MD16G1229000.v1.1

AWPM-19-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0012496
Physical Location & Seq
Forward (+)
23391455 .. 23392589
1135 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1229000.v1.1.491

Sequence Viewer

Length: 540 bp
ATGGCTCAGACTGTGGGAAGGAACGTGGCAGCTCCATTGCTGTTTCTGAACTTGATCATGTATTTGATCGTTGTCGGTTTTGCTAGTTGGTGTCTCAACAGGTTCATCAATGGCCAGACCAATCACCCTAGTTTTGGAGGAAATGGTGCAACAATGTTCTTTTTGATATTCTCCATATTGGCTGGAGTGATGGGCATAGCATCAAAGCTGGCCGGAGCAAACCACATCAGAGCTTGGAGGAGTGATAGCCTAGCTGCAGCAGGATCATCAGCCATCGTGGCATGGGCTCTCACAGCACTGGCCCTTGGGCTGGCATGCAAGCAGATAAGCATTGGAGGGTTTAGAGGATGGAGGCTGAGGGTCTTGGAGGCTTTCGTAATAGTCTTGACAGTCACTCAGCTATTGTACCTCTTGCTGCTTCATGCGGGTGTCTTCAGCAGCAAGTATGGCCCGGGCTACCGTGACACCGACTACCCGGCTGCCGGCGATCCGATACACAAGGGCACAACTGGAGGAGTTCCTGCGTCTAGGGTTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AWPM-19

Protein Analysis

180

Amino Acids

18.94

Weight (kDa)

10.1

Isoelectric Point (pI)

