Rw4G005660

Hydroxyethylthiazole

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0013360
Physical Location & Seq
Reverse (-)
11379044 .. 11380134
1091 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G005660.1

Sequence Viewer

Length: 768 bp
ATGGAAAGCAACAAGGAAGAACAAGCAGAGGCAACCCTCTGGGCCCGGAAGTCGTGGGCCCTACTCTCCGCCGTCCGATCCCAGTCGCCGCTGATCCAATGCATCACAAACTTCGTGTCAATGGATCTGATGGCCAACACTCTCTTATCCGCCGGTGCATCACCGGCCATGCTCCACGCCCTCGAAGAAATCCCGGACTTCACGCCTCAGGTCCACGCGCTCTGCCTCAACGTCGGCACACTCTCCGCCGACTGGTTGCCGGCCATGAAGCTCGCCGCCCAACTGGCACGCGAGTCCGGTAAGCCCTGGGTTCTCGACCCTCTTGTGGAGCTTAAGCCCACTGTTATACGAGGAAATGCGTCCGAGATTATCGCCCTCGCCAACGCCTCTGTGGGACCCACCAAGGGTGTAGACAGTTCCCATGAGTCCACGGATGCAGTGGAATCGGCAAAGTTATTGGCTCGAGCCAGCGGTGCCGTAGTTGCAGTTTCCGGAGCTGTGGACATTGTCACAGATGGGGAGAGGGTTGTTGGTGCTCATAATGGGGGTGTTCTGTTCACTGCTCTCATTGCTGCTTTTATTGCTGTTGATCAAACACATGTACTGGAAGCTACAGCTGTGGCGTTGTCCTTGTTTGGGATTGCGGGAGAGATGGGAATGGAAATGGCGAATGGTCCAGCGACCTTGAGGATTCACTTGATAGATTCACTTCACGGACTGGATGAAGGGCGTGTGCTTTCTCGTATTAAAATCACCAGCTTGTCGTGA

Protein Analysis

255

Amino Acids

26.66

Weight (kDa)

5.1

Isoelectric Point (pI)

