Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0005094

Physical Location & Seq

Genomic Coordinates Reverse (-)

825771 .. 836312

Gene Structure 10542 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G000370.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2811 bp

ATGGTTAGTGGCACCTTGTTCCATTGTAGAAAGAATTCATGGCCTCCTCAGGAGTATATCCCCAGAAACACCTTACAATTGTTTGATTTTGATAGTGCTGCCCCACCAGAACAAGCCTGGAGGAGGAAGTTAAACAGCAATGCTAATCTTCTTAAAGAATTTAGTATTACATTTAGGGAAGCAATAAAGATGGTTAGGTTAGGTGTACGCTTGTGGTCATATGTCAGACAAGAGGCTTCTCATGGAAGGAAAGCACCTATTGATCCTTTCACACGAGGTATTTGCAAGCCATCTGCATCTCAAGGGGTTCCGCTCGGAGGGATGGGAAGTGGCAGCATCTCAAGAGGTTTTAGAGGCGAGTTCAGGCAATGGCAAATCATTCCGGGTGTATGTGATGGTTCTCCTGTCATGGCCAATCAATTCTCTATCTTTATATCTAGAGATGGAGGGAGTAAAAATTATGCTTCAGTCTTGGCCCCAGGTCAACATGAAGGATTAGGAAAAGCTGGTGATCAGGGTATATCATCGTGGGGCTGGAATTTGGGTGGTCAGCATTCCACGTACCATGCTCTATTTCCAAGGGCATGGACTGTATATGATGGCGAACCTGATCCAGAATTGAAAATTTCTTGTCGGCAAATATCGCCATTTATCCCTCATAACTATAGAGATAGTAGTCTTCCTACAGCAGTTTTTGTTTATACGTTGGTGAATACTGGAAAAGAAAGAGCAAAAGTCAGCCTACTATTTACATGGGCGAATTCAATTGGAGGAACGTCACACTTGTCGGGAGACCATGTCAATGAACCATTTATAGGCGAAGATGGAGTCTCTGGTGTACTTCTACATCACAAGACTGCAAAAGGGAGTCCTCCTGTTACTTTTGGAATAGCTGCATGTGAAACACAAAATGTAAGCGTTAGTGTTTTGCCGTCTTTTGGGTTATCAGAAGGAAGTACTGTTACAGCGAAAGAAATGTGGGACAAAATGGTGCAGGATGGACAATTTGATCATGAGAATTTCAATTCGGGGCCATGCATGTCTTCATCACCTGGAGAGACACTTTGTGCTGCAGTTTCAGCTACTGCTTGGGTAGAACCCCATGGGAAGTGCACTGTTGCATTTGGTCTATCGTGGTCATCCCCGAAAGTAAAGTTTTTGAAGGGAAGCTCATACCCAAGGAGATACACAAAATTCTATGGGACCTCTGAAAGGGCAGCTCAAGACTTGGTGCATGATGCACTAACAAATTATAAGCGATGGGAAGAAGAGATTGAAAATTGGCAAAATCCTATCCTCAAGGATGAAAAATTACCAGAATGGTACAAGTTCACGTTGTTCAATGAGCTCTACTTCTTGGTCGCTGGTGGAACAGTCTGGATTGATTCTCCCTCACTAGAAACAAACATCAAGAAGAATCAAAGTCAATTAATAAATGGTGAGTATTTAGATAACAAGGCTTCTGAACCTAAGGTGAACGGCAAACAAGGCGGAGTGGTCGACGATACTGCAAGTGCAATATCGGACCTACAAAAACACTATGAAGATGCTGGGAGGTTTCTTTATCTGGAAGGAGTTGAATATATCATGTGGAACACATATGACGTGCACTTCTATGCTTCATTTGCCCTTCTTGAGCTGTTTCCCAAAATTGAACTCAATATACAAAGGGACTTTGCAAAAGCAGTCTTGTCTGAAGATGGAAGAAAAGTAAAGTTTCTGGCAGAAGGGAATTGGGGAATTCGTAAGGTTAGAGGAGCTGTCCCCCATGATTTGGGAACACATGATCCTTGGAATGAAATGAATGCGTATAATATTCATGATACTAGTAAGTGGAAGGATCTAAACCCGAAGTTTGTACTTCAGGTGTATAGAGATTTTGATGCAACAGGGGATATGTCATTTGGAGTTGATGTCTGGCCTGCTGTTCGTGCGGCAATGGAATACATGGAACAATTTGATAGGGACAATGATGGTCTCATTGAGAATGATGGTTTCCCTGACCAGACCTATGACGCTTGGACAGTTCATGGAGTAAGTGCTTACTGTGGTTGCTTGTGGCTTGCTGCACTTCAAGCTGCTGCAGCAATGGCCATTCAACTAGGTGACAAGACCTTTGCTGAATGGTGCAAAACCAAATATTTGAAGGCCAAACCAGCTTTTGAAGAAAAACTATGGAATGGCTCTTATTTCAATTATGATAGCGGATCAAGTAGTAATAGTAAATCCATACAAGCAGATCAACTGGCCGGACAGTGGTATACTGCATCCTCAGGTTTACCTTCACTTTTTGATGATGTTAAAATCCAAAGTGCTTTGCAGAAAATCTATGATTTTAACGTCATGAAAGTTAAAGGAGGTAGGATGGGTGCTGTAAATGGAATGCACCCTGATGGGAGGGTGGATGAGTCTTGTATGCAGTCTCGTGAAATATGGACTGGCGTCACTTATGGGGTGGCTGCAACGATGATCCTTGCTGGAAAGGAGAAAGAGGCATTTACCACTGCTGAAGGAATTTTTACTGCCGGCTGGTCTGAAGAGGGCTACGGATATGCGTTTCAGACTCCTGAGGGGTGGACGATGGATGGCCACTTCCGATCTCTGATATATATGAGGCCTCTGTCTATATGGAGCATGCAGCGGGCATTATCAATGCCCAAGGCAATTCTTGAGGCTCCTAAGGCCAATATAATGGACAGAATCCATATTTCTTCTGTTAGTTCACGATCCTCCCACAGCGAAACAGGTGTGAAAAAGATTGCAACCAAGGCAAAGTGCTTCAATAATTCTGTGTTCAATTGTGCATGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0006082 GO:0006629 GO:0007275 GO:0007399 GO:0007417 GO:0008150 GO:0008152 GO:0008202 GO:0008206 GO:0008422 GO:0009056 GO:0009987 GO:0015926 GO:0016020 GO:0016021 GO:0016137 GO:0016139 GO:0016787 GO:0016798 GO:0019752 GO:0021953 GO:0021954 GO:0022008 GO:0030154 GO:0030182 GO:0031224 GO:0032501 GO:0032502 GO:0032787 GO:0043436 GO:0044237 GO:0044238 GO:0044281 GO:0044425 GO:0048468 GO:0048666 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0071704 GO:1901135 GO:1901136 GO:1901360 GO:1901575 GO:1901615 GO:1901657 GO:1901658

