Rh4DG059500

AWPM-19-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0012496
Physical Location & Seq
Forward (+)
9042111 .. 9043514
1404 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG059500.1

Sequence Viewer

Length: 435 bp
ATGCGACGAAAAATTTGCACAGGTTTTGGAGGAAATGGTGCAACAATGTTCTTTCTGATCTTCTCCATATTGGCTGGTGTGATGGGAGTAGCGTCTAAGTTGGCTGGAGCAGGTCATCTCAGAGCTTGGAGGAGTGACAGTCTAGCTGCAGCCGGAGTTTCATCAATTGTGGCTTGGGCTCTCACTGTACTAGCCTTTGGATTGGCATGCAAGCAAATCCACATTGGAGGACACAGGGGATGGAGGCTGAAGGTTTTGGAGGCTTTTATAATAATCTTGACGTTCACCGAGCTTTTGTATCTCTTGCTACTTCATGCTGGTATCTGCAGCAGCAAGTATGGTCCGGGTTACCGCGACACCGACTACCCTGCTGCTCCCGGTGTCGATCCAATCCACAAGGGCGGAACTGGAGTCCCTGCTTCTAGGGTGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AWPM-19

Protein Analysis

144

Amino Acids

15.18

Weight (kDa)

9.88

Isoelectric Point (pI)

