FvH4_4g05050

AWPM-19-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0012496
Physical Location & Seq
Forward (+)
4365050 .. 4366264
1215 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g05050.t2

Sequence Viewer

Length: 543 bp
ATGGCGACGGTGGGAAGAAACATAGCAGCTCCTTTGCTGTTTCTCAACTTGATCATGTACTTGATCGTAGTAGGGTTTGCTAGTTGGTGCCTTAACAGGTTCATCAATGGCCAGACCAACCACCCGAGTTTTGGAGGAAATGGTGCAACGATGTTCTTCCTGATCTTTTCCATAATGGCTGGTGTGATGGGAGTAGCGTCGAAGTTGGCTGGAGCAGGCCATCTCAGAGCCTGGAGGAGTGACAGTCTAGCTGCAGCAGGATCTTCATCAATTGTGGCTTGGGCTCTCACTGCACTAGCCTTTGGACTCGCATGCAAGCAAATCAATGTTGGAGGATACAGGGGATGGAGGCTGAGGGTTTTGGAGGCTTTCATAATAATCTTGACATTCACCCAGCTTTTGTATCTCTTGCTAATCCATGCTGGTATCTTCAGCAGCAAGTATGGTCCTGGTTACCGTGACACCGACTACCCTACAGCTGCCGGCGGTGTCGATCCAATCCACAAGGGCGGAACTGCAGTCCCTGCTTCTAGGGTGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AWPM-19

Protein Analysis

181

Amino Acids

19.11

Weight (kDa)

10.01

Isoelectric Point (pI)

