 822, 1414, 2137
FauNDI CATATG 2 cut(s) 415, 492
FbaI TGATCA 1 cut(s) 360
FblI GTMKAC 1 cut(s) 1887
Fnu4HI GCNGC 8 cut(s) 367, 390, 393, 852, 1062, 1299, 1587, 1737
FokI GGATG 3 cut(s) 197, 1355, 1585
FriOI GRGCYC 1 cut(s) 105
Fsp4HI GCNGC 8 cut(s) 367, 390, 393, 852, 1062, 1299, 1587, 1737
FspBI CTAG 9 cut(s) 6, 375, 660, 785, 1103, 1307, 1494, 1799, 2166
GlaI GCGC 1 cut(s) 265
GluI GCNGC 8 cut(s) 367, 390, 393, 852, 1062, 1299, 1587, 1737
GsuI CTGGAG 3 cut(s) 855, 1551, 2039
HaeII RGCGCY 1 cut(s) 267
HapII CCGG 5 cut(s) 568, 760, 1689, 1845, 1877
HgaI GACGC 2 cut(s) 885, 2224
HhaI GCGC 1 cut(s) 266
Hin1I GRCGYC 1 cut(s) 426
Hin6I GCGC 1 cut(s) 264
HinP1I GCGC 1 cut(s) 264
HincII GTYRAC 3 cut(s) 1387, 1642, 2317
HindII GTYRAC 3 cut(s) 1387, 1642, 2317
HinfI GANTC 9 cut(s) 11, 464, 935, 1096, 1402, 1427, 1497, 2296, 2330
HpaI GTTAAC 1 cut(s) 1642
HpaII CCGG 5 cut(s) 568, 760, 1689, 1845, 1877
HphI GGTGA 2 cut(s) 548, 1295
Hpy166II GTNNAC 9 cut(s) 85, 793, 831, 1387, 1642, 1888, 1997, 2026, 2317
Hpy188I TCNGA 8 cut(s) 139, 302, 469, 479, 940, 1407, 1825, 2134
Hpy8I GTNNAC 9 cut(s) 85, 793, 831, 1387, 1642, 1888, 1997, 2026, 2317
HpyAV CCTTC 6 cut(s) 362, 511, 593, 935, 1001, 1983
HpyCH4III ACNGT 3 cut(s) 195, 1272, 2044
HpyCH4IV ACGT 4 cut(s) 426, 1202, 1621, 1781
HpySE526I ACGT 4 cut(s) 426, 1202, 1621, 1781
Hsp92I GRCGYC 1 cut(s) 426
HspAI GCGC 1 cut(s) 264
Kpn2I TCCGGA 1 cut(s) 1688
Ksp22I TGATCA 1 cut(s) 360
KspAI GTTAAC 1 cut(s) 1642
Kzo9I GATC 7 cut(s) 360, 653, 905, 1015, 1407, 1873, 2262
LguI GCTCTTC 1 cut(s) 477
LmnI GCTCC 6 cut(s) 159, 767, 836, 1045, 1047, 1570
Lsp1109I GCAGC 6 cut(s) 378, 401, 863, 1048, 1285, 1748
LweI GCATC 3 cut(s) 853, 1159, 1325
MaeI CTAG 9 cut(s) 6, 375, 660, 785, 1103, 1307, 1494, 1799, 2166
MaeII ACGT 4 cut(s) 426, 1202, 1621, 1781
MaeIII GTNAC 9 cut(s) 674, 1001, 1027, 1482, 1847, 1904, 2065, 2212, 2235
MalI GATC 7 cut(s) 362, 655, 907, 1017, 1409, 1875, 2264
MboI GATC 7 cut(s) 360, 653, 905, 1015, 1407, 1873, 2262
MboII GAAGA 8 cut(s) 191, 293, 494, 620, 623, 1503, 1955, 2256
MfeI CAATTG 2 cut(s) 1071, 1210
MflI RGATCY 3 cut(s) 1015, 1407, 1873
MhlI GDGCHC 6 cut(s) 105, 586, 755, 772, 1567, 2007
MlsI TGGCCA 2 cut(s) 272, 420
MluNI TGGCCA 2 cut(s) 272, 420
MlyI GAGTC 3 cut(s) 1396, 1506, 2305
MmeI TCCRAC 2 cut(s) 1634, 1848
Mox20I TGGCCA 2 cut(s) 272, 420
Mph1103I ATGCAT 1 cut(s) 1318
MroI TCCGGA 1 cut(s) 1688
MscI TGGCCA 2 cut(s) 272, 420
MseI TTAA 7 cut(s) 189, 1136, 1152, 1185, 1641, 1743, 2351
Msp20I TGGCCA 2 cut(s) 272, 420
MspA1I CMGCKG 3 cut(s) 130, 392, 2063
MspCI CTTAAG 1 cut(s) 2350
MspI CCGG 5 cut(s) 568, 760, 1689, 1845, 1877
MspR9I CCNGG 8 cut(s) 596, 760, 761, 1093, 1348, 1569, 1878, 2185
MunI CAATTG 2 cut(s) 1071, 1210
MvaI CCWGG 5 cut(s) 596, 1093, 1348, 1569, 2185
NciI CCSGG 3 cut(s) 760, 761, 1878
NdeI CATATG 2 cut(s) 415, 492
NdeII GATC 7 cut(s) 360, 653, 905, 1015, 1407, 1873, 2262
NheI GCTAGC 1 cut(s) 1306
NlaIV GGNNCC 7 cut(s) 29, 1049, 1491, 1810, 1843, 1875, 2125
NmuCI GTSAC 8 cut(s) 674, 1027, 1482, 1847, 1904, 2065, 2212, 2235
NsiI ATGCAT 1 cut(s) 1318
