Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0005094

Physical Location & Seq

Genomic Coordinates Forward (+)

417280 .. 426777

Gene Structure 9498 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g00440.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2673 bp

ATGGTTAGTGGTAATCTGTTTCATTGTAGAAAGAATTCATGGCCTCCTCAGGAGTATATCCCCAGGAACACTTTGCAATTGTTTGACACTGATAGCGCTGCCCCACCAGAACAAGCCTGGAGGAGGAAGTTAAACAGCCATGCTAACATTCTTAAAGAGTTTAGTATTACATTTAGGGAAGCAATCAAAATGGTTAGGTTAGGTGTACGCTTGTGGTCATACGTCAGAGAAGAGGCTTCTCATGGAAGGAAAGCACCTATTGATCCTTTCACACGAGGCATTTGCAAGCCATCTGCATCTCAAGGGGTTCCACTCGGAGGGATGGGAAGTGGCAGCATCTCAAGAGGTTTTAGAGGCGAGTTTAGGCAATGGCAAATCATTCCGGGTGTATGTGATGGTTCTCCTGTCATGGCCAATCAATTCTCTATCTTTATATCTAGAGATGGAGGGAGTAAAAGCTATGCATCAGTCTTGGCCCCAGGTCAACATGATGGAATAGGAAAAGCTGGTGATCAGGGTATATCATCATGGGGTTGGAATCTGGGTGGTCAGCATTCCACGTACCATGCTCTATTTCCAAGGGCATGGACTGTATATGATGGTGAACCTGATCCAGAATTAAAAATTTCTTGTCGGCAAATATCACCATTTATTCCTCATAATTATAGAGATAGTAGTCTTCCTACAGCAGTTTTTGTCTACACGTTGGTGAACACTGGAAAAGAAAGAGCAAAAGTCAGCCTTCTATTTACATGGGCGAATTCAATTGGAGGAATCTCACATTTGTCGGGAGACCACGTCAATGAACCATTTATAGGCGAAGATGGAGTCTCTGGTGTACTTCTTCATCACAAGACTGCAAAAGGGAGTCCACCTGTTACTTTTGCAATAGCTGCATGTGAAACACAAAATGTAAGCGTTAGCGTGTTGCCATGTTTTGGTTTATCTGAAGAAAGTAGTGTTACGGCGAAAGAAATGTGGGACCAAATGGTGAAGGATGGACAATTTGATCGTGAGAATTTCAATTCTGGGCCATGCATGTCTTCCTCGCCTGGAGAGACACTCTGTGCTGCAGTGTCAGCTACTGCTTGGGTAGAACCCCATGGGAAGTGCACTGTCGCCTTTGGTCTATCCTGGTCATCCCCAAAAGTAAAATTTTTGAAGGGAAGCTCATATCCAAGGAGGTACACAAAATTCTATGGGACCTCTGAAAGGGCAGCTCAAGACTTGGTGCATGATGCACTAACAAATTATAAGCGATGGGAAGAAGAGATTGAAATTTGGCAAAATCCTATCCTCAAGGATGAAAAATTACCAGAATGGTACAAGTTCACGTTGTTCAATGAGCTCTACTTTTTGGTCGCTGGTGGAACGGTCTGGATTGATTCTCCCTCACTTGACAAAAAAATCAAGAAGAATCAGAGTCAATTAACAAATGGTGAGTATAACAAGGCCACTGAACATAAAGTGAACGGCAAAGTGGTCGAGGATACTGCAATGTTGGACCCACAAAAACATTATGAAGATGTGGGGAGGTTTCTTTATCTGGAAGGAGTTGAATACATCATGTGGAACACATATGACGTGCACTTCTATGCATCATTTGCCCTTCTTGATCTGTTTCCCAAAATTGAACTCAATATACAAAGGGACTTTGCAAAAGCAGTTTTATCTGAAGATGGAAGAAGAGTAAAGTTTCTGGCTGAGGGGAATTGGGGAATTCGCAAGGTCTATAGAGATTTTGCTGCGACAGGGGATATGTCATTTGGAGTTGATGTCTGGCCTGCTGTCCGCGCAGCAATGGAATACATGGAACAATTTGATAGGGACAATGATGGTCTCATTGAGAATGATGGTTTCCCTGACCAGACCTATGATGCCTGGACAGTTCATGGAGTAAGTGCTTACTGTGGTTGCTTGTGGCTTGCTGCACTTCAAGCTGCTGCAGCAATGGCCATTCAACTAGGTGATAAGGCCTTTGCTGAATGGTGCAAAACCAAATTTTTGAAGGCCAAACCAGCTTTTGAAGAAAAACTGTGGAATGGATCTTATTTTAATTATGATAGTGGATCTAGTAGTAACAGTAAATCTATACAAGCGGATCAACTGGCCGGACAGTGGTATACTGCATCTTCAGGTTTACCTTCACTTTTTGATGATGCTAAAGTCCAAAGTGCTTTGCAGAAAATCTATGATTTCAACGTCATGAAAGTTAAAGGAGGTAGGATGGGTGCTGTAAATGGAATGCACCCTAATGGTAGGGTGGATGAGTCTTGTATGCAATCTCGTGAAATATGGACGGGTGTCACTTATGGGGTGGCTGCCACAATGATCCTTGCTGGAAAGGAGAAAGAGGCATTCACCACTGCTGAAGGAATTTTTATTGCTGGCTGGTCTGAAGAGGGCTACGGATACGGGTTCCAGACTCCCGAGGGGTGGACGATGGATGGGCACTTCCGATCCCTGATATATATGAGGCCACTGTCTATATGGAGCATGCAGTGGGCATTATCAATGCCAAAGGCAATTCTTGAGGCTCCCAAGGCCAATGTAATGGACAGAATCCATATATCGTCTCTTAGTTCTCGATCCTCCCACAGCGAAACAGGTGTGAGAAAGATTGCAACCAAGGCAAAGTGCTTCAGTAATTCTGTGTTCAATTGTGCATGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0006082 GO:0006629 GO:0007275 GO:0007399 GO:0007417 GO:0008150 GO:0008152 GO:0008202 GO:0008206 GO:0008422 GO:0009056 GO:0009987 GO:0015926 GO:0016020 GO:0016021 GO:0016137 GO:0016139 GO:0016787 GO:0016798 GO:0019752 GO:0021953 GO:0021954 GO:0022008 GO:0030154 GO:0030182 GO:0031224 GO:0032501 GO:0032502 GO:0032787 GO:0043436 GO:0044237 GO:0044238 GO:0044281 GO:0044425 GO:0048468 GO:0048666 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0071704 GO:1901135 GO:1901136 GO:1901360 GO:1901575 GO:1901615 GO:1901657 GO:1901658