23.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HK PF02110 22 - 108 8.6e-24 Hydroxyethylthiazole kinase family
HK PF02110 110 - 241 2.1e-24 Hydroxyethylthiazole kinase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013360)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24030
fragaria_vesca FvH4_4g05510 FvH4_4g05510
malus_domestica MD13G1229700.v1.1
prunus_persica Prupe.1G056900_v2.0.a1
pyrus_communis pycom13g20240
rosa_chinensis RchiOBHm_Chr4g0396631
rosa_laevigata RLG00000009514
rosa_multiflora Rmu_sc0006586.1_g000020 Rmu_sc0010969.1_g000006
rosa_roxburghii Rroxscaffold_2G00122890 Rroxscaffold_5G00341480
rosa_rugosa Rorug03G0348300
rosa_samantha Rh4AG070800 Rh4BG068800 Rh4CG076600 Rh4DG066000
rosa_wichuraiana Rw4G005660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 473
AccI GTMKAC 1 cut(s) 411
AccII CGCG 2 cut(s) 218, 291
AccIII TCCGGA 1 cut(s) 491
AciI CCGC 7 cut(s) 69, 89, 150, 246, 276, 471, 644
AclWI GGATC 3 cut(s) 72, 88, 132
AcoI YGGCCR 3 cut(s) 132, 165, 261
AfaI GTAC 1 cut(s) 603
AfiI CCNNNNNNNGG 4 cut(s) 252, 325, 404, 636
AflII CTTAAG 1 cut(s) 332
AflIII ACRYGT 1 cut(s) 598
AjnI CCWGG 1 cut(s) 305
AluBI AGCT 6 cut(s) 271, 331, 497, 611, 617, 759
AluI AGCT 6 cut(s) 271, 331, 497, 611, 617, 759
Alw21I GWGCWC 1 cut(s) 538
AlwI GGATC 3 cut(s) 72, 88, 132
Ama87I CYCGRG 1 cut(s) 462
Aor13HI TCCGGA 1 cut(s) 491
AoxI GGCC 5 cut(s) 42, 57, 132, 165, 261
ApaI GGGCCC 2 cut(s) 46, 61
ApeKI GCWGC 1 cut(s) 572
ArsI GACNNNNNNTTYG 2 cut(s) 101, 133
AspLEI GCGC 1 cut(s) 220
AspS9I GGNCC 7 cut(s) 42, 43, 57, 58, 211, 395, 674
AsuC2I CCSGG 2 cut(s) 46, 194
AsuHPI GGTGA 2 cut(s) 153, 745
AvaI CYCGRG 1 cut(s) 462
AvaII GGWCC 3 cut(s) 211, 395, 674
AxyI CCTNAGG 1 cut(s) 207
BaeGI GKGCMC 2 cut(s) 46, 61
BalI TGGCCA 1 cut(s) 134
BanI GGYRCC 1 cut(s) 473
BanII GRGCYC 2 cut(s) 46, 61
Bbv12I GWGCWC 1 cut(s) 538
BbvI GCAGC 1 cut(s) 559
BccI CCATC 3 cut(s) 124, 509, 646
BceAI ACGGC 2 cut(s) 56, 461
BcgI CGANNNNNNTGC 2 cut(s) 426, 460
BciT130I CCWGG 1 cut(s) 307
BclI TGATCA 1 cut(s) 589
BcnI CCSGG 2 cut(s) 46, 194
BfmI CTRYAG 1 cut(s) 612
BfrI CTTAAG 1 cut(s) 332
BglI GCCNNNNNGGC 1 cut(s) 284
BisI GCNGC 3 cut(s) 89, 276, 573
BlsI GCNGC 3 cut(s) 90, 277, 574
Bme1390I CCNGG 3 cut(s) 46, 194, 307
Bme18I GGWCC 3 cut(s) 211, 395, 674
BmeT110I CYCGRG 1 cut(s) 462
BmgT120I GGNCC 7 cut(s) 42, 43, 57, 58, 211, 395, 674
BmiI GGNNCC 5 cut(s) 44, 59, 396, 397, 475
BmrFI CCNGG 3 cut(s) 46, 194, 307
BmrI ACTGGG 1 cut(s) 76
BmsI GCATC 3 cut(s) 111, 167, 424
BmuI ACTGGG 1 cut(s) 76
BpuEI CTTGAG 1 cut(s) 706
BpuMI CCSGG 2 cut(s) 46, 194
BsaJI CCNNGG 4 cut(s) 305, 306, 402, 429
