Rw5G050240

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0012494
Physical Location & Seq
Forward (+)
86819899 .. 86823483
3585 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G050240.1

Sequence Viewer

Length: 2166 bp
ATGCTTTATAGCTACAAGCATAGTTTCTCAGGGTTTTCGGCAAAGCTCAATTCATCACAAGCAAGCGCCTTGGCCAAGATGGAAGAAGTGATATCGGTTTTCAGAAGTAAGACACTGCAGTTGCACACAACAAGGAGTTGGGACTTCTTGGGCCTTCCCTTGATCAGCAGCAGTACTAGAACTGCAAGTGCAGCGGCCTCTCCATTGCAATTAGCATATGGCCAAGACGTCATTGTTGGGGTTTTTGATACAGGTATATGGCCTGAATCAGAAAGTTTCCGAGAAGAGCCACATATGAGGCCTATCCCCTCAACTTGGAAGGGAAAGTGTGTGAAAGGTGAGAAGTTTGAGCCAGCAAAAGCATGCAACCGGAAGCTAATTGGTGCTCGCTACTACCTGGAAGGCTTTGAAGAAGACTATGGGCCACTAAACAAGAGTGGCAATCCAGAATTTCGATCAGCTAGAGACTTTCTTGGTCACGGGACACACACAGCCTCAACAGCAGTGGGTTCAGTAGTGAAAAACAATGCAAGTTTCTATGGTTTTGCAGAGGGCACTGCCCGGGGTGGAGCACCTAAGGCTCGTCTAGCAGTGTACAAAGTTTGTTGGGGACAGAATTATAGTAGCAGGTGTACTGGAGCAGACATACTTGCAGCTTTTGATGATGCTTTGCATGATGGAGTTCATGTTATTTCAGCGTCTATTGGATCGAGACCACCACTGATACCATTGTTTGAATCAGAAGGTGCTATCGGTTCGTTTCATGCAATGCAGTTAGGTGTTAGTGTGGTGTTCTCAGCAGGTAACGAAGGGCCAGATCCATCACAGGTCACAAATGTTGCTCCTTGGAGCCTCTGTGTTGCTGCTTCATCAATTGATAGAATGTTCCCAACCAGGATTCTCCTAGATAATAAGCTCTCTATCATGGGAGAGAGCTTAATTACTACACCTATTAAAGCAAAATTGGCAGATGCGAGCGTATTCTTTAATAATGGGGTTTGCACGTCCAGCAATTGGAAAAAACCAAAATCTGCTCAAGGTAGAGTAATCCTATGCTTCTCAGCCATAGGACCGTATGATATTGGTGAGGCTGAAGTGGCTGCCAAGCTAGCCAATGCATCAGCTTTGATATTTGTAGAACCAATGTCCAGGGAGATTGGTGCTGACATCATCCCCTCAGTCCATGTCAACCTTGAGCAAGGGACTCGGATCTTGAACTATCTTACTGAATCTCCCACGAAGCCTGTTGTGCAGATAGAAGCAAGTAAAACTATTATCGGGAAGTCACTGGCCCCTAGAGTCGCATACTTCTCTTCAAGAGGCCCCAGCTCACTTACACCTGATATTCTCAAGCCAGATATAAGTGCTCCAGGGATGAACATATTAGCGGCATGGCCTAATCAAACTTCTCCAACTTTGAGACGTGATGATAACCGTTCAGTTAACTGGAATTTTCAGTCAGGAACATCAATGTCCTGTCCTCATGTCTCCGGAGTTGTTGCTCTCATCAAATCTGCACACCCAAATTGGTCTCCTGCAGCCATTAAATCTGCTATCATGACCACAGCATACACAAAGGACACGTCATTTGACACCATTCTAGCCGATGGACCCATGAAAGCCTCCGACCCTTTTGACTTAGGTGCCGGTCACATAGACCCCATCAAGGCAATGGATCCGGGGCTTGTCTACGACATGAAAACAAGTGACTATGTTCAATTCCTATGCAACATTGGCTATACTGAAGACCAGATAAACATGATTGTTCTTTGCCCTTCAGGAACTGACACAAGCTGTCCACGAGTGCCCAAATCAAATGCCAATGTAAACTACCCATCAATCACAGTTTCAAATCTCCAGTCCGCTGTGACAATCAAAAGAACCATGCGTAATGTGGGCGAGAACAAGAACGCCATTTATTTTGGGACCATTTCTGAGCCTGATGGAGTGGAGGTAGTGATATGGCCTAGAGTTTTGGTATTCTCCTGGTTCAAGGCGGAAAACACATATTATGTGACGCTCAAACCACAAAAGAAGTCACAAGGAAGATATGATTTTGGAGAGATCGTTTGGTATGATGGCTTCCACAGGGTCAGGAGTCCTTTGGTTGTTTGTGTCAACACTACTCAGGATTCCAATGCTTCCATGAACTTAAGCTACATGTGA