36.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AWPM-19 PF05512 9 - 107 8.5e-44 AWPM-19-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012496)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04560
fragaria_vesca FvH4_4g05050 FvH4_4g05050
malus_domestica MD13G1224200.v1.1 MD16G1229000.v1.1
prunus_persica Prupe.1G050900_v2.0.a1 Prupe.1G050900_v2.0.a1
pyrus_communis pycom13g19800
rosa_chinensis RchiOBHm_Chr4g0395971
rosa_laevigata RLG00000009584
rosa_multiflora Rmu_sc0001103.1_g000004
rosa_roxburghii Rroxscaffold_5G00340730
rosa_rugosa Rorug03G0353100
rosa_samantha Rh4AG064300 Rh4AG066300 Rh4BG063000 Rh4CG069400 Rh4DG059500
rosa_wichuraiana Rw4G005190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 269
Acc36I ACCTGC 1 cut(s) 101
AccII CGCG 1 cut(s) 354
AciI CCGC 2 cut(s) 352, 402
AclWI GGATC 1 cut(s) 380
AcsI RAATTY 1 cut(s) 12
AcuI CTGAAG 1 cut(s) 269
AfaI GTAC 1 cut(s) 189
AluBI AGCT 3 cut(s) 125, 146, 292
AluI AGCT 3 cut(s) 125, 146, 292
AlwI GGATC 1 cut(s) 380
ApeKI GCWGC 5 cut(s) 146, 149, 327, 330, 371
ApoI RAATTY 1 cut(s) 12
ArsI GACNNNNNNTTYG 1 cut(s) 29
AspS9I GGNCC 1 cut(s) 341
AsuC2I CCSGG 2 cut(s) 345, 378
AsuHPI GGTGA 1 cut(s) 277
AvaII GGWCC 1 cut(s) 341
BanII GRGCYC 1 cut(s) 181
BbvI GCAGC 5 cut(s) 133, 161, 339, 342, 358
BccI CCATC 2 cut(s) 76, 234
BcgI CGANNNNNNTGC 3 cut(s) 31, 350, 384
BcnI CCSGG 2 cut(s) 345, 378
BfaI CTAG 3 cut(s) 143, 191, 423
BfmI CTRYAG 2 cut(s) 147, 325
BfuAI ACCTGC 1 cut(s) 101
BisI GCNGC 5 cut(s) 147, 150, 328, 331, 372
BlsI GCNGC 5 cut(s) 148, 151, 329, 332, 373
Bme1390I CCNGG 2 cut(s) 345, 378
Bme18I GGWCC 1 cut(s) 341
BmgT120I GGNCC 1 cut(s) 341
BmrFI CCNGG 2 cut(s) 345, 378
BpmI CTGGAG 2 cut(s) 126, 429
BpuMI CCSGG 2 cut(s) 345, 378
BsaXI ACNNNNNCTCC 2 cut(s) 124, 154
Bse1I ACTGG 1 cut(s) 412
BseGI GGATG 1 cut(s) 245
BseMII CTCAG 1 cut(s) 133
BseNI ACTGG 1 cut(s) 412
BseRI GAGGAG 1 cut(s) 145
BseXI GCAGC 5 cut(s) 133, 161, 339, 342, 358
Bsh1236I CGCG 1 cut(s) 354
BsiSI CCGG 3 cut(s) 153, 344, 378
BslFI GGGAC 1 cut(s) 398
BsmFI GGGAC 1 cut(s) 398
Bsp1286I GDGCHC 1 cut(s) 181
Bsp143I GATC 2 cut(s) 57, 385
BspACI CCGC 2 cut(s) 352, 402
BspCNI CTCAG 1 cut(s) 132
BspFNI CGCG 1 cut(s) 354
BspMAI CTGCAG 2 cut(s) 151, 329
BspMI ACCTGC 1 cut(s) 101
BspPI GGATC 1 cut(s) 380
BsrI ACTGG 1 cut(s) 412
BssMI GATC 2 cut(s) 57, 385
Bst4CI ACNGT 2 cut(s) 140, 187
BstC8I GCNNGC 2 cut(s) 208, 212
BstDEI CTNAG 2 cut(s) 96, 119
BstEII GGTNACC 1 cut(s) 347
BstF5I GGATG 1 cut(s) 245
BstFNI CGCG 1 cut(s) 354
BstKTI GATC 2 cut(s) 60, 388
BstMBI GATC 2 cut(s) 57, 385
BstNSI RCATGY 1 cut(s) 210
BstPI GGTNACC 1 cut(s) 347
BstSCI CCNGG 2 cut(s) 343, 376
BstSFI CTRYAG 2 cut(s) 147, 325
BstUI CGCG 1 cut(s) 354
BstV1I GCAGC 5 cut(s) 133, 161, 339, 342, 358
BtsCI GGATG 1 cut(s) 245
BtsIMutI CAGTG 1 cut(s) 183
BveI ACCTGC 1 cut(s) 101
Cac8I GCNNGC 2 cut(s) 208, 212
Cfr13I GGNCC 1 cut(s) 341
CseI GACGC 1 cut(s) 81
Csp6I GTAC 1 cut(s) 188
CviAII CATG 2 cut(s) 207, 314
CviQI GTAC 1 cut(s) 188
