Rh4AG070800

Hydroxyethylthiazole

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0013360
Physical Location & Seq
Reverse (-)
14601257 .. 14605605
4349 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG070800.1

Sequence Viewer

Length: 843 bp
ATGGAAAGCAACAAGGAAGAACAAGCAGAGGCAACCCTCTGGGCCCGGAAGTCGTGGGCCCTACTCTCCGCCGTCCGATCCCAGTCGCCGCTGATCCAATGCATCACAAACTTCGTGTCAATGGATCTGATGGCCAACACTCTCTTATCCGCCGGTGCATCACCGGCCATGCTCCACGCCCTCGAAGAAATCCCGGACTTCACGTCTCAGGTCCACGCGCTCTGCCTCAACGTTGGCACACTCTCCGCCGACTGGTTGCCGGCCATGAAGCTCGCCGCCCAACTGGCACGCGAGTCCGGTAAGCCCTGGGTTCTCGACCCAGTCGCCGCCGGCGCTTCCGGTTTCCGGTTGAAAGCTTGTTTGGAGCTCGTGGAGCTTAAGCCGACTGTTATACGAGGAAATGCGTCCGAGATTATCGCCCTCGCCAACGCCTCTGTGGGACCCACCAAGGGTGTAGACAGTTCCCATGAGTCCACGGATGCAGTGGAATCGGCAAAGTTATTGGCTCGAGCCAGCGGTGCCGTAGTTGCAGTTTCCGGAGCTGTGGACATTGTCACAGATGGGGAGAGCGTTGTTGGTGCTCATAATGGGGTTGCCATGATGACAAAGATTACGGCAACAGGGTGTTCTGTCACTGCTCTCATTGCTGCTTTTATTGCTGTTGATCAAACACATGTACTGGAAGCTACAGCTGCGGCGTTATCCTTATTTGGGATTGCGGGAGAGATGGGAATGGAAATGGCGAACGGTCCAGCAACCTTGCGGATCCACTTGATAGATTCACTCCACGGACTGGATGAAGCGTGTGTGCTTTCTCGTGTTAAGATCACCAGCTTGTCGTGA

Protein Analysis

280

Amino Acids

28.96

Weight (kDa)

5.12

Isoelectric Point (pI)