KEGG Pathways

Metabolic & Signaling

ko00511 ko00600 ko01100 map00511 map00600 map01100

Pfam Domains

Protein Families

DUF608 Glyco_hydr_116N

Protein Analysis

891

Amino Acids

99.15

Weight (kDa)

6.62

Isoelectric Point (pI)

43.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydr_116N	PF12215	102 - 421	5.1e-107	beta-glucosidase 2, glycosyl-hydrolase family 116 N-term
DUF608	PF04685	511 - 872	3.9e-162	Glycosyl-hydrolase family 116, catalytic region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0005094)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G24180 AT3G24180
fragaria_vesca	FvH4_4g00440 FvH4_4g00440 FvH4_4g00440 FvH4_4g00440 FvH4_4g00440
malus_domestica	MD04G1004800.v1.1
prunus_persica	Prupe.1G004700_v2.0.a1 Prupe.1G004700_v2.0.a1 Prupe.1G004700_v2.0.a1
pyrus_communis	pycom04g00480
rosa_chinensis	RchiOBHm_Chr4g0385861
rosa_laevigata	RLG00000010208
rosa_multiflora	Rmu_co8376727.1_g000001 Rmu_co8521537.1_g000001 Rmu_sc0004773.1_g000004 Rmu_sc0008728.1_g000010
rosa_roxburghii	Rroxscaffold_5G00333460
rosa_rugosa	Rorug01G0169800 Rorug03G0301400 Rorug03G0301500 Rorug03G0301600 Rorug03G0301700 Rorug05G0362900
rosa_samantha	Rh4AG006800 Rh4BG004300 Rh4CG007300 Rh4DG006300
rosa_wichuraiana	Rw4G000370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1254
AccB7I	CCANNNNNTGG	1 cut(s)	938
AccI	GTMKAC	2 cut(s)	699, 2123
AccII	CGCG	1 cut(s)	1794
AciI	CCGC	2 cut(s)	1792, 2099
AclWI	GGATC	8 cut(s)	257, 605, 2053, 2077, 2109, 2326, 2454, 2583
AcoI	YGGCCR	3 cut(s)	411, 1953, 2109
AcuI	CTGAAG	6 cut(s)	969, 1695, 2118, 2391, 2418, 2626
AdeI	CACNNNGTG	1 cut(s)	1067
AfaI	GTAC	5 cut(s)	207, 563, 840, 1187, 1325
AfeI	AGCGCT	1 cut(s)	97
AfiI	CCNNNNNNNGG	7 cut(s)	123, 317, 815, 938, 1212, 2118, 2436
AflIII	ACRYGT	1 cut(s)	702
AjiI	CACGTC	2 cut(s)	799, 1585
AjnI	CCWGG	6 cut(s)	62, 116, 478, 1051, 1133, 1880
AleI	CACNNNNGTG	1 cut(s)	707
AluBI	AGCT	9 cut(s)	459, 506, 893, 1082, 1170, 1220, 1348, 1940, 2021
AluI	AGCT	9 cut(s)	459, 506, 893, 1082, 1170, 1220, 1348, 1940, 2021
Alw21I	GWGCWC	3 cut(s)	1115, 1350, 1590
Alw26I	GTCTC	5 cut(s)	786, 835, 1052, 1844, 2580
Alw44I	GTGCAC	2 cut(s)	1111, 1586
AlwI	GGATC	8 cut(s)	257, 605, 2053, 2077, 2109, 2326, 2454, 2583
AlwNI	CAGNNNCTG	1 cut(s)	1085
Ama87I	CYCGRG	1 cut(s)	2429
Aor51HI	AGCGCT	1 cut(s)	97