BsaWI WCCGGW 2 cut(s) 296, 491
Bsc4I CCNNNNNNNGG 4 cut(s) 252, 325, 404, 636
Bse118I RCCGGY 3 cut(s) 152, 163, 259
Bse1I ACTGG 5 cut(s) 82, 257, 288, 609, 723
Bse21I CCTNAGG 1 cut(s) 207
Bse3DI GCAATG 1 cut(s) 567
BseAI TCCGGA 1 cut(s) 491
BseBI CCWGG 1 cut(s) 307
BseDI CCNNGG 4 cut(s) 305, 306, 402, 429
BseGI GGATG 2 cut(s) 439, 727
BseLI CCNNNNNNNGG 4 cut(s) 252, 325, 404, 636
BseMI GCAATG 1 cut(s) 567
BseMII CTCAG 1 cut(s) 221
BseNI ACTGG 5 cut(s) 82, 257, 288, 609, 723
BseSI GKGCMC 2 cut(s) 46, 61
BseXI GCAGC 1 cut(s) 559
Bsh1236I CGCG 2 cut(s) 218, 291
BshFI GGCC 5 cut(s) 44, 59, 134, 167, 263
BshNI GGYRCC 1 cut(s) 473
BsiHKAI GWGCWC 1 cut(s) 538
BsiHKCI CYCGRG 1 cut(s) 462
BsiSI CCGG 7 cut(s) 46, 153, 164, 194, 260, 297, 492
BslFI GGGAC 1 cut(s) 408
BslI CCNNNNNNNGG 4 cut(s) 252, 325, 404, 636
BsmFI GGGAC 1 cut(s) 408
BsnI GGCC 5 cut(s) 44, 59, 134, 167, 263
BsoBI CYCGRG 1 cut(s) 462
Bsp120I GGGCCC 2 cut(s) 42, 57
Bsp1286I GDGCHC 3 cut(s) 46, 61, 538
Bsp13I TCCGGA 1 cut(s) 491
Bsp143I GATC 4 cut(s) 77, 93, 124, 589
BspACI CCGC 7 cut(s) 69, 89, 150, 246, 276, 471, 644
BspANI GGCC 5 cut(s) 44, 59, 134, 167, 263
BspCNI CTCAG 1 cut(s) 220
BspEI TCCGGA 1 cut(s) 491
BspFNI CGCG 2 cut(s) 218, 291
BspLI GGNNCC 5 cut(s) 44, 59, 396, 397, 475
BspPI GGATC 3 cut(s) 72, 88, 132
BspT107I GGYRCC 1 cut(s) 473
BspTI CTTAAG 1 cut(s) 332
BsrDI GCAATG 1 cut(s) 567
BsrFI RCCGGY 3 cut(s) 152, 163, 259
BsrI ACTGG 5 cut(s) 82, 257, 288, 609, 723
BssAI RCCGGY 3 cut(s) 152, 163, 259
BssECI CCNNGG 4 cut(s) 305, 306, 402, 429
BssMI GATC 4 cut(s) 77, 93, 124, 589
BssT1I CCWWGG 1 cut(s) 402
Bst2UI CCWGG 1 cut(s) 307
Bst4CI ACNGT 2 cut(s) 343, 416
BstAFI CTTAAG 1 cut(s) 332
BstC8I GCNNGC 4 cut(s) 261, 273, 289, 469
BstDEI CTNAG 1 cut(s) 207
BstDSI CCRYGG 1 cut(s) 429
BstF5I GGATG 2 cut(s) 439, 727
BstFNI CGCG 2 cut(s) 218, 291
BstHHI GCGC 1 cut(s) 220
BstKTI GATC 4 cut(s) 80, 96, 127, 592
BstMBI GATC 4 cut(s) 77, 93, 124, 589
BstMWI GCNNNNNNNGC 6 cut(s) 164, 284, 473, 482, 569, 581
BstNI CCWGG 1 cut(s) 307
BstNSI RCATGY 1 cut(s) 602
BstSCI CCNGG 3 cut(s) 44, 192, 305
BstSFI CTRYAG 1 cut(s) 612
BstSLI GKGCMC 2 cut(s) 46, 61
BstUI CGCG 2 cut(s) 218, 291
BstV1I GCAGC 1 cut(s) 559
BstX2I RGATCY 1 cut(s) 124
BstYI RGATCY 1 cut(s) 124
Bsu36I CCTNAGG 1 cut(s) 207
BsuRI GGCC 5 cut(s) 44, 59, 134, 167, 263
BtgI CCRYGG 1 cut(s) 429
BtsCI GGATG 2 cut(s) 439, 727
BtsI GCAGTG 2 cut(s) 444, 558
BtsIMutI CAGTG 3 cut(s) 339, 444, 558
Cac8I GCNNGC 4 cut(s) 261, 273, 289, 469
CfoI GCGC 1 cut(s) 220
Cfr10I RCCGGY 3 cut(s) 152, 163, 259
Cfr13I GGNCC 7 cut(s) 42, 43, 57, 58, 211, 395, 674
CseI GACGC 1 cut(s) 348