25.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HK PF02110 22 - 266 2.7e-73 Hydroxyethylthiazole kinase family
Phos_pyr_kin PF08543 91 - 233 9.2e-07 Phosphomethylpyrimidine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013360)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24030
fragaria_vesca FvH4_4g05510 FvH4_4g05510
malus_domestica MD13G1229700.v1.1
prunus_persica Prupe.1G056900_v2.0.a1
pyrus_communis pycom13g20240
rosa_chinensis RchiOBHm_Chr4g0396631
rosa_laevigata RLG00000009514
rosa_multiflora Rmu_sc0006586.1_g000020 Rmu_sc0010969.1_g000006
rosa_roxburghii Rroxscaffold_2G00122890 Rroxscaffold_5G00341480
rosa_rugosa Rorug03G0348300
rosa_samantha Rh4AG070800 Rh4BG068800 Rh4CG076600 Rh4DG066000
rosa_wichuraiana Rw4G005660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 518
AccB7I CCANNNNNTGG 1 cut(s) 793
AccI GTMKAC 1 cut(s) 456
AccII CGCG 2 cut(s) 218, 291
AccIII TCCGGA 1 cut(s) 536
AclI AACGTT 1 cut(s) 231
AclWI GGATC 5 cut(s) 72, 88, 132, 760, 773
AcoI YGGCCR 3 cut(s) 132, 165, 261
AfaI GTAC 1 cut(s) 678
AfiI CCNNNNNNNGG 5 cut(s) 252, 345, 449, 711, 793
AflII CTTAAG 1 cut(s) 377
AflIII ACRYGT 1 cut(s) 673
AgsI TTSAA 1 cut(s) 352
AhdI GACNNNNNGTC 1 cut(s) 202
AjiI CACGTC 1 cut(s) 204
AjnI CCWGG 1 cut(s) 305
AjuI GAANNNNNNNTTGG 2 cut(s) 344, 376
AluBI AGCT 8 cut(s) 271, 356, 367, 376, 542, 686, 692, 834
AluI AGCT 8 cut(s) 271, 356, 367, 376, 542, 686, 692, 834
Alw21I GWGCWC 2 cut(s) 369, 583
Alw26I GTCTC 1 cut(s) 210
AlwI GGATC 5 cut(s) 72, 88, 132, 760, 773
Ama87I CYCGRG 1 cut(s) 507
Aor13HI TCCGGA 1 cut(s) 536
AoxI GGCC 5 cut(s) 42, 57, 132, 165, 261
ApaI GGGCCC 2 cut(s) 46, 61
ApeKI GCWGC 2 cut(s) 647, 692
ArsI GACNNNNNNTTYG 2 cut(s) 101, 133
AspLEI GCGC 2 cut(s) 220, 335
AspS9I GGNCC 7 cut(s) 42, 43, 57, 58, 211, 440, 749
AsuC2I CCSGG 2 cut(s) 46, 194
AsuHPI GGTGA 2 cut(s) 153, 820
AvaI CYCGRG 1 cut(s) 507
AvaII GGWCC 3 cut(s) 211, 440, 749
BaeGI GKGCMC 2 cut(s) 46, 61
BalI TGGCCA 1 cut(s) 134
BamHI GGATCC 1 cut(s) 765
BanI GGYRCC 1 cut(s) 518
BanII GRGCYC 3 cut(s) 46, 61, 369
BauI CACGAG 2 cut(s) 368, 816
Bbv12I GWGCWC 2 cut(s) 369, 583
BbvI GCAGC 2 cut(s) 634, 679
BccI CCATC 3 cut(s) 124, 554, 721
BceAI ACGGC 3 cut(s) 56, 506, 630
BcgI CGANNNNNNTGC 2 cut(s) 471, 505
BciT130I CCWGG 1 cut(s) 307
BclI TGATCA 1 cut(s) 664
BcnI CCSGG 2 cut(s) 46, 194
