Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0013360

Physical Location & Seq

Genomic Coordinates Forward (+)

22542809 .. 22544555

Gene Structure 1747 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1229700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 858 bp

ATGGAGAGCAAGTCCAACTTCAAGGCAGAAGAAGAAGATCCAGAGGCAATCGTGTGGGCCCAGAAAGCATGGGCCCTACTATCTTCAGTTCGAGCCAAATCACCGCTCATCCAGTGCATCACCAACTTCGTGTCAATGGATCTGATGGCCAACACTCTCTTGTCCGCGGGTGCATCACCCGCCATGCTCCACTCCATAGAAGAACTCCCCGACTTCACGCCTCAGGTCCACGCGCTCTGCGTCAACGTGGGCACGCTCTCCGCCGACTGGTTACCGGCCATGAAGGTCGCCGCTGAACTGGCGCACAACTCCGGCAAGCCTTGGGTTCTCGATCCAGTTGCCGCCGGCGCCTCAGGTTTCCGGTTGAAGGCTTGTTTGGAGCTTGTGGAGCTTAAGCCTACTGTTATTAGGGGAAATGCGTCCGAGATTATCGCCCTCGCCAATGCTTCTGTGGGACCTACCAAGGGGGCAGACAGTTCCCATGAATCAACAGATGCAGTTGAATCAGCAAAATCACTGGCTCAATCCAGTGGTGCCTTAGTTGCGGTTTCTGGAGCTGTCGACATTGTTACAGATGGGAAGAGAGTTGTTGGTGCTCGTAATGGGGTTGCCATGTTGACAAAGATCACGGCAACCGGATGTTCAGTCACTGCCCTGATTGCTGCCTTTGTTGCTGTTGATCAATCTCATGTTTTGGAAGCTACAGCTTCTGCATTATCGATATTTGGGATTGCGAGCGAGGTGGGAATGAAACTGGCAAAGGGTCCAGCTTCATTGCGGATGCACTTGATAGATTCACTCTACAGGCTCGATGAAGTATGTGTGCTTTCTCATGTTAAAATCGGCAGCTTGTCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004417 GO:0006139 GO:0006725 GO:0006766 GO:0006767 GO:0006772 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008655 GO:0009058 GO:0009110 GO:0009228 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019438 GO:0034641 GO:0034654 GO:0036172 GO:0042364 GO:0042723 GO:0042724 GO:0043094 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044283 GO:0046483 GO:0071704 GO:0072527 GO:0072528 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00730 ko01100 map00730 map01100

Pfam Domains

Protein Families

Protein Analysis

286

Amino Acids

29.54

Weight (kDa)

5.65

Isoelectric Point (pI)