Protein Analysis

721

Amino Acids

78.26

Weight (kDa)

8.29

Isoelectric Point (pI)

38.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 1 - 42 1.7e-11 Peptidase inhibitor I9
Peptidase_S8 PF00082 74 - 528 6e-46 Subtilase family
fn3_6 PF17766 608 - 704 8.1e-25 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012494)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26330 AT4G26330
fragaria_vesca FvH4_3g45830
malus_domestica MD03G1002600.v1.1
prunus_persica Prupe.6G002300_v2.0.a1 Prupe.6G002300_v2.0.a1
pyrus_communis pycom03g00250
rosa_chinensis RchiOBHm_Chr5g0083911 RchiOBHm_Chr5g0083921
rosa_laevigata RLG00000037064
rosa_multiflora Rmu_sc0001137.1_g000006
rosa_roxburghii Rroxscaffold_1G00000690
rosa_rugosa Rorug05G0487600 Rorug05G0487600 Rorug05G0487600
rosa_samantha Rh5AG540400 Rh5BG565400 Rh5CG587300
rosa_wichuraiana Rw5G050240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 618
AatII GACGTC 1 cut(s) 231
Acc36I ACCTGC 2 cut(s) 618, 791
AccB1I GGYRCC 1 cut(s) 1643
AccB7I CCANNNNNTGG 1 cut(s) 1014
AccI GTMKAC 1 cut(s) 1689
AccIII TCCGGA 1 cut(s) 1490
AciI CCGC 4 cut(s) 194, 1388, 1863, 1997
AclWI GGATC 5 cut(s) 715, 812, 1217, 1670, 1683
AcoI YGGCCR 2 cut(s) 72, 220
AcsI RAATTY 2 cut(s) 449, 1450
AcuI CTGAAG 3 cut(s) 1113, 1761, 1764
AcyI GRCGYC 1 cut(s) 228
AfaI GTAC 3 cut(s) 175, 596, 634
AfiI CCNNNNNNNGG 3 cut(s) 315, 1014, 1666
AflII CTTAAG 1 cut(s) 2152
AflIII ACRYGT 2 cut(s) 1581, 2160
AgsI TTSAA 7 cut(s) 410, 737, 1216, 1317, 1718, 1851, 1993
AjiI CACGTC 3 cut(s) 1005, 1424, 1584
AjnI CCWGG 5 cut(s) 396, 893, 1148, 1369, 1985
Alw21I GWGCWC 3 cut(s) 388, 574, 1369
Alw26I GTCTC 5 cut(s) 459, 706, 1414, 1492, 1536
AlwI GGATC 5 cut(s) 715, 812, 1217, 1670, 1683
AlwNI CAGNNNCTG 1 cut(s) 1784
Ama87I CYCGRG 1 cut(s) 561