BcoDI GTCTC 1 cut(s) 210
BfmI CTRYAG 1 cut(s) 687
BfoI RGCGCY 1 cut(s) 336
BfrI CTTAAG 1 cut(s) 377
BglI GCCNNNNNGGC 1 cut(s) 284
BisI GCNGC 6 cut(s) 89, 276, 327, 648, 693, 696
BlsI GCNGC 6 cut(s) 90, 277, 328, 649, 694, 697
Bme1390I CCNGG 3 cut(s) 46, 194, 307
Bme18I GGWCC 3 cut(s) 211, 440, 749
BmeRI GACNNNNNGTC 1 cut(s) 202
BmeT110I CYCGRG 1 cut(s) 507
BmgBI CACGTC 1 cut(s) 204
BmgT120I GGNCC 7 cut(s) 42, 43, 57, 58, 211, 440, 749
BmiI GGNNCC 6 cut(s) 44, 59, 441, 442, 520, 767
BmrFI CCNGG 3 cut(s) 46, 194, 307
BmrI ACTGGG 2 cut(s) 76, 314
BmsI GCATC 3 cut(s) 111, 167, 469
BmuI ACTGGG 2 cut(s) 76, 314
BpuMI CCSGG 2 cut(s) 46, 194
BsaJI CCNNGG 5 cut(s) 305, 306, 447, 474, 787
BsaWI WCCGGW 4 cut(s) 296, 338, 345, 536
Bsc4I CCNNNNNNNGG 5 cut(s) 252, 345, 449, 711, 793
Bse118I RCCGGY 4 cut(s) 152, 163, 259, 329
Bse1I ACTGG 6 cut(s) 82, 257, 288, 320, 684, 798
Bse3DI GCAATG 1 cut(s) 642
BseAI TCCGGA 1 cut(s) 536
BseBI CCWGG 1 cut(s) 307
BseDI CCNNGG 5 cut(s) 305, 306, 447, 474, 787
BseGI GGATG 2 cut(s) 484, 802
BseLI CCNNNNNNNGG 5 cut(s) 252, 345, 449, 711, 793
BseMI GCAATG 1 cut(s) 642
BseMII CTCAG 1 cut(s) 221
BseNI ACTGG 6 cut(s) 82, 257, 288, 320, 684, 798
BseSI GKGCMC 2 cut(s) 46, 61
BseXI GCAGC 2 cut(s) 634, 679
Bsh1236I CGCG 2 cut(s) 218, 291
BshFI GGCC 5 cut(s) 44, 59, 134, 167, 263
BshNI GGYRCC 1 cut(s) 518
BsiHKAI GWGCWC 2 cut(s) 369, 583
BsiHKCI CYCGRG 1 cut(s) 507
BslFI GGGAC 1 cut(s) 453
BslI CCNNNNNNNGG 5 cut(s) 252, 345, 449, 711, 793
BsmAI GTCTC 1 cut(s) 210
BsmBI CGTCTC 1 cut(s) 210
BsmFI GGGAC 1 cut(s) 453
BsnI GGCC 5 cut(s) 44, 59, 134, 167, 263
BsoBI CYCGRG 1 cut(s) 507
Bsp120I GGGCCC 2 cut(s) 42, 57
Bsp1286I GDGCHC 4 cut(s) 46, 61, 369, 583
Bsp13I TCCGGA 1 cut(s) 536
Bsp143I GATC 6 cut(s) 77, 93, 124, 664, 765, 825
BspANI GGCC 5 cut(s) 44, 59, 134, 167, 263
BspCNI CTCAG 1 cut(s) 220
BspEI TCCGGA 1 cut(s) 536
BspFNI CGCG 2 cut(s) 218, 291
BspLI GGNNCC 6 cut(s) 44, 59, 441, 442, 520, 767
BspPI GGATC 5 cut(s) 72, 88, 132, 760, 773
BspT107I GGYRCC 1 cut(s) 518
BspTI CTTAAG 1 cut(s) 377
BsrDI GCAATG 1 cut(s) 642
BsrFI RCCGGY 4 cut(s) 152, 163, 259, 329
BsrI ACTGG 6 cut(s) 82, 257, 288, 320, 684, 798
BssAI RCCGGY 4 cut(s) 152, 163, 259, 329
BssECI CCNNGG 5 cut(s) 305, 306, 447, 474, 787
BssMI GATC 6 cut(s) 77, 93, 124, 664, 765, 825
BssSI CACGAG 2 cut(s) 368, 816
