Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr4

Orthogroup ID

OG0005094

Physical Location & Seq

Genomic Coordinates Forward (+)

439135 .. 448319

Gene Structure 9185 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom04g00480.16

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2826 bp

ATGGTTAGTGGGCATTTGTTCCATTGTAGAAAGAATTCATGGCCGCCTGAGGAGTATATCAGCAAAAACACTTTGCACCTGTTTGATTTTGATAGTGCTGCCCCACCAGAACATGCCTGGAGGAGGAAGTTAAACAGCCATGCTAATCTTCTCAGAGAATTTAGTGTTACATTTAGGGAAGCGATAAAGATGGTTCGGTTAGGTATACGCCTGTGGTCATACATCAGGGAAGAGGCTTCTCACGGAAGGAAAGCACCTATTGATCCTTTTACTCGAGAAAGTTGCAAGCCATCTGCAGCTCAGGGGGTTCCACTTGGAGGGATGGGAAGTGGCAGCATATCTAGAGGTTTTAGAGGCGAGTTCAGGCAATGGCAAATCATTCCTGGTATCTGCAAAGGTTCTCCTGTCATGGCCAATCAGTTCTCTATTTTTATATCTAGAGAAGGAACGAATAAAAATTATGCATCAGTCTTGGCCCCGGGTCATCATGAAGGATTAGGAAAAGCTGATGATCAGGGTATATCATCATGGGGATGGAACCTGGGTGGCCAGCATTCAACATACCATGCACTATTTCCAAGGGCATGGACCATTTATGATGGTGAACCTGACCCAGAATTGAAAATTTCTTGTCGGCAAATATCGCCATTCATCCCTCATAATTATAGAGAAAGTAGTCTTCCTACAGCCGTTTTTGTTTACACGTTGGTGAACACTGGAAAAGAAAGAGCCAAAGTCAGCCTTCTATTTACATGGGCGAATTCAATTGGAGGAGTCTCACACTTGTCGGGCGATCATGTGAATGAACCATTTATTGGTGAAGATGGAGTCTCTGGTGTACTTCTACATCACAAGACTGCAAAAGGGAATCCTCCTGTTACATTTGCAATAGCTGCGTGTGAAACTCAAAATGTAAGCGTGAGTGTGTTGCCATGTTTTGGGTTATCTGAAGGAAGTACCACTACGGCGAAAGAAATGTGGAATAAAATGGTGCAGGATGGGCAATTTGGTCGTGATAATTTCAATTCTGGACCATGCATGTCTTCATCACCTGGAGAAACACTCTGTGCTGCAGTAGCAGCTACTGCTTGGGTGGAACCTCATGGGAAGTGCACTGTTGCATTTGGCGTATCATGGTCATCTCCAAAAGTAAAATTTTTGAAGGGAAGCTCATACCATAGGAGGTACACAAAATTCTATGGGACCTCTGAAAGGGCAGCTCAGGACTTGGTGCATGATGCGCTGACAAATTATAAGCGATGGGAAGAAGATATTGAAAAGTGGCAAAGCCCTATCCTCGGGGATGAAACATTACCAGAATGGTACAAGTTCACATTATTCAATGAGCTCTACTTTTTGGTGGCTGGTGGGACAGTTTGGATCGATTCTCCCTTGCCAGCAACAAACATTCCAGACAATCGACACCAATTGACAAATGTTGAAAATACAGATGTTAAGGTTGGTGAAGCTGAAGTAAATAGCAATCACAACGCAGTTGTTGAGCACACTGCTACTGGTTGCCAAAGGAGTGTACATTTGGACCCACAAATTGCCAATGAAGATGTTGGGAGGTTCCTCTATCTGGAAGGAGTGGAATATATCATGTGGAACACATATGACGTACACTTTTATGCATCATTTGCCCTTCTCGAGCTGTTTCCCAAAATTGAACTTAATATTCAAAGGGACTTTGCAAAAGCCGTCTTATCAGAAGATGGAAGAAAAGTAAAGTTTCTTGCGGAGGGTAACTGGGGAATTCGTAAGGTTAGAGGAGCTGTCCCTCATGATCTGGCAACACACGATCCTTGGAACGAAATGAATGCATATAATATTCATGATACTAGTAAGTGGAAGGATTTGAACCCAAAGTTTGTGCTTCAGGTGTATAGAGATTTTTCTGCAACAGGGGATATGTCATTTGGAGTAGATGTTTGGCCTGCTGTTCGTGCTGCAATGGAGTACATGGAACAATTTGACCGGGACAATGATGGTCTCATTGAAAATGATGGTTTCCCTGATCAGACCTACGATGCTTGGACAGTTCATGGAGTAAGTGCTTACTGTGGGTGCTTGTGGCTTGCTGCACTTCAAGCTGCAGCCGCAATGGCCCTTCAACTAGGCGACAAGGCCTTTGCTGAATGGTGCAAAACCAAATTTTTAAAGGCAAAACCATCTTTTGAGGAAAAATTGTGGAATGGTTCTTATTTTAATTACGATAGTGGATCAAGTAGTAACAGTAAGTCTATACAAGCAGATCAACTGGCTGGGCAGTGGTATACGGCATCTTCTGGTTTACCTTCACTTTTTGATGATTTTAAAATCGAAAGCTCTCTGCAGAAAATCTATGATTTCAATGTCATGAAAGTTAAGGGAGGAAAAATGGGTGCCGTAAACGGAATGCATCCCAATGGGAAGGTGGATGAGTCTTGCATGCAGTCACGTGAAATATGGACTGGTGTCACATATGGGGTGGCTGCTCTGATGATTCTTGCTGGAAAGGAGAAAGAGGCATTCGCTACTGCTGAAGGTATTTTTATTGCCGGCTGGTCTGAAGATGGCTTTGGATACGCTTTCCAGACGCCTGAAGGATGGACGATGGACGGCCACTTCAGATCTCTAATATACATGAGGCCACTATCTATTTGGGGGATGCAATGCGCGTTGAACATGCCCAAGGCAATTCTCGAGGCTCCCCAGATCAACATAATGGACAGAATACATTTATCGTCTGTCAGTTCACGATCCTCTCACAACGAAACAGGTGTGAGAAAGATTGCAACCAAGGCAAAGTGCTTTGAAAATTCTGTTTTTAACTGTGCATGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0006082 GO:0006629 GO:0007275 GO:0007399 GO:0007417 GO:0008150 GO:0008152 GO:0008202 GO:0008206 GO:0008422 GO:0009056 GO:0009987 GO:0015926 GO:0016020 GO:0016021 GO:0016137 GO:0016139 GO:0016787 GO:0016798 GO:0019752 GO:0021953 GO:0021954 GO:0022008 GO:0030154 GO:0030182 GO:0031224 GO:0032501 GO:0032502 GO:0032787 GO:0043436 GO:0044237 GO:0044238 GO:0044281 GO:0044425 GO:0048468 GO:0048666 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0071704 GO:1901135 GO:1901136 GO:1901360 GO:1901575 GO:1901615 GO:1901657 GO:1901658

