Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0005094

Physical Location & Seq

Genomic Coordinates Forward (+)

601727 .. 612258

Gene Structure 10532 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1004800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2820 bp

ATGGTTAGTGGGCATTTGTTCCATTGTAGAAAGAATTCATGGCCGCCTGAGGAGTATATCAGCAAAAACACTTTGCACCTGTTTGATTTTGATAGTGCTGCCCCACCAGAACATGCCTGGAGGAAGAAGTTAAACAGCCAGGCTAATCTTCTCAGAGAATTTAGTGTTACATTTAGGGAAGCGATAAAGATGGTTCGGTTAGGTATACGCCTGTGGTCATACATCAGGGAAGAGGCTTCTCACGGAAGGAAAGCACCTATTGACCCTTTTACTCGAGAAAGTTGCAAGCCATCTGCATCTCAGGGGGTTCCACTTGGAGGGATGGGAAGTGGCAGCATATCTAGAGGTTTTAGAGGCGAGTTCAGGCAATGGCAAATCATTCCTGGTATCTGCAAAGGTTCTCCTGTCATGGCCAATCAGTTCTCTATTTTTATATCTAGAGAAGGAACGAATAAAAATTATGCATCAGTCTTGGCCCCCGGTCATCATGAAGGATTAGGAAAAGCTGATGATGAGGGTATATCATCATGGGGATGGAACCTGGGTGGCCAGCATTCAACATACCATGCACTATTTCCAAGGGCATGGACCATATATGATGGTGAACCTGACCCAGAATTGAAAATTTCTTGTCGGCAAATATCACCATTCATCCCTCATAATTATAGAGAAAGTAGTCTTCCTACAGCCGTTTTTGTTTACACGTTGGTGAACACTGGAAAAGAAAGAGCAAAAGTCAGCCTCCTATTTACATGGGCGAATTCAATTGGAGGAGTCTCACACTTGTCGGGCGATCATGTGAACGAACCATTTATTGGCGAAGATGGAGTCTCTGGTGTACTTCTACATCACAAGACTGCAAAAGGGAATCCTCCTGTTACATTTGGAATAGCTGCGTGTGAAACTCAAAATGTAAGCGTGAGTGTGTTGCCGTGTTTTGGGTTATCTGAAGGAAGTACCACTACGGCGAAAGAAATGTGGAATAAAATGGTGCAGGATGGGCAATTTGATCGTGATAATTTCAATTCTGGACCATGCATGTCTTCATCACCTGGAGAAACACTCTGTGCTGCAGTAGCAGCTACTGCTTGGGTGGAACCTCATGGGAAGTGCACCGTTGCATTTGGCCTATCATGGTCATCTCCAAAAGTAAAATTTTTGAAGGGAAGCTCATACCATAGGAGGTACACAAAATTCTACGGGACCTCTGAAAGGGCAGCTCAGGATTTGGTGCATGATGCGCTGACAAATTATAAGCGATGGGAAGAAGATATTGAAAGGTGGCAAAGCCCTATCCTCAGGGATGAAACATTACCAGAATGGTACAAGTTCACATTATTCAATGAGCTCTACTTTTTGGTGGCTGGTGGGACAGTTTGGATCGATTCTCCCTTGCCTGCAACAAACATTACAAACAATCGACACCAATTGACAAATGTTGAAAATACAGATGTTAAGGTTAGTGAAGCTGAAGTAAATAGCAATCACAACGCAGCTGTTGAGCACACTGCAACTGGTTGCCAAAGGAGTGTACATTTGGACCCACAAAATGCCAGTGAAGATGTTGGGAGGTTCCTCTATCTGGAAGGAGTGGAATATATCATGTGGAACACATATGACGTACACTTTTATGCATCATTTGCCCTTCTCGAGCTGTTTCCCAAAATTGAACTTAATATTCAAAGGGACTTTGCAAAAGCCGTCTTATCAGAAGATGGAAGAAAAGTAAAGTTTCTTGCAGAGGGTAACTGGGGAATTCGTAAGGTTAGAGGAGCTGTCCCTCATGATCTGGGAACACACGATCCCTGGAACGAAATGAATGCATATAATATTCATGATACTAGTAAGTGGAAGGATTTGAACCCAAAGTTTGTGCTTCAGGTGTATAGAGATTTTTCTGCAACAGGGGATATGTCATTTGGAGTAGATGTTTGGCCTGCTGTTCGTGCTGCAATGGAGTACATGGAACAATTTGACCGAGACAATGATGGTCTCATTGAAAATGATGGTTTCCCTGATCAGACCTACGATGCTTGGACAGTTCATGGAGTAAGTGCTTACTGTGGGTGCTTGTGGCTAGCTGCACTTCAAGCTGCAGCCGCAATGGCCCTTCAACTAGGCGACAAGGCCTTTGCCGAATGGTGCAAAGCCAAATTTTTAAAGGCAAAACCATCTTTTGAGGAAAAATTGTGGAATGGTTCTTATTTTAATTACGATAGTGGATCAAGTAGTAACAGTAAGTCTATACAAGCAGATCAACTGGCTGGGCAGTGGTATACGGCGTCTTCTGGTTTACCTTCACTTTTTGATGATTTTAAAGTCGAAAGCTCTCTGCAGAAAATCTATGATTTCAATGTCATGAAAGTTAAAGGAGGAAAAATGGGTGCCGTAAATGGAATGCATCCCAATGGGAAGGTGGATGAGTCTTGCATGCAGTCACGTGAAATATGGACTGGTGTCACATATGGGGTGGCTGCTCTGATGATTCTTGCTGGAAAGGAGAAAGAGGCTTTCGCTACTGCTGAAGGTATTTTTATTGCCGGCTGGTCTGAAGATGGATACGCTTTCCAGACGCCCGAAGGATGGACGATGGACGGCCACTTCAGATCTCTAATATACATGAGGCCACTATCTATTTGGGGGATGCAATTCGCGTTGAACATGCCCAAGGCAATTCTCGAGGCTCCTCAGATCAACATAATGGACAGAATACATTTATCGTCTGTCAGTTCACGATCCTCTCACAGCGAAACAGGTGTGAGAAAGATTGCAACCAAGGCAAAGTGCTTTGGAGATTCTGTTTTTAACTGTGCATGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0006082 GO:0006629 GO:0007275 GO:0007399 GO:0007417 GO:0008150 GO:0008152 GO:0008202 GO:0008206 GO:0008422 GO:0009056 GO:0009987 GO:0015926 GO:0016020 GO:0016021 GO:0016137 GO:0016139 GO:0016787 GO:0016798 GO:0019752 GO:0021953 GO:0021954 GO:0022008 GO:0030154 GO:0030182 GO:0031224 GO:0032501 GO:0032502 GO:0032787 GO:0043436 GO:0044237 GO:0044238 GO:0044281 GO:0044425 GO:0048468 GO:0048666 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0071704 GO:1901135 GO:1901136 GO:1901360 GO:1901575 GO:1901615 GO:1901657 GO:1901658