Csp6I GTAC 1 cut(s) 602
CviAII CATG 4 cut(s) 169, 265, 422, 599
CviQI GTAC 1 cut(s) 602
DdeI CTNAG 1 cut(s) 207
DpnI GATC 4 cut(s) 79, 95, 126, 591
DpnII GATC 4 cut(s) 77, 93, 124, 589
EaeI YGGCCR 3 cut(s) 132, 165, 261
EciI GGCGGA 3 cut(s) 58, 139, 235
Eco130I CCWWGG 1 cut(s) 402
Eco24I GRGCYC 2 cut(s) 46, 61
Eco47I GGWCC 3 cut(s) 211, 395, 674
Eco81I CCTNAGG 1 cut(s) 207
Eco88I CYCGRG 1 cut(s) 462
EcoO109I RGGNCCY 2 cut(s) 58, 395
EcoRII CCWGG 1 cut(s) 305
EcoT14I CCWWGG 1 cut(s) 402
EcoT22I ATGCAT 1 cut(s) 104
EcoT38I GRGCYC 2 cut(s) 46, 61
ErhI CCWWGG 1 cut(s) 402
FaeI CATG 4 cut(s) 172, 268, 425, 602
FaiI YATR 6 cut(s) 170, 266, 347, 423, 540, 600
FaqI GGGAC 1 cut(s) 408
FatI CATG 4 cut(s) 168, 264, 421, 598
FauI CCCGC 1 cut(s) 637
FbaI TGATCA 1 cut(s) 589
FblI GTMKAC 1 cut(s) 411
Fnu4HI GCNGC 3 cut(s) 89, 276, 573
FokI GGATG 2 cut(s) 446, 734
FriOI GRGCYC 2 cut(s) 46, 61
Fsp4HI GCNGC 3 cut(s) 89, 276, 573
GlaI GCGC 1 cut(s) 219
GluI GCNGC 3 cut(s) 89, 276, 573
HaeIII GGCC 5 cut(s) 44, 59, 134, 167, 263
HapII CCGG 7 cut(s) 46, 153, 164, 194, 260, 297, 492
HgaI GACGC 1 cut(s) 348
HhaI GCGC 1 cut(s) 220
Hin1II CATG 4 cut(s) 172, 268, 425, 602
Hin6I GCGC 1 cut(s) 218
HinP1I GCGC 1 cut(s) 218
HinfI GANTC 5 cut(s) 293, 425, 443, 691, 704
HpaII CCGG 7 cut(s) 46, 153, 164, 194, 260, 297, 492
HphI GGTGA 2 cut(s) 153, 745
Hpy166II GTNNAC 5 cut(s) 214, 412, 429, 502, 558
Hpy188I TCNGA 3 cut(s) 77, 129, 364
Hpy188III TCNNGA 3 cut(s) 314, 492, 765
Hpy8I GTNNAC 5 cut(s) 214, 412, 429, 502, 558
Hpy99I CGWCG 1 cut(s) 236
HpyAV CCTTC 1 cut(s) 719
HpyCH4III ACNGT 2 cut(s) 343, 416
HpyCH4IV ACGT 1 cut(s) 231
HpyCH4V TGCA 4 cut(s) 102, 158, 437, 485
HpyF10VI GCNNNNNNNGC 6 cut(s) 164, 284, 473, 482, 569, 581
HpyF3I CTNAG 1 cut(s) 207
HpySE526I ACGT 1 cut(s) 231
Hsp92II CATG 4 cut(s) 172, 268, 425, 602
HspAI GCGC 1 cut(s) 218
KflI GGGWCCC 1 cut(s) 395
Kpn2I TCCGGA 1 cut(s) 491
KroI GCCGGC 1 cut(s) 259
KroNI GCCGGC 1 cut(s) 261
Ksp22I TGATCA 1 cut(s) 589
Kzo9I GATC 4 cut(s) 77, 93, 124, 589
LmnI GCTCC 3 cut(s) 177, 328, 494
Lsp1109I GCAGC 1 cut(s) 559
LweI GCATC 3 cut(s) 111, 167, 424
MaeII ACGT 1 cut(s) 231
MaeIII GTNAC 1 cut(s) 508
MalI GATC 4 cut(s) 79, 95, 126, 591
MboI GATC 4 cut(s) 77, 93, 124, 589
MboII GAAGA 2 cut(s) 29, 197
MflI RGATCY 1 cut(s) 124
MhlI GDGCHC 3 cut(s) 46, 61, 538
MlsI TGGCCA 1 cut(s) 134
MluNI TGGCCA 1 cut(s) 134
MlyI GAGTC 2 cut(s) 302, 434
Mox20I TGGCCA 1 cut(s) 134
Mph1103I ATGCAT 1 cut(s) 104
MroI TCCGGA 1 cut(s) 491
MroNI GCCGGC 1 cut(s) 259
MscI TGGCCA 1 cut(s) 134
MseI TTAA 2 cut(s) 333, 747
Msp20I TGGCCA 1 cut(s) 134
MspA1I CMGCKG 3 cut(s) 91, 471, 617
