Rorug01G0304500

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0006907
Physical Location & Seq
Reverse (-)
41977826 .. 41981804
3979 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0304500.1

Sequence Viewer

Length: 1068 bp
ATGGCTTCGGAGGATGTGAAACGGAGCGAAACGGCGGTCTCGACTATCGTGAATCTGGCCGAGGAGGCTAAGCTGGCGAGGGAGGGCGTCGTCAAGGCTCCCAGCCTCGCCGTTCTCAGCGTCTGCAAGTCTCTCGTCGCCGGCGGAGTCGCCGGGGGAGTGTCTCGAACAGCTGTTGCGCCATTGGAACGAATGAAAATATTGCTCCAGGTCCAAAATCCCCATAACATAAAATACAGTGGAACAGTACAAGGCCTGAAATATATCTGGAGAAGTGAGGGTTTCCGTGGACTGTTTAAAGGAAATGGTACCAATTGTGCTCGGATTGTCCCCAACTCGGCTGTCAAATTCTTCAGCTACGAGCAAGCTTCTAAGGGAATATTGTGGATGTATCGGGACAAAACTGGCAACGAAGATGCTCAGCTCACTCCTCTTCTACGACTTGGAGCTGGAGCATGTGCTGGAATTATTGCCATGTCCGCAACTTACCCAATGGACATGGTACGGGGAAGGATTACTGTACAGACAGAGTCGTCTCCCTATCAGTATAGGGGAATGTTCCATGCTCTATCAACTGTGCTTCGGGAGGAGGGTCCACGTGCTTTGTACAAGGGCTGGCTTCCTTCCGTAATTGGAGTTGTTCCGTATGTTGGACTCAACTTTGCTGTGTATGAATCCCTAAAGGATTGGTTAATTAAAAGTAGACCATTTGGACTTGTCCAAGATACAGATTTGAGCGTGACAACACGATTGGCTTGTGGGGCCGCTGCTGGAACTGTCGGGCAGACTGTTGCTTACCCACTTGATGTCATCCGTCGAAGAATGCAGATGGTGGGTTGGAGTCATGCTGCTTCCGTTGTCGCTGGTGAGGGGAGAAGCAAGGCACCACTTGAATATACTGGCATGGTTGATGCATTTAGGAAAACTGTTCGACACGAGGGCTTTGGTGCATTGTACAAGGGTTTGGTTCCCAATTCTGTTAAGGTTGTCCCATCCATTGCAATTGCATTTGTGACGTACGAGATGGTGAAGGACATTTTAGGAGTTGAGATCAGGATATCTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00901 ko01110 map00901 map01110
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

355

Amino Acids

38.46

Weight (kDa)

9.56

Isoelectric Point (pI)

