FvH4_4g04100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0010791
Physical Location & Seq
Reverse (-)
3554145 .. 3554570
426 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g04100.t1

Sequence Viewer

Length: 426 bp
ATGAGTTACCAGGTCCATAACAGCCGTGGGATCCCGCCACGTGGCGCGCCCAGAAAGCGGAAGGAGAGAGACGATGGCTCCGAGCCGCTCGGCTTTATGCGACCACCATCGGCTCCGACTGCTGCTAAGCCTCCAACGACACCGCCGGCTGTGTCGTCGAATCGCCTCCTGGCCGGCTACATGGCGTACGAGTTTATAACCAGGGGAACGCTTCTGGGCCAGGAGTGCTGCTCGGCTGTTCCGGCCGAGCTGAGGAGGGTGGTGAAGCCGGTCGGAGCCGAGCCGGTTAAGGAGAGAGAGACGGGATCGGGTGAGGCTGCGGTGCAGGATAGGTACGCTGAGGTGGCGAGCATACTGAAGACGGAAGGGGCCCACATTCCGGGGATTGTGAACCCGACGCAACTGGCTCGGTGGATTCAGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

142

Amino Acids

15.25

Weight (kDa)

9.51

Isoelectric Point (pI)

52.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010791)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23440
fragaria_vesca FvH4_4g04100
malus_domestica MD13G1217500.v1.1 MD16G1222600.v1.1
prunus_persica Prupe.1G044300_v2.0.a1 Prupe.1G044300_v2.0.a1
pyrus_communis pycom13g19090 pycom16g18650
rosa_chinensis RchiOBHm_Chr4g0394481
rosa_laevigata RLG00000009695
rosa_multiflora Rmu_sc0000200.1_g000024 Rmu_sc0013311.1_g000001
rosa_roxburghii Rroxscaffold_5G00339510
rosa_rugosa Rorug03G0365800.1
rosa_samantha Rh4AG052300 Rh4BG050200 Rh4CG056600 Rh4DG049300
rosa_wichuraiana Rw0G003380 Rw4G004220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 197
AccBSI CCGCTC 1 cut(s) 88
AccII CGCG 1 cut(s) 47
AciI CCGC 5 cut(s) 35, 58, 86, 143, 320
AclWI GGATC 3 cut(s) 25, 38, 313
AcoI YGGCCR 2 cut(s) 171, 243
AcuI CTGAAG 1 cut(s) 377
AcvI CACGTG 1 cut(s) 41
AfaI GTAC 2 cut(s) 188, 335
AfiI CCNNNNNNNGG 4 cut(s) 41, 57, 252, 379
AjnI CCWGG 4 cut(s) 9, 168, 200, 219
AluBI AGCT 1 cut(s) 250
AluI AGCT 1 cut(s) 250
Alw26I GTCTC 2 cut(s) 63, 293
AlwI GGATC 3 cut(s) 25, 38, 313
AoxI GGCC 4 cut(s) 171, 217, 243, 369
ApaI GGGCCC 1 cut(s) 373
ApeKI GCWGC 3 cut(s) 122, 228, 317
AscI GGCGCGCC 1 cut(s) 45
AspLEI GCGC 2 cut(s) 47, 49
AspS9I GGNCC 4 cut(s) 13, 217, 369, 370
AsuC2I CCSGG 1 cut(s) 381
AsuHPI GGTGA 2 cut(s) 274, 323
AvaII GGWCC 1 cut(s) 13
BaeGI GKGCMC 1 cut(s) 373
BamHI GGATCC 1 cut(s) 30
BanII GRGCYC 1 cut(s) 373
BbrPI CACGTG 1 cut(s) 41
BbsI GAAGAC 1 cut(s) 365
BbvCI CCTCAGC 2 cut(s) 251, 339
