RchiOBHm_Chr1g0363081

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0017137
Physical Location & Seq
Forward (+)
54589271 .. 54590861
1591 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ58786

Sequence Viewer

Length: 1128 bp
ATGATTTGTGGCATAACTCAAAACAAAGCATTGTTTCTCCTCTCCTCGATGGCAACCAAAGCAAGCCTACTTTTCATGCTTAGCTCCTTGTTTCTTCTTTGGAAGTTAAAAGTTAACTCCTTGTTTCTTCTTCGGAATTTAAGCAATGCAGCATCATTGAAAGCTTGTAACTTGGAAGCCATATATCAATTTGGGGACTCGTTCTCCGACACCGGAAATGCGATACGTGAAAACAAAGCTCTAGAGGCTACTAAGCTCCCTTATGGCTTAAATCTTCCCAGGGAACCAAAAGGTCGATTTTCCGACGGGATGTTGGTTATAGATTTTGAAGCGAAAGATTACCACAACGGAAAGAACTTTGCTGTTAGTTCTGCTAGCGTTTTGCCAAAAGGTGTTCTTAAAGAGAAATCAATATTCTTCCCAGAAACAATGACAAACTTTACGCTACATAAGCAAGTGGATTGGATGGCCACTTACTTCAATGAAACCCCCTATTTTAGTAAAGACCGCGCAGAGAAGCTAAAGAGGTCACTCTTTTTCATCGGACCAATGGCACATGGTGATTATATGTATTCAATGGACTTGAGTCGTAAATCTTCAGAATGGGTAAAGAGCAATTTGGTTCCAGATATCGTCCAAACCATCAAAGAAGCTGCCACAAGGGTCATTGCTTTTGGTGCTGTGAAAGTGGTCATCCCAGGAATATACGCATTTGGTTGTTTTAAAGCTCCAGGTTATAATGTATCGCTATGCGACCAGGGCAAAAATAGTGTTGCAACCACTCACAACGATCTGCTTCGGAGAGCAATTGAGGAGTTAAAGAAACAACACCCTAATGTGACTATTGTATATGCAGATTATTACAGTGCATTCATGTCCTTCTTCCCCAAGGCACTTAGTCTAGGGTTTGAACCTGCGAACAAGCCATGCTGTTTCGGTACACATAAAAATGGTCCAGGACATGGTTTTAACCCGGATAACATGTGTGGAAAGCCTGGAGTACAATCTTGTGCTAACCCTGCTAAACGAATCAACTGGGATGGTAGTCATATGACGCAACATGCATACAGGTTAATAGCAGATTTGATAATTCCTGACATCGCAGCCAAGCTTAAATGCAGTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00901 ko01110 map00901 map01110
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

375

Amino Acids

41.69

Weight (kDa)

9.22

Isoelectric Point (pI)