35.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mito_carr PF00153 39 - 126 1.6e-24 Mitochondrial carrier protein
Mito_carr PF00153 142 - 234 4.1e-28 Mitochondrial carrier protein
Mito_carr PF00153 245 - 347 2.5e-25 Mitochondrial carrier protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 532
Acc65I GGTACC 1 cut(s) 308
AccB1I GGYRCC 2 cut(s) 308, 883
AccI GTMKAC 1 cut(s) 703
AciI CCGC 4 cut(s) 35, 144, 480, 765
AcoI YGGCCR 1 cut(s) 57
AcsI RAATTY 1 cut(s) 347
AcuI CTGAAG 1 cut(s) 337
AcvI CACGTG 1 cut(s) 599
AcyI GRCGYC 1 cut(s) 87
AfaI GTAC 7 cut(s) 249, 310, 504, 522, 608, 956, 1019
AfiI CCNNNNNNNGG 2 cut(s) 337, 650
AgsI TTSAA 1 cut(s) 893
AjnI CCWGG 1 cut(s) 207
AluBI AGCT 6 cut(s) 73, 173, 357, 368, 424, 449
AluI AGCT 6 cut(s) 73, 173, 357, 368, 424, 449
Alw21I GWGCWC 1 cut(s) 322
Alw26I GTCTC 4 cut(s) 43, 135, 168, 540
AlwNI CAGNNNCTG 1 cut(s) 123
AoxI GGCC 3 cut(s) 57, 253, 762
ApeKI GCWGC 2 cut(s) 767, 848
ApoI RAATTY 1 cut(s) 347
ArsI GACNNNNNNTTYG 2 cut(s) 21, 53
Asp718I GGTACC 1 cut(s) 308
AspLEI GCGC 1 cut(s) 181
AspS9I GGNCC 3 cut(s) 211, 593, 762
AsuC2I CCSGG 1 cut(s) 154
AsuHPI GGTGA 2 cut(s) 878, 1039
AvaII GGWCC 2 cut(s) 211, 593
BanI GGYRCC 2 cut(s) 308, 883
BauI CACGAG 1 cut(s) 935
BbrPI CACGTG 1 cut(s) 599
Bbv12I GWGCWC 1 cut(s) 322
BbvI GCAGC 2 cut(s) 754, 835
BccI CCATC 3 cut(s) 823, 1000, 1018
BceAI ACGGC 2 cut(s) 48, 95
BcgI CGANNNNNNTGC 2 cut(s) 115, 149
BciT130I CCWGG 1 cut(s) 209
BcnI CCSGG 1 cut(s) 154
BcoDI GTCTC 4 cut(s) 43, 135, 168, 540
BglI GCCNNNNNGGC 1 cut(s) 65
BisI GCNGC 3 cut(s) 765, 768, 849
BlpI GCTNAGC 2 cut(s) 69, 420
BlsI GCNGC 3 cut(s) 766, 769, 850
Bme1390I CCNGG 2 cut(s) 154, 209
Bme18I GGWCC 2 cut(s) 211, 593
BmgT120I GGNCC 3 cut(s) 211, 593, 762
BmiI GGNNCC 6 cut(s) 99, 310, 594, 763, 885, 969
BmrFI CCNGG 2 cut(s) 154, 209
BmsI GCATC 2 cut(s) 406, 901
BpmI CTGGAG 3 cut(s) 191, 289, 471
Bpu1102I GCTNAGC 2 cut(s) 69, 420
BpuMI CCSGG 1 cut(s) 154
BsaAI YACGTR 1 cut(s) 599
BsaHI GRCGYC 1 cut(s) 87
BsaI GGTCTC 1 cut(s) 43
BsaJI CCNNGG 3 cut(s) 60, 153, 286
Bsc4I CCNNNNNNNGG 2 cut(s) 337, 650
Bse118I RCCGGY 1 cut(s) 140
Bse1I ACTGG 2 cut(s) 409, 904
Bse3DI GCAATG 1 cut(s) 996
BseBI CCWGG 1 cut(s) 209
BseDI CCNNGG 3 cut(s) 60, 153, 286
BseGI GGATG 4 cut(s) 19, 393, 810, 992
BseLI CCNNNNNNNGG 2 cut(s) 337, 650
BseMI GCAATG 1 cut(s) 996
BseMII CTCAG 2 cut(s) 130, 434
BseNI ACTGG 2 cut(s) 409, 904
BseRI GAGGAG 3 cut(s) 77, 420, 602
BseXI GCAGC 2 cut(s) 754, 835
BseYI CCCAGC 1 cut(s) 101