Aor13HI TCCGGA 1 cut(s) 1490
ApeKI GCWGC 6 cut(s) 168, 191, 653, 863, 1100, 1538
ApoI RAATTY 2 cut(s) 449, 1450
ArsI GACNNNNNNTTYG 6 cut(s) 1568, 1571, 1600, 1603, 1844, 1876
AspLEI GCGC 1 cut(s) 68
AspS9I GGNCC 8 cut(s) 151, 422, 812, 1070, 1291, 1322, 1610, 1926
AsuC2I CCSGG 3 cut(s) 562, 563, 1680
AsuHPI GGTGA 2 cut(s) 350, 1097
AsuNHI GCTAGC 1 cut(s) 1108
AvaI CYCGRG 1 cut(s) 561
AvaII GGWCC 3 cut(s) 1070, 1610, 1926
AxyI CCTNAGG 1 cut(s) 576
BaeGI GKGCMC 2 cut(s) 557, 1809
BaeI ACNNNNGTAYC 2 cut(s) 716, 749
BalI TGGCCA 2 cut(s) 74, 222
BamHI GGATCC 1 cut(s) 1675
BanI GGYRCC 1 cut(s) 1643
BarI GAAGNNNNNNTAC 2 cut(s) 2066, 2098
BauI CACGAG 1 cut(s) 1800
BbsI GAAGAC 2 cut(s) 420, 1752
Bbv12I GWGCWC 3 cut(s) 388, 574, 1369
BbvI GCAGC 6 cut(s) 180, 203, 665, 850, 1087, 1550
BccI CCATC 8 cut(s) 73, 671, 829, 1601, 1670, 1843, 1937, 2072
BcgI CGANNNNNNTGC 2 cut(s) 1187, 1221
BciT130I CCWGG 5 cut(s) 398, 895, 1150, 1371, 1987
BclI TGATCA 1 cut(s) 162
BcnI CCSGG 3 cut(s) 562, 563, 1680
BcoDI GTCTC 5 cut(s) 459, 706, 1414, 1492, 1536
BfaI CTAG 8 cut(s) 177, 462, 587, 905, 1109, 1296, 1601, 1968
BfmI CTRYAG 2 cut(s) 116, 1536
BfoI RGCGCY 1 cut(s) 69
BfrI CTTAAG 1 cut(s) 2152
BfuAI ACCTGC 2 cut(s) 618, 791
BisI GCNGC 8 cut(s) 169, 192, 195, 654, 864, 1101, 1389, 1539
BlsI GCNGC 8 cut(s) 170, 193, 196, 655, 865, 1102, 1390, 1540
BmcAI AGTACT 1 cut(s) 175
Bme1390I CCNGG 8 cut(s) 398, 562, 563, 895, 1150, 1371, 1680, 1987
Bme18I GGWCC 3 cut(s) 1070, 1610, 1926
BmeT110I CYCGRG 1 cut(s) 561
BmgBI CACGTC 3 cut(s) 1005, 1424, 1584
BmgT120I GGNCC 8 cut(s) 151, 422, 812, 1070, 1291, 1322, 1610, 1926
BmiI GGNNCC 7 cut(s) 851, 1293, 1324, 1612, 1645, 1677, 1927
BmrFI CCNGG 8 cut(s) 398, 562, 563, 895, 1150, 1371, 1680, 1987
BmsI GCATC 3 cut(s) 655, 961, 1127
BmtI GCTAGC 1 cut(s) 1112
BpiI GAAGAC 2 cut(s) 420, 1752
BpmI CTGGAG 3 cut(s) 657, 1353, 1841
BpuEI CTTGAG 3 cut(s) 1020, 1214, 1334
BpuMI CCSGG 3 cut(s) 562, 563, 1680
BsaHI GRCGYC 1 cut(s) 228
BsaI GGTCTC 2 cut(s) 706, 1536
BsaJI CCNNGG 7 cut(s) 69, 561, 562, 845, 1149, 1370, 1679
BsaWI WCCGGW 2 cut(s) 369, 1490