ApaLI	GTGCAC	2 cut(s)	1111, 1586
ArsI	GACNNNNNNTTYG	2 cut(s)	813, 845
Asp700I	GAANNNNTTC	1 cut(s)	34
AspLEI	GCGC	2 cut(s)	98, 1796
AspS9I	GGNCC	5 cut(s)	475, 982, 1031, 1203, 1504
AsuC2I	CCSGG	1 cut(s)	384
AsuHPI	GGTGA	8 cut(s)	521, 614, 636, 721, 1003, 1451, 1979, 2353
AvaI	CYCGRG	1 cut(s)	2429
AvaII	GGWCC	3 cut(s)	982, 1203, 1504
AxyI	CCTNAGG	1 cut(s)	48
BaeGI	GKGCMC	3 cut(s)	1115, 1590, 2454
BalI	TGGCCA	2 cut(s)	413, 1955
BanII	GRGCYC	1 cut(s)	1350
BauI	CACGAG	2 cut(s)	273, 2286
BbsI	GAAGAC	2 cut(s)	671, 1035
Bbv12I	GWGCWC	3 cut(s)	1115, 1350, 1590
BbvCI	CCTCAGC	1 cut(s)	1704
BceAI	ACGGC	2 cut(s)	981, 1489
BcgI	CGANNNNNNTGC	6 cut(s)	264, 298, 1465, 1475, 1499, 1509
BciT130I	CCWGG	6 cut(s)	64, 118, 480, 1053, 1135, 1882
BciVI	GTATCC	2 cut(s)	1483, 2405
BclI	TGATCA	1 cut(s)	511
BcnI	CCSGG	1 cut(s)	384
BcoDI	GTCTC	5 cut(s)	786, 835, 1052, 1844, 2580
BfaI	CTAG	3 cut(s)	438, 1964, 2073
BfmI	CTRYAG	4 cut(s)	684, 1071, 1732, 1944
BfoI	RGCGCY	1 cut(s)	99
BfuI	GTATCC	2 cut(s)	1483, 2405
Bme1390I	CCNGG	7 cut(s)	64, 118, 384, 480, 1053, 1135, 1882
Bme18I	GGWCC	3 cut(s)	982, 1203, 1504
BmeT110I	CYCGRG	1 cut(s)	2429
BmgBI	CACGTC	2 cut(s)	799, 1585
BmgT120I	GGNCC	5 cut(s)	475, 982, 1031, 1203, 1504
BmiI	GGNNCC	7 cut(s)	309, 477, 983, 1204, 1506, 2420, 2538
BmrFI	CCNGG	7 cut(s)	64, 118, 384, 480, 1053, 1135, 1882
BmsI	GCATC	8 cut(s)	305, 345, 473, 1228, 1607, 1867, 2138, 2149
BoxI	GACNNNNGTC	1 cut(s)	2303
BpiI	GAAGAC	2 cut(s)	671, 1035
BplI	GAGNNNNNCTC	2 cut(s)	1047, 1079
BpmI	CTGGAG	2 cut(s)	139, 1074
Bpu10I	CCTNAGC	1 cut(s)	1704
BpuEI	CTTGAG	5 cut(s)	285, 325, 1206, 1283, 2552
BpuMI	CCSGG	1 cut(s)	384
BsaAI	YACGTR	1 cut(s)	561
BsaI	GGTCTC	2 cut(s)	786, 1844
BsaJI	CCNNGG	8 cut(s)	62, 478, 578, 1102, 1178, 2430, 2541, 2628
BsaXI	ACNNNNNCTCC	4 cut(s)	783, 813, 1761, 1791
Bsc4I	CCNNNNNNNGG	7 cut(s)	123, 317, 815, 938, 1212, 2118, 2436
Bse1I	ACTGG	2 cut(s)	721, 2112
Bse21I	CCTNAGG	1 cut(s)	48
Bse3DI	GCAATG	4 cut(s)	374, 1503, 1806, 1956
BseBI	CCWGG	6 cut(s)	64, 118, 480, 1053, 1135, 1882
BseDI	CCNNGG	8 cut(s)	62, 478, 578, 1102, 1178, 2430, 2541, 2628
BseGI	GGATG	7 cut(s)	327, 1003, 1139, 1309, 2232, 2272, 2452
BseLI	CCNNNNNNNGG	7 cut(s)	123, 317, 815, 938, 1212, 2118, 2436
BseMI	GCAATG	4 cut(s)	374, 1503, 1806, 1956
BseMII	CTCAG	2 cut(s)	62, 1695
BseNI	ACTGG	2 cut(s)	721, 2112
