FvH4_4g05510

Hydroxyethylthiazole

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0013360
Physical Location & Seq
Reverse (-)
4770288 .. 4773546
3259 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g05510.t2

Sequence Viewer

Length: 828 bp
ATGGAAGAATCAGAGGCAACCCTCTGGGCCAGGAAGTCGTGGGACCTACTGTGCGCCGTCCGATCCCAGTCGCCGCTGATCCAGTGCATCACAAACTTCGTGTCAATGGATCTCATGGCCAACACTCTCTTGTCCGCCGGTGCATCACCGGCCATGCTCCACAGCCTCGAAGAAATCCCTGACTTCACGCCTCAGGTCCACGCGCTCTGCCTCAACGTCGGCACACTCTCCGCCGACTGGTTACCGGCCATGAAGCTCTCCGCCCAACTGGCAACCAAGTCCGGTAAGCCTTGGGTCCTCGACCCCGTCGCCGCCGGCGCTTCCGGTTTCCGGTTGAAGGCTTGTTTGGAACTTGTCGAGCTTAAGCCCACTGTTATACGAGGAAATGCGTCCGAGATTATCGCCCTCTCCAACGCCACCGTGGGACCCACTAAGGGTGTGGACAGTTCCCATGAGTCCACGGATGCAGTAGAATCGGCCAAGTCATTGGCTCGAGCCAGCGGGGCTATAGTTGCAGTTTCTGGAGCTGTGGATATTGTCACAGATGGGGAGAGGGTTGTTGGGGCGCATAATGGGGTTGCCATGATGACAAAGATTACGGCAACAGGGTGTTCTGTGACTGCTCTTGTTGCCGCCTTTGTTGCTGTTGAGAAAACACATGTACTGGAAGCTACAGCTGCGGCATTATCCCTATTTGGGATAGCGGGAGAGATAGGAATGGAAATGGCGAATGGTCCAGCAACCTTGCGGATTCACTTGATAGATTCACTCCACAGACTGGATGAAGCGTGTGTGCTTTCTCGTGTTAAAATCACCAGCTTGTCGTGA

Protein Analysis

276

Amino Acids

28.58

Weight (kDa)

5.49

Isoelectric Point (pI)

