FvH4_4g05740

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0017137
Physical Location & Seq
Forward (+)
5120548 .. 5123070
2523 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g05740.t1

Sequence Viewer

Length: 1104 bp
ATGGCTATCACCCAAGAAATCCTCTATGTGCTTCTTACCATACTCAGCTCATCCTCTCTTTTTCTTCATCACCATTCGTGTAATGCGCAATCACTTGCATCATGCCAATTCGGTTCAATTTATCAATTTGGGGATTCATTTTCCGACACAGGAAACTTGGCTCTCGTGGCTCCGAAAGTACCCTGCAATCAACCTCCCTACGGGCAAAGTTTGCCCAATAAAGCAACCGGCCGATGCTCTGATGGGTTGTTGATAGTCGACTACATAGCACAAGCAGCGGGTCTATCTTTTATTGAACCATATTTCAGCAAAAATGCCACTCATAGCAGTGGAGTAAATTTCGCAATGGCTGGGGCTCCTGCTTCTTCTTTATATGAAAATCAAGATTACGGGCAACGTATACCGGCTATGGATCATGAACAACTGGATTTGTTTTCAAAATATTTGAATGGATCATCTTGTAGTCAAGATCGACTTAAAAGGTCTCTATTCATTGTGGGACAAATTGGACTCAATGATTACAATAATGGCTTATTACATGGAAATAAAAGTTTTGAGGAGGTAAAGAATATGGTTCTCAATGTAGTCCAAGCCATTAAGGACGCTGTTAAATGGGTGATTGGTTACGGTGCTGTAAAAGTCCTTGTCCCAGGACAATGGCCAATGGGTTGTTTGCCAATTTACCTTTCACGTTTTCCCACTAATGACACTGCTGCTTATGATCAATTTCATTGCCTAAAGGATTGGAATAGTATTTCAGATTGTCACAATTACCATCTGAGAAACGCCATTGAAGAACTGAAGAAAGAATACCCTGATGTTACTATCGTATATGCTGATTATTATAGCGCTTTTGAATGGATACTCACTCAGCAAGAGGAGTTAGGGTTTGAGGCCGTAGACAAGTTATGTTGTGGTGTTGAAGGTGAAGCGGACACTAGTGAAGTGAAAGCTTGTGGAATTTCTCCTGAGGTGCATGTGTGTGCCAATCCGGGTAATCTAATTAGTTGGGACGGGATTCATATGACACAACAAGCGCACAAGTATATAGCACAGAAACTCATTCGAGACATTTTACCTGATCTTAATTGTGGCCAAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00901 ko01110 map00901 map01110
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

368

Amino Acids

40.55

Weight (kDa)

5.22

Isoelectric Point (pI)

