Prupe.4G256100_v2.0.a1

Acetylajmalan esterase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0017137
Physical Location & Seq
Forward (+)
17946876 .. 17950522
3647 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G256100.1

Sequence Viewer

Length: 1107 bp
ATGGCTACCACCCAAGAAAACATCTATGTCCTGCTCATCATCAGCTCCTTGTCTCTTTTTCTTCATCAACATTCATGCAGTGCACACTCGCTTATGCAATGCAGATTGAAGGCAATATATCAACTGGGTGACTCGATTTCGGACACCGGAAATTTGGCTCGTTTAGCCCCTTCTATGACCTGTAATAAGCCCCCTTTTGGGCGAAGTTTCTTCAAAGCAACAGGCAGATGCTCTAATGGAATGCTCATAATTGATTACGTTGCTCAAGCAGCTAGTCTTCCCTTTCTCGAACCCTATATGAAGAAAGAAGCCACTCCCCGTAGTGGAGTGAATTTTGCCACGGCTGGGGCTACTGCTTTGCCTTCGAATGTTCGTGTACATTCCTTGAGTGAACAACTAGTTTGGTTGTCCACATATTTCAATGGAAGCAATGCAGATCGTTTTAAGAAGCTTGAGAGGGCTTTGTTCATAGTGGGAGAAATTGGACTAAATGACTACATTGCTTCCCTGAATGTGAAAAGTATTGAGGATATAAGGCATAACATGGTTCCGGAAATAGTCCAAGCCACCATGGATGCTGTTAAAATGGTCATTGGTTATGGTGCTAGGGCAGTGGTAGTCCCAGGACAATCCCCAATGGGGTGTTTGCCAATGCTCCTTTCGCGTTTACGCACTAATGATACAGCATCGTACGATGAGTTTCAATGCCTAATGGGGTTGAATAGTATTTCAAAGTCTCACAATCACCTTCTGAAACAAGCCATTGAAGCCTTGAAAAAGGAGTACCCCAATGTTTCCTTCATATACAGTGATTATTATGATGCTTTCACATGGTTACTTCGTAATGCACATCAGCTAGGGTTTGATGCTACGGACAAGTCATGTTGTGGTGGTGAGGACAGAGCCTGTGGAGCTCCTAATGCACCAGTTTGTGGTAATCCGGATAGGAGAATTAGTTGGGATGGTGTTCATATGACACAAAAGGCTAACAAAGATGGAGCTGAAAGCAACGTCGCATTGCATGCCAAAATACAGCTCCTCCTCCTACCGCACTGTGCGCGTTTTATATGGATTTATGAATGGAAGGAAGGAGAGGAAGAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00901 ko01110 map00901 map01110
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

369

Amino Acids

40.74

Weight (kDa)

7.14

Isoelectric Point (pI)