KEGG Pathways

Metabolic & Signaling

ko00511 ko00600 ko01100 map00511 map00600 map01100

Pfam Domains

Protein Families

DUF608 Glyco_hydr_116N

Protein Analysis

940

Amino Acids

104.76

Weight (kDa)

6.17

Isoelectric Point (pI)

42.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydr_116N	PF12215	102 - 421	1.2e-108	beta-glucosidase 2, glycosyl-hydrolase family 116 N-term
DUF608	PF04685	523 - 882	8.5e-162	Glycosyl-hydrolase family 116, catalytic region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0005094)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G24180 AT3G24180
fragaria_vesca	FvH4_4g00440 FvH4_4g00440 FvH4_4g00440 FvH4_4g00440 FvH4_4g00440
malus_domestica	MD04G1004800.v1.1
prunus_persica	Prupe.1G004700_v2.0.a1 Prupe.1G004700_v2.0.a1 Prupe.1G004700_v2.0.a1
pyrus_communis	pycom04g00480
rosa_chinensis	RchiOBHm_Chr4g0385861
rosa_laevigata	RLG00000010208
rosa_multiflora	Rmu_co8376727.1_g000001 Rmu_co8521537.1_g000001 Rmu_sc0004773.1_g000004 Rmu_sc0008728.1_g000010
rosa_roxburghii	Rroxscaffold_5G00333460
rosa_rugosa	Rorug01G0169800 Rorug03G0301400 Rorug03G0301500 Rorug03G0301600 Rorug03G0301700 Rorug05G0362900
rosa_samantha	Rh4AG006800 Rh4BG004300 Rh4CG007300 Rh4DG006300
rosa_wichuraiana	Rw4G000370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1254
AccB1I	GGYRCC	1 cut(s)	2384
AccB7I	CCANNNNNTGG	1 cut(s)	815
AccI	GTMKAC	2 cut(s)	205, 2276
AccII	CGCG	1 cut(s)	2654
AciI	CCGC	2 cut(s)	44, 2100
AclWI	GGATC	4 cut(s)	1388, 1796, 2230, 2730
AcoI	YGGCCR	4 cut(s)	41, 411, 547, 2596
AcsI	RAATTY	8 cut(s)	34, 158, 624, 760, 1154, 1193, 1755, 2153
AcuI	CTGAAG	6 cut(s)	969, 1491, 1862, 2544, 2571, 2587
AcvI	CACGTG	1 cut(s)	2441
AcyI	GRCGYC	2 cut(s)	2282, 2573
AdeI	CACNNNGTG	1 cut(s)	1067
AfaI	GTAC	7 cut(s)	840, 958, 1187, 1325, 1533, 1623, 1961
AfiI	CCNNNNNNNGG	6 cut(s)	317, 815, 938, 1212, 1582, 2583
AflIII	ACRYGT	1 cut(s)	702
AhlI	ACTAGT	1 cut(s)	1841
AjnI	CCWGG	6 cut(s)	116, 138, 382, 540, 1051, 1805
AjuI	GAANNNNNNNTTGG	2 cut(s)	798, 830
AleI	CACNNNNGTG	1 cut(s)	707
AloI	GAACNNNNNNTCC	2 cut(s)	1786, 1818
Alw21I	GWGCWC	3 cut(s)	1115, 1350, 1506
Alw26I	GTCTC	4 cut(s)	781, 835, 1974, 1997
Alw44I	GTGCAC	1 cut(s)	1111
AlwI	GGATC	4 cut(s)	1388, 1796, 2230, 2730
AlwNI	CAGNNNCTG	1 cut(s)	1085
Ama87I	CYCGRG	3 cut(s)	273, 1649, 2678
ApaLI	GTGCAC	1 cut(s)	1111
ApoI	RAATTY	8 cut(s)	34, 158, 624, 760, 1154, 1193, 1755, 2153
ArsI	GACNNNNNNTTYG	4 cut(s)	813, 845, 2114, 2146
Asp700I	GAANNNNTTC	1 cut(s)	34
AspLEI	GCGC	1 cut(s)	1243