28.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AWPM-19 PF05512 1 - 92 6.5e-41 AWPM-19-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012496)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04560
fragaria_vesca FvH4_4g05050 FvH4_4g05050
malus_domestica MD13G1224200.v1.1 MD16G1229000.v1.1
prunus_persica Prupe.1G050900_v2.0.a1 Prupe.1G050900_v2.0.a1
pyrus_communis pycom13g19800
rosa_chinensis RchiOBHm_Chr4g0395971
rosa_laevigata RLG00000009584
rosa_multiflora Rmu_sc0001103.1_g000004
rosa_roxburghii Rroxscaffold_5G00340730
rosa_rugosa Rorug03G0353100
rosa_samantha Rh4AG064300 Rh4AG066300 Rh4BG063000 Rh4CG069400 Rh4DG059500
rosa_wichuraiana Rw4G005190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 87
AciI CCGC 2 cut(s) 486, 510
AclWI GGATC 2 cut(s) 268, 488
AcoI YGGCCR 1 cut(s) 109
AcuI CTGAAG 1 cut(s) 415
AfaI GTAC 1 cut(s) 59
AfiI CCNNNNNNNGG 1 cut(s) 131
AjnI CCWGG 2 cut(s) 230, 448
AluBI AGCT 4 cut(s) 29, 251, 397, 479
AluI AGCT 4 cut(s) 29, 251, 397, 479
AlwI GGATC 2 cut(s) 268, 488
AlwNI CAGNNNCTG 2 cut(s) 231, 524
Ama87I CYCGRG 1 cut(s) 124
AoxI GGCC 2 cut(s) 109, 217
ApeKI GCWGC 5 cut(s) 26, 251, 254, 435, 479
AspS9I GGNCC 1 cut(s) 446
AsuHPI GGTGA 1 cut(s) 382
AvaI CYCGRG 1 cut(s) 124
AvaII GGWCC 1 cut(s) 446
BalI TGGCCA 1 cut(s) 111
BanI GGYRCC 1 cut(s) 87
BanII GRGCYC 1 cut(s) 286
BbvCI CCTCAGC 1 cut(s) 353
BbvI GCAGC 5 cut(s) 38, 238, 266, 447, 466
BccI CCATC 3 cut(s) 181, 228, 339
BciT130I CCWGG 2 cut(s) 232, 450
BciVI GTATCC 1 cut(s) 329
BclI TGATCA 1 cut(s) 51
BfaI CTAG 4 cut(s) 81, 248, 296, 531
BfmI CTRYAG 3 cut(s) 252, 474, 516
BfuI GTATCC 1 cut(s) 329
BisI GCNGC 5 cut(s) 27, 252, 255, 436, 480
BlsI GCNGC 5 cut(s) 28, 253, 256, 437, 481
Bme1390I CCNGG 2 cut(s) 232, 450
Bme18I GGWCC 1 cut(s) 446
BmeT110I CYCGRG 1 cut(s) 124
BmgT120I GGNCC 1 cut(s) 446
BmiI GGNNCC 1 cut(s) 89
BmrFI CCNGG 2 cut(s) 232, 450
BpmI CTGGAG 2 cut(s) 231, 253
Bpu10I CCTNAGC 1 cut(s) 353
BsaBI GATNNNNATC 1 cut(s) 265
BsaXI ACNNNNNCTCC 2 cut(s) 229, 259
Bsc4I CCNNNNNNNGG 1 cut(s) 131
Bse118I RCCGGY 1 cut(s) 482
Bse8I GATNNNNATC 1 cut(s) 265
BseBI CCWGG 2 cut(s) 232, 450
BseGI GGATG 1 cut(s) 350
BseJI GATNNNNATC 1 cut(s) 265
BseLI CCNNNNNNNGG 1 cut(s) 131
BseMII CTCAG 2 cut(s) 238, 344
BseRI GAGGAG 1 cut(s) 250
BseXI GCAGC 5 cut(s) 38, 238, 266, 447, 466
BseYI CCCAGC 1 cut(s) 393
BsgI GTGCAG 1 cut(s) 276
BshFI GGCC 2 cut(s) 111, 219
BshNI GGYRCC 1 cut(s) 87
BsiHKCI CYCGRG 1 cut(s) 124
BsiSI CCGG 1 cut(s) 483
BslFI GGGAC 1 cut(s) 506
BslI CCNNNNNNNGG 1 cut(s) 131
BsmFI GGGAC 1 cut(s) 506
BsnI GGCC 2 cut(s) 111, 219
BsoBI CYCGRG 1 cut(s) 124