38.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017137)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G03980
fragaria_vesca FvH4_4g05740
prunus_persica Prupe.4G256100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0363081
rosa_laevigata RLG00000027595
rosa_multiflora Rmu_sc0004472.1_g000006
rosa_roxburghii Rroxscaffold_4G00293480
rosa_rugosa Rorug01G0304500
rosa_samantha Rh1AG313500 Rh1BG276700 Rh1CG293400 Rh1DG307400
rosa_wichuraiana Rw1G027770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 932
AccII CGCG 2 cut(s) 664, 1060
AccIII TCCGGA 2 cut(s) 550, 940
AciI CCGC 1 cut(s) 1049
AcsI RAATTY 2 cut(s) 151, 331
AfaI GTAC 3 cut(s) 378, 692, 785
AfiI CCNNNNNNNGG 6 cut(s) 197, 198, 323, 345, 639, 932
AgsI TTSAA 8 cut(s) 109, 214, 421, 704, 721, 732, 767, 775
AhlI ACTAGT 1 cut(s) 397
AjnI CCWGG 1 cut(s) 622
AjuI GAANNNNNNNTTGG 4 cut(s) 643, 675, 782, 814
AluBI AGCT 8 cut(s) 45, 272, 451, 856, 914, 1001, 1036, 1103
AluI AGCT 8 cut(s) 45, 272, 451, 856, 914, 1001, 1036, 1103
Alw21I GWGCWC 2 cut(s) 85, 916
Alw26I GTCTC 2 cut(s) 57, 741
Alw44I GTGCAC 1 cut(s) 81
AlwNI CAGNNNCTG 1 cut(s) 906
Aor13HI TCCGGA 2 cut(s) 550, 940
ApaLI GTGCAC 1 cut(s) 81
ApeKI GCWGC 1 cut(s) 269
ApoI RAATTY 2 cut(s) 151, 331
AspLEI GCGC 1 cut(s) 1060
AsuHPI GGTGA 3 cut(s) 140, 737, 905
AsuII TTCGAA 1 cut(s) 365
BaeGI GKGCMC 1 cut(s) 85
BanII GRGCYC 1 cut(s) 916
BbsI GAAGAC 1 cut(s) 269
Bbv12I GWGCWC 2 cut(s) 85, 916
BbvI GCAGC 1 cut(s) 281
BccI CCATC 2 cut(s) 956, 989
BceAI ACGGC 1 cut(s) 357
BciT130I CCWGG 1 cut(s) 624
BcoDI GTCTC 2 cut(s) 57, 741
BcuI ACTAGT 1 cut(s) 397
BfaI CTAG 4 cut(s) 273, 398, 606, 857
BisI GCNGC 1 cut(s) 270
BlsI GCNGC 1 cut(s) 271
Bme1390I CCNGG 1 cut(s) 624
BmiI GGNNCC 1 cut(s) 549
BmrFI CCNGG 1 cut(s) 624
BmrI ACTGGG 1 cut(s) 134
BmsI GCATC 5 cut(s) 218, 565, 695, 811, 856
BmuI ACTGGG 1 cut(s) 134
BpiI GAAGAC 1 cut(s) 269
Bpu14I TTCGAA 1 cut(s) 365
BpuEI CTTGAG 3 cut(s) 249, 406, 473
BsaJI CCNNGG 3 cut(s) 339, 570, 622
BsaWI WCCGGW 3 cut(s) 146, 550, 940
BsaXI ACNNNNNCTCC 4 cut(s) 940, 970, 1023, 1053
Bsc4I CCNNNNNNNGG 6 cut(s) 197, 198, 323, 345, 639, 932
Bse1I ACTGG 2 cut(s) 129, 926
Bse3DI GCAATG 4 cut(s) 104, 436, 498, 1016
BseAI TCCGGA 2 cut(s) 550, 940
BseBI CCWGG 1 cut(s) 624
BseDI CCNNGG 3 cut(s) 339, 570, 622
BseGI GGATG 2 cut(s) 580, 967
BseLI CCNNNNNNNGG 6 cut(s) 197, 198, 323, 345, 639, 932
BseMI GCAATG 4 cut(s) 104, 436, 498, 1016
BseNI ACTGG 2 cut(s) 129, 926
BseRI GAGGAG 2 cut(s) 1028, 1031
BseSI GKGCMC 1 cut(s) 85
BseXI GCAGC 1 cut(s) 281
BseYI CCCAGC 1 cut(s) 344
Bsh1236I CGCG 2 cut(s) 664, 1060
BsiHKAI GWGCWC 2 cut(s) 85, 916
BsiSI CCGG 3 cut(s) 147, 551, 941
BsiWI CGTACG 1 cut(s) 690
BslFI GGGAC 1 cut(s) 605
BslI CCNNNNNNNGG 6 cut(s) 197, 198, 323, 345, 639, 932
BsmAI GTCTC 2 cut(s) 57, 741
BsmFI GGGAC 1 cut(s) 605