AspS9I	GGNCC	6 cut(s)	475, 588, 1031, 1203, 1540, 2107
AsuC2I	CCSGG	1 cut(s)	480
AsuHPI	GGTGA	4 cut(s)	614, 636, 721, 1041
AsuNHI	GCTAGC	1 cut(s)	2077
AvaI	CYCGRG	3 cut(s)	273, 1649, 2678
AvaII	GGWCC	4 cut(s)	588, 1031, 1203, 1540
AxyI	CCTNAGG	2 cut(s)	48, 1298
BaeGI	GKGCMC	1 cut(s)	1115
BalI	TGGCCA	2 cut(s)	413, 549
BanI	GGYRCC	1 cut(s)	2384
BanII	GRGCYC	1 cut(s)	1350
BarI	GAAGNNNNNNTAC	2 cut(s)	946, 978
BbrPI	CACGTG	1 cut(s)	2441
BbsI	GAAGAC	3 cut(s)	671, 1035, 2277
Bbv12I	GWGCWC	3 cut(s)	1115, 1350, 1506
BceAI	ACGGC	7 cut(s)	674, 916, 981, 1685, 2295, 2372, 2611
BcgI	CGANNNNNNTGC	6 cut(s)	264, 298, 992, 1026, 1802, 1836
BciT130I	CCWGG	6 cut(s)	118, 140, 384, 542, 1053, 1807
BciVI	GTATCC	1 cut(s)	2552
BclI	TGATCA	1 cut(s)	2017
BcnI	CCSGG	1 cut(s)	480
BcoDI	GTCTC	4 cut(s)	781, 835, 1974, 1997
BcuI	ACTAGT	1 cut(s)	1841
BfaI	CTAG	5 cut(s)	342, 438, 1842, 2078, 2117
BfmI	CTRYAG	4 cut(s)	684, 1071, 2094, 2333
BfuI	GTATCC	1 cut(s)	2552
BglI	GCCNNNNNGGC	1 cut(s)	2105
BglII	AGATCT	1 cut(s)	2606
Bme1390I	CCNGG	7 cut(s)	118, 140, 384, 480, 542, 1053, 1807
Bme18I	GGWCC	4 cut(s)	588, 1031, 1203, 1540
BmeT110I	CYCGRG	3 cut(s)	273, 1649, 2678
BmgT120I	GGNCC	6 cut(s)	475, 588, 1031, 1203, 1540, 2107
BmiI	GGNNCC	9 cut(s)	309, 477, 539, 1098, 1204, 1542, 1574, 2386, 2685
BmrFI	CCNGG	7 cut(s)	118, 140, 384, 480, 542, 1053, 1807
BmrI	ACTGGG	1 cut(s)	1759
BmsI	GCATC	7 cut(s)	305, 473, 1228, 1643, 2020, 2410, 2634
BmtI	GCTAGC	1 cut(s)	2081
BmuI	ACTGGG	1 cut(s)	1759
BoxI	GACNNNNGTC	1 cut(s)	2456
BpiI	GAAGAC	3 cut(s)	671, 1035, 2277
BplI	GAGNNNNNCTC	2 cut(s)	1047, 1079
BpmI	CTGGAG	2 cut(s)	139, 1074
Bpu10I	CCTNAGC	1 cut(s)	1221
BpuMI	CCSGG	1 cut(s)	480
Bsa29I	ATCGAT	1 cut(s)	1383
BsaAI	YACGTR	1 cut(s)	2441
BsaHI	GRCGYC	2 cut(s)	2282, 2573
BsaI	GGTCTC	1 cut(s)	1997
BsaJI	CCNNGG	6 cut(s)	478, 541, 578, 1805, 2667, 2775
BsaXI	ACNNNNNCTCC	4 cut(s)	1914, 1944, 2784, 2814
Bsc4I	CCNNNNNNNGG	6 cut(s)	317, 815, 938, 1212, 1582, 2583
Bse118I	RCCGGY	1 cut(s)	2540
Bse1I	ACTGG	6 cut(s)	721, 1519, 1554, 1754, 2265, 2458
Bse21I	CCTNAGG	2 cut(s)	48, 1298
Bse3DI	GCAATG	3 cut(s)	374, 1959, 2109
BseBI	CCWGG	6 cut(s)	118, 140, 384, 542, 1053, 1807
BseCI	ATCGAT	1 cut(s)	1383
BseDI	CCNNGG	6 cut(s)	478, 541, 578, 1805, 2667, 2775
BseGI	GGATG	9 cut(s)	327, 539, 651, 1003, 1309, 2401, 2425, 2588, 2649
BseLI	CCNNNNNNNGG	6 cut(s)	317, 815, 938, 1212, 1582, 2583
BseMI	GCAATG	3 cut(s)	374, 1959, 2109
BseMII	CTCAG	6 cut(s)	39, 166, 314, 1235, 1312, 2702
BseNI	ACTGG	6 cut(s)	721, 1519, 1554, 1754, 2265, 2458