28.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 61 - 363 2e-19 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017137)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G03980
fragaria_vesca FvH4_4g05740
prunus_persica Prupe.4G256100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0363081
rosa_laevigata RLG00000027595
rosa_multiflora Rmu_sc0004472.1_g000006
rosa_roxburghii Rroxscaffold_4G00293480
rosa_rugosa Rorug01G0304500
rosa_samantha Rh1AG313500 Rh1BG276700 Rh1CG293400 Rh1DG307400
rosa_wichuraiana Rw1G027770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 738
Acc36I ACCTGC 1 cut(s) 922
AccB7I CCANNNNNTGG 1 cut(s) 962
AccII CGCG 1 cut(s) 510
AciI CCGC 1 cut(s) 508
AcoI YGGCCR 1 cut(s) 468
AcsI RAATTY 1 cut(s) 136
AcuI CTGAAG 1 cut(s) 582
AdeI CACNNNGTG 1 cut(s) 560
AfaI GTAC 2 cut(s) 940, 1002
AfiI CCNNNNNNNGG 1 cut(s) 962
AflIII ACRYGT 1 cut(s) 981
AgsI TTSAA 5 cut(s) 160, 329, 481, 576, 911
AjnI CCWGG 6 cut(s) 278, 697, 730, 756, 955, 994
AluBI AGCT 8 cut(s) 84, 164, 239, 256, 520, 653, 728, 1111
AluI AGCT 8 cut(s) 84, 164, 239, 256, 520, 653, 728, 1111
AoxI GGCC 1 cut(s) 468
ApeKI GCWGC 3 cut(s) 149, 653, 1103
ApoI RAATTY 1 cut(s) 136
AspLEI GCGC 1 cut(s) 512
AspS9I GGNCC 2 cut(s) 545, 953
AsuC2I CCSGG 1 cut(s) 974
AsuHPI GGTGA 1 cut(s) 572
AsuNHI GCTAGC 1 cut(s) 374
AvaII GGWCC 2 cut(s) 545, 953
BalI TGGCCA 1 cut(s) 470
BbvI GCAGC 3 cut(s) 161, 640, 1115
BccI CCATC 4 cut(s) 43, 460, 650, 1034
BciT130I CCWGG 6 cut(s) 280, 699, 732, 758, 957, 996
BcnI CCSGG 1 cut(s) 974
BfaI CTAG 3 cut(s) 242, 375, 902
BfuAI ACCTGC 1 cut(s) 922
BisI GCNGC 3 cut(s) 150, 654, 1104
BlpI GCTNAGC 1 cut(s) 80
BlsI GCNGC 3 cut(s) 151, 655, 1105
Bme1390I CCNGG 7 cut(s) 280, 699, 732, 758, 957, 974, 996
Bme18I GGWCC 2 cut(s) 545, 953
BmgT120I GGNCC 2 cut(s) 545, 953
BmiI GGNNCC 2 cut(s) 285, 624
BmrFI CCNGG 7 cut(s) 280, 699, 732, 758, 957, 974, 996
BmrI ACTGGG 1 cut(s) 1045
BmsI GCATC 1 cut(s) 161
BmtI GCTAGC 1 cut(s) 378
BmuI ACTGGG 1 cut(s) 1045
BoxI GACNNNNGTC 1 cut(s) 585
BpmI CTGGAG 2 cut(s) 714, 1017
Bpu1102I GCTNAGC 1 cut(s) 80
BpuEI CTTGAG 1 cut(s) 604
BpuMI CCSGG 1 cut(s) 974
BsaAI YACGTR 1 cut(s) 227
BsaJI CCNNGG 5 cut(s) 278, 279, 697, 757, 888
BsaWI WCCGGW 1 cut(s) 212
BsaXI ACNNNNNCTCC 2 cut(s) 188, 218
