Rw1G027770

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0017137
Physical Location & Seq
Forward (+)
55253529 .. 55255453
1925 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G027770.1

Sequence Viewer

Length: 1122 bp
ATGGCAACCAAAGCAAGCCTATTTTTCATGCTTAGCTCCTTGTTTCTTCTTTGGAAGGTTAACTCCTCATTTCTTCTTCGGAATTTAAGCAATGCAGCATCATTGAAAGCTTGTAACTTGGAAGCCATATATCAATTTGGGGACTCTTTCTCCGACACCGGAAATGCGATACGTGAAAACAAAGCTCTAGAGGCTACTAAGCTCCCTTATGGCTTAAATCTTCCCAGGGAACCAAAAGGTCGATTTTCCGACGGGATGTTGGTTATAGATTTTGAAGCACAAGCAGCTGAGCTTCCATTTCTTAAACCATATTTGGATCAGGAAGCGAAAGATTACCACAACGGAAAGAACTTTGCTGTTAGTTCTGCTAGCGTTTTGCCAAAAGGTGTTCTTAAAGAGAAATCAATACTCTTCCCAGAAACAATGACAAACATTACGCTACATAAGCAAGTGGATTGGATGGCCACTTACTTCAATGAAACCCCCTATTTTAGTCAAGACCGCGCAGAGAAGCTTAAGAGGTCACTCTTTTTCATCGGACCAATGGCACATGGTGATTATATGTATTCAATGGACTTGAGTCGTAAATCTTCAGAATGGGTAAAGAGCAATTTGGTTCCAGATATCGTCCAAACCATCAAAGAAGCTGCCACAAGGGTCATTGCTTTTGGTGCTGTGAAAGTGGTCATCCCAGGAATATACGCATTTGGTTGTTTTAAAGCTCCAGGTTATAATGTATCGCTATGCGACCAGGGCAAAAATAGTGTTGCAACCACTCACAACGATCTGCTTCGGAAAGCAATTGAGGAGTTAAAGAAACAACACCCTAATGTGACTATTGTATATGCAGATTATTACAGTGCATTCATGTCCTTCTTCCCCAAGGCACTTAGTCTAGGGTTTGAACCTGCGAACAAGCCATGCTGTTTCGGCACACATAAAAATGGTCCAGGACATGGTTTTAACCCGGATAACATGTGTGGAAAGCCTGGAGTACAAGCTTGTGCTAACCCTGCGAAACGAATCAACTGGGATGGTAGTCATATGACGCAACATGCATACAAGTTAATAGCAGATTTGATAATTCCTGACATCGCAGCCAAGCTTAAATGCAGTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00901 ko01110 map00901 map01110
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

373

Amino Acids

41.41

Weight (kDa)

8.91

Isoelectric Point (pI)