BseRI	GAGGAG	2 cut(s)	36, 136
BseSI	GKGCMC	3 cut(s)	1115, 1590, 2454
BsgI	GTGCAG	1 cut(s)	1914
Bsh1236I	CGCG	1 cut(s)	1794
BsiHKAI	GWGCWC	3 cut(s)	1115, 1350, 1590
BsiHKCI	CYCGRG	1 cut(s)	2429
BsiSI	CCGG	2 cut(s)	383, 2112
BslFI	GGGAC	4 cut(s)	995, 1216, 1664, 1841
BslI	CCNNNNNNNGG	7 cut(s)	123, 317, 815, 938, 1212, 2118, 2436
BsmAI	GTCTC	5 cut(s)	786, 835, 1052, 1844, 2580
BsmBI	CGTCTC	1 cut(s)	2580
BsmFI	GGGAC	4 cut(s)	995, 1216, 1664, 1841
BsmI	GAATGC	3 cut(s)	553, 2250, 2357
Bso31I	GGTCTC	2 cut(s)	786, 1844
BsoBI	CYCGRG	1 cut(s)	2429
Bsp1286I	GDGCHC	4 cut(s)	1115, 1350, 1590, 2454
Bsp19I	CCATGG	1 cut(s)	1102
BspACI	CCGC	2 cut(s)	1792, 2099
BspCNI	CTCAG	2 cut(s)	61, 1696
BspFNI	CGCG	1 cut(s)	1794
BspHI	TCATGA	1 cut(s)	2205
BspLI	GGNNCC	7 cut(s)	309, 477, 983, 1204, 1506, 2420, 2538
BspMAI	CTGCAG	2 cut(s)	1075, 1948
BspPI	GGATC	8 cut(s)	257, 605, 2053, 2077, 2109, 2326, 2454, 2583
BspTNI	GGTCTC	2 cut(s)	786, 1844
BsrDI	GCAATG	4 cut(s)	374, 1503, 1806, 1956
BsrI	ACTGG	2 cut(s)	721, 2112
BssECI	CCNNGG	8 cut(s)	62, 478, 578, 1102, 1178, 2430, 2541, 2628
BssNAI	GTATAC	1 cut(s)	2124
BssSI	CACGAG	2 cut(s)	273, 2286
BssT1I	CCWWGG	5 cut(s)	578, 1102, 1178, 2541, 2628
Bst1107I	GTATAC	1 cut(s)	2124
Bst2BI	CACGAG	2 cut(s)	273, 2286
Bst2UI	CCWGG	6 cut(s)	64, 118, 480, 1053, 1135, 1882
Bst4CI	ACNGT	9 cut(s)	592, 1117, 1375, 1888, 1910, 2037, 2084, 2118, 2484
Bst6I	CTCTTC	4 cut(s)	225, 1263, 1681, 2394
BstAPI	GCANNNNNTGC	2 cut(s)	893, 1604
BstBAI	YACGTR	1 cut(s)	561
BstC8I	GCNNGC	6 cut(s)	287, 1785, 1926, 2389, 2498, 2668
BstDEI	CTNAG	3 cut(s)	48, 1704, 2579
BstDSI	CCRYGG	1 cut(s)	1102
BstENI	CCTNNNNNAGG	2 cut(s)	121, 1210
BstF5I	GGATG	7 cut(s)	327, 1003, 1139, 1309, 2232, 2272, 2452
BstFNI	CGCG	1 cut(s)	1794
BstH2I	RGCGCY	1 cut(s)	99
BstHHI	GCGC	2 cut(s)	98, 1796
BstMAI	GTCTC	5 cut(s)	786, 835, 1052, 1844, 2580
BstNI	CCWGG	6 cut(s)	64, 118, 480, 1053, 1135, 1882
BstNSI	RCATGY	4 cut(s)	900, 1042, 2500, 2670
BstPAI	GACNNNNGTC	1 cut(s)	2303
BstSCI	CCNGG	7 cut(s)	62, 116, 382, 478, 1051, 1133, 1880
BstSFI	CTRYAG	4 cut(s)	684, 1071, 1732, 1944
BstSLI	GKGCMC	3 cut(s)	1115, 1590, 2454
BstUI	CGCG	1 cut(s)	1794
BstV2I	GAAGAC	2 cut(s)	671, 1035
BstX2I	RGATCY	2 cut(s)	2045, 2069
BstXI	CCANNNNNNTGG	2 cut(s)	585, 2554
BstYI	RGATCY	2 cut(s)	2045, 2069
BstZ17I	GTATAC	1 cut(s)	2124
