MD16G1222600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0010791
Physical Location & Seq
Reverse (-)
22376874 .. 22377338
465 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1222600.v1.1.491

Sequence Viewer

Length: 465 bp
ATGAATTTCCAGCAAAACCACAGCCGTAGGATTCTGCCACCTGTCCAGCCCAGAAAGCGAAAGGAGAGCTCATCGAACGGCTTTAAACAACCGGCTCCGAAGGCTCCAAAGGCTCCAAGCACAACGACGTTTCCAAGGGCTCGGAGCAAATCTTCGGCTCCAAAGGCTTCTAGCAAAACGACATCTTCGGCGCCGTCGTCCAATCGACTTCTGGCCGGCTACATGGCGTACGAGTTTTTAACCAAGGGCACACTGTTTGGTCAGAAGTTCGACCCGGCTGGAGATGAGGCAGTTCCGCTGGCGGGGAAGCCGATCGAATCCAGACAAGGAGCTGGTACGGGCGCAGCGCAGGAGAGTTACTCTGAGGTGGCGAGCTTACTGAAGACGGATGGGGCCCACATTCCGGGGATTGTAAACCCGACGCAACTGGCGAAATGGATTCAGATGGGACAGTGCTTGTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

155

Amino Acids

16.51

Weight (kDa)

10.21

Isoelectric Point (pI)