25.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HK PF02110 19 - 261 1.4e-71 Hydroxyethylthiazole kinase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013360)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24030
fragaria_vesca FvH4_4g05510 FvH4_4g05510
malus_domestica MD13G1229700.v1.1
prunus_persica Prupe.1G056900_v2.0.a1
pyrus_communis pycom13g20240
rosa_chinensis RchiOBHm_Chr4g0396631
rosa_laevigata RLG00000009514
rosa_multiflora Rmu_sc0006586.1_g000020 Rmu_sc0010969.1_g000006
rosa_roxburghii Rroxscaffold_2G00122890 Rroxscaffold_5G00341480
rosa_rugosa Rorug03G0348300
rosa_samantha Rh4AG070800 Rh4BG068800 Rh4CG076600 Rh4DG066000
rosa_wichuraiana Rw4G005660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 778
AccII CGCG 1 cut(s) 203
AclWI GGATC 3 cut(s) 57, 73, 117
AcoI YGGCCR 4 cut(s) 117, 150, 246, 477
AfaI GTAC 1 cut(s) 663
AfiI CCNNNNNNNGG 6 cut(s) 237, 330, 337, 434, 696, 778
AflII CTTAAG 1 cut(s) 362
AflIII ACRYGT 1 cut(s) 658
AgsI TTSAA 1 cut(s) 337
AjnI CCWGG 1 cut(s) 29
AjuI GAANNNNNNNTTGG 2 cut(s) 329, 361
AluBI AGCT 6 cut(s) 256, 361, 527, 671, 677, 819
AluI AGCT 6 cut(s) 256, 361, 527, 671, 677, 819
AlwI GGATC 3 cut(s) 57, 73, 117
AlwNI CAGNNNCTG 1 cut(s) 521
Ama87I CYCGRG 1 cut(s) 492
AoxI GGCC 5 cut(s) 27, 117, 150, 246, 477
ApeKI GCWGC 1 cut(s) 677
ArsI GACNNNNNNTTYG 2 cut(s) 86, 118
AspLEI GCGC 4 cut(s) 56, 205, 320, 568
AspS9I GGNCC 6 cut(s) 27, 43, 196, 295, 425, 734
AsuHPI GGTGA 2 cut(s) 138, 805
AvaI CYCGRG 1 cut(s) 492
AvaII GGWCC 5 cut(s) 43, 196, 295, 425, 734
AxyI CCTNAGG 1 cut(s) 192
BalI TGGCCA 1 cut(s) 119
BauI CACGAG 1 cut(s) 801
BbvI GCAGC 1 cut(s) 664
BccI CCATC 1 cut(s) 539
BceAI ACGGC 2 cut(s) 41, 615
BcgI CGANNNNNNTGC 2 cut(s) 456, 490
BciT130I CCWGG 1 cut(s) 31
BfmI CTRYAG 2 cut(s) 507, 672
BfoI RGCGCY 1 cut(s) 321
BfrI CTTAAG 1 cut(s) 362
BglI GCCNNNNNGGC 2 cut(s) 269, 503
BisI GCNGC 5 cut(s) 74, 312, 633, 678, 681
BlsI GCNGC 5 cut(s) 75, 313, 634, 679, 682
Bme1390I CCNGG 1 cut(s) 31
Bme18I GGWCC 5 cut(s) 43, 196, 295, 425, 734
BmeT110I CYCGRG 1 cut(s) 492
BmgT120I GGNCC 6 cut(s) 27, 43, 196, 295, 425, 734
BmiI GGNNCC 4 cut(s) 44, 296, 426, 427
BmrFI CCNGG 1 cut(s) 31
BmrI ACTGGG 1 cut(s) 61
BmsI GCATC 3 cut(s) 96, 152, 454
BmuI ACTGGG 1 cut(s) 61
BpmI CTGGAG 1 cut(s) 543
BsaJI CCNNGG 3 cut(s) 290, 420, 459
BsaWI WCCGGW 3 cut(s) 281, 323, 330
Bsc4I CCNNNNNNNGG 6 cut(s) 237, 330, 337, 434, 696, 778
Bse118I RCCGGY 4 cut(s) 137, 148, 244, 314