25.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AWPM-19 PF05512 15 - 143 7.8e-54 AWPM-19-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012496)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04560
fragaria_vesca FvH4_4g05050 FvH4_4g05050
malus_domestica MD13G1224200.v1.1 MD16G1229000.v1.1
prunus_persica Prupe.1G050900_v2.0.a1 Prupe.1G050900_v2.0.a1
pyrus_communis pycom13g19800
rosa_chinensis RchiOBHm_Chr4g0395971
rosa_laevigata RLG00000009584
rosa_multiflora Rmu_sc0001103.1_g000004
rosa_roxburghii Rroxscaffold_5G00340730
rosa_rugosa Rorug03G0353100
rosa_samantha Rh4AG064300 Rh4AG066300 Rh4BG063000 Rh4CG069400 Rh4DG059500
rosa_wichuraiana Rw4G005190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 425
AclWI GGATC 2 cut(s) 271, 482
AcoI YGGCCR 2 cut(s) 112, 210
AcuI CTGAAG 1 cut(s) 418
AfaI GTAC 1 cut(s) 407
AfiI CCNNNNNNNGG 3 cut(s) 134, 310, 482
AluBI AGCT 5 cut(s) 32, 208, 233, 254, 400
AluI AGCT 5 cut(s) 32, 208, 233, 254, 400
Alw26I GTCTC 1 cut(s) 98
AlwI GGATC 2 cut(s) 271, 482
Ama87I CYCGRG 1 cut(s) 451
AoxI GGCC 4 cut(s) 112, 210, 300, 448
ApeKI GCWGC 6 cut(s) 29, 254, 257, 415, 438, 479
AspS9I GGNCC 2 cut(s) 301, 449
AsuC2I CCSGG 3 cut(s) 452, 453, 476
AsuHPI GGTGA 1 cut(s) 116
AvaI CYCGRG 1 cut(s) 451
BaeGI GKGCMC 1 cut(s) 506
BalI TGGCCA 1 cut(s) 114
BanII GRGCYC 1 cut(s) 289
BbsI GAAGAC 1 cut(s) 424
BbvCI CCTCAGC 1 cut(s) 356
BbvI GCAGC 6 cut(s) 41, 241, 269, 402, 450, 466
BccI CCATC 3 cut(s) 184, 281, 342
BclI TGATCA 1 cut(s) 54
BcnI CCSGG 3 cut(s) 452, 453, 476
BcoDI GTCTC 1 cut(s) 98
BfaI CTAG 4 cut(s) 84, 129, 251, 528
BfmI CTRYAG 1 cut(s) 255
BglI GCCNNNNNGGC 1 cut(s) 278
BisI GCNGC 6 cut(s) 30, 255, 258, 416, 439, 480
BlsI GCNGC 6 cut(s) 31, 256, 259, 417, 440, 481
Bme1390I CCNGG 3 cut(s) 452, 453, 476
BmeT110I CYCGRG 1 cut(s) 451
BmgT120I GGNCC 2 cut(s) 301, 449
BmrFI CCNGG 3 cut(s) 452, 453, 476
BmsI GCATC 1 cut(s) 209
BpiI GAAGAC 1 cut(s) 424
BpmI CTGGAG 2 cut(s) 204, 531
Bpu10I CCTNAGC 1 cut(s) 356
BpuMI CCSGG 3 cut(s) 452, 453, 476
BsaJI CCNNGG 2 cut(s) 304, 451
Bsc4I CCNNNNNNNGG 3 cut(s) 134, 310, 482
Bse118I RCCGGY 1 cut(s) 482
Bse1I ACTGG 2 cut(s) 303, 514
Bse3DI GCAATG 1 cut(s) 35
BseDI CCNNGG 2 cut(s) 304, 451
BseGI GGATG 1 cut(s) 353
BseLI CCNNNNNNNGG 3 cut(s) 134, 310, 482
BseMI GCAATG 1 cut(s) 35
BseMII CTCAG 3 cut(s) 20, 347, 410
BseNI ACTGG 2 cut(s) 303, 514
BseRI GAGGAG 2 cut(s) 253, 528
BseSI GKGCMC 1 cut(s) 506
BseXI GCAGC 6 cut(s) 41, 241, 269, 402, 450, 466
BshFI GGCC 4 cut(s) 114, 212, 302, 450