KEGG Pathways

Metabolic & Signaling

ko00511 ko00600 ko01100 map00511 map00600 map01100

Pfam Domains

Protein Families

DUF608 Glyco_hydr_116N

Protein Analysis

936

Amino Acids

104.19

Weight (kDa)

6.32

Isoelectric Point (pI)

43.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydr_116N	PF12215	102 - 421	3.4e-106	beta-glucosidase 2, glycosyl-hydrolase family 116 N-term
DUF608	PF04685	518 - 879	3.1e-162	Glycosyl-hydrolase family 116, catalytic region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0005094)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G24180 AT3G24180
fragaria_vesca	FvH4_4g00440 FvH4_4g00440 FvH4_4g00440 FvH4_4g00440 FvH4_4g00440
malus_domestica	MD04G1004800.v1.1
prunus_persica	Prupe.1G004700_v2.0.a1 Prupe.1G004700_v2.0.a1 Prupe.1G004700_v2.0.a1
pyrus_communis	pycom04g00480
rosa_chinensis	RchiOBHm_Chr4g0385861
rosa_laevigata	RLG00000010208
rosa_multiflora	Rmu_co8376727.1_g000001 Rmu_co8521537.1_g000001 Rmu_sc0004773.1_g000004 Rmu_sc0008728.1_g000010
rosa_roxburghii	Rroxscaffold_5G00333460
rosa_rugosa	Rorug01G0169800 Rorug03G0301400 Rorug03G0301500 Rorug03G0301600 Rorug03G0301700 Rorug05G0362900
rosa_samantha	Rh4AG006800 Rh4BG004300 Rh4CG007300 Rh4DG006300
rosa_wichuraiana	Rw4G000370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1254
AccB1I	GGYRCC	1 cut(s)	11
AccB7I	CCANNNNNTGG	1 cut(s)	1774
AccBSI	CCGCTC	1 cut(s)	313
AccI	GTMKAC	2 cut(s)	1500, 2261
AciI	CCGC	5 cut(s)	311, 1491, 1934, 2205, 2641
AclWI	GGATC	7 cut(s)	257, 605, 1781, 1848, 2215, 2464, 2721
AcoI	YGGCCR	4 cut(s)	411, 2091, 2247, 2587
AcsI	RAATTY	9 cut(s)	34, 158, 538, 624, 760, 1018, 1193, 1740, 2514
AcuI	CTGAAG	5 cut(s)	450, 1716, 1847, 2529, 2556
AcyI	GRCGYC	1 cut(s)	2442
AdeI	CACNNNGTG	1 cut(s)	1067
AfaI	GTAC	6 cut(s)	207, 563, 840, 958, 1325, 1860
AfiI	CCNNNNNNNGG	7 cut(s)	123, 317, 815, 938, 1212, 1774, 2256
AhlI	ACTAGT	1 cut(s)	1826
AjiI	CACGTC	1 cut(s)	1606
AjnI	CCWGG	3 cut(s)	116, 478, 1051
AloI	GAACNNNNNNTCC	2 cut(s)	1771, 1803
Alw21I	GWGCWC	3 cut(s)	1115, 1350, 1611
Alw26I	GTCTC	5 cut(s)	786, 835, 1052, 1982, 2427
Alw44I	GTGCAC	2 cut(s)	1111, 1607
AlwI	GGATC	7 cut(s)	257, 605, 1781, 1848, 2215, 2464, 2721
AlwNI	CAGNNNCTG	1 cut(s)	1085
ApaLI	GTGCAC	2 cut(s)	1111, 1607
ApoI	RAATTY	9 cut(s)	34, 158, 538, 624, 760, 1018, 1193, 1740, 2514
