Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4D

Orthogroup ID

OG0005094

Physical Location & Seq

Genomic Coordinates Forward (+)

1119616 .. 1130529

Gene Structure 10914 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4DG006300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2826 bp

ATGGTTAGTGGTACTTTGTTCCATTGTAGAAAGAATTCATGGCCCCCTCAGGAGTATATCCCCAGAAACACCTTACAATTGTTTGATTTCGATAGTGCTGCCCCACCAGAACAAGCCTGGAGGAGGAAGTTAAACAGCAATGCTAATCTTCTTAAAGAATTTAGTATTACATTTAGGGAAGCAATAAAGATGGTTAGGTTAGGTGTACGCTTGTGGTCATATGTCAGACAAGAGGCTTCTCATGGAAGGAAAGCACCTATTGATCCTTTCACACGAGGTATTTGCAAGCCATCTGCATCTCAAGGGGTTCCACTCGGAGGGATGGGAAGTGGCAGCATCTCAAGAGGTTTTAGAGGCGAGTTCAGGCAATGGCAAATCATTCCGGGTGTATGTGATGGTTCTCCTGTCATGGCCAATCAATTCTCTATCTTTATATCTAGAGATGGAGGGAGTAAAAATTATGCATCAGTCTTGGCCCCAGGTCAACATGAAGGATTAGGAAAAGCTGGTGATCAGGGTATATCATCATGGGGCTGGAATTTGGGTGGTCAGCATTCCACGTACCATGCTCTATTTCCAAGGGCATGGACTGTATATGATGGCGAACCTGATCCAGAATTGAAAATTTCTTGTCGGCAAATATCGCCATTTATCCCTCATAACTATAGAGATAGTAGTCTTCCTACAGCAGTTTTTGTTTATACGTTGGTGAATACTGGAAAAGAAAGAGCAAAAGTCAGCCTACTATTTACATGGGCGAATTCAATTGGAGGAACGTCACACTTGTCGGGAGACCATGTCAATGAACCATTTATAGGCGAAGATGGAGTCTCTGGTGTACTTCTACATCACAAGACTGCAAAAGGGAGTCCTCCTGTTACTTTTGGAATAGCTGCATGTGAAACACAAAATGTAAGCGTTAGTGTTTTGCCGTCTTTTGGGTTATCAGAAGGAAGTACTGTTACAGCGAAAGAAATGTGGGACAAAATGGTGCAGGATGGACAATTTGATCATGAGAATTTCAATTCGGGGCCATGCATGTCTTCATCACCTGGAGAGACACTTTGTGCTGCAGTTTCAGCTACTGCTTGGGTAGAACCCCATGGGAAGTGCACTGTTGCATTTGGTCTATCGTGGTCATCCCCGAAAGTAAAGTTTTTGAAGGGAAGCTCATACCCAAGGAGGTACACAAAATTCTATGGGACCTCTGAAAGGGCAGCTCAAGACTTGGTGCATGATGCACTAACAAATTATAAGCGATGGGAAGAAGAGATTGAAAATTGGCAAAATCCTATACTCAAGGATGAAAAATTACCAGAATGGTACAAGTTCACGTTGTTCAATGAGCTCTACTTCTTGGTCGCTGGTGGAACAGTCTGGATTGATTCTCCCTCACTAGAAACAAACATCAAGAAGAATCAAAGTCAATTAATAAATGGTGAGTATTTAGATAACAAGGCTTCTGAACCTAAGGTGAATGGCAAACAAGGCGGAGTGGTCGAGGATACTGCAACTGGTTGCCATAGGAGTGCAATATCGGACCTACAAAAACACTATGAAGATGCTGGGAGGTTTCTTTATCTGGAAGGAGTTGAATATATCATGTGGAACACATATGACGTGCACTTCTATGCTTCATTTGCCCTTCTTGAGCTGTTTCCCAAAATTGAACTCAATATACAAAGGGACTTTGCAAAAGCAGTCTTATCTGAAGACGGAAGAAAAGTAAAGTTTCTGGCAGAAGGGAATTGGGGAATTCGTAAGGTTAGAGGAGCTGTCCCCCATGATTTGGGAACACATGATCCTTGGAATGAAATGAATGCGTATAATATTCATGATACTAGTAAGTGGAAGGATCTAAACCCGAAGTTTGTACTTCAGGTGTATAGAGATTTTGCTGCAACAGGGGATATGTCATTTGGAGTTGATGTCTGGCCTGCTGTTCGTGCGGCAATGGTATACATGGAACAATTTGATAGGGACAATGATGGTCTCATTGAGAATGATGGTTTCCCTGACCAGACCTATGACGCTTGGACAGTTCATGGAGTAAGTGCTTACTGTGGTTGCTTGTGGCTTGCTGCACTTCAAGCTGCTGCAACAATGGCCTTTCAACTAGGTGACAAGGCCTTTGCTGAATGGTGCAAAACCAAATATTTGAAGGCCAAACCAGCTTTTGAAGAAAAACTATGGAATGGCTCTTATTTCAATTATGATAGCGGATCAAGTAGTAACAGTAAATCCATACAAGCAGATCAACTGGCCGGACAGTGGTATACTGCATCCTCAGGTTTACCTTCACTTTTTGATGATGTTAAAATCCAAAGTGCTTTGCAGAAAATCTATGATTTTAACGTCATGAAAGTTAAAGGAGGTAGGATGGGTGCTGTAAATGGAATGCACCCTGATGGGAGGGTGGATGAGTCTTGTATGCAGTCTCGTGAAATATGGACTGGCGTCACTTATGGGGTGGCTGCAACGATGATCCTTGCTGGAAAGGAGAAAGAGGCATTTACCACTGCTGAAGGAATTTTTACTGCCGGCTGGTCTGAAGAGGGCTACGGATATGCGTTTCAGACTCCTGAGGGGTGGACGATGGATGGCCACTTCCGATCTCTGATATATATGAGGCCACTGTCTATATGGAGCATGCAGTGGGCATTATCAATGCCCAAGGCAATTCTTGAGGCTCCTAAGGCCAATATAATGGACAGAATCCATATTTCTTCTATTAGTTCACGATCCTCCCACAGCGAAACAGGTGTGAAAAAGATTGCAACCAAGGCAAAGTGCTTCAATAATTCTGTGTTCAATTGTGCATGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0006082 GO:0006629 GO:0007275 GO:0007399 GO:0007417 GO:0008150 GO:0008152 GO:0008202 GO:0008206 GO:0008422 GO:0009056 GO:0009987 GO:0015926 GO:0016020 GO:0016021 GO:0016137 GO:0016139 GO:0016787 GO:0016798 GO:0019752 GO:0021953 GO:0021954 GO:0022008 GO:0030154 GO:0030182 GO:0031224 GO:0032501 GO:0032502 GO:0032787 GO:0043436 GO:0044237 GO:0044238 GO:0044281 GO:0044425 GO:0048468 GO:0048666 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0071704 GO:1901135 GO:1901136 GO:1901360 GO:1901575 GO:1901615 GO:1901657 GO:1901658