BshFI GGCC 3 cut(s) 59, 255, 764
BshNI GGYRCC 2 cut(s) 308, 883
BsiHKAI GWGCWC 1 cut(s) 322
BsiSI CCGG 2 cut(s) 141, 153
BsiWI CGTACG 1 cut(s) 1017
BslFI GGGAC 3 cut(s) 314, 410, 974
BslI CCNNNNNNNGG 2 cut(s) 337, 650
BsmAI GTCTC 4 cut(s) 43, 135, 168, 540
BsmBI CGTCTC 1 cut(s) 540
BsmFI GGGAC 3 cut(s) 314, 410, 974
BsmI GAATGC 1 cut(s) 828
BsnI GGCC 3 cut(s) 59, 255, 764
Bso31I GGTCTC 1 cut(s) 43
Bsp1286I GDGCHC 1 cut(s) 322
Bsp1407I TGTACA 3 cut(s) 520, 606, 954
Bsp143I GATC 1 cut(s) 1050
Bsp1720I GCTNAGC 2 cut(s) 69, 420
BspACI CCGC 4 cut(s) 35, 144, 480, 765
BspANI GGCC 3 cut(s) 59, 255, 764
BspCNI CTCAG 2 cut(s) 129, 433
BspLI GGNNCC 6 cut(s) 99, 310, 594, 763, 885, 969
BspT107I GGYRCC 2 cut(s) 308, 883
BspTNI GGTCTC 1 cut(s) 43
BsrDI GCAATG 1 cut(s) 996
BsrFI RCCGGY 1 cut(s) 140
BsrGI TGTACA 3 cut(s) 520, 606, 954
BsrI ACTGG 2 cut(s) 409, 904
BssAI RCCGGY 1 cut(s) 140
BssECI CCNNGG 3 cut(s) 60, 153, 286
BssMI GATC 1 cut(s) 1050
BssNI GRCGYC 1 cut(s) 87
BssSI CACGAG 1 cut(s) 935
Bst2BI CACGAG 1 cut(s) 935
Bst2UI CCWGG 1 cut(s) 209
Bst4CI ACNGT 8 cut(s) 239, 247, 294, 520, 577, 778, 790, 928
Bst6I CTCTTC 1 cut(s) 438
BstACI GRCGYC 1 cut(s) 87
BstAUI TGTACA 3 cut(s) 520, 606, 954
BstBAI YACGTR 1 cut(s) 599
BstC8I GCNNGC 4 cut(s) 75, 142, 366, 617
BstDEI CTNAG 4 cut(s) 69, 116, 372, 420
BstDSI CCRYGG 1 cut(s) 286
BstF5I GGATG 4 cut(s) 19, 393, 810, 992
BstHHI GCGC 1 cut(s) 181
BstKTI GATC 1 cut(s) 1053
BstMAI GTCTC 4 cut(s) 43, 135, 168, 540
BstMBI GATC 1 cut(s) 1050
BstMWI GCNNNNNNNGC 4 cut(s) 65, 74, 479, 761
BstNI CCWGG 1 cut(s) 209
BstNSI RCATGY 1 cut(s) 459
BstSCI CCNGG 2 cut(s) 152, 207
BstV1I GCAGC 2 cut(s) 754, 835
BsuRI GGCC 3 cut(s) 59, 255, 764
BtgI CCRYGG 1 cut(s) 286
BtsCI GGATG 4 cut(s) 19, 393, 810, 992
BtsIMutI CAGTG 1 cut(s) 244
Cac8I GCNNGC 4 cut(s) 75, 142, 366, 617
CaiI CAGNNNCTG 1 cut(s) 123
CfoI GCGC 1 cut(s) 181
Cfr10I RCCGGY 1 cut(s) 140
Cfr13I GGNCC 3 cut(s) 211, 593, 762
CseI GACGC 2 cut(s) 76, 109
Csp6I GTAC 7 cut(s) 248, 309, 503, 521, 607, 955, 1018
CspCI CAANNNNNGTGG 2 cut(s) 585, 620
CviAII CATG 6 cut(s) 456, 475, 499, 563, 845, 904
CviQI GTAC 7 cut(s) 248, 309, 503, 521, 607, 955, 1018
DdeI CTNAG 4 cut(s) 69, 116, 372, 420
DpnI GATC 1 cut(s) 1052
DpnII GATC 1 cut(s) 1050
DraI TTTAAA 1 cut(s) 298
DrdI GACNNNNNNGTC 1 cut(s) 532
DseDI GACNNNNNNGTC 1 cut(s) 532
EaeI YGGCCR 1 cut(s) 57
Eam1104I CTCTTC 1 cut(s) 438
EarI CTCTTC 1 cut(s) 438
EciI GGCGGA 1 cut(s) 159
Eco147I AGGCCT 1 cut(s) 255