NspI RCATGY 2 cut(s) 564, 2362
PaeI GCATGC 1 cut(s) 564
PagI TCATGA 2 cut(s) 118, 1755
PaqCI CACCTGC 1 cut(s) 816
PceI AGGCCT 1 cut(s) 499
PciI ACATGT 1 cut(s) 2358
PciSI GCTCTTC 1 cut(s) 477
PfeI GAWTC 6 cut(s) 11, 464, 935, 1096, 1427, 2330
PflMI CCANNNNNTGG 1 cut(s) 1212
PkrI GCNGC 8 cut(s) 368, 391, 394, 853, 1063, 1300, 1588, 1738
PleI GAGTC 3 cut(s) 1396, 1505, 2304
PpsI GAGTC 3 cut(s) 1396, 1505, 2304
PscI ACATGT 1 cut(s) 2358
Psp6I CCWGG 5 cut(s) 594, 1091, 1346, 1567, 2183
PspGI CCWGG 5 cut(s) 594, 1091, 1346, 1567, 2183
PspN4I GGNNCC 7 cut(s) 29, 1049, 1491, 1810, 1843, 1875, 2125
PspPI GGNCC 9 cut(s) 28, 349, 620, 1010, 1268, 1489, 1520, 1808, 2124
PsrI GAACNNNNNNTAC 1 cut(s) 2339
PstI CTGCAG 2 cut(s) 318, 1738
PstNI CAGNNNCTG 1 cut(s) 1982
PsuI RGATCY 3 cut(s) 1015, 1407, 1873
PvuII CAGCTG 1 cut(s) 130
RsaI GTAC 5 cut(s) 86, 373, 379, 794, 832
RsaNI GTAC 5 cut(s) 85, 372, 378, 793, 831
SapI GCTCTTC 1 cut(s) 477
SaqAI TTAA 7 cut(s) 189, 1136, 1152, 1185, 1641, 1743, 2351
SatI GCNGC 8 cut(s) 367, 390, 393, 852, 1062, 1299, 1587, 1737
Sau3AI GATC 7 cut(s) 360, 653, 905, 1015, 1407, 1873, 2262
Sau96I GGNCC 9 cut(s) 28, 349, 620, 1010, 1268, 1489, 1520, 1808, 2124
ScaI AGTACT 2 cut(s) 373, 379
SchI GAGTC 3 cut(s) 1396, 1506, 2305
ScrFI CCNGG 8 cut(s) 596, 760, 761, 1093, 1348, 1569, 1878, 2185
SduI GDGCHC 6 cut(s) 105, 586, 755, 772, 1567, 2007
SfaNI GCATC 3 cut(s) 853, 1159, 1325
SfcI CTRYAG 2 cut(s) 314, 1734
SinI GGWCC 3 cut(s) 1268, 1808, 2124
SmaI CCCGGG 1 cut(s) 761
SmlI CTYRAG 4 cut(s) 1233, 1391, 1547, 2350
SmoI CTYRAG 4 cut(s) 1233, 1391, 1547, 2350
SpeI ACTAGT 1 cut(s) 374
SphI GCATGC 1 cut(s) 564
SseBI AGGCCT 1 cut(s) 499
SsiI CCGC 5 cut(s) 392, 746, 1586, 2061, 2195
SspI AATATT 1 cut(s) 115
SspMI CTAG 9 cut(s) 6, 375, 660, 785, 1103, 1307, 1494, 1799, 2166
StuI AGGCCT 1 cut(s) 499
StyD4I CCNGG 8 cut(s) 594, 758, 759, 1091, 1346, 1567, 1876, 2183
StyI CCWWGG 2 cut(s) 267, 1043
TaaI ACNGT 3 cut(s) 195, 1272, 2044
TaiI ACGT 4 cut(s) 429, 1205, 1624, 1784
TaqI TCGA 3 cut(s) 71, 652, 908
TatI WGTACW 5 cut(s) 84, 371, 377, 792, 830
TauI GCSGC 2 cut(s) 395, 1589
TfiI GAWTC 6 cut(s) 11, 464, 935, 1096, 1427, 2330
Tru1I TTAA 7 cut(s) 189, 1136, 1152, 1185, 1641, 1743, 2351
Tru9I TTAA 7 cut(s) 189, 1136, 1152, 1185, 1641, 1743, 2351
TscAI CASTG 8 cut(s) 23, 181, 318, 708, 760, 795, 924, 1491
TseFI GTSAC 8 cut(s) 674, 1027, 1482, 1847, 1904, 2065, 2212, 2235
TseI GCWGC 6 cut(s) 366, 389, 851, 1061, 1298, 1736
Tsp45I GTSAC 8 cut(s) 674, 1027, 1482, 1847, 1904, 2065, 2212, 2235
TspMI CCCGGG 1 cut(s) 759
TspRI CASTG 8 cut(s) 23, 181, 318, 708, 760, 795, 924, 1491
Van91I CCANNNNNTGG 1 cut(s) 1212
Vha464I CTTAAG 1 cut(s) 2350
VpaK11BI GGWCC 3 cut(s) 1268, 1808, 2124
XapI RAATTY 3 cut(s) 67, 647, 1648
XceI RCATGY 2 cut(s) 564, 2362
XcmI CCANNNNNNNNNTGG 2 cut(s) 1867, 2089
XmaI CCCGGG 1 cut(s) 759
XmiI GTMKAC 1 cut(s) 1887
XspI CTAG 9 cut(s) 6, 375, 660, 785, 1103, 1307, 1494, 1799, 2166
ZraI GACGTC 1 cut(s) 427
ZrmI AGTACT 2 cut(s) 373, 379
Zsp2I ATGCAT 1 cut(s) 1318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.