Bse1I ACTGG 6 cut(s) 67, 82, 242, 273, 669, 783
Bse21I CCTNAGG 1 cut(s) 192
BseBI CCWGG 1 cut(s) 31
BseDI CCNNGG 3 cut(s) 290, 420, 459
BseGI GGATG 2 cut(s) 469, 787
BseLI CCNNNNNNNGG 6 cut(s) 237, 330, 337, 434, 696, 778
BseMII CTCAG 1 cut(s) 206
BseNI ACTGG 6 cut(s) 67, 82, 242, 273, 669, 783
BseXI GCAGC 1 cut(s) 664
Bsh1236I CGCG 1 cut(s) 203
BshFI GGCC 5 cut(s) 29, 119, 152, 248, 479
BsiHKCI CYCGRG 1 cut(s) 492
BsiSI CCGG 7 cut(s) 138, 149, 245, 282, 315, 324, 331
BslFI GGGAC 2 cut(s) 56, 438
BslI CCNNNNNNNGG 6 cut(s) 237, 330, 337, 434, 696, 778
BsmFI GGGAC 2 cut(s) 56, 438
BsnI GGCC 5 cut(s) 29, 119, 152, 248, 479
BsoBI CYCGRG 1 cut(s) 492
Bsp143I GATC 3 cut(s) 62, 78, 109
BspANI GGCC 5 cut(s) 29, 119, 152, 248, 479
BspCNI CTCAG 1 cut(s) 205
BspFNI CGCG 1 cut(s) 203
BspLI GGNNCC 4 cut(s) 44, 296, 426, 427
BspPI GGATC 3 cut(s) 57, 73, 117
BspTI CTTAAG 1 cut(s) 362
BsrFI RCCGGY 4 cut(s) 137, 148, 244, 314
BsrI ACTGG 6 cut(s) 67, 82, 242, 273, 669, 783
BssAI RCCGGY 4 cut(s) 137, 148, 244, 314
BssECI CCNNGG 3 cut(s) 290, 420, 459
BssMI GATC 3 cut(s) 62, 78, 109
BssSI CACGAG 1 cut(s) 801
BssT1I CCWWGG 1 cut(s) 290
Bst2BI CACGAG 1 cut(s) 801
Bst2UI CCWGG 1 cut(s) 31
Bst4CI ACNGT 4 cut(s) 51, 373, 421, 446
BstAFI CTTAAG 1 cut(s) 362
BstC8I GCNNGC 2 cut(s) 316, 499
BstDEI CTNAG 2 cut(s) 192, 432
BstDSI CCRYGG 2 cut(s) 420, 459
BstEII GGTNACC 1 cut(s) 240
BstF5I GGATG 2 cut(s) 469, 787
BstFNI CGCG 1 cut(s) 203
BstH2I RGCGCY 1 cut(s) 321
BstHHI GCGC 4 cut(s) 56, 205, 320, 568
BstKTI GATC 3 cut(s) 65, 81, 112
BstMBI GATC 3 cut(s) 62, 78, 109
BstMWI GCNNNNNNNGC 8 cut(s) 149, 269, 317, 503, 512, 629, 641, 677
BstNI CCWGG 1 cut(s) 31
BstNSI RCATGY 1 cut(s) 662
BstPI GGTNACC 1 cut(s) 240
BstSCI CCNGG 1 cut(s) 29
BstSFI CTRYAG 2 cut(s) 507, 672
BstUI CGCG 1 cut(s) 203
BstV1I GCAGC 1 cut(s) 664
BstX2I RGATCY 1 cut(s) 109
BstXI CCANNNNNNTGG 1 cut(s) 487
BstYI RGATCY 1 cut(s) 109
Bsu36I CCTNAGG 1 cut(s) 192
BsuRI GGCC 5 cut(s) 29, 119, 152, 248, 479
BtgI CCRYGG 2 cut(s) 420, 459
BtsCI GGATG 2 cut(s) 469, 787
BtsIMutI CAGTG 2 cut(s) 89, 369
Cac8I GCNNGC 2 cut(s) 316, 499
CaiI CAGNNNCTG 1 cut(s) 521
CfoI GCGC 4 cut(s) 56, 205, 320, 568
Cfr10I RCCGGY 4 cut(s) 137, 148, 244, 314
Cfr13I GGNCC 6 cut(s) 27, 43, 196, 295, 425, 734
CseI GACGC 1 cut(s) 378
Csp6I GTAC 1 cut(s) 662
CviAII CATG 6 cut(s) 115, 154, 250, 452, 583, 659
CviQI GTAC 1 cut(s) 662
DdeI CTNAG 2 cut(s) 192, 432
DpnI GATC 3 cut(s) 64, 80, 111
DpnII GATC 3 cut(s) 62, 78, 109
EaeI YGGCCR 4 cut(s) 117, 150, 246, 477
