MD13G1224200.v1.1

AWPM-19-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0012496
Physical Location & Seq
Forward (+)
21891290 .. 21892806
1517 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1224200.v1.1.491

Sequence Viewer

Length: 543 bp
ATGGCTCAGACTGTGGGAAGGAACGTGGCAGCTCCATTGCTGTTTCTCAACTTGATCATGTATTTGATCGTTGTCGGTTTTGCTAGTTGGTGTCTCAACAAGTTCATCAATGGCCAGACTAACCACCCTAGTTTTGGAGGAAATGGTGCGACTATGTTCTTTTTGGTATTCTCCATATTGGCTGGAGTGATGGGCACAGCATCAAAGCTGGCCGGAGCAAACCACATCAGATCTTGGAGGAGTGATAGCTTAGCTGCAGCAGGATCATCCGCCATCGTGGCATGGGCTCTCACTGCACTGGCCTTTGGGTTAGCATGCAAGGAAATAAACATTGGAGGATACAGAGGATGGAGACTGAAGGTCTTGGAGGCTTTCATAATAATCCTGACGTTCACTCAGCTCTTGTACCTCTTGATGCTTCATGCGGGCGTCTTCAGCAGCAAGTACGGCCCGGGTTACCGTGACACCGACTACCCAGTTACTGGCCGGGATCCGATACACAAGGGCACCACCGGAGGAGTCCCTGCTTCTAGGGTTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AWPM-19

Protein Analysis

181

Amino Acids

19.26

Weight (kDa)

9.88

Isoelectric Point (pI)