Bsc4I CCNNNNNNNGG 3 cut(s) 315, 1014, 1666
Bse118I RCCGGY 1 cut(s) 1646
Bse1I ACTGG 4 cut(s) 640, 1293, 1451, 1858
Bse21I CCTNAGG 1 cut(s) 576
Bse3DI GCAATG 3 cut(s) 203, 774, 1677
BseAI TCCGGA 1 cut(s) 1490
BseBI CCWGG 5 cut(s) 398, 895, 1150, 1371, 1987
BseDI CCNNGG 7 cut(s) 69, 561, 562, 845, 1149, 1370, 1679
BseGI GGATG 2 cut(s) 1170, 1380
BseLI CCNNNNNNNGG 3 cut(s) 315, 1014, 1666
BseMI GCAATG 3 cut(s) 203, 774, 1677
BseMII CTCAG 6 cut(s) 42, 810, 1074, 1191, 1926, 2141
BseNI ACTGG 4 cut(s) 640, 1293, 1451, 1858
BseSI GKGCMC 2 cut(s) 557, 1809
BseXI GCAGC 6 cut(s) 180, 203, 665, 850, 1087, 1550
BseYI CCCAGC 1 cut(s) 1325
BsgI GTGCAG 3 cut(s) 210, 1271, 1500
BshNI GGYRCC 1 cut(s) 1643
BsiHKAI GWGCWC 3 cut(s) 388, 574, 1369
BsiHKCI CYCGRG 1 cut(s) 561
BsiSI CCGG 5 cut(s) 370, 562, 1491, 1647, 1679
BslFI GGGAC 5 cut(s) 155, 496, 624, 1216, 1939
BslI CCNNNNNNNGG 3 cut(s) 315, 1014, 1666
BsmAI GTCTC 5 cut(s) 459, 706, 1414, 1492, 1536
BsmBI CGTCTC 1 cut(s) 1414
BsmFI GGGAC 5 cut(s) 155, 496, 624, 1216, 1939
Bso31I GGTCTC 2 cut(s) 706, 1536
BsoBI CYCGRG 1 cut(s) 561
Bsp1286I GDGCHC 5 cut(s) 388, 557, 574, 1369, 1809
Bsp13I TCCGGA 1 cut(s) 1490
Bsp1407I TGTACA 1 cut(s) 594
Bsp143I GATC 7 cut(s) 162, 455, 707, 817, 1209, 1675, 2064
BspACI CCGC 4 cut(s) 194, 1388, 1863, 1997
BspCNI CTCAG 6 cut(s) 41, 809, 1073, 1190, 1927, 2140
BspEI TCCGGA 1 cut(s) 1490
BspHI TCATGA 1 cut(s) 1557
BspLI GGNNCC 7 cut(s) 851, 1293, 1324, 1612, 1645, 1677, 1927
BspMAI CTGCAG 2 cut(s) 120, 1540
BspMI ACCTGC 2 cut(s) 618, 791
BspOI GCTAGC 1 cut(s) 1112
BspPI GGATC 5 cut(s) 715, 812, 1217, 1670, 1683
BspQI GCTCTTC 1 cut(s) 279
BspT107I GGYRCC 1 cut(s) 1643
BspTI CTTAAG 1 cut(s) 2152
BspTNI GGTCTC 2 cut(s) 706, 1536
BsrDI GCAATG 3 cut(s) 203, 774, 1677
BsrFI RCCGGY 1 cut(s) 1646
BsrGI TGTACA 1 cut(s) 594
BsrI ACTGG 4 cut(s) 640, 1293, 1451, 1858
BssAI RCCGGY 1 cut(s) 1646
BssECI CCNNGG 7 cut(s) 69, 561, 562, 845, 1149, 1370, 1679
BssMI GATC 7 cut(s) 162, 455, 707, 817, 1209, 1675, 2064
BssNI GRCGYC 1 cut(s) 228
BssSI CACGAG 1 cut(s) 1800
BssT1I CCWWGG 2 cut(s) 69, 845
Bst2BI CACGAG 1 cut(s) 1800