BbvI GCAGC 3 cut(s) 109, 215, 304
BccI CCATC 2 cut(s) 68, 115
BceAI ACGGC 1 cut(s) 9
BcgI CGANNNNNNTGC 2 cut(s) 389, 423
BciT130I CCWGG 4 cut(s) 11, 170, 202, 221
BcnI CCSGG 1 cut(s) 381
BcoDI GTCTC 2 cut(s) 63, 293
BisI GCNGC 4 cut(s) 86, 123, 229, 318
BlpI GCTNAGC 1 cut(s) 126
BlsI GCNGC 4 cut(s) 87, 124, 230, 319
Bme1390I CCNGG 5 cut(s) 11, 170, 202, 221, 381
Bme18I GGWCC 1 cut(s) 13
BmgT120I GGNCC 4 cut(s) 13, 217, 369, 370
BmiI GGNNCC 6 cut(s) 32, 79, 114, 277, 370, 371
BmrFI CCNGG 5 cut(s) 11, 170, 202, 221, 381
BpiI GAAGAC 1 cut(s) 365
BplI GAGNNNNNCTC 2 cut(s) 215, 247
Bpu10I CCTNAGC 2 cut(s) 251, 339
Bpu1102I GCTNAGC 1 cut(s) 126
BpuMI CCSGG 1 cut(s) 381
BsaAI YACGTR 1 cut(s) 41
BsaJI CCNNGG 3 cut(s) 25, 201, 380
BsaXI ACNNNNNCTCC 2 cut(s) 62, 92
Bsc4I CCNNNNNNNGG 4 cut(s) 41, 57, 252, 379
Bse118I RCCGGY 4 cut(s) 145, 173, 268, 283
Bse1I ACTGG 1 cut(s) 408
BseBI CCWGG 4 cut(s) 11, 170, 202, 221
BseDI CCNNGG 3 cut(s) 25, 201, 380
BseLI CCNNNNNNNGG 4 cut(s) 41, 57, 252, 379
BseMII CTCAG 2 cut(s) 242, 330
BseNI ACTGG 1 cut(s) 408
BsePI GCGCGC 1 cut(s) 45
BseRI GAGGAG 1 cut(s) 268
BseSI GKGCMC 1 cut(s) 373
BseX3I CGGCCG 1 cut(s) 243
BseXI GCAGC 3 cut(s) 109, 215, 304
BsgI GTGCAG 1 cut(s) 344
Bsh1236I CGCG 1 cut(s) 47
Bsh1285I CGRYCG 2 cut(s) 246, 273
BshFI GGCC 4 cut(s) 173, 219, 245, 371
BsiEI CGRYCG 2 cut(s) 246, 273
BsiSI CCGG 6 cut(s) 146, 174, 242, 269, 284, 380
BsiWI CGTACG 1 cut(s) 186
BslI CCNNNNNNNGG 4 cut(s) 41, 57, 252, 379
BsmAI GTCTC 2 cut(s) 63, 293
BsmBI CGTCTC 2 cut(s) 63, 293
BsnI GGCC 4 cut(s) 173, 219, 245, 371
Bsp120I GGGCCC 1 cut(s) 369
Bsp1286I GDGCHC 1 cut(s) 373
Bsp143I GATC 2 cut(s) 30, 305
Bsp1720I GCTNAGC 1 cut(s) 126
BspACI CCGC 5 cut(s) 35, 58, 86, 143, 320
BspANI GGCC 4 cut(s) 173, 219, 245, 371
BspCNI CTCAG 2 cut(s) 243, 331
BspFNI CGCG 1 cut(s) 47
BspLI GGNNCC 6 cut(s) 32, 79, 114, 277, 370, 371
BspPI GGATC 3 cut(s) 25, 38, 313
BsrBI CCGCTC 1 cut(s) 88
BsrFI RCCGGY 4 cut(s) 145, 173, 268, 283
BsrI ACTGG 1 cut(s) 408
BssAI RCCGGY 4 cut(s) 145, 173, 268, 283
BssECI CCNNGG 3 cut(s) 25, 201, 380
BssHII GCGCGC 1 cut(s) 45
BssMI GATC 2 cut(s) 30, 305
Bst2UI CCWGG 4 cut(s) 11, 170, 202, 221
BstBAI YACGTR 1 cut(s) 41
BstC8I GCNNGC 4 cut(s) 47, 147, 175, 349
BstDEI CTNAG 3 cut(s) 126, 251, 339
BstDSI CCRYGG 1 cut(s) 25