60.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010791)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23440
fragaria_vesca FvH4_4g04100
malus_domestica MD13G1217500.v1.1 MD16G1222600.v1.1
prunus_persica Prupe.1G044300_v2.0.a1 Prupe.1G044300_v2.0.a1
pyrus_communis pycom13g19090 pycom16g18650
rosa_chinensis RchiOBHm_Chr4g0394481
rosa_laevigata RLG00000009695
rosa_multiflora Rmu_sc0000200.1_g000024 Rmu_sc0013311.1_g000001
rosa_roxburghii Rroxscaffold_5G00339510
rosa_rugosa Rorug03G0365800.1
rosa_samantha Rh4AG052300 Rh4BG050200 Rh4CG056600 Rh4DG049300
rosa_wichuraiana Rw0G003380 Rw4G004220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 190
AciI CCGC 2 cut(s) 296, 302
AcoI YGGCCR 1 cut(s) 213
AcsI RAATTY 1 cut(s) 4
AcuI CTGAAG 1 cut(s) 401
AcyI GRCGYC 1 cut(s) 191
AfaI GTAC 2 cut(s) 230, 337
AfiI CCNNNNNNNGG 2 cut(s) 302, 403
AluBI AGCT 3 cut(s) 69, 332, 375
AluI AGCT 3 cut(s) 69, 332, 375
Alw21I GWGCWC 1 cut(s) 71
AoxI GGCC 2 cut(s) 213, 393
ApaI GGGCCC 1 cut(s) 397
ApeKI GCWGC 1 cut(s) 344
ApoI RAATTY 1 cut(s) 4
AspLEI GCGC 3 cut(s) 193, 344, 349
AspS9I GGNCC 2 cut(s) 393, 394
AsuC2I CCSGG 2 cut(s) 275, 405
BaeGI GKGCMC 2 cut(s) 251, 397
BanI GGYRCC 1 cut(s) 190
BanII GRGCYC 3 cut(s) 71, 142, 397
BbsI GAAGAC 1 cut(s) 389
Bbv12I GWGCWC 1 cut(s) 71
BbvI GCAGC 1 cut(s) 356
BccI CCATC 2 cut(s) 383, 439
BceAI ACGGC 3 cut(s) 9, 94, 178
BcnI CCSGG 2 cut(s) 275, 405
BfaI CTAG 1 cut(s) 171
BfoI RGCGCY 1 cut(s) 194
BisI GCNGC 1 cut(s) 345
BlsI GCNGC 1 cut(s) 346
Bme1390I CCNGG 2 cut(s) 275, 405
BmgT120I GGNCC 2 cut(s) 393, 394
BmiI GGNNCC 7 cut(s) 96, 105, 114, 159, 192, 394, 395
BmrFI CCNGG 2 cut(s) 275, 405
BpiI GAAGAC 1 cut(s) 389
BplI GAGNNNNNCTC 2 cut(s) 344, 376
BpmI CTGGAG 1 cut(s) 300
BpuMI CCSGG 2 cut(s) 275, 405
BsaHI GRCGYC 1 cut(s) 191
BsaJI CCNNGG 3 cut(s) 134, 243, 404
Bsc4I CCNNNNNNNGG 2 cut(s) 302, 403
Bse118I RCCGGY 2 cut(s) 91, 215
Bse1I ACTGG 1 cut(s) 432
BseDI CCNNGG 3 cut(s) 134, 243, 404
BseGI GGATG 1 cut(s) 394
BseLI CCNNNNNNNGG 2 cut(s) 302, 403
BseMII CTCAG 1 cut(s) 354
BseNI ACTGG 1 cut(s) 432
BseSI GKGCMC 2 cut(s) 251, 397
BseXI GCAGC 1 cut(s) 356
Bsh1285I CGRYCG 1 cut(s) 315
BshFI GGCC 2 cut(s) 215, 395
BshNI GGYRCC 1 cut(s) 190
BsiEI CGRYCG 1 cut(s) 315
BsiHKAI GWGCWC 1 cut(s) 71
BsiSI CCGG 4 cut(s) 92, 216, 275, 404
BsiWI CGTACG 1 cut(s) 228
BslI CCNNNNNNNGG 2 cut(s) 302, 403
BsnI GGCC 2 cut(s) 215, 395
Bsp120I GGGCCC 1 cut(s) 393
Bsp1286I GDGCHC 4 cut(s) 71, 142, 251, 397
Bsp143I GATC 1 cut(s) 312