BseRI	GAGGAG	4 cut(s)	65, 786, 1785, 2676
BseSI	GKGCMC	1 cut(s)	1115
BseYI	CCCAGC	1 cut(s)	2264
BsgI	GTGCAG	2 cut(s)	1013, 2067
Bsh1236I	CGCG	1 cut(s)	2654
BshNI	GGYRCC	1 cut(s)	2384
BshVI	ATCGAT	1 cut(s)	1383
BsiHKAI	GWGCWC	3 cut(s)	1115, 1350, 1506
BsiHKCI	CYCGRG	3 cut(s)	273, 1649, 2678
BsiSI	CCGG	2 cut(s)	480, 2541
BslFI	GGGAC	4 cut(s)	1216, 1384, 1700, 1763
BslI	CCNNNNNNNGG	6 cut(s)	317, 815, 938, 1212, 1582, 2583
BsmAI	GTCTC	4 cut(s)	781, 835, 1974, 1997
BsmFI	GGGAC	4 cut(s)	1216, 1384, 1700, 1763
BsmI	GAATGC	3 cut(s)	553, 1825, 2403
Bso31I	GGTCTC	1 cut(s)	1997
BsoBI	CYCGRG	3 cut(s)	273, 1649, 2678
Bsp1286I	GDGCHC	3 cut(s)	1115, 1350, 1506
Bsp1407I	TGTACA	1 cut(s)	1531
BspACI	CCGC	2 cut(s)	44, 2100
BspCNI	CTCAG	6 cut(s)	40, 165, 313, 1234, 1311, 2701
BspDI	ATCGAT	1 cut(s)	1383
BspFNI	CGCG	1 cut(s)	2654
BspHI	TCATGA	4 cut(s)	487, 1783, 1834, 2358
BspLI	GGNNCC	9 cut(s)	309, 477, 539, 1098, 1204, 1542, 1574, 2386, 2685
BspMAI	CTGCAG	3 cut(s)	1075, 2098, 2337
BspOI	GCTAGC	1 cut(s)	2081
BspPI	GGATC	4 cut(s)	1388, 1796, 2230, 2730
BspT107I	GGYRCC	1 cut(s)	2384
BspTNI	GGTCTC	1 cut(s)	1997
BsrDI	GCAATG	3 cut(s)	374, 1959, 2109
BsrFI	RCCGGY	1 cut(s)	2540
BsrGI	TGTACA	1 cut(s)	1531
BsrI	ACTGG	6 cut(s)	721, 1519, 1554, 1754, 2265, 2458
BssAI	RCCGGY	1 cut(s)	2540
BssECI	CCNNGG	6 cut(s)	478, 541, 578, 1805, 2667, 2775
BssNAI	GTATAC	2 cut(s)	206, 2277
BssNI	GRCGYC	2 cut(s)	2282, 2573
BssT1I	CCWWGG	3 cut(s)	578, 2667, 2775
Bst1107I	GTATAC	2 cut(s)	206, 2277
Bst2UI	CCWGG	6 cut(s)	118, 140, 384, 542, 1053, 1807
Bst4CI	ACNGT	6 cut(s)	1117, 1375, 2041, 2063, 2237, 2810
Bst6I	CTCTTC	1 cut(s)	225
BstACI	GRCGYC	2 cut(s)	2282, 2573
BstAPI	GCANNNNNTGC	2 cut(s)	1085, 1640
BstAUI	TGTACA	1 cut(s)	1531
BstBAI	YACGTR	1 cut(s)	2441
BstC8I	GCNNGC	8 cut(s)	287, 551, 1398, 1938, 2079, 2432, 2542, 2815
BstDEI	CTNAG	6 cut(s)	48, 152, 300, 1221, 1298, 2688
BstENI	CCTNNNNNAGG	1 cut(s)	1210
BstF5I	GGATG	9 cut(s)	327, 539, 651, 1003, 1309, 2401, 2425, 2588, 2649
BstFNI	CGCG	1 cut(s)	2654
BstHHI	GCGC	1 cut(s)	1243
BstMAI	GTCTC	4 cut(s)	781, 835, 1974, 1997
BstNI	CCWGG	6 cut(s)	118, 140, 384, 542, 1053, 1807
BstNSI	RCATGY	5 cut(s)	116, 1042, 2434, 2665, 2817
BstPAI	GACNNNNGTC	1 cut(s)	2456
BstSCI	CCNGG	7 cut(s)	116, 138, 382, 478, 540, 1051, 1805
BstSFI	CTRYAG	4 cut(s)	684, 1071, 2094, 2333
BstSLI	GKGCMC	1 cut(s)	1115
BstUI	CGCG	1 cut(s)	2654
BstV2I	GAAGAC	3 cut(s)	671, 1035, 2277
BstX2I	RGATCY	1 cut(s)	2606
BstXI	CCANNNNNNTGG	1 cut(s)	585
BstYI	RGATCY	1 cut(s)	2606