24.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HK	PF02110	27 - 271	2.8e-70	Hydroxyethylthiazole kinase family
Phos_pyr_kin	PF08543	95 - 240	2.9e-06	Phosphomethylpyrimidine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013360)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G24030
fragaria_vesca	FvH4_4g05510 FvH4_4g05510
malus_domestica	MD13G1229700.v1.1
prunus_persica	Prupe.1G056900_v2.0.a1
pyrus_communis	pycom13g20240
rosa_chinensis	RchiOBHm_Chr4g0396631
rosa_laevigata	RLG00000009514
rosa_multiflora	Rmu_sc0006586.1_g000020 Rmu_sc0010969.1_g000006
rosa_roxburghii	Rroxscaffold_2G00122890 Rroxscaffold_5G00341480
rosa_rugosa	Rorug03G0348300
rosa_samantha	Rh4AG070800 Rh4BG068800 Rh4CG076600 Rh4DG066000
rosa_wichuraiana	Rw4G005660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	347, 533
AccBSI	CCGCTC	1 cut(s)	106
AccI	GTMKAC	1 cut(s)	561
AccII	CGCG	2 cut(s)	167, 233
AciI	CCGC	9 cut(s)	104, 165, 167, 180, 261, 291, 342, 545, 778
AclWI	GGATC	3 cut(s)	32, 147, 326
AcoI	YGGCCR	2 cut(s)	147, 276
AcuI	CTGAAG	1 cut(s)	69
AcyI	GRCGYC	1 cut(s)	348
AfiI	CCNNNNNNNGG	3 cut(s)	267, 367, 464
AflII	CTTAAG	1 cut(s)	392
AgsI	TTSAA	3 cut(s)	22, 367, 503
AjuI	GAANNNNNNNTTGG	2 cut(s)	359, 391
AluBI	AGCT	7 cut(s)	382, 391, 557, 701, 707, 770, 849
AluI	AGCT	7 cut(s)	382, 391, 557, 701, 707, 770, 849
Alw21I	GWGCWC	1 cut(s)	598
AlwI	GGATC	3 cut(s)	32, 147, 326
AlwNI	CAGNNNCTG	2 cut(s)	650, 710
AoxI	GGCC	4 cut(s)	57, 72, 147, 276
ApaI	GGGCCC	2 cut(s)	61, 76
ApeKI	GCWGC	2 cut(s)	662, 846
AspLEI	GCGC	3 cut(s)	235, 304, 350
AspS9I	GGNCC	7 cut(s)	57, 58, 72, 73, 226, 455, 764
AsuHPI	GGTGA	3 cut(s)	93, 112, 168
AvaII	GGWCC	3 cut(s)	226, 455, 764
AxyI	CCTNAGG	2 cut(s)	222, 352
BaeGI	GKGCMC	3 cut(s)	61, 76, 254
BalI	TGGCCA	1 cut(s)	149
BanI	GGYRCC	2 cut(s)	347, 533
BanII	GRGCYC	2 cut(s)	61, 76
Bbv12I	GWGCWC	1 cut(s)	598
BbvI	GCAGC	1 cut(s)	649
BccI	CCATC	2 cut(s)	139, 569
BceAI	ACGGC	1 cut(s)	645
BcgI	CGANNNNNNTGC	2 cut(s)	320, 354
BclI	TGATCA	1 cut(s)	679
BfmI	CTRYAG	2 cut(s)	702, 802
BfoI	RGCGCY	1 cut(s)	351
BfrI	CTTAAG	1 cut(s)	392
BisI	GCNGC	4 cut(s)	291, 342, 663, 847
BlsI	GCNGC	4 cut(s)	292, 343, 664, 848