KEGG Pathways

Metabolic & Signaling

ko00511 ko00600 ko01100 map00511 map00600 map01100

Pfam Domains

Protein Families

DUF608 Glyco_hydr_116N

Protein Analysis

941

Amino Acids

104.76

Weight (kDa)

6.49

Isoelectric Point (pI)

43.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydr_116N	PF12215	102 - 421	3.4e-106	beta-glucosidase 2, glycosyl-hydrolase family 116 N-term
DUF608	PF04685	523 - 884	1.2e-161	Glycosyl-hydrolase family 116, catalytic region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0005094)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G24180 AT3G24180
fragaria_vesca	FvH4_4g00440 FvH4_4g00440 FvH4_4g00440 FvH4_4g00440 FvH4_4g00440
malus_domestica	MD04G1004800.v1.1
prunus_persica	Prupe.1G004700_v2.0.a1 Prupe.1G004700_v2.0.a1 Prupe.1G004700_v2.0.a1
pyrus_communis	pycom04g00480
rosa_chinensis	RchiOBHm_Chr4g0385861
rosa_laevigata	RLG00000010208
rosa_multiflora	Rmu_co8376727.1_g000001 Rmu_co8521537.1_g000001 Rmu_sc0004773.1_g000004 Rmu_sc0008728.1_g000010
rosa_roxburghii	Rroxscaffold_5G00333460
rosa_rugosa	Rorug01G0169800 Rorug03G0301400 Rorug03G0301500 Rorug03G0301600 Rorug03G0301700 Rorug05G0362900
rosa_samantha	Rh4AG006800 Rh4BG004300 Rh4CG007300 Rh4DG006300
rosa_wichuraiana	Rw4G000370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1254
AccB7I	CCANNNNNTGG	1 cut(s)	1789
AccI	GTMKAC	2 cut(s)	1959, 2276
AciI	CCGC	3 cut(s)	1491, 1949, 2220
AclWI	GGATC	7 cut(s)	257, 605, 1796, 1863, 2230, 2479, 2736
AcoI	YGGCCR	3 cut(s)	411, 2262, 2602
AcsI	RAATTY	9 cut(s)	34, 158, 538, 624, 760, 1018, 1193, 1755, 2529
AcuI	CTGAAG	4 cut(s)	1731, 1862, 2544, 2571
AcyI	GRCGYC	1 cut(s)	2457
AdeI	CACNNNGTG	1 cut(s)	1067
AfaI	GTAC	8 cut(s)	13, 207, 563, 840, 958, 1187, 1325, 1875
AfiI	CCNNNNNNNGG	7 cut(s)	123, 317, 815, 938, 1212, 1789, 2271
AhlI	ACTAGT	1 cut(s)	1841
AjiI	CACGTC	1 cut(s)	1621
AjnI	CCWGG	3 cut(s)	116, 478, 1051
AloI	GAACNNNNNNTCC	2 cut(s)	1786, 1818
Alw21I	GWGCWC	3 cut(s)	1115, 1350, 1626
Alw26I	GTCTC	5 cut(s)	786, 835, 1052, 1997, 2442
Alw44I	GTGCAC	2 cut(s)	1111, 1622
AlwI	GGATC	7 cut(s)	257, 605, 1796, 1863, 2230, 2479, 2736
AlwNI	CAGNNNCTG	1 cut(s)	1085
ApaLI	GTGCAC	2 cut(s)	1111, 1622
ApoI	RAATTY	9 cut(s)	34, 158, 538, 624, 760, 1018, 1193, 1755, 2529