Bsu36I	CCTNAGG	1 cut(s)	48
BsuI	GTATCC	2 cut(s)	1483, 2405
BtgI	CCRYGG	1 cut(s)	1102
BtgZI	GCGATG	1 cut(s)	1273
BtrI	CACGTC	2 cut(s)	799, 1585
BtsCI	GGATG	7 cut(s)	327, 1003, 1139, 1309, 2232, 2272, 2452
BtsI	GCAGTG	3 cut(s)	1080, 2364, 2507
BtsIMutI	CAGTG	9 cut(s)	87, 714, 1080, 1113, 1455, 2123, 2364, 2480, 2507
Cac8I	GCNNGC	6 cut(s)	287, 1785, 1926, 2389, 2498, 2668
CaiI	CAGNNNCTG	1 cut(s)	1085
CciI	TCATGA	1 cut(s)	2205
CfoI	GCGC	2 cut(s)	98, 1796
Cfr13I	GGNCC	5 cut(s)	475, 982, 1031, 1203, 1504
Csp6I	GTAC	5 cut(s)	206, 562, 839, 1186, 1324
CviQI	GTAC	5 cut(s)	206, 562, 839, 1186, 1324
DdeI	CTNAG	3 cut(s)	48, 1704, 2579
DraIII	CACNNNGTG	1 cut(s)	1067
EaeI	YGGCCR	3 cut(s)	411, 1953, 2109
Eam1104I	CTCTTC	4 cut(s)	225, 1263, 1681, 2394
EarI	CTCTTC	4 cut(s)	225, 1263, 1681, 2394
Ecl136II	GAGCTC	1 cut(s)	1348
Eco130I	CCWWGG	5 cut(s)	578, 1102, 1178, 2541, 2628
Eco147I	AGGCCT	1 cut(s)	1976
Eco24I	GRGCYC	1 cut(s)	1350
Eco31I	GGTCTC	2 cut(s)	786, 1844
Eco47I	GGWCC	3 cut(s)	982, 1203, 1504
Eco47III	AGCGCT	1 cut(s)	97
Eco53kI	GAGCTC	1 cut(s)	1348
Eco57I	CTGAAG	6 cut(s)	969, 1695, 2118, 2391, 2418, 2626
Eco81I	CCTNAGG	1 cut(s)	48
Eco88I	CYCGRG	1 cut(s)	2429
EcoICRI	GAGCTC	1 cut(s)	1348
EcoNI	CCTNNNNNAGG	2 cut(s)	121, 1210
EcoO109I	RGGNCCY	1 cut(s)	1203
EcoRI	GAATTC	3 cut(s)	34, 760, 1719
EcoRII	CCWGG	6 cut(s)	62, 116, 478, 1051, 1133, 1880
EcoT14I	CCWWGG	5 cut(s)	578, 1102, 1178, 2541, 2628
EcoT22I	ATGCAT	3 cut(s)	466, 1040, 1600
EcoT38I	GRGCYC	1 cut(s)	1350
ErhI	CCWWGG	5 cut(s)	578, 1102, 1178, 2541, 2628
Esp3I	CGTCTC	1 cut(s)	2580
FalI	AAGNNNNNCTT	2 cut(s)	726, 758
FaqI	GGGAC	4 cut(s)	995, 1216, 1664, 1841
FauNDI	CATATG	1 cut(s)	1579
FbaI	TGATCA	1 cut(s)	511
FblI	GTMKAC	2 cut(s)	699, 2123
FokI	GGATG	7 cut(s)	334, 1010, 1126, 1316, 2239, 2279, 2459
FriOI	GRGCYC	1 cut(s)	1350
FspBI	CTAG	3 cut(s)	438, 1964, 2073
GlaI	GCGC	2 cut(s)	97, 1795
GsuI	CTGGAG	2 cut(s)	139, 1074
HaeII	RGCGCY	1 cut(s)	99
HapII	CCGG	2 cut(s)	383, 2112
HhaI	GCGC	2 cut(s)	98, 1796
Hin6I	GCGC	2 cut(s)	96, 1794
HinP1I	GCGC	2 cut(s)	96, 1794
HincII	GTYRAC	1 cut(s)	485
HindII	GTYRAC	1 cut(s)	485
HpaII	CCGG	2 cut(s)	383, 2112
HphI	GGTGA	8 cut(s)	521, 614, 636, 721, 1003, 1451, 1979, 2353
Hpy188I	TCNGA	8 cut(s)	227, 317, 949, 1210, 1422, 1675, 2398, 2459