37.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 40 - 353 8.4e-30 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017137)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G03980
fragaria_vesca FvH4_4g05740
prunus_persica Prupe.4G256100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0363081
rosa_laevigata RLG00000027595
rosa_multiflora Rmu_sc0004472.1_g000006
rosa_roxburghii Rroxscaffold_4G00293480
rosa_rugosa Rorug01G0304500
rosa_samantha Rh1AG313500 Rh1BG276700 Rh1CG293400 Rh1DG307400
rosa_wichuraiana Rw1G027770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 87
AccI GTMKAC 3 cut(s) 258, 400, 900
AciI CCGC 2 cut(s) 278, 932
AclWI GGATC 2 cut(s) 420, 460
AcoI YGGCCR 3 cut(s) 229, 659, 1093
AcsI RAATTY 2 cut(s) 337, 960
AcuI CTGAAG 1 cut(s) 821
AfaI GTAC 1 cut(s) 180
AfeI AGCGCT 1 cut(s) 850
AfiI CCNNNNNNNGG 1 cut(s) 200
AgsI TTSAA 7 cut(s) 117, 296, 438, 448, 794, 857, 923
AhlI ACTAGT 1 cut(s) 938
AjnI CCWGG 1 cut(s) 649
AjuI GAANNNNNNNTTGG 2 cut(s) 670, 702
AluBI AGCT 2 cut(s) 48, 953
AluI AGCT 2 cut(s) 48, 953
Alw26I GTCTC 2 cut(s) 489, 1062
AlwI GGATC 2 cut(s) 420, 460
Aor51HI AGCGCT 1 cut(s) 850
AoxI GGCC 4 cut(s) 229, 659, 894, 1093
ApeKI GCWGC 2 cut(s) 275, 713
ApoI RAATTY 2 cut(s) 337, 960
AspLEI GCGC 3 cut(s) 88, 851, 1039
AsuC2I CCSGG 1 cut(s) 993
AsuHPI GGTGA 3 cut(s) 62, 628, 938
AxyI CCTNAGG 1 cut(s) 969
BalI TGGCCA 2 cut(s) 661, 1095
BanII GRGCYC 1 cut(s) 358
BarI GAAGNNNNNNTAC 2 cut(s) 794, 826
BauI CACGAG 1 cut(s) 164
BbvI GCAGC 2 cut(s) 287, 700
BccI CCATC 2 cut(s) 236, 783
BceAI ACGGC 1 cut(s) 881
BciT130I CCWGG 1 cut(s) 651
BciVI GTATCC 1 cut(s) 855
BclI TGATCA 1 cut(s) 721
BcnI CCSGG 1 cut(s) 993
BcoDI GTCTC 2 cut(s) 489, 1062
BcuI ACTAGT 1 cut(s) 938
BfaI CTAG 1 cut(s) 939
BfoI RGCGCY 1 cut(s) 852
BfuI GTATCC 1 cut(s) 855
BisI GCNGC 2 cut(s) 276, 714
BlsI GCNGC 2 cut(s) 277, 715
Bme1390I CCNGG 2 cut(s) 651, 993
BmiI GGNNCC 2 cut(s) 171, 357
BmrFI CCNGG 2 cut(s) 651, 993
BmsI GCATC 2 cut(s) 107, 224
BpuMI CCSGG 1 cut(s) 993
BsaI GGTCTC 1 cut(s) 489
BsaJI CCNNGG 1 cut(s) 649
BsaXI ACNNNNNCTCC 2 cut(s) 872, 902
Bsc4I CCNNNNNNNGG 1 cut(s) 200
Bse118I RCCGGY 2 cut(s) 227, 403
Bse1I ACTGG 1 cut(s) 429
Bse21I CCTNAGG 1 cut(s) 969
Bse3DI GCAATG 2 cut(s) 351, 730
BseBI CCWGG 1 cut(s) 651
BseDI CCNNGG 1 cut(s) 649
BseGI GGATG 1 cut(s) 50
BseLI CCNNNNNNNGG 1 cut(s) 200
BseMI GCAATG 2 cut(s) 351, 730
BseMII CTCAG 4 cut(s) 58, 770, 884, 960
BseNI ACTGG 1 cut(s) 429
BseRI GAGGAG 2 cut(s) 572, 893
BseX3I CGGCCG 1 cut(s) 229
BseXI GCAGC 2 cut(s) 287, 700
BseYI CCCAGC 1 cut(s) 350
Bsh1285I CGRYCG 1 cut(s) 232
BshFI GGCC 4 cut(s) 231, 661, 896, 1095
BsiEI CGRYCG 1 cut(s) 232
BsiSI CCGG 3 cut(s) 228, 404, 992
BslFI GGGAC 3 cut(s) 513, 632, 1025
BslI CCNNNNNNNGG 1 cut(s) 200
BsmAI GTCTC 2 cut(s) 489, 1062
BsmFI GGGAC 3 cut(s) 513, 632, 1025
BsnI GGCC 4 cut(s) 231, 661, 896, 1095
Bso31I GGTCTC 1 cut(s) 489
Bsp1286I GDGCHC 1 cut(s) 358
