pycom16g18650

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0010791
Physical Location & Seq
Reverse (-)
15389192 .. 15389641
450 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g18650.1

Sequence Viewer

Length: 375 bp
ATGAGTTTCCAGCAAAACCACGGCCGTAGGATTCTGCCACCTGTCCCGTCCAGAAAGCGAAAGGAGAGCTCATCGAACGGCTTTAAACAACCGGCTCCGAAGGCTCCAAAGGCTCCAAGCACAACGACGTTTCCAAAGGCTCCAAGCAAATCTTCGGCTCCAAAGGCTTCGAGCAAAACGACATCTTCGGCGCCGTCGTCCAATCGACTTCTGGCCGGCTACATGGCGTACGAGTATTTAACCAAGGGCACACTGTTAGGTCAGAAGTTCGACCCGGCTGGAGAAGAGGCAGTGACGAGCTTACTGAAGACGGATGGGGCCCACATTCCCGGGATTGTAAACCCGACGCAACTGGCGAAATGGATTCAGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

125

Amino Acids

13.28

Weight (kDa)

10.33

Isoelectric Point (pI)

67.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010791)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23440
fragaria_vesca FvH4_4g04100
malus_domestica MD13G1217500.v1.1 MD16G1222600.v1.1
prunus_persica Prupe.1G044300_v2.0.a1 Prupe.1G044300_v2.0.a1
pyrus_communis pycom13g19090 pycom16g18650
rosa_chinensis RchiOBHm_Chr4g0394481
rosa_laevigata RLG00000009695
rosa_multiflora Rmu_sc0000200.1_g000024 Rmu_sc0013311.1_g000001
rosa_roxburghii Rroxscaffold_5G00339510
rosa_rugosa Rorug03G0365800.1
rosa_samantha Rh4AG052300 Rh4BG050200 Rh4CG056600 Rh4DG049300
rosa_wichuraiana Rw0G003380 Rw4G004220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 190
AcoI YGGCCR 2 cut(s) 22, 213
AcuI CTGAAG 1 cut(s) 326
AcyI GRCGYC 1 cut(s) 191
AfaI GTAC 1 cut(s) 230
AjuI GAANNNNNNNTTGG 2 cut(s) 136, 168
AluBI AGCT 2 cut(s) 69, 300
AluI AGCT 2 cut(s) 69, 300
Alw21I GWGCWC 1 cut(s) 71
Ama87I CYCGRG 1 cut(s) 329
AoxI GGCC 3 cut(s) 22, 213, 318
ApaI GGGCCC 1 cut(s) 322
AspLEI GCGC 1 cut(s) 193
AspS9I GGNCC 2 cut(s) 318, 319
AsuC2I CCSGG 3 cut(s) 275, 330, 331
AvaI CYCGRG 1 cut(s) 329
BaeGI GKGCMC 2 cut(s) 251, 322
BanI GGYRCC 1 cut(s) 190
BanII GRGCYC 2 cut(s) 71, 322
BbsI GAAGAC 1 cut(s) 314
Bbv12I GWGCWC 1 cut(s) 71
BccI CCATC 1 cut(s) 308
BceAI ACGGC 4 cut(s) 9, 37, 94, 178
BcnI CCSGG 3 cut(s) 275, 330, 331
BfoI RGCGCY 1 cut(s) 194
Bme1390I CCNGG 3 cut(s) 275, 330, 331
BmeT110I CYCGRG 1 cut(s) 329
BmgT120I GGNCC 2 cut(s) 318, 319
BmiI GGNNCC 8 cut(s) 96, 105, 114, 141, 159, 192, 319, 320
BmrFI CCNGG 3 cut(s) 275, 330, 331
BpiI GAAGAC 1 cut(s) 314
BpmI CTGGAG 1 cut(s) 300
BpuMI CCSGG 3 cut(s) 275, 330, 331
BsaHI GRCGYC 1 cut(s) 191
BsaJI CCNNGG 3 cut(s) 19, 243, 329
Bse118I RCCGGY 2 cut(s) 91, 215
Bse1I ACTGG 1 cut(s) 357