HpyAV	CCTTC	9 cut(s)	240, 752, 988, 1156, 1544, 1619, 2002, 2154, 2366
HpyCH4III	ACNGT	9 cut(s)	592, 1117, 1375, 1888, 1910, 2037, 2084, 2118, 2484
HpyCH4IV	ACGT	7 cut(s)	222, 560, 704, 798, 1334, 1584, 2202
HpyF3I	CTNAG	3 cut(s)	48, 1704, 2579
HpySE526I	ACGT	7 cut(s)	222, 560, 704, 798, 1334, 1584, 2202
HspAI	GCGC	2 cut(s)	96, 1794
Ksp22I	TGATCA	1 cut(s)	511
LmnI	GCTCC	2 cut(s)	2493, 2542
LweI	GCATC	8 cut(s)	305, 345, 473, 1228, 1607, 1867, 2138, 2149
MaeI	CTAG	3 cut(s)	438, 1964, 2073
MaeII	ACGT	7 cut(s)	222, 560, 704, 798, 1334, 1584, 2202
MaeIII	GTNAC	4 cut(s)	877, 961, 2078, 2305
MfeI	CAATTG	3 cut(s)	77, 765, 2659
MflI	RGATCY	2 cut(s)	2045, 2069
MhlI	GDGCHC	4 cut(s)	1115, 1350, 1590, 2454
MlsI	TGGCCA	2 cut(s)	413, 1955
MluNI	TGGCCA	2 cut(s)	413, 1955
MlyI	GAGTC	5 cut(s)	837, 877, 1432, 2279, 2419
MmeI	TCCRAC	2 cut(s)	515, 1482
Mox20I	TGGCCA	2 cut(s)	413, 1955
Mph1103I	ATGCAT	3 cut(s)	466, 1040, 1600
MroXI	GAANNNNTTC	1 cut(s)	34
MscI	TGGCCA	2 cut(s)	413, 1955
MseI	TTAA	6 cut(s)	131, 153, 620, 1430, 2055, 2214
MslI	CAYNNNNRTG	4 cut(s)	707, 801, 1593, 2253
Msp20I	TGGCCA	2 cut(s)	413, 1955
MspI	CCGG	2 cut(s)	383, 2112
MspR9I	CCNGG	7 cut(s)	64, 118, 384, 480, 1053, 1135, 1882
MunI	CAATTG	3 cut(s)	77, 765, 2659
Mva1269I	GAATGC	3 cut(s)	553, 2250, 2357
MvaI	CCWGG	6 cut(s)	64, 118, 480, 1053, 1135, 1882
MvnI	CGCG	1 cut(s)	1794
NciI	CCSGG	1 cut(s)	384
NcoI	CCATGG	1 cut(s)	1102
NdeI	CATATG	1 cut(s)	1579
NlaIV	GGNNCC	7 cut(s)	309, 477, 983, 1204, 1506, 2420, 2538
NmuCI	GTSAC	1 cut(s)	2305
NsiI	ATGCAT	3 cut(s)	466, 1040, 1600
NspI	RCATGY	4 cut(s)	900, 1042, 2500, 2670
OliI	CACNNNNGTG	1 cut(s)	707
PaeI	GCATGC	2 cut(s)	2500, 2670
PagI	TCATGA	1 cut(s)	2205
PceI	AGGCCT	1 cut(s)	1976
PctI	GAATGC	3 cut(s)	553, 2250, 2357
PdmI	GAANNNNTTC	1 cut(s)	34
PfeI	GAWTC	5 cut(s)	538, 774, 1385, 1417, 2562
PflFI	GACNNNGTC	1 cut(s)	797
PflMI	CCANNNNNTGG	1 cut(s)	938
PleI	GAGTC	5 cut(s)	836, 876, 1431, 2278, 2419
PpsI	GAGTC	5 cut(s)	836, 876, 1431, 2278, 2419
Ppu21I	YACGTR	1 cut(s)	561
PpuMI	RGGWCCY	1 cut(s)	1203
PshAI	GACNNNNGTC	1 cut(s)	2303
PsiI	TTATAA	1 cut(s)	1254
Psp124BI	GAGCTC	1 cut(s)	1350
Psp5II	RGGWCCY	1 cut(s)	1203
Psp6I	CCWGG	6 cut(s)	62, 116, 478, 1051, 1133, 1880
PspGI	CCWGG	6 cut(s)	62, 116, 478, 1051, 1133, 1880
PspN4I	GGNNCC	7 cut(s)	309, 477, 983, 1204, 1506, 2420, 2538
