Rh1AG313500

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0017137
Physical Location & Seq
Forward (+)
54617390 .. 54619476
2087 bp
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UTR
Exon/CDS
Intron
Rh1AG313500.1

Sequence Viewer

Length: 1170 bp
ATGATTTGTGGCATAACTCAAAACAAAGCATTGTTTCTCCTCTCCTCGATGGCAACCAAAGCAAGCCTATTTTTCATGCTTAGCTCCTTGTTTCTTCTTTGGAAGGTTAACTCCTCATTTCTTCTTCGGAATTTAAGCAATGCAGCATCATTGAAAGCTTGTAACTTGGAAGCCATATATCAATTTGGGGACTCTTTCTCCGACACCGGAAATGCGATACGTGAAAACAAAGCTCTAGAGGCTACTAAGCTCCCTTATGGCTTAAATCTTCCCAGGGAACCAAAAGGTCGATTTTCCGACGGGATGTTGGTTATAGATTTTGAAGCACAAGCAGCTGAGCTTCCATTTCTTAAACCATATTTGGATCAGGAAGCGAAAGATTACCACAACGGAAAGAACTTTGCTGTTAGTTCTGCTAGCGTTTTGCCAAAAGGTGTTCTTAAAGAGAAATCAATACTCTTCCCAGAAACAATGACAAACATTACGCTACATAAGCAAGTGGATTGGATGGCCACTTACTTCAATGAAACCCCCTATTTTAGTCAAGACCGCGCAGAGAAGCTTAAGAGGTCACTCTTTTTCATCGGACCAATGGCACATGGTGATTATATGTATTCAATGGACTTGAGTCGTAAATCTTCAGAATGGGTAAAGAGCAATTTGGTTCCAGATATCGTCCAAACCATCAAAGAAGCTGCCACAAGGGTCATTGCTTTTGGTGCTGTGAAAGTGGTCATCCCAGGAATATACGCATTTGGTTGTTTTAAAGCTCCAGGTTATAATGTATCGCTATGCGACCAGGGCAAAAATAGTGTTGCAACCACTCACAACGATCTGCTTCGGAAAGCAATTGAGGAGTTAAAGAAACAACACCCTAATGTGACTATTGTATATGCAGATTATTACAGTGCATTCATGTCCTTCTTCCCCAAGGCACTTAGTCTAGGGTTTGAACCTGCGAACAAGCCATGCTGTTTCGGCACACATAAAAATGGTCCAGGACATGGTTTTAACCCGGATAACATGTGTGGAAAGCCTGGAGTACAAGCTTGTGCTAACCCTGCGAAACGAATCAACTGGGATGGTAGTCATATGACGCAACATGCATACAAGTTAATAGCAGATTTGATAATTCCTGACATCGCAGCCAAGCTTAAATGCAGTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00901 ko01110 map00901 map01110
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

389

Amino Acids

43.13

Weight (kDa)

8.94

Isoelectric Point (pI)