ArsI	GACNNNNNNTTYG	4 cut(s)	813, 845, 2099, 2131
AseI	ATTAAT	1 cut(s)	1430
Asp700I	GAANNNNTTC	1 cut(s)	34
AspS9I	GGNCC	4 cut(s)	475, 1031, 1203, 1525
AsuC2I	CCSGG	1 cut(s)	384
AsuHPI	GGTGA	6 cut(s)	521, 721, 1041, 1451, 1486, 2117
AvaII	GGWCC	2 cut(s)	1203, 1525
AxyI	CCTNAGG	5 cut(s)	48, 1470, 2272, 2568, 2679
BaeGI	GKGCMC	2 cut(s)	1115, 1611
BalI	TGGCCA	3 cut(s)	413, 2093, 2589
BanI	GGYRCC	1 cut(s)	11
BanII	GRGCYC	1 cut(s)	1350
BarI	GAAGNNNNNNTAC	2 cut(s)	946, 978
BauI	CACGAG	2 cut(s)	273, 2424
BbsI	GAAGAC	2 cut(s)	671, 1035
Bbv12I	GWGCWC	3 cut(s)	1115, 1350, 1611
BceAI	ACGGC	2 cut(s)	916, 1495
BcgI	CGANNNNNNTGC	4 cut(s)	264, 298, 1490, 1524
BciT130I	CCWGG	3 cut(s)	118, 480, 1053
BclI	TGATCA	2 cut(s)	511, 1009
BcnI	CCSGG	1 cut(s)	384
BcoDI	GTCTC	5 cut(s)	786, 835, 1052, 1982, 2427
BcuI	ACTAGT	1 cut(s)	1826
BfaI	CTAG	4 cut(s)	438, 1397, 1827, 2102
BfmI	CTRYAG	4 cut(s)	664, 684, 1071, 2082
BmcAI	AGTACT	1 cut(s)	958
Bme1390I	CCNGG	4 cut(s)	118, 384, 480, 1053
Bme18I	GGWCC	2 cut(s)	1203, 1525
BmgBI	CACGTC	1 cut(s)	1606
BmgT120I	GGNCC	4 cut(s)	475, 1031, 1203, 1525
BmiI	GGNNCC	6 cut(s)	13, 309, 477, 1032, 1204, 2676
BmrFI	CCNGG	4 cut(s)	118, 384, 480, 1053
BmsI	GCATC	6 cut(s)	305, 345, 1228, 1537, 1873, 2276
BoxI	GACNNNNGTC	1 cut(s)	2441
BpiI	GAAGAC	2 cut(s)	671, 1035
BpmI	CTGGAG	2 cut(s)	139, 1074
BpuEI	CTTGAG	6 cut(s)	285, 325, 1206, 1283, 1655, 2690
BpuMI	CCSGG	1 cut(s)	384
BsaAI	YACGTR	1 cut(s)	561
BsaHI	GRCGYC	1 cut(s)	2442
BsaI	GGTCTC	2 cut(s)	786, 1982
BsaJI	CCNNGG	7 cut(s)	478, 578, 1102, 1178, 1790, 2658, 2766
BsaXI	ACNNNNNCTCC	4 cut(s)	783, 813, 1899, 1929
Bsc4I	CCNNNNNNNGG	7 cut(s)	123, 317, 815, 938, 1212, 1774, 2256
Bse118I	RCCGGY	1 cut(s)	2525
Bse1I	ACTGG	3 cut(s)	721, 2250, 2443
Bse21I	CCTNAGG	5 cut(s)	48, 1470, 2272, 2568, 2679
Bse3DI	GCAATG	4 cut(s)	145, 374, 1944, 2094
BseBI	CCWGG	3 cut(s)	118, 480, 1053
BseDI	CCNNGG	7 cut(s)	478, 578, 1102, 1178, 1790, 2658, 2766
BseGI	GGATG	8 cut(s)	327, 1003, 1139, 1309, 2267, 2370, 2410, 2590
BseLI	CCNNNNNNNGG	7 cut(s)	123, 317, 815, 938, 1212, 1774, 2256
BseMI	GCAATG	4 cut(s)	145, 374, 1944, 2094
BseMII	CTCAG	3 cut(s)	62, 2286, 2559
BseNI	ACTGG	3 cut(s)	721, 2250, 2443
BseRI	GAGGAG	3 cut(s)	36, 136, 1770
BseSI	GKGCMC	2 cut(s)	1115, 1611