BstFNI CGCG 1 cut(s) 47
BstHHI GCGC 2 cut(s) 47, 49
BstKTI GATC 2 cut(s) 33, 308
BstMAI GTCTC 2 cut(s) 63, 293
BstMBI GATC 2 cut(s) 30, 305
BstMCI CGRYCG 2 cut(s) 246, 273
BstMWI GCNNNNNNNGC 5 cut(s) 55, 119, 225, 242, 344
BstNI CCWGG 4 cut(s) 11, 170, 202, 221
BstSCI CCNGG 5 cut(s) 9, 168, 200, 219, 379
BstSLI GKGCMC 1 cut(s) 373
BstUI CGCG 1 cut(s) 47
BstV1I GCAGC 3 cut(s) 109, 215, 304
BstV2I GAAGAC 1 cut(s) 365
BstX2I RGATCY 1 cut(s) 30
BstYI RGATCY 1 cut(s) 30
BstZI CGGCCG 1 cut(s) 243
BsuRI GGCC 4 cut(s) 173, 219, 245, 371
BtgI CCRYGG 1 cut(s) 25
Cac8I GCNNGC 4 cut(s) 47, 147, 175, 349
CfoI GCGC 2 cut(s) 47, 49
Cfr10I RCCGGY 4 cut(s) 145, 173, 268, 283
Cfr13I GGNCC 4 cut(s) 13, 217, 369, 370
CseI GACGC 1 cut(s) 406
CsiI ACCWGGT 1 cut(s) 9
Csp6I GTAC 2 cut(s) 187, 334
CviAII CATG 1 cut(s) 181
CviQI GTAC 2 cut(s) 187, 334
DdeI CTNAG 3 cut(s) 126, 251, 339
DpnI GATC 2 cut(s) 32, 307
DpnII GATC 2 cut(s) 30, 305
EaeI YGGCCR 2 cut(s) 171, 243
EagI CGGCCG 1 cut(s) 243
EclXI CGGCCG 1 cut(s) 243
Eco24I GRGCYC 1 cut(s) 373
Eco47I GGWCC 1 cut(s) 13
Eco52I CGGCCG 1 cut(s) 243
Eco57I CTGAAG 1 cut(s) 377
Eco72I CACGTG 1 cut(s) 41
EcoO109I RGGNCCY 1 cut(s) 369
EcoRII CCWGG 4 cut(s) 9, 168, 200, 219
EcoT38I GRGCYC 1 cut(s) 373
Esp3I CGTCTC 2 cut(s) 63, 293
FaeI CATG 1 cut(s) 184
FaiI YATR 5 cut(s) 18, 98, 182, 197, 353
FatI CATG 1 cut(s) 180
FauI CCCGC 1 cut(s) 42
Fnu4HI GCNGC 4 cut(s) 86, 123, 229, 318
FriOI GRGCYC 1 cut(s) 373
Fsp4HI GCNGC 4 cut(s) 86, 123, 229, 318
GlaI GCGC 2 cut(s) 46, 48
GluI GCNGC 4 cut(s) 86, 123, 229, 318
HaeIII GGCC 4 cut(s) 173, 219, 245, 371
HapII CCGG 6 cut(s) 146, 174, 242, 269, 284, 380
HgaI GACGC 1 cut(s) 406
HhaI GCGC 2 cut(s) 47, 49
Hin1II CATG 1 cut(s) 184
Hin6I GCGC 2 cut(s) 45, 47
HinP1I GCGC 2 cut(s) 45, 47
HinfI GANTC 2 cut(s) 160, 415
HpaII CCGG 6 cut(s) 146, 174, 242, 269, 284, 380
HphI GGTGA 2 cut(s) 274, 323
Hpy166II GTNNAC 1 cut(s) 391
Hpy188I TCNGA 4 cut(s) 82, 117, 275, 420
Hpy8I GTNNAC 1 cut(s) 391
Hpy99I CGWCG 2 cut(s) 160, 400
HpyAV CCTTC 2 cut(s) 55, 359
HpyCH4IV ACGT 1 cut(s) 40
HpyCH4V TGCA 1 cut(s) 325
HpyF10VI GCNNNNNNNGC 5 cut(s) 55, 119, 225, 242, 344
HpyF3I CTNAG 3 cut(s) 126, 251, 339
HpySE526I ACGT 1 cut(s) 40
Hsp92II CATG 1 cut(s) 184
HspAI GCGC 2 cut(s) 45, 47
KroI GCCGGC 2 cut(s) 145, 173