30.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 59 - 377 2.6e-22 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017137)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G03980
fragaria_vesca FvH4_4g05740
prunus_persica Prupe.4G256100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0363081
rosa_laevigata RLG00000027595
rosa_multiflora Rmu_sc0004472.1_g000006
rosa_roxburghii Rroxscaffold_4G00293480
rosa_rugosa Rorug01G0304500
rosa_samantha Rh1AG313500 Rh1BG276700 Rh1CG293400 Rh1DG307400
rosa_wichuraiana Rw1G027770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 780
Acc36I ACCTGC 1 cut(s) 964
AccB7I CCANNNNNTGG 1 cut(s) 1004
AccII CGCG 1 cut(s) 552
AciI CCGC 1 cut(s) 550
AclWI GGATC 1 cut(s) 372
AcoI YGGCCR 1 cut(s) 510
AcsI RAATTY 1 cut(s) 130
AcuI CTGAAG 1 cut(s) 624
AdeI CACNNNGTG 1 cut(s) 602
AfaI GTAC 1 cut(s) 1044
AfiI CCNNNNNNNGG 1 cut(s) 1004
AflII CTTAAG 1 cut(s) 563
AflIII ACRYGT 1 cut(s) 1023
AgsI TTSAA 5 cut(s) 154, 323, 523, 618, 953
AjnI CCWGG 6 cut(s) 272, 739, 772, 798, 997, 1036
AlwI GGATC 1 cut(s) 372
AoxI GGCC 1 cut(s) 510
ApeKI GCWGC 4 cut(s) 143, 332, 695, 1145
ApoI RAATTY 1 cut(s) 130
AspLEI GCGC 1 cut(s) 554
AspS9I GGNCC 2 cut(s) 587, 995
AsuC2I CCSGG 1 cut(s) 1016
AsuHPI GGTGA 1 cut(s) 614
AsuNHI GCTAGC 1 cut(s) 416
AvaII GGWCC 2 cut(s) 587, 995
BalI TGGCCA 1 cut(s) 512
BbvI GCAGC 4 cut(s) 155, 344, 682, 1157
BccI CCATC 4 cut(s) 43, 502, 692, 1076
BciT130I CCWGG 6 cut(s) 274, 741, 774, 800, 999, 1038
BcnI CCSGG 1 cut(s) 1016
BfaI CTAG 3 cut(s) 236, 417, 944
BfrI CTTAAG 1 cut(s) 563
BfuAI ACCTGC 1 cut(s) 964
BisI GCNGC 4 cut(s) 144, 333, 696, 1146
BlpI GCTNAGC 2 cut(s) 80, 336
BlsI GCNGC 4 cut(s) 145, 334, 697, 1147
Bme1390I CCNGG 7 cut(s) 274, 741, 774, 800, 999, 1016, 1038
Bme18I GGWCC 2 cut(s) 587, 995
BmgT120I GGNCC 2 cut(s) 587, 995
BmiI GGNNCC 2 cut(s) 279, 666
BmrFI CCNGG 7 cut(s) 274, 741, 774, 800, 999, 1016, 1038
BmrI ACTGGG 1 cut(s) 1087
BmsI GCATC 1 cut(s) 155
BmtI GCTAGC 1 cut(s) 420
BmuI ACTGGG 1 cut(s) 1087
BoxI GACNNNNGTC 1 cut(s) 627
BpmI CTGGAG 2 cut(s) 756, 1059
Bpu1102I GCTNAGC 2 cut(s) 80, 336
BpuEI CTTGAG 1 cut(s) 646
BpuMI CCSGG 1 cut(s) 1016
BsaAI YACGTR 1 cut(s) 221
BsaJI CCNNGG 5 cut(s) 272, 273, 739, 799, 930
BsaWI WCCGGW 1 cut(s) 206
BsaXI ACNNNNNCTCC 2 cut(s) 182, 212
Bsc4I CCNNNNNNNGG 1 cut(s) 1004
Bse1I ACTGG 1 cut(s) 1082
Bse3DI GCAATG 2 cut(s) 145, 708