KEGG Pathways

Metabolic & Signaling

ko00511 ko00600 ko01100 map00511 map00600 map01100

Pfam Domains

Protein Families

DUF608 Glyco_hydr_116N

Protein Analysis

942

Amino Acids

104.93

Weight (kDa)

6.19

Isoelectric Point (pI)

42.15

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005094)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G24180 AT3G24180
fragaria_vesca	FvH4_4g00440 FvH4_4g00440 FvH4_4g00440 FvH4_4g00440 FvH4_4g00440
malus_domestica	MD04G1004800.v1.1
prunus_persica	Prupe.1G004700_v2.0.a1 Prupe.1G004700_v2.0.a1 Prupe.1G004700_v2.0.a1
pyrus_communis	pycom04g00480
rosa_chinensis	RchiOBHm_Chr4g0385861
rosa_laevigata	RLG00000010208
rosa_multiflora	Rmu_co8376727.1_g000001 Rmu_co8521537.1_g000001 Rmu_sc0004773.1_g000004 Rmu_sc0008728.1_g000010
rosa_roxburghii	Rroxscaffold_5G00333460
rosa_rugosa	Rorug01G0169800 Rorug03G0301400 Rorug03G0301500 Rorug03G0301600 Rorug03G0301700 Rorug05G0362900
rosa_samantha	Rh4AG006800 Rh4BG004300 Rh4CG007300 Rh4DG006300
rosa_wichuraiana	Rw4G000370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1254
AccB1I	GGYRCC	1 cut(s)	2384
AccB7I	CCANNNNNTGG	2 cut(s)	815, 938
AccI	GTMKAC	2 cut(s)	205, 2276
AccII	CGCG	1 cut(s)	2660
AciI	CCGC	3 cut(s)	44, 1739, 2100
AclWI	GGATC	5 cut(s)	257, 1388, 1796, 2230, 2736
AcoI	YGGCCR	4 cut(s)	41, 411, 547, 2602
AcsI	RAATTY	9 cut(s)	34, 158, 624, 760, 1154, 1193, 1755, 2153, 2800
AcuI	CTGAAG	7 cut(s)	969, 1491, 1862, 2544, 2571, 2593, 2604
AcvI	CACGTG	1 cut(s)	2441
AcyI	GRCGYC	1 cut(s)	2579
AdeI	CACNNNGTG	1 cut(s)	1067
AfaI	GTAC	7 cut(s)	840, 958, 1187, 1325, 1533, 1623, 1961
AfiI	CCNNNNNNNGG	7 cut(s)	123, 317, 815, 938, 1212, 1298, 1582
AflIII	ACRYGT	1 cut(s)	702
AhlI	ACTAGT	1 cut(s)	1841
AjnI	CCWGG	4 cut(s)	116, 382, 540, 1051
AjuI	GAANNNNNNNTTGG	4 cut(s)	798, 830, 2544, 2576
AleI	CACNNNNGTG	1 cut(s)	707
Alw21I	GWGCWC	3 cut(s)	1115, 1350, 1506
Alw26I	GTCTC	3 cut(s)	781, 835, 1997
Alw44I	GTGCAC	1 cut(s)	1111
AlwI	GGATC	5 cut(s)	257, 1388, 1796, 2230, 2736
AlwNI	CAGNNNCTG	1 cut(s)	1085
Ama87I	CYCGRG	5 cut(s)	273, 478, 1298, 1649, 2684
AoxI	GGCC	9 cut(s)	41, 411, 474, 547, 1934, 2106, 2127, 2602, 2630
ApaLI	GTGCAC	1 cut(s)	1111
ApoI	RAATTY	9 cut(s)	34, 158, 624, 760, 1154, 1193, 1755, 2153, 2800
ArsI	GACNNNNNNTTYG	2 cut(s)	2114, 2146
Asp700I	GAANNNNTTC	1 cut(s)	34
AspLEI	GCGC	2 cut(s)	1243, 2660
AspS9I	GGNCC	6 cut(s)	475, 588, 1031, 1203, 1540, 2107
AsuC2I	CCSGG	3 cut(s)	479, 480, 1979
AsuHPI	GGTGA	5 cut(s)	614, 721, 830, 1041, 1475
AvaI	CYCGRG	5 cut(s)	273, 478, 1298, 1649, 2684