BsiHKCI CYCGRG 1 cut(s) 451
BsiSI CCGG 4 cut(s) 213, 452, 476, 483
BslI CCNNNNNNNGG 3 cut(s) 134, 310, 482
BsmAI GTCTC 1 cut(s) 98
BsnI GGCC 4 cut(s) 114, 212, 302, 450
BsoBI CYCGRG 1 cut(s) 451
Bsp1286I GDGCHC 2 cut(s) 289, 506
Bsp143I GATC 4 cut(s) 54, 66, 263, 487
BspACI CCGC 1 cut(s) 425
BspANI GGCC 4 cut(s) 114, 212, 302, 450
BspCNI CTCAG 3 cut(s) 19, 348, 409
BspMAI CTGCAG 1 cut(s) 259
BspPI GGATC 2 cut(s) 271, 482
BsrDI GCAATG 1 cut(s) 35
BsrFI RCCGGY 1 cut(s) 482
BsrI ACTGG 2 cut(s) 303, 514
BssAI RCCGGY 1 cut(s) 482
BssECI CCNNGG 2 cut(s) 304, 451
BssMI GATC 4 cut(s) 54, 66, 263, 487
BssT1I CCWWGG 1 cut(s) 304
Bst4CI ACNGT 3 cut(s) 13, 391, 461
BstC8I GCNNGC 5 cut(s) 210, 312, 316, 320, 484
BstDEI CTNAG 3 cut(s) 6, 356, 396
BstF5I GGATG 1 cut(s) 353
BstKTI GATC 4 cut(s) 57, 69, 266, 490
BstMAI GTCTC 1 cut(s) 98
BstMBI GATC 4 cut(s) 54, 66, 263, 487
BstMWI GCNNNNNNNGC 3 cut(s) 278, 293, 447
BstNSI RCATGY 1 cut(s) 318
BstSCI CCNGG 3 cut(s) 450, 451, 474
BstSFI CTRYAG 1 cut(s) 255
BstSLI GKGCMC 1 cut(s) 506
BstV1I GCAGC 6 cut(s) 41, 241, 269, 402, 450, 466
BstV2I GAAGAC 1 cut(s) 424
BsuRI GGCC 4 cut(s) 114, 212, 302, 450
BtsCI GGATG 1 cut(s) 353
BtsIMutI CAGTG 1 cut(s) 296
Cac8I GCNNGC 5 cut(s) 210, 312, 316, 320, 484
Cfr10I RCCGGY 1 cut(s) 482
Cfr13I GGNCC 2 cut(s) 301, 449
Cfr9I CCCGGG 1 cut(s) 451
CseI GACGC 1 cut(s) 513
Csp6I GTAC 1 cut(s) 406
CviAII CATG 4 cut(s) 58, 282, 315, 422
CviQI GTAC 1 cut(s) 406
DdeI CTNAG 3 cut(s) 6, 356, 396
DpnI GATC 4 cut(s) 56, 68, 265, 489
DpnII GATC 4 cut(s) 54, 66, 263, 487
EaeI YGGCCR 2 cut(s) 112, 210
Eco130I CCWWGG 1 cut(s) 304
Eco24I GRGCYC 1 cut(s) 289
Eco57I CTGAAG 1 cut(s) 418
Eco88I CYCGRG 1 cut(s) 451
EcoT14I CCWWGG 1 cut(s) 304
EcoT38I GRGCYC 1 cut(s) 289
ErhI CCWWGG 1 cut(s) 304
FaeI CATG 4 cut(s) 61, 285, 318, 425
FaiI YATR 7 cut(s) 59, 176, 197, 283, 316, 423, 447
FatI CATG 4 cut(s) 57, 281, 314, 421
FauI CCCGC 1 cut(s) 418
FbaI TGATCA 1 cut(s) 54
Fnu4HI GCNGC 6 cut(s) 30, 255, 258, 416, 439, 480
FokI GGATG 1 cut(s) 360
FriOI GRGCYC 1 cut(s) 289
Fsp4HI GCNGC 6 cut(s) 30, 255, 258, 416, 439, 480
FspBI CTAG 4 cut(s) 84, 129, 251, 528
GluI GCNGC 6 cut(s) 30, 255, 258, 416, 439, 480
GsuI CTGGAG 2 cut(s) 204, 531
HaeIII GGCC 4 cut(s) 114, 212, 302, 450
HapII CCGG 4 cut(s) 213, 452, 476, 483
HgaI GACGC 1 cut(s) 513
Hin1II CATG 4 cut(s) 61, 285, 318, 425
HpaII CCGG 4 cut(s) 213, 452, 476, 483
HphI GGTGA 1 cut(s) 116
Hpy188I TCNGA 4 cut(s) 9, 48, 230, 492
Hpy188III TCNNGA 1 cut(s) 385
HpyAV CCTTC 1 cut(s) 12
