Rh4DG066000

Hydroxyethylthiazole

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0013360
Physical Location & Seq
Reverse (-)
9908749 .. 9910065
1317 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG066000.1

Sequence Viewer

Length: 843 bp
ATGGAAAGCAACAAGGAAGAACAAGCAGAGGCAACCCTCTGGGCCCGGAAGTCGTGGGCCCTACTCTCCGCCGTCCGATCCCAGTCGCCGCTGATCCAATGCATCACAAACTTCGTGTCAATGGATCTGATGGCCAACACTCTCTTATCCGCCGGTGCATCACCGGCCATGCTCCACGCCCTCGAAGAAATCCCGGACTTCACGCCTCAGGTCCACGCGCTCTGCCTCAACGTCGGCACACTCTCCGCCGACTGGTTTCCGGCCATGAAGCTCGCCGCCCAACTGGCCCGCAAGTCCGGTAAGCCCTGGGTTCTCGACCCTGTCGCCGCCGGCGCTTCCGGTTTCCGGTTGAAGGCTTGTTTGGAGCTTGTGGAGCTTAAGCCGACTGTTATACGAGGAAATGCTTCCGAGATTATCGCCCTCGCCAACGCTTCTGTGGGACCCACTAAGGGTGTAGACAGTTCCCATGAGTCCACGGATGCAGTGGAATCGGCAAAGTTATTGGCTCGAGCCAGCGGTGCCGTTGTTGCAGTTTCCGGAGCTGTGGACATCGTCACCAATGGGGAGAGAGTTGTTGGTGCTCATAATGGGGTTGCCATGATGACAAAGATTACGGCAACAGGGTGTTCTGTCACTGCTCTCATTGCTGCCTTTGTTGCTGTTGATCAAACACATGTACTGGAAGCTACAGCTGTGGCGTTATCCTTGTTTGGGATCGCGGGAGAGATGGGAATGGAAATGGCGAATGGTCCAGCAACCTTGCGGATTCACTTGATAGATTCACTCCACGGACTGGATGAAGCGTGTGTGCTTTCTCGTGTTAAAATCACCAGGTTGTCGTGA

Protein Analysis

280

Amino Acids

29.15

Weight (kDa)

5.8

Isoelectric Point (pI)