AvaII	GGWCC	4 cut(s)	588, 1031, 1203, 1540
AxyI	CCTNAGG	1 cut(s)	48
BaeGI	GKGCMC	1 cut(s)	1115
BalI	TGGCCA	2 cut(s)	413, 549
BanI	GGYRCC	1 cut(s)	2384
BanII	GRGCYC	1 cut(s)	1350
BarI	GAAGNNNNNNTAC	2 cut(s)	946, 978
BbrPI	CACGTG	1 cut(s)	2441
BbsI	GAAGAC	2 cut(s)	671, 1035
Bbv12I	GWGCWC	3 cut(s)	1115, 1350, 1506
BceAI	ACGGC	6 cut(s)	674, 981, 1685, 2295, 2372, 2617
BcgI	CGANNNNNNTGC	6 cut(s)	264, 298, 992, 1026, 1802, 1836
BciT130I	CCWGG	4 cut(s)	118, 384, 542, 1053
BciVI	GTATCC	1 cut(s)	2558
BclI	TGATCA	2 cut(s)	511, 2017
BcnI	CCSGG	3 cut(s)	479, 480, 1979
BcoDI	GTCTC	3 cut(s)	781, 835, 1997
BcuI	ACTAGT	1 cut(s)	1841
BfaI	CTAG	4 cut(s)	342, 438, 1842, 2117
BfmI	CTRYAG	5 cut(s)	294, 684, 1071, 2094, 2333
BfuI	GTATCC	1 cut(s)	2558
BglI	GCCNNNNNGGC	1 cut(s)	2105
BglII	AGATCT	1 cut(s)	2612
Bme1390I	CCNGG	7 cut(s)	118, 384, 479, 480, 542, 1053, 1979
Bme18I	GGWCC	4 cut(s)	588, 1031, 1203, 1540
BmeT110I	CYCGRG	5 cut(s)	273, 478, 1298, 1649, 2684
BmgT120I	GGNCC	6 cut(s)	475, 588, 1031, 1203, 1540, 2107
BmiI	GGNNCC	9 cut(s)	309, 477, 539, 1098, 1204, 1542, 1574, 2386, 2691
BmrFI	CCNGG	7 cut(s)	118, 384, 479, 480, 542, 1053, 1979
BmrI	ACTGGG	1 cut(s)	1759
BmsI	GCATC	7 cut(s)	473, 1228, 1643, 2020, 2291, 2410, 2640
BmuI	ACTGGG	1 cut(s)	1759
BoxI	GACNNNNGTC	1 cut(s)	2456
BpiI	GAAGAC	2 cut(s)	671, 1035
BplI	GAGNNNNNCTC	2 cut(s)	1047, 1079
BpmI	CTGGAG	2 cut(s)	139, 1074
Bpu10I	CCTNAGC	2 cut(s)	300, 1221
BpuMI	CCSGG	3 cut(s)	479, 480, 1979
Bsa29I	ATCGAT	1 cut(s)	1383
BsaAI	YACGTR	1 cut(s)	2441
BsaHI	GRCGYC	1 cut(s)	2579
BsaI	GGTCTC	1 cut(s)	1997
BsaJI	CCNNGG	8 cut(s)	477, 478, 541, 578, 1297, 1805, 2673, 2781
BsaXI	ACNNNNNCTCC	2 cut(s)	1914, 1944
Bsc4I	CCNNNNNNNGG	7 cut(s)	123, 317, 815, 938, 1212, 1298, 1582
Bse118I	RCCGGY	1 cut(s)	2540
Bse1I	ACTGG	5 cut(s)	721, 1519, 1754, 2265, 2458
Bse21I	CCTNAGG	1 cut(s)	48
Bse3DI	GCAATG	4 cut(s)	374, 1959, 2109, 2660
BseBI	CCWGG	4 cut(s)	118, 384, 542, 1053
BseCI	ATCGAT	1 cut(s)	1383
BseDI	CCNNGG	8 cut(s)	477, 478, 541, 578, 1297, 1805, 2673, 2781
BseGI	GGATG	9 cut(s)	327, 539, 651, 1003, 1309, 2401, 2425, 2594, 2655
BseLI	CCNNNNNNNGG	7 cut(s)	123, 317, 815, 938, 1212, 1298, 1582
BseMI	GCAATG	4 cut(s)	374, 1959, 2109, 2660
BseMII	CTCAG	4 cut(s)	39, 166, 314, 1235
BseNI	ACTGG	5 cut(s)	721, 1519, 1754, 2265, 2458
BseRI	GAGGAG	4 cut(s)	65, 136, 786, 1785
BseSI	GKGCMC	1 cut(s)	1115
BseYI	CCCAGC	1 cut(s)	2264
BsgI	GTGCAG	2 cut(s)	1013, 2067
Bsh1236I	CGCG	1 cut(s)	2660
BshFI	GGCC	9 cut(s)	43, 413, 476, 549, 1936, 2108, 2129, 2604, 2632
BshNI	GGYRCC	1 cut(s)	2384