BspACI CCGC 2 cut(s) 296, 302
BspANI GGCC 2 cut(s) 215, 395
BspCNI CTCAG 1 cut(s) 355
BspLI GGNNCC 7 cut(s) 96, 105, 114, 159, 192, 394, 395
BspT107I GGYRCC 1 cut(s) 190
BsrFI RCCGGY 2 cut(s) 91, 215
BsrI ACTGG 1 cut(s) 432
BssAI RCCGGY 2 cut(s) 91, 215
BssECI CCNNGG 3 cut(s) 134, 243, 404
BssMI GATC 1 cut(s) 312
BssNI GRCGYC 1 cut(s) 191
BssT1I CCWWGG 2 cut(s) 134, 243
Bst4CI ACNGT 2 cut(s) 255, 453
BstACI GRCGYC 1 cut(s) 191
BstC8I GCNNGC 3 cut(s) 217, 300, 373
BstDEI CTNAG 1 cut(s) 363
BstF5I GGATG 1 cut(s) 394
BstH2I RGCGCY 1 cut(s) 194
BstHHI GCGC 3 cut(s) 193, 344, 349
BstKTI GATC 1 cut(s) 315
BstMBI GATC 1 cut(s) 312
BstMCI CGRYCG 1 cut(s) 315
BstMWI GCNNNNNNNGC 4 cut(s) 55, 101, 110, 164
BstSCI CCNGG 2 cut(s) 273, 403
BstSLI GKGCMC 2 cut(s) 251, 397
BstV1I GCAGC 1 cut(s) 356
BstV2I GAAGAC 1 cut(s) 389
BsuRI GGCC 2 cut(s) 215, 395
BtsCI GGATG 1 cut(s) 394
BtsIMutI CAGTG 2 cut(s) 251, 458
Cac8I GCNNGC 3 cut(s) 217, 300, 373
CfoI GCGC 3 cut(s) 193, 344, 349
Cfr10I RCCGGY 2 cut(s) 91, 215
Cfr13I GGNCC 2 cut(s) 393, 394
CseI GACGC 1 cut(s) 430
Csp6I GTAC 2 cut(s) 229, 336
CviAII CATG 1 cut(s) 223
CviQI GTAC 2 cut(s) 229, 336
DdeI CTNAG 1 cut(s) 363
DinI GGCGCC 1 cut(s) 192
DpnI GATC 1 cut(s) 314
DpnII GATC 1 cut(s) 312
DraI TTTAAA 1 cut(s) 85
EaeI YGGCCR 1 cut(s) 213
Ecl136II GAGCTC 1 cut(s) 69
Eco130I CCWWGG 2 cut(s) 134, 243
Eco24I GRGCYC 3 cut(s) 71, 142, 397
Eco53kI GAGCTC 1 cut(s) 69
Eco57I CTGAAG 1 cut(s) 401
EcoICRI GAGCTC 1 cut(s) 69
EcoO109I RGGNCCY 1 cut(s) 393
EcoT14I CCWWGG 2 cut(s) 134, 243
EcoT38I GRGCYC 3 cut(s) 71, 142, 397
EgeI GGCGCC 1 cut(s) 192
EheI GGCGCC 1 cut(s) 192
ErhI CCWWGG 2 cut(s) 134, 243
FaeI CATG 1 cut(s) 226
FaiI YATR 1 cut(s) 224
FatI CATG 1 cut(s) 222
FauI CCCGC 1 cut(s) 295
Fnu4HI GCNGC 1 cut(s) 345
FokI GGATG 1 cut(s) 401
FriOI GRGCYC 3 cut(s) 71, 142, 397
Fsp4HI GCNGC 1 cut(s) 345
FspBI CTAG 1 cut(s) 171
GlaI GCGC 3 cut(s) 192, 343, 348
GluI GCNGC 1 cut(s) 345
GsuI CTGGAG 1 cut(s) 300
HaeII RGCGCY 1 cut(s) 194
HaeIII GGCC 2 cut(s) 215, 395
HapII CCGG 4 cut(s) 92, 216, 275, 404
HgaI GACGC 1 cut(s) 430
HhaI GCGC 3 cut(s) 193, 344, 349
Hin1I GRCGYC 1 cut(s) 191
Hin1II CATG 1 cut(s) 226
Hin6I GCGC 3 cut(s) 191, 342, 347
HinP1I GCGC 3 cut(s) 191, 342, 347
HinfI GANTC 3 cut(s) 31, 317, 439
HpaII CCGG 4 cut(s) 92, 216, 275, 404
Hpy166II GTNNAC 1 cut(s) 415
Hpy188I TCNGA 5 cut(s) 99, 144, 264, 364, 444
Hpy188III TCNNGA 1 cut(s) 321
Hpy8I GTNNAC 1 cut(s) 415
Hpy99I CGWCG 3 cut(s) 130, 199, 424
HpyAV CCTTC 1 cut(s) 94