EciI GGCGGA 3 cut(s) 124, 220, 250
Eco130I CCWWGG 1 cut(s) 290
Eco47I GGWCC 5 cut(s) 43, 196, 295, 425, 734
Eco81I CCTNAGG 1 cut(s) 192
Eco88I CYCGRG 1 cut(s) 492
Eco91I GGTNACC 1 cut(s) 240
EcoO109I RGGNCCY 3 cut(s) 43, 295, 425
EcoO65I GGTNACC 1 cut(s) 240
EcoRII CCWGG 1 cut(s) 29
EcoT14I CCWWGG 1 cut(s) 290
ErhI CCWWGG 1 cut(s) 290
FaeI CATG 6 cut(s) 118, 157, 253, 455, 586, 662
FaiI YATR 9 cut(s) 116, 155, 251, 377, 453, 509, 570, 584, 660
FaqI GGGAC 2 cut(s) 56, 438
FatI CATG 6 cut(s) 114, 153, 249, 451, 582, 658
FauI CCCGC 2 cut(s) 494, 697
Fnu4HI GCNGC 5 cut(s) 74, 312, 633, 678, 681
FokI GGATG 2 cut(s) 476, 794
Fsp4HI GCNGC 5 cut(s) 74, 312, 633, 678, 681
GlaI GCGC 4 cut(s) 55, 204, 319, 567
GluI GCNGC 5 cut(s) 74, 312, 633, 678, 681
GsuI CTGGAG 1 cut(s) 543
HaeII RGCGCY 1 cut(s) 321
HaeIII GGCC 5 cut(s) 29, 119, 152, 248, 479
HapII CCGG 7 cut(s) 138, 149, 245, 282, 315, 324, 331
HgaI GACGC 1 cut(s) 378
HhaI GCGC 4 cut(s) 56, 205, 320, 568
Hin1II CATG 6 cut(s) 118, 157, 253, 455, 586, 662
Hin6I GCGC 4 cut(s) 54, 203, 318, 566
HinP1I GCGC 4 cut(s) 54, 203, 318, 566
HinfI GANTC 5 cut(s) 8, 455, 473, 751, 764
HpaII CCGG 7 cut(s) 138, 149, 245, 282, 315, 324, 331
HphI GGTGA 2 cut(s) 138, 805
Hpy166II GTNNAC 3 cut(s) 199, 442, 459
Hpy188I TCNGA 3 cut(s) 13, 62, 394
Hpy188III TCNNGA 2 cut(s) 522, 825
Hpy8I GTNNAC 3 cut(s) 199, 442, 459
Hpy99I CGWCG 2 cut(s) 221, 311
HpyAV CCTTC 1 cut(s) 331
HpyCH4III ACNGT 4 cut(s) 51, 373, 421, 446
HpyCH4IV ACGT 1 cut(s) 216
HpyCH4V TGCA 4 cut(s) 87, 143, 467, 515
HpyF10VI GCNNNNNNNGC 8 cut(s) 149, 269, 317, 503, 512, 629, 641, 677
HpyF3I CTNAG 2 cut(s) 192, 432
HpySE526I ACGT 1 cut(s) 216
Hsp92II CATG 6 cut(s) 118, 157, 253, 455, 586, 662
HspAI GCGC 4 cut(s) 54, 203, 318, 566
KflI GGGWCCC 1 cut(s) 425
KroI GCCGGC 1 cut(s) 314
KroNI GCCGGC 1 cut(s) 316
Kzo9I GATC 3 cut(s) 62, 78, 109
LmnI GCTCC 2 cut(s) 162, 524
Lsp1109I GCAGC 1 cut(s) 664
LweI GCATC 3 cut(s) 96, 152, 454
MaeII ACGT 1 cut(s) 216
MaeIII GTNAC 3 cut(s) 240, 538, 616
MalI GATC 3 cut(s) 64, 80, 111
MboI GATC 3 cut(s) 62, 78, 109
MboII GAAGA 2 cut(s) 17, 182
MflI RGATCY 1 cut(s) 109
MlsI TGGCCA 1 cut(s) 119
MluNI TGGCCA 1 cut(s) 119
MlyI GAGTC 1 cut(s) 464
MmeI TCCRAC 1 cut(s) 435
MnlI CCTC 9 cut(s) 7, 32, 176, 201, 221, 308, 374, 416, 546
Mox20I TGGCCA 1 cut(s) 119
MreI CGCCGGCG 1 cut(s) 314
MroNI GCCGGC 1 cut(s) 314
MscI TGGCCA 1 cut(s) 119
MseI TTAA 2 cut(s) 363, 807
Msp20I TGGCCA 1 cut(s) 119