Bst2UI CCWGG 5 cut(s) 398, 895, 1150, 1371, 1987
Bst4CI ACNGT 3 cut(s) 1074, 1436, 1846
Bst6I CTCTTC 2 cut(s) 279, 1318
BstACI GRCGYC 1 cut(s) 228
BstAFI CTTAAG 1 cut(s) 2152
BstAUI TGTACA 1 cut(s) 594
BstC8I GCNNGC 6 cut(s) 64, 354, 364, 388, 976, 1110
BstDEI CTNAG 8 cut(s) 28, 576, 796, 1060, 1177, 1639, 1935, 2127
BstF5I GGATG 2 cut(s) 1170, 1380
BstH2I RGCGCY 1 cut(s) 69
BstHHI GCGC 1 cut(s) 68
BstKTI GATC 7 cut(s) 165, 458, 710, 820, 1212, 1678, 2067
BstMAI GTCTC 5 cut(s) 459, 706, 1414, 1492, 1536
BstMBI GATC 7 cut(s) 162, 455, 707, 817, 1209, 1675, 2064
BstNI CCWGG 5 cut(s) 398, 895, 1150, 1371, 1987
BstNSI RCATGY 2 cut(s) 366, 2164
BstSCI CCNGG 8 cut(s) 396, 560, 561, 893, 1148, 1369, 1678, 1985
BstSFI CTRYAG 2 cut(s) 116, 1536
BstSLI GKGCMC 2 cut(s) 557, 1809
BstV1I GCAGC 6 cut(s) 180, 203, 665, 850, 1087, 1550
BstV2I GAAGAC 2 cut(s) 420, 1752
BstX2I RGATCY 3 cut(s) 817, 1209, 1675
BstYI RGATCY 3 cut(s) 817, 1209, 1675
Bsu36I CCTNAGG 1 cut(s) 576
BtrI CACGTC 3 cut(s) 1005, 1424, 1584
BtsCI GGATG 2 cut(s) 1170, 1380
BtsI GCAGTG 4 cut(s) 113, 510, 555, 597
BtsIMutI CAGTG 6 cut(s) 113, 510, 555, 597, 719, 1286
BveI ACCTGC 2 cut(s) 618, 791
Cac8I GCNNGC 6 cut(s) 64, 354, 364, 388, 976, 1110
CaiI CAGNNNCTG 1 cut(s) 1784
CciI TCATGA 1 cut(s) 1557
CfoI GCGC 1 cut(s) 68
Cfr10I RCCGGY 1 cut(s) 1646
Cfr13I GGNCC 8 cut(s) 151, 422, 812, 1070, 1291, 1322, 1610, 1926
Cfr9I CCCGGG 1 cut(s) 561
CseI GACGC 2 cut(s) 687, 2026
Csp6I GTAC 3 cut(s) 174, 595, 633
CspCI CAANNNNNGTGG 2 cut(s) 486, 521
CviQI GTAC 3 cut(s) 174, 595, 633
DdeI CTNAG 8 cut(s) 28, 576, 796, 1060, 1177, 1639, 1935, 2127
DpnI GATC 7 cut(s) 164, 457, 709, 819, 1211, 1677, 2066
DpnII GATC 7 cut(s) 162, 455, 707, 817, 1209, 1675, 2064
EaeI YGGCCR 2 cut(s) 72, 220
Eam1104I CTCTTC 2 cut(s) 279, 1318
EarI CTCTTC 2 cut(s) 279, 1318
EciI GGCGGA 1 cut(s) 2012
Eco130I CCWWGG 2 cut(s) 69, 845
Eco147I AGGCCT 1 cut(s) 301
Eco31I GGTCTC 2 cut(s) 706, 1536
Eco32I GATATC 1 cut(s) 93
Eco47I GGWCC 3 cut(s) 1070, 1610, 1926
Eco57I CTGAAG 3 cut(s) 1113, 1761, 1764
Eco81I CCTNAGG 1 cut(s) 576