Bsp143I GATC 5 cut(s) 412, 452, 469, 721, 1081
BspACI CCGC 2 cut(s) 278, 932
BspANI GGCC 4 cut(s) 231, 661, 896, 1095
BspCNI CTCAG 4 cut(s) 57, 771, 883, 961
BspHI TCATGA 1 cut(s) 415
BspLI GGNNCC 2 cut(s) 171, 357
BspPI GGATC 2 cut(s) 420, 460
BspTNI GGTCTC 1 cut(s) 489
BsrDI GCAATG 2 cut(s) 351, 730
BsrFI RCCGGY 2 cut(s) 227, 403
BsrI ACTGG 1 cut(s) 429
BssAI RCCGGY 2 cut(s) 227, 403
BssECI CCNNGG 1 cut(s) 649
BssMI GATC 5 cut(s) 412, 452, 469, 721, 1081
BssNAI GTATAC 1 cut(s) 401
BssSI CACGAG 1 cut(s) 164
Bst1107I GTATAC 1 cut(s) 401
Bst2BI CACGAG 1 cut(s) 164
Bst2UI CCWGG 1 cut(s) 651
Bst4CI ACNGT 1 cut(s) 629
BstAPI GCANNNNNTGC 1 cut(s) 211
BstDEI CTNAG 4 cut(s) 44, 779, 870, 969
BstF5I GGATG 1 cut(s) 50
BstH2I RGCGCY 1 cut(s) 852
BstHHI GCGC 3 cut(s) 88, 851, 1039
BstKTI GATC 5 cut(s) 415, 455, 472, 724, 1084
BstMAI GTCTC 2 cut(s) 489, 1062
BstMBI GATC 5 cut(s) 412, 452, 469, 721, 1081
BstMCI CGRYCG 1 cut(s) 232
BstMWI GCNNNNNNNGC 3 cut(s) 167, 211, 275
BstNI CCWGG 1 cut(s) 651
BstNSI RCATGY 1 cut(s) 980
BstSCI CCNGG 2 cut(s) 649, 991
BstV1I GCAGC 2 cut(s) 287, 700
BstXI CCANNNNNNTGG 1 cut(s) 657
BstZ17I GTATAC 1 cut(s) 401
BstZI CGGCCG 1 cut(s) 229
Bsu36I CCTNAGG 1 cut(s) 969
BsuI GTATCC 1 cut(s) 855
BsuRI GGCC 4 cut(s) 231, 661, 896, 1095
BtsCI GGATG 1 cut(s) 50
BtsI GCAGTG 2 cut(s) 334, 708
BtsIMutI CAGTG 2 cut(s) 334, 708
CciI TCATGA 1 cut(s) 415
CfoI GCGC 3 cut(s) 88, 851, 1039
Cfr10I RCCGGY 2 cut(s) 227, 403
CseI GACGC 1 cut(s) 611
Csp6I GTAC 1 cut(s) 179
CviAII CATG 4 cut(s) 102, 416, 539, 977
CviQI GTAC 1 cut(s) 179
DdeI CTNAG 4 cut(s) 44, 779, 870, 969
DpnI GATC 5 cut(s) 414, 454, 471, 723, 1083
DpnII GATC 5 cut(s) 412, 452, 469, 721, 1081
EaeI YGGCCR 3 cut(s) 229, 659, 1093
EagI CGGCCG 1 cut(s) 229
EclXI CGGCCG 1 cut(s) 229
Eco24I GRGCYC 1 cut(s) 358
Eco31I GGTCTC 1 cut(s) 489
Eco47III AGCGCT 1 cut(s) 850
Eco52I CGGCCG 1 cut(s) 229
Eco57I CTGAAG 1 cut(s) 821
Eco81I CCTNAGG 1 cut(s) 969
EcoRII CCWGG 1 cut(s) 649
EcoT38I GRGCYC 1 cut(s) 358
FaeI CATG 4 cut(s) 105, 419, 542, 980
FalI AAGNNNNNCTT 2 cut(s) 459, 491
FaqI GGGAC 3 cut(s) 513, 632, 1025
FatI CATG 4 cut(s) 101, 415, 538, 976
FauI CCCGC 1 cut(s) 271
FauNDI CATATG 1 cut(s) 1023
FbaI TGATCA 1 cut(s) 721
FblI GTMKAC 3 cut(s) 258, 400, 900
Fnu4HI GCNGC 2 cut(s) 276, 714
FokI GGATG 1 cut(s) 37
FriOI GRGCYC 1 cut(s) 358
Fsp4HI GCNGC 2 cut(s) 276, 714
FspBI CTAG 1 cut(s) 939
FspI TGCGCA 1 cut(s) 87
GlaI GCGC 3 cut(s) 87, 850, 1038
GluI GCNGC 2 cut(s) 276, 714
GsaI CCCAGC 1 cut(s) 354
HaeII RGCGCY 1 cut(s) 852
HaeIII GGCC 4 cut(s) 231, 661, 896, 1095
HapII CCGG 3 cut(s) 228, 404, 992
HgaI GACGC 1 cut(s) 611
HhaI GCGC 3 cut(s) 88, 851, 1039
Hin1II CATG 4 cut(s) 105, 419, 542, 980
Hin6I GCGC 3 cut(s) 86, 849, 1037
HinP1I GCGC 3 cut(s) 86, 849, 1037
HincII GTYRAC 1 cut(s) 259
HindII GTYRAC 1 cut(s) 259
HindIII AAGCTT 1 cut(s) 951
HinfI GANTC 3 cut(s) 134, 510, 1018
HpaII CCGG 3 cut(s) 228, 404, 992
HphI GGTGA 3 cut(s) 62, 628, 938