30.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AWPM-19 PF05512 15 - 143 2.2e-55 AWPM-19-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012496)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04560
fragaria_vesca FvH4_4g05050 FvH4_4g05050
malus_domestica MD13G1224200.v1.1 MD16G1229000.v1.1
prunus_persica Prupe.1G050900_v2.0.a1 Prupe.1G050900_v2.0.a1
pyrus_communis pycom13g19800
rosa_chinensis RchiOBHm_Chr4g0395971
rosa_laevigata RLG00000009584
rosa_multiflora Rmu_sc0001103.1_g000004
rosa_roxburghii Rroxscaffold_5G00340730
rosa_rugosa Rorug03G0353100
rosa_samantha Rh4AG064300 Rh4AG066300 Rh4BG063000 Rh4CG069400 Rh4DG059500
rosa_wichuraiana Rw4G005190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 506
AccB7I CCANNNNNTGG 1 cut(s) 482
AciI CCGC 2 cut(s) 270, 425
AclWI GGATC 3 cut(s) 271, 485, 498
AcoI YGGCCR 3 cut(s) 112, 210, 484
AcuI CTGAAG 2 cut(s) 377, 418
AcyI GRCGYC 1 cut(s) 429
AfaI GTAC 2 cut(s) 407, 446
AfiI CCNNNNNNNGG 2 cut(s) 134, 482
AhdI GACNNNNNGTC 1 cut(s) 359
AjuI GAANNNNNNNTTGG 2 cut(s) 315, 347
AluBI AGCT 5 cut(s) 32, 208, 249, 254, 400
AluI AGCT 5 cut(s) 32, 208, 249, 254, 400
Alw26I GTCTC 2 cut(s) 98, 346
AlwI GGATC 3 cut(s) 271, 485, 498
AlwNI CAGNNNCTG 1 cut(s) 482
Ama87I CYCGRG 1 cut(s) 451
AoxI GGCC 5 cut(s) 112, 210, 300, 448, 484
ApeKI GCWGC 4 cut(s) 29, 254, 257, 438
AspS9I GGNCC 1 cut(s) 449
AsuC2I CCSGG 3 cut(s) 452, 453, 488
AvaI CYCGRG 1 cut(s) 451
BaeGI GKGCMC 2 cut(s) 197, 509
BalI TGGCCA 1 cut(s) 114
BamHI GGATCC 1 cut(s) 490
BanI GGYRCC 1 cut(s) 506
BanII GRGCYC 1 cut(s) 289
BbsI GAAGAC 1 cut(s) 424
BbvI GCAGC 4 cut(s) 41, 241, 269, 450
BccI CCATC 3 cut(s) 184, 281, 342
BceAI ACGGC 1 cut(s) 463
BciVI GTATCC 1 cut(s) 332
BclI TGATCA 1 cut(s) 54
BcnI CCSGG 3 cut(s) 452, 453, 488
BcoDI GTCTC 2 cut(s) 98, 346
BfaI CTAG 3 cut(s) 84, 129, 531
BfmI CTRYAG 1 cut(s) 255
BfuI GTATCC 1 cut(s) 332
BglI GCCNNNNNGGC 1 cut(s) 278
BglII AGATCT 1 cut(s) 230
BisI GCNGC 4 cut(s) 30, 255, 258, 439
BlpI GCTNAGC 1 cut(s) 250
BlsI GCNGC 4 cut(s) 31, 256, 259, 440
Bme1390I CCNGG 3 cut(s) 452, 453, 488
BmeRI GACNNNNNGTC 1 cut(s) 359
BmeT110I CYCGRG 1 cut(s) 451
BmgT120I GGNCC 1 cut(s) 449
BmiI GGNNCC 2 cut(s) 492, 508
BmrFI CCNGG 3 cut(s) 452, 453, 488
BmrI ACTGGG 1 cut(s) 470
BmsI GCATC 2 cut(s) 209, 405
BmuI ACTGGG 1 cut(s) 470
BpiI GAAGAC 1 cut(s) 424
BpmI CTGGAG 1 cut(s) 204
Bpu1102I GCTNAGC 1 cut(s) 250
BpuMI CCSGG 3 cut(s) 452, 453, 488
BsaHI GRCGYC 1 cut(s) 429
BsaJI CCNNGG 1 cut(s) 451
BsaWI WCCGGW 1 cut(s) 512
Bsc4I CCNNNNNNNGG 2 cut(s) 134, 482
Bse1I ACTGG 3 cut(s) 303, 476, 487
Bse3DI GCAATG 1 cut(s) 35
BseDI CCNNGG 1 cut(s) 451
BseGI GGATG 2 cut(s) 266, 353
BseLI CCNNNNNNNGG 2 cut(s) 134, 482
BseMI GCAATG 1 cut(s) 35
BseMII CTCAG 2 cut(s) 20, 410
BseNI ACTGG 3 cut(s) 303, 476, 487
BseRI GAGGAG 2 cut(s) 253, 531
BseSI GKGCMC 2 cut(s) 197, 509
BseXI GCAGC 4 cut(s) 41, 241, 269, 450