BseYI	CCCAGC	1 cut(s)	1550
BsgI	GTGCAG	2 cut(s)	1013, 2052
BshNI	GGYRCC	1 cut(s)	11
BsiHKAI	GWGCWC	3 cut(s)	1115, 1350, 1611
BsiSI	CCGG	3 cut(s)	383, 2250, 2526
BslFI	GGGAC	5 cut(s)	995, 1216, 1685, 1748, 1979
BslI	CCNNNNNNNGG	7 cut(s)	123, 317, 815, 938, 1212, 1774, 2256
BsmAI	GTCTC	5 cut(s)	786, 835, 1052, 1982, 2427
BsmFI	GGGAC	5 cut(s)	995, 1216, 1685, 1748, 1979
BsmI	GAATGC	3 cut(s)	553, 1810, 2388
Bso31I	GGTCTC	2 cut(s)	786, 1982
Bsp1286I	GDGCHC	3 cut(s)	1115, 1350, 1611
Bsp19I	CCATGG	1 cut(s)	1102
BspACI	CCGC	5 cut(s)	311, 1491, 1934, 2205, 2641
BspCNI	CTCAG	3 cut(s)	61, 2285, 2560
BspHI	TCATGA	3 cut(s)	1012, 1819, 2343
BspLI	GGNNCC	6 cut(s)	13, 309, 477, 1032, 1204, 2676
BspMAI	CTGCAG	2 cut(s)	1075, 2086
BspPI	GGATC	7 cut(s)	257, 605, 1781, 1848, 2215, 2464, 2721
BspT107I	GGYRCC	1 cut(s)	11
BspTNI	GGTCTC	2 cut(s)	786, 1982
BsrBI	CCGCTC	1 cut(s)	313
BsrDI	GCAATG	4 cut(s)	145, 374, 1944, 2094
BsrFI	RCCGGY	1 cut(s)	2525
BsrI	ACTGG	3 cut(s)	721, 2250, 2443
BssAI	RCCGGY	1 cut(s)	2525
BssECI	CCNNGG	7 cut(s)	478, 578, 1102, 1178, 1790, 2658, 2766
BssNAI	GTATAC	1 cut(s)	2262
BssNI	GRCGYC	1 cut(s)	2442
BssSI	CACGAG	2 cut(s)	273, 2424
BssT1I	CCWWGG	6 cut(s)	578, 1102, 1178, 1790, 2658, 2766
Bst1107I	GTATAC	1 cut(s)	2262
Bst2BI	CACGAG	2 cut(s)	273, 2424
Bst2UI	CCWGG	3 cut(s)	118, 480, 1053
Bst4CI	ACNGT	7 cut(s)	592, 961, 1117, 1375, 2026, 2048, 2256
Bst6I	CTCTTC	2 cut(s)	1263, 2532
BstACI	GRCGYC	1 cut(s)	2442
BstBAI	YACGTR	1 cut(s)	561
BstC8I	GCNNGC	7 cut(s)	287, 1923, 2064, 2527, 2636, 2643, 2806
BstDEI	CTNAG	5 cut(s)	48, 1470, 2272, 2568, 2679
BstDSI	CCRYGG	1 cut(s)	1102
BstENI	CCTNNNNNAGG	2 cut(s)	121, 1210
BstF5I	GGATG	8 cut(s)	327, 1003, 1139, 1309, 2267, 2370, 2410, 2590
BstMAI	GTCTC	5 cut(s)	786, 835, 1052, 1982, 2427
BstNI	CCWGG	3 cut(s)	118, 480, 1053
BstNSI	RCATGY	4 cut(s)	900, 1042, 2638, 2808
BstPAI	GACNNNNGTC	1 cut(s)	2441
BstSCI	CCNGG	4 cut(s)	116, 382, 478, 1051
BstSFI	CTRYAG	4 cut(s)	664, 684, 1071, 2082
BstSLI	GKGCMC	2 cut(s)	1115, 1611
BstV2I	GAAGAC	2 cut(s)	671, 1035
BstX2I	RGATCY	1 cut(s)	1840
BstXI	CCANNNNNNTGG	2 cut(s)	585, 2692
BstYI	RGATCY	1 cut(s)	1840
BstZ17I	GTATAC	1 cut(s)	2262
Bsu36I	CCTNAGG	5 cut(s)	48, 1470, 2272, 2568, 2679
BtgI	CCRYGG	1 cut(s)	1102
BtgZI	GCGATG	1 cut(s)	1273
BtrI	CACGTC	1 cut(s)	1606