BstZ17I	GTATAC	2 cut(s)	206, 2277
Bsu15I	ATCGAT	1 cut(s)	1383
Bsu36I	CCTNAGG	2 cut(s)	48, 1298
BsuI	GTATCC	1 cut(s)	2552
BsuTUI	ATCGAT	1 cut(s)	1383
BtgZI	GCGATG	1 cut(s)	1273
BtsCI	GGATG	9 cut(s)	327, 539, 651, 1003, 1309, 2401, 2425, 2588, 2649
BtsI	GCAGTG	2 cut(s)	1506, 2276
BtsIMutI	CAGTG	4 cut(s)	714, 1506, 1561, 2276
Cac8I	GCNNGC	8 cut(s)	287, 551, 1398, 1938, 2079, 2432, 2542, 2815
CaiI	CAGNNNCTG	1 cut(s)	1085
CciI	TCATGA	4 cut(s)	487, 1783, 1834, 2358
CfoI	GCGC	1 cut(s)	1243
Cfr10I	RCCGGY	1 cut(s)	2540
Cfr13I	GGNCC	6 cut(s)	475, 588, 1031, 1203, 1540, 2107
ClaI	ATCGAT	1 cut(s)	1383
CseI	GACGC	2 cut(s)	2271, 2581
Csp6I	GTAC	7 cut(s)	839, 957, 1186, 1324, 1532, 1622, 1960
CviQI	GTAC	7 cut(s)	839, 957, 1186, 1324, 1532, 1622, 1960
DdeI	CTNAG	6 cut(s)	48, 152, 300, 1221, 1298, 2688
DraI	TTTAAA	2 cut(s)	2160, 2317
DraIII	CACNNNGTG	1 cut(s)	1067
EaeI	YGGCCR	4 cut(s)	41, 411, 547, 2596
Eam1104I	CTCTTC	1 cut(s)	225
EarI	CTCTTC	1 cut(s)	225
Ecl136II	GAGCTC	1 cut(s)	1348
Eco130I	CCWWGG	3 cut(s)	578, 2667, 2775
Eco147I	AGGCCT	1 cut(s)	2129
Eco24I	GRGCYC	1 cut(s)	1350
Eco31I	GGTCTC	1 cut(s)	1997
Eco47I	GGWCC	4 cut(s)	588, 1031, 1203, 1540
Eco53kI	GAGCTC	1 cut(s)	1348
Eco57I	CTGAAG	6 cut(s)	969, 1491, 1862, 2544, 2571, 2587
Eco72I	CACGTG	1 cut(s)	2441
Eco81I	CCTNAGG	2 cut(s)	48, 1298
Eco88I	CYCGRG	3 cut(s)	273, 1649, 2678
EcoICRI	GAGCTC	1 cut(s)	1348
EcoNI	CCTNNNNNAGG	1 cut(s)	1210
EcoO109I	RGGNCCY	1 cut(s)	1203
EcoRI	GAATTC	3 cut(s)	34, 760, 1755
EcoRII	CCWGG	6 cut(s)	116, 138, 382, 540, 1051, 1805
EcoT14I	CCWWGG	3 cut(s)	578, 2667, 2775
EcoT22I	ATGCAT	5 cut(s)	466, 1040, 1636, 1825, 2403
EcoT38I	GRGCYC	1 cut(s)	1350
ErhI	CCWWGG	3 cut(s)	578, 2667, 2775
FaqI	GGGAC	4 cut(s)	1216, 1384, 1700, 1763
FauNDI	CATATG	2 cut(s)	1615, 2464
FbaI	TGATCA	1 cut(s)	2017
FblI	GTMKAC	2 cut(s)	205, 2276
FokI	GGATG	9 cut(s)	334, 546, 638, 1010, 1316, 2388, 2432, 2595, 2656
FriOI	GRGCYC	1 cut(s)	1350
FspBI	CTAG	5 cut(s)	342, 438, 1842, 2078, 2117
GlaI	GCGC	1 cut(s)	1242
GsaI	CCCAGC	1 cut(s)	2268
GsuI	CTGGAG	2 cut(s)	139, 1074
HapII	CCGG	2 cut(s)	480, 2541
HgaI	GACGC	2 cut(s)	2271, 2581
HhaI	GCGC	1 cut(s)	1243
Hin1I	GRCGYC	2 cut(s)	2282, 2573
Hin6I	GCGC	1 cut(s)	1241
HinP1I	GCGC	1 cut(s)	1241
HinfI	GANTC	7 cut(s)	774, 828, 868, 1385, 2423, 2485, 2795
HpaII	CCGG	2 cut(s)	480, 2541
HphI	GGTGA	4 cut(s)	614, 636, 721, 1041
Hpy188I	TCNGA	9 cut(s)	155, 949, 1210, 1711, 2022, 2481, 2551, 2606, 2691
HpyCH4III	ACNGT	6 cut(s)	1117, 1375, 2041, 2063, 2237, 2810
HpyCH4IV	ACGT	3 cut(s)	704, 1620, 2440