Bme18I	GGWCC	3 cut(s)	226, 455, 764
BmgT120I	GGNCC	7 cut(s)	57, 58, 72, 73, 226, 455, 764
BmiI	GGNNCC	6 cut(s)	59, 74, 349, 456, 535, 765
BmsI	GCATC	4 cut(s)	126, 182, 484, 771
BpmI	CTGGAG	1 cut(s)	573
Bsa29I	ATCGAT	1 cut(s)	719
BsaHI	GRCGYC	1 cut(s)	348
BsaJI	CCNNGG	3 cut(s)	165, 320, 462
BsaWI	WCCGGW	2 cut(s)	360, 635
BsaXI	ACNNNNNCTCC	1 cut(s)	26
Bsc4I	CCNNNNNNNGG	3 cut(s)	267, 367, 464
Bse118I	RCCGGY	2 cut(s)	274, 344
Bse1I	ACTGG	7 cut(s)	112, 272, 303, 335, 522, 528, 759
Bse21I	CCTNAGG	2 cut(s)	222, 352
Bse3DI	GCAATG	1 cut(s)	773
BseCI	ATCGAT	1 cut(s)	719
BseDI	CCNNGG	3 cut(s)	165, 320, 462
BseGI	GGATG	3 cut(s)	108, 644, 786
BseLI	CCNNNNNNNGG	3 cut(s)	267, 367, 464
BseMI	GCAATG	1 cut(s)	773
BseMII	CTCAG	2 cut(s)	236, 366
BseNI	ACTGG	7 cut(s)	112, 272, 303, 335, 522, 528, 759
BseSI	GKGCMC	3 cut(s)	61, 76, 254
BseXI	GCAGC	1 cut(s)	649
Bsh1236I	CGCG	2 cut(s)	167, 233
BshFI	GGCC	4 cut(s)	59, 74, 149, 278
BshNI	GGYRCC	2 cut(s)	347, 533
BshVI	ATCGAT	1 cut(s)	719
BsiHKAI	GWGCWC	1 cut(s)	598
BsiSI	CCGG	5 cut(s)	275, 312, 345, 361, 636
BslFI	GGGAC	1 cut(s)	468
BslI	CCNNNNNNNGG	3 cut(s)	267, 367, 464
BsmFI	GGGAC	1 cut(s)	468
BsnI	GGCC	4 cut(s)	59, 74, 149, 278
Bsp120I	GGGCCC	2 cut(s)	57, 72
Bsp1286I	GDGCHC	4 cut(s)	61, 76, 254, 598
Bsp143I	GATC	5 cut(s)	37, 139, 331, 624, 679
BspACI	CCGC	9 cut(s)	104, 165, 167, 180, 261, 291, 342, 545, 778
BspANI	GGCC	4 cut(s)	59, 74, 149, 278
BspCNI	CTCAG	2 cut(s)	235, 365
BspDI	ATCGAT	1 cut(s)	719
BspFNI	CGCG	2 cut(s)	167, 233
BspHI	TCATGA	1 cut(s)	854
BspLI	GGNNCC	6 cut(s)	59, 74, 349, 456, 535, 765
BspPI	GGATC	3 cut(s)	32, 147, 326
BspT107I	GGYRCC	2 cut(s)	347, 533
BspTI	CTTAAG	1 cut(s)	392
BsrBI	CCGCTC	1 cut(s)	106
BsrDI	GCAATG	1 cut(s)	773
BsrFI	RCCGGY	2 cut(s)	274, 344
BsrI	ACTGG	7 cut(s)	112, 272, 303, 335, 522, 528, 759
BssAI	RCCGGY	2 cut(s)	274, 344
BssECI	CCNNGG	3 cut(s)	165, 320, 462
BssMI	GATC	5 cut(s)	37, 139, 331, 624, 679
BssNI	GRCGYC	1 cut(s)	348
BssT1I	CCWWGG	2 cut(s)	320, 462
Bst4CI	ACNGT	2 cut(s)	403, 476
Bst6I	CTCTTC	1 cut(s)	575
BstACI	GRCGYC	1 cut(s)	348
BstAFI	CTTAAG	1 cut(s)	392
BstC8I	GCNNGC	4 cut(s)	254, 317, 346, 736