BseBI CCWGG 6 cut(s) 274, 741, 774, 800, 999, 1038
BseDI CCNNGG 5 cut(s) 272, 273, 739, 799, 930
BseGI GGATG 4 cut(s) 309, 513, 735, 1087
BseLI CCNNNNNNNGG 1 cut(s) 1004
BseMI GCAATG 2 cut(s) 145, 708
BseMII CTCAG 1 cut(s) 327
BseNI ACTGG 1 cut(s) 1082
BseRI GAGGAG 4 cut(s) 29, 34, 103, 869
BseXI GCAGC 4 cut(s) 155, 344, 682, 1157
Bsh1236I CGCG 1 cut(s) 552
BshFI GGCC 1 cut(s) 512
BsiSI CCGG 2 cut(s) 207, 1016
BslFI GGGAC 1 cut(s) 203
BslI CCNNNNNNNGG 1 cut(s) 1004
BsmFI GGGAC 1 cut(s) 203
BsmI GAATGC 1 cut(s) 911
BsnI GGCC 1 cut(s) 512
Bsp143I GATC 2 cut(s) 364, 832
Bsp1720I GCTNAGC 2 cut(s) 80, 336
BspACI CCGC 1 cut(s) 550
BspANI GGCC 1 cut(s) 512
BspCNI CTCAG 1 cut(s) 328
BspFNI CGCG 1 cut(s) 552
BspLI GGNNCC 2 cut(s) 279, 666
BspMI ACCTGC 1 cut(s) 964
BspOI GCTAGC 1 cut(s) 420
BspPI GGATC 1 cut(s) 372
BspTI CTTAAG 1 cut(s) 563
BsrDI GCAATG 2 cut(s) 145, 708
BsrI ACTGG 1 cut(s) 1082
BssECI CCNNGG 5 cut(s) 272, 273, 739, 799, 930
BssMI GATC 2 cut(s) 364, 832
BssT1I CCWWGG 1 cut(s) 930
Bst2UI CCWGG 6 cut(s) 274, 741, 774, 800, 999, 1038
Bst4CI ACNGT 1 cut(s) 908
Bst6I CTCTTC 1 cut(s) 464
BstAFI CTTAAG 1 cut(s) 563
BstBAI YACGTR 1 cut(s) 221
BstC8I GCNNGC 2 cut(s) 64, 418
BstDEI CTNAG 4 cut(s) 80, 246, 336, 938
BstF5I GGATG 4 cut(s) 309, 513, 735, 1087
BstFNI CGCG 1 cut(s) 552
BstHHI GCGC 1 cut(s) 554
BstKTI GATC 2 cut(s) 367, 835
BstMBI GATC 2 cut(s) 364, 832
BstMWI GCNNNNNNNGC 8 cut(s) 59, 239, 332, 493, 719, 801, 978, 1061
BstNI CCWGG 6 cut(s) 274, 741, 774, 800, 999, 1038
BstNSI RCATGY 2 cut(s) 1027, 1106
BstPAI GACNNNNGTC 1 cut(s) 627
BstSCI CCNGG 7 cut(s) 272, 739, 772, 798, 997, 1014, 1036
BstUI CGCG 1 cut(s) 552
BstV1I GCAGC 4 cut(s) 155, 344, 682, 1157
BsuRI GGCC 1 cut(s) 512
BtgZI GCGATG 1 cut(s) 1126
BtsCI GGATG 4 cut(s) 309, 513, 735, 1087
BtsI GCAGTG 1 cut(s) 1168
BtsIMutI CAGTG 2 cut(s) 913, 1168
BveI ACCTGC 1 cut(s) 964
Cac8I GCNNGC 2 cut(s) 64, 418
CfoI GCGC 1 cut(s) 554
Cfr13I GGNCC 2 cut(s) 587, 995
CseI GACGC 1 cut(s) 1105
Csp6I GTAC 1 cut(s) 1043
CviAII CATG 7 cut(s) 76, 599, 916, 969, 1004, 1024, 1103
CviQI GTAC 1 cut(s) 1043
DdeI CTNAG 4 cut(s) 80, 246, 336, 938
DpnI GATC 2 cut(s) 366, 834
DpnII GATC 2 cut(s) 364, 832
DraI TTTAAA 1 cut(s) 766
DraIII CACNNNGTG 1 cut(s) 602
EaeI YGGCCR 1 cut(s) 510
Eam1104I CTCTTC 1 cut(s) 464
EarI CTCTTC 1 cut(s) 464
Eco130I CCWWGG 1 cut(s) 930
Eco32I GATATC 1 cut(s) 673
Eco47I GGWCC 2 cut(s) 587, 995