BtsCI	GGATG	8 cut(s)	327, 1003, 1139, 1309, 2267, 2370, 2410, 2590
BtsI	GCAGTG	1 cut(s)	2502
BtsIMutI	CAGTG	3 cut(s)	1113, 2261, 2502
Cac8I	GCNNGC	7 cut(s)	287, 1923, 2064, 2527, 2636, 2643, 2806
CaiI	CAGNNNCTG	1 cut(s)	1085
CciI	TCATGA	3 cut(s)	1012, 1819, 2343
Cfr10I	RCCGGY	1 cut(s)	2525
Cfr13I	GGNCC	4 cut(s)	475, 1031, 1203, 1525
CseI	GACGC	2 cut(s)	2024, 2431
Csp6I	GTAC	6 cut(s)	206, 562, 839, 957, 1324, 1859
CviQI	GTAC	6 cut(s)	206, 562, 839, 957, 1324, 1859
DdeI	CTNAG	5 cut(s)	48, 1470, 2272, 2568, 2679
DraIII	CACNNNGTG	1 cut(s)	1067
EaeI	YGGCCR	4 cut(s)	411, 2091, 2247, 2587
Eam1104I	CTCTTC	2 cut(s)	1263, 2532
EarI	CTCTTC	2 cut(s)	1263, 2532
EciI	GGCGGA	1 cut(s)	1506
Ecl136II	GAGCTC	1 cut(s)	1348
Eco130I	CCWWGG	6 cut(s)	578, 1102, 1178, 1790, 2658, 2766
Eco147I	AGGCCT	1 cut(s)	2617
Eco24I	GRGCYC	1 cut(s)	1350
Eco31I	GGTCTC	2 cut(s)	786, 1982
Eco47I	GGWCC	2 cut(s)	1203, 1525
Eco53kI	GAGCTC	1 cut(s)	1348
Eco57I	CTGAAG	5 cut(s)	450, 1716, 1847, 2529, 2556
Eco81I	CCTNAGG	5 cut(s)	48, 1470, 2272, 2568, 2679
EcoICRI	GAGCTC	1 cut(s)	1348
EcoNI	CCTNNNNNAGG	2 cut(s)	121, 1210
EcoO109I	RGGNCCY	1 cut(s)	1203
EcoRI	GAATTC	3 cut(s)	34, 760, 1740
EcoRII	CCWGG	3 cut(s)	116, 478, 1051
EcoT14I	CCWWGG	6 cut(s)	578, 1102, 1178, 1790, 2658, 2766
EcoT22I	ATGCAT	1 cut(s)	1040
EcoT38I	GRGCYC	1 cut(s)	1350
ErhI	CCWWGG	6 cut(s)	578, 1102, 1178, 1790, 2658, 2766
FaqI	GGGAC	5 cut(s)	995, 1216, 1685, 1748, 1979
FauI	CCCGC	1 cut(s)	2634
FauNDI	CATATG	2 cut(s)	220, 1600
FbaI	TGATCA	2 cut(s)	511, 1009
FblI	GTMKAC	2 cut(s)	1500, 2261
FokI	GGATG	8 cut(s)	334, 1010, 1126, 1316, 2254, 2377, 2417, 2597
FriOI	GRGCYC	1 cut(s)	1350
FspBI	CTAG	4 cut(s)	438, 1397, 1827, 2102
GsaI	CCCAGC	1 cut(s)	1554
GsuI	CTGGAG	2 cut(s)	139, 1074
HapII	CCGG	3 cut(s)	383, 2250, 2526
HgaI	GACGC	2 cut(s)	2024, 2431
Hin1I	GRCGYC	1 cut(s)	2442
HincII	GTYRAC	2 cut(s)	485, 1501
HindII	GTYRAC	2 cut(s)	485, 1501
HinfI	GANTC	7 cut(s)	828, 868, 1385, 1417, 2408, 2563, 2700
HpaII	CCGG	3 cut(s)	383, 2250, 2526
HphI	GGTGA	6 cut(s)	521, 721, 1041, 1451, 1486, 2117
Hpy99I	CGWCG	1 cut(s)	1505
HpyCH4III	ACNGT	7 cut(s)	592, 961, 1117, 1375, 2026, 2048, 2256
HpyCH4IV	ACGT	6 cut(s)	560, 704, 776, 1334, 1605, 2340
HpyF3I	CTNAG	5 cut(s)	48, 1470, 2272, 2568, 2679