ArsI	GACNNNNNNTTYG	4 cut(s)	813, 845, 2114, 2146
AseI	ATTAAT	1 cut(s)	1430
Asp700I	GAANNNNTTC	1 cut(s)	34
AspS9I	GGNCC	5 cut(s)	42, 475, 1031, 1203, 1540
AsuC2I	CCSGG	1 cut(s)	384
AsuHPI	GGTGA	6 cut(s)	521, 721, 1041, 1451, 1486, 2132
AvaII	GGWCC	2 cut(s)	1203, 1540
AxyI	CCTNAGG	5 cut(s)	48, 1470, 2287, 2583, 2694
BaeGI	GKGCMC	2 cut(s)	1115, 1626
BalI	TGGCCA	2 cut(s)	413, 2604
BanII	GRGCYC	1 cut(s)	1350
BarI	GAAGNNNNNNTAC	2 cut(s)	946, 978
BauI	CACGAG	2 cut(s)	273, 2439
BbsI	GAAGAC	3 cut(s)	671, 1035, 1719
Bbv12I	GWGCWC	3 cut(s)	1115, 1350, 1626
BceAI	ACGGC	1 cut(s)	916
BcgI	CGANNNNNNTGC	6 cut(s)	80, 114, 264, 298, 1490, 1524
BciT130I	CCWGG	3 cut(s)	118, 480, 1053
BciVI	GTATCC	1 cut(s)	1498
BclI	TGATCA	2 cut(s)	511, 1009
BcnI	CCSGG	1 cut(s)	384
BcoDI	GTCTC	5 cut(s)	786, 835, 1052, 1997, 2442
BcuI	ACTAGT	1 cut(s)	1841
BfaI	CTAG	4 cut(s)	438, 1397, 1842, 2117
BfmI	CTRYAG	3 cut(s)	664, 684, 1071
BfuI	GTATCC	1 cut(s)	1498
BmcAI	AGTACT	1 cut(s)	958
Bme1390I	CCNGG	4 cut(s)	118, 384, 480, 1053
Bme18I	GGWCC	2 cut(s)	1203, 1540
BmgBI	CACGTC	1 cut(s)	1621
BmgT120I	GGNCC	5 cut(s)	42, 475, 1031, 1203, 1540
BmiI	GGNNCC	6 cut(s)	44, 309, 477, 1032, 1204, 2691
BmrFI	CCNGG	4 cut(s)	118, 384, 480, 1053
BmsI	GCATC	6 cut(s)	305, 345, 473, 1228, 1552, 2291
BoxI	GACNNNNGTC	1 cut(s)	2456
BpiI	GAAGAC	3 cut(s)	671, 1035, 1719
BpmI	CTGGAG	2 cut(s)	139, 1074
BpuEI	CTTGAG	6 cut(s)	285, 325, 1206, 1283, 1670, 2705
BpuMI	CCSGG	1 cut(s)	384
BsaAI	YACGTR	1 cut(s)	561
BsaHI	GRCGYC	1 cut(s)	2457
BsaI	GGTCTC	2 cut(s)	786, 1997
BsaJI	CCNNGG	7 cut(s)	478, 578, 1102, 1178, 1805, 2673, 2781
BsaXI	ACNNNNNCTCC	4 cut(s)	783, 813, 1914, 1944
Bsc4I	CCNNNNNNNGG	7 cut(s)	123, 317, 815, 938, 1212, 1789, 2271
Bse118I	RCCGGY	1 cut(s)	2540
Bse1I	ACTGG	4 cut(s)	721, 1519, 2265, 2458
Bse21I	CCTNAGG	5 cut(s)	48, 1470, 2287, 2583, 2694
Bse3DI	GCAATG	3 cut(s)	145, 374, 1959
BseBI	CCWGG	3 cut(s)	118, 480, 1053
BseDI	CCNNGG	7 cut(s)	478, 578, 1102, 1178, 1805, 2673, 2781
BseGI	GGATG	8 cut(s)	327, 1003, 1139, 1309, 2282, 2385, 2425, 2605
BseLI	CCNNNNNNNGG	7 cut(s)	123, 317, 815, 938, 1212, 1789, 2271
BseMI	GCAATG	3 cut(s)	145, 374, 1959
BseMII	CTCAG	3 cut(s)	62, 2301, 2574
BseNI	ACTGG	4 cut(s)	721, 1519, 2265, 2458
BseRI	GAGGAG	2 cut(s)	136, 1785
BseSI	GKGCMC	2 cut(s)	1115, 1626