30.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 43 - 361 2.2e-22 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017137)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G03980
fragaria_vesca FvH4_4g05740
prunus_persica Prupe.4G256100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0363081
rosa_laevigata RLG00000027595
rosa_multiflora Rmu_sc0004472.1_g000006
rosa_roxburghii Rroxscaffold_4G00293480
rosa_rugosa Rorug01G0304500
rosa_samantha Rh1AG313500 Rh1BG276700 Rh1CG293400 Rh1DG307400
rosa_wichuraiana Rw1G027770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 732
Acc36I ACCTGC 1 cut(s) 916
AccB7I CCANNNNNTGG 1 cut(s) 956
AccII CGCG 1 cut(s) 504
AciI CCGC 1 cut(s) 502
AclWI GGATC 1 cut(s) 324
AcoI YGGCCR 1 cut(s) 462
AcsI RAATTY 1 cut(s) 82
AcuI CTGAAG 1 cut(s) 576
AdeI CACNNNGTG 1 cut(s) 554
AfaI GTAC 1 cut(s) 996
AfiI CCNNNNNNNGG 1 cut(s) 956
AflII CTTAAG 1 cut(s) 515
AflIII ACRYGT 1 cut(s) 975
AgsI TTSAA 5 cut(s) 106, 275, 475, 570, 905
AjnI CCWGG 6 cut(s) 224, 691, 724, 750, 949, 988
AlwI GGATC 1 cut(s) 324
AoxI GGCC 1 cut(s) 462
ApeKI GCWGC 4 cut(s) 95, 284, 647, 1097
ApoI RAATTY 1 cut(s) 82
AspLEI GCGC 1 cut(s) 506
AspS9I GGNCC 2 cut(s) 539, 947
AsuC2I CCSGG 1 cut(s) 968
AsuHPI GGTGA 1 cut(s) 566
AsuNHI GCTAGC 1 cut(s) 368
AvaII GGWCC 2 cut(s) 539, 947
BalI TGGCCA 1 cut(s) 464
BbvI GCAGC 4 cut(s) 107, 296, 634, 1109
BccI CCATC 3 cut(s) 454, 644, 1028
BciT130I CCWGG 6 cut(s) 226, 693, 726, 752, 951, 990
BcnI CCSGG 1 cut(s) 968
BfaI CTAG 3 cut(s) 188, 369, 896
BfrI CTTAAG 1 cut(s) 515
BfuAI ACCTGC 1 cut(s) 916
BisI GCNGC 4 cut(s) 96, 285, 648, 1098
BlpI GCTNAGC 2 cut(s) 32, 288
BlsI GCNGC 4 cut(s) 97, 286, 649, 1099
Bme1390I CCNGG 7 cut(s) 226, 693, 726, 752, 951, 968, 990
Bme18I GGWCC 2 cut(s) 539, 947
BmgT120I GGNCC 2 cut(s) 539, 947
BmiI GGNNCC 2 cut(s) 231, 618
BmrFI CCNGG 7 cut(s) 226, 693, 726, 752, 951, 968, 990
BmrI ACTGGG 1 cut(s) 1039
BmsI GCATC 1 cut(s) 107
BmtI GCTAGC 1 cut(s) 372
BmuI ACTGGG 1 cut(s) 1039
BoxI GACNNNNGTC 1 cut(s) 579
BpmI CTGGAG 2 cut(s) 708, 1011
Bpu1102I GCTNAGC 2 cut(s) 32, 288
BpuEI CTTGAG 1 cut(s) 598
BpuMI CCSGG 1 cut(s) 968
BsaAI YACGTR 1 cut(s) 173
BsaJI CCNNGG 5 cut(s) 224, 225, 691, 751, 882
BsaWI WCCGGW 1 cut(s) 158
BsaXI ACNNNNNCTCC 2 cut(s) 134, 164
Bsc4I CCNNNNNNNGG 1 cut(s) 956
Bse1I ACTGG 1 cut(s) 1034
Bse3DI GCAATG 2 cut(s) 97, 660