Bsc4I CCNNNNNNNGG 1 cut(s) 962
Bse1I ACTGG 1 cut(s) 1040
Bse3DI GCAATG 2 cut(s) 151, 666
BseBI CCWGG 6 cut(s) 280, 699, 732, 758, 957, 996
BseDI CCNNGG 5 cut(s) 278, 279, 697, 757, 888
BseGI GGATG 4 cut(s) 315, 471, 693, 1045
BseLI CCNNNNNNNGG 1 cut(s) 962
BseMI GCAATG 2 cut(s) 151, 666
BseNI ACTGG 1 cut(s) 1040
BseRI GAGGAG 3 cut(s) 29, 34, 827
BseXI GCAGC 3 cut(s) 161, 640, 1115
Bsh1236I CGCG 1 cut(s) 510
BshFI GGCC 1 cut(s) 470
BsiSI CCGG 2 cut(s) 213, 974
BslFI GGGAC 1 cut(s) 209
BslI CCNNNNNNNGG 1 cut(s) 962
BsmFI GGGAC 1 cut(s) 209
BsmI GAATGC 1 cut(s) 869
BsnI GGCC 1 cut(s) 470
Bsp143I GATC 1 cut(s) 790
Bsp1720I GCTNAGC 1 cut(s) 80
BspACI CCGC 1 cut(s) 508
BspANI GGCC 1 cut(s) 470
BspFNI CGCG 1 cut(s) 510
BspLI GGNNCC 2 cut(s) 285, 624
BspMI ACCTGC 1 cut(s) 922
BspOI GCTAGC 1 cut(s) 378
BsrDI GCAATG 2 cut(s) 151, 666
BsrI ACTGG 1 cut(s) 1040
BssECI CCNNGG 5 cut(s) 278, 279, 697, 757, 888
BssMI GATC 1 cut(s) 790
BssT1I CCWWGG 1 cut(s) 888
Bst2UI CCWGG 6 cut(s) 280, 699, 732, 758, 957, 996
Bst4CI ACNGT 1 cut(s) 866
BstBAI YACGTR 1 cut(s) 227
BstC8I GCNNGC 2 cut(s) 64, 376
BstDEI CTNAG 3 cut(s) 80, 252, 896
BstF5I GGATG 4 cut(s) 315, 471, 693, 1045
BstFNI CGCG 1 cut(s) 510
BstHHI GCGC 1 cut(s) 512
BstKTI GATC 1 cut(s) 793
BstMBI GATC 1 cut(s) 790
BstMWI GCNNNNNNNGC 6 cut(s) 59, 245, 451, 677, 759, 1019
BstNI CCWGG 6 cut(s) 280, 699, 732, 758, 957, 996
BstNSI RCATGY 2 cut(s) 985, 1064
BstPAI GACNNNNGTC 1 cut(s) 585
BstSCI CCNGG 7 cut(s) 278, 697, 730, 756, 955, 972, 994
BstUI CGCG 1 cut(s) 510
BstV1I GCAGC 3 cut(s) 161, 640, 1115
BsuRI GGCC 1 cut(s) 470
BtgZI GCGATG 1 cut(s) 1084
BtsCI GGATG 4 cut(s) 315, 471, 693, 1045
BtsI GCAGTG 1 cut(s) 1126
BtsIMutI CAGTG 2 cut(s) 871, 1126
BveI ACCTGC 1 cut(s) 922
Cac8I GCNNGC 2 cut(s) 64, 376
CfoI GCGC 1 cut(s) 512
Cfr13I GGNCC 2 cut(s) 545, 953
CseI GACGC 1 cut(s) 1063
Csp6I GTAC 2 cut(s) 939, 1001
CviAII CATG 7 cut(s) 76, 557, 874, 927, 962, 982, 1061
CviQI GTAC 2 cut(s) 939, 1001
DdeI CTNAG 3 cut(s) 80, 252, 896
DpnI GATC 1 cut(s) 792
DpnII GATC 1 cut(s) 790
DraI TTTAAA 1 cut(s) 724
DraIII CACNNNGTG 1 cut(s) 560
EaeI YGGCCR 1 cut(s) 468
Eco130I CCWWGG 1 cut(s) 888
Eco32I GATATC 1 cut(s) 631
Eco47I GGWCC 2 cut(s) 545, 953
Eco57I CTGAAG 1 cut(s) 582
EcoRII CCWGG 6 cut(s) 278, 697, 730, 756, 955, 994