BstDEI	CTNAG	3 cut(s)	222, 352, 538
BstDSI	CCRYGG	1 cut(s)	165
BstEII	GGTNACC	1 cut(s)	270
BstF5I	GGATG	3 cut(s)	108, 644, 786
BstFNI	CGCG	2 cut(s)	167, 233
BstH2I	RGCGCY	1 cut(s)	351
BstHHI	GCGC	3 cut(s)	235, 304, 350
BstKTI	GATC	5 cut(s)	40, 142, 334, 627, 682
BstMBI	GATC	5 cut(s)	37, 139, 331, 624, 679
BstMWI	GCNNNNNNNGC	8 cut(s)	65, 179, 299, 347, 388, 542, 659, 671
BstPI	GGTNACC	1 cut(s)	270
BstSFI	CTRYAG	2 cut(s)	702, 802
BstSLI	GKGCMC	3 cut(s)	61, 76, 254
BstUI	CGCG	2 cut(s)	167, 233
BstV1I	GCAGC	1 cut(s)	649
BstX2I	RGATCY	2 cut(s)	37, 139
BstYI	RGATCY	2 cut(s)	37, 139
Bsu15I	ATCGAT	1 cut(s)	719
Bsu36I	CCTNAGG	2 cut(s)	222, 352
BsuRI	GGCC	4 cut(s)	59, 74, 149, 278
BsuTUI	ATCGAT	1 cut(s)	719
BtgI	CCRYGG	1 cut(s)	165
BtsCI	GGATG	3 cut(s)	108, 644, 786
BtsI	GCAGTG	1 cut(s)	648
BtsIMutI	CAGTG	4 cut(s)	119, 515, 535, 648
Cac8I	GCNNGC	4 cut(s)	254, 317, 346, 736
CaiI	CAGNNNCTG	2 cut(s)	650, 710
CciI	TCATGA	1 cut(s)	854
CfoI	GCGC	3 cut(s)	235, 304, 350
Cfr10I	RCCGGY	2 cut(s)	274, 344
Cfr13I	GGNCC	7 cut(s)	57, 58, 72, 73, 226, 455, 764
Cfr42I	CCGCGG	1 cut(s)	168
ClaI	ATCGAT	1 cut(s)	719
CseI	GACGC	2 cut(s)	229, 408
CviAII	CATG	8 cut(s)	69, 184, 280, 482, 613, 689, 833, 855
DdeI	CTNAG	3 cut(s)	222, 352, 538
DinI	GGCGCC	1 cut(s)	349
DpnI	GATC	5 cut(s)	39, 141, 333, 626, 681
DpnII	GATC	5 cut(s)	37, 139, 331, 624, 679
EaeI	YGGCCR	2 cut(s)	147, 276
Eam1104I	CTCTTC	1 cut(s)	575
EarI	CTCTTC	1 cut(s)	575
EciI	GGCGGA	1 cut(s)	250
Eco130I	CCWWGG	2 cut(s)	320, 462
Eco24I	GRGCYC	2 cut(s)	61, 76
Eco47I	GGWCC	3 cut(s)	226, 455, 764
Eco57I	CTGAAG	1 cut(s)	69
Eco81I	CCTNAGG	2 cut(s)	222, 352
Eco91I	GGTNACC	1 cut(s)	270
EcoO109I	RGGNCCY	2 cut(s)	73, 455
EcoO65I	GGTNACC	1 cut(s)	270
EcoT14I	CCWWGG	2 cut(s)	320, 462
EcoT38I	GRGCYC	2 cut(s)	61, 76
EgeI	GGCGCC	1 cut(s)	349
EheI	GGCGCC	1 cut(s)	349
ErhI	CCWWGG	2 cut(s)	320, 462
FaeI	CATG	8 cut(s)	72, 187, 283, 485, 616, 692, 836, 858
FalI	AAGNNNNNCTT	1 cut(s)	34
FaqI	GGGAC	1 cut(s)	468
FatI	CATG	8 cut(s)	68, 183, 279, 481, 612, 688, 832, 854
FauI	CCCGC	2 cut(s)	160, 187
FbaI	TGATCA	1 cut(s)	679
FblI	GTMKAC	1 cut(s)	561
Fnu4HI	GCNGC	4 cut(s)	291, 342, 663, 847
FokI	GGATG	3 cut(s)	95, 651, 793