BseBI CCWGG 6 cut(s) 226, 693, 726, 752, 951, 990
BseDI CCNNGG 5 cut(s) 224, 225, 691, 751, 882
BseGI GGATG 4 cut(s) 261, 465, 687, 1039
BseLI CCNNNNNNNGG 1 cut(s) 956
BseMI GCAATG 2 cut(s) 97, 660
BseMII CTCAG 1 cut(s) 279
BseNI ACTGG 1 cut(s) 1034
BseRI GAGGAG 2 cut(s) 55, 821
BseXI GCAGC 4 cut(s) 107, 296, 634, 1109
Bsh1236I CGCG 1 cut(s) 504
BshFI GGCC 1 cut(s) 464
BsiSI CCGG 2 cut(s) 159, 968
BslFI GGGAC 1 cut(s) 155
BslI CCNNNNNNNGG 1 cut(s) 956
BsmFI GGGAC 1 cut(s) 155
BsmI GAATGC 1 cut(s) 863
BsnI GGCC 1 cut(s) 464
Bsp143I GATC 2 cut(s) 316, 784
Bsp1720I GCTNAGC 2 cut(s) 32, 288
BspACI CCGC 1 cut(s) 502
BspANI GGCC 1 cut(s) 464
BspCNI CTCAG 1 cut(s) 280
BspFNI CGCG 1 cut(s) 504
BspLI GGNNCC 2 cut(s) 231, 618
BspMI ACCTGC 1 cut(s) 916
BspOI GCTAGC 1 cut(s) 372
BspPI GGATC 1 cut(s) 324
BspTI CTTAAG 1 cut(s) 515
BsrDI GCAATG 2 cut(s) 97, 660
BsrI ACTGG 1 cut(s) 1034
BssECI CCNNGG 5 cut(s) 224, 225, 691, 751, 882
BssMI GATC 2 cut(s) 316, 784
BssT1I CCWWGG 1 cut(s) 882
Bst2UI CCWGG 6 cut(s) 226, 693, 726, 752, 951, 990
Bst4CI ACNGT 1 cut(s) 860
Bst6I CTCTTC 1 cut(s) 416
BstAFI CTTAAG 1 cut(s) 515
BstBAI YACGTR 1 cut(s) 173
BstC8I GCNNGC 2 cut(s) 16, 370
BstDEI CTNAG 4 cut(s) 32, 198, 288, 890
BstF5I GGATG 4 cut(s) 261, 465, 687, 1039
BstFNI CGCG 1 cut(s) 504
BstHHI GCGC 1 cut(s) 506
BstKTI GATC 2 cut(s) 319, 787
BstMBI GATC 2 cut(s) 316, 784
BstMWI GCNNNNNNNGC 8 cut(s) 11, 191, 284, 445, 671, 753, 930, 1013
BstNI CCWGG 6 cut(s) 226, 693, 726, 752, 951, 990
BstNSI RCATGY 2 cut(s) 979, 1058
BstPAI GACNNNNGTC 1 cut(s) 579
BstSCI CCNGG 7 cut(s) 224, 691, 724, 750, 949, 966, 988
BstUI CGCG 1 cut(s) 504
BstV1I GCAGC 4 cut(s) 107, 296, 634, 1109
BsuRI GGCC 1 cut(s) 464
BtgZI GCGATG 1 cut(s) 1078
BtsCI GGATG 4 cut(s) 261, 465, 687, 1039
BtsI GCAGTG 1 cut(s) 1120
BtsIMutI CAGTG 2 cut(s) 865, 1120
BveI ACCTGC 1 cut(s) 916
Cac8I GCNNGC 2 cut(s) 16, 370
CfoI GCGC 1 cut(s) 506
Cfr13I GGNCC 2 cut(s) 539, 947
CseI GACGC 1 cut(s) 1057
Csp6I GTAC 1 cut(s) 995
CviAII CATG 7 cut(s) 28, 551, 868, 921, 956, 976, 1055
CviQI GTAC 1 cut(s) 995
DdeI CTNAG 4 cut(s) 32, 198, 288, 890
DpnI GATC 2 cut(s) 318, 786
DpnII GATC 2 cut(s) 316, 784
DraI TTTAAA 1 cut(s) 718
DraIII CACNNNGTG 1 cut(s) 554
EaeI YGGCCR 1 cut(s) 462
Eam1104I CTCTTC 1 cut(s) 416
EarI CTCTTC 1 cut(s) 416
Eco130I CCWWGG 1 cut(s) 882
Eco32I GATATC 1 cut(s) 625
Eco47I GGWCC 2 cut(s) 539, 947