BssT1I CCWWGG 1 cut(s) 447
Bst2BI CACGAG 2 cut(s) 368, 816
Bst2UI CCWGG 1 cut(s) 307
Bst4CI ACNGT 3 cut(s) 388, 461, 749
BstAFI CTTAAG 1 cut(s) 377
BstC8I GCNNGC 5 cut(s) 261, 273, 289, 331, 514
BstDEI CTNAG 1 cut(s) 207
BstDSI CCRYGG 2 cut(s) 474, 787
BstF5I GGATG 2 cut(s) 484, 802
BstFNI CGCG 2 cut(s) 218, 291
BstH2I RGCGCY 1 cut(s) 336
BstHHI GCGC 2 cut(s) 220, 335
BstKTI GATC 6 cut(s) 80, 96, 127, 667, 768, 828
BstMAI GTCTC 1 cut(s) 210
BstMBI GATC 6 cut(s) 77, 93, 124, 664, 765, 825
BstMWI GCNNNNNNNGC 9 cut(s) 164, 284, 332, 373, 518, 527, 644, 656, 692
BstNI CCWGG 1 cut(s) 307
BstNSI RCATGY 1 cut(s) 677
BstSCI CCNGG 3 cut(s) 44, 192, 305
BstSFI CTRYAG 1 cut(s) 687
BstSLI GKGCMC 2 cut(s) 46, 61
BstUI CGCG 2 cut(s) 218, 291
BstV1I GCAGC 2 cut(s) 634, 679
BstX2I RGATCY 2 cut(s) 124, 765
BstYI RGATCY 2 cut(s) 124, 765
BsuRI GGCC 5 cut(s) 44, 59, 134, 167, 263
BtgI CCRYGG 2 cut(s) 474, 787
BtrI CACGTC 1 cut(s) 204
BtsCI GGATG 2 cut(s) 484, 802
BtsI GCAGTG 2 cut(s) 489, 633
BtsIMutI CAGTG 2 cut(s) 489, 633
Cac8I GCNNGC 5 cut(s) 261, 273, 289, 331, 514
CfoI GCGC 2 cut(s) 220, 335
Cfr10I RCCGGY 4 cut(s) 152, 163, 259, 329
Cfr13I GGNCC 7 cut(s) 42, 43, 57, 58, 211, 440, 749
CseI GACGC 1 cut(s) 393
Csp6I GTAC 1 cut(s) 677
CviAII CATG 5 cut(s) 169, 265, 467, 598, 674
CviQI GTAC 1 cut(s) 677
DdeI CTNAG 1 cut(s) 207
DpnI GATC 6 cut(s) 79, 95, 126, 666, 767, 827
DpnII GATC 6 cut(s) 77, 93, 124, 664, 765, 825
DriI GACNNNNNGTC 1 cut(s) 202
EaeI YGGCCR 3 cut(s) 132, 165, 261
Eam1105I GACNNNNNGTC 1 cut(s) 202
EciI GGCGGA 3 cut(s) 58, 139, 235
Ecl136II GAGCTC 1 cut(s) 367
Eco130I CCWWGG 1 cut(s) 447
Eco24I GRGCYC 3 cut(s) 46, 61, 369
Eco47I GGWCC 3 cut(s) 211, 440, 749
Eco53kI GAGCTC 1 cut(s) 367
Eco88I CYCGRG 1 cut(s) 507
EcoICRI GAGCTC 1 cut(s) 367
EcoO109I RGGNCCY 2 cut(s) 58, 440
EcoRII CCWGG 1 cut(s) 305
EcoT14I CCWWGG 1 cut(s) 447
EcoT22I ATGCAT 1 cut(s) 104
EcoT38I GRGCYC 3 cut(s) 46, 61, 369
ErhI CCWWGG 1 cut(s) 447
Esp3I CGTCTC 1 cut(s) 210
FaeI CATG 5 cut(s) 172, 268, 470, 601, 677
FaiI YATR 7 cut(s) 170, 266, 392, 468, 585, 599, 675
FaqI GGGAC 1 cut(s) 453
FatI CATG 5 cut(s) 168, 264, 466, 597, 673
FauI CCCGC 1 cut(s) 712
FbaI TGATCA 1 cut(s) 664
FblI GTMKAC 1 cut(s) 456
Fnu4HI GCNGC 6 cut(s) 89, 276, 327, 648, 693, 696
FokI GGATG 2 cut(s) 491, 809