BshVI	ATCGAT	1 cut(s)	1383
BsiHKAI	GWGCWC	3 cut(s)	1115, 1350, 1506
BsiHKCI	CYCGRG	5 cut(s)	273, 478, 1298, 1649, 2684
BsiSI	CCGG	3 cut(s)	479, 1978, 2541
BslFI	GGGAC	5 cut(s)	1216, 1384, 1700, 1763, 1994
BslI	CCNNNNNNNGG	7 cut(s)	123, 317, 815, 938, 1212, 1298, 1582
BsmAI	GTCTC	3 cut(s)	781, 835, 1997
BsmFI	GGGAC	5 cut(s)	1216, 1384, 1700, 1763, 1994
BsmI	GAATGC	4 cut(s)	553, 1825, 2403, 2510
BsnI	GGCC	9 cut(s)	43, 413, 476, 549, 1936, 2108, 2129, 2604, 2632
Bso31I	GGTCTC	1 cut(s)	1997
BsoBI	CYCGRG	5 cut(s)	273, 478, 1298, 1649, 2684
Bsp1286I	GDGCHC	3 cut(s)	1115, 1350, 1506
Bsp1407I	TGTACA	1 cut(s)	1531
BspACI	CCGC	3 cut(s)	44, 1739, 2100
BspANI	GGCC	9 cut(s)	43, 413, 476, 549, 1936, 2108, 2129, 2604, 2632
BspCNI	CTCAG	4 cut(s)	40, 165, 313, 1234
BspDI	ATCGAT	1 cut(s)	1383
BspFNI	CGCG	1 cut(s)	2660
BspHI	TCATGA	4 cut(s)	487, 1783, 1834, 2358
BspLI	GGNNCC	9 cut(s)	309, 477, 539, 1098, 1204, 1542, 1574, 2386, 2691
BspMAI	CTGCAG	4 cut(s)	298, 1075, 2098, 2337
BspPI	GGATC	5 cut(s)	257, 1388, 1796, 2230, 2736
BspT107I	GGYRCC	1 cut(s)	2384
BspTNI	GGTCTC	1 cut(s)	1997
BsrDI	GCAATG	4 cut(s)	374, 1959, 2109, 2660
BsrFI	RCCGGY	1 cut(s)	2540
BsrGI	TGTACA	1 cut(s)	1531
BsrI	ACTGG	5 cut(s)	721, 1519, 1754, 2265, 2458
BssAI	RCCGGY	1 cut(s)	2540
BssECI	CCNNGG	8 cut(s)	477, 478, 541, 578, 1297, 1805, 2673, 2781
BssNAI	GTATAC	2 cut(s)	206, 2277
BssNI	GRCGYC	1 cut(s)	2579
BssT1I	CCWWGG	4 cut(s)	578, 1805, 2673, 2781
Bst1107I	GTATAC	2 cut(s)	206, 2277
Bst2UI	CCWGG	4 cut(s)	118, 384, 542, 1053
Bst4CI	ACNGT	6 cut(s)	1117, 1375, 2041, 2063, 2237, 2816
Bst6I	CTCTTC	1 cut(s)	225
BstACI	GRCGYC	1 cut(s)	2579
BstAPI	GCANNNNNTGC	3 cut(s)	893, 1085, 1640
BstAUI	TGTACA	1 cut(s)	1531
BstBAI	YACGTR	1 cut(s)	2441
BstC8I	GCNNGC	8 cut(s)	287, 551, 1398, 1938, 2079, 2432, 2542, 2821
BstDEI	CTNAG	4 cut(s)	48, 152, 300, 1221
BstENI	CCTNNNNNAGG	2 cut(s)	121, 1210
BstF5I	GGATG	9 cut(s)	327, 539, 651, 1003, 1309, 2401, 2425, 2594, 2655
BstFNI	CGCG	1 cut(s)	2660
BstHHI	GCGC	2 cut(s)	1243, 2660
BstMAI	GTCTC	3 cut(s)	781, 835, 1997
BstNI	CCWGG	4 cut(s)	118, 384, 542, 1053
BstNSI	RCATGY	5 cut(s)	116, 1042, 2434, 2671, 2823
BstPAI	GACNNNNGTC	1 cut(s)	2456
BstSCI	CCNGG	7 cut(s)	116, 382, 477, 478, 540, 1051, 1977
BstSFI	CTRYAG	5 cut(s)	294, 684, 1071, 2094, 2333
BstSLI	GKGCMC	1 cut(s)	1115
BstUI	CGCG	1 cut(s)	2660
BstV2I	GAAGAC	2 cut(s)	671, 1035
BstX2I	RGATCY	1 cut(s)	2612
BstXI	CCANNNNNNTGG	1 cut(s)	585
BstYI	RGATCY	1 cut(s)	2612
BstZ17I	GTATAC	2 cut(s)	206, 2277
Bsu15I	ATCGAT	1 cut(s)	1383
Bsu36I	CCTNAGG	1 cut(s)	48