BsgI GTGCAG 1 cut(s) 279
BshFI GGCC 5 cut(s) 114, 212, 302, 450, 486
BshNI GGYRCC 1 cut(s) 506
BsiHKCI CYCGRG 1 cut(s) 451
BsiSI CCGG 4 cut(s) 213, 452, 487, 513
BslFI GGGAC 1 cut(s) 506
BslI CCNNNNNNNGG 2 cut(s) 134, 482
BsmAI GTCTC 2 cut(s) 98, 346
BsmFI GGGAC 1 cut(s) 506
BsnI GGCC 5 cut(s) 114, 212, 302, 450, 486
BsoBI CYCGRG 1 cut(s) 451
Bsp1286I GDGCHC 3 cut(s) 197, 289, 509
Bsp143I GATC 5 cut(s) 54, 66, 230, 263, 490
Bsp1720I GCTNAGC 1 cut(s) 250
BspACI CCGC 2 cut(s) 270, 425
BspANI GGCC 5 cut(s) 114, 212, 302, 450, 486
BspCNI CTCAG 2 cut(s) 19, 409
BspLI GGNNCC 2 cut(s) 492, 508
BspMAI CTGCAG 1 cut(s) 259
BspPI GGATC 3 cut(s) 271, 485, 498
BspT107I GGYRCC 1 cut(s) 506
BsrDI GCAATG 1 cut(s) 35
BsrI ACTGG 3 cut(s) 303, 476, 487
BssECI CCNNGG 1 cut(s) 451
BssMI GATC 5 cut(s) 54, 66, 230, 263, 490
BssNI GRCGYC 1 cut(s) 429
Bst4CI ACNGT 2 cut(s) 13, 461
BstACI GRCGYC 1 cut(s) 429
BstC8I GCNNGC 3 cut(s) 210, 316, 427
BstDEI CTNAG 3 cut(s) 6, 250, 396
BstEII GGTNACC 1 cut(s) 455
BstF5I GGATG 2 cut(s) 266, 353
BstKTI GATC 5 cut(s) 57, 69, 233, 266, 493
BstMAI GTCTC 2 cut(s) 98, 346
BstMBI GATC 5 cut(s) 54, 66, 230, 263, 490
BstMWI GCNNNNNNNGC 4 cut(s) 278, 293, 435, 447
BstNSI RCATGY 1 cut(s) 318
BstPI GGTNACC 1 cut(s) 455
BstSCI CCNGG 3 cut(s) 450, 451, 486
BstSFI CTRYAG 1 cut(s) 255
BstSLI GKGCMC 2 cut(s) 197, 509
BstV1I GCAGC 4 cut(s) 41, 241, 269, 450
BstV2I GAAGAC 1 cut(s) 424
BstX2I RGATCY 2 cut(s) 230, 490
BstYI RGATCY 2 cut(s) 230, 490
BsuI GTATCC 1 cut(s) 332
BsuRI GGCC 5 cut(s) 114, 212, 302, 450, 486
BtsCI GGATG 2 cut(s) 266, 353
BtsI GCAGTG 1 cut(s) 291
BtsIMutI CAGTG 2 cut(s) 291, 296
Cac8I GCNNGC 3 cut(s) 210, 316, 427
CaiI CAGNNNCTG 1 cut(s) 482
Cfr13I GGNCC 1 cut(s) 449
Cfr9I CCCGGG 1 cut(s) 451
CseI GACGC 1 cut(s) 418
Csp6I GTAC 2 cut(s) 406, 445
CviAII CATG 4 cut(s) 58, 282, 315, 422
CviQI GTAC 2 cut(s) 406, 445
DdeI CTNAG 3 cut(s) 6, 250, 396
DpnI GATC 5 cut(s) 56, 68, 232, 265, 492
DpnII GATC 5 cut(s) 54, 66, 230, 263, 490
DriI GACNNNNNGTC 1 cut(s) 359
EaeI YGGCCR 3 cut(s) 112, 210, 484
Eam1105I GACNNNNNGTC 1 cut(s) 359
EciI GGCGGA 1 cut(s) 259
Eco24I GRGCYC 1 cut(s) 289
Eco57I CTGAAG 2 cut(s) 377, 418
Eco88I CYCGRG 1 cut(s) 451
Eco91I GGTNACC 1 cut(s) 455
EcoO65I GGTNACC 1 cut(s) 455
EcoT38I GRGCYC 1 cut(s) 289
FaeI CATG 4 cut(s) 61, 285, 318, 425
FaiI YATR 7 cut(s) 59, 155, 176, 283, 316, 377, 423
FaqI GGGAC 1 cut(s) 506
FatI CATG 4 cut(s) 57, 281, 314, 421
FauI CCCGC 1 cut(s) 418
FbaI TGATCA 1 cut(s) 54
Fnu4HI GCNGC 4 cut(s) 30, 255, 258, 439
FokI GGATG 2 cut(s) 253, 360
FriOI GRGCYC 1 cut(s) 289
Fsp4HI GCNGC 4 cut(s) 30, 255, 258, 439
FspBI CTAG 3 cut(s) 84, 129, 531
GluI GCNGC 4 cut(s) 30, 255, 258, 439
GsuI CTGGAG 1 cut(s) 204
HaeIII GGCC 5 cut(s) 114, 212, 302, 450, 486
HapII CCGG 4 cut(s) 213, 452, 487, 513
HgaI GACGC 1 cut(s) 418
Hin1I GRCGYC 1 cut(s) 429
Hin1II CATG 4 cut(s) 61, 285, 318, 425