BsmI GAATGC 1 cut(s) 246
Bsp119I TTCGAA 1 cut(s) 365
Bsp1286I GDGCHC 2 cut(s) 85, 916
Bsp13I TCCGGA 2 cut(s) 550, 940
Bsp1407I TGTACA 1 cut(s) 376
Bsp143I GATC 1 cut(s) 436
Bsp19I CCATGG 1 cut(s) 570
BspACI CCGC 1 cut(s) 1049
BspEI TCCGGA 2 cut(s) 550, 940
BspFNI CGCG 2 cut(s) 664, 1060
BspLI GGNNCC 1 cut(s) 549
BspT104I TTCGAA 1 cut(s) 365
BsrDI GCAATG 4 cut(s) 104, 436, 498, 1016
BsrGI TGTACA 1 cut(s) 376
BsrI ACTGG 2 cut(s) 129, 926
BssECI CCNNGG 3 cut(s) 339, 570, 622
BssMI GATC 1 cut(s) 436
BssT1I CCWWGG 1 cut(s) 570
Bst2UI CCWGG 1 cut(s) 624
Bst4CI ACNGT 2 cut(s) 809, 1055
BstAPI GCANNNNNTGC 1 cut(s) 1022
BstAUI TGTACA 1 cut(s) 376
BstBI TTCGAA 1 cut(s) 365
BstC8I GCNNGC 1 cut(s) 1023
BstDSI CCRYGG 2 cut(s) 339, 570
BstF5I GGATG 2 cut(s) 580, 967
BstFNI CGCG 2 cut(s) 664, 1060
BstHHI GCGC 1 cut(s) 1060
BstKTI GATC 1 cut(s) 439
BstMAI GTCTC 2 cut(s) 57, 741
BstMBI GATC 1 cut(s) 436
BstMWI GCNNNNNNNGC 8 cut(s) 164, 269, 661, 767, 911, 920, 1022, 1057
BstNI CCWGG 1 cut(s) 624
BstNSI RCATGY 1 cut(s) 1025
BstSCI CCNGG 1 cut(s) 622
BstSLI GKGCMC 1 cut(s) 85
BstUI CGCG 2 cut(s) 664, 1060
BstV1I GCAGC 1 cut(s) 281
BstV2I GAAGAC 1 cut(s) 269
BtgI CCRYGG 2 cut(s) 339, 570
BtsCI GGATG 2 cut(s) 580, 967
BtsI GCAGTG 2 cut(s) 85, 618
BtsIMutI CAGTG 4 cut(s) 85, 618, 814, 1051
Cac8I GCNNGC 1 cut(s) 1023
CaiI CAGNNNCTG 1 cut(s) 906
CfoI GCGC 1 cut(s) 1060
Csp6I GTAC 3 cut(s) 377, 691, 784
CviAII CATG 6 cut(s) 75, 544, 571, 831, 882, 1022
CviQI GTAC 3 cut(s) 377, 691, 784
DpnI GATC 1 cut(s) 438
DpnII GATC 1 cut(s) 436
Ecl136II GAGCTC 1 cut(s) 914
Eco130I CCWWGG 1 cut(s) 570
Eco24I GRGCYC 1 cut(s) 916
Eco53kI GAGCTC 1 cut(s) 914
EcoICRI GAGCTC 1 cut(s) 914
EcoRII CCWGG 1 cut(s) 622
EcoT14I CCWWGG 1 cut(s) 570
EcoT38I GRGCYC 1 cut(s) 916
ErhI CCWWGG 1 cut(s) 570
FaeI CATG 6 cut(s) 78, 547, 574, 834, 885, 1025
FaqI GGGAC 1 cut(s) 605
FatI CATG 6 cut(s) 74, 543, 570, 830, 881, 1021
FauNDI CATATG 1 cut(s) 972
Fnu4HI GCNGC 1 cut(s) 270
FokI GGATG 2 cut(s) 587, 974
FriOI GRGCYC 1 cut(s) 916
Fsp4HI GCNGC 1 cut(s) 270
FspBI CTAG 4 cut(s) 273, 398, 606, 857
GlaI GCGC 1 cut(s) 1059
GluI GCNGC 1 cut(s) 270
GsaI CCCAGC 1 cut(s) 348
HapII CCGG 3 cut(s) 147, 551, 941
HhaI GCGC 1 cut(s) 1060
Hin1II CATG 6 cut(s) 78, 547, 574, 834, 885, 1025
Hin6I GCGC 1 cut(s) 1058
HinP1I GCGC 1 cut(s) 1058
HindIII AAGCTT 2 cut(s) 449, 1101
HinfI GANTC 1 cut(s) 131
HpaII CCGG 3 cut(s) 147, 551, 941
HphI GGTGA 3 cut(s) 140, 737, 905
Hpy166II GTNNAC 5 cut(s) 83, 377, 392, 411, 668
Hpy188I TCNGA 2 cut(s) 142, 753
Hpy188III TCNNGA 3 cut(s) 287, 551, 941
Hpy8I GTNNAC 5 cut(s) 83, 377, 392, 411, 668
Hpy99I CGWCG 1 cut(s) 1016
HpyAV CCTTC 7 cut(s) 103, 180, 372, 758, 808, 1078, 1082
HpyCH4III ACNGT 2 cut(s) 809, 1055
HpyCH4IV ACGT 2 cut(s) 258, 1011
HpyCH4V TGCA 8 cut(s) 78, 83, 97, 102, 434, 848, 923, 1021