Eco31I GGTCTC 1 cut(s) 43
Eco32I GATATC 1 cut(s) 1059
Eco47I GGWCC 2 cut(s) 211, 593
Eco57I CTGAAG 1 cut(s) 337
Eco72I CACGTG 1 cut(s) 599
EcoRII CCWGG 1 cut(s) 207
EcoRV GATATC 1 cut(s) 1059
EcoT22I ATGCAT 1 cut(s) 916
Esp3I CGTCTC 1 cut(s) 540
FaeI CATG 6 cut(s) 459, 478, 502, 566, 848, 907
FaqI GGGAC 3 cut(s) 314, 410, 974
FatI CATG 6 cut(s) 455, 474, 498, 562, 844, 903
FblI GTMKAC 1 cut(s) 703
Fnu4HI GCNGC 3 cut(s) 765, 768, 849
FokI GGATG 4 cut(s) 26, 400, 797, 979
Fsp4HI GCNGC 3 cut(s) 765, 768, 849
GlaI GCGC 1 cut(s) 180
GluI GCNGC 3 cut(s) 765, 768, 849
GsaI CCCAGC 1 cut(s) 105
GsuI CTGGAG 3 cut(s) 191, 289, 471
HaeIII GGCC 3 cut(s) 59, 255, 764
HapII CCGG 2 cut(s) 141, 153
HgaI GACGC 2 cut(s) 76, 109
HhaI GCGC 1 cut(s) 181
Hin1I GRCGYC 1 cut(s) 87
Hin1II CATG 6 cut(s) 459, 478, 502, 566, 848, 907
Hin6I GCGC 1 cut(s) 179
HinP1I GCGC 1 cut(s) 179
HindIII AAGCTT 1 cut(s) 366
HinfI GANTC 6 cut(s) 52, 147, 530, 654, 674, 841
HpaII CCGG 2 cut(s) 141, 153
HphI GGTGA 2 cut(s) 878, 1039
Hpy166II GTNNAC 3 cut(s) 290, 596, 704
Hpy188I TCNGA 3 cut(s) 10, 324, 1063
Hpy188III TCNNGA 7 cut(s) 40, 49, 165, 268, 395, 584, 1054
Hpy8I GTNNAC 3 cut(s) 290, 596, 704
Hpy99I CGWCG 3 cut(s) 92, 140, 819
HpyAV CCTTC 3 cut(s) 504, 633, 1024
HpyCH4III ACNGT 8 cut(s) 239, 247, 294, 520, 577, 778, 790, 928
HpyCH4IV ACGT 2 cut(s) 598, 1016
HpyCH4V TGCA 6 cut(s) 126, 826, 914, 950, 1001, 1007
HpyF10VI GCNNNNNNNGC 4 cut(s) 65, 74, 479, 761
HpyF3I CTNAG 4 cut(s) 69, 116, 372, 420
HpySE526I ACGT 2 cut(s) 598, 1016
Hsp92I GRCGYC 1 cut(s) 87
Hsp92II CATG 6 cut(s) 459, 478, 502, 566, 848, 907
HspAI GCGC 1 cut(s) 179
KpnI GGTACC 1 cut(s) 312
KroI GCCGGC 1 cut(s) 140
KroNI GCCGGC 1 cut(s) 142
Kzo9I GATC 1 cut(s) 1050
LmnI GCTCC 5 cut(s) 24, 103, 210, 446, 452
Lsp1109I GCAGC 2 cut(s) 754, 835
LweI GCATC 2 cut(s) 406, 901
MaeII ACGT 2 cut(s) 598, 1016
MaeIII GTNAC 2 cut(s) 739, 1012
MalI GATC 1 cut(s) 1052
MboI GATC 1 cut(s) 1050
MboII GAAGA 4 cut(s) 343, 425, 425, 831
MfeI CAATTG 2 cut(s) 313, 1002
MhlI GDGCHC 1 cut(s) 322
MluCI AATT 7 cut(s) 313, 347, 465, 630, 693, 973, 1002
MlyI GAGTC 4 cut(s) 156, 539, 648, 850
MmeI TCCRAC 2 cut(s) 631, 818
Mph1103I ATGCAT 1 cut(s) 916
MreI CGCCGGCG 1 cut(s) 140
MroNI GCCGGC 1 cut(s) 140
MseI TTAA 4 cut(s) 297, 692, 696, 981
MspA1I CMGCKG 2 cut(s) 173, 767
MspI CCGG 2 cut(s) 141, 153
MspR9I CCNGG 2 cut(s) 154, 209
MunI CAATTG 2 cut(s) 313, 1002
Mva1269I GAATGC 1 cut(s) 828
MvaI CCWGG 1 cut(s) 209