MspA1I CMGCKG 3 cut(s) 76, 501, 677
MspCI CTTAAG 1 cut(s) 362
MspI CCGG 7 cut(s) 138, 149, 245, 282, 315, 324, 331
MspR9I CCNGG 1 cut(s) 31
MvaI CCWGG 1 cut(s) 31
MvnI CGCG 1 cut(s) 203
MwoI GCNNNNNNNGC 8 cut(s) 149, 269, 317, 503, 512, 629, 641, 677
NaeI GCCGGC 1 cut(s) 316
NdeII GATC 3 cut(s) 62, 78, 109
NgoMIV GCCGGC 1 cut(s) 314
NlaIII CATG 6 cut(s) 118, 157, 253, 455, 586, 662
NlaIV GGNNCC 4 cut(s) 44, 296, 426, 427
NmuCI GTSAC 2 cut(s) 538, 616
NspI RCATGY 1 cut(s) 662
PaeR7I CTCGAG 1 cut(s) 492
PciI ACATGT 1 cut(s) 658
PdiI GCCGGC 1 cut(s) 316
PfeI GAWTC 4 cut(s) 8, 473, 751, 764
PflFI GACNNNGTC 1 cut(s) 305
PflMI CCANNNNNTGG 1 cut(s) 778
PkrI GCNGC 5 cut(s) 75, 313, 634, 679, 682
PleI GAGTC 1 cut(s) 463
PpsI GAGTC 1 cut(s) 463
PpuMI RGGWCCY 3 cut(s) 43, 295, 425
PscI ACATGT 1 cut(s) 658
Psp5II RGGWCCY 3 cut(s) 43, 295, 425
Psp6I CCWGG 1 cut(s) 29
PspEI GGTNACC 1 cut(s) 240
PspGI CCWGG 1 cut(s) 29
PspN4I GGNNCC 4 cut(s) 44, 296, 426, 427
PspPI GGNCC 6 cut(s) 27, 43, 196, 295, 425, 734
PspPPI RGGWCCY 3 cut(s) 43, 295, 425
PspXI VCTCGAGB 1 cut(s) 492
PstNI CAGNNNCTG 1 cut(s) 521
PsuI RGATCY 1 cut(s) 109
PsyI GACNNNGTC 1 cut(s) 305
PvuII CAGCTG 1 cut(s) 677
RsaI GTAC 1 cut(s) 663
RsaNI GTAC 1 cut(s) 662
SaqAI TTAA 2 cut(s) 363, 807
SatI GCNGC 5 cut(s) 74, 312, 633, 678, 681
Sau3AI GATC 3 cut(s) 62, 78, 109
Sau96I GGNCC 6 cut(s) 27, 43, 196, 295, 425, 734
SchI GAGTC 1 cut(s) 464
ScrFI CCNGG 1 cut(s) 31
SfaNI GCATC 3 cut(s) 96, 152, 454
SfcI CTRYAG 2 cut(s) 507, 672
Sfr274I CTCGAG 1 cut(s) 492
SgrAI CRCCGGYG 2 cut(s) 137, 314
SinI GGWCC 5 cut(s) 43, 196, 295, 425, 734
SlaI CTCGAG 1 cut(s) 492
SmlI CTYRAG 2 cut(s) 362, 492
SmoI CTYRAG 2 cut(s) 362, 492
StyD4I CCNGG 1 cut(s) 29
StyI CCWWGG 1 cut(s) 290
TaaI ACNGT 4 cut(s) 51, 373, 421, 446
TaiI ACGT 1 cut(s) 219
TaqI TCGA 4 cut(s) 168, 300, 357, 493
TatI WGTACW 1 cut(s) 661
TauI GCSGC 4 cut(s) 76, 314, 635, 683
TfiI GAWTC 4 cut(s) 8, 473, 751, 764
Tru1I TTAA 2 cut(s) 363, 807
Tru9I TTAA 2 cut(s) 363, 807
TscAI CASTG 2 cut(s) 89, 376
TseFI GTSAC 2 cut(s) 538, 616
TseI GCWGC 1 cut(s) 677
Tsp45I GTSAC 2 cut(s) 538, 616
TspDTI ATGAA 2 cut(s) 266, 798
TspGWI ACGGA 1 cut(s) 476
TspRI CASTG 2 cut(s) 89, 376
Tth111I GACNNNGTC 1 cut(s) 305
Van91I CCANNNNNTGG 1 cut(s) 778
Vha464I CTTAAG 1 cut(s) 362
VpaK11BI GGWCC 5 cut(s) 43, 196, 295, 425, 734
XceI RCATGY 1 cut(s) 662
XcmI CCANNNNNNNNNTGG 2 cut(s) 418, 436
XhoI CTCGAG 1 cut(s) 492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.