KroNI GCCGGC 2 cut(s) 147, 175
Kzo9I GATC 2 cut(s) 30, 305
LmnI GCTCC 3 cut(s) 83, 118, 275
Lsp1109I GCAGC 3 cut(s) 109, 215, 304
MabI ACCWGGT 1 cut(s) 9
MaeII ACGT 1 cut(s) 40
MaeIII GTNAC 1 cut(s) 5
MalI GATC 2 cut(s) 32, 307
MbiI CCGCTC 1 cut(s) 88
MboI GATC 2 cut(s) 30, 305
MboII GAAGA 1 cut(s) 370
MflI RGATCY 1 cut(s) 30
MhlI GDGCHC 1 cut(s) 373
MmeI TCCRAC 3 cut(s) 140, 158, 253
MnlI CCTC 6 cut(s) 141, 176, 246, 249, 307, 334
MroNI GCCGGC 2 cut(s) 145, 173
MseI TTAA 1 cut(s) 288
MspI CCGG 6 cut(s) 146, 174, 242, 269, 284, 380
MspR9I CCNGG 5 cut(s) 11, 170, 202, 221, 381
MvaI CCWGG 4 cut(s) 11, 170, 202, 221
MvnI CGCG 1 cut(s) 47
MwoI GCNNNNNNNGC 5 cut(s) 55, 119, 225, 242, 344
NaeI GCCGGC 2 cut(s) 147, 175
NciI CCSGG 1 cut(s) 381
NdeII GATC 2 cut(s) 30, 305
NgoMIV GCCGGC 2 cut(s) 145, 173
NlaIII CATG 1 cut(s) 184
NlaIV GGNNCC 6 cut(s) 32, 79, 114, 277, 370, 371
NmeAIII GCCGAG 4 cut(s) 69, 212, 271, 304
PalAI GGCGCGCC 1 cut(s) 45
PauI GCGCGC 1 cut(s) 45
PcsI WCGNNNNNNNCGW 1 cut(s) 78
PdiI GCCGGC 2 cut(s) 147, 175
PfeI GAWTC 2 cut(s) 160, 415
Pfl23II CGTACG 1 cut(s) 186
PkrI GCNGC 4 cut(s) 87, 124, 230, 319
PmaCI CACGTG 1 cut(s) 41
PmlI CACGTG 1 cut(s) 41
Ppu21I YACGTR 1 cut(s) 41
PsiI TTATAA 1 cut(s) 197
Psp6I CCWGG 4 cut(s) 9, 168, 200, 219
PspCI CACGTG 1 cut(s) 41
PspGI CCWGG 4 cut(s) 9, 168, 200, 219
PspLI CGTACG 1 cut(s) 186
PspN4I GGNNCC 6 cut(s) 32, 79, 114, 277, 370, 371
PspOMI GGGCCC 1 cut(s) 369
PspPI GGNCC 4 cut(s) 13, 217, 369, 370
PsuI RGATCY 1 cut(s) 30
PteI GCGCGC 1 cut(s) 45
RsaI GTAC 2 cut(s) 188, 335
RsaNI GTAC 2 cut(s) 187, 334
SaqAI TTAA 1 cut(s) 288
SatI GCNGC 4 cut(s) 86, 123, 229, 318
Sau3AI GATC 2 cut(s) 30, 305
Sau96I GGNCC 4 cut(s) 13, 217, 369, 370
ScrFI CCNGG 5 cut(s) 11, 170, 202, 221, 381
SduI GDGCHC 1 cut(s) 373
SetI ASST 5 cut(s) 15, 43, 252, 335, 345
SexAI ACCWGGT 1 cut(s) 9
SgsI GGCGCGCC 1 cut(s) 45
SinI GGWCC 1 cut(s) 13
SsiI CCGC 5 cut(s) 35, 58, 86, 143, 320
StyD4I CCNGG 5 cut(s) 9, 168, 200, 219, 379
TaiI ACGT 1 cut(s) 43
TaqI TCGA 1 cut(s) 158
TauI GCSGC 1 cut(s) 88
TfiI GAWTC 2 cut(s) 160, 415
Tru1I TTAA 1 cut(s) 288
Tru9I TTAA 1 cut(s) 288
TseI GCWGC 3 cut(s) 122, 228, 317
TspGWI ACGGA 1 cut(s) 377
VpaK11BI GGWCC 1 cut(s) 13
XcmI CCANNNNNNNNNTGG 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.