MwoI GCNNNNNNNGC 4 cut(s) 65, 74, 479, 761
NaeI GCCGGC 1 cut(s) 142
NciI CCSGG 1 cut(s) 154
NdeII GATC 1 cut(s) 1050
NgoMIV GCCGGC 1 cut(s) 140
NlaIII CATG 6 cut(s) 459, 478, 502, 566, 848, 907
NlaIV GGNNCC 6 cut(s) 99, 310, 594, 763, 885, 969
NmeAIII GCCGAG 2 cut(s) 85, 317
NmuCI GTSAC 2 cut(s) 739, 1012
NsiI ATGCAT 1 cut(s) 916
NspI RCATGY 1 cut(s) 459
PacI TTAATTAA 1 cut(s) 696
PceI AGGCCT 1 cut(s) 255
PcsI WCGNNNNNNNCGW 1 cut(s) 38
PctI GAATGC 1 cut(s) 828
PdiI GCCGGC 1 cut(s) 142
PfeI GAWTC 2 cut(s) 52, 674
Pfl23II CGTACG 1 cut(s) 1017
PflFI GACNNNGTC 1 cut(s) 529
PkrI GCNGC 3 cut(s) 766, 769, 850
PleI GAGTC 4 cut(s) 155, 538, 648, 849
PmaCI CACGTG 1 cut(s) 599
PmlI CACGTG 1 cut(s) 599
PpsI GAGTC 4 cut(s) 155, 538, 648, 849
Ppu21I YACGTR 1 cut(s) 599
Psp6I CCWGG 1 cut(s) 207
PspCI CACGTG 1 cut(s) 599
PspFI CCCAGC 1 cut(s) 101
PspGI CCWGG 1 cut(s) 207
PspLI CGTACG 1 cut(s) 1017
PspN4I GGNNCC 6 cut(s) 99, 310, 594, 763, 885, 969
PspPI GGNCC 3 cut(s) 211, 593, 762
PstNI CAGNNNCTG 1 cut(s) 123
PsyI GACNNNGTC 1 cut(s) 529
PvuII CAGCTG 1 cut(s) 173
RsaI GTAC 7 cut(s) 249, 310, 504, 522, 608, 956, 1019
RsaNI GTAC 7 cut(s) 248, 309, 503, 521, 607, 955, 1018
SaqAI TTAA 4 cut(s) 297, 692, 696, 981
SatI GCNGC 3 cut(s) 765, 768, 849
Sau3AI GATC 1 cut(s) 1050
Sau96I GGNCC 3 cut(s) 211, 593, 762
SchI GAGTC 4 cut(s) 156, 539, 648, 850
ScrFI CCNGG 2 cut(s) 154, 209
SduI GDGCHC 1 cut(s) 322
SfaNI GCATC 2 cut(s) 406, 901
SgrAI CRCCGGYG 1 cut(s) 140
SinI GGWCC 2 cut(s) 211, 593
Sse9I AATT 7 cut(s) 313, 347, 465, 630, 693, 973, 1002
SseBI AGGCCT 1 cut(s) 255
SsiI CCGC 4 cut(s) 35, 144, 480, 765
SspI AATATT 2 cut(s) 201, 381
StuI AGGCCT 1 cut(s) 255
StyD4I CCNGG 2 cut(s) 152, 207
TaaI ACNGT 8 cut(s) 239, 247, 294, 520, 577, 778, 790, 928
TaiI ACGT 2 cut(s) 601, 1019
TaqI TCGA 4 cut(s) 41, 166, 817, 931
TasI AATT 7 cut(s) 313, 347, 465, 630, 693, 973, 1002
TatI WGTACW 4 cut(s) 247, 520, 606, 954
TauI GCSGC 1 cut(s) 767
TfiI GAWTC 2 cut(s) 52, 674
Tru1I TTAA 4 cut(s) 297, 692, 696, 981
Tru9I TTAA 4 cut(s) 297, 692, 696, 981
TscAI CASTG 1 cut(s) 244
TseFI GTSAC 2 cut(s) 739, 1012
TseI GCWGC 2 cut(s) 767, 848
Tsp45I GTSAC 2 cut(s) 739, 1012
TspDTI ATGAA 2 cut(s) 209, 687
TspGWI ACGGA 6 cut(s) 37, 275, 616, 633, 803, 844
TspRI CASTG 1 cut(s) 244
Tth111I GACNNNGTC 1 cut(s) 529
VpaK11BI GGWCC 2 cut(s) 211, 593
XapI RAATTY 1 cut(s) 347
XceI RCATGY 1 cut(s) 459
XmiI GTMKAC 1 cut(s) 703
Zsp2I ATGCAT 1 cut(s) 916
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.