Hpy166II GTNNAC 3 cut(s) 259, 401, 901
Hpy188I TCNGA 5 cut(s) 145, 174, 241, 760, 780
Hpy188III TCNNGA 5 cut(s) 383, 416, 467, 968, 1067
Hpy8I GTNNAC 3 cut(s) 259, 401, 901
HpyAV CCTTC 1 cut(s) 917
HpyCH4III ACNGT 1 cut(s) 629
HpyCH4IV ACGT 2 cut(s) 397, 691
HpyCH4V TGCA 3 cut(s) 98, 186, 976
HpyF10VI GCNNNNNNNGC 3 cut(s) 167, 211, 275
HpyF3I CTNAG 4 cut(s) 44, 779, 870, 969
HpySE526I ACGT 2 cut(s) 397, 691
Hsp92II CATG 4 cut(s) 105, 419, 542, 980
HspAI GCGC 3 cut(s) 86, 849, 1037
Ksp22I TGATCA 1 cut(s) 721
Kzo9I GATC 5 cut(s) 412, 452, 469, 721, 1081
LmnI GCTCC 2 cut(s) 175, 361
Lsp1109I GCAGC 2 cut(s) 287, 700
LweI GCATC 2 cut(s) 107, 224
MaeI CTAG 1 cut(s) 939
MaeII ACGT 2 cut(s) 397, 691
MaeIII GTNAC 3 cut(s) 623, 764, 820
MalI GATC 5 cut(s) 414, 454, 471, 723, 1083
MboI GATC 5 cut(s) 412, 452, 469, 721, 1081
MboII GAAGA 4 cut(s) 56, 357, 806, 814
MhlI GDGCHC 1 cut(s) 358
MlsI TGGCCA 2 cut(s) 661, 1095
MluNI TGGCCA 2 cut(s) 661, 1095
MlyI GAGTC 1 cut(s) 504
MmeI TCCRAC 1 cut(s) 168
MnlI CCTC 8 cut(s) 32, 64, 204, 550, 553, 871, 886, 964
Mox20I TGGCCA 2 cut(s) 661, 1095
MscI TGGCCA 2 cut(s) 661, 1095
MseI TTAA 4 cut(s) 477, 597, 609, 1086
MslI CAYNNNNRTG 2 cut(s) 327, 981
Msp20I TGGCCA 2 cut(s) 661, 1095
MspA1I CMGCKG 1 cut(s) 278
MspI CCGG 3 cut(s) 228, 404, 992
MspR9I CCNGG 2 cut(s) 651, 993
MvaI CCWGG 1 cut(s) 651
MwoI GCNNNNNNNGC 3 cut(s) 167, 211, 275
NciI CCSGG 1 cut(s) 993
NdeI CATATG 1 cut(s) 1023
NdeII GATC 5 cut(s) 412, 452, 469, 721, 1081
NlaIII CATG 4 cut(s) 105, 419, 542, 980
NlaIV GGNNCC 2 cut(s) 171, 357
NmuCI GTSAC 1 cut(s) 764
NsbI TGCGCA 1 cut(s) 87
NspI RCATGY 1 cut(s) 980
PagI TCATGA 1 cut(s) 415
PfeI GAWTC 2 cut(s) 134, 1018
PkrI GCNGC 2 cut(s) 277, 715
PleI GAGTC 1 cut(s) 504
PpsI GAGTC 1 cut(s) 504
Psp6I CCWGG 1 cut(s) 649
PspFI CCCAGC 1 cut(s) 350
PspGI CCWGG 1 cut(s) 649
PspN4I GGNNCC 2 cut(s) 171, 357
RsaI GTAC 1 cut(s) 180
RsaNI GTAC 1 cut(s) 179
RseI CAYNNNNRTG 2 cut(s) 327, 981
SalI GTCGAC 1 cut(s) 257
SaqAI TTAA 4 cut(s) 477, 597, 609, 1086
SatI GCNGC 2 cut(s) 276, 714
Sau3AI GATC 5 cut(s) 412, 452, 469, 721, 1081
SchI GAGTC 1 cut(s) 504
ScrFI CCNGG 2 cut(s) 651, 993
SduI GDGCHC 1 cut(s) 358
SfaNI GCATC 2 cut(s) 107, 224
SmiMI CAYNNNNRTG 2 cut(s) 327, 981
SpeI ACTAGT 1 cut(s) 938
SsiI CCGC 2 cut(s) 278, 932
SspI AATATT 1 cut(s) 443
SspMI CTAG 1 cut(s) 939
StyD4I CCNGG 2 cut(s) 649, 991
TaaI ACNGT 1 cut(s) 629
TaiI ACGT 2 cut(s) 400, 694
TaqI TCGA 3 cut(s) 258, 472, 1066
TfiI GAWTC 2 cut(s) 134, 1018
Tru1I TTAA 4 cut(s) 477, 597, 609, 1086
Tru9I TTAA 4 cut(s) 477, 597, 609, 1086
TscAI CASTG 2 cut(s) 334, 715
TseFI GTSAC 1 cut(s) 764
TseI GCWGC 2 cut(s) 275, 713
Tsp45I GTSAC 1 cut(s) 764
TspDTI ATGAA 7 cut(s) 56, 126, 390, 432, 481, 719, 1010
TspRI CASTG 2 cut(s) 334, 715
XapI RAATTY 2 cut(s) 337, 960
XceI RCATGY 1 cut(s) 980
XmiI GTMKAC 3 cut(s) 258, 400, 900
XspI CTAG 1 cut(s) 939
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.