MspCI CTTAAG 1 cut(s) 332
MspI CCGG 7 cut(s) 46, 153, 164, 194, 260, 297, 492
MspR9I CCNGG 3 cut(s) 46, 194, 307
MvaI CCWGG 1 cut(s) 307
MvnI CGCG 2 cut(s) 218, 291
MwoI GCNNNNNNNGC 6 cut(s) 164, 284, 473, 482, 569, 581
NaeI GCCGGC 1 cut(s) 261
NciI CCSGG 2 cut(s) 46, 194
NdeII GATC 4 cut(s) 77, 93, 124, 589
NgoMIV GCCGGC 1 cut(s) 259
NlaIII CATG 4 cut(s) 172, 268, 425, 602
NlaIV GGNNCC 5 cut(s) 44, 59, 396, 397, 475
NmuCI GTSAC 1 cut(s) 508
NsiI ATGCAT 1 cut(s) 104
NspI RCATGY 1 cut(s) 602
PaeR7I CTCGAG 1 cut(s) 462
PasI CCCWGGG 1 cut(s) 306
PciI ACATGT 1 cut(s) 598
PdiI GCCGGC 1 cut(s) 261
PfeI GAWTC 3 cut(s) 443, 691, 704
PflFI GACNNNGTC 1 cut(s) 506
PfoI TCCNGGA 1 cut(s) 192
PkrI GCNGC 3 cut(s) 90, 277, 574
PleI GAGTC 2 cut(s) 301, 433
PpsI GAGTC 2 cut(s) 301, 433
PpuMI RGGWCCY 1 cut(s) 395
PscI ACATGT 1 cut(s) 598
Psp5II RGGWCCY 1 cut(s) 395
Psp6I CCWGG 1 cut(s) 305
PspGI CCWGG 1 cut(s) 305
PspN4I GGNNCC 5 cut(s) 44, 59, 396, 397, 475
PspOMI GGGCCC 2 cut(s) 42, 57
PspPI GGNCC 7 cut(s) 42, 43, 57, 58, 211, 395, 674
PspPPI RGGWCCY 1 cut(s) 395
PspXI VCTCGAGB 1 cut(s) 462
PsuI RGATCY 1 cut(s) 124
PsyI GACNNNGTC 1 cut(s) 506
PvuII CAGCTG 1 cut(s) 617
RsaI GTAC 1 cut(s) 603
RsaNI GTAC 1 cut(s) 602
SaqAI TTAA 2 cut(s) 333, 747
SatI GCNGC 3 cut(s) 89, 276, 573
Sau3AI GATC 4 cut(s) 77, 93, 124, 589
Sau96I GGNCC 7 cut(s) 42, 43, 57, 58, 211, 395, 674
SchI GAGTC 2 cut(s) 302, 434
ScrFI CCNGG 3 cut(s) 46, 194, 307
SduI GDGCHC 3 cut(s) 46, 61, 538
SetI ASST 9 cut(s) 213, 234, 273, 333, 499, 613, 619, 686, 761
SfaNI GCATC 3 cut(s) 111, 167, 424
SfcI CTRYAG 1 cut(s) 612
Sfr274I CTCGAG 1 cut(s) 462
SgrAI CRCCGGYG 1 cut(s) 152
SinI GGWCC 3 cut(s) 211, 395, 674
SlaI CTCGAG 1 cut(s) 462
SmlI CTYRAG 3 cut(s) 332, 462, 685
SmoI CTYRAG 3 cut(s) 332, 462, 685
SsiI CCGC 7 cut(s) 69, 89, 150, 246, 276, 471, 644
StyD4I CCNGG 3 cut(s) 44, 192, 305
StyI CCWWGG 1 cut(s) 402
TaaI ACNGT 2 cut(s) 343, 416
TaiI ACGT 1 cut(s) 234
TaqI TCGA 3 cut(s) 183, 315, 463
TatI WGTACW 1 cut(s) 601
TauI GCSGC 2 cut(s) 91, 278
TfiI GAWTC 3 cut(s) 443, 691, 704
Tru1I TTAA 2 cut(s) 333, 747
Tru9I TTAA 2 cut(s) 333, 747
TscAI CASTG 3 cut(s) 346, 444, 565
TseFI GTSAC 1 cut(s) 508
TseI GCWGC 1 cut(s) 572
Tsp45I GTSAC 1 cut(s) 508
TspDTI ATGAA 2 cut(s) 281, 738
TspGWI ACGGA 2 cut(s) 446, 729
TspRI CASTG 3 cut(s) 346, 444, 565
Tth111I GACNNNGTC 1 cut(s) 506
Vha464I CTTAAG 1 cut(s) 332
VpaK11BI GGWCC 3 cut(s) 211, 395, 674
XceI RCATGY 1 cut(s) 602
XcmI CCANNNNNNNNNTGG 2 cut(s) 388, 436
XhoI CTCGAG 1 cut(s) 462
XmiI GTMKAC 1 cut(s) 411
Zsp2I ATGCAT 1 cut(s) 104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.