HpyF3I	CTNAG	6 cut(s)	48, 152, 300, 1221, 1298, 2688
HpySE526I	ACGT	3 cut(s)	704, 1620, 2440
Hsp92I	GRCGYC	2 cut(s)	2282, 2573
HspAI	GCGC	1 cut(s)	1241
KroI	GCCGGC	1 cut(s)	2540
KroNI	GCCGGC	1 cut(s)	2542
Ksp22I	TGATCA	1 cut(s)	2017
LmnI	GCTCC	2 cut(s)	1772, 2689
LweI	GCATC	7 cut(s)	305, 473, 1228, 1643, 2020, 2410, 2634
MaeI	CTAG	5 cut(s)	342, 438, 1842, 2078, 2117
MaeII	ACGT	3 cut(s)	704, 1620, 2440
MaeIII	GTNAC	6 cut(s)	166, 877, 1745, 2231, 2436, 2458
MfeI	CAATTG	2 cut(s)	765, 1427
MflI	RGATCY	1 cut(s)	2606
MhlI	GDGCHC	3 cut(s)	1115, 1350, 1506
MlsI	TGGCCA	2 cut(s)	413, 549
MluNI	TGGCCA	2 cut(s)	413, 549
MlyI	GAGTC	3 cut(s)	783, 837, 2432
Mox20I	TGGCCA	2 cut(s)	413, 549
Mph1103I	ATGCAT	5 cut(s)	466, 1040, 1636, 1825, 2403
MroNI	GCCGGC	1 cut(s)	2540
MroXI	GAANNNNTTC	1 cut(s)	34
MscI	TGGCCA	2 cut(s)	413, 549
MseI	TTAA	8 cut(s)	131, 1455, 1674, 2159, 2208, 2316, 2367, 2805
MslI	CAYNNNNRTG	4 cut(s)	532, 707, 1629, 2406
Msp20I	TGGCCA	2 cut(s)	413, 549
MspA1I	CMGCKG	1 cut(s)	1496
MspI	CCGG	2 cut(s)	480, 2541
MspR9I	CCNGG	7 cut(s)	118, 140, 384, 480, 542, 1053, 1807
MunI	CAATTG	2 cut(s)	765, 1427
Mva1269I	GAATGC	3 cut(s)	553, 1825, 2403
MvaI	CCWGG	6 cut(s)	118, 140, 384, 542, 1053, 1807
MvnI	CGCG	1 cut(s)	2654
NaeI	GCCGGC	1 cut(s)	2542
NciI	CCSGG	1 cut(s)	480
NdeI	CATATG	2 cut(s)	1615, 2464
NgoMIV	GCCGGC	1 cut(s)	2540
NheI	GCTAGC	1 cut(s)	2077
NlaIV	GGNNCC	9 cut(s)	309, 477, 539, 1098, 1204, 1542, 1574, 2386, 2685
NmuCI	GTSAC	2 cut(s)	2436, 2458
NsiI	ATGCAT	5 cut(s)	466, 1040, 1636, 1825, 2403
NspI	RCATGY	5 cut(s)	116, 1042, 2434, 2665, 2817
OliI	CACNNNNGTG	1 cut(s)	707
PaeI	GCATGC	2 cut(s)	2434, 2817
PaeR7I	CTCGAG	3 cut(s)	273, 1649, 2678
PagI	TCATGA	4 cut(s)	487, 1783, 1834, 2358
PceI	AGGCCT	1 cut(s)	2129
PctI	GAATGC	3 cut(s)	553, 1825, 2403
PdiI	GCCGGC	1 cut(s)	2542
PdmI	GAANNNNTTC	1 cut(s)	34
PfeI	GAWTC	4 cut(s)	868, 1385, 2485, 2795
PflMI	CCANNNNNTGG	1 cut(s)	815
PleI	GAGTC	3 cut(s)	782, 836, 2431
PmaCI	CACGTG	1 cut(s)	2441
PmlI	CACGTG	1 cut(s)	2441
PpsI	GAGTC	3 cut(s)	782, 836, 2431
Ppu21I	YACGTR	1 cut(s)	2441
PpuMI	RGGWCCY	1 cut(s)	1203
PshAI	GACNNNNGTC	1 cut(s)	2456
PsiI	TTATAA	1 cut(s)	1254
Psp124BI	GAGCTC	1 cut(s)	1350
Psp5II	RGGWCCY	1 cut(s)	1203
Psp6I	CCWGG	6 cut(s)	116, 138, 382, 540, 1051, 1805
PspCI	CACGTG	1 cut(s)	2441
PspFI	CCCAGC	1 cut(s)	2264
PspGI	CCWGG	6 cut(s)	116, 138, 382, 540, 1051, 1805
PspN4I	GGNNCC	9 cut(s)	309, 477, 539, 1098, 1204, 1542, 1574, 2386, 2685
PspPI	GGNCC	6 cut(s)	475, 588, 1031, 1203, 1540, 2107