HpyF10VI GCNNNNNNNGC 8 cut(s) 164, 269, 661, 767, 911, 920, 1022, 1057
HpySE526I ACGT 2 cut(s) 258, 1011
Hsp92II CATG 6 cut(s) 78, 547, 574, 834, 885, 1025
HspAI GCGC 1 cut(s) 1058
Kpn2I TCCGGA 2 cut(s) 550, 940
Kzo9I GATC 1 cut(s) 436
LmnI GCTCC 6 cut(s) 50, 660, 911, 919, 998, 1041
Lsp1109I GCAGC 1 cut(s) 281
LweI GCATC 5 cut(s) 218, 565, 695, 811, 856
MaeI CTAG 4 cut(s) 273, 398, 606, 857
MaeII ACGT 2 cut(s) 258, 1011
MaeIII GTNAC 2 cut(s) 128, 834
MalI GATC 1 cut(s) 438
MboI GATC 1 cut(s) 436
MboII GAAGA 4 cut(s) 53, 202, 269, 313
MhlI GDGCHC 2 cut(s) 85, 916
MluCI AATT 5 cut(s) 151, 249, 331, 480, 951
MlyI GAGTC 1 cut(s) 125
MnlI CCTC 6 cut(s) 450, 520, 889, 1049, 1052, 1087
MroI TCCGGA 2 cut(s) 550, 940
MseI TTAA 2 cut(s) 444, 582
MspI CCGG 3 cut(s) 147, 551, 941
MspR9I CCNGG 1 cut(s) 624
Mva1269I GAATGC 1 cut(s) 246
MvaI CCWGG 1 cut(s) 624
MvnI CGCG 2 cut(s) 664, 1060
MwoI GCNNNNNNNGC 8 cut(s) 164, 269, 661, 767, 911, 920, 1022, 1057
NcoI CCATGG 1 cut(s) 570
NdeI CATATG 1 cut(s) 972
NdeII GATC 1 cut(s) 436
NlaIII CATG 6 cut(s) 78, 547, 574, 834, 885, 1025
NlaIV GGNNCC 1 cut(s) 549
NmuCI GTSAC 1 cut(s) 128
NspI RCATGY 1 cut(s) 1025
NspV TTCGAA 1 cut(s) 365
PaeI GCATGC 1 cut(s) 1025
PctI GAATGC 1 cut(s) 246
Pfl23II CGTACG 1 cut(s) 690
PflMI CCANNNNNTGG 1 cut(s) 932
PkrI GCNGC 1 cut(s) 271
PleI GAGTC 1 cut(s) 125
PpsI GAGTC 1 cut(s) 125
Psp124BI GAGCTC 1 cut(s) 916
Psp6I CCWGG 1 cut(s) 622
PspFI CCCAGC 1 cut(s) 344
PspGI CCWGG 1 cut(s) 622
PspLI CGTACG 1 cut(s) 690
PspN4I GGNNCC 1 cut(s) 549
PstNI CAGNNNCTG 1 cut(s) 906
RsaI GTAC 3 cut(s) 378, 692, 785
RsaNI GTAC 3 cut(s) 377, 691, 784
SacI GAGCTC 1 cut(s) 916
SaqAI TTAA 2 cut(s) 444, 582
SatI GCNGC 1 cut(s) 270
Sau3AI GATC 1 cut(s) 436
SchI GAGTC 1 cut(s) 125
ScrFI CCNGG 1 cut(s) 624
SduI GDGCHC 2 cut(s) 85, 916
SfaNI GCATC 5 cut(s) 218, 565, 695, 811, 856
SfuI TTCGAA 1 cut(s) 365
SmlI CTYRAG 3 cut(s) 264, 385, 452
SmoI CTYRAG 3 cut(s) 264, 385, 452
SpeI ACTAGT 1 cut(s) 397
SphI GCATGC 1 cut(s) 1025
Sse9I AATT 5 cut(s) 151, 249, 331, 480, 951
SsiI CCGC 1 cut(s) 1049
SspMI CTAG 4 cut(s) 273, 398, 606, 857
SstI GAGCTC 1 cut(s) 916
StyD4I CCNGG 1 cut(s) 622
StyI CCWWGG 1 cut(s) 570
TaaI ACNGT 2 cut(s) 809, 1055
TaiI ACGT 2 cut(s) 261, 1014
TaqI TCGA 3 cut(s) 134, 288, 365
TasI AATT 5 cut(s) 151, 249, 331, 480, 951
TatI WGTACW 1 cut(s) 376
Tru1I TTAA 2 cut(s) 444, 582
Tru9I TTAA 2 cut(s) 444, 582
TscAI CASTG 4 cut(s) 85, 618, 814, 1058
TseFI GTSAC 1 cut(s) 128
TseI GCWGC 1 cut(s) 269
Tsp45I GTSAC 1 cut(s) 128
TspDTI ATGAA 7 cut(s) 53, 63, 314, 457, 790, 959, 1092
TspGWI ACGGA 1 cut(s) 887
TspRI CASTG 4 cut(s) 85, 618, 814, 1058
Van91I CCANNNNNTGG 1 cut(s) 932
VneI GTGCAC 1 cut(s) 81
XapI RAATTY 2 cut(s) 151, 331
XceI RCATGY 1 cut(s) 1025
XspI CTAG 4 cut(s) 273, 398, 606, 857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.