PspPI	GGNCC	5 cut(s)	475, 982, 1031, 1203, 1504
PspPPI	RGGWCCY	1 cut(s)	1203
PsrI	GAACNNNNNNTAC	2 cut(s)	1626, 1658
PstI	CTGCAG	2 cut(s)	1075, 1948
PstNI	CAGNNNCTG	1 cut(s)	1085
PsuI	RGATCY	2 cut(s)	2045, 2069
PsyI	GACNNNGTC	1 cut(s)	797
RsaI	GTAC	5 cut(s)	207, 563, 840, 1187, 1325
RsaNI	GTAC	5 cut(s)	206, 562, 839, 1186, 1324
RseI	CAYNNNNRTG	4 cut(s)	707, 801, 1593, 2253
SacI	GAGCTC	1 cut(s)	1350
SaqAI	TTAA	6 cut(s)	131, 153, 620, 1430, 2055, 2214
Sau96I	GGNCC	5 cut(s)	475, 982, 1031, 1203, 1504
SchI	GAGTC	5 cut(s)	837, 877, 1432, 2279, 2419
ScrFI	CCNGG	7 cut(s)	64, 118, 384, 480, 1053, 1135, 1882
SduI	GDGCHC	4 cut(s)	1115, 1350, 1590, 2454
SfaNI	GCATC	8 cut(s)	305, 345, 473, 1228, 1607, 1867, 2138, 2149
SfcI	CTRYAG	4 cut(s)	684, 1071, 1732, 1944
SinI	GGWCC	3 cut(s)	982, 1203, 1504
SmiMI	CAYNNNNRTG	4 cut(s)	707, 801, 1593, 2253
SmlI	CTYRAG	5 cut(s)	300, 340, 1221, 1298, 2531
SmoI	CTYRAG	5 cut(s)	300, 340, 1221, 1298, 2531
SphI	GCATGC	2 cut(s)	2500, 2670
SseBI	AGGCCT	1 cut(s)	1976
SsiI	CCGC	2 cut(s)	1792, 2099
SspMI	CTAG	3 cut(s)	438, 1964, 2073
SstI	GAGCTC	1 cut(s)	1350
StuI	AGGCCT	1 cut(s)	1976
StyD4I	CCNGG	7 cut(s)	62, 116, 382, 478, 1051, 1133, 1880
StyI	CCWWGG	5 cut(s)	578, 1102, 1178, 2541, 2628
TaaI	ACNGT	9 cut(s)	592, 1117, 1375, 1888, 1910, 2037, 2084, 2118, 2484
TaiI	ACGT	7 cut(s)	225, 563, 707, 801, 1337, 1587, 2205
TaqI	TCGA	2 cut(s)	1485, 2587
TatI	WGTACW	1 cut(s)	838
TfiI	GAWTC	5 cut(s)	538, 774, 1385, 1417, 2562
Tru1I	TTAA	6 cut(s)	131, 153, 620, 1430, 2055, 2214
Tru9I	TTAA	6 cut(s)	131, 153, 620, 1430, 2055, 2214
TscAI	CASTG	9 cut(s)	94, 721, 1080, 1120, 1462, 2123, 2371, 2487, 2507
TseFI	GTSAC	1 cut(s)	2305
Tsp45I	GTSAC	1 cut(s)	2305
TspDTI	ATGAA	8 cut(s)	11, 27, 819, 836, 1320, 1536, 1880, 2222
TspGWI	ACGGA	1 cut(s)	2424
TspRI	CASTG	9 cut(s)	94, 721, 1080, 1120, 1462, 2123, 2371, 2487, 2507
Tth111I	GACNNNGTC	1 cut(s)	797
Van91I	CCANNNNNTGG	1 cut(s)	938
VneI	GTGCAC	2 cut(s)	1111, 1586
VpaK11BI	GGWCC	3 cut(s)	982, 1203, 1504
XagI	CCTNNNNNAGG	2 cut(s)	121, 1210
XbaI	TCTAGA	1 cut(s)	437
XceI	RCATGY	4 cut(s)	900, 1042, 2500, 2670
XcmI	CCANNNNNNNNNTGG	2 cut(s)	114, 2487
XmiI	GTMKAC	2 cut(s)	699, 2123
XmnI	GAANNNNTTC	1 cut(s)	34
XspI	CTAG	3 cut(s)	438, 1964, 2073
Zsp2I	ATGCAT	3 cut(s)	466, 1040, 1600

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.