BsuI	GTATCC	1 cut(s)	2558
BsuRI	GGCC	9 cut(s)	43, 413, 476, 549, 1936, 2108, 2129, 2604, 2632
BsuTUI	ATCGAT	1 cut(s)	1383
BtgZI	GCGATG	1 cut(s)	1273
BtsCI	GGATG	9 cut(s)	327, 539, 651, 1003, 1309, 2401, 2425, 2594, 2655
BtsI	GCAGTG	2 cut(s)	1506, 2276
BtsIMutI	CAGTG	4 cut(s)	714, 1113, 1506, 2276
Cac8I	GCNNGC	8 cut(s)	287, 551, 1398, 1938, 2079, 2432, 2542, 2821
CaiI	CAGNNNCTG	1 cut(s)	1085
CciI	TCATGA	4 cut(s)	487, 1783, 1834, 2358
CfoI	GCGC	2 cut(s)	1243, 2660
Cfr10I	RCCGGY	1 cut(s)	2540
Cfr13I	GGNCC	6 cut(s)	475, 588, 1031, 1203, 1540, 2107
Cfr9I	CCCGGG	1 cut(s)	478
ClaI	ATCGAT	1 cut(s)	1383
CseI	GACGC	1 cut(s)	2587
Csp6I	GTAC	7 cut(s)	839, 957, 1186, 1324, 1532, 1622, 1960
CviQI	GTAC	7 cut(s)	839, 957, 1186, 1324, 1532, 1622, 1960
DdeI	CTNAG	4 cut(s)	48, 152, 300, 1221
DraI	TTTAAA	2 cut(s)	2160, 2317
DraIII	CACNNNGTG	1 cut(s)	1067
EaeI	YGGCCR	4 cut(s)	41, 411, 547, 2602
Eam1104I	CTCTTC	1 cut(s)	225
EarI	CTCTTC	1 cut(s)	225
Ecl136II	GAGCTC	1 cut(s)	1348
Eco130I	CCWWGG	4 cut(s)	578, 1805, 2673, 2781
Eco147I	AGGCCT	1 cut(s)	2129
Eco24I	GRGCYC	1 cut(s)	1350
Eco31I	GGTCTC	1 cut(s)	1997
Eco47I	GGWCC	4 cut(s)	588, 1031, 1203, 1540
Eco53kI	GAGCTC	1 cut(s)	1348
Eco57I	CTGAAG	7 cut(s)	969, 1491, 1862, 2544, 2571, 2593, 2604
Eco72I	CACGTG	1 cut(s)	2441
Eco81I	CCTNAGG	1 cut(s)	48
Eco88I	CYCGRG	5 cut(s)	273, 478, 1298, 1649, 2684
EcoICRI	GAGCTC	1 cut(s)	1348
EcoNI	CCTNNNNNAGG	2 cut(s)	121, 1210
EcoO109I	RGGNCCY	1 cut(s)	1203
EcoRI	GAATTC	3 cut(s)	34, 760, 1755
EcoRII	CCWGG	4 cut(s)	116, 382, 540, 1051
EcoT14I	CCWWGG	4 cut(s)	578, 1805, 2673, 2781
EcoT22I	ATGCAT	5 cut(s)	466, 1040, 1636, 1825, 2403
EcoT38I	GRGCYC	1 cut(s)	1350
ErhI	CCWWGG	4 cut(s)	578, 1805, 2673, 2781
FalI	AAGNNNNNCTT	2 cut(s)	726, 758
FaqI	GGGAC	5 cut(s)	1216, 1384, 1700, 1763, 1994
FauNDI	CATATG	2 cut(s)	1615, 2464
FbaI	TGATCA	2 cut(s)	511, 2017
FblI	GTMKAC	2 cut(s)	205, 2276
FokI	GGATG	9 cut(s)	334, 546, 638, 1010, 1316, 2388, 2432, 2601, 2662
FriOI	GRGCYC	1 cut(s)	1350
FspBI	CTAG	4 cut(s)	342, 438, 1842, 2117
GlaI	GCGC	2 cut(s)	1242, 2659
GsaI	CCCAGC	1 cut(s)	2268
GsuI	CTGGAG	2 cut(s)	139, 1074
HaeIII	GGCC	9 cut(s)	43, 413, 476, 549, 1936, 2108, 2129, 2604, 2632
HapII	CCGG	3 cut(s)	479, 1978, 2541
HgaI	GACGC	1 cut(s)	2587
HhaI	GCGC	2 cut(s)	1243, 2660
Hin1I	GRCGYC	1 cut(s)	2579
Hin6I	GCGC	2 cut(s)	1241, 2658
HinP1I	GCGC	2 cut(s)	1241, 2658
HinfI	GANTC	6 cut(s)	774, 828, 868, 1385, 2423, 2485
HpaII	CCGG	3 cut(s)	479, 1978, 2541
HphI	GGTGA	5 cut(s)	614, 721, 830, 1041, 1475
Hpy188I	TCNGA	8 cut(s)	155, 949, 1210, 1711, 2022, 2481, 2551, 2612