PspPPI	RGGWCCY	1 cut(s)	1203
PstI	CTGCAG	3 cut(s)	1075, 2098, 2337
PstNI	CAGNNNCTG	1 cut(s)	1085
PsuI	RGATCY	1 cut(s)	2606
PvuII	CAGCTG	1 cut(s)	1496
RsaI	GTAC	7 cut(s)	840, 958, 1187, 1325, 1533, 1623, 1961
RsaNI	GTAC	7 cut(s)	839, 957, 1186, 1324, 1532, 1622, 1960
RseI	CAYNNNNRTG	4 cut(s)	532, 707, 1629, 2406
SacI	GAGCTC	1 cut(s)	1350
SaqAI	TTAA	8 cut(s)	131, 1455, 1674, 2159, 2208, 2316, 2367, 2805
Sau96I	GGNCC	6 cut(s)	475, 588, 1031, 1203, 1540, 2107
SchI	GAGTC	3 cut(s)	783, 837, 2432
ScrFI	CCNGG	7 cut(s)	118, 140, 384, 480, 542, 1053, 1807
SduI	GDGCHC	3 cut(s)	1115, 1350, 1506
SfaNI	GCATC	7 cut(s)	305, 473, 1228, 1643, 2020, 2410, 2634
SfcI	CTRYAG	4 cut(s)	684, 1071, 2094, 2333
Sfr274I	CTCGAG	3 cut(s)	273, 1649, 2678
SinI	GGWCC	4 cut(s)	588, 1031, 1203, 1540
SlaI	CTCGAG	3 cut(s)	273, 1649, 2678
SmiMI	CAYNNNNRTG	4 cut(s)	532, 707, 1629, 2406
SmlI	CTYRAG	3 cut(s)	273, 1649, 2678
SmoI	CTYRAG	3 cut(s)	273, 1649, 2678
SpeI	ACTAGT	1 cut(s)	1841
SphI	GCATGC	2 cut(s)	2434, 2817
SseBI	AGGCCT	1 cut(s)	2129
SsiI	CCGC	2 cut(s)	44, 2100
SspI	AATATT	2 cut(s)	1678, 1831
SspMI	CTAG	5 cut(s)	342, 438, 1842, 2078, 2117
SstI	GAGCTC	1 cut(s)	1350
StuI	AGGCCT	1 cut(s)	2129
StyD4I	CCNGG	7 cut(s)	116, 138, 382, 478, 540, 1051, 1805
StyI	CCWWGG	3 cut(s)	578, 2667, 2775
TaaI	ACNGT	6 cut(s)	1117, 1375, 2041, 2063, 2237, 2810
TaiI	ACGT	3 cut(s)	707, 1623, 2443
TaqI	TCGA	6 cut(s)	274, 1383, 1420, 1650, 2322, 2679
TaqII	GACCGA	1 cut(s)	1992
TatI	WGTACW	3 cut(s)	838, 1531, 1959
TauI	GCSGC	2 cut(s)	46, 2102
TfiI	GAWTC	4 cut(s)	868, 1385, 2485, 2795
Tru1I	TTAA	8 cut(s)	131, 1455, 1674, 2159, 2208, 2316, 2367, 2805
Tru9I	TTAA	8 cut(s)	131, 1455, 1674, 2159, 2208, 2316, 2367, 2805
TscAI	CASTG	4 cut(s)	721, 1513, 1561, 2276
TseFI	GTSAC	2 cut(s)	2436, 2458
Tsp45I	GTSAC	2 cut(s)	2436, 2458
TspDTI	ATGAA	9 cut(s)	27, 504, 640, 1035, 1320, 1823, 1832, 2033, 2375
TspGWI	ACGGA	1 cut(s)	258
TspRI	CASTG	4 cut(s)	721, 1513, 1561, 2276
Van91I	CCANNNNNTGG	1 cut(s)	815
VneI	GTGCAC	1 cut(s)	1111
VpaK11BI	GGWCC	4 cut(s)	588, 1031, 1203, 1540
XagI	CCTNNNNNAGG	1 cut(s)	1210
XapI	RAATTY	8 cut(s)	34, 158, 624, 760, 1154, 1193, 1755, 2153
XbaI	TCTAGA	2 cut(s)	341, 437
XceI	RCATGY	5 cut(s)	116, 1042, 2434, 2665, 2817
XcmI	CCANNNNNNNNNTGG	3 cut(s)	114, 2413, 2634
XhoI	CTCGAG	3 cut(s)	273, 1649, 2678
XmiI	GTMKAC	2 cut(s)	205, 2276
XmnI	GAANNNNTTC	1 cut(s)	34
XspI	CTAG	5 cut(s)	342, 438, 1842, 2078, 2117
Zsp2I	ATGCAT	5 cut(s)	466, 1040, 1636, 1825, 2403

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.