FriOI GRGCYC 3 cut(s) 46, 61, 369
Fsp4HI GCNGC 6 cut(s) 89, 276, 327, 648, 693, 696
GlaI GCGC 2 cut(s) 219, 334
GluI GCNGC 6 cut(s) 89, 276, 327, 648, 693, 696
HaeII RGCGCY 1 cut(s) 336
HaeIII GGCC 5 cut(s) 44, 59, 134, 167, 263
HgaI GACGC 1 cut(s) 393
HhaI GCGC 2 cut(s) 220, 335
Hin1II CATG 5 cut(s) 172, 268, 470, 601, 677
Hin6I GCGC 2 cut(s) 218, 333
HinP1I GCGC 2 cut(s) 218, 333
HindIII AAGCTT 1 cut(s) 354
HinfI GANTC 4 cut(s) 293, 470, 488, 779
HphI GGTGA 2 cut(s) 153, 820
Hpy166II GTNNAC 4 cut(s) 214, 457, 474, 547
Hpy188I TCNGA 3 cut(s) 77, 129, 409
Hpy188III TCNNGA 3 cut(s) 314, 537, 840
Hpy8I GTNNAC 4 cut(s) 214, 457, 474, 547
HpyCH4III ACNGT 3 cut(s) 388, 461, 749
HpyCH4IV ACGT 2 cut(s) 203, 231
HpyCH4V TGCA 4 cut(s) 102, 158, 482, 530
HpyF10VI GCNNNNNNNGC 9 cut(s) 164, 284, 332, 373, 518, 527, 644, 656, 692
HpyF3I CTNAG 1 cut(s) 207
HpySE526I ACGT 2 cut(s) 203, 231
Hsp92II CATG 5 cut(s) 172, 268, 470, 601, 677
HspAI GCGC 2 cut(s) 218, 333
KflI GGGWCCC 1 cut(s) 440
Kpn2I TCCGGA 1 cut(s) 536
KroI GCCGGC 2 cut(s) 259, 329
KroNI GCCGGC 2 cut(s) 261, 331
Ksp22I TGATCA 1 cut(s) 664
Kzo9I GATC 6 cut(s) 77, 93, 124, 664, 765, 825
LmnI GCTCC 4 cut(s) 177, 364, 373, 539
Lsp1109I GCAGC 2 cut(s) 634, 679
LweI GCATC 3 cut(s) 111, 167, 469
MaeII ACGT 2 cut(s) 203, 231
MaeIII GTNAC 2 cut(s) 553, 631
MalI GATC 6 cut(s) 79, 95, 126, 666, 767, 827
MboI GATC 6 cut(s) 77, 93, 124, 664, 765, 825
MboII GAAGA 2 cut(s) 29, 197
MflI RGATCY 2 cut(s) 124, 765
MhlI GDGCHC 4 cut(s) 46, 61, 369, 583
MlsI TGGCCA 1 cut(s) 134
MluNI TGGCCA 1 cut(s) 134
MlyI GAGTC 2 cut(s) 302, 479
MnlI CCTC 7 cut(s) 22, 47, 191, 236, 389, 431, 442
Mox20I TGGCCA 1 cut(s) 134
Mph1103I ATGCAT 1 cut(s) 104
MreI CGCCGGCG 1 cut(s) 329
MroI TCCGGA 1 cut(s) 536
MroNI GCCGGC 2 cut(s) 259, 329
MscI TGGCCA 1 cut(s) 134
MseI TTAA 2 cut(s) 378, 822
Msp20I TGGCCA 1 cut(s) 134
MspA1I CMGCKG 3 cut(s) 91, 516, 692
MspCI CTTAAG 1 cut(s) 377
MspR9I CCNGG 3 cut(s) 46, 194, 307
MvaI CCWGG 1 cut(s) 307
MvnI CGCG 2 cut(s) 218, 291
MwoI GCNNNNNNNGC 9 cut(s) 164, 284, 332, 373, 518, 527, 644, 656, 692
NaeI GCCGGC 2 cut(s) 261, 331
NciI CCSGG 2 cut(s) 46, 194
NdeII GATC 6 cut(s) 77, 93, 124, 664, 765, 825
NgoMIV GCCGGC 2 cut(s) 259, 329
NlaIII CATG 5 cut(s) 172, 268, 470, 601, 677
NlaIV GGNNCC 6 cut(s) 44, 59, 441, 442, 520, 767
NmuCI GTSAC 2 cut(s) 553, 631