HpyCH4III	ACNGT	6 cut(s)	1117, 1375, 2041, 2063, 2237, 2816
HpyCH4IV	ACGT	3 cut(s)	704, 1620, 2440
HpyF3I	CTNAG	4 cut(s)	48, 152, 300, 1221
HpySE526I	ACGT	3 cut(s)	704, 1620, 2440
Hsp92I	GRCGYC	1 cut(s)	2579
HspAI	GCGC	2 cut(s)	1241, 2658
KroI	GCCGGC	1 cut(s)	2540
KroNI	GCCGGC	1 cut(s)	2542
Ksp22I	TGATCA	2 cut(s)	511, 2017
LmnI	GCTCC	2 cut(s)	1772, 2695
LweI	GCATC	7 cut(s)	473, 1228, 1643, 2020, 2291, 2410, 2640
MaeI	CTAG	4 cut(s)	342, 438, 1842, 2117
MaeII	ACGT	3 cut(s)	704, 1620, 2440
MaeIII	GTNAC	6 cut(s)	166, 877, 1745, 2231, 2436, 2458
MfeI	CAATTG	2 cut(s)	765, 1427
MflI	RGATCY	1 cut(s)	2612
MhlI	GDGCHC	3 cut(s)	1115, 1350, 1506
MlsI	TGGCCA	2 cut(s)	413, 549
MluNI	TGGCCA	2 cut(s)	413, 549
MlyI	GAGTC	3 cut(s)	783, 837, 2432
Mox20I	TGGCCA	2 cut(s)	413, 549
Mph1103I	ATGCAT	5 cut(s)	466, 1040, 1636, 1825, 2403
MroNI	GCCGGC	1 cut(s)	2540
MroXI	GAANNNNTTC	1 cut(s)	34
MscI	TGGCCA	2 cut(s)	413, 549
MseI	TTAA	8 cut(s)	131, 1455, 1674, 2159, 2208, 2316, 2367, 2811
MslI	CAYNNNNRTG	5 cut(s)	532, 707, 801, 1629, 2406
Msp20I	TGGCCA	2 cut(s)	413, 549
MspI	CCGG	3 cut(s)	479, 1978, 2541
MspR9I	CCNGG	7 cut(s)	118, 384, 479, 480, 542, 1053, 1979
MunI	CAATTG	2 cut(s)	765, 1427
Mva1269I	GAATGC	4 cut(s)	553, 1825, 2403, 2510
MvaI	CCWGG	4 cut(s)	118, 384, 542, 1053
MvnI	CGCG	1 cut(s)	2660
NaeI	GCCGGC	1 cut(s)	2542
NciI	CCSGG	3 cut(s)	479, 480, 1979
NdeI	CATATG	2 cut(s)	1615, 2464
NgoMIV	GCCGGC	1 cut(s)	2540
NlaIV	GGNNCC	9 cut(s)	309, 477, 539, 1098, 1204, 1542, 1574, 2386, 2691
NmuCI	GTSAC	2 cut(s)	2436, 2458
NsiI	ATGCAT	5 cut(s)	466, 1040, 1636, 1825, 2403
NspI	RCATGY	5 cut(s)	116, 1042, 2434, 2671, 2823
OliI	CACNNNNGTG	1 cut(s)	707
PaeI	GCATGC	2 cut(s)	2434, 2823
PaeR7I	CTCGAG	3 cut(s)	273, 1649, 2684
PagI	TCATGA	4 cut(s)	487, 1783, 1834, 2358
PceI	AGGCCT	1 cut(s)	2129
PctI	GAATGC	4 cut(s)	553, 1825, 2403, 2510
PdiI	GCCGGC	1 cut(s)	2542
PdmI	GAANNNNTTC	1 cut(s)	34
PfeI	GAWTC	3 cut(s)	868, 1385, 2485
PflMI	CCANNNNNTGG	2 cut(s)	815, 938
PleI	GAGTC	3 cut(s)	782, 836, 2431
PmaCI	CACGTG	1 cut(s)	2441
PmlI	CACGTG	1 cut(s)	2441
PpsI	GAGTC	3 cut(s)	782, 836, 2431
Ppu21I	YACGTR	1 cut(s)	2441
PpuMI	RGGWCCY	1 cut(s)	1203
PshAI	GACNNNNGTC	1 cut(s)	2456
PsiI	TTATAA	1 cut(s)	1254
Psp124BI	GAGCTC	1 cut(s)	1350
Psp5II	RGGWCCY	1 cut(s)	1203
Psp6I	CCWGG	4 cut(s)	116, 382, 540, 1051
PspCI	CACGTG	1 cut(s)	2441
PspFI	CCCAGC	1 cut(s)	2264
PspGI	CCWGG	4 cut(s)	116, 382, 540, 1051
PspN4I	GGNNCC	9 cut(s)	309, 477, 539, 1098, 1204, 1542, 1574, 2386, 2691