HpySE526I	ACGT	6 cut(s)	560, 704, 776, 1334, 1605, 2340
Hsp92I	GRCGYC	1 cut(s)	2442
KroI	GCCGGC	1 cut(s)	2525
KroNI	GCCGGC	1 cut(s)	2527
Ksp22I	TGATCA	2 cut(s)	511, 1009
LmnI	GCTCC	3 cut(s)	1757, 2631, 2680
LweI	GCATC	6 cut(s)	305, 345, 1228, 1537, 1873, 2276
MaeI	CTAG	4 cut(s)	438, 1397, 1827, 2102
MaeII	ACGT	6 cut(s)	560, 704, 776, 1334, 1605, 2340
MaeIII	GTNAC	5 cut(s)	777, 877, 961, 2105, 2443
MbiI	CCGCTC	1 cut(s)	313
MfeI	CAATTG	3 cut(s)	77, 765, 2797
MflI	RGATCY	1 cut(s)	1840
MhlI	GDGCHC	3 cut(s)	1115, 1350, 1611
MlsI	TGGCCA	3 cut(s)	413, 2093, 2589
MluNI	TGGCCA	3 cut(s)	413, 2093, 2589
MlyI	GAGTC	4 cut(s)	837, 877, 2417, 2557
Mox20I	TGGCCA	3 cut(s)	413, 2093, 2589
Mph1103I	ATGCAT	1 cut(s)	1040
MroNI	GCCGGC	1 cut(s)	2525
MroXI	GAANNNNTTC	1 cut(s)	34
MscI	TGGCCA	3 cut(s)	413, 2093, 2589
MseI	TTAA	6 cut(s)	131, 153, 1430, 2301, 2337, 2352
MslI	CAYNNNNRTG	3 cut(s)	801, 1614, 2391
Msp20I	TGGCCA	3 cut(s)	413, 2093, 2589
MspA1I	CMGCKG	1 cut(s)	2641
MspI	CCGG	3 cut(s)	383, 2250, 2526
MspR9I	CCNGG	4 cut(s)	118, 384, 480, 1053
MunI	CAATTG	3 cut(s)	77, 765, 2797
Mva1269I	GAATGC	3 cut(s)	553, 1810, 2388
MvaI	CCWGG	3 cut(s)	118, 480, 1053
NaeI	GCCGGC	1 cut(s)	2527
NciI	CCSGG	1 cut(s)	384
NcoI	CCATGG	1 cut(s)	1102
NdeI	CATATG	2 cut(s)	220, 1600
NgoMIV	GCCGGC	1 cut(s)	2525
NlaIV	GGNNCC	6 cut(s)	13, 309, 477, 1032, 1204, 2676
NmuCI	GTSAC	3 cut(s)	777, 2105, 2443
NsiI	ATGCAT	1 cut(s)	1040
NspI	RCATGY	4 cut(s)	900, 1042, 2638, 2808
PaeI	GCATGC	2 cut(s)	2638, 2808
PagI	TCATGA	3 cut(s)	1012, 1819, 2343
PceI	AGGCCT	1 cut(s)	2617
PctI	GAATGC	3 cut(s)	553, 1810, 2388
PdiI	GCCGGC	1 cut(s)	2527
PdmI	GAANNNNTTC	1 cut(s)	34
PfeI	GAWTC	3 cut(s)	1385, 1417, 2700
PflFI	GACNNNGTC	1 cut(s)	797
PflMI	CCANNNNNTGG	1 cut(s)	1774
PleI	GAGTC	4 cut(s)	836, 876, 2416, 2557
PpsI	GAGTC	4 cut(s)	836, 876, 2416, 2557
Ppu21I	YACGTR	1 cut(s)	561
PpuMI	RGGWCCY	1 cut(s)	1203
PshAI	GACNNNNGTC	1 cut(s)	2441
PshBI	ATTAAT	1 cut(s)	1430
PsiI	TTATAA	1 cut(s)	1254
Psp124BI	GAGCTC	1 cut(s)	1350
Psp5II	RGGWCCY	1 cut(s)	1203
Psp6I	CCWGG	3 cut(s)	116, 478, 1051
PspFI	CCCAGC	1 cut(s)	1550
PspGI	CCWGG	3 cut(s)	116, 478, 1051
PspN4I	GGNNCC	6 cut(s)	13, 309, 477, 1032, 1204, 2676
PspPI	GGNCC	4 cut(s)	475, 1031, 1203, 1525
PspPPI	RGGWCCY	1 cut(s)	1203
PsrI	GAACNNNNNNTAC	2 cut(s)	1647, 1679