22.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HK PF02110 22 - 266 1e-71 Hydroxyethylthiazole kinase family
Phos_pyr_kin PF08543 91 - 230 3e-06 Phosphomethylpyrimidine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013360)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24030
fragaria_vesca FvH4_4g05510 FvH4_4g05510
malus_domestica MD13G1229700.v1.1
prunus_persica Prupe.1G056900_v2.0.a1
pyrus_communis pycom13g20240
rosa_chinensis RchiOBHm_Chr4g0396631
rosa_laevigata RLG00000009514
rosa_multiflora Rmu_sc0006586.1_g000020 Rmu_sc0010969.1_g000006
rosa_roxburghii Rroxscaffold_2G00122890 Rroxscaffold_5G00341480
rosa_rugosa Rorug03G0348300
rosa_samantha Rh4AG070800 Rh4BG068800 Rh4CG076600 Rh4DG066000
rosa_wichuraiana Rw4G005660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 518
AccB7I CCANNNNNTGG 1 cut(s) 793
AccI GTMKAC 1 cut(s) 456
AccII CGCG 2 cut(s) 218, 719
AccIII TCCGGA 1 cut(s) 536
AclWI GGATC 4 cut(s) 72, 88, 132, 722
AcoI YGGCCR 3 cut(s) 132, 165, 261
AfaI GTAC 1 cut(s) 678
AfiI CCNNNNNNNGG 6 cut(s) 252, 345, 352, 449, 711, 793
AflII CTTAAG 1 cut(s) 377
AflIII ACRYGT 1 cut(s) 673
AgsI TTSAA 1 cut(s) 352
AjnI CCWGG 2 cut(s) 305, 830
AjuI GAANNNNNNNTTGG 2 cut(s) 344, 376
AluBI AGCT 6 cut(s) 271, 367, 376, 542, 686, 692
AluI AGCT 6 cut(s) 271, 367, 376, 542, 686, 692
Alw21I GWGCWC 1 cut(s) 583
AlwI GGATC 4 cut(s) 72, 88, 132, 722
Ama87I CYCGRG 1 cut(s) 507
Aor13HI TCCGGA 1 cut(s) 536
AoxI GGCC 6 cut(s) 42, 57, 132, 165, 261, 285
ApaI GGGCCC 2 cut(s) 46, 61
ApeKI GCWGC 1 cut(s) 647
ArsI GACNNNNNNTTYG 2 cut(s) 101, 133
Asp700I GAANNNNTTC 1 cut(s) 403
AspLEI GCGC 2 cut(s) 220, 335
AspS9I GGNCC 8 cut(s) 42, 43, 57, 58, 211, 286, 440, 749
AsuC2I CCSGG 2 cut(s) 46, 194
AsuHPI GGTGA 3 cut(s) 153, 547, 820
AvaI CYCGRG 1 cut(s) 507
AvaII GGWCC 3 cut(s) 211, 440, 749
AxyI CCTNAGG 1 cut(s) 207
BaeGI GKGCMC 2 cut(s) 46, 61
BalI TGGCCA 1 cut(s) 134
BanI GGYRCC 1 cut(s) 518
BanII GRGCYC 2 cut(s) 46, 61
BauI CACGAG 1 cut(s) 816
Bbv12I GWGCWC 1 cut(s) 583
BbvI GCAGC 1 cut(s) 634
BccI CCATC 2 cut(s) 124, 721
BceAI ACGGC 3 cut(s) 56, 506, 630
BcgI CGANNNNNNTGC 2 cut(s) 471, 505
BciT130I CCWGG 2 cut(s) 307, 832
BclI TGATCA 1 cut(s) 664
BcnI CCSGG 2 cut(s) 46, 194
BfmI CTRYAG 1 cut(s) 687
BfoI RGCGCY 1 cut(s) 336
BfrI CTTAAG 1 cut(s) 377
BglI GCCNNNNNGGC 1 cut(s) 284
BisI GCNGC 4 cut(s) 89, 276, 327, 648
BlsI GCNGC 4 cut(s) 90, 277, 328, 649
Bme1390I CCNGG 4 cut(s) 46, 194, 307, 832
Bme18I GGWCC 3 cut(s) 211, 440, 749
BmeT110I CYCGRG 1 cut(s) 507
BmgT120I GGNCC 8 cut(s) 42, 43, 57, 58, 211, 286, 440, 749
BmiI GGNNCC 5 cut(s) 44, 59, 441, 442, 520
BmrFI CCNGG 4 cut(s) 46, 194, 307, 832
BmrI ACTGGG 1 cut(s) 76
BmsI GCATC 3 cut(s) 111, 167, 469
BmuI ACTGGG 1 cut(s) 76
BpuMI CCSGG 2 cut(s) 46, 194
BsaJI CCNNGG 4 cut(s) 305, 306, 474, 787
BsaWI WCCGGW 4 cut(s) 296, 338, 345, 536
Bsc4I CCNNNNNNNGG 6 cut(s) 252, 345, 352, 449, 711, 793
Bse118I RCCGGY 3 cut(s) 152, 163, 329
Bse1I ACTGG 5 cut(s) 82, 257, 288, 684, 798
Bse21I CCTNAGG 1 cut(s) 207
Bse3DI GCAATG 1 cut(s) 642
BseAI TCCGGA 1 cut(s) 536
BseBI CCWGG 2 cut(s) 307, 832
BseDI CCNNGG 4 cut(s) 305, 306, 474, 787
BseGI GGATG 2 cut(s) 484, 802
BseLI CCNNNNNNNGG 6 cut(s) 252, 345, 352, 449, 711, 793
BseMI GCAATG 1 cut(s) 642
BseMII CTCAG 1 cut(s) 221
BseNI ACTGG 5 cut(s) 82, 257, 288, 684, 798
BseSI GKGCMC 2 cut(s) 46, 61
BseXI GCAGC 1 cut(s) 634
Bsh1236I CGCG 2 cut(s) 218, 719
BshFI GGCC 6 cut(s) 44, 59, 134, 167, 263, 287
BshNI GGYRCC 1 cut(s) 518
BsiHKAI GWGCWC 1 cut(s) 583
BsiHKCI CYCGRG 1 cut(s) 507
BslFI GGGAC 1 cut(s) 453
BslI CCNNNNNNNGG 6 cut(s) 252, 345, 352, 449, 711, 793
BsmFI GGGAC 1 cut(s) 453
BsnI GGCC 6 cut(s) 44, 59, 134, 167, 263, 287
BsoBI CYCGRG 1 cut(s) 507
Bsp120I GGGCCC 2 cut(s) 42, 57
Bsp1286I GDGCHC 3 cut(s) 46, 61, 583
Bsp13I TCCGGA 1 cut(s) 536
Bsp143I GATC 5 cut(s) 77, 93, 124, 664, 714
BspANI GGCC 6 cut(s) 44, 59, 134, 167, 263, 287
BspCNI CTCAG 1 cut(s) 220
BspEI TCCGGA 1 cut(s) 536
BspFNI CGCG 2 cut(s) 218, 719
BspLI GGNNCC 5 cut(s) 44, 59, 441, 442, 520
BspPI GGATC 4 cut(s) 72, 88, 132, 722
BspT107I GGYRCC 1 cut(s) 518
BspTI CTTAAG 1 cut(s) 377
BsrDI GCAATG 1 cut(s) 642
BsrFI RCCGGY 3 cut(s) 152, 163, 329
BsrI ACTGG 5 cut(s) 82, 257, 288, 684, 798
BssAI RCCGGY 3 cut(s) 152, 163, 329
BssECI CCNNGG 4 cut(s) 305, 306, 474, 787
BssMI GATC 5 cut(s) 77, 93, 124, 664, 714
BssSI CACGAG 1 cut(s) 816
Bst2BI CACGAG 1 cut(s) 816
Bst2UI CCWGG 2 cut(s) 307, 832
Bst4CI ACNGT 2 cut(s) 388, 461
BstAFI CTTAAG 1 cut(s) 377
BstC8I GCNNGC 4 cut(s) 273, 289, 331, 514
BstDEI CTNAG 2 cut(s) 207, 447
BstDSI CCRYGG 2 cut(s) 474, 787
BstF5I GGATG 2 cut(s) 484, 802
BstFNI CGCG 2 cut(s) 218, 719
BstH2I RGCGCY 1 cut(s) 336
BstHHI GCGC 2 cut(s) 220, 335
BstKTI GATC 5 cut(s) 80, 96, 127, 667, 717
BstMBI GATC 5 cut(s) 77, 93, 124, 664, 714
BstMWI GCNNNNNNNGC 8 cut(s) 164, 284, 332, 373, 518, 527, 644, 656
BstNI CCWGG 2 cut(s) 307, 832
BstNSI RCATGY 1 cut(s) 677
BstSCI CCNGG 4 cut(s) 44, 192, 305, 830
BstSFI CTRYAG 1 cut(s) 687
BstSLI GKGCMC 2 cut(s) 46, 61
BstUI CGCG 2 cut(s) 218, 719
BstV1I GCAGC 1 cut(s) 634
BstX2I RGATCY 1 cut(s) 124
BstYI RGATCY 1 cut(s) 124
Bsu36I CCTNAGG 1 cut(s) 207
BsuRI GGCC 6 cut(s) 44, 59, 134, 167, 263, 287
BtgI CCRYGG 2 cut(s) 474, 787
BtsCI GGATG 2 cut(s) 484, 802
BtsI GCAGTG 2 cut(s) 489, 633
BtsIMutI CAGTG 2 cut(s) 489, 633
Cac8I GCNNGC 4 cut(s) 273, 289, 331, 514
CfoI GCGC 2 cut(s) 220, 335
Cfr10I RCCGGY 3 cut(s) 152, 163, 329