BseDI CCNNGG 3 cut(s) 19, 243, 329
BseGI GGATG 1 cut(s) 319
BseNI ACTGG 1 cut(s) 357
BseSI GKGCMC 2 cut(s) 251, 322
BseX3I CGGCCG 1 cut(s) 22
Bsh1285I CGRYCG 1 cut(s) 25
BshFI GGCC 3 cut(s) 24, 215, 320
BshNI GGYRCC 1 cut(s) 190
BsiEI CGRYCG 1 cut(s) 25
BsiHKAI GWGCWC 1 cut(s) 71
BsiHKCI CYCGRG 1 cut(s) 329
BsiSI CCGG 4 cut(s) 92, 216, 275, 330
BsiWI CGTACG 1 cut(s) 228
BslFI GGGAC 1 cut(s) 29
BsmFI GGGAC 1 cut(s) 29
BsnI GGCC 3 cut(s) 24, 215, 320
BsoBI CYCGRG 1 cut(s) 329
Bsp120I GGGCCC 1 cut(s) 318
Bsp1286I GDGCHC 3 cut(s) 71, 251, 322
BspANI GGCC 3 cut(s) 24, 215, 320
BspLI GGNNCC 8 cut(s) 96, 105, 114, 141, 159, 192, 319, 320
BspT107I GGYRCC 1 cut(s) 190
BsrFI RCCGGY 2 cut(s) 91, 215
BsrI ACTGG 1 cut(s) 357
BssAI RCCGGY 2 cut(s) 91, 215
BssECI CCNNGG 3 cut(s) 19, 243, 329
BssNI GRCGYC 1 cut(s) 191
BssT1I CCWWGG 1 cut(s) 243
Bst4CI ACNGT 1 cut(s) 255
Bst6I CTCTTC 1 cut(s) 279
BstACI GRCGYC 1 cut(s) 191
BstC8I GCNNGC 1 cut(s) 217
BstDSI CCRYGG 1 cut(s) 19
BstF5I GGATG 1 cut(s) 319
BstH2I RGCGCY 1 cut(s) 194
BstHHI GCGC 1 cut(s) 193
BstMCI CGRYCG 1 cut(s) 25
BstMWI GCNNNNNNNGC 3 cut(s) 101, 110, 164
BstSCI CCNGG 3 cut(s) 273, 328, 329
BstSLI GKGCMC 2 cut(s) 251, 322
BstV2I GAAGAC 1 cut(s) 314
BstZI CGGCCG 1 cut(s) 22
BsuRI GGCC 3 cut(s) 24, 215, 320
BtgI CCRYGG 1 cut(s) 19
BtsCI GGATG 1 cut(s) 319
BtsI GCAGTG 1 cut(s) 297
BtsIMutI CAGTG 2 cut(s) 251, 297
Cac8I GCNNGC 1 cut(s) 217
CfoI GCGC 1 cut(s) 193
Cfr10I RCCGGY 2 cut(s) 91, 215
Cfr13I GGNCC 2 cut(s) 318, 319
Cfr9I CCCGGG 1 cut(s) 329
CseI GACGC 1 cut(s) 355
Csp6I GTAC 1 cut(s) 229
CviAII CATG 1 cut(s) 223
CviQI GTAC 1 cut(s) 229
DinI GGCGCC 1 cut(s) 192
DraI TTTAAA 1 cut(s) 85
EaeI YGGCCR 2 cut(s) 22, 213
EagI CGGCCG 1 cut(s) 22
Eam1104I CTCTTC 1 cut(s) 279
EarI CTCTTC 1 cut(s) 279
Ecl136II GAGCTC 1 cut(s) 69
EclXI CGGCCG 1 cut(s) 22
Eco130I CCWWGG 1 cut(s) 243
Eco24I GRGCYC 2 cut(s) 71, 322
Eco52I CGGCCG 1 cut(s) 22
Eco53kI GAGCTC 1 cut(s) 69
Eco57I CTGAAG 1 cut(s) 326
Eco88I CYCGRG 1 cut(s) 329
EcoICRI GAGCTC 1 cut(s) 69
EcoO109I RGGNCCY 1 cut(s) 318
EcoT14I CCWWGG 1 cut(s) 243
EcoT38I GRGCYC 2 cut(s) 71, 322
EgeI GGCGCC 1 cut(s) 192
EheI GGCGCC 1 cut(s) 192
ErhI CCWWGG 1 cut(s) 243
FaeI CATG 1 cut(s) 226
FaiI YATR 1 cut(s) 224
FalI AAGNNNNNCTT 2 cut(s) 136, 168
FaqI GGGAC 1 cut(s) 29
FatI CATG 1 cut(s) 222
FokI GGATG 1 cut(s) 326
FriOI GRGCYC 2 cut(s) 71, 322
GlaI GCGC 1 cut(s) 192
GsuI CTGGAG 1 cut(s) 300
HaeII RGCGCY 1 cut(s) 194
HaeIII GGCC 3 cut(s) 24, 215, 320