Bsp1286I GDGCHC 1 cut(s) 286
Bsp143I GATC 5 cut(s) 51, 63, 162, 260, 493
BspACI CCGC 2 cut(s) 486, 510
BspANI GGCC 2 cut(s) 111, 219
BspCNI CTCAG 2 cut(s) 237, 345
BspLI GGNNCC 1 cut(s) 89
BspMAI CTGCAG 2 cut(s) 256, 520
BspPI GGATC 2 cut(s) 268, 488
BspT107I GGYRCC 1 cut(s) 87
BsrFI RCCGGY 1 cut(s) 482
BssAI RCCGGY 1 cut(s) 482
BssMI GATC 5 cut(s) 51, 63, 162, 260, 493
Bst2UI CCWGG 2 cut(s) 232, 450
Bst4CI ACNGT 3 cut(s) 10, 245, 458
BstAPI GCANNNNNTGC 1 cut(s) 524
BstC8I GCNNGC 4 cut(s) 217, 313, 317, 484
BstDEI CTNAG 2 cut(s) 224, 353
BstEII GGTNACC 1 cut(s) 452
BstF5I GGATG 1 cut(s) 350
BstKTI GATC 5 cut(s) 54, 66, 165, 263, 496
BstMBI GATC 5 cut(s) 51, 63, 162, 260, 493
BstMWI GCNNNNNNNGC 2 cut(s) 290, 524
BstNI CCWGG 2 cut(s) 232, 450
BstNSI RCATGY 1 cut(s) 315
BstPI GGTNACC 1 cut(s) 452
BstSCI CCNGG 2 cut(s) 230, 448
BstSFI CTRYAG 3 cut(s) 252, 474, 516
BstV1I GCAGC 5 cut(s) 38, 238, 266, 447, 466
BstX2I RGATCY 1 cut(s) 260
BstYI RGATCY 1 cut(s) 260
BsuI GTATCC 1 cut(s) 329
BsuRI GGCC 2 cut(s) 111, 219
BtsCI GGATG 1 cut(s) 350
BtsI GCAGTG 1 cut(s) 288
BtsIMutI CAGTG 1 cut(s) 288
Cac8I GCNNGC 4 cut(s) 217, 313, 317, 484
CaiI CAGNNNCTG 2 cut(s) 231, 524
Cfr10I RCCGGY 1 cut(s) 482
Cfr13I GGNCC 1 cut(s) 446
CseI GACGC 1 cut(s) 186
Csp6I GTAC 1 cut(s) 58
CviAII CATG 3 cut(s) 55, 312, 419
CviQI GTAC 1 cut(s) 58
DdeI CTNAG 2 cut(s) 224, 353
DpnI GATC 5 cut(s) 53, 65, 164, 262, 495
DpnII GATC 5 cut(s) 51, 63, 162, 260, 493
EaeI YGGCCR 1 cut(s) 109
EciI GGCGGA 1 cut(s) 525
Eco24I GRGCYC 1 cut(s) 286
Eco47I GGWCC 1 cut(s) 446
Eco57I CTGAAG 1 cut(s) 415
Eco88I CYCGRG 1 cut(s) 124
Eco91I GGTNACC 1 cut(s) 452
EcoO65I GGTNACC 1 cut(s) 452
EcoRII CCWGG 2 cut(s) 230, 448
EcoT38I GRGCYC 1 cut(s) 286
FaeI CATG 3 cut(s) 58, 315, 422
FaiI YATR 7 cut(s) 23, 56, 173, 313, 374, 420, 444
FaqI GGGAC 1 cut(s) 506
FatI CATG 3 cut(s) 54, 311, 418
FbaI TGATCA 1 cut(s) 51
Fnu4HI GCNGC 5 cut(s) 27, 252, 255, 436, 480
FokI GGATG 1 cut(s) 357
FriOI GRGCYC 1 cut(s) 286
Fsp4HI GCNGC 5 cut(s) 27, 252, 255, 436, 480
FspBI CTAG 4 cut(s) 81, 248, 296, 531
GluI GCNGC 5 cut(s) 27, 252, 255, 436, 480
GsaI CCCAGC 1 cut(s) 397
GsuI CTGGAG 2 cut(s) 231, 253
HaeIII GGCC 2 cut(s) 111, 219
HapII CCGG 1 cut(s) 483
HgaI GACGC 1 cut(s) 186
Hin1II CATG 3 cut(s) 58, 315, 422
HinfI GANTC 1 cut(s) 306
HpaII CCGG 1 cut(s) 483
HphI GGTGA 1 cut(s) 382
Hpy188I TCNGA 1 cut(s) 227
Hpy188III TCNNGA 2 cut(s) 160, 382
Hpy99I CGWCG 2 cut(s) 10, 202
HpyCH4III ACNGT 3 cut(s) 10, 245, 458
HpyCH4V TGCA 5 cut(s) 146, 254, 293, 315, 518
HpyF10VI GCNNNNNNNGC 2 cut(s) 290, 524
HpyF3I CTNAG 2 cut(s) 224, 353
Hsp92II CATG 3 cut(s) 58, 315, 422