Eco57I CTGAAG 1 cut(s) 576
EcoRII CCWGG 6 cut(s) 224, 691, 724, 750, 949, 988
EcoRV GATATC 1 cut(s) 625
EcoT14I CCWWGG 1 cut(s) 882
EcoT22I ATGCAT 1 cut(s) 1060
ErhI CCWWGG 1 cut(s) 882
FaeI CATG 7 cut(s) 31, 554, 871, 924, 959, 979, 1058
FalI AAGNNNNNCTT 2 cut(s) 375, 407
FaqI GGGAC 1 cut(s) 155
FatI CATG 7 cut(s) 27, 550, 867, 920, 955, 975, 1054
FauNDI CATATG 1 cut(s) 1044
Fnu4HI GCNGC 4 cut(s) 96, 285, 648, 1098
FokI GGATG 4 cut(s) 268, 472, 674, 1046
Fsp4HI GCNGC 4 cut(s) 96, 285, 648, 1098
FspBI CTAG 3 cut(s) 188, 369, 896
GlaI GCGC 1 cut(s) 505
GluI GCNGC 4 cut(s) 96, 285, 648, 1098
GsuI CTGGAG 2 cut(s) 708, 1011
HaeIII GGCC 1 cut(s) 464
HapII CCGG 2 cut(s) 159, 968
HgaI GACGC 1 cut(s) 1057
HhaI GCGC 1 cut(s) 506
Hin1II CATG 7 cut(s) 31, 554, 871, 924, 959, 979, 1058
Hin6I GCGC 1 cut(s) 504
HinP1I GCGC 1 cut(s) 504
HincII GTYRAC 1 cut(s) 61
HindII GTYRAC 1 cut(s) 61
HindIII AAGCTT 4 cut(s) 108, 512, 999, 1103
HinfI GANTC 3 cut(s) 143, 580, 1023
HpaI GTTAAC 1 cut(s) 61
HpaII CCGG 2 cut(s) 159, 968
HphI GGTGA 1 cut(s) 566
Hpy166II GTNNAC 1 cut(s) 61
Hpy188I TCNGA 6 cut(s) 81, 154, 250, 539, 595, 795
Hpy188III TCNNGA 5 cut(s) 188, 320, 497, 620, 1088
Hpy8I GTNNAC 1 cut(s) 61
Hpy99I CGWCG 1 cut(s) 254
HpyAV CCTTC 2 cut(s) 49, 883
HpyCH4III ACNGT 1 cut(s) 860
HpyCH4IV ACGT 1 cut(s) 172
HpyCH4V TGCA 7 cut(s) 95, 770, 848, 863, 1058, 1113, 1118
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 191, 284, 445, 671, 753, 930, 1013
HpyF3I CTNAG 4 cut(s) 32, 198, 288, 890
HpySE526I ACGT 1 cut(s) 172
Hsp92II CATG 7 cut(s) 31, 554, 871, 924, 959, 979, 1058
HspAI GCGC 1 cut(s) 504
KspAI GTTAAC 1 cut(s) 61
Kzo9I GATC 2 cut(s) 316, 784
LmnI GCTCC 3 cut(s) 41, 207, 727
Lsp1109I GCAGC 4 cut(s) 107, 296, 634, 1109
LweI GCATC 1 cut(s) 107
MaeI CTAG 3 cut(s) 188, 369, 896
MaeII ACGT 1 cut(s) 172
MaeIII GTNAC 3 cut(s) 113, 522, 832
MalI GATC 2 cut(s) 318, 786
MboI GATC 2 cut(s) 316, 784
MboII GAAGA 7 cut(s) 38, 65, 68, 212, 403, 582, 868
MfeI CAATTG 1 cut(s) 801
MlsI TGGCCA 1 cut(s) 464
MluCI AATT 5 cut(s) 82, 134, 610, 801, 1083
MluNI TGGCCA 1 cut(s) 464
MlyI GAGTC 2 cut(s) 137, 589
MmeI TCCRAC 2 cut(s) 177, 273
MnlI CCTC 4 cut(s) 76, 184, 513, 799
Mox20I TGGCCA 1 cut(s) 464
Mph1103I ATGCAT 1 cut(s) 1060
MscI TGGCCA 1 cut(s) 464
MslI CAYNNNNRTG 2 cut(s) 828, 942
Msp20I TGGCCA 1 cut(s) 464
MspA1I CMGCKG 1 cut(s) 287
MspCI CTTAAG 1 cut(s) 515