BseYI	CCCAGC	1 cut(s)	1565
BsgI	GTGCAG	2 cut(s)	1013, 2067
BsiHKAI	GWGCWC	3 cut(s)	1115, 1350, 1626
BsiSI	CCGG	3 cut(s)	383, 2265, 2541
BslFI	GGGAC	5 cut(s)	995, 1216, 1700, 1763, 1994
BslI	CCNNNNNNNGG	7 cut(s)	123, 317, 815, 938, 1212, 1789, 2271
BsmAI	GTCTC	5 cut(s)	786, 835, 1052, 1997, 2442
BsmFI	GGGAC	5 cut(s)	995, 1216, 1700, 1763, 1994
BsmI	GAATGC	3 cut(s)	553, 1825, 2403
Bso31I	GGTCTC	2 cut(s)	786, 1997
Bsp1286I	GDGCHC	3 cut(s)	1115, 1350, 1626
Bsp19I	CCATGG	1 cut(s)	1102
BspACI	CCGC	3 cut(s)	1491, 1949, 2220
BspCNI	CTCAG	3 cut(s)	61, 2300, 2575
BspHI	TCATGA	3 cut(s)	1012, 1834, 2358
BspLI	GGNNCC	6 cut(s)	44, 309, 477, 1032, 1204, 2691
BspMAI	CTGCAG	1 cut(s)	1075
BspPI	GGATC	7 cut(s)	257, 605, 1796, 1863, 2230, 2479, 2736
BspTNI	GGTCTC	2 cut(s)	786, 1997
BsrDI	GCAATG	3 cut(s)	145, 374, 1959
BsrFI	RCCGGY	1 cut(s)	2540
BsrI	ACTGG	4 cut(s)	721, 1519, 2265, 2458
BssAI	RCCGGY	1 cut(s)	2540
BssECI	CCNNGG	7 cut(s)	478, 578, 1102, 1178, 1805, 2673, 2781
BssNAI	GTATAC	2 cut(s)	1960, 2277
BssNI	GRCGYC	1 cut(s)	2457
BssSI	CACGAG	2 cut(s)	273, 2439
BssT1I	CCWWGG	6 cut(s)	578, 1102, 1178, 1805, 2673, 2781
Bst1107I	GTATAC	2 cut(s)	1960, 2277
Bst2BI	CACGAG	2 cut(s)	273, 2439
Bst2UI	CCWGG	3 cut(s)	118, 480, 1053
Bst4CI	ACNGT	9 cut(s)	592, 961, 1117, 1375, 2041, 2063, 2237, 2271, 2637
Bst6I	CTCTTC	2 cut(s)	1263, 2547
BstACI	GRCGYC	1 cut(s)	2457
BstBAI	YACGTR	1 cut(s)	561
BstC8I	GCNNGC	6 cut(s)	287, 1938, 2079, 2542, 2651, 2821
BstDEI	CTNAG	5 cut(s)	48, 1470, 2287, 2583, 2694
BstDSI	CCRYGG	1 cut(s)	1102
BstENI	CCTNNNNNAGG	2 cut(s)	121, 1210
BstF5I	GGATG	8 cut(s)	327, 1003, 1139, 1309, 2282, 2385, 2425, 2605
BstMAI	GTCTC	5 cut(s)	786, 835, 1052, 1997, 2442
BstNI	CCWGG	3 cut(s)	118, 480, 1053
BstNSI	RCATGY	4 cut(s)	900, 1042, 2653, 2823
BstPAI	GACNNNNGTC	1 cut(s)	2456
BstSCI	CCNGG	4 cut(s)	116, 382, 478, 1051
BstSFI	CTRYAG	3 cut(s)	664, 684, 1071
BstSLI	GKGCMC	2 cut(s)	1115, 1626
BstV2I	GAAGAC	3 cut(s)	671, 1035, 1719
BstX2I	RGATCY	1 cut(s)	1855
BstXI	CCANNNNNNTGG	2 cut(s)	585, 2707
BstYI	RGATCY	1 cut(s)	1855
BstZ17I	GTATAC	2 cut(s)	1960, 2277
Bsu36I	CCTNAGG	5 cut(s)	48, 1470, 2287, 2583, 2694
BsuI	GTATCC	1 cut(s)	1498
BtgI	CCRYGG	1 cut(s)	1102
BtgZI	GCGATG	1 cut(s)	1273
BtrI	CACGTC	1 cut(s)	1621