Eco57I CTGAAG 1 cut(s) 624
EcoRII CCWGG 6 cut(s) 272, 739, 772, 798, 997, 1036
EcoRV GATATC 1 cut(s) 673
EcoT14I CCWWGG 1 cut(s) 930
EcoT22I ATGCAT 1 cut(s) 1108
ErhI CCWWGG 1 cut(s) 930
FaeI CATG 7 cut(s) 79, 602, 919, 972, 1007, 1027, 1106
FalI AAGNNNNNCTT 2 cut(s) 423, 455
FaqI GGGAC 1 cut(s) 203
FatI CATG 7 cut(s) 75, 598, 915, 968, 1003, 1023, 1102
FauNDI CATATG 1 cut(s) 1092
Fnu4HI GCNGC 4 cut(s) 144, 333, 696, 1146
FokI GGATG 4 cut(s) 316, 520, 722, 1094
Fsp4HI GCNGC 4 cut(s) 144, 333, 696, 1146
FspBI CTAG 3 cut(s) 236, 417, 944
GlaI GCGC 1 cut(s) 553
GluI GCNGC 4 cut(s) 144, 333, 696, 1146
GsuI CTGGAG 2 cut(s) 756, 1059
HaeIII GGCC 1 cut(s) 512
HapII CCGG 2 cut(s) 207, 1016
HgaI GACGC 1 cut(s) 1105
HhaI GCGC 1 cut(s) 554
Hin1II CATG 7 cut(s) 79, 602, 919, 972, 1007, 1027, 1106
Hin6I GCGC 1 cut(s) 552
HinP1I GCGC 1 cut(s) 552
HincII GTYRAC 1 cut(s) 109
HindII GTYRAC 1 cut(s) 109
HindIII AAGCTT 4 cut(s) 156, 560, 1047, 1151
HinfI GANTC 3 cut(s) 191, 628, 1071
HpaI GTTAAC 1 cut(s) 109
HpaII CCGG 2 cut(s) 207, 1016
HphI GGTGA 1 cut(s) 614
Hpy166II GTNNAC 1 cut(s) 109
Hpy188I TCNGA 6 cut(s) 129, 202, 298, 587, 643, 843
Hpy188III TCNNGA 5 cut(s) 236, 368, 545, 668, 1136
Hpy8I GTNNAC 1 cut(s) 109
Hpy99I CGWCG 1 cut(s) 302
HpyAV CCTTC 2 cut(s) 97, 931
HpyCH4III ACNGT 1 cut(s) 908
HpyCH4IV ACGT 1 cut(s) 220
HpyCH4V TGCA 7 cut(s) 143, 818, 896, 911, 1106, 1161, 1166
HpyF10VI GCNNNNNNNGC 8 cut(s) 59, 239, 332, 493, 719, 801, 978, 1061
HpyF3I CTNAG 4 cut(s) 80, 246, 336, 938
HpySE526I ACGT 1 cut(s) 220
Hsp92II CATG 7 cut(s) 79, 602, 919, 972, 1007, 1027, 1106
HspAI GCGC 1 cut(s) 552
KspAI GTTAAC 1 cut(s) 109
Kzo9I GATC 2 cut(s) 364, 832
LmnI GCTCC 3 cut(s) 89, 255, 775
Lsp1109I GCAGC 4 cut(s) 155, 344, 682, 1157
LweI GCATC 1 cut(s) 155
MaeI CTAG 3 cut(s) 236, 417, 944
MaeII ACGT 1 cut(s) 220
MaeIII GTNAC 3 cut(s) 161, 570, 880
MalI GATC 2 cut(s) 366, 834
MboI GATC 2 cut(s) 364, 832
MboII GAAGA 7 cut(s) 86, 113, 116, 260, 451, 630, 916
MfeI CAATTG 1 cut(s) 849
MlsI TGGCCA 1 cut(s) 512
MluCI AATT 5 cut(s) 130, 182, 658, 849, 1131
MluNI TGGCCA 1 cut(s) 512
MlyI GAGTC 2 cut(s) 185, 637
MmeI TCCRAC 2 cut(s) 225, 321
MnlI CCTC 6 cut(s) 50, 55, 124, 232, 561, 847
Mox20I TGGCCA 1 cut(s) 512
Mph1103I ATGCAT 1 cut(s) 1108
MscI TGGCCA 1 cut(s) 512
MslI CAYNNNNRTG 2 cut(s) 876, 990
Msp20I TGGCCA 1 cut(s) 512
MspA1I CMGCKG 1 cut(s) 335
MspCI CTTAAG 1 cut(s) 563
MspI CCGG 2 cut(s) 207, 1016