FriOI	GRGCYC	2 cut(s)	61, 76
Fsp4HI	GCNGC	4 cut(s)	291, 342, 663, 847
GlaI	GCGC	3 cut(s)	234, 303, 349
GluI	GCNGC	4 cut(s)	291, 342, 663, 847
GsuI	CTGGAG	1 cut(s)	573
HaeII	RGCGCY	1 cut(s)	351
HaeIII	GGCC	4 cut(s)	59, 74, 149, 278
HapII	CCGG	5 cut(s)	275, 312, 345, 361, 636
HgaI	GACGC	2 cut(s)	229, 408
HhaI	GCGC	3 cut(s)	235, 304, 350
Hin1I	GRCGYC	1 cut(s)	348
Hin1II	CATG	8 cut(s)	72, 187, 283, 485, 616, 692, 836, 858
Hin6I	GCGC	3 cut(s)	233, 302, 348
HinP1I	GCGC	3 cut(s)	233, 302, 348
HincII	GTYRAC	3 cut(s)	244, 562, 618
HindII	GTYRAC	3 cut(s)	244, 562, 618
HinfI	GANTC	3 cut(s)	485, 503, 794
HpaII	CCGG	5 cut(s)	275, 312, 345, 361, 636
HphI	GGTGA	3 cut(s)	93, 112, 168
Hpy166II	GTNNAC	4 cut(s)	229, 244, 562, 618
Hpy188I	TCNGA	2 cut(s)	144, 424
Hpy188III	TCNNGA	4 cut(s)	41, 329, 552, 855
Hpy8I	GTNNAC	4 cut(s)	229, 244, 562, 618
HpyAV	CCTTC	2 cut(s)	277, 361
HpyCH4III	ACNGT	2 cut(s)	403, 476
HpyCH4IV	ACGT	1 cut(s)	246
HpyCH4V	TGCA	5 cut(s)	117, 173, 497, 713, 784
HpyF10VI	GCNNNNNNNGC	8 cut(s)	65, 179, 299, 347, 388, 542, 659, 671
HpyF3I	CTNAG	3 cut(s)	222, 352, 538
HpySE526I	ACGT	1 cut(s)	246
Hsp92I	GRCGYC	1 cut(s)	348
Hsp92II	CATG	8 cut(s)	72, 187, 283, 485, 616, 692, 836, 858
HspAI	GCGC	3 cut(s)	233, 302, 348
KasI	GGCGCC	1 cut(s)	347
KroI	GCCGGC	1 cut(s)	344
KroNI	GCCGGC	1 cut(s)	346
Ksp22I	TGATCA	1 cut(s)	679
KspI	CCGCGG	1 cut(s)	168
Kzo9I	GATC	5 cut(s)	37, 139, 331, 624, 679
LmnI	GCTCC	4 cut(s)	192, 379, 388, 554
Lsp1109I	GCAGC	1 cut(s)	649
LweI	GCATC	4 cut(s)	126, 182, 484, 771
MaeII	ACGT	1 cut(s)	246
MaeIII	GTNAC	3 cut(s)	270, 568, 646
MalI	GATC	5 cut(s)	39, 141, 333, 626, 681
MbiI	CCGCTC	1 cut(s)	106
MboI	GATC	5 cut(s)	37, 139, 331, 624, 679
MboII	GAAGA	6 cut(s)	41, 44, 47, 75, 212, 592
MflI	RGATCY	2 cut(s)	37, 139
MhlI	GDGCHC	4 cut(s)	61, 76, 254, 598
MlsI	TGGCCA	1 cut(s)	149
MluNI	TGGCCA	1 cut(s)	149
Mly113I	GGCGCC	1 cut(s)	348
MmeI	TCCRAC	1 cut(s)	39
MnlI	CCTC	5 cut(s)	37, 231, 361, 446, 733
Mox20I	TGGCCA	1 cut(s)	149
MreI	CGCCGGCG	1 cut(s)	344
MroNI	GCCGGC	1 cut(s)	344
MscI	TGGCCA	1 cut(s)	149
MseI	TTAA	2 cut(s)	393, 837
Msp20I	TGGCCA	1 cut(s)	149
MspA1I	CMGCKG	2 cut(s)	167, 293