NsiI ATGCAT 1 cut(s) 104
NspI RCATGY 1 cut(s) 677
PaeR7I CTCGAG 1 cut(s) 507
PasI CCCWGGG 1 cut(s) 306
PciI ACATGT 1 cut(s) 673
PdiI GCCGGC 2 cut(s) 261, 331
PfeI GAWTC 2 cut(s) 488, 779
PflFI GACNNNGTC 2 cut(s) 320, 551
PflMI CCANNNNNTGG 1 cut(s) 793
PfoI TCCNGGA 1 cut(s) 192
PkrI GCNGC 6 cut(s) 90, 277, 328, 649, 694, 697
PleI GAGTC 2 cut(s) 301, 478
PpsI GAGTC 2 cut(s) 301, 478
PpuMI RGGWCCY 1 cut(s) 440
PscI ACATGT 1 cut(s) 673
Psp124BI GAGCTC 1 cut(s) 369
Psp1406I AACGTT 1 cut(s) 231
Psp5II RGGWCCY 1 cut(s) 440
Psp6I CCWGG 1 cut(s) 305
PspGI CCWGG 1 cut(s) 305
PspN4I GGNNCC 6 cut(s) 44, 59, 441, 442, 520, 767
PspOMI GGGCCC 2 cut(s) 42, 57
PspPI GGNCC 7 cut(s) 42, 43, 57, 58, 211, 440, 749
PspPPI RGGWCCY 1 cut(s) 440
PspXI VCTCGAGB 1 cut(s) 507
PsuI RGATCY 2 cut(s) 124, 765
PsyI GACNNNGTC 2 cut(s) 320, 551
PvuII CAGCTG 1 cut(s) 692
RsaI GTAC 1 cut(s) 678
RsaNI GTAC 1 cut(s) 677
SacI GAGCTC 1 cut(s) 369
SaqAI TTAA 2 cut(s) 378, 822
SatI GCNGC 6 cut(s) 89, 276, 327, 648, 693, 696
Sau3AI GATC 6 cut(s) 77, 93, 124, 664, 765, 825
Sau96I GGNCC 7 cut(s) 42, 43, 57, 58, 211, 440, 749
SchI GAGTC 2 cut(s) 302, 479
ScrFI CCNGG 3 cut(s) 46, 194, 307
SduI GDGCHC 4 cut(s) 46, 61, 369, 583
SfaNI GCATC 3 cut(s) 111, 167, 469
SfcI CTRYAG 1 cut(s) 687
Sfr274I CTCGAG 1 cut(s) 507
SgrAI CRCCGGYG 2 cut(s) 152, 329
SinI GGWCC 3 cut(s) 211, 440, 749
SlaI CTCGAG 1 cut(s) 507
SmlI CTYRAG 2 cut(s) 377, 507
SmoI CTYRAG 2 cut(s) 377, 507
SstI GAGCTC 1 cut(s) 369
StyD4I CCNGG 3 cut(s) 44, 192, 305
StyI CCWWGG 1 cut(s) 447
TaaI ACNGT 3 cut(s) 388, 461, 749
TaiI ACGT 2 cut(s) 206, 234
TaqI TCGA 3 cut(s) 183, 315, 508
TatI WGTACW 1 cut(s) 676
TauI GCSGC 4 cut(s) 91, 278, 329, 698
TfiI GAWTC 2 cut(s) 488, 779
Tru1I TTAA 2 cut(s) 378, 822
Tru9I TTAA 2 cut(s) 378, 822
TscAI CASTG 2 cut(s) 489, 640
TseFI GTSAC 2 cut(s) 553, 631
TseI GCWGC 2 cut(s) 647, 692
Tsp45I GTSAC 2 cut(s) 553, 631
TspDTI ATGAA 2 cut(s) 281, 813
TspGWI ACGGA 2 cut(s) 491, 804
TspRI CASTG 2 cut(s) 489, 640
Tth111I GACNNNGTC 2 cut(s) 320, 551
Van91I CCANNNNNTGG 1 cut(s) 793
Vha464I CTTAAG 1 cut(s) 377
VpaK11BI GGWCC 3 cut(s) 211, 440, 749
XceI RCATGY 1 cut(s) 677
XcmI CCANNNNNNNNNTGG 2 cut(s) 433, 481
XhoI CTCGAG 1 cut(s) 507
XmiI GTMKAC 1 cut(s) 456
Zsp2I ATGCAT 1 cut(s) 104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.