HapII CCGG 4 cut(s) 92, 216, 275, 330
HgaI GACGC 1 cut(s) 355
HhaI GCGC 1 cut(s) 193
Hin1I GRCGYC 1 cut(s) 191
Hin1II CATG 1 cut(s) 226
Hin6I GCGC 1 cut(s) 191
HinP1I GCGC 1 cut(s) 191
HinfI GANTC 2 cut(s) 31, 364
HpaII CCGG 4 cut(s) 92, 216, 275, 330
Hpy166II GTNNAC 1 cut(s) 340
Hpy188I TCNGA 3 cut(s) 99, 264, 369
Hpy188III TCNNGA 1 cut(s) 51
Hpy8I GTNNAC 1 cut(s) 340
Hpy99I CGWCG 3 cut(s) 130, 199, 349
HpyAV CCTTC 1 cut(s) 94
HpyCH4III ACNGT 1 cut(s) 255
HpyCH4IV ACGT 1 cut(s) 128
HpyF10VI GCNNNNNNNGC 3 cut(s) 101, 110, 164
HpySE526I ACGT 1 cut(s) 128
Hsp92I GRCGYC 1 cut(s) 191
Hsp92II CATG 1 cut(s) 226
HspAI GCGC 1 cut(s) 191
KasI GGCGCC 1 cut(s) 190
KroI GCCGGC 1 cut(s) 215
KroNI GCCGGC 1 cut(s) 217
LmnI GCTCC 5 cut(s) 100, 109, 118, 145, 163
MaeII ACGT 1 cut(s) 128
MaeIII GTNAC 1 cut(s) 292
MboII GAAGA 4 cut(s) 144, 177, 296, 319
MhlI GDGCHC 3 cut(s) 71, 251, 322
Mly113I GGCGCC 1 cut(s) 191
MnlI CCTC 1 cut(s) 280
MroNI GCCGGC 1 cut(s) 215
MseI TTAA 2 cut(s) 84, 239
MspI CCGG 4 cut(s) 92, 216, 275, 330
MspR9I CCNGG 3 cut(s) 275, 330, 331
MwoI GCNNNNNNNGC 3 cut(s) 101, 110, 164
NaeI GCCGGC 1 cut(s) 217
NarI GGCGCC 1 cut(s) 191
NciI CCSGG 3 cut(s) 275, 330, 331
NgoMIV GCCGGC 1 cut(s) 215
NlaIII CATG 1 cut(s) 226
NlaIV GGNNCC 8 cut(s) 96, 105, 114, 141, 159, 192, 319, 320
NmuCI GTSAC 1 cut(s) 292
PcsI WCGNNNNNNNCGW 3 cut(s) 176, 194, 353
PdiI GCCGGC 1 cut(s) 217
PfeI GAWTC 2 cut(s) 31, 364
Pfl23II CGTACG 1 cut(s) 228
PluTI GGCGCC 1 cut(s) 194
Psp124BI GAGCTC 1 cut(s) 71
PspLI CGTACG 1 cut(s) 228
PspN4I GGNNCC 8 cut(s) 96, 105, 114, 141, 159, 192, 319, 320
PspOMI GGGCCC 1 cut(s) 318
PspPI GGNCC 2 cut(s) 318, 319
RsaI GTAC 1 cut(s) 230
RsaNI GTAC 1 cut(s) 229
SacI GAGCTC 1 cut(s) 71
SaqAI TTAA 2 cut(s) 84, 239
Sau96I GGNCC 2 cut(s) 318, 319
ScrFI CCNGG 3 cut(s) 275, 330, 331
SduI GDGCHC 3 cut(s) 71, 251, 322
SetI ASST 5 cut(s) 43, 71, 131, 262, 302
SfoI GGCGCC 1 cut(s) 192
SmaI CCCGGG 1 cut(s) 331
SspDI GGCGCC 1 cut(s) 190
SstI GAGCTC 1 cut(s) 71
StyD4I CCNGG 3 cut(s) 273, 328, 329
StyI CCWWGG 1 cut(s) 243
TaaI ACNGT 1 cut(s) 255
TaiI ACGT 1 cut(s) 131
TaqI TCGA 4 cut(s) 74, 170, 205, 270
TfiI GAWTC 2 cut(s) 31, 364
Tru1I TTAA 2 cut(s) 84, 239
Tru9I TTAA 2 cut(s) 84, 239
TscAI CASTG 2 cut(s) 258, 297
TseFI GTSAC 1 cut(s) 292
Tsp45I GTSAC 1 cut(s) 292
TspGWI ACGGA 1 cut(s) 326
TspMI CCCGGG 1 cut(s) 329
TspRI CASTG 2 cut(s) 258, 297
XcmI CCANNNNNNNNNTGG 1 cut(s) 208
XmaI CCCGGG 1 cut(s) 329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.