MspR9I CCNGG 7 cut(s) 274, 741, 774, 800, 999, 1016, 1038
MunI CAATTG 1 cut(s) 849
Mva1269I GAATGC 1 cut(s) 911
MvaI CCWGG 6 cut(s) 274, 741, 774, 800, 999, 1038
MvnI CGCG 1 cut(s) 552
MwoI GCNNNNNNNGC 8 cut(s) 59, 239, 332, 493, 719, 801, 978, 1061
NciI CCSGG 1 cut(s) 1016
NdeI CATATG 1 cut(s) 1092
NdeII GATC 2 cut(s) 364, 832
NheI GCTAGC 1 cut(s) 416
NlaIII CATG 7 cut(s) 79, 602, 919, 972, 1007, 1027, 1106
NlaIV GGNNCC 2 cut(s) 279, 666
NmuCI GTSAC 2 cut(s) 570, 880
NsiI ATGCAT 1 cut(s) 1108
NspI RCATGY 2 cut(s) 1027, 1106
PasI CCCWGGG 1 cut(s) 273
PciI ACATGT 1 cut(s) 1023
PctI GAATGC 1 cut(s) 911
PfeI GAWTC 1 cut(s) 1071
PflMI CCANNNNNTGG 1 cut(s) 1004
PfoI TCCNGGA 1 cut(s) 997
PkrI GCNGC 4 cut(s) 145, 334, 697, 1147
PleI GAGTC 2 cut(s) 185, 636
PpsI GAGTC 2 cut(s) 185, 636
Ppu21I YACGTR 1 cut(s) 221
PscI ACATGT 1 cut(s) 1023
PshAI GACNNNNGTC 1 cut(s) 627
PsiI TTATAA 1 cut(s) 780
Psp6I CCWGG 6 cut(s) 272, 739, 772, 798, 997, 1036
PspGI CCWGG 6 cut(s) 272, 739, 772, 798, 997, 1036
PspN4I GGNNCC 2 cut(s) 279, 666
PspPI GGNCC 2 cut(s) 587, 995
PvuII CAGCTG 1 cut(s) 335
RsaI GTAC 1 cut(s) 1044
RsaNI GTAC 1 cut(s) 1043
RseI CAYNNNNRTG 2 cut(s) 876, 990
SatI GCNGC 4 cut(s) 144, 333, 696, 1146
Sau3AI GATC 2 cut(s) 364, 832
Sau96I GGNCC 2 cut(s) 587, 995
SchI GAGTC 2 cut(s) 185, 637
ScrFI CCNGG 7 cut(s) 274, 741, 774, 800, 999, 1016, 1038
SfaNI GCATC 1 cut(s) 155
SinI GGWCC 2 cut(s) 587, 995
SmiMI CAYNNNNRTG 2 cut(s) 876, 990
SmlI CTYRAG 2 cut(s) 563, 625
SmoI CTYRAG 2 cut(s) 563, 625
Sse9I AATT 5 cut(s) 130, 182, 658, 849, 1131
SsiI CCGC 1 cut(s) 550
SspMI CTAG 3 cut(s) 236, 417, 944
StyD4I CCNGG 7 cut(s) 272, 739, 772, 798, 997, 1014, 1036
StyI CCWWGG 1 cut(s) 930
TaaI ACNGT 1 cut(s) 908
TaiI ACGT 1 cut(s) 223
TaqI TCGA 2 cut(s) 47, 289
TasI AATT 5 cut(s) 130, 182, 658, 849, 1131
TatI WGTACW 1 cut(s) 1042
TfiI GAWTC 1 cut(s) 1071
TscAI CASTG 2 cut(s) 913, 1168
TseFI GTSAC 2 cut(s) 570, 880
TseI GCWGC 4 cut(s) 143, 332, 695, 1145
Tsp45I GTSAC 2 cut(s) 570, 880
TspDTI ATGAA 4 cut(s) 64, 540, 571, 904
TspGWI ACGGA 1 cut(s) 405
TspRI CASTG 2 cut(s) 913, 1168
Van91I CCANNNNNTGG 1 cut(s) 1004
Vha464I CTTAAG 1 cut(s) 563
VpaK11BI GGWCC 2 cut(s) 587, 995
XapI RAATTY 1 cut(s) 130
XbaI TCTAGA 1 cut(s) 235
XceI RCATGY 2 cut(s) 1027, 1106
XspI CTAG 3 cut(s) 236, 417, 944
Zsp2I ATGCAT 1 cut(s) 1108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.