Eco88I CYCGRG 1 cut(s) 561
EcoO109I RGGNCCY 1 cut(s) 1322
EcoRII CCWGG 5 cut(s) 396, 893, 1148, 1369, 1985
EcoRV GATATC 1 cut(s) 93
EcoT14I CCWWGG 2 cut(s) 69, 845
EcoT22I ATGCAT 1 cut(s) 1120
ErhI CCWWGG 2 cut(s) 69, 845
Esp3I CGTCTC 1 cut(s) 1414
FaqI GGGAC 5 cut(s) 155, 496, 624, 1216, 1939
FauNDI CATATG 2 cut(s) 217, 294
FbaI TGATCA 1 cut(s) 162
FblI GTMKAC 1 cut(s) 1689
Fnu4HI GCNGC 8 cut(s) 169, 192, 195, 654, 864, 1101, 1389, 1539
FokI GGATG 2 cut(s) 1157, 1387
Fsp4HI GCNGC 8 cut(s) 169, 192, 195, 654, 864, 1101, 1389, 1539
FspBI CTAG 8 cut(s) 177, 462, 587, 905, 1109, 1296, 1601, 1968
GlaI GCGC 1 cut(s) 67
GluI GCNGC 8 cut(s) 169, 192, 195, 654, 864, 1101, 1389, 1539
GsaI CCCAGC 1 cut(s) 1329
GsuI CTGGAG 3 cut(s) 657, 1353, 1841
HaeII RGCGCY 1 cut(s) 69
HapII CCGG 5 cut(s) 370, 562, 1491, 1647, 1679
HgaI GACGC 2 cut(s) 687, 2026
HhaI GCGC 1 cut(s) 68
Hin1I GRCGYC 1 cut(s) 228
Hin6I GCGC 1 cut(s) 66
HinP1I GCGC 1 cut(s) 66
HincII GTYRAC 3 cut(s) 1189, 1444, 2119
HindII GTYRAC 3 cut(s) 1189, 1444, 2119
HinfI GANTC 8 cut(s) 266, 737, 898, 1204, 1229, 1299, 2098, 2132
HpaI GTTAAC 1 cut(s) 1444
HpaII CCGG 5 cut(s) 370, 562, 1491, 1647, 1679
HphI GGTGA 2 cut(s) 350, 1097
Hpy166II GTNNAC 8 cut(s) 595, 633, 1189, 1444, 1690, 1799, 1828, 2119
Hpy188I TCNGA 7 cut(s) 104, 271, 281, 742, 1209, 1627, 1936
Hpy8I GTNNAC 8 cut(s) 595, 633, 1189, 1444, 1690, 1799, 1828, 2119
HpyAV CCTTC 6 cut(s) 164, 313, 395, 737, 803, 1785
HpyCH4III ACNGT 3 cut(s) 1074, 1436, 1846
HpyCH4IV ACGT 4 cut(s) 228, 1004, 1423, 1583
HpyF3I CTNAG 8 cut(s) 28, 576, 796, 1060, 1177, 1639, 1935, 2127
HpySE526I ACGT 4 cut(s) 228, 1004, 1423, 1583
Hsp92I GRCGYC 1 cut(s) 228
HspAI GCGC 1 cut(s) 66
Kpn2I TCCGGA 1 cut(s) 1490
Ksp22I TGATCA 1 cut(s) 162
KspAI GTTAAC 1 cut(s) 1444
Kzo9I GATC 7 cut(s) 162, 455, 707, 817, 1209, 1675, 2064
LguI GCTCTTC 1 cut(s) 279
LmnI GCTCC 5 cut(s) 569, 638, 847, 849, 1372
Lsp1109I GCAGC 6 cut(s) 180, 203, 665, 850, 1087, 1550
LweI GCATC 3 cut(s) 655, 961, 1127
MaeI CTAG 8 cut(s) 177, 462, 587, 905, 1109, 1296, 1601, 1968
MaeII ACGT 4 cut(s) 228, 1004, 1423, 1583