Cfr13I GGNCC 8 cut(s) 42, 43, 57, 58, 211, 286, 440, 749
CsiI ACCWGGT 1 cut(s) 830
Csp6I GTAC 1 cut(s) 677
CviAII CATG 5 cut(s) 169, 265, 467, 598, 674
CviQI GTAC 1 cut(s) 677
DdeI CTNAG 2 cut(s) 207, 447
DpnI GATC 5 cut(s) 79, 95, 126, 666, 716
DpnII GATC 5 cut(s) 77, 93, 124, 664, 714
EaeI YGGCCR 3 cut(s) 132, 165, 261
EciI GGCGGA 3 cut(s) 58, 139, 235
Eco24I GRGCYC 2 cut(s) 46, 61
Eco47I GGWCC 3 cut(s) 211, 440, 749
Eco81I CCTNAGG 1 cut(s) 207
Eco88I CYCGRG 1 cut(s) 507
EcoO109I RGGNCCY 2 cut(s) 58, 440
EcoRII CCWGG 2 cut(s) 305, 830
EcoT22I ATGCAT 1 cut(s) 104
EcoT38I GRGCYC 2 cut(s) 46, 61
FaeI CATG 5 cut(s) 172, 268, 470, 601, 677
FaiI YATR 7 cut(s) 170, 266, 392, 468, 585, 599, 675
FaqI GGGAC 1 cut(s) 453
FatI CATG 5 cut(s) 168, 264, 466, 597, 673
FauI CCCGC 2 cut(s) 296, 712
FbaI TGATCA 1 cut(s) 664
FblI GTMKAC 1 cut(s) 456
Fnu4HI GCNGC 4 cut(s) 89, 276, 327, 648
FokI GGATG 2 cut(s) 491, 809
FriOI GRGCYC 2 cut(s) 46, 61
Fsp4HI GCNGC 4 cut(s) 89, 276, 327, 648
GlaI GCGC 2 cut(s) 219, 334
GluI GCNGC 4 cut(s) 89, 276, 327, 648
HaeII RGCGCY 1 cut(s) 336
HaeIII GGCC 6 cut(s) 44, 59, 134, 167, 263, 287
HhaI GCGC 2 cut(s) 220, 335
Hin1II CATG 5 cut(s) 172, 268, 470, 601, 677
Hin6I GCGC 2 cut(s) 218, 333
HinP1I GCGC 2 cut(s) 218, 333
HinfI GANTC 4 cut(s) 470, 488, 766, 779
HphI GGTGA 3 cut(s) 153, 547, 820
Hpy166II GTNNAC 4 cut(s) 214, 457, 474, 547
Hpy188I TCNGA 3 cut(s) 77, 129, 409
Hpy188III TCNNGA 3 cut(s) 314, 537, 840
Hpy8I GTNNAC 4 cut(s) 214, 457, 474, 547
Hpy99I CGWCG 1 cut(s) 236
HpyAV CCTTC 1 cut(s) 346
HpyCH4III ACNGT 2 cut(s) 388, 461
HpyCH4IV ACGT 1 cut(s) 231
HpyCH4V TGCA 4 cut(s) 102, 158, 482, 530
HpyF10VI GCNNNNNNNGC 8 cut(s) 164, 284, 332, 373, 518, 527, 644, 656
HpyF3I CTNAG 2 cut(s) 207, 447
HpySE526I ACGT 1 cut(s) 231
Hsp92II CATG 5 cut(s) 172, 268, 470, 601, 677
HspAI GCGC 2 cut(s) 218, 333
KflI GGGWCCC 1 cut(s) 440
Kpn2I TCCGGA 1 cut(s) 536
KroI GCCGGC 1 cut(s) 329
KroNI GCCGGC 1 cut(s) 331
Ksp22I TGATCA 1 cut(s) 664
Kzo9I GATC 5 cut(s) 77, 93, 124, 664, 714
LmnI GCTCC 4 cut(s) 177, 364, 373, 539
Lsp1109I GCAGC 1 cut(s) 634
LweI GCATC 3 cut(s) 111, 167, 469
MabI ACCWGGT 1 cut(s) 830
MaeII ACGT 1 cut(s) 231
MaeIII GTNAC 2 cut(s) 553, 631
MalI GATC 5 cut(s) 79, 95, 126, 666, 716
MboI GATC 5 cut(s) 77, 93, 124, 664, 714
MboII GAAGA 2 cut(s) 29, 197
MflI RGATCY 1 cut(s) 124
MhlI GDGCHC 3 cut(s) 46, 61, 583
MlsI TGGCCA 1 cut(s) 134
MluNI TGGCCA 1 cut(s) 134
MlyI GAGTC 1 cut(s) 479
MnlI CCTC 7 cut(s) 22, 47, 191, 216, 236, 389, 431
Mox20I TGGCCA 1 cut(s) 134
Mph1103I ATGCAT 1 cut(s) 104
MreI CGCCGGCG 1 cut(s) 329
MroI TCCGGA 1 cut(s) 536
MroNI GCCGGC 1 cut(s) 329
MroXI GAANNNNTTC 1 cut(s) 403
MscI TGGCCA 1 cut(s) 134
MseI TTAA 2 cut(s) 378, 822