PstI	CTGCAG	2 cut(s)	1075, 2086
PstNI	CAGNNNCTG	1 cut(s)	1085
PsuI	RGATCY	1 cut(s)	1840
PsyI	GACNNNGTC	1 cut(s)	797
RsaI	GTAC	6 cut(s)	207, 563, 840, 958, 1325, 1860
RsaNI	GTAC	6 cut(s)	206, 562, 839, 957, 1324, 1859
RseI	CAYNNNNRTG	3 cut(s)	801, 1614, 2391
SacI	GAGCTC	1 cut(s)	1350
SalI	GTCGAC	1 cut(s)	1499
SaqAI	TTAA	6 cut(s)	131, 153, 1430, 2301, 2337, 2352
Sau96I	GGNCC	4 cut(s)	475, 1031, 1203, 1525
ScaI	AGTACT	1 cut(s)	958
SchI	GAGTC	4 cut(s)	837, 877, 2417, 2557
ScrFI	CCNGG	4 cut(s)	118, 384, 480, 1053
SduI	GDGCHC	3 cut(s)	1115, 1350, 1611
SfaNI	GCATC	6 cut(s)	305, 345, 1228, 1537, 1873, 2276
SfcI	CTRYAG	4 cut(s)	664, 684, 1071, 2082
SinI	GGWCC	2 cut(s)	1203, 1525
SmiMI	CAYNNNNRTG	3 cut(s)	801, 1614, 2391
SmlI	CTYRAG	6 cut(s)	300, 340, 1221, 1298, 1634, 2669
SmoI	CTYRAG	6 cut(s)	300, 340, 1221, 1298, 1634, 2669
SpeI	ACTAGT	1 cut(s)	1826
SphI	GCATGC	2 cut(s)	2638, 2808
SseBI	AGGCCT	1 cut(s)	2617
SsiI	CCGC	5 cut(s)	311, 1491, 1934, 2205, 2641
SspI	AATATT	2 cut(s)	1816, 2141
SspMI	CTAG	4 cut(s)	438, 1397, 1827, 2102
SstI	GAGCTC	1 cut(s)	1350
StuI	AGGCCT	1 cut(s)	2617
StyD4I	CCNGG	4 cut(s)	116, 382, 478, 1051
StyI	CCWWGG	6 cut(s)	578, 1102, 1178, 1790, 2658, 2766
TaaI	ACNGT	7 cut(s)	592, 961, 1117, 1375, 2026, 2048, 2256
TaiI	ACGT	6 cut(s)	563, 707, 779, 1337, 1608, 2343
TaqI	TCGA	1 cut(s)	1500
TatI	WGTACW	3 cut(s)	838, 956, 1858
TauI	GCSGC	1 cut(s)	1937
TfiI	GAWTC	3 cut(s)	1385, 1417, 2700
Tru1I	TTAA	6 cut(s)	131, 153, 1430, 2301, 2337, 2352
Tru9I	TTAA	6 cut(s)	131, 153, 1430, 2301, 2337, 2352
TscAI	CASTG	3 cut(s)	1120, 2261, 2509
TseFI	GTSAC	3 cut(s)	777, 2105, 2443
Tsp45I	GTSAC	3 cut(s)	777, 2105, 2443
TspGWI	ACGGA	1 cut(s)	2562
TspRI	CASTG	3 cut(s)	1120, 2261, 2509
Tth111I	GACNNNGTC	1 cut(s)	797
Van91I	CCANNNNNTGG	1 cut(s)	1774
VneI	GTGCAC	2 cut(s)	1111, 1607
VpaK11BI	GGWCC	2 cut(s)	1203, 1525
VspI	ATTAAT	1 cut(s)	1430
XagI	CCTNNNNNAGG	2 cut(s)	121, 1210
XapI	RAATTY	9 cut(s)	34, 158, 538, 624, 760, 1018, 1193, 1740, 2514
XbaI	TCTAGA	1 cut(s)	437
XceI	RCATGY	4 cut(s)	900, 1042, 2638, 2808
XcmI	CCANNNNNNNNNTGG	1 cut(s)	114
XmiI	GTMKAC	2 cut(s)	1500, 2261
XmnI	GAANNNNTTC	1 cut(s)	34
XspI	CTAG	4 cut(s)	438, 1397, 1827, 2102
ZrmI	AGTACT	1 cut(s)	958
Zsp2I	ATGCAT	1 cut(s)	1040

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.