DdeI CTNAG 2 cut(s) 96, 119
DpnI GATC 2 cut(s) 59, 387
DpnII GATC 2 cut(s) 57, 385
EciI GGCGGA 1 cut(s) 417
Eco24I GRGCYC 1 cut(s) 181
Eco47I GGWCC 1 cut(s) 341
Eco57I CTGAAG 1 cut(s) 269
Eco91I GGTNACC 1 cut(s) 347
EcoO65I GGTNACC 1 cut(s) 347
EcoT38I GRGCYC 1 cut(s) 181
FaeI CATG 2 cut(s) 210, 317
FaiI YATR 5 cut(s) 68, 208, 269, 315, 339
FaqI GGGAC 1 cut(s) 398
FatI CATG 2 cut(s) 206, 313
Fnu4HI GCNGC 5 cut(s) 147, 150, 328, 331, 372
FokI GGATG 1 cut(s) 252
FriOI GRGCYC 1 cut(s) 181
Fsp4HI GCNGC 5 cut(s) 147, 150, 328, 331, 372
FspBI CTAG 3 cut(s) 143, 191, 423
GluI GCNGC 5 cut(s) 147, 150, 328, 331, 372
GsuI CTGGAG 2 cut(s) 126, 429
HapII CCGG 3 cut(s) 153, 344, 378
HgaI GACGC 1 cut(s) 81
Hin1II CATG 2 cut(s) 210, 317
HinfI GANTC 1 cut(s) 411
HpaII CCGG 3 cut(s) 153, 344, 378
HphI GGTGA 1 cut(s) 277
Hpy166II GTNNAC 1 cut(s) 285
Hpy188I TCNGA 2 cut(s) 57, 122
Hpy188III TCNNGA 1 cut(s) 277
Hpy8I GTNNAC 1 cut(s) 285
Hpy99I CGWCG 1 cut(s) 9
HpyAV CCTTC 1 cut(s) 244
HpyCH4III ACNGT 2 cut(s) 140, 187
HpyCH4IV ACGT 1 cut(s) 281
HpyCH4V TGCA 5 cut(s) 18, 41, 149, 210, 327
HpyF3I CTNAG 2 cut(s) 96, 119
HpySE526I ACGT 1 cut(s) 281
Hsp92II CATG 2 cut(s) 210, 317
Kzo9I GATC 2 cut(s) 57, 385
LmnI GCTCC 2 cut(s) 107, 379
Lsp1109I GCAGC 5 cut(s) 133, 161, 339, 342, 358
MaeI CTAG 3 cut(s) 143, 191, 423
MaeII ACGT 1 cut(s) 281
MaeIII GTNAC 2 cut(s) 134, 347
MalI GATC 2 cut(s) 59, 387
MboI GATC 2 cut(s) 57, 385
MboII GAAGA 1 cut(s) 52
MfeI CAATTG 1 cut(s) 165
MhlI GDGCHC 1 cut(s) 181
MluCI AATT 2 cut(s) 12, 165
MlyI GAGTC 1 cut(s) 420
MnlI CCTC 5 cut(s) 23, 123, 221, 237, 253
MspI CCGG 3 cut(s) 153, 344, 378
MspR9I CCNGG 2 cut(s) 345, 378
MunI CAATTG 1 cut(s) 165
MvnI CGCG 1 cut(s) 354
NciI CCSGG 2 cut(s) 345, 378
NdeII GATC 2 cut(s) 57, 385
NlaIII CATG 2 cut(s) 210, 317
NmuCI GTSAC 1 cut(s) 134
NspI RCATGY 1 cut(s) 210
PaeI GCATGC 1 cut(s) 210
PkrI GCNGC 5 cut(s) 148, 151, 329, 332, 373
PleI GAGTC 1 cut(s) 419
PpsI GAGTC 1 cut(s) 419
PsiI TTATAA 1 cut(s) 269
PspEI GGTNACC 1 cut(s) 347
PspPI GGNCC 1 cut(s) 341
PstI CTGCAG 2 cut(s) 151, 329
RsaI GTAC 1 cut(s) 189
RsaNI GTAC 1 cut(s) 188
SatI GCNGC 5 cut(s) 147, 150, 328, 331, 372
Sau3AI GATC 2 cut(s) 57, 385
Sau96I GGNCC 1 cut(s) 341
SchI GAGTC 1 cut(s) 420
ScrFI CCNGG 2 cut(s) 345, 378
SduI GDGCHC 1 cut(s) 181
SetI ASST 7 cut(s) 25, 115, 127, 148, 255, 284, 294
SfcI CTRYAG 2 cut(s) 147, 325
SinI GGWCC 1 cut(s) 341
SphI GCATGC 1 cut(s) 210
Sse9I AATT 2 cut(s) 12, 165
SsiI CCGC 2 cut(s) 352, 402
SspMI CTAG 3 cut(s) 143, 191, 423
StyD4I CCNGG 2 cut(s) 343, 376
TaaI ACNGT 2 cut(s) 140, 187
TaiI ACGT 1 cut(s) 284
TaqI TCGA 1 cut(s) 384
TasI AATT 2 cut(s) 12, 165
TatI WGTACW 1 cut(s) 187
TscAI CASTG 1 cut(s) 190
TseFI GTSAC 1 cut(s) 134
TseI GCWGC 5 cut(s) 146, 149, 327, 330, 371
Tsp45I GTSAC 1 cut(s) 134
TspDTI ATGAA 2 cut(s) 150, 302
TspRI CASTG 1 cut(s) 190
VpaK11BI GGWCC 1 cut(s) 341
XapI RAATTY 1 cut(s) 12
XceI RCATGY 1 cut(s) 210
XspI CTAG 3 cut(s) 143, 191, 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.