MspCI	CTTAAG	1 cut(s)	392
MspI	CCGG	5 cut(s)	275, 312, 345, 361, 636
MvnI	CGCG	2 cut(s)	167, 233
MwoI	GCNNNNNNNGC	8 cut(s)	65, 179, 299, 347, 388, 542, 659, 671
NaeI	GCCGGC	1 cut(s)	346
NarI	GGCGCC	1 cut(s)	348
NdeII	GATC	5 cut(s)	37, 139, 331, 624, 679
NgoMIV	GCCGGC	1 cut(s)	344
NlaIII	CATG	8 cut(s)	72, 187, 283, 485, 616, 692, 836, 858
NlaIV	GGNNCC	6 cut(s)	59, 74, 349, 456, 535, 765
NmuCI	GTSAC	1 cut(s)	646
PagI	TCATGA	1 cut(s)	854
PcsI	WCGNNNNNNNCGW	1 cut(s)	237
PdiI	GCCGGC	1 cut(s)	346
PfeI	GAWTC	3 cut(s)	485, 503, 794
PkrI	GCNGC	4 cut(s)	292, 343, 664, 848
PluTI	GGCGCC	1 cut(s)	351
PpuMI	RGGWCCY	1 cut(s)	455
Psp5II	RGGWCCY	1 cut(s)	455
PspEI	GGTNACC	1 cut(s)	270
PspN4I	GGNNCC	6 cut(s)	59, 74, 349, 456, 535, 765
PspOMI	GGGCCC	2 cut(s)	57, 72
PspPI	GGNCC	7 cut(s)	57, 58, 72, 73, 226, 455, 764
PspPPI	RGGWCCY	1 cut(s)	455
PstNI	CAGNNNCTG	2 cut(s)	650, 710
PsuI	RGATCY	2 cut(s)	37, 139
SacII	CCGCGG	1 cut(s)	168
SalI	GTCGAC	1 cut(s)	560
SaqAI	TTAA	2 cut(s)	393, 837
SatI	GCNGC	4 cut(s)	291, 342, 663, 847
Sau3AI	GATC	5 cut(s)	37, 139, 331, 624, 679
Sau96I	GGNCC	7 cut(s)	57, 58, 72, 73, 226, 455, 764
SduI	GDGCHC	4 cut(s)	61, 76, 254, 598
SfaNI	GCATC	4 cut(s)	126, 182, 484, 771
SfcI	CTRYAG	2 cut(s)	702, 802
SfoI	GGCGCC	1 cut(s)	349
Sfr303I	CCGCGG	1 cut(s)	168
SgrAI	CRCCGGYG	1 cut(s)	344
SgrBI	CCGCGG	1 cut(s)	168
SinI	GGWCC	3 cut(s)	226, 455, 764
SmlI	CTYRAG	1 cut(s)	392
SmoI	CTYRAG	1 cut(s)	392
SsiI	CCGC	9 cut(s)	104, 165, 167, 180, 261, 291, 342, 545, 778
SspDI	GGCGCC	1 cut(s)	347
StyI	CCWWGG	2 cut(s)	320, 462
TaaI	ACNGT	2 cut(s)	403, 476
TaiI	ACGT	1 cut(s)	249
TaqI	TCGA	5 cut(s)	91, 330, 561, 719, 810
TauI	GCSGC	2 cut(s)	293, 344
TfiI	GAWTC	3 cut(s)	485, 503, 794
Tru1I	TTAA	2 cut(s)	393, 837
Tru9I	TTAA	2 cut(s)	393, 837
TscAI	CASTG	4 cut(s)	119, 522, 535, 655
TseFI	GTSAC	1 cut(s)	646
TseI	GCWGC	2 cut(s)	662, 846
Tsp45I	GTSAC	1 cut(s)	646
TspDTI	ATGAA	5 cut(s)	296, 498, 762, 764, 828
TspRI	CASTG	4 cut(s)	119, 522, 535, 655
Vha464I	CTTAAG	1 cut(s)	392
VpaK11BI	GGWCC	3 cut(s)	226, 455, 764
XcmI	CCANNNNNNNNNTGG	1 cut(s)	448
XmiI	GTMKAC	1 cut(s)	561

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.