HpyCH4III ACNGT 3 cut(s) 13, 391, 461
HpyCH4IV ACGT 1 cut(s) 24
HpyCH4V TGCA 3 cut(s) 149, 257, 318
HpyF10VI GCNNNNNNNGC 3 cut(s) 278, 293, 447
HpyF3I CTNAG 3 cut(s) 6, 356, 396
HpySE526I ACGT 1 cut(s) 24
Hsp92II CATG 4 cut(s) 61, 285, 318, 425
KroI GCCGGC 1 cut(s) 482
KroNI GCCGGC 1 cut(s) 484
Ksp22I TGATCA 1 cut(s) 54
Kzo9I GATC 4 cut(s) 54, 66, 263, 487
LmnI GCTCC 2 cut(s) 37, 215
Lsp1109I GCAGC 6 cut(s) 41, 241, 269, 402, 450, 466
LweI GCATC 1 cut(s) 209
MaeI CTAG 4 cut(s) 84, 129, 251, 528
MaeII ACGT 1 cut(s) 24
MaeIII GTNAC 2 cut(s) 391, 461
MalI GATC 4 cut(s) 56, 68, 265, 489
MboI GATC 4 cut(s) 54, 66, 263, 487
MboII GAAGA 1 cut(s) 424
MhlI GDGCHC 2 cut(s) 289, 506
MlsI TGGCCA 1 cut(s) 114
MluNI TGGCCA 1 cut(s) 114
MnlI CCTC 9 cut(s) 131, 231, 329, 338, 345, 351, 361, 419, 506
Mox20I TGGCCA 1 cut(s) 114
MroNI GCCGGC 1 cut(s) 482
MscI TGGCCA 1 cut(s) 114
MseI TTAA 1 cut(s) 538
MslI CAYNNNNRTG 1 cut(s) 426
Msp20I TGGCCA 1 cut(s) 114
MspI CCGG 4 cut(s) 213, 452, 476, 483
MspR9I CCNGG 3 cut(s) 452, 453, 476
MwoI GCNNNNNNNGC 3 cut(s) 278, 293, 447
NaeI GCCGGC 1 cut(s) 484
NciI CCSGG 3 cut(s) 452, 453, 476
NdeII GATC 4 cut(s) 54, 66, 263, 487
NgoMIV GCCGGC 1 cut(s) 482
NlaIII CATG 4 cut(s) 61, 285, 318, 425
NmuCI GTSAC 2 cut(s) 391, 461
NspI RCATGY 1 cut(s) 318
PaeI GCATGC 1 cut(s) 318
PdiI GCCGGC 1 cut(s) 484
PkrI GCNGC 6 cut(s) 31, 256, 259, 417, 440, 481
PspPI GGNCC 2 cut(s) 301, 449
PstI CTGCAG 1 cut(s) 259
RsaI GTAC 1 cut(s) 407
RsaNI GTAC 1 cut(s) 406
RseI CAYNNNNRTG 1 cut(s) 426
SaqAI TTAA 1 cut(s) 538
SatI GCNGC 6 cut(s) 30, 255, 258, 416, 439, 480
Sau3AI GATC 4 cut(s) 54, 66, 263, 487
Sau96I GGNCC 2 cut(s) 301, 449
ScrFI CCNGG 3 cut(s) 452, 453, 476
SduI GDGCHC 2 cut(s) 289, 506
SetI ASST 8 cut(s) 27, 34, 104, 210, 235, 256, 402, 411
SfaNI GCATC 1 cut(s) 209
SfcI CTRYAG 1 cut(s) 255
SmaI CCCGGG 1 cut(s) 453
SmiMI CAYNNNNRTG 1 cut(s) 426
SphI GCATGC 1 cut(s) 318
SrfI GCCCGGGC 1 cut(s) 453
SsiI CCGC 1 cut(s) 425
SspMI CTAG 4 cut(s) 84, 129, 251, 528
StyD4I CCNGG 3 cut(s) 450, 451, 474
StyI CCWWGG 1 cut(s) 304
TaaI ACNGT 3 cut(s) 13, 391, 461
TaiI ACGT 1 cut(s) 27
Tru1I TTAA 1 cut(s) 538
Tru9I TTAA 1 cut(s) 538
TscAI CASTG 1 cut(s) 303
TseFI GTSAC 2 cut(s) 391, 461
TseI GCWGC 6 cut(s) 29, 254, 257, 415, 438, 479
Tsp45I GTSAC 2 cut(s) 391, 461
TspDTI ATGAA 2 cut(s) 94, 410
TspMI CCCGGG 1 cut(s) 451
TspRI CASTG 1 cut(s) 303
XceI RCATGY 1 cut(s) 318
XmaI CCCGGG 1 cut(s) 451
XspI CTAG 4 cut(s) 84, 129, 251, 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.