BtsCI	GGATG	8 cut(s)	327, 1003, 1139, 1309, 2282, 2385, 2425, 2605
BtsI	GCAGTG	2 cut(s)	2517, 2660
BtsIMutI	CAGTG	5 cut(s)	1113, 2276, 2517, 2633, 2660
Cac8I	GCNNGC	6 cut(s)	287, 1938, 2079, 2542, 2651, 2821
CaiI	CAGNNNCTG	1 cut(s)	1085
CciI	TCATGA	3 cut(s)	1012, 1834, 2358
Cfr10I	RCCGGY	1 cut(s)	2540
Cfr13I	GGNCC	5 cut(s)	42, 475, 1031, 1203, 1540
CseI	GACGC	2 cut(s)	2039, 2446
Csp6I	GTAC	8 cut(s)	12, 206, 562, 839, 957, 1186, 1324, 1874
CviQI	GTAC	8 cut(s)	12, 206, 562, 839, 957, 1186, 1324, 1874
DdeI	CTNAG	5 cut(s)	48, 1470, 2287, 2583, 2694
DraIII	CACNNNGTG	1 cut(s)	1067
EaeI	YGGCCR	3 cut(s)	411, 2262, 2602
Eam1104I	CTCTTC	2 cut(s)	1263, 2547
EarI	CTCTTC	2 cut(s)	1263, 2547
EciI	GGCGGA	1 cut(s)	1506
Ecl136II	GAGCTC	1 cut(s)	1348
Eco130I	CCWWGG	6 cut(s)	578, 1102, 1178, 1805, 2673, 2781
Eco147I	AGGCCT	1 cut(s)	2129
Eco24I	GRGCYC	1 cut(s)	1350
Eco31I	GGTCTC	2 cut(s)	786, 1997
Eco47I	GGWCC	2 cut(s)	1203, 1540
Eco53kI	GAGCTC	1 cut(s)	1348
Eco57I	CTGAAG	4 cut(s)	1731, 1862, 2544, 2571
Eco81I	CCTNAGG	5 cut(s)	48, 1470, 2287, 2583, 2694
EcoICRI	GAGCTC	1 cut(s)	1348
EcoNI	CCTNNNNNAGG	2 cut(s)	121, 1210
EcoO109I	RGGNCCY	1 cut(s)	1203
EcoRI	GAATTC	3 cut(s)	34, 760, 1755
EcoRII	CCWGG	3 cut(s)	116, 478, 1051
EcoT14I	CCWWGG	6 cut(s)	578, 1102, 1178, 1805, 2673, 2781
EcoT22I	ATGCAT	2 cut(s)	466, 1040
EcoT38I	GRGCYC	1 cut(s)	1350
ErhI	CCWWGG	6 cut(s)	578, 1102, 1178, 1805, 2673, 2781
FaqI	GGGAC	5 cut(s)	995, 1216, 1700, 1763, 1994
FauNDI	CATATG	2 cut(s)	220, 1615
FbaI	TGATCA	2 cut(s)	511, 1009
FblI	GTMKAC	2 cut(s)	1959, 2276
FokI	GGATG	8 cut(s)	334, 1010, 1126, 1316, 2269, 2392, 2432, 2612
FriOI	GRGCYC	1 cut(s)	1350
FspBI	CTAG	4 cut(s)	438, 1397, 1842, 2117
GsaI	CCCAGC	1 cut(s)	1569
GsuI	CTGGAG	2 cut(s)	139, 1074
HapII	CCGG	3 cut(s)	383, 2265, 2541
HgaI	GACGC	2 cut(s)	2039, 2446
Hin1I	GRCGYC	1 cut(s)	2457
HincII	GTYRAC	1 cut(s)	485
HindII	GTYRAC	1 cut(s)	485
HinfI	GANTC	7 cut(s)	828, 868, 1385, 1417, 2423, 2578, 2715
HpaII	CCGG	3 cut(s)	383, 2265, 2541
HphI	GGTGA	6 cut(s)	521, 721, 1041, 1451, 1486, 2132
HpyCH4III	ACNGT	9 cut(s)	592, 961, 1117, 1375, 2041, 2063, 2237, 2271, 2637
HpyCH4IV	ACGT	6 cut(s)	560, 704, 776, 1334, 1620, 2355
HpyF3I	CTNAG	5 cut(s)	48, 1470, 2287, 2583, 2694
HpySE526I	ACGT	6 cut(s)	560, 704, 776, 1334, 1620, 2355