KroI GCCGGC 1 cut(s) 482
KroNI GCCGGC 1 cut(s) 484
Ksp22I TGATCA 1 cut(s) 51
Kzo9I GATC 5 cut(s) 51, 63, 162, 260, 493
LmnI GCTCC 2 cut(s) 34, 212
Lsp1109I GCAGC 5 cut(s) 38, 238, 266, 447, 466
MaeI CTAG 4 cut(s) 81, 248, 296, 531
MaeIII GTNAC 3 cut(s) 239, 452, 458
MalI GATC 5 cut(s) 53, 65, 164, 262, 495
MboI GATC 5 cut(s) 51, 63, 162, 260, 493
MboII GAAGA 4 cut(s) 27, 148, 255, 421
MfeI CAATTG 1 cut(s) 270
MflI RGATCY 1 cut(s) 260
MhlI GDGCHC 1 cut(s) 286
MlsI TGGCCA 1 cut(s) 111
MluCI AATT 1 cut(s) 270
MluNI TGGCCA 1 cut(s) 111
MlyI GAGTC 1 cut(s) 300
MmeI TCCRAC 1 cut(s) 310
MnlI CCTC 6 cut(s) 128, 228, 326, 342, 348, 358
Mox20I TGGCCA 1 cut(s) 111
MroNI GCCGGC 1 cut(s) 482
MscI TGGCCA 1 cut(s) 111
MseI TTAA 1 cut(s) 93
Msp20I TGGCCA 1 cut(s) 111
MspA1I CMGCKG 1 cut(s) 479
MspI CCGG 1 cut(s) 483
MspR9I CCNGG 2 cut(s) 232, 450
MunI CAATTG 1 cut(s) 270
MvaI CCWGG 2 cut(s) 232, 450
MwoI GCNNNNNNNGC 2 cut(s) 290, 524
NaeI GCCGGC 1 cut(s) 484
NdeII GATC 5 cut(s) 51, 63, 162, 260, 493
NgoMIV GCCGGC 1 cut(s) 482
NlaIII CATG 3 cut(s) 58, 315, 422
NlaIV GGNNCC 1 cut(s) 89
NmuCI GTSAC 2 cut(s) 239, 458
NspI RCATGY 1 cut(s) 315
PaeI GCATGC 1 cut(s) 315
PdiI GCCGGC 1 cut(s) 484
PkrI GCNGC 5 cut(s) 28, 253, 256, 437, 481
PleI GAGTC 1 cut(s) 300
PpsI GAGTC 1 cut(s) 300
Psp6I CCWGG 2 cut(s) 230, 448
PspEI GGTNACC 1 cut(s) 452
PspFI CCCAGC 1 cut(s) 393
PspGI CCWGG 2 cut(s) 230, 448
PspN4I GGNNCC 1 cut(s) 89
PspPI GGNCC 1 cut(s) 446
PstI CTGCAG 2 cut(s) 256, 520
PstNI CAGNNNCTG 2 cut(s) 231, 524
PsuI RGATCY 1 cut(s) 260
PvuII CAGCTG 1 cut(s) 479
RsaI GTAC 1 cut(s) 59
RsaNI GTAC 1 cut(s) 58
SaqAI TTAA 1 cut(s) 93
SatI GCNGC 5 cut(s) 27, 252, 255, 436, 480
Sau3AI GATC 5 cut(s) 51, 63, 162, 260, 493
Sau96I GGNCC 1 cut(s) 446
SchI GAGTC 1 cut(s) 300
ScrFI CCNGG 2 cut(s) 232, 450
SduI GDGCHC 1 cut(s) 286
SetI ASST 5 cut(s) 31, 101, 253, 399, 481
SfcI CTRYAG 3 cut(s) 252, 474, 516
SinI GGWCC 1 cut(s) 446
SphI GCATGC 1 cut(s) 315
Sse9I AATT 1 cut(s) 270
SsiI CCGC 2 cut(s) 486, 510
SspMI CTAG 4 cut(s) 81, 248, 296, 531
StyD4I CCNGG 2 cut(s) 230, 448
TaaI ACNGT 3 cut(s) 10, 245, 458
TaqI TCGA 2 cut(s) 200, 492
TasI AATT 1 cut(s) 270
TatI WGTACW 1 cut(s) 57
Tru1I TTAA 1 cut(s) 93
Tru9I TTAA 1 cut(s) 93
TscAI CASTG 1 cut(s) 295
TseFI GTSAC 2 cut(s) 239, 458
TseI GCWGC 5 cut(s) 26, 251, 254, 435, 479
Tsp45I GTSAC 2 cut(s) 239, 458
TspDTI ATGAA 3 cut(s) 91, 255, 361
TspRI CASTG 1 cut(s) 295
VpaK11BI GGWCC 1 cut(s) 446
XceI RCATGY 1 cut(s) 315
XcmI CCANNNNNNNNNTGG 1 cut(s) 128
XspI CTAG 4 cut(s) 81, 248, 296, 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.