Msp20I TGGCCA 1 cut(s) 134
MspA1I CMGCKG 3 cut(s) 91, 516, 692
MspCI CTTAAG 1 cut(s) 377
MspR9I CCNGG 4 cut(s) 46, 194, 307, 832
MvaI CCWGG 2 cut(s) 307, 832
MvnI CGCG 2 cut(s) 218, 719
MwoI GCNNNNNNNGC 8 cut(s) 164, 284, 332, 373, 518, 527, 644, 656
NaeI GCCGGC 1 cut(s) 331
NciI CCSGG 2 cut(s) 46, 194
NdeII GATC 5 cut(s) 77, 93, 124, 664, 714
NgoMIV GCCGGC 1 cut(s) 329
NlaIII CATG 5 cut(s) 172, 268, 470, 601, 677
NlaIV GGNNCC 5 cut(s) 44, 59, 441, 442, 520
NmuCI GTSAC 2 cut(s) 553, 631
NsiI ATGCAT 1 cut(s) 104
NspI RCATGY 1 cut(s) 677
PaeR7I CTCGAG 1 cut(s) 507
PasI CCCWGGG 1 cut(s) 306
PciI ACATGT 1 cut(s) 673
PdiI GCCGGC 1 cut(s) 331
PdmI GAANNNNTTC 1 cut(s) 403
PfeI GAWTC 3 cut(s) 488, 766, 779
PflFI GACNNNGTC 2 cut(s) 320, 551
PflMI CCANNNNNTGG 1 cut(s) 793
PfoI TCCNGGA 1 cut(s) 192
PkrI GCNGC 4 cut(s) 90, 277, 328, 649
PleI GAGTC 1 cut(s) 478
PpsI GAGTC 1 cut(s) 478
PpuMI RGGWCCY 1 cut(s) 440
PscI ACATGT 1 cut(s) 673
Psp5II RGGWCCY 1 cut(s) 440
Psp6I CCWGG 2 cut(s) 305, 830
PspGI CCWGG 2 cut(s) 305, 830
PspN4I GGNNCC 5 cut(s) 44, 59, 441, 442, 520
PspOMI GGGCCC 2 cut(s) 42, 57
PspPI GGNCC 8 cut(s) 42, 43, 57, 58, 211, 286, 440, 749
PspPPI RGGWCCY 1 cut(s) 440
PspXI VCTCGAGB 1 cut(s) 507
PsuI RGATCY 1 cut(s) 124
PsyI GACNNNGTC 2 cut(s) 320, 551
PvuII CAGCTG 1 cut(s) 692
RsaI GTAC 1 cut(s) 678
RsaNI GTAC 1 cut(s) 677
SaqAI TTAA 2 cut(s) 378, 822
SatI GCNGC 4 cut(s) 89, 276, 327, 648
Sau3AI GATC 5 cut(s) 77, 93, 124, 664, 714
Sau96I GGNCC 8 cut(s) 42, 43, 57, 58, 211, 286, 440, 749
SchI GAGTC 1 cut(s) 479
ScrFI CCNGG 4 cut(s) 46, 194, 307, 832
SduI GDGCHC 3 cut(s) 46, 61, 583
SexAI ACCWGGT 1 cut(s) 830
SfaNI GCATC 3 cut(s) 111, 167, 469
SfcI CTRYAG 1 cut(s) 687
Sfr274I CTCGAG 1 cut(s) 507
SgrAI CRCCGGYG 2 cut(s) 152, 329
SinI GGWCC 3 cut(s) 211, 440, 749
SlaI CTCGAG 1 cut(s) 507
SmlI CTYRAG 2 cut(s) 377, 507
SmoI CTYRAG 2 cut(s) 377, 507
StyD4I CCNGG 4 cut(s) 44, 192, 305, 830
TaaI ACNGT 2 cut(s) 388, 461
TaiI ACGT 1 cut(s) 234
TaqI TCGA 3 cut(s) 183, 315, 508
TatI WGTACW 1 cut(s) 676
TauI GCSGC 3 cut(s) 91, 278, 329
TfiI GAWTC 3 cut(s) 488, 766, 779
Tru1I TTAA 2 cut(s) 378, 822
Tru9I TTAA 2 cut(s) 378, 822
TscAI CASTG 2 cut(s) 489, 640
TseFI GTSAC 2 cut(s) 553, 631
TseI GCWGC 1 cut(s) 647
Tsp45I GTSAC 2 cut(s) 553, 631
TspDTI ATGAA 2 cut(s) 281, 813
TspGWI ACGGA 2 cut(s) 491, 804
TspRI CASTG 2 cut(s) 489, 640
Tth111I GACNNNGTC 2 cut(s) 320, 551
Van91I CCANNNNNTGG 1 cut(s) 793
Vha464I CTTAAG 1 cut(s) 377
VpaK11BI GGWCC 3 cut(s) 211, 440, 749
XceI RCATGY 1 cut(s) 677
XcmI CCANNNNNNNNNTGG 2 cut(s) 433, 481
XhoI CTCGAG 1 cut(s) 507
XmiI GTMKAC 1 cut(s) 456
XmnI GAANNNNTTC 1 cut(s) 403
Zsp2I ATGCAT 1 cut(s) 104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.