Hsp92I	GRCGYC	1 cut(s)	2457
KroI	GCCGGC	1 cut(s)	2540
KroNI	GCCGGC	1 cut(s)	2542
Ksp22I	TGATCA	2 cut(s)	511, 1009
LmnI	GCTCC	3 cut(s)	1772, 2646, 2695
LweI	GCATC	6 cut(s)	305, 345, 473, 1228, 1552, 2291
MaeI	CTAG	4 cut(s)	438, 1397, 1842, 2117
MaeII	ACGT	6 cut(s)	560, 704, 776, 1334, 1620, 2355
MaeIII	GTNAC	6 cut(s)	777, 877, 961, 2120, 2231, 2458
MfeI	CAATTG	3 cut(s)	77, 765, 2812
MflI	RGATCY	1 cut(s)	1855
MhlI	GDGCHC	3 cut(s)	1115, 1350, 1626
MlsI	TGGCCA	2 cut(s)	413, 2604
MluNI	TGGCCA	2 cut(s)	413, 2604
MlyI	GAGTC	4 cut(s)	837, 877, 2432, 2572
Mox20I	TGGCCA	2 cut(s)	413, 2604
Mph1103I	ATGCAT	2 cut(s)	466, 1040
MroNI	GCCGGC	1 cut(s)	2540
MroXI	GAANNNNTTC	1 cut(s)	34
MscI	TGGCCA	2 cut(s)	413, 2604
MseI	TTAA	6 cut(s)	131, 153, 1430, 2316, 2352, 2367
MslI	CAYNNNNRTG	4 cut(s)	801, 1527, 1629, 2406
Msp20I	TGGCCA	2 cut(s)	413, 2604
MspI	CCGG	3 cut(s)	383, 2265, 2541
MspR9I	CCNGG	4 cut(s)	118, 384, 480, 1053
MunI	CAATTG	3 cut(s)	77, 765, 2812
Mva1269I	GAATGC	3 cut(s)	553, 1825, 2403
MvaI	CCWGG	3 cut(s)	118, 480, 1053
NaeI	GCCGGC	1 cut(s)	2542
NciI	CCSGG	1 cut(s)	384
NcoI	CCATGG	1 cut(s)	1102
NdeI	CATATG	2 cut(s)	220, 1615
NgoMIV	GCCGGC	1 cut(s)	2540
NlaIV	GGNNCC	6 cut(s)	44, 309, 477, 1032, 1204, 2691
NmuCI	GTSAC	3 cut(s)	777, 2120, 2458
NsiI	ATGCAT	2 cut(s)	466, 1040
NspI	RCATGY	4 cut(s)	900, 1042, 2653, 2823
PaeI	GCATGC	2 cut(s)	2653, 2823
PagI	TCATGA	3 cut(s)	1012, 1834, 2358
PceI	AGGCCT	1 cut(s)	2129
PctI	GAATGC	3 cut(s)	553, 1825, 2403
PdiI	GCCGGC	1 cut(s)	2542
PdmI	GAANNNNTTC	1 cut(s)	34
PfeI	GAWTC	3 cut(s)	1385, 1417, 2715
PflFI	GACNNNGTC	1 cut(s)	797
PflMI	CCANNNNNTGG	1 cut(s)	1789
PleI	GAGTC	4 cut(s)	836, 876, 2431, 2572
PpsI	GAGTC	4 cut(s)	836, 876, 2431, 2572
Ppu21I	YACGTR	1 cut(s)	561
PpuMI	RGGWCCY	1 cut(s)	1203
PshAI	GACNNNNGTC	1 cut(s)	2456
PshBI	ATTAAT	1 cut(s)	1430
PsiI	TTATAA	1 cut(s)	1254
Psp124BI	GAGCTC	1 cut(s)	1350
Psp5II	RGGWCCY	1 cut(s)	1203
Psp6I	CCWGG	3 cut(s)	116, 478, 1051
PspFI	CCCAGC	1 cut(s)	1565
PspGI	CCWGG	3 cut(s)	116, 478, 1051
PspN4I	GGNNCC	6 cut(s)	44, 309, 477, 1032, 1204, 2691
PspPI	GGNCC	5 cut(s)	42, 475, 1031, 1203, 1540
PspPPI	RGGWCCY	1 cut(s)	1203
PsrI	GAACNNNNNNTAC	2 cut(s)	1662, 1694
PstI	CTGCAG	1 cut(s)	1075
PstNI	CAGNNNCTG	1 cut(s)	1085
PsuI	RGATCY	1 cut(s)	1855