HinfI GANTC 1 cut(s) 519
HpaII CCGG 4 cut(s) 213, 452, 487, 513
Hpy166II GTNNAC 1 cut(s) 393
Hpy188I TCNGA 3 cut(s) 9, 230, 495
Hpy188III TCNNGA 2 cut(s) 385, 412
Hpy8I GTNNAC 1 cut(s) 393
HpyAV CCTTC 2 cut(s) 12, 352
HpyCH4III ACNGT 2 cut(s) 13, 461
HpyCH4IV ACGT 2 cut(s) 24, 389
HpyCH4V TGCA 3 cut(s) 257, 296, 318
HpyF10VI GCNNNNNNNGC 4 cut(s) 278, 293, 435, 447
HpyF3I CTNAG 3 cut(s) 6, 250, 396
HpySE526I ACGT 2 cut(s) 24, 389
Hsp92I GRCGYC 1 cut(s) 429
Hsp92II CATG 4 cut(s) 61, 285, 318, 425
Ksp22I TGATCA 1 cut(s) 54
Kzo9I GATC 5 cut(s) 54, 66, 230, 263, 490
LmnI GCTCC 2 cut(s) 37, 215
Lsp1109I GCAGC 4 cut(s) 41, 241, 269, 450
LweI GCATC 2 cut(s) 209, 405
MaeI CTAG 3 cut(s) 84, 129, 531
MaeII ACGT 2 cut(s) 24, 389
MaeIII GTNAC 3 cut(s) 455, 461, 478
MalI GATC 5 cut(s) 56, 68, 232, 265, 492
MboI GATC 5 cut(s) 54, 66, 230, 263, 490
MboII GAAGA 1 cut(s) 424
MflI RGATCY 2 cut(s) 230, 490
MhlI GDGCHC 3 cut(s) 197, 289, 509
MlsI TGGCCA 1 cut(s) 114
MluNI TGGCCA 1 cut(s) 114
MlyI GAGTC 1 cut(s) 528
MnlI CCTC 7 cut(s) 131, 231, 329, 338, 361, 419, 509
Mox20I TGGCCA 1 cut(s) 114
MscI TGGCCA 1 cut(s) 114
MseI TTAA 1 cut(s) 541
Msp20I TGGCCA 1 cut(s) 114
MspI CCGG 4 cut(s) 213, 452, 487, 513
MspR9I CCNGG 3 cut(s) 452, 453, 488
MwoI GCNNNNNNNGC 4 cut(s) 278, 293, 435, 447
NciI CCSGG 3 cut(s) 452, 453, 488
NdeII GATC 5 cut(s) 54, 66, 230, 263, 490
NlaIII CATG 4 cut(s) 61, 285, 318, 425
NlaIV GGNNCC 2 cut(s) 492, 508
NmuCI GTSAC 1 cut(s) 461
NspI RCATGY 1 cut(s) 318
PaeI GCATGC 1 cut(s) 318
PflMI CCANNNNNTGG 1 cut(s) 482
PkrI GCNGC 4 cut(s) 31, 256, 259, 440
PleI GAGTC 1 cut(s) 527
PpsI GAGTC 1 cut(s) 527
PspEI GGTNACC 1 cut(s) 455
PspN4I GGNNCC 2 cut(s) 492, 508
PspPI GGNCC 1 cut(s) 449
PstI CTGCAG 1 cut(s) 259
PstNI CAGNNNCTG 1 cut(s) 482
PsuI RGATCY 2 cut(s) 230, 490
RsaI GTAC 2 cut(s) 407, 446
RsaNI GTAC 2 cut(s) 406, 445
SaqAI TTAA 1 cut(s) 541
SatI GCNGC 4 cut(s) 30, 255, 258, 439
Sau3AI GATC 5 cut(s) 54, 66, 230, 263, 490
Sau96I GGNCC 1 cut(s) 449
SchI GAGTC 1 cut(s) 528
ScrFI CCNGG 3 cut(s) 452, 453, 488
SduI GDGCHC 3 cut(s) 197, 289, 509
SetI ASST 9 cut(s) 27, 34, 210, 251, 256, 363, 392, 402, 411
SfaNI GCATC 2 cut(s) 209, 405
SfcI CTRYAG 1 cut(s) 255
SmaI CCCGGG 1 cut(s) 453
SphI GCATGC 1 cut(s) 318
SsiI CCGC 2 cut(s) 270, 425
SspMI CTAG 3 cut(s) 84, 129, 531
StyD4I CCNGG 3 cut(s) 450, 451, 486
TaaI ACNGT 2 cut(s) 13, 461
TaiI ACGT 2 cut(s) 27, 392
Tru1I TTAA 1 cut(s) 541
Tru9I TTAA 1 cut(s) 541
TscAI CASTG 2 cut(s) 298, 303
TseFI GTSAC 1 cut(s) 461
TseI GCWGC 4 cut(s) 29, 254, 257, 438
Tsp45I GTSAC 1 cut(s) 461
TspDTI ATGAA 3 cut(s) 94, 364, 410
TspMI CCCGGG 1 cut(s) 451
TspRI CASTG 2 cut(s) 298, 303
Van91I CCANNNNNTGG 1 cut(s) 482
XceI RCATGY 1 cut(s) 318
XcmI CCANNNNNNNNNTGG 1 cut(s) 131
XmaI CCCGGG 1 cut(s) 451
XspI CTAG 3 cut(s) 84, 129, 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.