PspPI	GGNCC	6 cut(s)	475, 588, 1031, 1203, 1540, 2107
PspPPI	RGGWCCY	1 cut(s)	1203
PstI	CTGCAG	4 cut(s)	298, 1075, 2098, 2337
PstNI	CAGNNNCTG	1 cut(s)	1085
PsuI	RGATCY	1 cut(s)	2612
RsaI	GTAC	7 cut(s)	840, 958, 1187, 1325, 1533, 1623, 1961
RsaNI	GTAC	7 cut(s)	839, 957, 1186, 1324, 1532, 1622, 1960
RseI	CAYNNNNRTG	5 cut(s)	532, 707, 801, 1629, 2406
SacI	GAGCTC	1 cut(s)	1350
SaqAI	TTAA	8 cut(s)	131, 1455, 1674, 2159, 2208, 2316, 2367, 2811
Sau96I	GGNCC	6 cut(s)	475, 588, 1031, 1203, 1540, 2107
SchI	GAGTC	3 cut(s)	783, 837, 2432
ScrFI	CCNGG	7 cut(s)	118, 384, 479, 480, 542, 1053, 1979
SduI	GDGCHC	3 cut(s)	1115, 1350, 1506
SfaNI	GCATC	7 cut(s)	473, 1228, 1643, 2020, 2291, 2410, 2640
SfcI	CTRYAG	5 cut(s)	294, 684, 1071, 2094, 2333
Sfr274I	CTCGAG	3 cut(s)	273, 1649, 2684
SinI	GGWCC	4 cut(s)	588, 1031, 1203, 1540
SlaI	CTCGAG	3 cut(s)	273, 1649, 2684
SmaI	CCCGGG	1 cut(s)	480
SmiMI	CAYNNNNRTG	5 cut(s)	532, 707, 801, 1629, 2406
SmlI	CTYRAG	3 cut(s)	273, 1649, 2684
SmoI	CTYRAG	3 cut(s)	273, 1649, 2684
SpeI	ACTAGT	1 cut(s)	1841
SphI	GCATGC	2 cut(s)	2434, 2823
SseBI	AGGCCT	1 cut(s)	2129
SsiI	CCGC	3 cut(s)	44, 1739, 2100
SspI	AATATT	2 cut(s)	1678, 1831
SspMI	CTAG	4 cut(s)	342, 438, 1842, 2117
SstI	GAGCTC	1 cut(s)	1350
StuI	AGGCCT	1 cut(s)	2129
StyD4I	CCNGG	7 cut(s)	116, 382, 477, 478, 540, 1051, 1977
StyI	CCWWGG	4 cut(s)	578, 1805, 2673, 2781
TaaI	ACNGT	6 cut(s)	1117, 1375, 2041, 2063, 2237, 2816
TaiI	ACGT	3 cut(s)	707, 1623, 2443
TaqI	TCGA	6 cut(s)	274, 1383, 1420, 1650, 2322, 2685
TatI	WGTACW	3 cut(s)	838, 1531, 1959
TauI	GCSGC	2 cut(s)	46, 2102
TfiI	GAWTC	3 cut(s)	868, 1385, 2485
Tru1I	TTAA	8 cut(s)	131, 1455, 1674, 2159, 2208, 2316, 2367, 2811
Tru9I	TTAA	8 cut(s)	131, 1455, 1674, 2159, 2208, 2316, 2367, 2811
TscAI	CASTG	4 cut(s)	721, 1120, 1513, 2276
TseFI	GTSAC	2 cut(s)	2436, 2458
Tsp45I	GTSAC	2 cut(s)	2436, 2458
TspGWI	ACGGA	2 cut(s)	258, 2409
TspMI	CCCGGG	1 cut(s)	478
TspRI	CASTG	4 cut(s)	721, 1120, 1513, 2276
Van91I	CCANNNNNTGG	2 cut(s)	815, 938
VneI	GTGCAC	1 cut(s)	1111
VpaK11BI	GGWCC	4 cut(s)	588, 1031, 1203, 1540
XagI	CCTNNNNNAGG	2 cut(s)	121, 1210
XapI	RAATTY	9 cut(s)	34, 158, 624, 760, 1154, 1193, 1755, 2153, 2800
XbaI	TCTAGA	2 cut(s)	341, 437
XceI	RCATGY	5 cut(s)	116, 1042, 2434, 2671, 2823
XcmI	CCANNNNNNNNNTGG	3 cut(s)	114, 2413, 2640
XhoI	CTCGAG	3 cut(s)	273, 1649, 2684
XmaI	CCCGGG	1 cut(s)	478
XmiI	GTMKAC	2 cut(s)	205, 2276
XmnI	GAANNNNTTC	1 cut(s)	34
XspI	CTAG	4 cut(s)	342, 438, 1842, 2117
Zsp2I	ATGCAT	5 cut(s)	466, 1040, 1636, 1825, 2403

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.