MaeIII GTNAC 9 cut(s) 476, 803, 829, 1284, 1649, 1706, 1867, 2014, 2037
MalI GATC 7 cut(s) 164, 457, 709, 819, 1211, 1677, 2066
MboI GATC 7 cut(s) 162, 455, 707, 817, 1209, 1675, 2064
MboII GAAGA 7 cut(s) 95, 296, 422, 425, 1305, 1757, 2058
MfeI CAATTG 2 cut(s) 873, 1012
MflI RGATCY 3 cut(s) 817, 1209, 1675
MhlI GDGCHC 5 cut(s) 388, 557, 574, 1369, 1809
MlsI TGGCCA 2 cut(s) 74, 222
MluNI TGGCCA 2 cut(s) 74, 222
MlyI GAGTC 3 cut(s) 1198, 1308, 2107
MmeI TCCRAC 2 cut(s) 1436, 1650
Mox20I TGGCCA 2 cut(s) 74, 222
Mph1103I ATGCAT 1 cut(s) 1120
MroI TCCGGA 1 cut(s) 1490
MscI TGGCCA 2 cut(s) 74, 222
MseI TTAA 6 cut(s) 938, 954, 987, 1443, 1545, 2153
Msp20I TGGCCA 2 cut(s) 74, 222
MspA1I CMGCKG 2 cut(s) 194, 1865
MspCI CTTAAG 1 cut(s) 2152
MspI CCGG 5 cut(s) 370, 562, 1491, 1647, 1679
MspR9I CCNGG 8 cut(s) 398, 562, 563, 895, 1150, 1371, 1680, 1987
MunI CAATTG 2 cut(s) 873, 1012
MvaI CCWGG 5 cut(s) 398, 895, 1150, 1371, 1987
NciI CCSGG 3 cut(s) 562, 563, 1680
NdeI CATATG 2 cut(s) 217, 294
NdeII GATC 7 cut(s) 162, 455, 707, 817, 1209, 1675, 2064
NheI GCTAGC 1 cut(s) 1108
NlaIV GGNNCC 7 cut(s) 851, 1293, 1324, 1612, 1645, 1677, 1927
NmuCI GTSAC 8 cut(s) 476, 829, 1284, 1649, 1706, 1867, 2014, 2037
NsiI ATGCAT 1 cut(s) 1120
NspI RCATGY 2 cut(s) 366, 2164
PaeI GCATGC 1 cut(s) 366
PagI TCATGA 1 cut(s) 1557
PaqCI CACCTGC 1 cut(s) 618
PceI AGGCCT 1 cut(s) 301
PciI ACATGT 1 cut(s) 2160
PciSI GCTCTTC 1 cut(s) 279
PfeI GAWTC 5 cut(s) 266, 737, 898, 1229, 2132
PflMI CCANNNNNTGG 1 cut(s) 1014
PkrI GCNGC 8 cut(s) 170, 193, 196, 655, 865, 1102, 1390, 1540
PleI GAGTC 3 cut(s) 1198, 1307, 2106
PpsI GAGTC 3 cut(s) 1198, 1307, 2106
PscI ACATGT 1 cut(s) 2160
Psp6I CCWGG 5 cut(s) 396, 893, 1148, 1369, 1985
PspFI CCCAGC 1 cut(s) 1325
PspGI CCWGG 5 cut(s) 396, 893, 1148, 1369, 1985
PspN4I GGNNCC 7 cut(s) 851, 1293, 1324, 1612, 1645, 1677, 1927
PspPI GGNCC 8 cut(s) 151, 422, 812, 1070, 1291, 1322, 1610, 1926
PsrI GAACNNNNNNTAC 1 cut(s) 2141
PstI CTGCAG 2 cut(s) 120, 1540
PstNI CAGNNNCTG 1 cut(s) 1784
PsuI RGATCY 3 cut(s) 817, 1209, 1675
RsaI GTAC 3 cut(s) 175, 596, 634
RsaNI GTAC 3 cut(s) 174, 595, 633