MspI CCGG 2 cut(s) 159, 968
MspR9I CCNGG 7 cut(s) 226, 693, 726, 752, 951, 968, 990
MunI CAATTG 1 cut(s) 801
Mva1269I GAATGC 1 cut(s) 863
MvaI CCWGG 6 cut(s) 226, 693, 726, 752, 951, 990
MvnI CGCG 1 cut(s) 504
MwoI GCNNNNNNNGC 8 cut(s) 11, 191, 284, 445, 671, 753, 930, 1013
NciI CCSGG 1 cut(s) 968
NdeI CATATG 1 cut(s) 1044
NdeII GATC 2 cut(s) 316, 784
NheI GCTAGC 1 cut(s) 368
NlaIII CATG 7 cut(s) 31, 554, 871, 924, 959, 979, 1058
NlaIV GGNNCC 2 cut(s) 231, 618
NmuCI GTSAC 2 cut(s) 522, 832
NsiI ATGCAT 1 cut(s) 1060
NspI RCATGY 2 cut(s) 979, 1058
PasI CCCWGGG 1 cut(s) 225
PciI ACATGT 1 cut(s) 975
PctI GAATGC 1 cut(s) 863
PfeI GAWTC 1 cut(s) 1023
PflMI CCANNNNNTGG 1 cut(s) 956
PfoI TCCNGGA 1 cut(s) 949
PkrI GCNGC 4 cut(s) 97, 286, 649, 1099
PleI GAGTC 2 cut(s) 137, 588
PpsI GAGTC 2 cut(s) 137, 588
Ppu21I YACGTR 1 cut(s) 173
PscI ACATGT 1 cut(s) 975
PshAI GACNNNNGTC 1 cut(s) 579
PsiI TTATAA 1 cut(s) 732
Psp6I CCWGG 6 cut(s) 224, 691, 724, 750, 949, 988
PspGI CCWGG 6 cut(s) 224, 691, 724, 750, 949, 988
PspN4I GGNNCC 2 cut(s) 231, 618
PspPI GGNCC 2 cut(s) 539, 947
PvuII CAGCTG 1 cut(s) 287
RsaI GTAC 1 cut(s) 996
RsaNI GTAC 1 cut(s) 995
RseI CAYNNNNRTG 2 cut(s) 828, 942
SatI GCNGC 4 cut(s) 96, 285, 648, 1098
Sau3AI GATC 2 cut(s) 316, 784
Sau96I GGNCC 2 cut(s) 539, 947
SchI GAGTC 2 cut(s) 137, 589
ScrFI CCNGG 7 cut(s) 226, 693, 726, 752, 951, 968, 990
SfaNI GCATC 1 cut(s) 107
SinI GGWCC 2 cut(s) 539, 947
SmiMI CAYNNNNRTG 2 cut(s) 828, 942
SmlI CTYRAG 2 cut(s) 515, 577
SmoI CTYRAG 2 cut(s) 515, 577
Sse9I AATT 5 cut(s) 82, 134, 610, 801, 1083
SsiI CCGC 1 cut(s) 502
SspMI CTAG 3 cut(s) 188, 369, 896
StyD4I CCNGG 7 cut(s) 224, 691, 724, 750, 949, 966, 988
StyI CCWWGG 1 cut(s) 882
TaaI ACNGT 1 cut(s) 860
TaiI ACGT 1 cut(s) 175
TaqI TCGA 1 cut(s) 241
TasI AATT 5 cut(s) 82, 134, 610, 801, 1083
TatI WGTACW 1 cut(s) 994
TfiI GAWTC 1 cut(s) 1023
TscAI CASTG 2 cut(s) 865, 1120
TseFI GTSAC 2 cut(s) 522, 832
TseI GCWGC 4 cut(s) 95, 284, 647, 1097
Tsp45I GTSAC 2 cut(s) 522, 832
TspDTI ATGAA 4 cut(s) 16, 492, 523, 856
TspGWI ACGGA 1 cut(s) 357
TspRI CASTG 2 cut(s) 865, 1120
Van91I CCANNNNNTGG 1 cut(s) 956
Vha464I CTTAAG 1 cut(s) 515
VpaK11BI GGWCC 2 cut(s) 539, 947
XapI RAATTY 1 cut(s) 82
XbaI TCTAGA 1 cut(s) 187
XceI RCATGY 2 cut(s) 979, 1058
XspI CTAG 3 cut(s) 188, 369, 896
Zsp2I ATGCAT 1 cut(s) 1060
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.