HpyCH4III ACNGT 2 cut(s) 255, 453
HpyCH4IV ACGT 1 cut(s) 128
HpyF10VI GCNNNNNNNGC 4 cut(s) 55, 101, 110, 164
HpyF3I CTNAG 1 cut(s) 363
HpySE526I ACGT 1 cut(s) 128
Hsp92I GRCGYC 1 cut(s) 191
Hsp92II CATG 1 cut(s) 226
HspAI GCGC 3 cut(s) 191, 342, 347
KasI GGCGCC 1 cut(s) 190
KroI GCCGGC 1 cut(s) 215
KroNI GCCGGC 1 cut(s) 217
Kzo9I GATC 1 cut(s) 312
LmnI GCTCC 6 cut(s) 100, 109, 118, 144, 163, 329
Lsp1109I GCAGC 1 cut(s) 356
MaeI CTAG 1 cut(s) 171
MaeII ACGT 1 cut(s) 128
MaeIII GTNAC 1 cut(s) 356
MalI GATC 1 cut(s) 314
MboI GATC 1 cut(s) 312
MboII GAAGA 3 cut(s) 144, 177, 394
MhlI GDGCHC 4 cut(s) 71, 142, 251, 397
MluCI AATT 1 cut(s) 4
Mly113I GGCGCC 1 cut(s) 191
MnlI CCTC 2 cut(s) 280, 358
MroNI GCCGGC 1 cut(s) 215
MseI TTAA 2 cut(s) 84, 239
MspA1I CMGCKG 1 cut(s) 298
MspI CCGG 4 cut(s) 92, 216, 275, 404
MspR9I CCNGG 2 cut(s) 275, 405
MteI GCGCNGCGC 1 cut(s) 345
MwoI GCNNNNNNNGC 4 cut(s) 55, 101, 110, 164
NaeI GCCGGC 1 cut(s) 217
NarI GGCGCC 1 cut(s) 191
NciI CCSGG 2 cut(s) 275, 405
NdeII GATC 1 cut(s) 312
NgoMIV GCCGGC 1 cut(s) 215
NlaIII CATG 1 cut(s) 226
NlaIV GGNNCC 7 cut(s) 96, 105, 114, 159, 192, 394, 395
PcsI WCGNNNNNNNCGW 2 cut(s) 194, 428
PdiI GCCGGC 1 cut(s) 217
PfeI GAWTC 3 cut(s) 31, 317, 439
Pfl23II CGTACG 1 cut(s) 228
PkrI GCNGC 1 cut(s) 346
Ple19I CGATCG 1 cut(s) 315
PluTI GGCGCC 1 cut(s) 194
Psp124BI GAGCTC 1 cut(s) 71
PspLI CGTACG 1 cut(s) 228
PspN4I GGNNCC 7 cut(s) 96, 105, 114, 159, 192, 394, 395
PspOMI GGGCCC 1 cut(s) 393
PspPI GGNCC 2 cut(s) 393, 394
PvuI CGATCG 1 cut(s) 315
RsaI GTAC 2 cut(s) 230, 337
RsaNI GTAC 2 cut(s) 229, 336
SacI GAGCTC 1 cut(s) 71
SaqAI TTAA 2 cut(s) 84, 239
SatI GCNGC 1 cut(s) 345
Sau3AI GATC 1 cut(s) 312
Sau96I GGNCC 2 cut(s) 393, 394
ScrFI CCNGG 2 cut(s) 275, 405
SduI GDGCHC 4 cut(s) 71, 142, 251, 397
SetI ASST 6 cut(s) 43, 71, 131, 334, 369, 377
SfoI GGCGCC 1 cut(s) 192
Sse9I AATT 1 cut(s) 4
SsiI CCGC 2 cut(s) 296, 302
SspDI GGCGCC 1 cut(s) 190
SspMI CTAG 1 cut(s) 171
SstI GAGCTC 1 cut(s) 71
StyD4I CCNGG 2 cut(s) 273, 403
StyI CCWWGG 2 cut(s) 134, 243
TaaI ACNGT 2 cut(s) 255, 453
TaiI ACGT 1 cut(s) 131
TaqI TCGA 4 cut(s) 74, 205, 270, 315
TasI AATT 1 cut(s) 4
TfiI GAWTC 3 cut(s) 31, 317, 439
Tru1I TTAA 2 cut(s) 84, 239
Tru9I TTAA 2 cut(s) 84, 239
TscAI CASTG 2 cut(s) 258, 458
TseI GCWGC 1 cut(s) 344
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 401
TspRI CASTG 2 cut(s) 258, 458
XapI RAATTY 1 cut(s) 4
XcmI CCANNNNNNNNNTGG 1 cut(s) 208
XspI CTAG 1 cut(s) 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.