SapI GCTCTTC 1 cut(s) 279
SaqAI TTAA 6 cut(s) 938, 954, 987, 1443, 1545, 2153
SatI GCNGC 8 cut(s) 169, 192, 195, 654, 864, 1101, 1389, 1539
Sau3AI GATC 7 cut(s) 162, 455, 707, 817, 1209, 1675, 2064
Sau96I GGNCC 8 cut(s) 151, 422, 812, 1070, 1291, 1322, 1610, 1926
ScaI AGTACT 1 cut(s) 175
SchI GAGTC 3 cut(s) 1198, 1308, 2107
ScrFI CCNGG 8 cut(s) 398, 562, 563, 895, 1150, 1371, 1680, 1987
SduI GDGCHC 5 cut(s) 388, 557, 574, 1369, 1809
SfaNI GCATC 3 cut(s) 655, 961, 1127
SfcI CTRYAG 2 cut(s) 116, 1536
SinI GGWCC 3 cut(s) 1070, 1610, 1926
SmaI CCCGGG 1 cut(s) 563
SmlI CTYRAG 4 cut(s) 1035, 1193, 1349, 2152
SmoI CTYRAG 4 cut(s) 1035, 1193, 1349, 2152
SphI GCATGC 1 cut(s) 366
SseBI AGGCCT 1 cut(s) 301
SsiI CCGC 4 cut(s) 194, 1388, 1863, 1997
SspMI CTAG 8 cut(s) 177, 462, 587, 905, 1109, 1296, 1601, 1968
StuI AGGCCT 1 cut(s) 301
StyD4I CCNGG 8 cut(s) 396, 560, 561, 893, 1148, 1369, 1678, 1985
StyI CCWWGG 2 cut(s) 69, 845
TaaI ACNGT 3 cut(s) 1074, 1436, 1846
TaiI ACGT 4 cut(s) 231, 1007, 1426, 1586
TaqI TCGA 2 cut(s) 454, 710
TatI WGTACW 3 cut(s) 173, 594, 632
TauI GCSGC 2 cut(s) 197, 1391
TfiI GAWTC 5 cut(s) 266, 737, 898, 1229, 2132
Tru1I TTAA 6 cut(s) 938, 954, 987, 1443, 1545, 2153
Tru9I TTAA 6 cut(s) 938, 954, 987, 1443, 1545, 2153
TscAI CASTG 6 cut(s) 120, 510, 562, 597, 726, 1293
TseFI GTSAC 8 cut(s) 476, 829, 1284, 1649, 1706, 1867, 2014, 2037
TseI GCWGC 6 cut(s) 168, 191, 653, 863, 1100, 1538
Tsp45I GTSAC 8 cut(s) 476, 829, 1284, 1649, 1706, 1867, 2014, 2037
TspDTI ATGAA 8 cut(s) 42, 674, 752, 858, 1391, 1631, 1712, 2162
TspMI CCCGGG 1 cut(s) 561
TspRI CASTG 6 cut(s) 120, 510, 562, 597, 726, 1293
Van91I CCANNNNNTGG 1 cut(s) 1014
Vha464I CTTAAG 1 cut(s) 2152
VpaK11BI GGWCC 3 cut(s) 1070, 1610, 1926
XapI RAATTY 2 cut(s) 449, 1450
XceI RCATGY 2 cut(s) 366, 2164
XcmI CCANNNNNNNNNTGG 2 cut(s) 1669, 1891
XmaI CCCGGG 1 cut(s) 561
XmiI GTMKAC 1 cut(s) 1689
XspI CTAG 8 cut(s) 177, 462, 587, 905, 1109, 1296, 1601, 1968
ZraI GACGTC 1 cut(s) 229
ZrmI AGTACT 1 cut(s) 175
Zsp2I ATGCAT 1 cut(s) 1120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.