PsyI	GACNNNGTC	1 cut(s)	797
RsaI	GTAC	8 cut(s)	13, 207, 563, 840, 958, 1187, 1325, 1875
RsaNI	GTAC	8 cut(s)	12, 206, 562, 839, 957, 1186, 1324, 1874
RseI	CAYNNNNRTG	4 cut(s)	801, 1527, 1629, 2406
SacI	GAGCTC	1 cut(s)	1350
SaqAI	TTAA	6 cut(s)	131, 153, 1430, 2316, 2352, 2367
Sau96I	GGNCC	5 cut(s)	42, 475, 1031, 1203, 1540
ScaI	AGTACT	1 cut(s)	958
SchI	GAGTC	4 cut(s)	837, 877, 2432, 2572
ScrFI	CCNGG	4 cut(s)	118, 384, 480, 1053
SduI	GDGCHC	3 cut(s)	1115, 1350, 1626
SfaNI	GCATC	6 cut(s)	305, 345, 473, 1228, 1552, 2291
SfcI	CTRYAG	3 cut(s)	664, 684, 1071
SinI	GGWCC	2 cut(s)	1203, 1540
SmiMI	CAYNNNNRTG	4 cut(s)	801, 1527, 1629, 2406
SmlI	CTYRAG	6 cut(s)	300, 340, 1221, 1298, 1649, 2684
SmoI	CTYRAG	6 cut(s)	300, 340, 1221, 1298, 1649, 2684
SpeI	ACTAGT	1 cut(s)	1841
SphI	GCATGC	2 cut(s)	2653, 2823
SseBI	AGGCCT	1 cut(s)	2129
SsiI	CCGC	3 cut(s)	1491, 1949, 2220
SspI	AATATT	2 cut(s)	1831, 2156
SspMI	CTAG	4 cut(s)	438, 1397, 1842, 2117
SstI	GAGCTC	1 cut(s)	1350
StuI	AGGCCT	1 cut(s)	2129
StyD4I	CCNGG	4 cut(s)	116, 382, 478, 1051
StyI	CCWWGG	6 cut(s)	578, 1102, 1178, 1805, 2673, 2781
TaaI	ACNGT	9 cut(s)	592, 961, 1117, 1375, 2041, 2063, 2237, 2271, 2637
TaiI	ACGT	6 cut(s)	563, 707, 779, 1337, 1623, 2358
TaqI	TCGA	2 cut(s)	90, 1500
TatI	WGTACW	3 cut(s)	838, 956, 1873
TauI	GCSGC	1 cut(s)	1952
TfiI	GAWTC	3 cut(s)	1385, 1417, 2715
Tru1I	TTAA	6 cut(s)	131, 153, 1430, 2316, 2352, 2367
Tru9I	TTAA	6 cut(s)	131, 153, 1430, 2316, 2352, 2367
TscAI	CASTG	5 cut(s)	1120, 2276, 2524, 2640, 2660
TseFI	GTSAC	3 cut(s)	777, 2120, 2458
Tsp45I	GTSAC	3 cut(s)	777, 2120, 2458
TspGWI	ACGGA	2 cut(s)	1731, 2577
TspRI	CASTG	5 cut(s)	1120, 2276, 2524, 2640, 2660
Tth111I	GACNNNGTC	1 cut(s)	797
Van91I	CCANNNNNTGG	1 cut(s)	1789
VneI	GTGCAC	2 cut(s)	1111, 1622
VpaK11BI	GGWCC	2 cut(s)	1203, 1540
VspI	ATTAAT	1 cut(s)	1430
XagI	CCTNNNNNAGG	2 cut(s)	121, 1210
XapI	RAATTY	9 cut(s)	34, 158, 538, 624, 760, 1018, 1193, 1755, 2529
XbaI	TCTAGA	1 cut(s)	437
XceI	RCATGY	4 cut(s)	900, 1042, 2653, 2823
XcmI	CCANNNNNNNNNTGG	2 cut(s)	114, 2640
XmiI	GTMKAC	2 cut(s)	1959, 2276
XmnI	GAANNNNTTC	1 cut(s)	34
XspI	CTAG	4 cut(s)	438, 1397, 1842, 2117
ZrmI	AGTACT	1 cut(s)	958
Zsp2I	ATGCAT	2 cut(s)	466, 1040

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.