EcoRV GATATC 1 cut(s) 631
EcoT14I CCWWGG 1 cut(s) 888
EcoT22I ATGCAT 1 cut(s) 1066
ErhI CCWWGG 1 cut(s) 888
FaeI CATG 7 cut(s) 79, 560, 877, 930, 965, 985, 1064
FalI AAGNNNNNCTT 2 cut(s) 381, 413
FaqI GGGAC 1 cut(s) 209
FatI CATG 7 cut(s) 75, 556, 873, 926, 961, 981, 1060
FauNDI CATATG 1 cut(s) 1050
Fnu4HI GCNGC 3 cut(s) 150, 654, 1104
FokI GGATG 4 cut(s) 322, 478, 680, 1052
Fsp4HI GCNGC 3 cut(s) 150, 654, 1104
FspBI CTAG 3 cut(s) 242, 375, 902
GlaI GCGC 1 cut(s) 511
GluI GCNGC 3 cut(s) 150, 654, 1104
GsuI CTGGAG 2 cut(s) 714, 1017
HaeIII GGCC 1 cut(s) 470
HapII CCGG 2 cut(s) 213, 974
HgaI GACGC 1 cut(s) 1063
HhaI GCGC 1 cut(s) 512
Hin1II CATG 7 cut(s) 79, 560, 877, 930, 965, 985, 1064
Hin6I GCGC 1 cut(s) 510
HinP1I GCGC 1 cut(s) 510
HincII GTYRAC 1 cut(s) 115
HindII GTYRAC 1 cut(s) 115
HindIII AAGCTT 2 cut(s) 162, 1109
HinfI GANTC 3 cut(s) 197, 586, 1029
HpaI GTTAAC 1 cut(s) 115
HpaII CCGG 2 cut(s) 213, 974
HphI GGTGA 1 cut(s) 572
Hpy166II GTNNAC 2 cut(s) 115, 941
Hpy188I TCNGA 6 cut(s) 135, 208, 304, 545, 601, 801
Hpy188III TCNNGA 3 cut(s) 242, 626, 1094
Hpy8I GTNNAC 2 cut(s) 115, 941
Hpy99I CGWCG 1 cut(s) 308
HpyAV CCTTC 1 cut(s) 889
HpyCH4III ACNGT 1 cut(s) 866
HpyCH4IV ACGT 1 cut(s) 226
HpyCH4V TGCA 7 cut(s) 149, 776, 854, 869, 1064, 1119, 1124
HpyF10VI GCNNNNNNNGC 6 cut(s) 59, 245, 451, 677, 759, 1019
HpyF3I CTNAG 3 cut(s) 80, 252, 896
HpySE526I ACGT 1 cut(s) 226
Hsp92II CATG 7 cut(s) 79, 560, 877, 930, 965, 985, 1064
HspAI GCGC 1 cut(s) 510
KspAI GTTAAC 1 cut(s) 115
Kzo9I GATC 1 cut(s) 790
LmnI GCTCC 3 cut(s) 89, 261, 733
Lsp1109I GCAGC 3 cut(s) 161, 640, 1115
LweI GCATC 1 cut(s) 161
MaeI CTAG 3 cut(s) 242, 375, 902
MaeII ACGT 1 cut(s) 226
MaeIII GTNAC 3 cut(s) 167, 528, 838
MalI GATC 1 cut(s) 792
MboI GATC 1 cut(s) 790
MboII GAAGA 7 cut(s) 86, 119, 122, 266, 409, 588, 874
MfeI CAATTG 1 cut(s) 807
MlsI TGGCCA 1 cut(s) 470
MluCI AATT 5 cut(s) 136, 188, 616, 807, 1089
MluNI TGGCCA 1 cut(s) 470
MlyI GAGTC 2 cut(s) 191, 595
MmeI TCCRAC 2 cut(s) 231, 327
MnlI CCTC 5 cut(s) 50, 55, 238, 519, 805
Mox20I TGGCCA 1 cut(s) 470
Mph1103I ATGCAT 1 cut(s) 1066
MscI TGGCCA 1 cut(s) 470
MslI CAYNNNNRTG 2 cut(s) 834, 948
Msp20I TGGCCA 1 cut(s) 470
MspI CCGG 2 cut(s) 213, 974
MspR9I CCNGG 7 cut(s) 280, 699, 732, 758, 957, 974, 996
MunI CAATTG 1 cut(s) 807
Mva1269I GAATGC 1 cut(s) 869
MvaI CCWGG 6 cut(s) 280, 699, 732, 758, 957, 996
MvnI CGCG 1 cut(s) 510
MwoI GCNNNNNNNGC 6 cut(s) 59, 245, 451, 677, 759, 1019
NciI CCSGG 1 cut(s) 974
NdeI CATATG 1 cut(s) 1050
NdeII GATC 1 cut(s) 790
NheI GCTAGC 1 cut(s) 374
NlaIII CATG 7 cut(s) 79, 560, 877, 930, 965, 985, 1064
NlaIV GGNNCC 2 cut(s) 285, 624
NmuCI GTSAC 2 cut(s) 528, 838
NsiI ATGCAT 1 cut(s) 1066
NspI RCATGY 2 cut(s) 985, 1064
PasI CCCWGGG 1 cut(s) 279
PciI ACATGT 1 cut(s) 981
PctI GAATGC 1 cut(s) 869
PfeI GAWTC 1 cut(s) 1029
PflMI CCANNNNNTGG 1 cut(s) 962
PfoI TCCNGGA 1 cut(s) 955
PkrI GCNGC 3 cut(s) 151, 655, 1105
PleI GAGTC 2 cut(s) 191, 594
PpsI GAGTC 2 cut(s) 191, 594
Ppu21I YACGTR 1 cut(s) 227
PscI ACATGT 1 cut(s) 981
PshAI GACNNNNGTC 1 cut(s) 585
PsiI TTATAA 1 cut(s) 738
Psp6I CCWGG 6 cut(s) 278, 697, 730, 756, 955, 994
PspGI CCWGG 6 cut(s) 278, 697, 730, 756, 955, 994
PspN4I GGNNCC 2 cut(s) 285, 624
PspPI GGNCC 2 cut(s) 545, 953
RsaI GTAC 2 cut(s) 940, 1002
RsaNI GTAC 2 cut(s) 939, 1001
RseI CAYNNNNRTG 2 cut(s) 834, 948
SatI GCNGC 3 cut(s) 150, 654, 1104
Sau3AI GATC 1 cut(s) 790
Sau96I GGNCC 2 cut(s) 545, 953
SchI GAGTC 2 cut(s) 191, 595
ScrFI CCNGG 7 cut(s) 280, 699, 732, 758, 957, 974, 996
SfaNI GCATC 1 cut(s) 161
SinI GGWCC 2 cut(s) 545, 953
SmiMI CAYNNNNRTG 2 cut(s) 834, 948
SmlI CTYRAG 1 cut(s) 583
SmoI CTYRAG 1 cut(s) 583
Sse9I AATT 5 cut(s) 136, 188, 616, 807, 1089
SsiI CCGC 1 cut(s) 508
SspI AATATT 1 cut(s) 414
SspMI CTAG 3 cut(s) 242, 375, 902
StyD4I CCNGG 7 cut(s) 278, 697, 730, 756, 955, 972, 994
StyI CCWWGG 1 cut(s) 888
TaaI ACNGT 1 cut(s) 866
TaiI ACGT 1 cut(s) 229
TaqI TCGA 2 cut(s) 47, 295
TasI AATT 5 cut(s) 136, 188, 616, 807, 1089
TatI WGTACW 1 cut(s) 1000
TfiI GAWTC 1 cut(s) 1029
TscAI CASTG 2 cut(s) 871, 1126
TseFI GTSAC 2 cut(s) 528, 838
TseI GCWGC 3 cut(s) 149, 653, 1103
Tsp45I GTSAC 2 cut(s) 528, 838
TspDTI ATGAA 4 cut(s) 64, 498, 529, 862
TspGWI ACGGA 1 cut(s) 363
TspRI CASTG 2 cut(s) 871, 1126
Van91I CCANNNNNTGG 1 cut(s) 962
VpaK11BI GGWCC 2 cut(s) 545, 953
XapI RAATTY 1 cut(s) 136
XbaI TCTAGA 1 cut(s) 241
XceI RCATGY 2 cut(s) 985, 1064
XspI CTAG 3 cut(s) 242, 375, 902
Zsp2I ATGCAT 1 cut(s) 1066
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.